Saa Taa Ni
Saa Taa Ni
3
Edited by Dr Ipek Kurtböke, University of the Sunshine Coast, Australia
WORLD FEDERATION FOR CULTURE COLLECTIONS http://wdcm.nig.ac.jp/wfcc/
3. WFCC will make an effort to propose a require it. The OECD has created a web based
standard minimal MTA safeguarding the information resource (http://www.biosecurity.org).
interest of all stakeholders
The WFCC is working with various project consortia and
The European Culture Collection Organisation (ECCO) organisations to develop a practical way forward for
has prepared core text http://eccosite.org for collections in biosecurity risk assessment. The EMbaRC
implementation by collection members; this text should project http://www.embarc.eu funded under the EU 7th
be considered by all WFCC member collections. A paper Framework Programme (Grant agreement number: FP7-
was submitted to COP10 to present the WFCC position 228310) is designed to help deliver a code of conduct
(Smith & Desmeth, 2007) and Philippe Desmeth has and mechanisms for risk assessment. The GBRCN is
represented WFCC at subsequent ABS meetings. working with EMbaRC to help microbial resource
collections implement biosecurity best practice.
Smith, D. & Desmeth, P. (2007). Access Together they hope to develop common methodologies
and benefit sharing, a main preoccupation for risk assessment and seek quantitative and qualitative
of the World Federation of Culture tools and assessments that assist in completing
Collections. In: UNEP/CBD/WG- appropriate and comparable risk assessment. A data
ABS/6/INF/3 13 December 2007 base that BRCs may use as a reference will be
Compilation of submissions provided by developed leading to an increasingly harmonized
parties, governments, indigenous and framework of risk assessment and risk management.
local communities and stakeholders on The GBRCN Demonstration Project web site provides on
concrete options on substantive items on biosafety, biosecurity and transport (of biological
the agenda of the fifth and sixth meetings materials) and the implementation of best practices
of the ad hoc open ended working group amongst other items. Links to the EBRCN Information
on access and benefit sharing. Canada: resource, Code of practice for International Depositary
UNEP/CBD. p 68-70. Authorities, WHO Biosafety Manual and other key
operational documents and information are given at:
http://www.gbrcn.org/project/further-reading.html.
4. WFCC will make an effort to create a
workable solution for curators to aid in
Additionally, partners were provided with the publication
risk assessment imposed by biosecurity
BG Chemie Merkblätter to help in the process of risk
regulations (dual-use).
assessment. Both EMbaRC and GBRCN will provide
information for the benefit of all WFCC collections.
Amongst all different legal areas within bio-legislation, Further discussions will be held at ICCC12.
biosecurity is probably the most complex and difficult
aspect. As national controls are put in place through 5. WFCC will collaborate with initiatives such
national legislation, for example the Patriot Act in the as Straininfo.net and Mycobank to
USA and Security Act in the UK, the lists of organisms of improve the quality and validity of strain
concern differ. In a spectrum of risk, spanning natural data placed in the public domain
events, from emerging disease through man’s
intervention (e.g. laboratory accidents to deliberate acts, An experiment is being carried out between
to bioterrorism) the greatest risk comes from emerging straininfo.net and the EMbaRC and GBRCN projects to
disease. Control of access to microorganisms and their give access to WFCC, EMbaRC and GBRCN partner data
safe handling has been in place for many years and is via specific portals. This is paralleled by an experiment
subject to national laws. The OECD Biological Resource broadening the use of the Brazilian Network software
Centre (BRC) Guidelines (OECD, 2007) includes SiCOL. The results of these experiments will be reported
guidance to deliver a practical approach that enables at ICCC12. In addition, the Common Access to Biological
legitimate research and development but reduces the Resources and Information (CABRI) guidelines for
opportunity for misuse. The OECD BRC Task Force catalogue production will be converted into a community
agreed that guidance is necessary but that it should not standard for culture collection data management and
be bureaucratic and applied to situations that don’t delivery.
