Users Guide Vol.

AU400 e ®/AU400 ®
Chemistry Analyzer

For In Vitro Diagnostic Use

BM400V2 AB
JUNE 2013
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
AU400e® /AU400® Chemistry Analyzer
User’s Guide
PN BM400V2AB (JUNE 2013)

Copyright © 2013 Beckman Coulter, Inc.

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Table of Contents Volume 2
Table of Contents Volume 2........................................................................................iii

Chapter F Maintenance
1. Maintenance Schedules and Preparation........................................................ F-3
2. Daily Maintenance.............................................................................................. F-9
2.1 Inspect Sample and Reagent Syringes for Leaks.................................................................. F-10
2.2 Check the Wash Solution Rolling Pump for Leaks................................................................. F-11
2.3 Inspect the Concentrated Wash Solution Level...................................................................... F-12
2.4 Inspect and Clean Sample Probe, Reagent Probe, and Mix Bars.......................................... F-13
2.5 Inspect the Stability of the Upper Cover............................................................................... F-13a
2.6 Inspect the Printer and Paper................................................................................................. F-14
2.7 Prepare for a Sample Probe Wash......................................................................................... F-15
2.8 Change DI water in the Pre-dilution Bottle............................................................................. F-16
3. Weekly Maintenance........................................................................................ F-17
3.1 Perform a W2 (Washes Cuvettes, Mix Bars, Reagent Probes, and Waste Lines)................. F-18
3.2 Perform a Photocal (after the W2).......................................................................................... F-20
3.3 Perform a Photometer Check................................................................................................. F-21
3.4 Wash the Sample Pre-dilution Bottle...................................................................................... F-22
4. Monthly Maintenance....................................................................................... F-23
4.1 Clean the Sample Probe and Reagent Probe Wash Wells.................................................... F-24
4.2 Clean the Mix Bar Wash Wells............................................................................................... F-25
4.3 Clean the Wash Nozzle Unit, Deionized-Water Tank and Filter, and Sample Probe Filter..... F-26
5. Maintenance Required Every Three Months.................................................. F-33
5.1 Replace the Wash Solution Rolling Tube............................................................................... F-34
5.2 Clean Air Filters...................................................................................................................... F-35
6. As Needed Maintenance.................................................................................. F-37
6.1 Replace the Sample and Reagent Probes............................................................................. F-38
6.2 Replace Mix Bars.................................................................................................................... F-40
6.3 Replace the Wash Nozzle Joint Tubes................................................................................... F-41
6.4 Replace Sample and Reagent Syringes................................................................................. F-44
6.5 Replace the Photometer Lamp............................................................................................... F-46
6.6 Clean or Replace Individual Cuvettes.................................................................................... F-48
6.7 Clean the Cuvettes and the Cuvette Wheel (Part of 6 month PM)......................................... F-49
6.7a Clean the Cuvettes and Cuvette Wheel after a Cuvette Wheel Flood.................................. F-50a
6.8 Perform a W1 Procedure........................................................................................................ F-51
6.9 Clean Belts and Rack Feed areas.......................................................................................... F-52
6.10 Clean the Wash Nozzle Unit................................................................................................... F-53
6.11 Clean or Replace the Sample Probe Filter............................................................................. F-56
6.12 Clean the Deionized-Water Tank............................................................................................ F-58
6.13 Clean or Replace the Deionized-Water Filter......................................................................... F-60
6.14 Clean or Replace the Static Discharge Brushes.................................................................... F-62
7. ISE Maintenance .............................................................................................. F-63
7.1 Daily ISE Maintenance........................................................................................................... F-64
7.1.1 Inspect the ISE Reagent (Buffer) Syringe for Leaks................................................... F-65
7.1.2 ISE Cleaning............................................................................................................... F-66
7.2 Weekly ISE Maintenance........................................................................................................ F-68
7.2.1 Perform a Selectivity Check for the Na/K Electrodes................................................. F-69
7.3 Maintenance Every Two Weeks............................................................................................. F-70
7.3.1 Clean the mix bars, liquid level sensors, sample pot and sample pot tubing. ........... F-71
7.4 ISE Maintenance Required Every Three Months................................................................... F-75
7.4.1 Replace the Mixture and Mid-Standard Pump Roller Tubing..................................... F-76
7.4.2 Replace Valve Tubing................................................................................................. F-78
7.5 As Needed ISE Maintenance................................................................................................. F-80
7.5.1 Replace the Reference Electrode............................................................................... F-81
7.5.2 Add Reference Electrode Solution............................................................................. F-83
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AU400e/AU400 User's Guide iii
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 7.5.3 Replace Reagents...................................................................................................... F-84
7.5.4 Replace the Na, K, or Cl Electrode............................................................................. F-86
7.5.5 ISE Cleaning Procedure............................................................................................. F-88
7.5.6 Replace the reagent buffer syringe............................................................................. F-88

Chapter G Error Flags

1. Checking Results...............................................................................................G-4
2. Data Error Flags.................................................................................................G-5
2.1 Troubleshooting for Data Flags ?, @, $, D, F, G, !..................................................................G-18
3. Non-Fatal and Fatal Errors.................................................................................G-21
3.1 Non Fatal Errors (Yellow)........................................................................................................G-22
3.2 Fatal Errors (Red)...................................................................................................................G-25

Chapter H Troubleshooting
To Begin Troubleshooting, Answer the Following Questions............................................................H-3
1. Troubleshooting Using On-Line Help...............................................................H-5
2. Troubleshooting the Analyzer...........................................................................H-7
2.1 Data Problems..........................................................................................................................H-8
2.1.1 Checking Abnormal Data in the Software Screens.......................................................H-9
2.1.2 Troubleshooting Software........................................................................................... H-11
2.1.3 Troubleshooting Reagents and Samples....................................................................H-13
2.1.4 Troubleshooting Mechanical Problems.......................................................................H-16
2.2 System Problems....................................................................................................................H-21
2.3 Data Processor Problems.......................................................................................................H-25
2.4 Recovering from an Emergency Stop or Power Loss.............................................................H-28
2.5 Recovering from a Cuvette Wheel Overflow..........................................................................H-29
2.5.1 What Causes a Overflow?..........................................................................................H-29
2.5.2 Recognizing a Overflow..............................................................................................H-29
2.5.3 Recovering from a Overflow......................................................................................H-30
2.5.4 After the Overflow Problem Is Fixed...........................................................................H-30
3. Troubleshooting the ISE .................................................................................H-31
To Begin Troubleshooting the ISE, Answer the Following Questions.....................................H-32
3.1 ISE Sample Requirements...................................................................................................H-34
3.2 Dispensing System................................................................................................................H-35
3.2.1 Sample Probe and ISE Reagent Syringe...................................................................H-36
3.2.2 Pump Tubing:..............................................................................................................H-37
3.2.3 Mix Bar........................................................................................................................H-38
3.2.4 Sample Pot.................................................................................................................H-39
3.2.5 Pinch Valve Tubing.....................................................................................................H-40
3.3 Measuring Components........................................................................................................H-41
3.3.1 O-rings........................................................................................................................H-42
3.3.2 Electrodes...................................................................................................................H-43
3.3.3 Mixture Pump Tubing..................................................................................................H-45
3.3.4 Thermistor...................................................................................................................H-46
3.3.5 Flowcell Block.............................................................................................................H-47
3.4 Calibration Errors...................................................................................................................H-48
3.5 Selectivity Check....................................................................................................................H-50
3.6 Sequential Sample Measure................................................................................................H-51
3.6.1 Shifts & Trends...........................................................................................................H-52
3.6.2 How to Check Reagent Integrity.................................................................................H-54

Chapter I Specifications
1. System Operation During Analysis................................................................... I-3
Operation Process..................................................................................................................... I-3
Operation Modes....................................................................................................................... I-4
2. Analyzer Hardware Configuration..................................................................... I-5
Operation Switches................................................................................................................... I-5
Rack Feeder Unit....................................................................................................................... I-7
Sample Probe and Reagent Probe Units.................................................................................. I-9
The Mix Unit............................................................................................................................. I-10
The Wash Nozzle Unit..............................................................................................................I-11

iv
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 The STAT Table....................................................................................................................... I-12
Sample Syringe and Reagent Syringes................................................................................... I-14
Rolling Pump Unit.................................................................................................................... I-15
Reagent Refrigeration Unit...................................................................................................... I-16
The Incubator.......................................................................................................................... I-18
The Photometer Unit............................................................................................................... I-19
Tank Storage........................................................................................................................... I-20
Breakers and Fuse.................................................................................................................. I-21
The ISE Unit ........................................................................................................................... I-22
The ISE Reagent Syringe ....................................................................................................... I-23
The ISE Reagent Bottles......................................................................................................... I-24
3. System Connections......................................................................................... I-25
4. Major System Specifications............................................................................ I-26
Specifications Related to System Installation.......................................................................... I-26
Sampling Specifications.......................................................................................................... I-27
Reaction Unit Specifications.................................................................................................... I-28
Photometer Unit Specifications............................................................................................... I-29
Data Processor Unit Specifications......................................................................................... I-29
Analysis Processing Specifications......................................................................................... I-29
Input/Output Specifications...................................................................................................... I-30
ISE Unit Specifications............................................................................................................ I-34
5. Calculations....................................................................................................... I-35
5.1 Reagent Blank (Zero Adjustment)........................................................................................... I-35
5.2 Endpoint Assay........................................................................................................................ I-37
5.3 Rate Assay............................................................................................................................... I-39
5.4 Fixed Point Assay.................................................................................................................... I-40
5.5 Sample Blank........................................................................................................................... I-41
5.6 LIH........................................................................................................................................... I-42
6. Quality Control.................................................................................................. I-43
7. AU400 Terminology........................................................................................... I-49

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AU400e/AU400 User's Guide v
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vi
Automated Chemistry Analyzer
AU400e/AU400 User's Guide
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 Chapter F
Maintenance

Introduction
This chapter includes detailed information about the maintenance required for each system
component. To ensure data is accurate, perform maintenance on schedule, and keep a record on
the maintenance/inspection check lists provided in this chapter.

Caution
Follow laboratory safety guidelines when performing maintenance procedures. Observe all warning and
caution instructions listed in the following procedures. Also, do not place any body parts in the path
of moving equipment, unless you are certain that the analyzer is in Stop or Standby and that diagnostic
functions are not activated.

Chapter F Maintenance
Introduction............................................................................................................... F-1
1. Maintenance Schedules and Preparation........................................................ F-3
2. Daily Maintenance.............................................................................................. F-9
2.1 Inspect Sample and Reagent Syringes for Leaks.................................................................. F-10
2.2 Check the Wash Solution Rolling Pump for Leaks................................................................. F-11
2.3 Inspect the Concentrated Wash Solution Level...................................................................... F-12
2.4 Inspect and Clean Sample Probe, Reagent Probe, and Mix Bars.......................................... F-13
2.5 Inspect the Stability of the Upper Cover............................................................................... F-13a
2.6 Inspect the Printer and Paper................................................................................................. F-14
2.7 Prepare for a Sample Probe Wash......................................................................................... F-15
2.8 Change DI water in the Pre-dilution Bottle............................................................................. F-16
3. Weekly Maintenance........................................................................................ F-17
3.1 Perform a W2 (Washes Cuvettes, Mix Bars, Reagent Probes, and Waste Lines)................. F-18
3.2 Perform a Photocal (after the W2).......................................................................................... F-20
3.3 Perform a Photometer Check................................................................................................. F-21
3.4 Wash the Sample Pre-dilution Bottle...................................................................................... F-22
4. Monthly Maintenance....................................................................................... F-23
4.1 Clean the Sample Probe and Reagent Probe Wash Wells.................................................... F-24
4.2 Clean the Mix Bar Wash Wells............................................................................................... F-25
4.3 Clean the Wash Nozzle Unit, Deionized-Water Tank and Filter, and Sample Probe Filter..... F-26
5. Maintenance Required Every Three Months.................................................. F-33
5.1 Replace the Wash Solution Rolling Tube............................................................................... F-34
5.2 Clean Air Filters...................................................................................................................... F-35
Automated Chemistry Analyzer
AU400/AU400e User's Guide Chapter F - Maintenance F-1
April 14, 2010
 6. As Needed Maintenance.................................................................................. F-37
6.1 Replace the Sample and Reagent Probes............................................................................. F-38
6.2 Replace Mix Bars.................................................................................................................... F-40
6.3 Replace the Wash Nozzle Joint Tubes................................................................................... F-41
6.4 Replace Sample and Reagent Syringes................................................................................. F-44
6.5 Replace the Photometer Lamp (Part of 6 month PM)............................................................ F-46
6.6 Clean or Replace Individual Cuvettes.................................................................................... F-48
6.7 Clean the Cuvettes and the Cuvette Wheel (Part of 6 month PM)......................................... F-49
6.7a Clean the Cuvettes and Cuvette Wheel after a Cuvette Wheel Flood.................................. F-50a
6.8 Perform a W1 Procedure........................................................................................................ F-51
6.9 Clean Belts and Rack Feed areas.......................................................................................... F-52
6.10 Clean the Wash Nozzle Unit................................................................................................... F-53
6.11 Clean or Replace the Sample Probe Filter............................................................................. F-56
6.12 Clean the Deionized-Water Tank............................................................................................ F-58
6.13 Clean or Replace the Deionized-Water Filter......................................................................... F-60
6.14 Clean or Replace the Static Discharge Brushes.................................................................... F-62
7. ISE Maintenance .............................................................................................. F-63
7.1 Daily ISE Maintenance........................................................................................................... F-64
7.1.1 Inspect the ISE Reagent (Buffer) Syringe for Leaks................................................... F-65
7.1.2 ISE Cleaning............................................................................................................... F-66
7.2 Weekly ISE Maintenance........................................................................................................ F-68
7.2.1 Perform a Selectivity Check for the Na/K Electrodes................................................. F-69
7.3 Maintenance Every Two Weeks............................................................................................. F-70
7.3.1 Clean the mix bars, liquid level sensors, sample pot and sample pot tubing. ........... F-71
7.4 ISE Maintenance Required Every Three Months................................................................... F-75
7.4.1 Replace the Mixture and Mid-Standard Pump Roller Tubing..................................... F-76
7.4.2 Replace Valve Tubing................................................................................................. F-78
7.5 As Needed ISE Maintenance................................................................................................. F-80
7.5.1 Replace the Reference Electrode............................................................................... F-81
7.5.2 Add Reference Electrode Solution............................................................................. F-83
7.5.3 Replace Reagents...................................................................................................... F-84
7.5.4 Replace the Na, K, or Cl Electrode............................................................................. F-86
7.5.5 ISE Cleaning Procedure............................................................................................. F-88
7.5.6 Replace the reagent buffer syringe............................................................................. F-88

F-2 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August 29, 2008
1. Maintenance Schedules and Preparation
Periodic Maintenance
• Record on-line maintenance records in [Maintenance], [Periodic Maintenance].
• To display a maintenance schedule, select F5 Grid Display.
• To update the maintenance schedule, place the cursor on a maintenance item and select F5
Execute. The analyzer must be in a standby mode.
• To display the last 10 dates maintenance was performed and the next date due, select F6
History.
• To add additional maintenance items to the Periodic Maintenance schedule, select function
key F7 Entry. Beginning with line 37, scroll to a blank line and click to highlight it. Select
F7 Entry and type in the name of the maintenance item. Select the scheduled interval (days,
weeks, months, or years) from the pull-down menu called “period unit.” Enter a number
in the period option for the assigned time. To add another maintenance item, select the
►button and repeat the previous steps. When all maintenance items are added, select the
close button.
• To obtain a printout of the maintenance records, select F3 Print.

Consumable Management OLP1cons

STANDBY
?
1998/08/03 10:10

Consumable Management

No. Part Changed Used

1. Lamp hours
2. S. Probe times
3. R. Probe times
4. S. Syringe times
5. R. Syringe times
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.

Help Exit Print Change History Entry Delete

ALARMCLEAR

• Track critical components through Consumable Management for timely warranty

replacement.
• Record consumable items on-line in [Maintenance], [Consumable Management].
• Place the cursor on a consumable item to update. The item is highlighted in blue. Select F5
Change. The consumable management window displays. At the prompt “Renewal OK?”
select OK for the changed item. A record of the time period an item is used is then tracked.
• To view a listing of the last 10 times an item was changed and how long the item was used,
select function key F6 History

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-3
May 19, 2006
 • To add additional consumable items to Consumable Management, select F7 Entry.
Beginning with line 14, scroll to a blank line and select it. Select F7 Entry, then type in a
consumable name. Next, select a time unit (days, weeks, months, or years) from the pull-
down menu called “unit.” Enter a number in the lifetime option for the assigned time. To
add another consumable item, select the ► button and repeat the previous steps. After all
consumable items are added, select the close button.
• To obtain a printout listing the last 10 dates an item was changed, select F3 Print.

Additional Instruction
• Please see additional instruction on maintenance procedures in the maintenance videos
available within online help.

Preparation of Solutions for Maintenance Procedures:

Bleach (Sodium hypochlorite) and Beckman Coulter Wash Solution are recommended for all
cleaning procedures related to the Beckman Coulter Chemistry-Immuno systems. Deviations
from related procedures or use of any other solutions should be evaluated by the institution to
ensure proper performance and compatibility with Beckman Coulter systems.

Bleach
For the purposes of maintenance procedures on Beckman Coulter Chemistry-Immuno systems,
undiluted bleach is defined as 5-10% Sodium hypochlorite, and may also contain up to 0.5-1%
Sodium hydroxide. Many brands of liquid household bleach contain impurities or lack sufficient
concentration of sodium hypochlorite for cleaning. Consequently, only the following should be
used. Do not use scented formulations of these products:
• Clorox®
• Javex-5™
• Reagent grade or other purified preparations of sodium hypochlorite
Prepare working concentrations by mixing bleach and DI water in the following proportions:
Concentration Volume of Clorox Volume of DI Water
10% 10 + 90
20% 20 + 80

NOTE: Sodium hypochlorite quickly loses its germicidal action upon exposure to light,
therefore, all dilutions should be made daily and in an area away from direct light. A 24-hour
preparation of this solution under an average fluorescent light environment is not sufficient to
cause degradation of the solution, provided the solution is placed into a closed container. Clear
containers are suitable for 24-hour storage of this solution.

Beckman Coulter Wash Solution

The catalog numbers for this product are OSR0001 and ODR2000.
Prepare working concentrations by mixing wash solution and DI water in the following
proportions:
Concentration Volume of Wash Solution Volume of DI Water
1% 1 + 99
2% 2 + 98
20% 20 + 80

F-4 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
May 19, 2006
 Month ____________ Year ________

F-5
DAILY ANALYZER 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Chapter F - Maintenance
2.1 Inspect Sample and
Reagent Syringes for
leaks
2.2 Check Wash Solution
Rolling Pump for leaks
2.3 Inspect concentrated
wash solution level
2.4 Inspect and clean
Sample & Reagent
probes and Mix Bars
2.5 Inspect the Stability of
the Upper Cover
2.6 Inspect printer and
paper
2.7 Prepare for a Sample
Probe wash
2.8 Change DI water in the
pre-dilution bottle
DAILY ISE 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
7.1.1 Inspect the ISE Reagent
Syringe for leaks
7.1.2 ISE Cleaning
WEEKLY ANALYZER 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Automated Chemistry Analyzer

3.1 Perform a W2

AU400/AU400e User's Guide

3.2 Perform a Photocal
3.3 Perform a Photometer
Check

April 14, 2010

3.4 Wash the Sample Pre-
Dilution Bottle
WEEKLY ISE 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
7.2.1 Perform a Selectivity
Check for the Na/K
Electrodes
Month _______________ Year ________

Automated Chemistry Analyzer

AU400/AU400e User's Guide
April 14, 2010
EVERY TWO WEEKS ISE 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
7.3 Clean the Mix Bars,
Liquid Level Lensors,
Sample Pot and
Sample Pot tubing.
MONTHLY ANALYZER 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
4.1 Clean Sample and
Reagent Probe Wash
Wells
4.2 Clean Mix Bar Wash
Wells
4.3 Clean Wash Nozzle, DI
Water Tank and Filter,
and Sample Probe
Filter.
EVERY THREE MONTHS
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
ANALYZER
5.1 Replace Wash Solution
Rolling Tube
5.2 Clean Air Filters
EVERY THREE MONTHS
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
ISE

Chapter F - Maintenance
7.4.1 Replace Mixture & Mid-
standard Pump Roller
Tubing (Part of 6 month
PM. User must perform
procedure alternately.)
7.4.2 Replace Valve Tubing
(Part of 6 month PM.
User must perform
procedure alternately.)

