DA99
Lifting Mechanism
DA99 Chitosan lifting ampoule skin improvement process Source: see pages 14 to 27 of this document
- When skin immunity is broken (PH, oil content, keratin, water content)
- Lack of skin benefit bacteria and proliferation of harmful bacteria
Before use
Characteristics and function of chitosan
- DA99 (Deacetylation99) High purity chitosan
- Chitosan is a substance with natural positive charge, which binds and inhibits negative charged hazardous substances.
- Creation of an apoptotic environment through inhibition of the growth of negative charged harmful bacteria (Sources 1,2,5,6,13)
- Chitosan helps to restore the weak acidity of the skin by feeding to the skin beneficial bacteria.
- Chitosan improves skin harmful environment by adsorbing and removing fine dust heavy metals in the skin. (Sources 6, 11, 12, 13)
- Improvement of skin moisture (Sources 1, 3, 7 and 9)
- Skin barrier formation (Sources 1,2,3,4)
- Chitosan is a harmless and non-toxic substances to the human body. (Sources 1,4 and 5)
- Skin regeneration function such as wrinkle improvement (Sources 2,7)
- Wrinkle ironing effect by lifting support membrane which lasts for sleeping time
In use
Major functional materials and functions
- Arbutin: Brightening
- Adenosine: Wrinkle improvement
- Ginseng fruit extract: Skin regeneration
- Rose Yobu Jericho extract: Moisturizing
- Spirulina extract: Brightening, Skin elasticity
- Hyaluronic acid: Moisturizing
- Vitamin C derivatives: Brightening, Resilience recovery
- Protein Peptide: Elastic recovery
- Skin absorption of the active ingredient maintained by the chitosan protective film during sleeping time
After use
- Skin that has undergone a recovery process should be cleaned with non-irritating water that does not use soap.
- Skin protection by moisturizing and nourishing cream (Maintain normal management method)
Maintenance Repeat skin elasticity recovery process through periodic use more than using once a week
DA99 chitosan lifting ampoule skin improvement
Damaged skin Improved skin
Skin immune barrier destruction (keratin) Initiation of skin immune barrier recovery
Reduction of skin beneficial bacteria (28-day skin regeneration cycle)
(weakly acidic destruction) Increased activity of skin-beneficial bacteria
Increase of skin harmful bacteria (trouble) (weakly acidic PH recovery started)
Heavy metal fine dust causes skin irritation. Suppression of skin harmful bacteria activity and creation
Lack of water, lack of elasticity, etc. of an apoptotic environment
Removal of heavy metal fine dust adsorption
Skin moisture improvement, elasticity improvement, etc.
DA99 chitosan lifting ampoule skin improvement 12 week program
Resilience recovery level
- Depending on the age and skin condition, the level of recovery is different.
- In the case of deep wrinkles more than age 50, it is necessary to concentrate
more than 2 ~ 3 times a week in the first month.
Damaged
Skin Level
12 week program based on using once a week Maintenance care phase
DA99 lifting ampoule is not a medicine.
It is a cosmetic product that needs constant skin elasticity maintenance care
even if your skin elasticity is restored through temporary or long-term program.
DA99 chitosan lifting ampoule skin improvement program application process _
recovery and maintenance
Maintenance: Flexibility management and continuous Recovery: Skin improvement process
maintenance care more than once a week
Stepwise skin condition
Damaged skin before application Skin immune barrier destruction (keratin)
Reduction of skin beneficial bacteria
(destruction of weakly acidic state)
Increase skin harmful bacteria (trouble)
Heavy metal fine dust skin irritation
Not enough moisture
Lack of elasticity
Stepwise skin condition
Product Application_ After Cleansing
Formation of chitosan skin
barrier with active ingredient
Apply well-melted product evenly to skin.
Stepwise skin condition
Product applied _ During sleep
Suppresses the activity of
harmful bacteria in the skin
and removes contaminants.
키토산 관련 논문
After applying the
product, take a sleep
while lifted.
Stepwise skin condition
Product applied _ During sleep
Activation of
skin benefit bacteria
Chitosan becomes a food of skin-beneficial
bacteria, and the beneficial germ is
activated.
After applying the
product, take a sleep
while lifted.
Stepwise skin condition
Product applied _ During sleep
The process of restoring skin
elasticity is going on.
Effect of active substances such as Arbutin, Adenosine,
Ginseng fruit extract, Hyaluronic acid, Vitamin C
derivatives, Protein, etc.
After applying the
product, take a sleep
while lifted.
Stepwise skin condition
Product Application_Water cleansing
Water cleansing
Wash with water without using alkaline
soap to restore skin barrier.
Wash the remaining lifting
support membrane with water
only after getting up in the
morning.
Stepwise skin condition
After applying the product
Skin care and
management
Please skin care as usual with
sunscreens, moisturizers, etc.
Use your usual skin care products.
