‫اسئلة امتحان الهيئة ميكروبيولوجى ومختبرات‬

(1) the best anti coagulant in blood culture is?

*sodium oxalate
*sodium citrates
*sodium sulphate@

(2) all
the following samples are stained using gram stain
except?
*water culture
*sputum
*stool@

(3) the percentage of sodium chloride in agar culture is?

*0.058%
*58.0%
*0.58%

(4) agar is characterized by all the following except?

*freezing point is 42c &
melting point is 100c
*nutritive
*un-nutritive@

(5) the normal value of (Na) in serum is?

*135-145 mg/dl
*135-145 mmol/dl
*135-145 mmol/l@

(6) gram stain starts with?

*iodine
*crystal green
*methyl violet /@

(7) the causative of toxoplasma is?

*flees
*dogs
*cats@

(8) gram negative samples are stained with?

*yellow color
*blue color
*red color@

(9) the color of cholera when cultured on TCBS?

*blue
*green
*yellow@

(10) the diameter of micro pore filter used in bacteria filtration

is?
*0.033microns
*2.2microns
*.22microns@

(11) leishmania is transported by?

*mosquitoes
*pugs
*sand fly@

(12) to
investigate one sample containing
floro-carbon we use which microscope?
*light microscope
*brilliant microscope
*florescent microscope@

(13) virus is similar to live organisms in?

*growth
*movement
*reproduction@

(14) the largest resolution of the microscope is?

*0.02microns
*2.0microns
*0.2microns@

(15) which of the following is liver function test?

*liver enzymes
*serum + urin billrubin
*all of the previous@

(16) which
of the following causing
enlargement of RBCs?
*reticulocytes
*leishmania
*plasmodium@

(17) the best sample for the culture of children paralysis virus
is?
*anal swab
*blood culture
*stool culture@

(18) dry air oven is used to sterilize?

*water
*culture media
*sand@

(19) the
diabetic patient is going to comma when blood
glucose is?
*120 mg/dl
*160 mg/dl
*less than 50mg/dl@

(20) to
differentiate between the two streptococci group which
enzyme is used?
*coagulase
*kinase
*catalase@

(21) the microscope which used in investigate syphilis is?

*light microscope
*ultra-violet microscope
*dark field microscope@

(22) the
antibiotic disc which differentiate between group A
streptococci and other groups is?
*neomycin
*tetracycline
*bacitracin@

(23) all of the following is true for salmonella except?

*motile
*produces H2S
*oxidase positive@

(24) amylase value is high in the following disease?

*salivary glands
*pancreas diseases
*all of the previous@

(25) one jaundice patient has yellow skin, his billrubin is

*2.5mg/dl
*1.2mg/dl
*5.0mg/dl@

(26) acute diabetic patient has?

*blood sugar more than 150mg/dl
*blood sugar more than 180mg/dl
*glucose & acetone in urine@

(27) the normal value of billrubin is?

*0.2-2.0mg/dl
*less than 2.0mg/dl
*less than 1.0mg/dl@

(28) diabetic patient has one of the following symptoms?

*acidosis
*alkalosis
*dryness@
(29) insulin is regulating blood sugar by?
*increase the influx of glucose into cells
*activate glycogensis
*all the previous@

(30) the nutritive substance in culture media is?

*sugar
*starch
*agar@

(31) the best media for urin culture is?

*blood agar
*chocolate agar
*cled agar@

(32) the sterilization of autoclave is?

*85c for 30min
*150c for 30min
*121c for 15min@

(33) shigella soni colored in maconkey & EMB?

*colorless
*red
*pink@

(34) the oil emertion lens is

*10
*40
*100@

(35) the dwarf worm is?

*schistosoma
*tenia saginata
*H.nana

(36) all of the following are present in gram stain except?

*safranine
*iodine
*malachite green@

(37) the diagnostic stage of amoeba is?

*cyst
*trophozoite
*all previous@

(38) to check the intestinal efficacy the following test is done?

*pepsin
*lipase
*stool fats@

(39) the worm that causes blood in urin?

*ascaris
*fasciola
*schistosoma haematobium@

(40) the significant count of bacteria to be inflammation is?

*less than 10^5
*more than 10^3
*more than 10^5@

(41) to
investigate gram stained samples we use the following
microscope?
*double phase microscope
*florescent microscope
*light microscope@

(42) the most important buffer system in blood is?

*acetic acid system
*hemoglobin system
*bicarbonates system@

(43) all the following bacteria are interobacter except?