F-6
Month _______________ Year ________

F-7
AS NEEDED ANALYZER 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Chapter F - Maintenance
6.1 Replace Cuvettes
6.2 Replace the Sample
and Reagent Probes
6.3 Replace Mix Bars
6.4 Replace the Wash
Nozzle Joint Tubes
6.5 Replace Sample and
Reagent Syringes
6.6 Replace DI Water Filter
(Part of 6 month PM)
6.7 Replace Sample Probe
Filter (Part of 6 month
PM)
6.8 Replace Photometer
Lamp (Part of 6 month
PM)
6.9 Clean the Cuvettes and
the Cuvette Wheel (Part
of 6 month PM)
6.10 Clean or Replace
Individual Cuvettes
6.11 Perform a W1
Procedure
6.12 Clean Rack Feed Areas

Automated Chemistry Analyzer

6.13 Clean Wash nozzle unit

AU400/AU400e User's Guide

6.14 Clean Deionized-Water
Tank
6.15 Clean Deionized-Water
Filter

April 14, 2010

6.16 Clean Sample Probe
Filter
Month _______________ Year ________

Automated Chemistry Analyzer

AU400/AU400e User's Guide
April 14, 2010
AS NEEDED ISE 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
7.5.1 Replace the Reference
Electrode
7.5.2 Add Reference
Electrode Solution
7.5.3 Replace Reagents
7.5.4 Replace the Na, K, or
Cl Electrode
7.5.5 ISE Cleaning Procedure

F-8 Chapter F - Maintenance

2. Daily Maintenance
To obtain accurate results and optimum system performance, be sure to perform the following
daily maintenance procedures. Record maintenance on the schedules located at the beginning
of this chapter. Maintenance items can also be recorded on-line in the [Periodic Maintenance]
screen.

Caution
Do not place any body parts in the path of moving equipment, unless you are certain that the analyzer is
in Stop or Standby and that diagnostic functions are not activated.

Contents

2.1 Inspect Sample and Reagent Syringes for Leaks.................................................................. F-10

2.2 Check the Wash Solution Rolling Pump for Leaks................................................................. F-11
2.3 Inspect the Concentrated Wash Solution Level...................................................................... F-12
2.4 Inspect and Clean Sample Probe, Reagent Probe, and Mix Bars.......................................... F-13
2.5 Inspect the Printer and Paper................................................................................................. F-14
2.6 Prepare for a Sample Probe Wash......................................................................................... F-15
2.7 Change DI water in the Pre-dilution Bottle............................................................................. F-16

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-9
January 28, 2005
2.1 Inspect Sample and Reagent Syringes for Leaks
Before starting daily analysis, be sure to check the sample and reagent syringes for leaks or
condensation on the syringe case. If condensation is present, check for proper installation and
operation of the syringe. Check the sample and reagent syringes in the same procedure.
Possible
leakage
locations

If condensation or leaks are visible, perform the

following steps:

Caution
Do not place any body parts in the path of moving
equipment, unless you are certain that the analyzer is in
R S ISE R Stop or Standby and that diagnostic functions are not
activated.

Daily Procedure 3. Check the two fixing screws on the case head.
Prepare the following: If necessary, tighten them by turning them
OLP2061E
• Dry, clean cloth
clockwise.
4. Verify that the bottom screw fits securely against
1. Open the right front cover. the piston.
2. Check the following areas for leaks: bottom of 5. Visually check for leaks inside the syringe case.
the syringe cases, the case head, syringe case, Replace any damaged component.
area around the fixing screws and the tubing.
(Also make sure the tubing is not crimped.) To perform syringe verification for
troubleshooting, perform the following steps:
ISE reagent syringe
6. Loosen the bottom fixing screw first, then
Sample syringe
Mounting groove the top fixing nut. Pull the syringe and case
Reagent syringe
forward.
Fixing nut 7. Verify that the syringe provides a smooth,
Case head resistant pull by pulling on the piston.
FR 8. Turn the syringe case by hand. If the syringe
ON
T case is loose, turn it clockwise toward the case
Tubing head to tighten.
connectors
9. Visually check each case head for cracks. If
Syringe case
there are cracks on the case head, replace the
Piston fixing screw case head.
10. Pull the syringe from the case head and verify
See Also that there is one O-ring, and that it is not
For information about replacing and troubleshooting damaged.
syringes, refer to the“ As Needed Maintenance”
section of this Chapter and the Troubleshooting 11. Re-install the syringe and secure the top fixing
Chapter. nut first, and then the bottom fixing screw.
Note: Verify that the correct size syringe
(reagent or sample) is placed in the appropriate
Caution
position. To replace a syringe, refer to the “As
If your skin comes in contact with liquid, immediately Needed” section of this chapter.
rinse it with water.
12. Close the right front cover.

F-10 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
2.2 Check the Wash Solution Rolling Pump for Leaks
The wash solution rolling pump supplies concentrated wash solution to the wash solution tank.
If the pump leaks, the concentrated wash solution may not be diluted properly. Check the wash
solution rolling pump every day before starting analysis.

AU400e

Wash solution
rolling pump

Rolling tube

AU400

Connectors

Daily Procedure See Also

Prepare the following: For rolling tube replacement procedures, refer to the
• Dry, clean cloth section called "Maintenance Every Three Months."

1. Place the analyzer in standby or stop. 7. Check the tube connector. If it is loose, turn
clockwise and make sure it is finger tight.
2. Open the front left cover. Check for leaks again. If the tube still leaks,
replace it.
Caution
8. Close the left front cover.
If your fingers come into contact with any liquid,
immediately wash them with water. The wash solution
pump dispenses concentrated wash solution. Note
AU400 analyzers contain two Wash Solution Rolling
3. Blot the pump with the dry clean cloth. If the Pumps. AU400e analyzers contain one pump.
pump is wet, blot until completely dry.
4. Visually check the wash solution rolling tube for
cracks.
5. If cracks or other damage is found, replace the
wash solution rolling tube.
6. Verify that the labels on the tube match the
labels on the pump.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-11
January 28, 2005
 2.3 Inspect the Concentrated Wash Solution Level
Before starting daily analysis, check the wash solution level of the concentrated wash solution
tank. Add an appropriate amount of concentrated wash solution to refill the tank. If wash
solution becomes low or empty during analysis, an alarm is generated and the analyzer shifts to
OLP2083
a Pause Mode.

Master detergent
tank A
(factory optional) Master detergent
Master detergent tank A
NT tank B (factory optional)
FRO

Master detergent
tank B

NT
FRO
Daily Procedure
Prepare the following:
Warning
• •
• If the wash solution spills, wipe it up immediately
Wash Solution (#OSR0001) for tanks A (factory with a dry cloth. If the wash solution is not wiped
optional) & B up, a toxic gas may be generated and system
components could corrode.
1. Open the right front cover. • Be sure to use the correct wash solution
(OSR0001).

2. Visually check the wash solution level in tank

A (AU400 only) and B (AU400 and AU400e). 4. The capacity of each bottle is 2 liters. Either
The concentrated wash solution should be replace the bottle with a new one, or add
replenished daily. The following consumption concentrated wash solution to the existing bottle
chart is based on 2,000 tests per day: if it is not completely empty.

Concentrated wash solution: 5. Insert the liquid-level sensor into the

concentrated wash solution tank and tighten the
Approx. 0.40 L/day cap.
Adding Concentrated Wash Solutions 6. Push the tank back into position.
1. Pull the concentrated tank forward. 7. Close the right front cover.

Warning
When removing the cap from the concentrated wash
solution tank, do not allow your hands and clothing to
come into contact with the wash solution. If contact
occurs, immediately wash your hands with water for
at least fifteen minutes and refer to the MSDS sheet.
If wash solution comes into +contact with your eyes
or it is ingested, immediately rinse with water, avoid
vomiting and consult the MSDS sheet.

2. Loosen the wash solution tank cap, then pull out

the liquid-level sensor and the cap.
3. Take out the concentrated wash solution tank.

F-12 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 2.4 Inspect and Clean Sample Probe, Reagent Probe, and
Mix Bars
If problems are found with the sample probe, reagent probe, or mix bars, performance may be
affected. Before starting daily analysis, check that the sample probe, reagent probe, and mix
bars are operating properly. Also check the outside of each part for stains and crystallization.
Clean the parts with an alcohol wipe if necessary.
Daily Procedure Reagent probe arm Sample probe arm
■ Inspect for Normal Operation Mix bar unit
1. Open the upper cover.
2. Put the analyzer in Warm-up or standby.
3. Select [Maintenance], [ANL Maintenance].
4. Select “F/Prime Washing-line.”
5. Press the STAT ROTATION/DIAG button. The
DI water will be dispensed from each probe.
The mix bars are washed and rinsed, the wash
nozzle unit will do a sequence.
6. Observe the stream of DI water coming from the
sample and reagent probes. Verify that the wash
wells fill with water. If DI water sprays or does
not dispense in a straight line from the probe
tips, clean the probe tip with an Alcohol Prep Instruction
pad. A StyletOLP2012E
may be used if problems still occur Exercise care not to bend the sample probe, reagent
after wiping reagent probe tip. probe, or mix bars during cleaning.
Correct Incorrect

Stream
2. Visually check the sample probe, reagent probe,
and mix bars for bends or scratches.
If they are bent or scratched, replace them.

Caution
Replace the mix bar if there are chips in the teflon
coating. Chipped Mix Bars can cause carry-over and

inaccurate results.
7. Close the upper cover.
8. Optional Step: For inspection and cleaning
procedures, refer to the following. Chips

See Also
For replacement procedures, refer to the "As
Needed Maintenance" section of this chapter.
■ Inspecting and Cleaning the Sample Probes,
Reagent Probes, and Mix Bars

1. Inspect the probes and mix bars. If there is
staining or crystallization on the outside of the See Also
sample probe, reagent probe, or mix bars, clean For information about how to replace the sample or
the part with an Alcohol Prep. reagent probe or mix bars refer to the "As Needed
Maintenance” section of this chapter.
Automated Chemistry Analyzer
AU400/AU400e User's Guide Chapter F - Maintenance F-13
January 28, 2005
2.5 Inspect the Stability of the Upper Cover
Before starting daily analysis, check the stability of the upper cover of the analyzer to verify that
it is stable and remains in the upright position when raised. If the upper cover starts to descend
when opened, have the cover supports inspected and replaced by Beckman Coulter authorized
personnel.

Upper Cover

F-13a Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 14, 2010
 This page intentionally left blank.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-13b
April 14, 2010
2.6 Inspect the Printer and Paper
Before starting daily analysis, check the amount of paper in the printer. Check that the printer
is on and paper is loaded. If the printer paper is depleted during analysis, or if the power to the
printer is turned off, an error will result. During the error, data transferred to the printer will not
be printed.

See Also
For information about how to use the printer, refer to the operator's manual supplied with the
printer.

Daily Procedure
Prepare the following:
• Printer paper
1. Check that the printer is on. If not, turn on the power to the printer. Make sure that the
printer is online.
2. Check that the paper is loaded correctly and that a sufficient amount of paper remains. If the
paper is not loaded correctly, reload it.

See Also
For information about remedies for printer problems and how to load the paper, refer to the
operator's manual supplied with the printer.

F-14 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
2.7 Prepare for a Sample Probe Wash
Place a tube of 2% diluted wash solution (product number OSR0001) in the W1 (Wash 1)
position on the STAT table. When the analyzer shifts from Standby to Measure 1, the sample
probe will automatically go into the wash solution tube 5 times to be cleaned. If wash solution
is not on the STAT table in the W1 position, a "Sample Probe Detergent Short" alarm will be
generated. The probe is cleaned during the Measure 4 Mode after analysis is complete.

Note:
By following good lab practice and ensuring a clean, sealable container is used, diluted OSR0001 can be
prepared ahead of time. Diluted OSR0001 is good for 6 months from the date of preparation.

W1 Position

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-15
January 28, 2005
2.8 Change DI water in the Pre-dilution Bottle
Before starting daily analysis, rinse the sample pre-dilution bottle with DI water twice then fill it
with fresh DI water.

OLP2017f

Pre-dilution bottle

T
ON Pre-dilution position
FR

Caution 
Place the bottle in the compartment so it does not protrude above the surface of the analyzer top. If it is
not placed properly, reagent probe crashes could occur. Also, do not place the cap on the bottle when it
is on the analyzer, This also causes probe crashes.

F-16 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
3. Weekly Maintenance
To obtain accurate results and optimum system performance, be sure to perform the following
weekly maintenance procedures. Record maintenance on the schedules located at the beginning
of this chapter. Maintenance items can also be recorded on-line in the [Periodic Maintenance]
screen.

Contents

3.1 Perform a W2 (Washes Cuvettes, Mix Bars, Reagent Probes, and Waste Lines)................. F-18
3.2 Perform a Photocal (after the W2).......................................................................................... F-20
3.3 Perform a Photometer Check................................................................................................. F-21
3.4 Wash the Sample Pre-dilution Bottle...................................................................................... F-22

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-17
January 28, 2005
3.1 Perform a W2 (Washes Cuvettes, Mix Bars, OLP2122
Reagent
Probes, and Waste Lines)
If the sample probes, reagent probes, mix bars, and cuvettes are contaminated, appropriate analysis
results will not be obtained. The W2 prepares the cuvettes for the photocal by thoroughly cleaning them.
Perform a photocal to check the integrity of the cuvettes. The photocal can
STATbe performed
table cover after the W2
(small)
automatically by selecting this option when executing the W2.
STAT table

Note covers (large)

OLP2122
The W2 can be performed in conjunction with an End Process. Start the W2, wait until W2 Positioningdisplays
pin
in
the mode area. Verify that the system does not indicate “insufficient detergent? by allowing the timer to
reach 24 minutes. Exit System Status to Main Menu, then select the End Process STAT tablekey. The system can

be programmed to perform an automatic on with a Photocal, or a photocal can T be performed immediately

1 2
3
22
4
21
U-H U-L

5
20

S-
K-
L
ON

SE

6
after a W2.

L-N
a
19
FR

7
S-H
18

8
17

9
CLE W1

16
AN
W2 10
15
11
14
13 12

S
TA
T
E
N
S

D
TA
T

S
E
T
IS
E
P
R
IM
E
STAT table top view
STAT table cover
(small) 22
1 2
3
21

STAT table

4
U-H U-L

covers (large)

20

5
S-
L
K-S
EL
-N
a
19

6
S-H
18
Positioning pin

7
17

8
STAT table

16
CL
1 2
3

1
22

EA

9
W
4

N
21
U-H U-L
5

W2
T
20

S-L

W2
K-

ON
SE

15
L-
Na

10
19

FR
7
S-H
18

14
8

11

Position
17

13
9

CLE W1 12
16

AN
W2 10
15
11

Weekly Procedure
14
13 12
S
TA
T
E
N
S

D
TA
T

S
E
T
IS
E
P
R
IM
E

Prepare the following:

STAT table top view
a cleaning solution comes into contact with your
1 2
eyes or it is ingested, immediately rinse with water,
• Cleaning solution bottle (Empty 60ml reagent
22

avoid vomiting, and consult the MSDS sheet.

3
21

bottle.)
4

U-H U-L

• Do not spill the cleaning solution on the system. If

20

5
S-

cleaning solution spills onto the system, wipe it off

L

• Sample containers for W2 position on Stat Table

K-S
EL
-N
a
19

with a cloth or paper towel dampened with water,

6

(part #ZM0062).
S-H

then wipe 2 or 3 times using a dry cloth.

18

• 60 mL 1N HCl or • Do not mix the cleaning solution with other

17

60 mL of 10% bleach chemicals. If it has been mixed with another

16

chemical, discard it after performing an

CL

ISE Cleaning Solution: Optional for performing

EA 1
9

N W
W2

appropriate neutralization process.

15

10

Daily ISE Cleaning procedure during the W2 14

13 12
11

process.
• A W2 requires approximately 25 minutes to 1. Fill a sample tube (ZM0062) for the W2 with at
be performed. Alternate the cleaning solution least 3 mLs of HCl or bleach. If HCl was used
each week using a freshly prepared 10% bleach previously, then use bleach this time.
solution one week, and 1N HCl the next week. 2. Remove the two STAT table covers.

Warning 3. Set the sample container in the “W2” position

on the STAT table.
• Be careful to use the correct cleaning solution
before performing a W2. Do not mix an acid 4. Replace the two STAT table covers.
cleaning solution and an alkaline cleaning solution.
If they are mixed, a toxic gas may be generated. 5. Fill a 60 ml reagent bottle (OE63093) with 60
Prepare different bottles for each cleaning solution mLs of the specified HCl or bleach. If HCl
and note the cleaning solution name on each bottle was used the previous time, then use bleach this
to prevent them from accidentally being mixed. time. Do not fill the cleaning solution into the
• Do not allow your skin and clothing to come into neck of the reagent bottle or level sensing errors
contact with cleaning solution. If your hands could result.
or clothing come into contact with the cleaning
solution, immediately wash them off with water. If

F-18 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 OLP2017

Cleaning Solution
Bottle

T
ON Pre-dilution position
FR

the STAT table. "Start W2? Yes" will start the

W2 and ISE cleaning.
Caution 10. Select “YES,” then press enter. The system will
Be careful not to spill cleaning solution while handling start a W2. The operation will end after 25
the bottles. If cleaning solution spills onto the system, minutes. The time will count down in the mode
immediately wipe it off with a cloth or paper towel
display area.
dampened with water. Then wipe 2 or 3 times using a
dry cloth.
Important
6. Set the cleaning solution bottle in the pre- 11. After completing the W2 operation, replace
dilution position. the cleaning solution bottle with the pre-
dilution bottle. On the STAT table, remove
the sample tubes of cleaning solution from the
W2 and Clean positions.

12. Close the upper cover.

13. Perform a photocal measurement.

See Also
For information about how to perform a photocal,
refer to the "Weekly Maintenance” section in this
chapter.

7. Make sure the system power is on.
8. Select the [System Status] icon. The System
Status screen will appear.
9. Press function key F6 (W2 start). The W2 Start
window will appear. This window allows ISE
cleaning during the W2. Place a container of
ISE cleaning solution in the "Clean" position on

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-19
January 28, 2005
3.2 Perform a Photocal (after the W2)
The photocal procedure verifies the integrity of the cuvettes. Cuvettes that are dirty or
scratched will not pass the photocal procedure. Cuvettes that fail the photocal procedure could
affect analysis results, and they need to be cleaned or replaced. This procedure can also be
programmed to start automatically after an end Process and Auto-On. This is programmed in
[Parameters], [System], [Auto Power On]. The settings in system maintenance must be on for
this automatic maintenance setting to work. Additionally, a photocal can be performed with a
W2 (see 'Perform a W2' in this chapter).
Weekly Procedure Important
The Absolute check prompt must be set to 6.5 to max
Caution out the absolute check range. The absolute check will
To obtain accurate analysis data, do not start a photocal compare the OD of a cuvette to the last saved OD of
until the photometer lamp has stabilized (approximately the same cuvette. If the absolute check prompt is left
at 0.0 when function key F5 (check start) is selected,
20 minutes). all cuvettes will fail the absolute check, and appear in
blue on screen. The analyzer can be operated within
1. Make sure the system is in the Standby Mode. specification if the mean check and cuvette check are
within the acceptable range. The absolute check is not
2. Select the [System Status] icon. The [System a scheduled maintenance procedure.
Status] screen will appear.
2. Press function key F5 (check start). Any cuvette
3. Press function key F7 (Photocal Start). The
that fails the mean cuvette check will be listed in
Photocal Start window will appear.
the "Error Cuvette No. List" window in red. A
4. Select “YES,” then press enter. The system cuvette failing the internal cuvette check is listed
will start a photocal (takes 20 minutes). When in green.
the photocal is complete, the system moves to
3. Optional: Print a list of defective cuvettes by
Standby.
selection function key F3 (print). Note: The
internal cuvette check range is 0.01 and cannot
Checking Results
be changed.
1. Select [System Status], [Cuvette Status]. Verify
that a range of 0.03 for the mean check and a 4. Remove and clean all defective cuvettes on the
range of 6.5 for the absolute check is set as a list. For information on cleaning individual
default. If not, enter the ranges of 0.03 and 6.5 cuvettes, refer to the "As Needed" maintenance
in the appropriate fields. When function key section in this chapter.
F5 (check start) is selected in this screen, the 5. Repeat the photocal procedure after cleaning or
absolute check, cuvette check and mean check replacing any cuvettes. If an error occurs after
are performed. Only the mean check data is cleaning the cuvettes, replace them.
analyzed for routine operation. The absolute
check (or base check) cuvette data is not utilized 6. Select function key F6 (save) to save photocal
for routine operation. data after a successful photocal.

F-20 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 3.3 Perform a Photometer Check
The photometer check is performed to check the integrity of the lamp. A cuvette will be
checked for the 0% and 100% transmittance of light through the cuvette. The lamp is also
checked when the analyzer goes from Standby to a Measure Mode.
A new lamp, at 100% values, will be closer to 0.01 then move toward 1.7 as the lamp ages. It is
normal to see the 340 wavelength at a slightly higher reading than the other 12 wavelengths.

Weekly Procedure
Prepare the following: 5. Select a cuvette number (1-88) to perform the
• DI water check.
6. Add approximately 500 ul of DI water to the
Caution cuvette number selected with a pipet.
To obtain accurate results, do not start a photometer 7. Select "Yes" at the Start Measure prompt.
check until the photometer lamp has stabilized after
system start-up (approximately 20 minutes). 8. The 0% and 100% values will display on the
screen.
Acceptable Ranges: 0% = -5 to 25
Instruction
The analyzer must be in the Stop mode to enter
100% = 0.01 to 1.7
diagnostics. If the numbers exceed these ranges, replace
the photometer lamp and perform a photocal.
1. Transfer the unit to the Stop Mode, then select After the lamp is replaced, the operator must
[Maintenance], [Maker Maintenance], [ANL repeat a photocal with the new lamp. Refer to
DIAG]. the replace Lamp procedure in the As Needed
2. Select the "Combination" tab located at the top Maintenance section.
of the screen.
Instruction
3. Select "Photometer Check A/0-100%."
The water will automatically be removed from the
4. Select the "Print" check box to print the results. cuvettes during the next wash or start process.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-21
January 28, 2005
3.4 Wash the Sample Pre-dilution Bottle
The pre-dilution bottle must be cleaned weekly with bleach to prevent bacteria from growing
inside which could affect analysis results.
OLP2017f

Pre-dilution bottle

T
ON Pre-dilution position
FR

Weekly Procedure Caution

Prepare the following: Place the bottle in the compartment so it does not
protrude above the surface of the analyzer top. If it is
• DI water not placed properly, reagent probe crashes could occur.
• Freshly prepared 10% bleach solution Also, do not place the cap on the bottle when it is on
the analyzer, this also causes probe crashes.
1. Remove the pre-dilution bottle from the analyzer
and empty it.
2. Rinse the bottle with a freshly prepared 10%
bleach solution and empty it.