Stepwise skin condition
Periodic management
more than using once a week The skin will repeat its regeneration cycle
based on 28 days.
Constant and ongoing care is important to
create a resilient healthy skin.
The DA99 lifting ampoule recommends a 3-
month wrinkle restoration program (12 weeks)
and focus on maintenance care even after
recovery.
Contents Source1 [Journal of Scientific & Industrial Research / 2004]
Harmless for human body / nontoxic / Anti-bacterial effect / Velum (skin protection membrane) forming
Source2 [Reactive & Functional Polymers 46 (2000) 1–27 / 2000]
• Antimycotic and corium protection ingredient / Ingredient helping skin regeneration through anti-bacterial effect
Source3 [Korea Chitin Chitosan Research Paper, 2(4), 5-13, 1997 / 1997]
Same moisturizing effect with propylene, glycol, glycerine
Source4 [Korea Chitin Chitosan Research Paper, 2(4), 5-13, 1997 / 1997]
Ingredient with skin dryness prevention function
키토산 관련 논문
Source5 [Food Science, 2010, Vol. 31, No. 13, Tianshui normal University / 2010]
Anti-bacterial characteristics and restraining growth of bacteria / Destroys bacteria cell barrier with high penetrability
Source6 [J. of Korean Ind. & Eng. Chem., Vol. 9, No. 4, Augustl 1998, 504-508 / 1998]
Heavy metal adsorption function
Source7 [Korea Chitin Chitosan Research Paper, 2(4), 5-13, 1997]
Skin beauty care effect of chitosan-Skin moisturizing
Source8 [Engineering Master’s Thesis, Graduate School of Industry, Seoul National University of Technology / 2010]
Anti-bacterial effect of chitosan
Source9 [Master’s degree application thesis, Graduate School, Ewha Woman’s University / 1993]
Moisture keeping ability of chitosan
Source10 [Master’s degree application thesis, Graduate School, Ewha Woman’s University / 1993]
Adsorption of chitosan
Source11 [Master’s degree application thesis, Graduate School, Sungsin Woman’s University / 2001]
Heavy metal adsorption and removal of chitosan
Source12 [Master’s degree application thesis, Graduate School, Sungsin Woman’s University / 2001]
Heavy metal adsorption and removal of chitosan
Source13 [Master’s degree application thesis, Graduate School, Bookyung University / 2002]
Anion adsorption ability of chitosan / Moisture and fat adsorption ability of chitosan
Result of study on universal
characteristics of chitosan
• Harmless for human body / nontoxic
• Anti-bacterial effect
• Velum (skin protection membrane) forming
• Hemostatic function
• Cartilage regeneration
Result of study on raw material for
cosmetics
• Moisturizing function
Source : Journal of Scientific & Industrial
Research / 2004
Excellent raw material for cosmetics
• Antimycotic and corium protection ingredient
• The one and only cation resin ingredient that
becomes to have viscosity encountering acid
Nature with good regeneration function
• Ingredient helping skin regeneration through anti-
bacterial effect
Source : Reactive & Functional Polymers 46
(2000) 1–27 / 2000
Outstanding moisturizing effect
• Same moisturizing effect with propylene, glycol,
glycerine, etc. that are used a lot as general
moisturizer
Chitosan has good moisturizing effect, and
especially different from existing moisturizers in
that, it is cation high molecule, so it is strongly
attached to hair or skin surface to form membrane
effectively maintaining moisture, thus having
advantage of continuing the moisturizing effect for
a long period of time.
Source: Korea Chitin Chitosan Research Paper
, 2(4), 5-13, 1997 / 1997
Velum forming
• Ingredient with skin dryness prevention function
One of the general characteristics of high
molecule is the forming of membrane, and
chitosan can also form good velum in adequate
condition. This has high affinity to hair and skin,
and you can get velum that is difficult to take off.
Such characteristic can be used as hair resin or
skin protection membrane.
Source: Korea Chitin Chitosan Research Paper
, 2(4), 5-13, 1997 / 1997
Anti-bacterial characteristics and
restraining growth of bacteria
Destroys bacteria cell barrier with
high penetrability
Source : Food Science, 2010, Vol. 31, No. 13,
Tianshui normal University / 2010
Heavy metal adsorption function of
chitosan
Heavy metal adsorption ratio of chitosan was
highest for Fe2 with 99.72%, and Cu2 99.64%,
Pb2 99.26%, Zn2 97.24%, Cd2 94.3%, Mn2
84.69%, Ni2 83.68%, and Cr6 30.32%.
Source: J. of Korean Ind. & Eng. Chem., Vol. 9,
No. 4, Augustl 1998, 504-508 / 1998
Skin beauty care effect of chitosan
The categories anticipated for effectiveness with
CM-Chitin as raw material for cosmetics are skin
moisturizing, face wrinkle care, rough skin care,
and hair protection, etc.