*E.coli
*proteus
*non of the previous @

in the microscope when using objective lens 10 &

(44)
eye lens 10 thee magnification power of the microscope is?
*10
*1000
*100@

(45) all
the following parameters affecting gram staning
except?
*use H2SO4
*add absolute alcohol after washing
*delaying the dryness of the slide@

(46) glycogen is stored in?

*liver
*muscles
*all the previous@
(47) malaria chizonts are present in?
*reticulo-endothelial
*leukocyte
*RBCs@

(48) comma of diabetic patient shows?

*glucose grater than 200mg/dl
*glucose less than 200mg/dl
*glucose grater than 500mg/dl@

(49) blood donor selection in KSA are all of the following

except?
*Hb% 12.5-16
*free from syphilis
*one year after delivery@

(50) solidifying agent in culture media is?

*wax
*starch
*agar@
(51) agar
which used as a solidifying agent in culture media
concentration is?
*5-9%
*4-9%
*1-2%@

(52) to sterilize culture media we use?

*boiling
*hot oven
*autoclave@

(53) bone matrix can also called?

*vascular tissue
*fibrous tissue
*osteon@

(54) unidirectional movement of WBCs directly to its target is?

*sliding
*phagocytes
*chemo taxis@

(55) when
focusing a stained smear under oil immersion field
the magnification is?
*10
*40
*100@

(56) in myocardial infection?

*level of LHD high
*level of GOT high
*level of CK-MB high@
(57) variation in RBCs size?
*microcytosis
*macrocytosis
*aniso cytosis@

(58) pretranfusion
test that is performed using the patient red
cells and donor plasma?
*Anti body screen
*major x-matching
*minor x-matching2

(59) serum LDH is elevated in all the following except?

*skeletal disease
*cardiac/ hepatic diseases
*renal disease@

(60) elevated sodium &chloride is seen in?

*shock
*diabetic acidosis
*severe dehydration@

(61) which test is better to diagnose chronic bile duct?

*total bilirubin
*S-GOT
*ALP@

(62) generallydiagnosed by recovery & identification of

typical larva in stool?
*hook worms
*t.trichura
*s.stercoralis@
(63) enlarged RBCs are common in?
*p.malaria
*p.falciparum
*p.vivax@

(64) whichof the following is not laying eggs in small

intestine?
*hook worm
*t.saginata
*pin worm@

(65) blood sample is used to diagnose?

*C.tetani
*C.diphteria
*non from them@
(66) how
much water should we add to 500ml of a solution of
10% of NAOH to bring it to 7.5%?
*666
*250
*166@

(67) while using the pregnancy test we are measuring?

*total HCG
*beta HCG &LH
*beta HCG@

(68) with age the renal threshold for glucose?

*increase
*decrease
*does not change@

(69) calibrator sera are?

*secondary standards
*internal standards
*primary standards@

(70) a buffer made of?

*a strong acid + a strong salt
*a weak acid + a weak salt
*a weak acid + a strong salt@

(71) a standard micro plates in ELISA test has?

*98 wells
*94 wells
*96 wells@

(72) the enzyme in ELISA testing is present in the?

*buffer
*micro plate
*conjugate@

(73) antigen antibody complex are?

*weakly bound
*no bounds
*strongly bound@

(74) washing
must be done in all heterogeneous ELISA
technique because?
*increase the specificity
*increase the sensitivity
*it removes the excess binding@
(75) the label in ELISA tests is?
*radio active substance
*antibody
*enzyme@

(76) the difference between plasma & serum is that plasma?

*does not contain fibrinogen
*has more water
*contains fibrinogen@

(77) five
ml of colored solution has an absorbance of .500nm
The absorbance of 10ml of the same solution is?
*1.000nm
*0.250nm
*0.500nm@

(78) plasmaor serum should be separated at the earliest time

for estimation of glucose because?
*glucose value increases with time
*lyses of blood will occur
*glucose value decreases with time@

(79) sensitivity & specify are?

*directly related
*they mean the same
*non of above@

(80) a dichromatic analysis is carried to increase?

*linearity
*specify
*sensitivity@

(81) causes of high serum bilirubin are?

*overload on liver
*haemolysis
*all of the previous@

(82) polio myeletis is transmitted through?

*skin
*respiration
*feco-oral@

(83) malaria does not grow in?