Caution
Do not leave any bleach residue in the bottle! Residue
will adversely affect analysis results.

3. Rinse and empty the bottle with DI water twice.

4. Fill the bottle with fresh DI water and place
the bottle in the pre-dilution position on the
analyzer.

F-22 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
4. Monthly Maintenance
To obtain accurate results and optimum system performance, be sure to perform the following monthly
maintenance procedures. Record maintenance on the schedules located at the beginning of this chapter.
Maintenance items can also be recorded on-line in the [Periodic Maintenance] screen.

Contents

4.1 Clean the Sample Probe and Reagent Probe Wash Wells.................................................... F-24
4.2 Clean the Mix Bar Wash Wells............................................................................................... F-25
4.3 Clean the Wash Nozzle Unit, Deionized-Water Tank and Filter, and Sample Probe Filter..... F-26

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-23
January 20, 2006
 4.1 Clean the Sample Probe and Reagent Probe Wash Wells
If the sample or reagent probe wash wells are not cleaned regularly, they will become increasingly difficult
to clean. Stains may cause contamination and reduce the reliability of analysis data. A stained sample
probe may also contaminate samples. To keep analysis data reliable, and to prevent samples from being
contaminated, clean the sample and reagent probe wash wells every month.

OLP2018E

Reagent probe

Reagent probe
wash well
Sample probe

T
ON
FR Sample probe wash well

Monthly Procedure
Caution
Prepare the following:
If bleach spills, immediately wash the affected area
• Squirt bottle with freshly prepared 10% bleach with water.
solution
• Cotton swab 6. Squirt the 10% bleach solution into each wash
well while cleaning the inside with a cotton
1. Open the upper cover. swab. During cleaning, be careful not to
2. Verify that the analyzer is in the Warm-up or damage the sample and reagent probe tips.
Standby Mode. 7. Press the stat rotation/diag button. The sample
3. Select [Maintenance], [ANL Maintenance]. and reagent probe will return to its home
position over the wash well.
4. Select “D/Cleaning Wash Wells.”
8. Select “F/Prime Washing-line” on the
5. Press the STAT ROTATION/DIAG button. The [Maintenance], [ANL Maintenance] screen.
sample and reagent probes move to the cuvette
wheel position. 9. Press the STAT ROTATION/DIAG button. The
DI water is dispensed from the sample and
reagent probes into the wash wells.
9. Close the upper cover.

F-24 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
4.2 Clean the Mix Bar Wash Wells
If the mix bar wash wells are not cleaned regularly, deposits mayOLP2020E
form in them. This causes
contamination and reduces the reliability of analysis data. The stained mix bars may contaminate
samples. To maintain the reliability of analysis data, and to prevent samples from being
contaminated, clean the mix bar wash wells every month.

Mixing bar

Mixing unit

T
ON
FR

Monthly Procedure
Prepare the following:
• Squirt bottle with freshly prepared 10% bleach
solution
• Cotton swab
1. Open the upper cover.
2. Verify that the analyzer is in the Warm-up or
Standby Mode. Caution
3. Manually turn the mix bar units so the mix bars Do not spill the bleach solution outside the mix bar
are not over the wash wells. wash well. If it spills, immediately wipe it up.
4. Squirt the 10% bleach solution into each wash
well while cleaning the inside with a cotton 6. Select “F/Prime Washing-line” on the
swab. [Maintenance], [ANL Maintenance] screen.

5. Realign the mix bar unit as closely as possible 7. Press the STAT ROTATION/DIAG button. The
to avoid errors. mix bars will go through one wash sequence.
8. Close the upper cover.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-25
January 28, 2005
4.3 Clean the Wash Nozzle Unit, Deionized-Water Tank and
Filter, and Sample Probe Filter..
The following four procedures share several steps, so it is most efficient to perform them
together. If only one procedure is required while troubleshooting a particular problem, locate the
procedure in the As Needed section of this chapter.

Wash Nozzle Unit

The wash nozzle unit is equipped with a total of eight nozzles. The unit consists of six wash
nozzles (three-part nozzle) for mixture aspiration, wash solution/deionized-water (DI-water)
dispensing, and wash solution/DI water overflow aspiration, and one nozzle each for aspiration
and drying. If a nozzle is clogged, or the O-ring on the tube mounting joint manifolds are
damaged, flattened, twisted or missing, the function of the nozzle does not work properly. This
results in a cuvette wheel overflow or problems with the analysis data. To prevent an overflow
and abnormal analysis data, clean the wash nozzle unit every month.

Deionized-Water Tank
Regular cleaning prevents the accumulation of deposits or bacteria that could affect analysis.

Deionized-Water Filter
If the deionized-water filter is dirty, the system may generate abnormal analysis data.

Sample Probe Filter

If the sample probe filter is dirty, the system may generate abnormal analysis data.

Monthly Procedure
Prepare the following:
• Dry, clean cloth • Freshly prepared 20% bleach
• Sonicator filled with DI water • Extra DI tank (In start-up kit)
• Basin

F-26 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 20, 2006
 Tube
Tube mounting
joint

Tube mounting
joint manifold

Knob

Tube mounting Positioning screw

joint manifolds

Wash nozzle
Wash nozzle FR
ON
station T

8. Loosen the knob on the wash nozzle unit, then

remove the wash nozzle unit along with the
tubing.

Caution
After placing the wash nozzle unit in the sonicator with
DI water, verify that the tips of the wash nozzle do not
rest on the bottom of the sonicator. When the sonicator
is running the wash nozzle to vibrate and can damage
the tips.

Instruction
• When cleaning the wash nozzle unit using a
sonicator, be careful not to scratch the wash
Remove and Clean the Wash Nozzle Unit nozzles.
1. Open the upper cover. • Do not scratch or tear the joints and tubes. A leak
may result from the scratched part and the cuvette
2. Verify that the analyzer is in the Warm-up or wheel may overflow.
Standby Mode.
3. Select [Maintenance], [ANL Maintenance]. Tips
4. Select “E/Replacing Wash Nozzle.” The sonicator is the optimal device for cleaning the
wash nozzles. If a sonicator is not available, use DI
5. Press the STAT ROTATION/DIAG button. The water. While pouring water into the wash nozzles, clean
liquid in the tubing on the wash nozzle unit is each nozzle hole using the supplied stylet. Rinse the
drained. nozzles in DI water, then dry them with a soft cloth.
6. Open the rear cover.
9. Clean the tips of the nozzles for 15 minutes
7. Remove the four tube mounting joint manifolds using DI water in a sonicator. No wash solution
from the wash nozzle unit. is required.
Do not submerge the springs on top of these
Instruction nozzles - only submerge the tips of the wash
• When handling the wash nozzles, be careful not to nozzles, including the shortest nozzles tips.
scratch them.
• When loosening the knob on the wash nozzle unit,
do not loosen the positioning screws on both sides
of the knob. These screws are used for positioning
the wash nozzle unit.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-27
January 20, 2006
 OLP2024E

Joint
Connecting hose
Button Fixture
Top

Filter case

Button
NT Joint
FRO Basin

Remove and clean the Sample Probe Filter

Instruction TOP
Perform an End Process before removing the Sample
Probe Filter, DI-Water Filter, or DI-Water Tank. If this
Button
procedure is performed with the system sub-power on,
water could spill from tubing and tanks.

1. Confirm that an End Process was performed.

2. Open the left front cover.
Cap
3. Position a basin to catch any liquid that spills
from the deionized-water drain hose.
4. Pull the Sample Probe Filter Case forward to
remove it from the metal clip. Filter Positioning
Ring
5. While pressing the button at each end of the
sample probe filter case, disconnect the hose
joints. Sample Probe
Filter
Instruction
Loosen the filter case over the basin. The deionized
water in the filter case will drain from the joint. If water O-ring
spills onto the system, immediately wipe it up with a
dry, clean cloth.
When removing the sample probe filter, be careful not
to lose the O-ring or the filter positioning ring.

Case
6. Unscrew the Sample Probe Filter Case and
remove the sample probe filter.
7. Clean the Sample Probe Filter for 10 minutes,
using DI water in a sonicator. If a sonicator
is not available, rinse in DI water. While
pouring DI water onto the filter, scrub it with a
Button
toothbrush.
8. Inspect the O-ring, Filter Positioning Ring,
and the inside of the case. Remove any
accumulations with a damp towel.

F-28 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
Remove the Deionized-Water Tank
ÊÊOLP2033E
Cap
Tip
If low-quality deionized water is obtained from the
main water source, the two Diluted Wash Solution Float switch
Tanks may require cleaning in addition to the
Deionized-Water Tank. For detailed information,
T
contact Beckman Coulter Technical Services. FR
ON

Deionized-water tank Supply

Tubing

Connector
Deionized-water tank
Float switch

Fixture

Joint

Tubes
Filter case Deionized-water
drain hose

T
ON
FR

Basin

Remove the Deionized-Water Filter

Instruction
Loosen the DI-Water Filter case over the basin. The
OLP2023E
deionized water in the filter case will drain from the
joint. If the deionized water spills onto the system,
immediately wipe it off with a dry, clean cloth.

1. Turn the DI-Water Filter case cap to remove it.

2. Remove the deionized-water filter from the filter
case.

1. Unplug connector #240.
2. Disconnect the joint of the deionized water
drain hose from the tank by pressing the gray
Quick Disconnect button.
3. Pull the DI water tank forward while removing
the tubes inserted in the tank. Lay the tubes in
Water supply tube
the basin.
4. Remove the deionized-water tank from the Filter case

instrument. Basin

5. Loosen the water tank cap and remove the float

switch. Deionized water filter
ONT
FR Filter case cap

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-29
January 28, 2005
Clean both filters and the Deionized-water Re-install the Sample Probe Filter
Tank 1. Rinse the Sample Probe Filter in deionized water.
1. Clean the deionized-water filter for 10 2. Insert the Sample Probe Filter into the filter case.
minutes in DI-water using a sonicator. If a
sonicator is not available, pour DI water over 3. Position the O-ring correctly on the filter case.
the filter while scrubbing it with a toothbrush. 4. Place the Filter Positioning Ring over the filter.
2. Rinse the tubes and inside of the tank 5. Assemble the top of the filter case (cap) and
with a fresh 20% bleach solution and rinse hand‑tighten. Ensure the button at the top of the
thoroughly with deionized water. filter case (cap) faces the same direction as the
3. Store the tank where it can air dry. bottom button of the filter case.
6. Refer to the illustrations and verify that the top of
Re-install the Deionized-water filter the filter case is pointed upward and connect the
two hoses to the filter case fittings. Push each
1. Rinse the DI-water Filter in deionized water.
hose connector into the filter case until a click is
2. Insert the DI-water Filter into the filter case. heard.
3. Replace the Filter Case cap and hand-tighten. TOP

Install the spare Deionized-water Tank
Button
1. Fill the spare DI tank at least half full with DI
water and install the tank. Alternate the tanks so
each one can dry thoroughly between cleaning.

Caution
If the tank is empty, and the pump runs without water, Cap
the pump may be damaged.

2. Insert the the Float Switch and water supply

tubes into the Deionized-water Tank. Tighten the Filter Positioning
Ring
cap.
3. Replace the DI-water Tank in the system. Verify
that the tubing is fully inserted in the DI-water Sample Probe
Tank. Filter

4. Reconnect the joints on the deionized-water

tank. Push each joint until a click is heard.
O-ring
5. Reconnect connector #240. When reconnecting
the #240 connector, do not force the connection
or the pins can be damaged. Turn the connector
gently until it slides into the connection easily
and without force.
Case

Button

F-30 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 20, 2006
Re-install the Wash Nozzle Unit 6. Close the rear cover.
1. Remove the wash nozzle unit from the sonicator
and wipe with a soft cloth. Check the system
1. Press the Green power button to apply power to
2. Using a gauze dampened with water, wipe any
to the system.
foreign matter from O-rings such as dust or
detergent residue. If foreign matter remains, 2. After initialization, select [Maintenance], [ANL
remove the O-ring with tweezers, wash with Maintenance].
water, and re-insert.
3. Select "F/Prime Washing-line."
3. Place the O-ring inside the depense manifold.
4. Press the STAT ROTATION/DIAG button.
If any O-ring is scratched or damaged, replace
with O-ring MU9638. • Observe the DI-Water and filter tubing. The
deionized water will flow through the tube
Caution and then the air in the DI-Wiater tubing
will be released. This prime may need to be
If O-rings are used without cleaning for an extended
period of time, or if the cover on the jount unit is performed 2-3 times to ensure that all of the
closed without any O-ring set in the groove, detergent air is out of the tubing.
crystals will collect and could scratch the O-rings.
Check the O-rings during Monthly mainteance. For • Observe the Wash Nozzle Unit. Verify that
more reliable maintenance, replace the O-rings once a air in the tubing of the wash nozzle unit is
year. released. Verify the movement is smooth and
complete.

Instruction
Be sure to attach the tube mounting joint manifolds 5. Repeat the previous step two or three times
in their original places. If a manifold is attached until the air in the affected tubing is completely
incorrectly, normal analysis cannot be performed. removed.
6. Close the left front cover and the upper cover.
4. Match the two positioning holes on the wash
nozzle unit with the positioning screws, then
secure the station by finger-tightening the knob.
5. Attach the manifolds, hand tighten them firmly.
When attaching the manifolds, match the color
connections and tighten them firmly. For
information about the tube mounting joint
positions, refer to the drawing on the following
page. Make sure the O-rings are in place on
each manifold and not damaged.
OLP2120.eps

Type 1 Type 2

O ring MU9638 O ring MU9638

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-31
January 20, 2006
Tubing Diagram of the Wash Nozzles and Tube Mounting Joints
In order to distinguish individual connecting positions easily, follow the tubing diagram below:

A - Aspirate
B - Overflow
C - Dispense

F-32 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 20, 2006
5. Maintenance Required Every Three
Months
To obtain accurate results and optimum system performance, perform the following maintenance
procedures every three months. Record maintenance on the schedules located at the beginning
of this chapter. Maintenance items can also be recorded on-line in the [Periodic Maintenance]
screen.

Contents

5.1 Replace the Wash Solution Rolling Tube............................................................................... F-34

5.2 Clean Air Filters...................................................................................................................... F-35

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-33
January 20, 2006
 OLP2062E
5.1 Replace the Wash Solution Rolling Tube
The rolling tube will wear and break if it is used for an extended period of time.

Rolling tubes

Wash solution
rolling pump A
Included on AU400
only

OLP2063 Wash solution

rolling pump B
T
ON
FR

Rolling tube connectors

Groove

Relay tubes
T
ON
FR

Matching
Every Three Months numbers
Prepare the following:
• New tubing MU9623
1. Place the analyzer in Warm-up or Standby.
2. Open the left front cover.
3. Select [Maintenance], [ANL Maintenance].
4. Select "G/Replace Detergent Tube." 7. Connect the new tubing and rotate the
5. Press the STAT ROTATION/DIAG button. The connectors to tighten them.
pump rotates in reverse. The concentrated wash 8. Stretch the tubing around the rolling pump.
solution in the tube returns to the tank. Slip the tube into the groove as shown in the
illustration. Note: The numbers on the tube
Note should match the numbers on the side of the
The AU400 includes two pumps. The AU400e only pumps.
contains one pump.
9. Press the STAT ROTATION/DIAG button.
The pump rotates counterclockwise. The
Caution concentrated wash solution fills the tubing.
Handle the tubing with care or wash solution could 10. Check for leaks in the tubing then close the left
splash on skin. If wash solution splashes on your skin, front cover.
immediately wash the area with water.

6. Remove rolling tube A (factory optional) and

B shown in the illustration above. Remove the
tubes by unscrewing the connectors of each
tube.

F-34 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
5.2 Clean Air Filters
If the air filters on the system are used for an extended period of time, they may become
clogged with dust and dirt. This reduces the cooling performance in the inside of the
system. Clean the air filters every three months. OLP2027E

FR
ON
T

Air filter(S)
MU9593

Air filter(L)
MU9594

Air filter(S)
MU9593

FR
ON
T

Every Three Months

Prepare the following:
• Vacuum

Caution
Do not clean the air filters with water. Moisture could 3. Vacuum the air filters thoroughly.
enter the system and damage the internal electronics. 4. Place the air filters back in their original
locations.
1. Confirm that the system is is in Standby mode.
2. Remove the three air filters as shown in the
figure.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-35
January 28, 2005
F-36 Chapter F - Maintenance
Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
6. As Needed Maintenance
To obtain accurate results and optimum system performance, perform the following maintenance
procedures as needed. Record maintenance on the schedules located at the beginning of this
chapter. Maintenance items can also be recorded on-line in the [Periodic Maintenance] screen.

Contents

6.1 Replace the Sample and Reagent Probes............................................................................. F-38

6.2 Replace Mix Bars.................................................................................................................... F-40
6.3 Replace the Wash Nozzle Joint Tubes................................................................................... F-41
6.4 Replace Sample and Reagent Syringes................................................................................. F-44
6.5 Replace the Photometer Lamp (Part of 6 month PM)............................................................ F-46
6.6 Clean or Replace Individual Cuvettes.................................................................................... F-48
6.7 Clean the Cuvettes and the Cuvette Wheel (Part of 6 month PM)......................................... F-49
6.7a Clean the Cuvettes and Cuvette Wheel after a Cuvette Wheel Flood.................................. F-50a
6.8 Perform a W1 Procedure........................................................................................................ F-51
6.9 Clean Belts and Rack Feed areas.......................................................................................... F-52
6.10 Clean the Wash Nozzle Unit................................................................................................... F-53
6.11 Clean or Replace the Sample Probe Filter............................................................................. F-56
6.12 Clean the Deionized-Water Tank............................................................................................ F-58
6.13 Clean or Replace the Deionized-Water Filter......................................................................... F-60
6.14 Clean or Replace the Static Discharge Brushes.................................................................... F-62

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-37
August 29, 2008
 OLP2052E

6.1 Replace the Sample and Reagent Probes

If the sample or reagent probes are damaged or have occlusions that cannot be removed, replace
them.

Sample probe cover

OLP2053E

T
ON
FR

As Needed
Probe connector

Sample Probe Replacement Procedure Sample probe

Prepare the following:

• Sample probe MU9934

Instruction ON
T
FR Sample probe wash well
Check that the sample probe is positioned over the
wash well before replacing it. Liquid will drip during
probe replacement.
10. Tighten the probe connector to secure the
sample probe. Tighten the connector firmly.
1. Verify that the analyzer is turned on and is in the
Warm-up or Standby Mode. 11. Replace the sample probe cover. First place it
on the arm from the probe side. A click should
2. Open the upper cover.
be heard.
3. Select [Maintenance], [ANL Maintenance].
12. From [Maintenance], [ANL Maintenance] select
4. Select "A/Replace S Probe & Syringe." "F/Prime Washing-line."
5. Press the STAT ROTATION/DIAG button. 13. Press the STAT ROTATION/DIAG button. The
Water will drain from the sample probe tubing. DI water will be dispensed from the probe tip.
Check that the DI water is dispensed in a thin
6. Remove the sample probe cover. The probe
straight stream. It should not spray or dispense
cover has wedges inside the cover. While
at an angle.
spreading both sides of the cover from the inside
by hand, lift the cover to remove it from the 14. Close the upper cover.
probe arm.
7. Disconnect the probe connector.

Instruction
When replacing the sample probe, be careful not to
bend or scratch the probe tip.

8. While holding the probe connector, pull the

sample probe upward.
9. Place a new sample probe through the top of the
holder.

F-38 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 OLP2054E OLP2055E

Probe connector

Reagent probe

Reagent probe cover

Reagent probe wash well Reagent probe wash well

T T
ON ON
FR FR

As Needed Instruction
Reagent Probe Replacement Procedure When replacing the reagent probe, be careful not to
bend or scratch the probe tip.
Prepare the following:
• Reagent probe MU9958
8. While holding the probe connector, pull the
reagent probe upward.
Instruction
9. Place a new reagent probe through the top of the
Check that the reagent probe is positioned over the
wash well before replacing it. Liquid will drip during holder.
probe displacement. 10. Tighten the probe connector to secure the probe.
Tighten the connector firmly.
1. Verify that the analyzer is turned on and is in the 11. Replace the probe cover. First place it on the
Warm-up or Standby Mode. arm from the probe side. A click should be
heard.
2. Open the upper cover.
12. From [Maintenance], [ANL Maintenance], select
3. Select [Maintenance], [ANL Maintenance].
"F/Prime Washing-line."
4. Select "B/Replace R Probe & Syringe."
13. Press the STAT ROTATION/DIAG button. The
5. Press the STAT ROTATION/DIAG button. DI water will be dispensed from the probe tip.
Water will drain from the reagent probe tubing. Check that the DI water is dispensed in a thin
straight stream. It should not spray or dispense
6. Remove the reagent probe cover. The probe
at an angle.
cover has wedges inside the cover. While
spreading both sides of the cover from the inside 14. Close the upper cover.
by hand, lift the cover to remove it from the
probe arm.
7. Disconnect the probe connector.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-39
January 28, 2005
6.2 Replace Mix Bars
OLP2056E
Replace the mix bars if they are stained, damaged, or if the teflon coating is chipped.