4
Edited by Dr Ipek Kurtböke, University of the Sunshine Coast, Australia
WORLD FEDERATION FOR CULTURE COLLECTIONS http://wdcm.nig.ac.jp/wfcc/
6. WFCC will work with ICSB to deposit long- because mBRCs very often refuse acceptance of
term preserved type strains of fungi in taxonomically poorly characterized strain assemblages,
minimal two different collections in mostly isolated in the course of field work. Authors,
different countries. requested to deposit into mBRCs selected strains
included in the scientific literature, are lacking assistance
7. As type strains are the property of the regarding the number and taxon to deposit and they will
international scientific community the face a negative response from resource centers unless
WFCC will work to ensure they remain the relevant strain ranges within the specific interest of
available to qualified workers without a mBRC. There is also resistance to deposit strains into
restrictions or impediment. mBRCs, an attitude still in the process of being
investigated. On the other hand, the user is increasingly
The WFCC Board is working on a paper with Brian Tindal dissatisfied with the range of resources offered by
of the ICSP entitled Access to Type strains and mBRCs. While there is excellent coverage for type and
equivalent reference strains from service culture reference strains, specific
collections which is yet to be submitted and covers
aspects of resolutions 6 and 7. Footnote:
At this point, the mode and technicalities of the
There is still a lot of work to be done on these issues transformation of public collections sensu pre-OECD-best-
and it will continue under the new WFCC Board and the practice-era into Biological Resource Centres (BRC) sensu
above mentioned project consortia as their projects and post-OECD-best-practice-era
(http://www.gbrcn.org/project/further-reading.html) are
initiatives develop. under discussion. For the ease of communication the term
microbial Biological Resource Centres (mBRC), denoting a
quality assured collection, will be used.
A strategy for improving the use of
microbial Resource Centres (mBRCs) strains of current scientific interest, plasmids, phages
and mutants included in research are hardly available.
1 2 3
Erko Stackebrandt , Sylvie Lortal , Chantal Bizet , David Alongside these issues, mBRCs are already working at
4 5 the limits of financial capacity, available space,
Smith , Philippe Desmeth ,
6 7
Esperanza Garay Aubán , Nelson Lima and Joost equipment, expertise and, consequently, expansion of
8
Stalpers holdings. Microbial RCs mainly concentrate either in
providing a broad range of biodiversity, often restricted
1 to type and reference strains, or they focus on a specific
, DSMZ, Braunschweig, Germany, range of taxa with interest for medicine, agriculture,
2
, CIRM INRA, Rennes, France pharmaceutical or food sectors. There is even a fourth
3
, CRBIP, Institut Pasteur, Paris, France,
4 element impacting on this situation, namely the national
, CABI Europe (UK), Egham, Surrey, UK,
5 granting bodies. Only today, almost 20 years after
, BCCM Coordination Unit, Brussels, Belgium
6 signing the Convention on Biological Diversity
, UVEG-CECT, Valencia, Spain
7
Micoteca da Universidade do Minho (MUM), IBB/Centro (http://www.cbd.int/), governments are slowly
de Engenharia Biológica, Braga, Portugal recognising the added value of a wide access to
8
, CBS, Utrecht, The Netherlands microorganisms, to those living resources for which
associated bio-informatics data are already available,
either by published sources or by data mining of
networked information.
At a time of increasing control of use of biological
materials be it by countries executing sovereign rights of
researchers generating intellectual property a growing Obviously, this vicious circle needs to be forced open in
uneasiness can be observed among the providers and order to satisfy all stakeholders involved. The question is
the users of microbial resources and the curators of at which point should the circle be cracked? Neither
public collections/BRCs (BRC and mBRC - see footnote). editors nor curators have the power to request
Within the group of provider we distinguish between mandatory strain deposition. As voluntary deposition
researcher and author, although being aware that an does not work (as clearly shown by past and present
author is also a researcher. Researchers complain experience), who then should be in the position to
5
Edited by Dr Ipek Kurtböke, University of the Sunshine Coast, Australia
WORLD FEDERATION FOR CULTURE COLLECTIONS http://wdcm.nig.ac.jp/wfcc/
advise the authors and other scientists which fraction of term storage, IP, MTA, biosafety and biosecurity,
strains to deposit? It should be very clear that we are import/ export and shipping issues and strain
talking only about a fraction of published strains; it also identification approaches.
