Specification 1.05454.0500 BRILA (Brilliant-green bile Lactose) broth acc. ISO 4831, ISO 4832 and FDA-BAM Specification ‘Appearance (cleamess) lear ‘Appearance (colour) green pHvalue (25 °C) 70-74 ‘Typical composition (lite: Peptone from meat 10.0; Lactose 10.0; Ox bile dried 20.0; Britant green 0.0138. ‘Growth promotion test in accordance with the current version of DIN EN ISO 11133, Specification inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)) Inoculum on reference medium (Escherichia ‘oli ATCC 8739 (WDCM 00012) Inoculum on reference medium (Citrobacter ‘roundii ATCC 43864 (WDCM 00006) Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WOCM 00009)) Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WOCM 00087)) Inoculum on reference medium (Bacilus cereus ATCC 11778 (WOCM 00001) Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WOCM (00082)) Geowth (Escherichia coli ATCC 25822 (woeM 00013) {Growth (Escherichia coli ATCC 8739 (WOCM 00012)) Growth (Citobacter roundii ATCC 43864 (woeM 09006) Growth (Enterococcus faecalis ATCC 19433, (won 09003) {Growth (Enterococcus faecalis ATCC 29212 (woe 00087) Growth (Bacilus cereus ATCC 11778 (WOCM (00001)) Growth (Staphylococcus aureus ATCC 6538 (woe 09032) Gas (Escherichia coll ATCC 25922 (WOCM 00013)) Gas (Escherichia coli ATCC 8739 (WDCM 00012)» Gas (Citrobacter freundi ATCC 43864 (wc 09006), Gas (Enterococcus faecalis ATCC 19433, (wc 09008) 10-100 10-100 10-100 210 210 2 toto" 210 para iniiton paral inition total inhibition total inhibition Merck KGaA, Frankfurter Strafe 250, 64293 Darmstadt (Germany): +49 6181 72-0 EMD Millipore Corporation -a subsidiary of Merck KGaA, Darmstadt, Germany 290 Concord Road, Bieri, MA 01821, USA, Phone: (978) 715-4321 Page 1 of 2Specification 1.05454.0500 BRILA (Brilliant-green bile Lactose) broth acc. ISO 4831, ISO 4832 and FDA-BAM Gas (Enterococcus fescalis ATCC 20712 (woe 00087) Gas (acilus cereus ATCC 11778(WOCM —- ‘00001)) Gas (Staphylococcus aureus ATCC 6538 (woe 09032)) Growth at 44 °C (Escherichia col ATCC + 28022 (DCM 00013)) Growth at 44 °C (Escherichia coli ATCO 8739 (woe 09012) Gas at 44 °C (Escherichia coll ATCC 25022 + (woeM 09013), Gas at 44 °C (Escherichia coll ATCC 8738 (weM 09012) Incubation E.col and C.roundi 24 2 2 hours at 30 1 °C aerobic. ‘ther teststrane: 48 2 hours at 30 = 1°C aerobic, Ecol additionally 24 2 hours at 44 + 1 °C aerobic. Reference medium: Tryptic Soy Agar Stefanie Fischer ‘Response lborlany manage qual conial This document has been produced electronically ands valid without a signature, Merck KGaA, Frankfurter Strae 250, 64293 Darmstadt (Germany): +49 6151 72.0 Page 2 of 2 EMD Millipore Corporation - a subsidiary of Merck KGaA, Darmstadt, Germany 200 Concord Road, Bilerica, MA 01821, USA, Phone: (978) 715-4321