Mix bar

Mix unit

T
ON
FR

As Needed
Prepare the following:
Chips
• New Mix Bar MU9599

Caution
Replace the mix bars while the mix unit drive is not
operating. Personal injury may result if replacement of
a mix bar is attempted during operation of the mix unit.

1. Verify that the analyzer is turned on and is in the

Warm-up or Standby Mode.
2. Open the upper cover.
3. Pull out the mix bar to be replaced.
4. Insert a new mix bar into the mix unit from the
5. Select [Maintenance], [ANL Maintenance].
top. Be careful not to sctatch the mix bar when
inserting it into the mix unit. While inserting 6. Select "F/Prime Washing-line."
the mix bar, rotate it slightly to engage the flat
7. Press the STAT ROTATION/DIAG button.
portion of the bar with the gear of the mix unit.
Observe the mix bars during the wash sequence.
8. Close the upper cover.

F-40 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 6.3 Replace the Wash Nozzle Joint Tubes
If a wash nozzle tube is scratched or cracked or liquid drips from nozzles, liquid could remain in
the cuvettes, or the dripping could cause an overflow. This may result in an analysis data error.
OLP2085E
To prevent analysis data errors, immediately replace the damaged tube.

Tube
Tube mounting
joint

Tube mounting
joint manifold

Knob

Tube mounting Positioning screw

joint manifolds
OLP2058E
Wash nozzle
Wash nozzle FR
ON
station T

Joint tube

Tube
Instruction
• When handling wash nozzles, be careful not to
scratch them.
Joint tube
• When loosening the knob on the wash nozzle unit,
do not loosen the positioning screws on both sides
of the knob. These screws are used to position the
wash nozzle unit.
Wash nozzle
station
8. Loosen the knob on the wash nozzle unit, then
remove the wash nozzle unit along with the
tubing. Put it on a flat working surface.
As Needed
9. Remove the tubes to be replaced.
Prepare the following:
• New joint tube ZM1131 (3 pieces per set) Caution
1. Open the upper cover. Replace tubes one by one. If a wrong nozzle and tube
are connected, problems could occur. Refer to the
2. Verify that the analyzer is turned on and is in the drawing on the next page for correct tube and nozzle
Warm-up or Standby Mode. positions.

3. Select [Maintenance], [ANL Maintenance].

10. Place the new joint tube on the mounting joint
4. Select “E/Replacing Wash Nozzle.” and on the other end of the wash nozzle unit.
5. Press the STAT ROTATION/DIAG button. The Both ends of the tubing should be centered.
liquid is drained from the tubing. Allow approximately 1mm between the ends of
the tube and nozzle as shown in the drawing.
6. Open the rear cover.
7. Remove the four tube mounting joint manifolds
from the wash nozzle unit.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-41
January 28, 2005
Instruction
Be sure to attach the tube mounting joint manifolds Check the system
in their original places. If a manifold is attached 1. Select [Maintenance], [ANL Maintenance].
incorrectly, normal analysis cannot be performed.
Select "F/Prime Washing-line."
14. Replace the wash nozzle unit and reconnect 2. Press the STAT ROTATION/DIAG button. The
the four tube mounting joint manifolds. When deionized water flows through the tube and the
attaching the manifolds, match the color air in the tube is removed.
connections and tighten them firmly. 3. Close the upper cover.
15. Close the rear cover.

F-42 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 OLP2112E OLP2058E

Joint tube

Tube

Cross-sectional View
Joint tube
Tube
Joint tube
Position both ends
of the tube and Approx.
nozzle in the center 1mm
Wash nozzle
Wash nozzle of the joint tube.
station
station
Nozzle

A - Aspirate
B - Overflow
C - Dispense

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-43
January 28, 2005
 OLP2059E
6.4 Replace Sample and Reagent Syringes
Replace the syringe when: 1. Pulling on the piston, there is no longer a smooth, resistant pull.
Instead, the syringe is very hard or loose when pulling, or it is not a smooth movement.
2. The teflon tip is worn or damaged. 3. There are leaks around the syringe even after proper
installation. 4. The head of the syringe is cracked.

ISE Reagent syringe

Fixing nut
Sample syringe
Reagent syringe
Mounting groove

Tubing
Connectors

FR
ON
Syringe case T

Solenoid Mounting groove

valve
Piston fixing screw

Fixing
nut 3. Select [Maintenance], [ANL Maintenance].
4. Select one of the following menu items
Case
head according to the type of syringe to be replaced.
To replace the sample syringe, select “A/Replace
S Probe & Syringe.” To replace the reagent
Syringe syringe, select “B/Replace R Probe & Syringe.”
case
5. Press the STAT ROTATION/DIAG button.
Liquid is drained from the tube of the selected
syringe.
Syringe
Caution
Piston Do not place any body parts in the path of moving
fixing equipment, unless you are certain that the analyzer is in
screw
Stop or Standby and that diagnostic functions are not
activated.
Reagent Sample ISE Reagent
6. Loosen the bottom fixing screw first, then
As Needed the top fixing nut. Pull the syringe and case
Use the following procedure to replace the sample forward.
syringe and reagent syringes.
Caution
Prepare the following: • Do not apply excessive force to the fixing screws
when removing the syringe case. This can cause
• New sample syringe ZM0111 damage to the syringe case. Do not use pliers
when tightening the screws.
• New reagent syringe ZM0112
• If the relay tubes are disconnected from the
• (Same for ISE Reagent [Buffer] syringe.) solenoid valve during syringe case removal, screw
the relay tubes onto the connectors.
Remove and Replace Syringes
7. Tilt the syringe head and case upside down
1. Open the right front cover.
before replacing the syringe. This will prevent
2. Verify that the analyzer is turned on and is in air from entering the tubing lines and keeps the
the Warm-up or Standby Mode. water that collects in the head of the syringe
from leaking.

F-44 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 OLG2060E

Case head

O-ring

Syringe
OLP2061E
FR
ON
T

Syringe case

ISE reagent syringe

Sample syringe
Reagent syringe Mounting groove

Fixing nut

Case head
FR
ON
T
Tubing
connectors
Syringe case
8. To remove the syringe case, turn it Piston fixing screw
counterclockwise while holding the case head. Note: Top screws must be tightened first to ensure
9. Pull the syringe out from the case head. If proper syringe alignment on the analyzer.
the O-ring remains in the case head, carefully 13. Tighten the piston fixing screw (bottom screw)
remove it with tweezers to prevent scratching finger tight only. Do not use pliers.
the case head.
Check the system
Warning 1. Perform the following as required:
Verify that the correct size syringe is being replaced
(sample or reagent). The S and R syringe have • • If a sample syringe is replaced, select “H/S
different piston shaft diameters. Syringe Prime" on the [ANL Maintenance]
Piston shaft diameter: D screen. This takes approximately 25 minutes
to complete. Selecting F2 Exit stops the
D=5mm: R Syringe
D=2mm: S Syringe prime and exits the menu.
• • If a reagent syringe is replaced, select
Do not pull the piston out of a new syringe. This could
cause the syringe to be inaccurate even if it is replaced. “F/Prime Washing-line" on the [ANL
Maintenance] screen. Repeat this step
five or six times until the air in the tube is
10. Insert the new syringe into the case head completely removed. If the ISE Reagent
without dumping the existing water from the (Buffer) Syringe was replaced, select [System
head of the syringe. Water may escape on the Status], [ISE Status], F6 Prime, D/Buffer
side of the syringe head. Dry the excess water. Prime.
Completely dry the syringe and syringe case to
2. Press the Stat Rotation/Diag button. For Sample
prevent condensation from forming on the case.
or Reagent Syringes, DI water flows through the
11. Tilt the syringe head upward without twisting probe, removing air from the probe. On the ISE
the tubing lines. Screw the syringe case Buffer syringe, buffer flows through the tube and
clockwise onto the case head. “J” nozzle, removing the air.
12. Re-install the syringe and secure the top fixing 3. Close the right front cover.
nut first, and then the bottom fixing screw.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-45
February 1, 2005
6.5 Replace the Photometer Lamp
OLP2036E

Photometer lamps are under warranty for 1,000 hours. If the photometer lamp deteriorates,
appropriate analysis results will not be obtained. Replace the photometer lamp every six months,
or when the photometer check is out-of-range, or when alarms occur. After replacing the
photometer lamp, perform a photocal to obtain accurate data.

Lamp unit cover

Knobs
Lamp holder

T
ON
FR Lamp cords

Warning
• To prevent electric hazards, be sure to perform an
End Process before replacing the photometer lamp.
• Wait 5 minutes or more after the End Process
is completed. Do not touch the lamp with bare
hands until the photometer lamp has cooled down
completely. The lamp is very hot and can cause
burns.
• Never touch the glass of the photometer lamp with
bare hands or gloves! Oil from skin or fingerprints
changes the light intensity of the lamp and
decreases the measuring accuracy or cause
premature failures.
• If the photometer lamp is stained, perform an End
Process and wait at least 5 minutes. Check that the
photometer lamp has cooled down completely, then
wipe off the stain with a soft cloth dampened with
alcohol.

Instruction
As Needed When removing the lamp unit cover, be careful not to
bump the cover against the sample probe.
Prepare the following:
• New photometer lamp MU9888 3. Remove the lamp unit cover.
1. Check that an End Process was performed and 4. Loosen the two knobs on the terminals, then
allow at least 5 minutes for the lamp to cool disconnect the two lamp cords from the
after system shut down. terminals.
2. Open the upper cover. 5. Loosen the lamp holder by turning it
counterclockwise, then pull the photometer lamp
from the receptacle.

F-46 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 18, 2011
 OLU2038E

Protrusion
Lamp unit cover
Notch

Knobs
Lamp holder

T
ON
Lamp receptacle FR Lamp cords
Guide key
Caution
Collar notch
To obtain optimal analysis data, do not start a photocal
until the photometer lamp has stabilized after starting
Lamp holder the system. The photometer lamp will stabilize
approximately 20 minutes after system start-up.
6. Remove the lamp from the lamp holder and
collar. Insert the new lamp into the collar and 2. The mode changes from Warm Up to Standby
lamp holder. after 20 minutes.

7. Insert the lamp into the lamp receptacle. Be 3. Optional: Perform a photometer check to make
sure to match the notch on the guide key and the sure the lamp was installed properly.
collar notch of a new photometer lamp unit with 4. Perform the Weekly Photocal procedure.
the protrusion on the lamp receptacle.
Caution
Caution After replacing the photometer lamp, be sure to perform
Tighten the lamp holder securely. If the lamp holder is the photocal measurement to obtain accurate data.
loose, accurate analysis data cannot be obtained.

8. Turn the lamp holder clockwise to secure the See Also

photometer lamp. For information about performing a photocal, refer to
the “Weekly Maintenance” section of this chapter.
9. Connect the two lamp cords to each terminal,
then tighten the two knobs. The cords can Tips
connect to either terminal. If errors occur as a result of the photocal measurement,
10. Replace the lamp unit cover. try the following:
• For numerous errors, check that the photometer
11. Close the upper cover. lamp was installed correctly, or the new photometer
lamp may be defective:
A. Install the photometer lamp again.

B. Perform a photometer check.

• For few errors, the photometer lamp was replaced

correctly, but some cuvettes may be stained:

A. Clean the cuvettes that caused the error.

B. If the error is not corrected even after cleaning,

replace the problem cuvettes and perform a new
photocal. Verify that photocal results are in range.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-47
January 28, 2005
 6.6 Clean or Replace Individual Cuvettes
If a cuvette is stained or scratched, a photometric error will result. To obtain accurate analysis
results, replace cuvettes that have stains which cannot be removed by cleaning, or have
scratches. Also, replace cuvettes that have error messages as a result of the photocal, or if
cuvettes cannot pass a photocal even after sonicating them. The following procedure explains
how to replace and clean individual cuvettes.
As Needed 7. To Replace Individual Cuvettes: Insert the
Prepare the following: new cuvette(s) into the wheel. Gently push the
cuvette completely into the wheel or problems
• New cuvette ZM0634 with analysis could occur.
• This procedure requires using Neutrad. The To Clean Individual Cuvettes: Sonicate
wash solution can be ordered by calling 1-800- cuvettes in a 2% Neutrad solution for 15
766-7000. The catalog number is Fisher minutes. If a sonicator is not available, soak
#0435510. The wash solution can be ordered in them in a 5% Neutrad solution overnight. Allow
1 liter or 1 gallon bottles. the cuvettes to dry completely on the outside
• Cotton tipped applicators (overnight or with heat under 50 degrees C.
A hair dryer works well). .
1. Put the analyzer in Standby.
2. Open the rear cover of the analyzer. Instruction
3. Loosen the knob on the wash nozzle unit. Be sure to replace all cuvettes removed from the wheel.
Remove the wash nozzle unit and hang it on the If not, the mixture, reagent, and wash solution will spill
hook. causing an overflow around the wheel. When removing
cuvettes, be careful not to scratch them. Never touch
4. Turn the mix units clockwise approximately 60 the photometric face of a cuvette. If the photometric
degrees by hand to move the mix bars away face is stained in any way the photometric data will be
inaccurate.
from the cuvette wheel.

8. Replace the cuvette wheel cover.

Instruction
When removing the cuvette wheel cover, be careful not 9. Turn the mix bar unit back to its original
to scratch or bump the sample probe, reagent probes, position.
and mix bars.
10. Remove the wash nozzle unit from the hook and
screw it back into position.
5. Gently lift and remove the cuvette wheel cover.
11. Select [Maintenance], [ANL Maintenance].
6. Using two cotton tipped applicators, gently
insert them in the cuvette to be removed and 12. Select "F/Prime Washing-line."
pull up. 13. Press the STAT/Rotation Diag. button. The
wash nozzle tubing is primed with DI water.
14. Repeat the previous step several times until air
is removed.
15. Close the upper cover.
16. Perform another photocal procedure prior to
running the analyzer. All cuvettes must pass the
photocal before analysis can take place.

See Also
For information about how to perform a photocal, refer
to the "Weekly Maintenance" section in this chapter.

F-48 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
6.7 Clean the Cuvettes and the Cuvette Wheel (Part of 6
month PM)
OLP2039E
If the cuvettes and cuvette wheel are not cleaned for an extended period of time, reagents can
build up on the cuvettes and wheel resulting in photometric error. Wash the cuvettes and cuvette
wheel every six months. If a wheel overflow occurs, please refer to “Cleaning Cuvettes and
the Cuvette Wheel after a Cuvette Wheel Overflow” located in the As Needed section of this
OLP2040E

manual.
Mixing bar

Hook

Mixing unit
Cuvette wheel cover
Wash nozzle
station

Knob

FR Cuvette wheel cover Positioning screws

ON
T T
ON
FR
Green positioning mark

As Needed (Part of Six Month PM) screws into the two specified holes shown in the
Prepare the following: drawings on the following page.
• This procedure requires using Neutrad. The 7. Use caution when removing the wheel.
wash solution can be ordered by calling Bumping other devices could cause damage.
1‑800‑766‑7000. The catalog number is Fisher Hold the two screws you placed on the wheel
#0435510. The wash solution can be ordered in and lift.
1 liter or 1 gallon bottles. 8. Turn the wheel upside down and use the reverse
• Cotton tipped applicators end of a cotton tipped applicator to gently push
the cuvette out from the bottom of the cuvette
Removing the cuvette wheel & cuvettes wheel.
1. Put the analyzer in Standby. 9. Remove all 88 cuvettes from the wheel.
Replace any cuvettes that have obvious
2. Open the rear cover of the analyzer.
scratches.
3. Loosen the knob on the wash nozzle unit.
Remove the wash nozzle unit and hang it on the Clean and Replace the Cuvettes
hook. 1. Sonicate the cuvettes in 2% Neutrad solution for
4. Turn the mix units clockwise approximately 60 15 minutes, or soak the cuvette in 5% Neutrad
degrees by hand to move the mix bars away solution overnight.
from the cuvette wheel. 2. Thoroughly rinse the cuvettes in deionized
water, or sonicate them in deionized water for
Instruction 10 minutes to remove any residual detergent.
When removing the cuvette wheel cover, be careful not 3. Allow the cuvettes to dry completely. Use one
to scratch or bump the sample probe, reagent probes,
of the following methods:
and mix bars.
• Allow cuvettes to dry naturally.
5. Gently lift the cuvette wheel cover and remove • Use an oven with the heat set under 50
it. degrees Celsius. (A hair dryer works well.)
6. Remove the two screws on the inner • Use a Kimwipe.
circumference of the cuvette wheel. Tighten the
4. Rinse the cuvette wheel with DI water and dry
thoroughly with a clean cloth.
Automated Chemistry Analyzer
AU400/AU400e User's Guide Chapter F - Maintenance F-49
August 29, 2008
 OLP2042E
Cuvette wheel

Cuvette wheel cover

Cuvette

Hole Reverse side of

Screw cotton tipped
applicator
Hole
Cuvette

Photometric
Yellow positioning mark face
Cuvette wheel
T
ON
FR Green positioning mark
Frosted glass
face

5. Insert the cuvettes back into the wheel. Ensure 9. Turn the mix bar unit back to its original
the cuvette is gently pushed down completely position.
into the wheel, or problems with analysis could 10. Remove the wash nozzle unit from the hook
occur. and screw it back into position.

Instruction Check the system

Be sure to replace all cuvettes removed from the 1. Select [Maintenance], [ANL Maintenance].
wheel back onto the wheel. If not, the mixture,
reagent, detergent, etc., will spill on the wheel causing 2. Select "F/Prime Washing-line."
an overflow. When removing cuvettes, be careful
3. Press the STAT/Rotation Diag button. The
not to scratch them. Never touch the photometric
face of a cuvette. If the photometric face is stained wash nozzle tubing is primed with DI water.
by fingerprints, etc., the photometric data will be 4. Repeat the previous step several times until air
inaccurate.
is removed.
6. Replace the cuvette wheel. Match the 5. Close the upper cover.
positioning pins as shown in the illustration 6. Perform another photocal procedure prior to
below. OLP2043E
running the analyzer. All cuvettes must pass
the photocal before analysis can take place.
Positioning pin on the cuvette
wheel cover

Positioning pins on See Also

the cuvette wheel
For information about how to perform a photocal, refer
to the "Weekly Maintenance" section in this chapter.

Green positioning
mark
T Positioning pin on the
ON
FR cuvette wheel cover

7. Remove the screws and place them in their

original position.
8. Replace the cuvette wheel cover.

F-50 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August 29, 2008
6.7a Clean the Cuvettes and Cuvette Wheel after a Cuvette
Wheel Flood
Erroneous data is generated if the cuvettes become wet on the outside (cuvette wheel overflow).
The reaction of the sample and reagents takes place in a carefully controlled dry incubation bath.
Water on the outside of the cuvette affects the light as it passes through the cuvette. Test results
are impacted due to this change in absorbance. It is important to recognize when an overflow
has occurred, determine the cause for the overflow, and follow the appropriate procedures to
recover from an overflow. For additional information, refer to the Troubleshooting Tab in the
AU400/400e User’s Guide.

As Needed the base so that it does not hit the cuvettes.

Remove the wash nozzle station and hang it on
Prepare the following:
the hook.
• Neutrad (Catalog #0435510 available through
4. Remove any mix bars positioned over the
ThermoFisher at 1-800-766-7000)
cuvette wheel. Pull the mix bars up and out. Be
• Cotton Tipped Applicators careful not to scratch the mix bars.
• #2 Phillips head screwdriver. 5. Gently remove the cuvette wheel cover.

Remove the cuvette wheel & cuvettes Note:

1. Verify that the analyzer is in Warm up or
When removing the cuvette wheel cover, be careful not
Standby. to scratch or bump the sample probe, reagent probe, or
2. Open the large analyzer cover. mix bars.

Caution 6. Locate the 2 large, flat screws on the black plate

in the center of the cuvette wheel.
Ensure the cover is open and secured before
leaning over the wash nozzle station. 7. Pull these two flat screws out of the black plate.
No tools are needed to remove these screws!
These screws are used as “handles” to remove
3. Loosen the knob on the wash nozzle station. the entire cuvette wheel from the incubation
Support the wash nozzle when removing it from bath.