goes without saying that without a qualified assessment
While a scientist in charge of a research collection
of the strain selected for deposition resource centres
should feel free to contact a collection anytime (either
would be overburdened in no time. The result of a
direct or via the AmBRCA), an author has to wait for the
recent questionnaire to authors (Stackebrandt,
result of the reviewing process. Already at the first
unpublished: see also below) on this matter made it very
revision stage the editor should link the corresponding
clear that authors, in concert with editors and curators
author with the AmBRCA webpage to trigger further
of public collections should be responsible for the
actions. In an optimal situation, following the adaptation
selection: authors, because they (mostly together with
phase, advice on potential strains to deposit could also
the host institution) own the intellectual property rights
come from the reviewers, directed either to the editor or
and they should have the right to exclude those
to the author by the revision report. Here, the journals
'scientifically hot' strains which are still under
could add a relevant item to the score sheet.
investigation; editors, because they should catalyze the
connections between authors and curators, at least as So far, the conceptional development of a multi-facet
long as a network of public collection accepting mechanism is just a construct of ideas that should work
reference strains included in the literature has not been in principle. What is missing, however, is firstly the
established; and curators, because they will evaluate the willingness of all stakeholders involved that the
author's strains in the light of existing gaps in holdings increased deposition and public provision of microbial
of particular taxa. Interestingly, scientists would not like resources adds value to scientific progress and
to see granting bodies to have a say in the selection of stimulates the bio-economy. Secondly, each step in this
strains to deposit. network of interactions is costly in terms of time and
expense. Maintenance of the AmBRCA network, strain
The evaluation of gaps in and strength of, individual
identification, shipping, strain authentification long-term
collections requires a thorough screening of existing
maintenance and electronic catalogue entries are fixed
holdings and expertise at the international level. While
costs which differ from species to species and from
certain national (e.g., Belgium, France UK, USA, Brazil,
mBRC to mBRC.
Japan, Korea) and regional networks (e.g., Europe, Asia)
are presently in the process of inventorying and The question that immediately comes up is: who is
comparing their collections, the range of resource going to pay for this (the reader has probably asked this
centres need to be broadened in order to be able to question much earlier). The question concerning
cope with the expected increase. A centralized coverage of arising cost was asked in the above
professional secretariat, such as that presently being mentioned questionnaire and the response of the
established for the Global Biological Resource Centre authors was univocal: additional costs for strain
Network (GBRCN; http://www.gbrcn.org/) should be identification, shipping and partly for long-term
trustworthy and accepted by all partners. We hesitate to maintenance should be covered by research grants,
assign this role to the World Federation of Culture which mostly comes from national research agencies. It
Collection (WFCC) as this IUMS Federation makes sense to allocate a certain fraction of grants for
(http://www.wfcc.info/index.html) lacks a tightly this purpose, as the professional maintenance of
organized structure needed to fulfil the role of a broker biological material, isolated with tax payers money,
between various stakeholders. Whatever structure will should be of national interest. Research agencies are in
emerge at the end, a gateway giving access to a a powerful position to link the notification of the
centrally maintained database (AmBRCA: Access to granting of funds with the obligation to firstly properly
microbial resource centres and Advice) is needed in maintain strain collections for future adoption by mBRCs,
order to guarantee a smooth dialog between partners and secondly to deposit relevant (depending on the
involved. This database should comprise lists of (i) those outcome of the discussion at the AmBRCA level) strains
mBRCs agreed to work towards a better coverage of included in the scientific literature in public mBRCs.