*EDTA blood
*heparin zed blood
*plasma@
(84) serious that causes food poisoning?
*staph albus
*salmonella typhi
*salmonella enteritidis@

(85) hemophiliaman married to normal woman the incidence

of his children is?
*carrier male
*diseased female
*carrier female@

(86) which of the following causes UTI & INDOL positive?

*klebsiella
*staphylococci
*E.coli@

87) blood transfusion can transmit?

*HIV
*CMV
*all of the previous@

(88) ADH is secreted from?

*thyroid gland
*anterior pituitary
*posterior pituitary@

(89) one of the following enzymes is effected by hemolysis?

*SGOT
*SGPT
*LDH@

(90) HbA1C of diabetic patient is important for?

*he has to come fasting
*short term follow up
*long term follow up@

(91) for glucose tolerance test?

*collect 5 blood samples only
*collect 5 urin samples only
*collect 5 blood samples + 5 urin samples@

(92) one
of the following heart enzymes is measured
after 4-8hr of chest pain?
*GOT
*LDH
*CPK@
(93) light effects one of the following?
*glucose
*urea
*billrubin@

(94) one of the following is specific diagnostic liver enzyme?

*GOT
*LDH
*GPT@

(95) one of the following is important before anesthesia?

*alkaline phosphates
*acidic phosphates
*pseudocholine esterase@

(96) acid phosphates is?

*heart enzyme
*liver enzyme
*prostatic enzyme@

(97) In uric acid estimation?

*its affected by carbohydrate meal
*no need for fasting
*the patient has to come fasting@

(98) all of the following are affected by meal except?

*glucose
*albumin
*creatinine@

(99) Na is the main?

*intra cellular anion
*intra cellular cation
*extra cellular cation@

(100) one
of the following electrolytes is effected by
hemolysis?
*Ca
*Mg
*k@

(101) the best kidney function test?

*urea
*total protein
*creatinine clearance@
(102) for GGT in adults the dose of glucose is?
*50gram
*100gram
*75gram@

(103) for GGT in children the dose of glucose is?

*30mg
*15gram
*30gram@

(104) random blood glucose gives an idea?

*to justify blood glucose
*fasting patient
*blood glucose in urgent cases@

(105) immunoglobulin is?

*B globulin
*Alfa globulin
*Gamma globulin@

(106) exogenous triglecride is carried on?

*VLDL
*HDL
*chylomicron@

(107) endogenous triglecride is carried on?

*LDL
*HDL
*VLDL@
(108) harmful cholesterol is carried on?
*HDL
*VLDL
*LDL@

(109) useful cholesterol is carried on?

*chylomicron
*LDL
*HDL@
(110) for lipid investigation patient has to fast?
*4-6hr
*6-8hr
*12-14hr@
(111) to measure G6PD we use?
*plasma
*serum
*whole blood on EDTA@
(112) amoeba moves by?
*cilia
*flagella
*pseudopodia@

(113) one of the following is capsulated bacteria?

*streptococci
*E.coli
*klebsiella@

4.a semen specimen, contain sugar source of the spermatozoa energy is:
§ Glucose
§ Mannose
§ Glactose
§ Fructose
§ Sucrose

5.CPD_A anticoagulant used to store blood for:

§ 35 days
§ 45 days
§ 5 days
§ 15 days
§ Non of the above

6During reaction of two chemical substances , the color produced asses by

§ Spectrophotometer
§ Flame photometer

7.glycolysis done in :
§ Nucleus
§ Endoplasmic reticulum
§ Mitochondrion
§ Cytoplasm
§ Non of the above

8.one of the above not present in Diabetic mellitus coma :

§ Hyperglycemia
§ Hypercholesterolemia
§ High number of ketene in urine
§ Non of the above
9.in which substance give blood glucose when hydrolysis :
§ Muscle glycogen
§ Liver glycogen
§ Heart glycogen
§ Unsaturated fat
§ Triglyceride

10.in Anti human globulin test do wash of RBC because all serum contain :
§ Albumin
§ a_glubulin
§ b_ globulin
§ fibrinogen
§ Immunoglobulin

11. the most source of ATP in cell is :

§ Mitochondria
§ Cytoplasm
§ Nucleus
§ Cell wall

12 . H Ag present in :
§ Pilli
§ Capsule
§ Flagella
§ Cell membrane

13. urea is final product of catabolism of:

§ amino acid
§ triglyceride
§ cholesterol
§ polysaccharide

14.Which of White blood cell give immunoglobulin :