Flat screws

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-50a
August 29, 2008
 Phillips head
screw

Flat screw
Flat screw

Phillips head
screw

8. Attach the flat screws to the cuvette wheel.

Place one of the screws into the opening on
the cuvette wheel in front of cuvette number
1. Place the other screw into the opening on
the cuvette wheel in front of cuvette number
46. Firmly tighten each screw into the cuvette
wheel.
9. Remove the 2 Phillips screws that secure the
cuvette wheel in the incubation bath using a #2
Phillips head screwdriver. These screws are
located in the cuvette wheel in front of cuvette
number 13 and cuvette number 57. Set these
screws aside.
10. Using the “handles” (large, flat screws),
carefully pull the cuvette wheel off of the two
metal positioning pins. It may be necessary to
angle the wheel slightly to clear the mix unit.
11. Place the cuvette wheel upside down on a
Note: protected surface to remove the cuvettes. Use
Do not touch the cuvette window. the reverse end of a cotton- tipped applicator
stick to gently push the cuvettes out of the
wheel. Remove all 88 cuvettes. Exercise
Caution caution during this procedure! The cuvettes may
Use care when removing the cuvette wheel to avoid be scratched when they are removed from the
bumping or scratching other components. cuvette wheel and subsequently will not pass the
photocal.

F-50b Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August 29, 2008
Note: Replacing the Cuvettes and Cuvette Wheel
Do not touch the photometric side of the cuvette. 1. Replace all 88 cuvettes (with the open end
Handle by the frosted edge only! facing up) back into the cuvette wheel.

Note:
Do not touch the photometric side of the cuvette.
Handle by the frosted edge only.

2. Use the “handles” (large, flat screws) to place

the cuvette wheel back into the incubation bath.
It may be necessary to angle the wheel slightly
to clear the mix unit.
3. Match the white alignment indicator on the
cuvette wheel with the white indicator on the
analyzer. If the alignment is correct, the cuvette
wheel can be set onto the two metal positioning
pins.

Clean and Replace the Cuvettes

1. Sonicate the cuvettes in 2% Neutrad solution for
15 minutes, or soak the cuvette in 5% Neutrad
solution overnight.
2. Thoroughly rinse the cuvettes in deionized
water, or sonicate them in deionized water for
10 minutes to remove any residual detergent.
3. Allow the cuvettes to dry completely. Use one
of the following methods:
Cuvette wheel
• Allow cuvettes to dry naturally. removed

• Use an oven with the heat set under 50 Alignment Positioning pin
indicators (one of two pins)
degrees Celsius. (A hair dryer works well.)
• Use a Kimwipe. Cuvette wheel
installed
4. Rinse the cuvette wheel with DI water only. Do
not use any cleaners or detergents as this may
ruin the finish on the metal. Dry the wheel
thoroughly with a lint free cloth.

Note:
In the event of a cuvette wheel overflow, dry the
incubation bath with a lint free cloth. Do not use any
cleaners or detergents to clean the incubation bath.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-50c
August 29, 2008
4. Replace the 2 Phillips screws to secure the Note:
cuvette wheel in the incubation bath. These
Be careful not to scratch the mix bars when inserting
screws are located in the cuvette wheel in front them into mix unit.
of cuvette number 13 and cuvette number 57.
5. Loosen the two “handles” (large, flat screws)
Check the System
and set them in the openings on the black plate
in the center of the cuvette wheel. 1. Select [Maintenance], [ANL Maintenance].

6. Replace the cuvette wheel cover by matching the 2. Select "F/Prime Washing-line."
green dot on the cover with the green dot on the 3. Press the STAT/Rotation Diag button. The wash
analyzer. nozzle tubing is primed with DI water.
7. Remove the wash nozzle station from the hook 4. Repeat the previous step several times until air
and place it on the base. Support the wash is removed.
nozzle station when replacing it on the base so
that it does not hit the cuvettes. Align the holes 5. Close the upper cover.
on the wash nozzle unit over the two positioning 6. Perform the photocal procedure prior to running
screws on the base and tighten the knob. Verify the analyzer. All cuvettes must pass the photocal
that all tubing and tube mounting joints are before analysis can take place.
connected to the nozzles and joint manifolds.
Refer to the “Replace the Wash Nozzle Joint See Also
Tubes” in the As Needed Maintenance section For information about how to perform a photocal, refer
for correct tubing placement in case any lines to the "Weekly Maintenance" section in this chapter.
are disconnected.
8. Replace any mix bars that were removed. Insert
the mix bars into the top of the mix unit. While
inserting the mix bar, rotate it slightly to engage
the flat portion of the mix bar with the gear of
the unit. Do not force the mix bar into the unit!
Damage to the gears may occur.

F-50d Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August 29, 2008
6.8 Perform a W1 Procedure
Perform a W1 (Cuvette Wash) on an as needed basis. The W1 cleans all cuvettes with 2% wash
solution using the wash nozzle unit, and the sample probe with the 2% wash solution in the W1
position on the stat table. The reagent probe and mix bars are cleaned in the wash wells.

As Needed
This procedure takes 9 minutes. View the upper left corner of the screen for procedure progress.
The system counts down the remaining time.
Perform this procedure when either of the following is true:
a. Stop is selected in the middle of a run, and analysis is not started again immediately.
b. Power was lost to the system during analysis, and not recovered in a short period of time.
1. From Standby, select the [System Status] icon.
2. Select function key F5 (W1 Start). The system counts down the remaining time.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-51
January 28, 2005
6.9 Clean Belts and Rack Feed areas
To prevent possible rack jams, clean the following areas using a clean lint free cloth dampened
with water. DO NOT USE ALCOHOL OR DETERGENT.

Rack Buffer Belt

Rack Transport Belts

Rack Collection
Area

Rack Feeder
Belt

Rack Feeder Belt:

1. Wipe the bottom and sides of the Rack Feeder
Belt with a clean lint-free cloth dampened with
water. If it is necessary to move the belt for
ease of cleaning, Select [Maintenance], [Maker
Maintenance], [Anl Diag]. Click the Sample
Set Unit tab at the top of the screen. Select “Q/
As needed Forward” and then press the white, Stat rotation/
Prepare the following: Diag button on the top of the instrument panel.
This advances the belt for ease of cleaning.
• Clean lint-free cloth.
Dry the area with a dry lint-free cloth when
completed.
Rack Transport Belts:
1. Place the analyzer in STOP mode. Rack Buffer Belt:
2. Open the analyzer cover. Remove the 1. Wipe the bottom and sides of the Rack Buffer
unattached dark covers from the rack transport Belt with a clean lint-free cloth dampened with
areas. water.
3. From the Main Menu, Select Maintenance, 2. If it is necessary to move the belt for ease
Maker Maintenance, (A) ANL DIAG, Sample of cleaning, select [Maintenance], [Maker
set Unit tab, Rack Reed, (N) Normal. Maintenance], [Anl Diag]. Click the Mix/
Wheel/Nozzle tab at the top of the screen.
4. Select “Yes” at the start Measure prompt.
Select “T/Forward” and then press the white,
5. Press the Stat Rotation/DIAG button to activate stat rotation/diag button on the top of the
the diagnostic function. instrument panel. This advances the belt
6. To clean, hold a damp cloth to the moving belts. forward for ease of cleaning. Dry the area with
NOTE: Never use alcohol to clean belts. a dry lint-free cloth when completed.

7. After completing the diagnostic function, the Finish

system generates a “3150 No Rack” alarm. 1. Select F2 EXIT to return to the Main Menu.
Clear the alarm.
2. Click the STOP icon or press the STOP/Standby
8. Select Close at the Start screen. key to return the analyzer to the standby mode.
Rack Collection Area: 3. Replace the dark covers over the rack transport
1. Use a clean lint-free cloth dampened with water areas.
to clean the rack collection area. 4. Close the large analyzer cover.

F-52 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 6.10 Clean the Wash Nozzle Unit
The wash nozzle unit is equipped with a total of eight nozzles. The unit consists of six wash
nozzles (three-part nozzle) for mixture aspiration, wash solution/deionized-water (DI-water)
dispensing, and wash solution/DI water overflow aspiration, and one nozzle each for aspiration
and drying. If a nozzle is clogged, the nozzle function is weakened. This results in a cuvette
OLP2085E
wheel overflow or problems with the analysis data. To prevent an overflow and abnormal
analysis data, clean the wash nozzle unit every month. This procedure is also included in the
monthly procedure, Clean the wash nozzle unit, Deionized-Water Tank and Filter, and Sample
Probe Filter.

Tube
Tube mounting
joint

Tube mounting
joint manifold

Knob

Tube mounting Positioning screw

joint manifolds

Wash nozzle
Wash nozzle FR
ON
station T

4. Select “E/Replacing Wash Nozzle.”

5. Press the STAT ROTATION/DIAG button. The
liquid in the tubing on the wash nozzle unit is
drained.
6. Open the rear cover.
7. Remove the four tube mounting joint manifolds
from the wash nozzle unit.

Instruction
• When handling the wash nozzles, be careful not to
scratch them.
• When loosening the knob on the wash nozzle unit
station, do not loosen the positioning screws on
both sides of the knob. These screws are used for
positioning the wash nozzle unit.

As Needed Procedure
8. Loosen the knob on the wash nozzle unit, then
Prepare the following: remove the wash nozzle unit along with the
• Dry, clean cloth (KIMWIPE) tubing.
• Sonicator filled with DI water Clean and replace the Wash nozzle unit
Remove the Wash nozzle unit Caution
1. Open the upper cover. After placing the wash nozzle unit in the sonicator,
2. Verify that the analyzer is in the Warm-up or verify that the tips of the wash nozzle do not rest on the
bottom of the sonicator. When the sonicator is running
Standby Mode. the wash nozzle to vibrate and can damage the tips.
3. Select [Maintenance], [ANL Maintenance].
Automated Chemistry Analyzer
AU400/AU400e User's Guide Chapter F - Maintenance F-53
January 28, 2005
Instruction Caution
• When cleaning the wash nozzle unit using a If O-rings are used without cleaning for an extended
sonicator, be careful not to scratch the wash period of time, or if the cover on the jount unit is closed
nozzles. without any O-ring set in the groove, detergent crystals
• Do not scratch or tear the joints and tubes. A leak will collect and could scratch the O-ring. Check the
may result from the scratched part and the cuvette O-rings during Monthly mainteance. For more reliable
wheel may overflow. maintenance, replace the O-rings once a year.

Tips
The sonicator is the optimal device for cleaning the Instruction
wash nozzles. If a sonicator is not available, use DI
water. While pouring water into the wash nozzles, clean Be sure to attach the tube mounting joint manifolds
each nozzle hole using the supplied stylet. Rinse the in their original places. If a manifold is attached
nozzles in DI water, then dry them with a soft cloth. incorrectly, normal analysis cannot be performed.

9. Clean the wash nozzle unit along with the tubing 13. Attach the manifolds, tighten them firmly.
for 15 minutes, using DI water in a sonicator. When attaching the manifolds, match the color
No wash solution is required. connections and tighten them firmly. For
information about the tube mounting joint
10. Take out the wash nozzle unit from the positions, refer to the drawing on the following
sonicator, then wipe up the water drops using a page.
soft cloth.
14. Match the two positioning holes on the wash
11. Using a gauze dampened with water, wipe any nozzle unit with the positioning screws, then fix
foreign matter from O-rings such as dust or the station by tightening the knob.
detergent residue. If foreign matter remains,
remove the O-ring with tweezers, wash with 15. Close the rear cover.
water, and re-insert.
Check the system
12. If any O-ring is scratched or damaged, replace
1. Select “F/Prime Washing-line” on the
with O-ring MU9638.
[Maintenance], [ANL Maintenance] screen.
2. Press the Stat Rotation/DIAG button. The air
in the tubing of the wash nozzle unit will be
released.
3. Close the upper cover.

OLP2120.eps

Type 1 Type 2

O ring MU9638 O ring MU9638

F-54 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
Tubing Diagram of the Wash Nozzles and Tube Mounting Joints
In order to distinguish individual connecting positions easily, follow the tubing diagram below:

A - Aspirate
B - Overflow
C - Dispense

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-55
January 28, 2005
 6.11 Clean or Replace the Sample Probe Filter
If the sample probe filter is dirty, the system may generate abnormal analysis data. This
procedure is also included in the monthly procedure, Clean the Wash nozzle unit, Deionized-
Water Tank and Filter, and Sample Probe Filter. OLP2024E

Joint
Connecting hose
Button Fixture
Top

Filter case

Button
T Joint
ON
FR Basin

As Needed Procedure 6. Unscrew the case and remove the sample probe
Prepare the following: filter.
• Dry, clean cloth
• Basin
• Sonicator filled with DI water Button
• New water filter ZM3079 (if needed)

Removing the sample probe filter TOP

Instruction Cap
Perform an End Process before performing this procedure.
If this is performed with the sub-power to the system
turned on, water could spill from tubing and tanks. Filter
Positioning
1. Confirm that an End Process was performed. Ring

2. Open the left front cover. Sample Probe

Filter
3. Position a basin to catch any liquid that spills from
the deionized-water drain hose.
4. Pull the Sample Probe filter case forward to O-ring
remove the case from the fixture.
5. While pressing the buttons, disconnect the joints at
each end of the sample probe filter case.
Case
Instruction
Loosen the filter case over the basin. The deionized water
in the filter case will drain from the joint. If water spills
onto the system, immediately wipe it up with a dry, clean
cloth.
When removing the sample probe filter, be careful not to Button
lose the O-ring or the filter positioning ring.

F-56 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 18, 2011
 Sample probe filter

O-ring

Filter case

T
ON OLP2024E
FR

Joint
Connecting hose
Button Fixture

Filter case

Button
T Joint
ON
FR Basin

Cleaning and replacing the Sample Probe

Filter 11. Check for leaks. If no leaks are present, remove
the basin.
1. Clean the Sample Probe Filter for 10 minutes,
using DI water in a sonicator. If a sonicator
Check the system
is not available, rinse in DI water. While
pouring DI water onto the filter, scrub it with a 1. Press the Green power button to apply power to
toothbrush. to the system.

2. If necessary, replace the filter with part number 2. After initialization, select [Maintenance], [ANL
ZM3079. Maintenance].

3. Inspect the O-ring, Filter Positioning Ring, 3. Select "F/Prime Washing-line."

and the inside of the case. Remove any 4. Press the STAT ROTATION/DIAG button.
accumulations with a damp towel.
• Observe the DI-Water and filter tubing. The
4. Rinse the Sample Probe Filter in deionized deionized water will flow through the tube
water. and then the air in the DI-Wiater tubing
5. Insert the Sample Probe Filter into the filter will be released. This prime may need to be
case. performed 2-3 times to ensure that all of the
air is out of the tubing.
6. Position the O-ring correctly on the filter case.
5. Repeat the previous step two or three times
7. Place the Filter Positioning Ring over the filter. until the air in the affected tubing is completely
8. Assemble the top of the filter case and tighten. removed.

9. Refer to the illustrations and verify that the top 6. Close the left front cover and the upper cover.
of the filter case is pointed upward and connect
the two hoses to the filter case fittings. Push
each hose connector into the filter case until a
click is heard.
10. Push the Sample Probe Filter Case into the
metal clip.
Automated Chemistry Analyzer
AU400/AU400e User's Guide Chapter F - Maintenance F-57
January 28, 2005
6.12 Clean the Deionized-Water Tank
Regular cleaning prevents the accumulation of deposits or bacteria that could affect analysis.
This procedure is also included in the monthly procedure, Clean the Wash nozzle unit,
ÊÊOLP2033E
Deionized-Water Tank and Filter, and Sample Probe Filter.

Tips
If low-quality deionized water is obtained from the main water source, the two diluted wash solution tanks may need
to be cleaned in addition to the deionized-water tank. For detailed information, contact Beckman Coulter Technical
Services.

Deionized-water tank Supply

Tubing

Connector Float switch

Fixture

Joint

Filter case Deionized-water

drain hose

T
ON
FR

Basin

As Needed Procedure 6. While pressing the button, disconnect the joint

Prepare the following: of the deionized water drain hose from the tank.

• Dry, clean cloth 7. Pull the DI water tank forward while removing
the tubes inserted in the tank. Lay the tubes in
• Basin the basin
• Freshly prepared 20% bleach 8. Remove the deionized-water tank from the
• Extra DI tank (In start-up kit) instrument.
9. Loosen the water tank cap and remove the float
Instruction switch.
Perform an End Process before performing this 10. Rinse the inside of the tank and tubes with a
procedure. If this procedure is performed with the fresh 20% bleach solution and rinse thoroughly
sub-power to the system turned on, the float switch in
the deionized-water tank will activate and excess water with deionized water.
will drain from the tubing. 11. Let the tank air dry.

Remove and clean the Tank Install the spare tank

1. Confirm that an End Process was performed. 1. Fill the spare DI tank at least half full with DI
water and install the tank. Alternate tanks so
2. Open the left front cover. each one can dry thoroughly between cleaning.
3. Unplug connector #240.
Caution
4. Position a basin under the deionized-water drain
hose on the floor. If the tank is empty, and the pump runs without water,
the pump may be damaged.
5. Pull the Sample Probe Filter Case forward to
remove it from the metal clip.

F-58 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 OLP2034E

Cap

Float switch

T
ON
FR

Deionized-water tank

Tubes

2. Insert the float switch and the water supply Check the system
tubes into the deionized-water tank. 1. Press the Green power button to apply power to
3. Replace the DI-water Tank in the system. Verify to the system.
that the tubing is fully inserted in the DI-water 2. After initialization, select [Maintenance], [ANL
Tank. Maintenance].
4. Reconnect the joints on the deionized-water tank 3. Select "F/Prime Washing-line."
and Sample Probe Filter Case. Push each joint
until a click is heard. 4. Press the STAT ROTATION/DIAG button.

5. Tighten the cap. • Observe the DI-Water and filter tubing. The
deionized water will flow through the tube
6. Reconnect connector #240. When reconnecting and then the air in the DI-Wiater tubing
the #240 connector, do not force the connection will be released. This prime may need to be
or the pins can be damaged. Turn the connector performed 2-3 times to ensure that all of the
gently until it slides into the connection easily air is out of the tubing.
and without force.
5. Repeat the previous step two or three times
7. Push the Sample Probe Filter Case into the until the air in the affected tubing is completely
metal clip. removed.
8. Remove the basin. 6. Close the left front cover and the upper cover.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-59
January 28, 2005
 OLP2023E
6.13 Clean or Replace the Deionized-Water Filter
If the deionized-water filter is dirty, the system may generate abnormal analysis data. This
procedure is also included in the monthly procedure, Clean the Wash nozzle unit, Deionized-
Water Tank and Filter, and Sample Probe Filter.

Water supply tube

Filter case
Basin

Deionized water filter

T
ON
FR Filter case cap

As Needed Procedure 7. Pull the DI water tank forward while removing

Prepare the following: the tubes inserted in the tank. Lay the tubes in
the basin.
• Dry, clean cloth
8. Remove the deionized-water tank from the
• Basin instrument.
• Sonicator filled with DI water
Instruction
Removing the deionized-water filter Loosen the filter case over the basin. The deionized
water in the filter case will drain from the joint. If the
Instruction deionized water spills onto the system, immediately
wipe it off with a dry, clean cloth.
Perform an End Process before performing this
procedure. If this procedure is performed with the sub-
power to the system turned on, the float switch in the 9. Turn the DI-Water Filter case cap to remove it.
deionized-water tank will activate and excess water will
drain from the tubing. 10. Remove the deionized-water filter from the filter
case.
1. Confirm that an End Process was performed.
Cleaning and replacing the deionized-water
2. Open the left front cover. filter
3. Disconnect connector #240. 1. Clean the deionized-water filter for 10 minutes
4. Position a basin to avoid spilling liquid from the in DI water using a sonicator. If a sonicator is
deionized-water drain hose onto the floor. not available, pour DI water over the filter while
scrubbing it with a toothbrush.
5. While pressing the button, disconnect the joint
of the deionized water drain hose from the tank. 2. If necessary, replace the water filter with part
number ZM3079.
6. Pull the Sample Probe filter case forward to
remove it from the metal clip. 3. Rinse the deionized-water filter in deionized
water.

F-60 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 18, 2011
4. Insert the deionized-water filter into the filter Check the system
case. 1. Press the Green power button to apply power to
5. Replace the filter case cap and hand tighten. to the system.
2. After initialization, select [Maintenance], [ANL
Caution Maintenance].
If the tank is empty, and the pump runs without water, 3. Select "F/Prime Washing-line."
the pump may be damaged.
4. Press the STAT ROTATION/DIAG button.
6. Replace the deionized-water tank in the system. • Observe the DI-Water and filter tubing. The
Verify that the tubing is fully inserted in the DI- deionized water will flow through the tube
water Tank. and then the air in the DI-Wiater tubing
7. Connect the joints onto the DI water tank. Push will be released. This prime may need to be
each joint until a click is heard. performed 2-3 times to ensure that all of the
air is out of the tubing.
8. Reconnect connector #240. When reconnecting
the #240 connector, do not force the connection 5. Repeat the previous step two or three times
or the pins can be damaged. Turn the connector until the air in the affected tubing is completely
gently until it slides into the connection easily removed.
and without force. 6. Close the left front cover and the upper cover.
9. Push the Sample Probe Filter Case into the
metal clip.
10. Remove the basin.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-61
January 28, 2005
 OLP20

6.14 Clean or Replace the Static Discharge Brushes

Static discharge brushes reduce the chance of static electricity affecting a sample reading, by
allowing static electricity to discharge before sampling takes place.