microbial diversity; (ii) genera and species the Financial support for the necessary expansion of the
participating mBRCs agree to cover; (iii) contact persons latter institutes will not be covered by research grants
for rapid evaluation of external strain collections; and but must come from the mother organisations or
experts for advice on e. g., taxonomic questions, long funding bodies of mBRCs (the responsibilities of
6
Edited by Dr Ipek Kurtböke, University of the Sunshine Coast, Australia
WORLD FEDERATION FOR CULTURE COLLECTIONS http://wdcm.nig.ac.jp/wfcc/
governments, having signed the CBD, to care for ex-situ NEWS FROM MEMBERS
conservation of their national biological material, could
be used as an additional argument). It should be
mentioned that the number of strains deposited outside New Head of the DSMZ
the public domain, i.e., research collections as well as Jörg Overmann
the number of strains covered annually in the scientific
literature has not been evaluated but one can assume
that they constitute a major addition to the about
1.240.000 bacteria and fungi deposited in those
collection which are registered in the World Data Centre
for Microorganisms
(http://www.wfcc.info/datacenter.html). For the
continuous functioning of mBRCs - and many of these
are already now working at the limits of their capacities
- financial support for technical and personnel expansion
is absolutely essential. Acknowledgement of these
essential preconditions by the relevant granting bodies
for research and mBRCs is a conditio sine qua non for a
successful implementation of the mechanisms outlined
above.
7
Edited by Dr Ipek Kurtböke, University of the Sunshine Coast, Australia
WORLD FEDERATION FOR CULTURE COLLECTIONS http://wdcm.nig.ac.jp/wfcc/
Importance and Role of a Service or P3 labs and Bio-safety Laboratories (BSL 3 or 4) are
used. Negative pressure is maintained and air circulation
Culture Collection is through HEPA filters in BSL. The entry to these BSLs
by the workers is restricted and strict security is
maintained to prevent misuse of these disease causing
cultures. Entry cards to the bio-safety facility are
provided only to authorized persons and close circuit
cameras are fixed for electronically monitoring activity in
the BSL area and on the entry/exit points.
NIICC WDCM961 NII Microbial Culture Collection These culture collections apart from preserving cultures
and providing services related to culture collections also
Culture Collection, Microbiology and Cell
NTCCI WDCM107 undertake research projects in different areas like
Biology Laboratory
environmental biotechnology, ecology, taxonomy. The
RRJ WDCM846 RRL , Jammu INDIA collections having medically important microbes are
distributed all over the country. There is no centralized
Food and Fermentation Technology Division, culture collection of medically important microbes. I
UMFFTD WDCM562
University of Mumbai
have suggested to ICMR to have one such centralized
VISVA-BHARATI CULTURE COLLECTION OF culture collection of medically important microbes so
VBCCA WDCM931
ALGAE that strains are housed in one central place and are
easily accessible to researchers. Due to good team
VPCI WDCM497 Fungal Culture Collection efforts, MTCC located in the Institute of Microbial
Technology (IMTECH) Chandigarh has been elevated to
an IDA on 4 October, 2002, thus becoming the first IDA
Many countries have sometimes several culture in India. To increase exploration of Indian ecosystems
collections that are service collections again and improve through put in screening programmes
demonstrating the vital roles they have in conserving Indian researchers and collections should play their
9
Edited by Dr Ipek Kurtböke, University of the Sunshine Coast, Australia
WORLD FEDERATION FOR CULTURE COLLECTIONS http://wdcm.nig.ac.jp/wfcc/
part. Despite the rich biodiversity of India little is ANNOUNCEMENTS
available in the culture collections (see Table 1).
Awareness of the role and importance of strain deposits
in culture collections must be raised by the government
on a large scale. Further, publicly funded research
should result in more of the Indian microbial diversity
being available and deposited in the collections.
