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High Performance Liquid Chromatography, Altus A-10 Perkin Elmer

The document describes the components and process of high performance liquid chromatography (HPLC) using an Altus A-10 Perkin Elmer unit. It lists the main components as solvent reservoirs, a solvent degasser, gradient valve, mixing vessel, high-pressure pump, switching valve, sample injection loop, guard column, analytical column, detector, and data acquisition system. It then outlines the three main steps of sample preparation, running the analysis using Empower software, and post-analysis review of results.

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Zuhri Umar Ma'ruf
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869 views4 pages

High Performance Liquid Chromatography, Altus A-10 Perkin Elmer

The document describes the components and process of high performance liquid chromatography (HPLC) using an Altus A-10 Perkin Elmer unit. It lists the main components as solvent reservoirs, a solvent degasser, gradient valve, mixing vessel, high-pressure pump, switching valve, sample injection loop, guard column, analytical column, detector, and data acquisition system. It then outlines the three main steps of sample preparation, running the analysis using Empower software, and post-analysis review of results.

Uploaded by

Zuhri Umar Ma'ruf
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as ODT, PDF, TXT or read online on Scribd
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High Performance Liquid Chromatography, Altus A-10 Perkin Elmer

Schematic representation of an HPLC unit.

(1) Solvent reservoirs,


(2) Solvent degasser,
(3) Gradient valve,
(4) Mixing vessel for delivery of the mobile
(6) Switching valve in "inject position",
phase, (5) High-pressure pump,
(6') Switching valve in "load position",
(7) Sample injection loop,
(8) Pre-column (guard column),
(9) Analytical column,
(10) Detector (i.e. IR, UV),
(11) Data acquisition,
(12) Waste or fraction collector.
Analysis using empower software from waters over view :
Analysis process :
1. Pre analysis (Sample & Solvent Preparations)
 Making solvent
 Filtering sample & solvent
 Degassing sample & solvent
2. Run analysis (Methode setup & run sample)
 Power supply on
 Computer on
 Main instrument on (sampler and solvent module)
 Detector on (wait until lamp flash off)
 Open empower software on computer desktop
 Log in system
 Method setup
 Run sample
3. Post analysis (reprocess, Review & report)
 process data
 review
 adjust integration & identification
 save result
 preview result
 print result
 log out system
 close empower software
 main instrument off
 detector off
 computer off

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