Static Discharge Brush Maintenance

When sample tubes or cups are overfilled with sample, the excess sample accumulates on the
static discharge brushes during analysis. This accumulation can eventually lead to a level
detection error or a rack jam. To prevent these errors, users should clean soiled brushes or
replace brushes that are worn or cannot be cleaned.
Cleaning or replacing the Static Discharge Brushes

Prepare the following:

• Latex gloves
• Alcohol prep
• Static Discharge Brush MU8424 (Left side).
Static Discharge Brush MU8423 (Right side)

Note
Part numbers MU8424 and MU8423 include one static discharge brush, order one of each if
replacing both brushes. Analyzers should have two static discharge brushes installed on each side
of the rack transport. If the analyzer is equipped with only one static discharge brush contact
your Field Service Engineer.

Caution
To avoid infection always wear latex gloves to clean or replace the static discharge bushes. If your hands
come in contact with the static discharge brushes, wash the affected area thoroughly.

1. Select the Stop key or icon to place the analyzer in STOP mode.
2. Remove the dark acrylic cover from the rack transport area over the cup detector and
barcode reader area.

F-62 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
March 2, 2007
 Static Discharge
Brush Screws

Flat mounting pin

Tilt bottom of brush out to

remove

3. Loosen the static discharge brush screws by turning them gently counter clockwise until
they stop turning. The screws will not come off the analyzer.
4. Lift the brush assembly out of the instrument by tilting the bottom of the static discharge
brush away from the static brush housing, then, lift it up and out clearing the mounting
hole for the flat mounting pin located on the side of the static discharge screw.
5. Follow the same procedure with the brush assembly on the other side of the rack
transport.
6. Clean any stain on the brushes with an alcohol prep from the base to the end of the bristle
tips.
7. If the static discharge brushes are still stained after cleaning or show signs of wear,
replace them.
8. Dispose of the old brushes in a receptacle for biohazard waste.
9. Reinstall the clean or replaced brushes, making sure the flat mounting pins on the static
discharge housing fit into the mounting holes on the brush assembly.
10. Tighten the brush screws, on each assembly.
11. Replace the dark acrylic cover from the rack transport above the cup detector and barcode
reader area.
12. Select the Stop key or icon to place the analyzer in warm up or standby mode.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-62a
March 2, 2007
F-62b Chapter F - Maintenance
Automated Chemistry Analyzer
AU400/AU400e User's Guide
March 2, 2007
7. ISE Maintenance
Each part on the ISE unit requires periodic maintenance to maintain the performance of the ISE
unit. This chapter gives detailed information about maintenance required for the ISE unit.

Contents

7.1 Daily ISE Maintenance........................................................................................................... F-64

7.1.1 Inspect the ISE Reagent (Buffer) Syringe for Leaks................................................... F-65
7.1.2 ISE Cleaning............................................................................................................... F-66
7.2 Weekly ISE Maintenance........................................................................................................ F-68
7.2.1 Perform a Selectivity Check for the Na/K Electrodes................................................. F-69
7.3 Maintenance Every Two Weeks............................................................................................. F-70
7.3.1 Clean the mix bars, liquid level sensors, sample pot and sample pot tubing. ........... F-71
7.4 ISE Maintenance Required Every Three Months................................................................... F-75
7.4.1 Replace the Mixture and Mid-Standard Pump Roller Tubing..................................... F-76
7.4.2 Replace Valve Tubing................................................................................................. F-78
7.5 As Needed ISE Maintenance................................................................................................. F-80
7.5.1 Replace the Reference Electrode............................................................................... F-81
7.5.2 Add Reference Electrode Solution............................................................................. F-83
7.5.3 Replace Reagents...................................................................................................... F-84
7.5.4 Replace the Na, K, or Cl Electrode............................................................................. F-86
7.5.5 ISE Cleaning Procedure............................................................................................. F-88
7.5.6 Replace the reagent buffer syringe............................................................................. F-88

OLP2069E

ISE Mix unit

Knob

Connector #142

Connector #143

Mount plate
FR
ON
T

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AU400/AU400e User's Guide Chapter F - Maintenance F-63
January 28, 2005
7.1 Daily ISE Maintenance
To obtain accurate results and optimum system performance, be sure to perform the following
daily ISE maintenance procedures. Record maintenance on the schedules located at the
beginning of this chapter. Maintenance items can also be recorded on-line in the [Periodic
Maintenance] screen.

7.1 Daily ISE Maintenance........................................................................................................... F-64

7.1.1 Inspect the ISE Reagent (Buffer) Syringe for Leaks................................................... F-65
7.1.2 ISE Cleaning............................................................................................................... F-66

F-64 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 7.1.1 Inspect the ISE Reagent (Buffer) Syringe for Leaks
The ISE reagent syringe dispenses a very small amount of buffer to the sample pot. The buffer
is used to make the ISE sample dilution. If liquid leaks from the syringe, incorrect amounts
OLP2081E
of buffer will be dispensed, and a problem with ISE analysis will result. Before starting daily
analysis, check the ISE reagent syringe for leaks or condensation in the syringe case.

ISE reagent syringe

Sample syringe Fixing screw
Reagent syringe

Case head
Syringe case

FR
ON
T

ISE Reagent Syringe : Possible leakage locations

Daily Maintenance 5. Visually check for leaks inside the syringe case.
Prepare the following: Replace any damaged component.

• Clean dry cloth If leaking or condensation is present, perform the

following steps:
1. Open the right front cover.
6. Loosen the top and bottom fixing screws and
2. Check the following areas for leaks: bottom of pull the syringe and case forward.
the syringe cases, the case head, syringe case,
area around the fixing screws, and the tubing. 7. Verify that the syringe provides a smooth,
(Also make sure the tubing is not crimped.) resistant pull by pulling on the piston.
8. Turn the syringe case by hand. If the case head
See Also and syringe case are loose, tighten them by
For information about replacing syringes, refer to turning the syringe case clockwise toward the
the "As Needed ISE Maintenance" section in this
chapter. For information about troubleshooting syringe case head.
problems, refer to the Troubleshooting Chapter. 9. Visually check each case head for cracks. If
there are cracks on the case head, replace the
Caution case head.
If your skin comes in contact with liquid, immediately
and thoroughly wash with water. 10. Remove the syringe from the case head and
verify that there is one O-ring, and that it is not
damaged.
Caution 11. Verify that the correct size syringe (reagent
Do not place any body parts in the path of moving syringe) is placed in the appropriate position.
equipment, unless you are certain that the analyzer is in
Stop or Standby and that diagnostic functions are not 12. Close the right front cover.
activated.
13. If the previous steps are not successful,
refer to the Replace Sample and Reagent
3. Check the fixing nut (top) and piston fixing Syringe procedure in the Analyzer As Needed
screw (bottom). Tighten them if necessary. Maintenance section.
4. Verify that the bottom screw is placed securely
against the syringe piston.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-65
January 28, 2005
 7.1.2 ISE Cleaning
After completing daily analysis, be sure to clean the sample pot and electrode lines.
Contamination or inaccurate results may occur if the cleaning cycle is not performed.

ISE Cleaning Solution

Daily Maintenance 6. Press the [System Status] key. The System

Prepare the following: Status screen will appear.

• ISE Cleaning Solution (AUH1019) 7. Select [ISE Status] on the screen. The ISE
Status screen will appear.
• One ISE cup (MU9232)
8. Press function key F5 (ISE unit start) on the
1. Check that the system and the ISE unit are on [ISE Status screen]. The window for selecting
and the system is in Warm-up or Standby. the kind of ISE single operations will appear.

Caution 9. Select "E/Cleaning." The window for confirming

whether to start the process will appear.
If cleaning solution comes into contact with your eyes
or is ingested, immediately consult your MSDS guide. 10. Select “YES.” The system starts cleaning the
sample pot and electrode line. This process
2. Fill the ISE cup with 1mL of the ISE cleaning requires approximately 4 minutes to complete.
solution. 11. After the cleaning cycle is complete, press
3. Remove the small STAT table cover. function key F2 (exit) two times. The system
returns to the Main menu.
4. Rotate the STAT table by pressing the STAT
ROTATION/DIAG button until the "CLEAN"
position on the table can be accessed.
5. Set the sample container in the "CLEAN"
position on the STAT table.

F-66 Chapter F - Maintenance

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AU400/AU400e User's Guide
January 28, 2005
Automated Chemistry Analyzer
AU400/AU400e User's Guide Chapter F - Maintenance F-67
January 28, 2005
7.2 Weekly ISE Maintenance
To obtain accurate results and optimum system performance, be sure to perform the following
weekly ISE maintenance procedures. Record maintenance on the schedules located at the
beginning of this chapter. Maintenance items can also be recorded on-line in the [Periodic
Maintenance] screen.

7.2 Weekly ISE Maintenance........................................................................................................ F-68

7.2.1 Perform a Selectivity Check for the Na/K Electrodes................................................. F-69

F-68 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
7.2.1 Perform a Selectivity Check for the Na/K Electrodes
Na and K are ion-selective electrodes. If the selectivity of electrodes deteriorates, the ISE unit
is affected by ions other than those to be measured and appropriate analysis results will not
be obtained. To check the electrodes for deterioration, check the selectivity of the Na and K
electrodes every week. OLP2066E
STAT table
cover (small)

Weekly Maintenance Sample cup

Prepare the following:
STAT table
• Selectivity Check Solution AUH1018 21
22

U-H
1 2

U-L
3

5
20

S-L
K-
SE

6
L-N
• (The set contains bottles for Na and K.)

a
19

7
S-H
18

8
17

9
CLE W1

16
AN
W2 10
15
11
14

N T 13 12

S
RO

TA
T
E
N
S

D
TA
F

S
E
T
Note

IS
E
P
R
IM
STAT ROTATION/DIAG

E
switch

Before performing this procedure a valid ISE STAT Table Top View
calibration is necessary. 11 12
10 13

9 14

1. Check that the system and the ISE unit are on

W1 W2

15
AN

8
E

and the system is in Warm-up or Standby.

CL

16
7
2. Pour Na and K electrode check solution into

17
6
different sample containers.

S-H

18
5
a
-N
EL

3. Remove the small STAT table cover.

19
K-S
L
S-

4
U-L
U-H
20
3

4. Rotate the STAT table by pressing the STAT 2 21

1 22

ROTATION/DIAG button until the S-H and S-L

positions on the table can be accessed.
13. After completing the check process, select the
5. Place the sample containers filled with each "Result Select" tab. The selectivity check results
check solution on the STAT table. Place the will appear.
Na container in the “S-H” (“SEL-Na”) position.
Place the K container in the “S-L” (“K-SEL”) 14. Check the selectivity data. For abnormal data,
position. the background of the numeric value is displayed
in yellow. The system interprets the following
6. Press the [System Status] key. The System values as abnormal data: A value more than
Status screen will appear. 160 for Na-electrode, a value more than 6.0
7. Select [ISE Status] on the screen. The ISE for K-electrode, and values of zero. If an
Status screen will appear. electrode value is judged abnormal, refer to the
Troubleshooting Chapter.
8. Press function key F5 (ISE unit start) on the
[ISE Status screen]. The window for selecting 15. To remove the concentrated check solution,
the kind of ISE single operations will appear. Perform an M-R Prime 3 times. Select [System
Status], [ISE]. Press F6 (prime) and select M-R
9. Select "D/Check selective." The window for
prime. Press the STAT ROTATION/DIAG button.
confirming whether to start the process will
appear.
See Also
10. Select “YES.” The system starts the selectivity For information on electrode replacement, refer to the
check process for the ISE. This process requires "As Needed ISE Maintenance" section in this chapter.
approximately 3 minutes to complete.
11. Remove the sample containers from the STAT
table. Discard the remaining check solution and
the sample containers.
12. Replace the small STAT table cover.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-69
January 28, 2005
7.3 Maintenance Every Two Weeks
To obtain accurate results and optimum system performance, be sure to perform the following
ISE maintenance procedure every two weeks. Record maintenance on the schedules located at
the beginning of this chapter. Maintenance items can also be recorded on-line in the [Periodic
Maintenance] screen.

7.3 Maintenance Every Two Weeks............................................................................................. F-70

7.3.1 Clean the mix bars, liquid level sensors, sample pot and sample pot tubing. ........... F-71

F-70 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 7.3.1 Clean the mix bars, liquid level sensors, sample pot and sample pot
tubing.
Maintenance Every Two Weeks
Prepare the following:
• ·Alcohol Prep
• Wash Solution (OSR0001) diluted to 1%
• Sonicator

Clean the mix bar and liquid level sensors

1. Verify that the analyzer is in Warm up or Standby.
2. Open the analyzer cover.
3. Select [System Status], [ISE Status], function key F6 Prime.
4. Select “A/Replace Electrode.”
5. To drain the flowcell, press the Stat/Rotation Diag button on top of the instrument panel.

Note:
The AU400 ISE unit automatically primes the mid-standard reagent every hour of inactivity to keep the
electrodes conditioned. Normally this procedure takes 20 minutes and requires removing the sample pot
and tubing. To prevent a spill if the unit is apart for more than one hour, turn off the ISE switch.

6. Open the ISE unit cover.

7. Disconnect the Mix Motor connector #142 and Liquid Level Sensor connector #143.
8. Loosen the knob on the Mix Unit. Lift the unit from the positioning pins.
OLP2069E

ISE Mix unit

Knob

Connector #142

Connector #143

Mount plate
FR
ON
T

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-71
January 28, 2005
 Liquid Level
Sensors

J-Nozzles

Mix Bar

9. Carefully wipe the Mix Bar and Liquid Level Sensors with an alcohol prep using a
downward stroke.
10. Set the Mix Unit aside.

Remove and clean the Sample Pot and tubing

OLP2070E
11. Loosen the retaining knob on top of the Sample Pot. Lift the pot from the peg.

Sample pot

Connector Sample pot

fixing knob

Connector

Mixing unit on the

mount plate

FR
ON
T

12. Hold the Sample Pot and remove the Sample Pot tubing from the inlet of the flowcell.
Remove the Pinch Valve tubing from the Pinch Valve.
Disconnect the Pinch Valve tubing #5 at the Y-connector near the Mixture Aspiration Pump.
The Mixture Aspiration Pump is labeled 3 and 4.
ISE Diagram Pinch valve Pinch valve tubing
Sample Pot
Bypass tubing Y-connector

Mixture Aspiration
Pump
Tubing
T-connector
Flow Cell inlet Flow Cell outlet

F-72 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 13. Using a pipette tip attached to a squeeze bottle or a syringe, fill the Sample Pot tubing,
Bypass tubing, and Pinch Valve tubing with the 1% Wash Solution.
a. Place the pipette tip or syringe inside the bottom of the Sample Pot. Force the wash
solution through the Sample Pot tubing.
b. Place the pipette tip or syringe in the Pinch Valve tubing. Force the wash solution
through the Pinch Valve tubing, T-connector, and Bypass tubing.

Note:
Perform this procedure over a sink. Use caution when flushing the tubing. Position the tubing
downward to avoid splashing the eyes or face.

a. b.

14. Place the Sample Pot and tubing in a beaker containing 1% Wash Solution.
15. Place the beaker in the sonicator (filled with water) and sonicate for 10 minutes.

16. Rinse the Sample Pot with DI water.

a. Place the pipette tip or syringe at the bottom of the sample pot. Force DI water through
the sample pot tubing.
b. Place the pipette tip or syringe in the pinch valve tubing. Force DI water through the
Pinch Valve tubing, line connector, and Bypass tubing. Verify that the lines are flushed
thoroughly.

Note:
Perform this procedure over a sink. Use caution when flushing the tubing. Position the tubing
downward to avoid splashing the eyes or face.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-73
January 28, 2005
 17. Dry the sample pot and tubing.

Re-install the Sample Pot and tubing

1. While holding the Sample Pot, push the Sample Pot Tubing on the inlet of the flowcell.
2. Re-install the Sample Pot. Align the hole on the top of the Sample Pot with the peg and
place the screw in the slit on the opposite side. Tighten the screw.
3. Push the Pinch Valve tubing onto the Y-connector located near the Mixture Aspiration pump.
4. Push the Pinch Valve tubing into the top slot of the Pinch valve.
5. Place the Mix Unit on the two positioning pins. Tighten the knob to secure the unit.
Connect the Mix Motor connector #142 and Liquid Level Sensor connector #143.
Note: The connectors are specifically keyed to fit each plug. To avoid damage to the pins,
do not force a connector into its plug. If the pins are damaged, the Mix Bar will not turn or
the Level Sensors on the Mix Unit will not function properly.

Prime the ISE flowcells and tubing

J-nozzles

E/MID/REF Prime D/Buffer Prime

1. Select “E/MID/REF Prime.” Press the Stat/Rotation Diag button. The unit begins priming
for eight cycles.
2. Observe the Mix Unit J-nozzle. Liquid should flow in a straight line from the J-nozzle into
the center of the Sample Pot.
3. Observe the tubing at the outlet of the flowcell (#6). Verify that all bubbles are removed. If
bubbles are present, check all tubing placement and connections and repeat steps 1 and 2.
Do not calibrate the ISE until all air is removed from the flowcell.
4. Select “D/Buffer Prime”. Press the Stat/Rotation Diag button. The unit begins priming for
eight cycles.
5. Verify that the Sample Pot fills and drains with buffer. Liquid should flow in a straight line
from the J-nozzle into the center of the Sample Pot.
6. Close the ISE cover.
7. Use fresh standards to perform an ISE calibration and verify that a valid slope is obtained.
See Chapter C Basic Operations.

F-74 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 7.4 ISE Maintenance Required Every Three Months
To obtain accurate results and optimum system performance, be sure to perform the following
ISE maintenance procedures every three months. Record maintenance on the schedules
located at the beginning of this chapter. Maintenance items can also be recorded on-line in
the [Periodic Maintenance] screen. These procedures are performed twice a year by your
Field Service Engineer as a part of the preventative maintenance service. The operator is also
responsible for performing these maintenance procedures twice a year.

7.4 ISE Maintenance Required Every Three Months................................................................... F-75

7.4.1 Replace the Mixture and Mid-Standard Pump Roller Tubing..................................... F-76
7.4.2 Replace Valve Tubing................................................................................................. F-78

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-75
January 28, 2005
 OLP2071E
7.4.1 Replace the Mixture and Mid-Standard Pump Roller Tubing
The roller tubing will deteriorate due to pressure from the rolling pump and vibration. If the
roller tubing is not replaced for an extended period of time, it may become flat or worn and
leaks may occur. Replace the roller tubing every three months. This procedure is performed
twice a year by your Field Service Engineer as a part of the preventative maintenance service.
The operator is also responsible for performing this maintenance procedure twice a year.

MID solution roller tube

Waste liquid roller tube

T
ON
FR Rolling pump

Maintenance Every Three Months 5. The MID solution and mixture roller tubes
Prepare the following: are stretched over the rolling pump cylinders.

S
Stretch and lift tubing up and away from the

TA
T
E
N
S

D
• Roller tube MU9623

TA
T
rolling pump. Allow it to rest on top of the

S
E
T
IS
E
cylinders.

P
R
1. Select [System Status], [ISE Status], F6 Prime.

IM
E
6. Unscrew each roller tube connector to remove
NOTE the roller tube.
Remain in the "F6 Prime" menu. 7. Connect a new roller tube. Secure the
connectors at both ends of the tube by screwing
2. Select "A/Replace Electrode." them hand tight.
3. Press the STAT ROTATION/DIAG button.
Liquid will be drained from the tubing. Instruction
3. Open the upper cover. Verify the tubing numbers match the labeled pump
connectors. If connected incorrectly, liquid will not be
4. Open the ISE cover. supplied or drained correctly.

Caution 8. Place the roller tubes on the correct rolling

Be careful not to spill the solution on the roller tube pumps, then match the connectors with the
when replacing the tube. notches in the vicinity of the rolling pump.

Instruction
The roller tubing consists of the MID solution roller
tube and the mixture roller tube. Replace these two
roller tubes at the same time.

F-76 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 OLP2073E

Roller tube connectors

N T
F RO

S
TA
T
E
N
S

D
TA
T

S
E
T
Nos.

IS
E
P
R
IM
E
T
ON Nos.
FR

S
TA
T
E
N
S

D
TA
T
9. Press the STAT ROTATION/DIAG button. The

S
E
T
IS
E
P
two rolling pumps are turned on to move the

R
IM
E
solution in each roller tube. Check that the
liquid is completely aspirated from the sample
pot to the electrode unit. If liquid remains in
the sample pot, check the pinch valve tubing
connection. Refer to the diagram located on the
ISE unit door.
10. Select Close.
11. Close the ISE unit cover.
12. Close the upper cover.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-77
January 28, 2005
7.4.2 Replace Valve Tubing
The tubing positioned in the pinch valve may deteriorate with use over time. Replace the
pinch valve tubing every three months. This procedure is performed twice a year by your
Field Service Engineer as a part of the preventative maintenance service. The operator is also
responsible for performing this maintenance procedure twice a year.

Pinch valve tubing

Pinch valve
4

T
ON
FR

Pinch Valve tubing Pinch valve Pinch valve tubing

Sample Pot #5
Bypass tubing

Mixture Aspiration
Pump
Tubing
#3 & #4
#6
Flow Cell inlet Flow Cell outlet

Maintenance Every Three Months 3. Press the STAT ROTATION/DIAG button.

Prepare the following: Liquid will be drained from the tubing.
• New pinch valve tubing ZM2970 4. Open the upper cover.
1. Select [System Status], [ISE Status], F6 Prime. 5. Open the ISE cover.
6. Disconnect the three ends of the pinch valve
Note tubing. Disconnect the tube ends at positions 4,
Remain in the "F6 Prime" menu. 5, and 6 in the drawing above.
7. Pull the top pinch valve tube to the right (#5
2. Select "A/Replace Electrode." in drawing) until it is removed from the slot.