1.Skin moisturizing
(2) When CM-Chitin is compared to propylene
glycol and hyaluronic acid, which are generally
used as moisturizer currently, using moisture
measurement device on each layer, first, when
each sample is painted out and the moisture
amount on the skin surface is measured with
electricity conductivity as time goes, CM-Chitin
was found to have equivalent or better
moisturizing ability than the other two samples.
Source: Korea Chitin Chitosan Research Paper
, 2(4), 5-13, 1997 / 1997
Anti-bacterial effect of chitosan
Chitosan derivative CsAa showed anti-bacterial effect
against Streptococcus Pyogenes of 95.4%,
Staphylococcus aureus of 99.4%, and Escherichia coli
of 95.8% at supply concentration of 300ppm, and at
supply concentration of 500ppm, it showed 100% anti-
bacterial effect for all three bacteria.
At 300ppm, the anti-bacteria effect was in the order of
Staphylococcus aureus > Escherichia coli >
Streptococcus Pyogenes.
Chitosan derivative CsMa showed anti-bacterial effect
against Streptococcus Pyogenes of 98.3%,
Staphylococcus aureus of 1004%, and Escherichia coli
of 96.7% at supply concentration of 300ppm, and at
supply concentration of 500ppm, it showed 100% anti-
bacterial effect for all three bacteria.
At 300ppm, the anti-bacteria effect was in the order of
Staphylococcus aureus > Streptococcus Pyogenes >
Escherichia coli.
The anti-bacteria effect shown in chitosan and
chitosan derivatives is thought to be from having Poly
cationic amine, which is very rare among many
Biopolymers existing on earth, and it was reported that
the degree of freedom of microorganism is confined to
restrain proliferation by formation of ion combination
with huge anion molecule such as Sialic acid and
phosphatide existing in the cell wall of microorganism.
Source: Engineering Master’s Thesis, Graduate School of
Industry, Seoul National University of Technology / 2010
Moisture keeping ability of chitosan
Functional characteristics of Chitin and Chitosan inside
digestive canal
1. Water holding capacity of Chitin and Chitosan
As a result of measuring the water holding capacity of Chitin
and Chitosan by changing the soaking temperature to 3℃, 25℃,
and 37℃, the water holding capacity increased gradually as the
soaking temperature increased.
3). When you look at each sample according to temperature, at
3℃, Chitin showed 6.6~9.9g water/g dry material and chitosan
showed 7.9~13.5g water/g dry material, and in general,
chitosan showed higher water holding capacity than chitin.
Source: Master’s degree application thesis, Graduate School,
Ewha Woman’s University / 1993
Adsorption of chitosan
As a result of preparing and interpreting adsorption
isotherm to compare the Bile acid adsorption
characteristics, Langmuir formula association constants
were 1.44 for chitosan, 0.62 for chitin, and 0.01 for
cellulose, Freundlich formula K values were 25.7, 20.0,
and 16.2 respectively, and 1/n values were 0.96, 1.03,
and 1.12. From the above result, bile acid adsorption
capacity was the biggest for chitosan and chitin and
cellulose followed.
Source: Master’s degree application thesis, Graduate School,
Ewha Woman’s University / 1993
Heavy metal adsorption and removal of
chitosan – at each temperature
When you look at the adsorption removal of heavy metal for
each material, lead showed 91.5% at 30℃, 94.0% at 40℃,
and 96.8% at 50℃, cadmium showed 95.8% at 30℃,
97.7% at 40℃, and 98.0% at 50℃, mercury showed
97.4% at 30℃, 97.4% at 40℃, and 97.4% at 50℃,
manganese showed 70.7% at 30℃, 81.3% at 40℃, and
88.6% at 50℃, and zinc showed 95.8% at 30℃, 96.9% at
40℃, and 98.7% at 50℃.
Source: Master’s degree application thesis, Graduate School,
Sungsin Woman’s University / 2001
Heavy metal adsorption and
removal of chitosan – at each hour
Source: Master’s degree application thesis, Graduate School,
Sungsin Woman’s University / 2001
Anion adsorption ability of chitosan
Chitosan acts as a huge cationic molecule in solution state,
so it has the capacity to absorb various anion state
molecule or material
Moisture and fat adsorption ability of chitosan
The adsorption abilities for moisture and fat of M.W.
24kDa chitosan were 1094.5~1217.9% and
649.9~659.3% respectively and M.W. 4kDa chitosan
having 374.4~424.5% and 307.0~338.8%. Degrees of
Deacetylation (D.D.) of 70% chitosan were
374.4~1094.5% and 307.0~649.9% respectively, D.D. of
85% chitosan were 376.9~1153.9% and 333.8~652.9%,
and D.D. of 92% chitosan were 424.5~1217.9% and
338.8~659.3% respectively.
Chitosan with relatively bigger molecular weight and higher
degree of deacetylation showed to have higher adsorption
ability for moisture and fat.
Source: Master’s degree application thesis, Graduate School,
Bookyung University / 2002