Collaborations must be encouraged and well funded so Grants for access to leading EU Microbial Biological
that the exploratory work is enhanced in the various Resource Centres (BRCs)
corners of the country. People with expertise in isolation
and screening of microorganisms for useful compounds
The EMbaRC Training and Outreach Programme (TOP) is
should work with researchers, to learn from their an opportunity for scientists* to stay at one of the
experiences and to ensure that the research heads in
EMbaRC centres and benefit from expert advice and
the right direction. The culture collections listed in Table advanced equipment. EMbaRC will cover the bench fees,
1 must gear up to take up the challenge to conserve and travel and subsistence costs. For more information and
study the rich and possibly unique microbial biodiversity
application form, visit the TOP webpage at:
for production of novel compounds. www.embarc.eu
The author is a Fellow AMI, FNABS, NESA
This unique opportunity for training in collection
Environmentalist and Member WFCC Task Groups. He
management, identification of bacteria and fungi by
joined MTCC/IMTECH in 1987 in the formative years. He
state-of-the-art techniques or phenotypic screening of a
dedicates the article to his father Mr. Darshan Singh, ex-
collection of strains is organised with the support of the
Secretary Law & Justice and Legal Remembrancer.
Seventh Framework Programme, Research
Infrastructures Action.
References:
1. http://wdcm.nig.ac.jp/hpcc.html *scientists who work in a institution established in a EU
Member State or FP7 Associated Country (Albania,
2. Preservation of microorganisms as deposits in Bosnia & Herzegovina, Croatia, Iceland, Israel,
Patent Applications. Swaranjit Singh Cameotra. Liechtenstein, Former Yugoslav Republic of Macedonia,
BBRC 353(4):849-50. Feb 23, 2007. Montenegro, Norway, Serbia, Switzerland and Turkey).
4. http://plantbreeding.org/article.php?story_id=
RETURN OF PHAGES
296&slashSess=e5d32c38cb154a98d6c58ac2487
İpek Kurtboke
dacef
University of the Sunshine Coast
Australia
5. Microbial Culture Collections: Their Activities and
Importance Swaranjit Singh Cameotra. In
Due to the increasing antibiotic resistance among
Microbes: Agriculture, Industry and Environment
pathogenic bacteria and emergence of pathogens
pp. 261-269 (2000).
bacteriophages have been in medicinal use in the former
Soviet Union and Eastern Block countries are making a
6. Geographical Indications. Swaranjit Singh
fascinating return to the West for applications. Eliava
Cameotra. CSIR NEWS Progress, Promise and
Institute in Tbilisi, Georgia has been one of the institutes
Prospects, 60, 5-6, 2010
around the world where the technology has been used
even in most difficult times since 1923.
10
Edited by Dr Ipek Kurtböke, University of the Sunshine Coast, Australia
WORLD FEDERATION FOR CULTURE COLLECTIONS http://wdcm.nig.ac.jp/wfcc/
and commercial enterprises are including bacteriophages Phages are important in the world's biodiversity and
in their programs. I would like to a number of highly regulatory factors in microbial ecology and
publications and announcements to the attention of the communities, not to forget their future potential in
members; medicine, biotechnology and basic research.
13
Edited by Dr Ipek Kurtböke, University of the Sunshine Coast, Australia
WORLD FEDERATION FOR CULTURE COLLECTIONS http://wdcm.nig.ac.jp/wfcc/
REFERENCE STRAIN CATALOGUE b. Filamentous Fungi and Yeasts
PERTAINING TO ORGANISMS FOR 2. Strains Listed by WDCM Number
3. List of Collections and contacts
PERFORMANCE TESTING CULTURE If this works well the ISO joint working groups will
MEDIA consider broadening this use across other standards.
One of the key functions of the WFCC member Please visit the website and provide feedback to the
collections is to provide authentic reference strains for WFCC Board.
various purposes; one of the key areas is in proficiency
testing and in the implementation of standards. The CONFERENCES AND WORKSHOPS
appropriate strains are held by many culture collections
over the world but only a few strain numbers are
referenced in the test specifications. It is essential that ICCC-12 CONFERENCE
the strains used are appropriate and are stable lines of
those originally selected. In response to a request by the Florianópolis, Santa Catarina, Brazil September
ISO Joint Working Group 5 and the ICFMH-WPCM the 26-October 1, 2010
WFCC have created an online catalogue to facilitate
access to strains used for performance testing of culture http://www.iccc12.info/index
media.
