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Laboratory Exercise 1

The document is a laboratory exercise on microscopy that includes questions to test understanding of microscope usage and principles. It asks students to describe how moving a specimen affects its image, and that the image is inverted and enlarged. It also asks about the relative fields of view of low and high power objectives, and has students calculate total magnification and field of view given magnification specifications. Finally, it addresses why colored dyes are used to view cells, and has students draw specimens they viewed under the microscope.

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Camille Santos
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68 views

Laboratory Exercise 1

The document is a laboratory exercise on microscopy that includes questions to test understanding of microscope usage and principles. It asks students to describe how moving a specimen affects its image, and that the image is inverted and enlarged. It also asks about the relative fields of view of low and high power objectives, and has students calculate total magnification and field of view given magnification specifications. Finally, it addresses why colored dyes are used to view cells, and has students draw specimens they viewed under the microscope.

Uploaded by

Camille Santos
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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University of Santo Tomas

Senior High School – Biology


Laboratory Exercise 1

Microscopy
A. Answer the following question. Essay question should be answered briefly (maximum of 5
sentences)
1. If you move the specimen to the right, what will be the direction of the image in the ocular?
Why?
The image that you will see will appear to be upside-down and moves to the left. This is
because the lenses that are in the microscope act like the mirrors causing the image that you see in
the ocular lens to become inverted.

2. Describe the image in the ocular.


The image seen in the ocular is the specimen in backward and inverted view. The image is also
enlarged by the ocular. Thus, what you see in the ocular is the virtual image of the specimen.

3. Which has the greater field of view, the low power objective or the high power objective? Why?
The low power objective has the greater field of view because it is not zoomed into the center
of the specimen like the high power objective. The LPO shows more of the object because it is
farther while the HPO has the closer view of the cell structure.

4. Compute the following:


a. What is the total magnification of the letter “e” if the ocular and the objective has a magnification
of 10x and 40x, respectively?
𝑇𝑜𝑡𝑎𝑙 𝑀𝑎𝑔𝑛𝑖𝑓𝑖𝑐𝑎𝑡𝑖𝑜𝑛 = 𝑂𝑏𝑗𝑒𝑐𝑡𝑖𝑣𝑒 𝑃𝑜𝑤𝑒𝑟 × 𝑂𝑐𝑢𝑙𝑎𝑟 𝑃𝑜𝑤𝑒𝑟
𝑇𝑜𝑡𝑎𝑙 𝑀𝑎𝑔𝑛𝑖𝑓𝑖𝑐𝑎𝑡𝑖𝑜𝑛 = 40𝑥 × 10𝑥
𝑇𝑜𝑡𝑎𝑙 𝑀𝑎𝑔𝑛𝑖𝑓𝑖𝑐𝑎𝑡𝑖𝑜𝑛 = 400𝑥
The total magnification of the letter “e” would be 400x because it uses high power objective
which has 10x ocular lens and 40x objective lens.

b. What is the field of view if the ocular has a 10x magnification with 20mm number and a total
magnification of 100x?
𝑓𝑖𝑒𝑙𝑑 𝑛𝑢𝑚𝑏𝑒𝑟
𝐹𝑂𝑉 =
𝑚𝑎𝑔𝑛𝑖𝑓𝑖𝑐𝑎𝑡𝑖𝑜𝑛 𝑛𝑢𝑚𝑏𝑒𝑟
20𝑚𝑚
𝐹𝑂𝑉 =
100𝑥
𝐹𝑂𝑉 = 0.2𝑚𝑚
1000𝑚𝑚
0.2𝑚𝑚 × = 200 𝜇𝑚
1𝑚𝑚

𝐹𝑂𝑉 = 200 𝜇𝑚
The field of view would be 200 micrometers because it has 20mm field number and 100x total
magnification.
5. Are all the threads focused at the same time? Why?
No, because the same as taking a photo from a camera, the subject can’t all be focused at once.
The amount of thread that will be focused depends on its magnification (4x, 10x or 40x). A
microscope with a greater field can’t focus all three threads at the same time. Same with lenses
that have higher magnifications.

6. Why do we need to use a colored dye when viewing the cell/s?


We used colored dye to view cells better as they highlight each cell structure and it can also
help us see the magnified cellular components more clearly than just plain colorless ones/cells.

B. Draw the specimens viewed.

LPO HPO

Letter E

Hair and Thread

Onion Cells

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