§ lymphocyte
§ Neutrophil
§ Basophile
§ Monocyte
§ Eosinophil
15.Blood of Microfilaria infection diagnosis with :
§ Wuchereia bancofti

16.one of the immunoglobulin present in trace amount in serum § IgG

§ IgE
§ IgM
§ IgD
§ IgA

17.gram _ve bacteria colonies color appear :

§ Dark purple
§ Pale to dark red
§ Orange

18. alkali burn treatment by neutralization with :

§ Sodium bicarbonate powder
§ Boric acid
§ Acetic Acid
§ Cold water
§ Hot water

19.reason of false positive ABO in compatibility:

§ Cold agglutination

20. selective and Differential medium of entero pathogen is:

§ Chocolate agar
§ Blood agar
§ DCA medium
§ Meat extract agar
§ Non of the above

21.in O blood group the Ab in serum is :

§ Anti A1B
§ Anti A
§ Anti B
§ Non of the above
§ All of the above

22.Neutrophil is acommn White blood cell present in blood and the percentage of
presence is :
§ 90%
§ 15%
§ 75%

23.The leukocyte that involve in Adaptive and Acquired immunity

§ Lymphocyte
§ Monocyte
§ Neutrophil
§ Basophil
§ Eosinophil

24. one of these is prokaryotic cell:

§ Fungi
§ Bacteria
§ Entameba histolytica
§ All of the above
§ Non of the above
25.Most sever (serious) malaria infection caused by:
§ Plasmodium faliceparm
§ Plasmodium Malaria
§ Plasmodium Ovale
§ Plasmodium Vivax
§ All of the above
26.To detect pH from solution use :
§ pH meter
§ Indicator
§ Micrometer

27. one of these organism multiply intracellular parasite:

§ Gardia lamblia
§ Entameba histolytica
§ E. coli
§ Tryponosoma Cruzi
§ Toxoplasma gondii
28.gram-ve rod non motile :
§ Salmonella
§ Yersini pestis
§ Shigella species
§ Vibrio cholera

29. Leukocyte responsible for cellular immunity:

§ T_ lymphocyte
§ B- lymphocyte
§ Monocyte
§ Basophile
§ Eosinophil

30.one of these org gram +ve cocci arranged in pairs :

§ Staphylococci
§ Streptococci
§ Meningococci
§ entrococci

31. One is not a Romanowsky stain

§ Fields stain
§ Gram stain
§ Geimsa stain
§ Leishman stain

32. Hydrolysis of sucrose result:

§ Only glucose
§ Galactose
§ Fructose + glucose
§ Glucose + Glucose
§ Maltose + Glucose

32.method used to detect horomone in the laboratory :

§ ELIZA

33. one of the following has highest conc. Of cholesterol:

§ Chylomicron
§ LDL
§ HDL
§ VLDL
§ Apolipoprtein

34. oxytocine hormone function :

§ Increase Oogenesis
§ Stimulate contraction of uterus
§ Increase basal metabolic

35. Iodine stain of Entamoeba cyst used to detect (diagnose):

§ Chromatoidal bars
§ Cell wall
§ Nuclei

36. function of Cortisol :

§ Increase Oogenesis
§ Stimulate contraction of uterus
§ Increase basal metabolic

37.infective stage of Ascaris lumbricodes :

§ Larva
§ Cercaria
§ Egg
§ Cyst
§ Metacercaria

38.substance used in catalyse reaction :

H2O2

39. Normal range of erythrocyte

- 4,5- 6,5X 10^9

40.one of these leukocyte have 2_5 lobes in nucleus :

§ Neutrophil
§ Basophil
§ Monocyte
§ Lymphocyte
§ Eosinophil

41. one of these cell the largest leukocyte cell:

§ Neutrophil
§ Basophil
§ Monocyte
§ Lymphocyte
§ Eosinophil

42.Alkline phosphates is the important enzyme to detect function in :

§ Liver
§ Bone
§ Liver and bone
§ Non of the above
43.one of the following anticoagulant used for blood glucose :
§ EDTA
§ Heparin
§ Sodium oxalate
§ Florida Oxalate
§ Sodium citrate

44.AST important enzyme to detect abnormality in:

§ Liver disease
§ Heart disease
§ Liver and heart disease
§ Non of the above

45. the defect of thalassemia occur in:

§ Cycle of heme
§ Glubin chain
§ acute flammable
§ Toxic
§ Corrosive
§ Explosive