F-78 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 OLP2110E

Pinch valve tubing

Pinch valve

T
ON
FR

While pressing the green button on top of the and insert the top tube (#5 in the drawing) by
pinch valve, pull the lower tube from the slot pushing it all the way into the slot.
(#6 in the drawing), then release the button.
10. Press the STAT ROTATION/DIAG button.

S
TA
Instruction

T
The two rolling pumps are turned to move the

E
N
S

D
TA
T

S
E
solution in each roller tube. Check that the

T
Do not mount each branch tube in a different groove on

IS
E
liquid is completely aspirated from the sample

P
the pinch valve. If it is mounted backwards, the liquid

R
IM
E
will not be supplied and drained correctly. pot to the electrode unit. If liquid remains in
the sample pot, check the pinch valve tubing
8. Mount a new section of pinch valve tubing connection. Refer to the diagram located on the
by connecting the branches at positions 4, 5, ISE unit door.
and 6, as described above. Connect the longer
11. Select Close.
Y-branched tube at position 5.
12. Close the ISE unit cover.
Note: There is a diagram inside the ISE Unit
cover indicating the correct positions in the 13. Close the upper cover.
pinch valve for tubing 5 and 6.
9. Replace the lower tube (#6 in the drawing) by
pressing the green button on top of the pinch
valve. Make sure the tube is inserted all the
way into the slot. Release the green button

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-79
January 28, 2005
7.5 As Needed ISE Maintenance
This section describes the procedures used to perform as needed maintenance, such as those
for replacing damaged parts and supplying reagents. Record maintenance on the schedules
located at the beginning of this chapter. Maintenance items can also be recorded on-line in the
[Periodic Maintenance] screen.

7.5 As Needed ISE Maintenance................................................................................................. F-80

7.5.1 Replace the Reference Electrode............................................................................... F-81
7.5.2 Add Reference Electrode Solution............................................................................. F-83
7.5.3 Replace Reagents...................................................................................................... F-84
7.5.4 Replace the Na, K, or Cl Electrode............................................................................. F-86
7.5.5 ISE Cleaning Procedure............................................................................................. F-88

F-80 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 7.5.1 Replace the Reference Electrode OLP2076E
Replace the reference electrode, if calibration results are high or low for all 3 electrodes (Na,
K, and Cl), or if calibration results are very unstable. Before replacing the reference electrode:
check the reference packing and replace the internal reference solution. The reference electrode
is under warranty one year or 300,000 samples.

Lock lever
(Electrodes are locked in this state.)
Thermistor connector

Thermistor

REF electrode cord

T
ON
FR

7. Select "A/Replace Electrode."

8. Press the STAT ROTATION/DIAG button.
Liquid that remains in the area of electrodes is

S
TA
drained.

IS
As Needed Maintenance
Prepare the following: Tips
If the STAT ROTATION/DIAG button is pressed again,
• REF electrode MU9197
MID-solution will be supplied to the tube.
• REF electrode packing MU9202
• Internal Reference Solution AUH1017 9. Disconnect all electrode cords. Reference is
green, Na, K, and Cl are color-coded.
Instruction 10. Move the lock lever to unlock the electrodes.
The reference electrode may break if excessive force is 11. Remove the Na, K, and Cl electrodes.
applied when replacing the electrode.
12. Gently lift the block on which the REF electrode
is mounted.
1. Check that the system and the ISE unit are on
and the system is in Warm-up or Standby. Instruction
2. Open the upper cover. When loosening the set screw, do not push or twist the
thermistor cord, otherwise it may break.
3. Open the ISE unit cover.
4. Press the [System Status] key. The System 13. Loosen the set screw, then gently remove the
Status screen appears. REF electrode along with the set screw. Gently
remove the packing with a pair of hemostats.
5. Select [ISE Status] on the screen. The ISE
Status screen appears.
6. Press function key F6 (prime) on the [ISE
Status] screen. The window for selecting prime
operations appears.
Automated Chemistry Analyzer
AU400/AU400e User's Guide Chapter F - Maintenance F-81
January 28, 2005
 Connector

REF electrode
insertion hole
Packing
Set screw
REF electrode Block

T
ON
FR

Cross-sectional View of REF Electrode

This end up
Electrode bar

REF electrode

14. Check that no air bubbles are introduced to the electrodes are properly placed, the lock lever
electrode tip on a new REF electrode. is secured, and the O-rings are in the proper
position.
If air bubbles reside in the electrode tip of
the new REF electrode, remove the bubbles 25. Close the ISE unit cover.
by orienting the electrode bar downward and 26. Close the upper cover.
tapping it with a finger.

S
27. Wait approximately 5 minutes before performing

TA
T
15. Replace the packing in the block.

E
N
S

D
calibration analysis.

TA
T

S
E
T
16. Mount the new REF electrode along with the set

IS
E
P
screw on the block. See Also

R
IM
E
For information about how to perform calibration, refer
17. Tighten the set screw to secure the REF to the Basic Operations Chapter.
electrode.
18. Replace Na, K, and Cl electrodes based on the Caution
color-coded diagram. To obtain the best possible analysis data, perform two
successive calibration measurements to confirm the
19. Remount the block. electrode stability. If the difference between the first
and second calibrations is within the following values,
20.Move the lock lever to lock the electrodes.
the electrodes are stable:
21. Connect all electrode cords. Reference is green; Na 0.020, K 0.045, Cl 0.025
Na, K, Cl are color-coded. If each difference is not within the above values, the
electrode membrane may not be stabilized. Open the
22. Press the STAT ROTATION/DIAG button.
upper cover and the ISE unit cover. Press function key
MID-standard is supplied to the electrodes. F6 (prime) on the [ISE Status] screen. Select mid-prime
23. Select "E/MID/REF Prime" on the [ISE Status] then repeat steps 16 to 20.
screen.
24. Press the STAT ROTATION/DIAG button again.
The two rolling pumps move the solution in
each roller tube. Press the STAT ROTATION/
DIAG button. The rolling pumps activate eight
times. Visually check tube #6 to verify that no
bubbles pass through the electrodes. Repeat this
operation until there are no air bubbles in the
tube. If air bubbles are present, verify that the

F-82 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
7.5.2 Add Reference Electrode Solution
Check the solution inside the REF electrode. It should be above the reference line. If the REF
electrode solution is below the reference line, add more solution.

OLG4085E.AI

REF electrode

Reference line

Internal Reference solution AUH1017

As Needed Maintenance Caution

Prepare the following: The electrode is very fragile. Be careful when adding
solution so the glass does not crack or break.
• Internal Reference Solution AUH1017
1. Remove the cap on the REF electrode.
See Also
2. Add REF electrode solution. If the solution needs to be changed due to calibration
problems, or the electrode requires replacement, refer
3. Replace the cap on the REF electrode.
to the procedure in this chapter called "Replacing the
4. Calibrate the ISE. Reference Electrode."

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-83
January 28, 2005
7.5.3 Replace Reagents
If the reagents used for the ISE unit are insufficient, an alarm message will appear on the screen.
If the alarm message appears, replace the corresponding reagent. After the alarm message
displays, the system will be capable of analyzing approximately 150 samples.
OLP2080EReplace the
reagents before the reagent bottle is empty. The ISE on board reagent stability is specified
below for the buffer solution, Mid-standard solution, and reference solution. Analysis data could
be incorrect if reagents are used beyond the on board stability date.

On board stability
Check your package insert for on board stability claims.

Aspiration tube
MID solution Cap
tank Buffer solution
ISE
tank ON

F
OF

FR
ON
T
REF solution tank

Caution
• Never replace reagents during analysis.
• The density of the REF solution is higher than
those of others. If another solution is mixed with
the REF solution, analysis data will be incorrect.
Never add new reagent to the remaining reagent.

1. Open the front left cover.

2. Pull out the reagent bottle that must be replaced.
3. Unscrew the cap of the reagent bottle, then take
out the aspiration tube.
As Needed Maintenance 4. Place the new bottle on the system. Treat the
Prepare the following: old reagents in the same way as the waste
liquids from the system were treated.
• New buffer solution bottle AUH1011
5. Insert the aspiration tube into the reagent bottle,
• New MID solution bottle AUH1012 then tighten the cap.
• New REF solution bottle AUH1013

F-84 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
Instruction 8. Select [ISE Status].
• Push the reagent bottle in all the way until it stops. 9. Press function key F6 (Prime).
If the reagent bottle is positioned forward, the
liquid-level sensor will not detect the reagent level. 10. Select one of the following and press enter:
This may display an alarm message indicating
D/Buffer Prime (Buffer reagent replaced)
insufficient reagent.
• If the roller tube for the mid-standard is removed E/Mid/Ref Prime (Mid and/or Reference
from the rolling pump, the solution in the tubing reagent replaced)
flows backwards into each reagent bottle. Do not
remove the roller tube for the mid-standard from 11. Press the STAT ROTATION/DIAG button.
the rolling pump when replacing either the mid- Priming lasts approximately 1.5 minutes.
standard or reference.
12. Close the front cover.
6. Push the reagent bottle to the back of the 13. Close the ISE cover.
reagent shelf.
7. Select [System Status].

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter F - Maintenance F-85
January 28, 2005
7.5.4 Replace the Na, K, or Cl Electrode
If the electrodes have deteriorated, appropriate analysis results will not be obtained. Replace
electrodes when calibration results are out-of-range, and troubleshooting has been performed.
Electrodes are under warranty for 40,000 samples or 6 months. Replace them based on
calibration and QC results.
OLP2074

Lock lever (The electrodes

are locked in this state.)

K electrode
Na electrode
Cl electrode

Cord

STAT ROTATION/DIAG switch

S
TA
T
E
N
S

D
TA
T

S
E
T
IS
E
P
R
IM
E
8. Press the STAT ROTATION/DIAG button. The
liquid that remains in the region of electrodes is
drained.

Tips
Select "A/Replace Electrode" and press the STAT
As Needed Maintenance ROTATION/DIAG button once. The liquid in the tube
is drained. If the STAT ROTATION/DIAG button is
Prepare the following: pressed again, Mid-standard solution is supplied to the
tube.
• New Na electrode MU9194
• New K electrode MU9195 9. Disconnect the cords from the Cl, Na, or K
• New Cl electrode MU9196 electrode.

1. Check that the system and the ISE are on and 10. Move the lock lever to unlock the electrodes.
that the system is in Warm-up or Standby. 11. Remove the three electrodes.
2. Open the upper cover.
3. Open the ISE unit cover.
Instruction
A total of four O-rings are attached to one side of three
4. Press the [System Status] key. The System electrodes and to the metal part above the Cl electrode.
Status screen appears. Exercise care so the O-rings are not lost when removing
the electrodes.
5. Select [ISE Status] on the screen, then press
enter. The [ISE Status] screen appears.
6. Press function key F6 (prime) on the [ISE
Status] screen. The window for selecting prime
operations appears.
7. Select "A/Replace Electrode."

F-86 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 28, 2005
 Lock lever

K electrode
Na electrode

Cl electrode

Tube

Blue cord
Yellow cord
Red cord

REF electrode cord

T
ON (Green cord)
FR

12. Mount the new electrode. Replace Na, K, and

Cl electrodes based on color-coded diagram. See Also
For information about how to perform calibration, refer
13. Move the lock lever to lock the electrodes. to the Basic Operations Chapter.
14. Connect the appropriate cord to the
Caution

S
corresponding electrode.

E
N
To obtain the best possible analysis data, perform two

D
TA
T
15. Press the STAT ROTATION/DIAG button. The

S
E
successive calibration measurements to confirm the

T
MID solution is supplied to the tube.

IS
electrode stability. If the difference between the first

E
P
R
IM
and second calibrations is within the following values,

E
16. Select "E/MID/REF Prime" on the [ISE Status] the electrodes are stable:
screen. Na 0.020, K 0.045, Cl 0.025
17. Press the STAT ROTATION/DIAG button. The If each difference is not within the above values, the
rolling pumps activate eight times. Visually electrode membrane may not be stabilized. Open the
check tube #6 to verify that no bubbles pass upper cover and the ISE unit cover. Press function key
F6 (prime) on the [ISE Status] screen. Select mid-prime
through the electrodes. Repeat this operation then repeat steps 16 to 20.
until there are no air bubbles in the tube. If air
bubbles are present, verify that the electrodes
are properly placed, the lock lever is secured,
and the O-rings are in the proper position.
18. Close the ISE unit cover.
19. Close the upper cover.
20. Wait at least 5 minutes before performing
calibration analysis.

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AU400/AU400e User's Guide Chapter F - Maintenance F-87
January 28, 2005
 7.5.5 ISE Cleaning Procedure
To help eliminate sample buildup/residue from high volume sample testing, ISE Cleaning may
be necessary on an as-needed basis. This method should be used when the ISE calibration
slopes are in the mid-to-low forties, or if a build-up/residue is present upon inspection of the
sample pot or T-tubing. This is the ONLY ISE Cleaning Procedure recommended by Beckman
Coulter.

As Needed Maintenance 8. Manually turn the roller pump to clear the

Prepare the following: bleach from the lines.

• 10% bleach Solution (10mL Clorox bleach + 9. Pipette 10mL of ISE Mid-Standard Solution
90mL DI H20) into the sample pot and manually turn the roller
pump to clear the Mid- Standard Solution.
1. Select [System Status], [ISE Status]. Repeat 3-5 times.
2. Select F6/Prime. 10. Replace the mix assembly and the pinch valve
3. Select A)Replace Electrode then press the STAT tubing.
Rotation/DIAG button. The flow cell will drain. 11. Press the STAT Rotation/Diag button on the
4. From the current menu, remove the mix analyzer.
assembly from the sample pot. 12. Perform three to four mid-standard primes.
5. From the current menu, remove both pinch 13. Perform a Total prime then exit the current
valve tubing’s (#5 and #6) from the pinch valve. menu.
6. For the first 2 minutes, pipette the 10% bleach 14. Calibrate the ISE.
solution into the ISE sample pot and manually
turn the left-hand roller pump assembly 15. Run QC Material.
clockwise until most of the bleach empties from
the sample pot into the ISE tubing. Continue
filling the sample pot with the bleach solution
while turning the left roller pump assembly.

Note 7.5.6 Replace the reagent buffer

Do not completely empty the sample pot before adding
syringe
more bleach solution. Ensure the tubing is filled with
the bleach solution.
See Analyzer As-Needed Maintenance, "6..4
7. Let the bleach solution remain in the line for the Replace Sample, Reagent Syringe".
remaining five minutes.

F-88 Chapter F - Maintenance

Automated Chemistry Analyzer
AU400/AU400e User's Guide
March 2, 2007
 Chapter G
Error Flags

Introduction
If an error flag is detected during sample analysis, it is logged in the analysis data. Error flags
are printed to the right of the result. Flags are also displayed on the data display screen. On the
DPR display, data with a flag appears in a different color (red) than data without flags (black).
The following table gives a cause and corrective action for each error flag.

Contents
Introduction...............................................................................................................G-1
1. Checking Results...............................................................................................G-4
2. Data Error Flags.................................................................................................G-5
FLAG: i (Manual dilution not calculated for ISE tests)................................................ G-5
FLAG: d (Flags QC data that was excluded from calculation)..................................... G-5
FLAG: e (Edited Data).................................................................................................. G-5
FLAG: ( (Shortage of detergent for contamination parameters in user defined detergent
bottles R1 & R2)............................................................................................. G-5
FLAG: R (Reagent is empty)......................................................................................... G-5
FLAG: # (Sample Level Detection Error)...................................................................... G-5
FLAG: % (Clot detected)............................................................................................... G-6
FLAG: ? (Unable to perform calculations).................................................................... G-6
FLAG: U (Reagent blank absorbance at last photometric point is low)........................ G-7
FLAG: u (Reagent blank/routine absorbance at first photometric point is low)............ G-7
FLAG: Y (Reagent blank absorbance at last photometric point is high)...................... G-8
FLAG: y (Reagent blank/routine absorbance at first photometric point is high)........... G-8
FLAG: @ (Test results are too high).............................................................................. G-9
FLAG: $ (Not enough data to determine linearity of reaction)...................................... G-9
FLAG: D (Absorbance of reaction is greater than maximum OD range).................... G-10
FLAG: B (Absorbance of reaction is less than minimum OD range).......................... G-10
FLAG: * (Linearity error in rate methods)....................................................................G-11
FLAG: & (Prozone test data is abnormal)....................................................................G-11
FLAG: Z (Prozone error)..............................................................................................G-11
FLAG: ! (Unable to calculate concentration)............................................................. G-12
FLAG: ) (Cannot convert from OD to CONC)............................................................ G-12

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter G - Error Flags G-1
June, 2013
 FLAG: a (Expired reagent or onboard stability expired)............................................. G-12
FLAG: b (Calibration expired)..................................................................................... G-12
FLAG: F (Results higher than the dynamic range)..................................................... G-13
FLAG: G (Results lower than the dynamic range)...................................................... G-13
FLAG: p (Out of the panic value range)..................................................................... G-14
FLAG: T (Abnormality found in inter-chemistry check)............................................... G-14
FLAG: P (Positive: Any value above flag level H)....................................................... G-14
FLAG: N (Negative: Any value below flag level L)...................................................... G-14
FLAG: H (Result higher than normal value range)..................................................... G-14
FLAG: L (Result lower than normal value range)....................................................... G-15
FLAG: J (Result is higher than repeat run range)...................................................... G-15
FLAG: K (Result is lower than repeat run range)....................................................... G-15
FLAG: x (Data not registered).................................................................................... G-15
FLAG: 1 (Data exceeds QC range)............................................................................ G-15
FLAG: 2 (Data exceeds the 3SD control limit)........................................................... G-16
FLAG: 3 (Data continuously exceeds the 2SD control limit)...................................... G-16
FLAG: 4 (Data exceeds R4S control limit).................................................................. G-16
FLAG: 5 (Data exceeds 41S control range)................................................................ G-16
FLAG: 6 (Data over/under last 10 averages)............................................................. G-16
FLAG: 7 (Data over/under range)............................................................................... G-17
FLAG: S (Sample repeated and original results replaced with repeat results)........... G-17
FLAG: / (Test requisition entered, but not performed)............................................... G-17
FLAG: r (Data transmitted to the host computer)...................................................... G-17
FLAG: c (Corrected Data).......................................................................................... G-17
2.1 Troubleshooting for Data Flags ?, @, $, D, F, G, !..................................................................G-18
3. Non-Fatal and Fatal Errors.................................................................................G-21
3.1 Non Fatal Errors (Yellow)........................................................................................................G-22
Cal. error test exist:................................................................................................................ G-22
Cal. expired:........................................................................................................................... G-22
Calibration: No Selected (STAT):.......................................................................................... G-22
Calibration: No Selected (Rack): .......................................................................................... G-22
Cuvette check not performed................................................................................................. G-22
Cuvette temperature:............................................................................................................. G-22
Error cuvette:......................................................................................................................... G-22
Incorrect reagent set:............................................................................................................. G-23
ISE buff. shortage:................................................................................................................. G-23
ISE mid. shortage:................................................................................................................. G-23
ISE no cal.:............................................................................................................................. G-23
ISE not connected:................................................................................................................ G-23
ISE ref. shortage:................................................................................................................... G-23
ISE select errors:................................................................................................................... G-23
ISE slope errors:.................................................................................................................... G-23
ISE stop:................................................................................................................................ G-23
On board expired:.................................................................................................................. G-23
QC: No select (STAT):.......................................................................................................... G-23
RB error test exist:................................................................................................................. G-23
Reagent lot no (cal):.............................................................................................................. G-23
Reagent lot no. (RB):............................................................................................................. G-23
Reagent temperature:............................................................................................................ G-24
STAT table cover open:.......................................................................................................... G-24
Unset reagent:....................................................................................................................... G-24
Unset STAT table:.................................................................................................................. G-24
3.2 Fatal Errors (Red)...................................................................................................................G-25
Diluted washer A short: ........................................................................................................ G-25
Diluted washer B short: ........................................................................................................ G-25
DI water shortage: ................................................................................................................ G-25
Incorrect Parameters: .......................................................................................................... G-25

G-2 Chapter G - Error Flags

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
 ISE Busy: ............................................................................................................................. G-25
ISE cover open: ................................................................................................................... G-25
Rack receiver full: ................................................................................................................ G-25
Reagent cover open: ........................................................................................................... G-25
STAT Table Unchecked: ....................................................................................................... G-26
Unchecked Reagent: ........................................................................................................... G-26
Washer A shortage: .............................................................................................................. G-26
Washer B shortage: ............................................................................................................. G-26
Vacuum tank full: .................................................................................................................. G-26
Other Errors: ........................................................................................................................ G-26

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter G - Error Flags G-3
August, 2002
1. Checking Results
After analysis for one sample finishes, the results are immediately saved on the hard disk and a
list of results are printed.