1
4th LYOPHILISATION CONFERENCE
ISO/CD 11133:2009, Annex E. Test microorganisms for commonly
used culture media (giving information on the culture medium, culture 7-9 September 2010
conditions, test microorganisms, culture collection number of test The Window, 13 Windsor Street,
organisms and the expected reactions)
Islington, London, UK
1
Corry, J E L, Curtis, G D W and Baird, R M (Eds) Handbook of Culture Contact Information: Jacqueline Alvarado
Media for Food and Water Microbiology. Royal Society of Chemistry, In
Tel: +44 (0)20 7549 9946
preparation.
[email protected]
14
Edited by Dr Ipek Kurtböke, University of the Sunshine Coast, Australia
WORLD FEDERATION FOR CULTURE COLLECTIONS http://wdcm.nig.ac.jp/wfcc/
IBBS DISINFECTION AND DECONTAMINATION Session 1 was devoted to presenting actual
CONFERENCE developments in Turkey with a view to microbial
collections. The lectures covered purely scientific as well
28-29 September 2010 as application and production aspects of e.g. algae, and
University of Central Lancashire, Preston, UK theoretical deliberations on how to structure regional
www.ibbsonline.org collections and the relationship of general and national
culture collections.
3rd INTERNATIONAL CONFERENCE ON DRUG A Round Table was addressing the challenges
DISCOVERY AND THERAPY microbial collections have to face in the areas of future
scientific demands and increasing regulatory pressure.
7-10 February 2011 Representatives of microbial associations / federations
Dubai, UAE discussed together with curators how to react to such
www.icddt3-02.com challenges. Also discussed was how collections could
make themselves, their services and know-how more
visible, and how to further the dialogue with the various
user communities.
ECCO NEWS
An Extra Session made it possible to present plans for
a major global project in the sequencing-of-full-
The 29th Meeting of the European genomes-area (MEP) in which ECCO collections were
Culture Collections' Organisation invited to participate. In cognition of the diversity of
holdings and long tradition of efforts towards quality of
material and data in European microbial collections, the
consortium of MEP expressed their strong interest in
collaboration with ECCO collections.
This year the annual meeting of ECCO took place in the Session 3 was devoted to modern developments in
wonderful city of Istanbul in the Marmara Hotel at methodologies for strain determination, diagnosis,
Taksim Square. The local hosts took every chance to identification and typing; also an example of DNA-
make this meeting a successful one - scientifically as barcoding of strains was presented.
well as socially.
Details of the Programme
The meeting was opened by the Vice President of
Istanbul University, Çiğdem Kayacan, followed by Session 1: Culture Collections in Turkey; Chairs: Bülent
Professor Gürler, President of KÜKENS and Head of the Gürler, Seray Özensoy Toz
local organising committee, and Dagmar Fritze, Genotypic Differences of Leishmania Strains Isolated
President of ECCO. From Turkey; Seray Özensoy Toz
A Strategy for Structuring Regional Culture Collection of
The organisers had compiled a very interesting Clinical Origin; İ. Çağatay Acuner
programme spanning a wide range of scientific, Importance of the National Culture Collections; Demet
organisational, regulatory and political topics. Yumuşak
Ege University Microalgal Culture Collection (Ege-MACC)
and Research Fields; Meltem Conk Dalay
15
Edited by Dr Ipek Kurtböke, University of the Sunshine Coast, Australia
WORLD FEDERATION FOR CULTURE COLLECTIONS http://wdcm.nig.ac.jp/wfcc/
Round Table: Challenges for the Microbial Resource
Centres in the XXI Century; Chairs: Esperanza Garay,
Gerard Verkleij; Guests: Zeynep Gulay, Milton Da Costa.