1. Look at the printout and check it for error flags. If error flags appear on the printout,
perform the corrective action corresponding to that flag. For information on correcting
flags, see the following pages.
2. Perform corrective actions for any alarms generated during the run. For information on
correcting alarms, use the alarm on-line help function.
See Also
For information about checking results, refer to the Basic Operations
OLP4077E
Chapter, "Checking Results.”
Index date/time
System date/time

Index date 1995-11-20 Index date 1995-11-20 Index date 1995-11-20

Index date 1995-11-20 Index date 1995-11-20 Index date 1995-11-20
22:37
Index time 22:37 Index time 22:37 Index time
Operator name Operator name Operator name
OLYMPUS OLYMPUS OLYMPUS

22:37
Report operator name Report operator name Report operator name

Print date
Print time
S.NO. U0005
S.ID
1995-11-21
10:34
sample type urine
Print date
Print time
S.NO. U0006
S.ID
1995-11-21
10:34
sample type urine
Print date
Print time
S.NO. P0001
S.ID
1995-11-21
10:34
Index time 22:37 Index time 22:37 Index time
Operator name Operator name Operator name
Sex Age Month Sex Age Month Sex

Patient information-1 Patient information-1 Patient information-1

Patient information-2 Patient information-2 Patient information-2

Patient information-3

Patient information-4
Patient information-3

Patient information-4
Patient information-3

Patient information-4
OLYMPUS BECKMAN BECKMAN
Report operator name Report operator name Report operator name
Patient information-5 Patient information-5 Patient information-5

Patient information-6 Patient information-6 Patient information-6 ITM01UNI LC

-- 9999.999
Patient comment Patient comment Patient comment ITM02UNI /
-- 9999.999
ITM03UNI /
-- 9999.999
ITM04UNI /
ITM01 0.306 ITM01UNI / ITM01 0.066 ITM01UNI / ITM01 0.03 -- 9999.999
ITM05UNI /
9999.999 - 9998.000 9999.999 - 9998.000 9999.999
-- 9999.999

Print date 1995-11-21 Print date 1995-11-21 Print date 1995-11-21

ITM02 ITM02UNI / ITM02 ITM02UNI / ITM02
-9998.999 -- 9999.999 -9998.999 -- 9999.999 9999.999
ITM03 ITM03UNI / ITM03 ITM03UNI / ITM03
9999.999 - 9998.000 9999.999 - 9998.000 9999.999
ITM04 ITM04UNI / ITM04 ITM04UNI / ITM04

Print time 10:34 Print time 10:34 Print time 10:34

9999.999 -- 9999.999 9999.999 -- 9999.999 9999.999
ITM05 ITM05UNI / ITM05 ITM05UNI / ITM05
9999.999 -- 9999.999 9999.999 -- 9999.999 9999.999

S.NO. U0005 sample type urine S.NO. U0006 sample type urine S.NO. P0001
S.ID S.ID S.ID
Sex Age Month Sex Age Month Sex

Patient information-1 Patient information-1 Patient information-1

Patient information-2 Patient information-2 Patient information-2

Error Flag
Patient information-3 Patient information-3 Patient information-3

Patient information-4 Patient information-4 Patient information-4

Analysis results
Patient information-5 Patient information-5 Patient information-5

Test name Patient information-6

Patient information-6 Patient information-6 ITM01UNI LC
-- 9999.999
Patient comment Patient comment Patient comment ITM02UNI /
-- 9999.999
ITM03UNI /
-- 9999.999
ITM04UNI /
ITM01 0.306 ITM01UNI / ITM01 0.066 ITM01UNI / ITM01 0.03 -- 9999.999
ITM05UNI /
9999.999 - 9998.000 9999.999 - 9998.000 9999.999
-- 9999.999
ITM02 ITM02UNI / ITM02 ITM02UNI / ITM02
-9998.999 -- 9999.999 -9998.999 -- 9999.999 9999.999
ITM03 ITM03UNI / ITM03 ITM03UNI / ITM03
9999.999 - 9998.000 9999.999 - 9998.000 9999.999
ITM04 ITM04UNI / ITM04 ITM04UNI / ITM04
9999.999 -- 9999.999 9999.999 -- 9999.999 9999.999
ITM05 ITM05UNI / ITM05 ITM05UNI / ITM05
Tip 9999.999 -- 9999.999 9999.999 -- 9999.999 9999.999

The system allows two error flags to appear on the data printout per analyte. If more than two events
have occurred, the flags will be listed in priority order. The priority order of all flags are shown on the
table of contents in this chapter.

G-4 Chapter G - Error Flags

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
2. Data Error Flags
FLAG: i (Manual dilution not calculated for ISE tests)
Cause: The i flag appears when ISE tests are requisitioned after a manual dilution rate is
entered in [Routine], [Test Requisition], [Normal]. The manual dilution rate calculation
is not applied to the ISE test results.
Action:
1. To obtain true electrolyte concentrations, multiply the ISE results by the dilution rate.
FLAG: d (Flags QC data that was excluded from calculation)
Cause: This flag is set in [QC Monitor], [Data Edit] by the operator to delete QC data from
statistics.
Action:
1. None.
FLAG: e (Edited Data)
Cause: Data is edited from the [Data Edit] screen.
Action:
1. None.
FLAG: ( (Shortage of detergent for contamination parameters in user defined detergent
bottles R1 & R2)
Cause: The detergent, located in reagent compartment is empty. Reagent positions and
cleaning solution types can be defined in any position. Contamination parameters
will be suspended for the related cleaning solution. Carry-over may have occurred on
affected tests that have this flag.
Action:
1. Fill the cleaning solution bottle in the reagent compartment.
2. Run the affected test(s) for this cleaning solution again.
3. To check contamination parameters, refer to [Parameters], [Special], [Contamination
Parameters].
FLAG: R (Reagent is empty)
Cause: The reagent bottle is empty. This flag is generated when the level detectors fail to
sense reagent.
Action:
1. Add more reagent and repeat analysis. For information on changing reagents, see the
Basic Operations Chapter.
2. Air bubbles may be present in the reagent bottle. Remove the bubbles with a transfer
pipet.
3. Wipe the moisture from the mouth of the bottle.
4. Clean the reagent probe. For information on cleaning probes, see the Maintenance
Chapter.
5. Test the reagent probe for proper operation by replacing it with a new probe.
For information on replacing probes, see the Maintenance Chapter. For detailed
procedures on checking probes, refer to the section in this chapter called "Troubleshooting
for Data Flags."
FLAG: # (Sample Level Detection Error)
Cause: The sample probe failed to detect liquid. This is most commonly caused by: A.
Insufficient quantity of sample within the sample cup. B. Failure of the sample probe
level detector.
Action:
1. Increase the volume of sample within the sample cup and run it again. If the volume of
sample cannot be increased, transfer & run the sample as a priority STAT in a pediatric
cup.
2. Wipe the sample probe with an alcohol prep and verify that the sample probe is properly
attached.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter G - Error Flags G-5
February 1, 2005
 3. Replace the sample probe.
FLAG: % (Clot detected)
Cause: The sample probe detected a clot in the sample.
Action:
1. Do not accept any test results from a sample with a % flag. Some test results from the
sample may NOT be identified with the % flag.
2. Clean the sample probe. Refer to the Maintenance Chapter for cleaning procedures.
3. Re-run the sample that had results with the % error flag.
4. If the error still occurs, replace the sample probe.
FLAG: ? (Unable to perform calculations)
Cause: Data for this sample cannot be calculated due to one of the following:
A. The absorbance of the sample exceeds 2.5.
B. Less than three photometric readings, for a rate reaction, satisfy the assay
criteria specified in the individual test parameters.
C. The Photometric data was defective.
D. The analyzer had a mechanical or electrical malfunction. The samples in
progress, that cannot be completed, will have the “?” flag attached.
Action:
1. The sample may be severely lipemic, icteric, hemolytic or may contain excessively large
amounts of the analyte being tested. Dilute the sample and run it again.
2. Verify the reagent. For more information see “Troubleshooting Reagents and Samples”
located in the Troubleshooting Chapter.
3. The analyzer will generate error codes and/or other mechanical alarms to specify the
malfunction. Once the problem is rectified, run the samples again. For detailed procedures
on checking syringes, probes and calibrator material, refer to the section in this chapter
called, "Troubleshooting for Data Flags ?, @, $, D, F, G, !."
4. If there is no other error flag, repeat analysis after checking the photometer lamp and the
corresponding cuvette. If the system still does not recover from the error contact Beckman
Coulter Technical services.
5. Check the reaction data including those processed immediately before and after the
flagged data result. If any abnormality is found, check the cuvettes and cuvette wash
station for an overflow, then recheck the results processed before and after the flagged data
results. If the issue persists contact Beckman Coulter Technical services.
6. Check the syringes.
7. Check the probes.
8. Verify the calibrator material. Refer to "Troubleshooting for Data Flags" in this chapter for
procedures.

G-6 Chapter G - Error Flags

Automated Chemistry Analyzer
AU400/AU400e User's Guide
November 1 2009
 FLAG: U (Reagent blank absorbance at last photometric point is low)
Cause: The reagent blank absorbance at the last photometric point of the assay is lower than
the lower limit. Most commonly caused by:
• Reagent deterioration: On-board stability date, or reagent expiration date was
exceeded.
• The reagent is in the wrong position within the reagent compartment.
• Invalid reagent OD range parameters.
• Reagent contamination.
• Incorrect reagent preparation.
Action:
1. Verify that the correct settings were programmed in [Parameters], [Specific Test
Parameters].
2. Check reagent integrity. Refer to "Troubleshooting for Data Flags" in this chapter for
procedures.
3. Verify that the reagent is placed in the proper position within the reagent compartment.
Verify that the reagent bottles are in the correct positions.
4. Put on a new bottle of reagent and run again.
5. Verify that the reagent was made properly.
FLAG: u (Reagent blank/routine absorbance at first photometric point is low)
Cause: The reagent blank absorbance at photometer point P0 is lower than the lower limit.
Most commonly caused by:
• Reagent deterioration: On-board stability date, or reagent expiration date was
exceeded.
• The reagent is in the wrong position within the reagent compartment.
• Invalid reagent OD range parameters.
• Reagent contamination.
• Incorrect reagent preparation.
Action:
1. Verify that the correct settings were programmed in [Parameters], [Specific Test
Parameters].
2. Check reagent integrity. Refer to "Troubleshooting for Data Flags" in this chapter for
procedures.
3. Verify that the reagent is placed in the proper position within the reagent compartment.
Verify that the reagent bottles are in the correct positions.
4. Put on a new bottle of reagent and run again.
5. Verify that the reagent was made properly.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter G - Error Flags G-7
August, 2002
 FLAG: Y (Reagent blank absorbance at last photometric point is high)
Cause: The reagent blank absorbance at the last photometric point of the assay is higher than
the high limit defined.
Most commonly caused by:
• Reagent deterioration: On-board stability date, or reagent expiration date was
exceeded.
• The reagent is in the wrong position within the reagent compartment.
• Invalid reagent OD range parameters.
• Reagent contamination.
• Incorrect reagent preparation.
Action:
1. Verify that the correct settings were programmed in [Parameters], [Specific Test
Parameters].
2. Check reagent integrity. Refer to "Troubleshooting for Data Flags" in this chapter for
procedures.
3. Verify that the reagent is placed in the proper position within the reagent compartment.
Verify that the reagent bottles are in the correct positions.
4. Put on a new bottle of reagent and run again.
5. Verify that the reagent was made properly.
FLAG: y (Reagent blank/routine absorbance at first photometric point is high)
Cause: The reagent blank absorbance at photometer point P0 is higher than the high limit
defined.
Most commonly caused by:
• Reagent deterioration: On-board stability date, or reagent expiration date was
• exceeded.
• The reagent is in the wrong position within the reagent compartment.
• Invalid reagent OD range parameters.
• Reagent contamination.
• Incorrect reagent preparation.
Action:
1. Verify that the correct settings were programmed in [Parameters], [Specific Test
Parameters].
2. Check reagent integrity.
3. Verify that the reagent is placed in the proper position within the reagent compartment.
Verify that the reagent bottles are in the correct positions.
4. Put on a new bottle of reagent and run again.
5. Verify that the reagent was made properly.

G-8 Chapter G - Error Flags

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
 FLAG: @ (Test results are too high)
Cause: In dual wavelength measurement, an error occurs if either of the two wavelengths
exceeds 2.5 OD. The probable causes are:
• Specimen quality
• Incorrect placement of reagents within the reagent compartment
• Photometer lamp deterioration
Action:
1. The sample may be severely lipemic, icteric, hemolytic or may contain excessively large
amounts of the analyte being tested. Dilute, then run the sample again or perform a dilution
rerun. If the sample is severely lipemic, perform ultracentrifugal processing on it and then
dilute it.
2. Verify all the reagent positions. An incompatible R1/R2 reagent combination often will
cause absorbancies which exceed the measurable limits.
3. Perform a Photometer Check to verify lamp integrity. If the results are out-of-range, install
a new lamp.

Important: After lamp installation, allow the lamp to stabilize, then a photocal must be
performed. Repeat the Photometer Check with the new lamp installed to verify integrity.
Recalibrate all tests before starting specimen analysis.
4. Verify that the correct settings were programmed in [Parameters], [Specific Test
Parameters].
5. Check the reaction data including those processed immediately before and after the flagged
data result. If any abnormality is found, check the cuvettes and cuvette wash station for an
overflow, then recheck the results processed before and after the flagged data results. If the
issue persists contact Beckman Coulter Technical services.
6. Check syringes.
7. Check probes.
8. Verify calibrator material.
9. Verify reagent integrity and position.

See Also
For detailed procedures on checking syringes, probes and calibrator material, refer to the section in this
chapter called "Troubleshooting for Data Flags ?, @, $, D, F, G, !."
FLAG: $ (Not enough data to determine linearity of reaction)
Cause: Less than three read points of a rate reaction fall within the acceptable OD range
specified. In order to properly calculate a rate reaction, at least three readings must
be taken prior to reaching the maximum or minimum OD limits. If the OD limits are
exceeded, the reaction may have gone into substrate depletion due to either a high
result, or a problem with the integrity of the reagent. The nonlinearity calculations are
not made.
Action:
1. The sample may be severely lipemic, icteric, hemolytic or may contain excessively large
amounts of the analyte being tested. Dilute the sample and run it again.
2. Check syringes.
3. Check probes.
4. Verify calibrator material.
5. Verify reagent integrity and position.
6. Check the reaction data including those processed immediately before and after the flagged
data result. If any abnormality is found, check the cuvettes and cuvette wash station for an
overflow, then recheck the results processed before and after the flagged data results. If the
issue persists contact Beckman Coulter Technical services..

See Also
For detailed procedures on checking syringes, probes and calibrator material, refer to the section in this
chapter called "Troubleshooting for Data Flags ?, @, $, D, F, G, !."

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter G - Error Flags G-9
August, 2002
 FLAG: D (Absorbance of reaction is greater than maximum OD range)
Cause: This flag is generated during a positive reaction or a negative reaction rate method
when the OD of the photometry point FST+2 (first photometry point plus two) exceeds
the OD Value Range Maximum. It is more likely that this flag will occur during a positive
reaction.
OR
In addition, this flag is generated during a fixed method when the OD of a specified read
point (first photometry point or last photometry point) has exceeded the OD Value Range
Maximum.
Action:
1. Verify that the correct settings were programmed in [Parameters], [Specific Test
Parameters].
2. The sample may be severely lipemic, icteric, hemolytic or may contain excessively large
amounts of the analyte being tested. Dilute the sample and run it again or perform a
dilution rerun. If the sample is severely hemolytic, collect the blood again, then repeat
analysis. If the sample indicates an absorbance decrease reaction, it is assumed to be
severely lipemic. If possible perform ultracentrifugal processing on it.
3. Verify reagent integrity and position. Refer to "Troubleshooting for Data Flags" in this
chapter for procedures.
4. If the flag is generated for multiple assays, the lamp may need to be replaced. The integrity
of the lamp can be verified by performing a photometer check. For procedures, refer to the
Maintenance Chapter.
5. Check the reaction data including those processed immediately before and after the flagged
data result. If any abnormality is found, check the cuvettes and cuvette wash station for an
overflow, then recheck the results processed before and after the flagged data results. If the
issue persists contact Beckman Coulter Technical services.
FLAG: B (Absorbance of reaction is less than minimum OD range)
Cause: This flag is generated during a negative reaction or a positive reaction rate method
when the FST+2 photometry point (first specified photometry read point plus two) has an
OD below the OD Value Range Minimum. It is more likely that this flag will occur during a
negative reaction.
OR
When measuring a negative reaction fixed method, the OD of a specified read
point (first photometry point or last photometry point) is below the OD Value Range
Minimum.
Action:
1. Verify that the correct settings were programmed in [Parameters], [Specific Test
Parameters].
2. The sample may be severely lipemic, icteric, hemolytic or may contain excessively large
amounts of the analyte being tested. Dilute the sample and run it again or perform a
dilution rerun.

3. Repeat the sample using a smaller sample volume.

4. Verify reagent integrity and position. Refer to "Troubleshooting for Data Flags" in this
chapter for procedures.
5. If the flag is generated for multiple assays, the lamp may need to be replaced. The integrity
of the lamp can be verified by performing a photometer check.
6. Check the reaction data including those processed immediately before and after the flagged
data result. If any abnormality is found, check the cuvettes and cuvette wash station for an
overflow, then recheck the results processed before and after the flagged data results. If the
issue persists contact Beckman Coulter Technical services.

G-10 Chapter G - Error Flags

Automated Chemistry Analyzer
AU400/AU400e User's Guide
November 1 2009
 5. If the flag is generated for multiple assays, the lamp may need to be replaced. The integrity
of the lamp can be verified by performing a photometer check.
FLAG: * (Linearity error in rate methods)
Cause: This flag is generated when the rate of a reaction is judged to be nonlinear due to
exceeding the defined % variance or OD limits between photometer read points. Possible
causes are:
• Contaminated reagent
• Unusually high result
• Defective cuvettes
• Electrical noise
• Dirty or defective mix bars
• Reagent dispense probe alignment problem
• Sample probe alignment
The acceptable linearity is defined in [Parameters], [Specific Test Parameters], Linearity %.
Action:
1. Verify that the correct settings were programmed in [Parameters] [Specific Test Parameters].
2. Replace reagent if contaminated or expired.
3. Perform troubleshooting or maintenance procedures listed in the bullet points above.
5. If the sample is assumed to be abnormally high, dilute the sample and repeat analysis.
6. Check the reaction data including those processed immediately before and after the flagged
data result. If any abnormality is found, check the cuvettes and cuvette wash station for an
overflow, then recheck the results processed before and after the flagged data results. If the
issue persists contact Beckman Coulter Technical services.
FLAG: & (Prozone test data is abnormal)
Cause: This flag is generated if the data check measurement point OD of the prozone test
data exceeds OD 2.5.
Action:
1. Dilute the sample and run it again or perform a dilution rerun.
FLAG: Z (Prozone error)
Cause: This flag is generated if the data check equation for either logic check 1, 2, or 3 is
satisfied and the check for low concentration passes. This flag commonly occurs if the
sample contains unusually high concentrations of analyte.
Action:
1. Verify that the correct settings were programmed in [Parameters], [Specific Test
Parameters].
2. Dilute the sample and run it again or perform a dilution rerun.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter G - Error Flags G-11
November, 1 2009
 FLAG: ! (Unable to calculate concentration)
Cause: The system has failed to calculate a result.
Action:
1. If this is a single sample issue, repeat and dilute if necessary.
2. If multiple samples are affected, review all operating parameters such as:
• Reagent quality
• Calibration
• Sample integrity
• General system issues
3. Check the reaction data including those processed immediately before and after the flagged
result. In the presence of any abnormality, check the cuvettes for a possible overflow. If the
issue persists, contact Beckman Coulter Technical Services.
4. If the flag is generated on Na, K, or Cl, repeat a sufficient number of samples which
preceded the appearance of the "!" flag in order to verify that no incorrect results were
reported. It is possible that air in the flowcell affected samples before the "!" flag was
generated.
• Perform a MID/REF Prime and verify no bubbles are in the tubing at the bottom of the
flowcell to ensure there are no obstructions in the flowcell path.
• Verify all tubing is properly connected.
See Also
For detailed procedures on checking syringes, probes and calibrator material, refer to the section in this
chapter called "Troubleshooting for Data Flags ?, @, $, D, F, G, !."
FLAG: ) (Cannot convert from OD to CONC)
Cause: A discrepancy between the reagent lot number and the calibrated reagent lot number
was found.
Action:
1. Recalibrate the test which produced the ")" flag.
2. From the main menu select [Routine], [Data Management], [Data Correction]. This screen
is used to calculate results when a calibration fails.
FLAG: a (Expired reagent or onboard stability expired)
Cause: Results using reagents with expired dating or expired on-board stability will be in
concentration units with an "a" error flag attached.
Action:
1. Put on a new bottle of reagent and perform a reagent volume check.
2. The operator can choose to accept the results and remove the flag by selecting [Routine],
[Data Management], [Data Edit]. Select function key F5 (Data Display), then F5 (Edit).
Check the Flag box and delete the "a" flag.
FLAG: b (Calibration expired)
Cause: Results with expired calibration curves will be in concentration units with a "b" error
flag attached.
Action:
1. Perform another calibration.

See Also
For detailed procedures on performing a calibration, refer to the Basic Operations Chapter.
2. The operator can choose to accept the results and remove the flag by selecting [Routine],
[Data Management], [Data Edit]. Select function key F5 (Data Display), then F5 (Edit).
Check the Flag box and delete the "b" flag.

G-12 Chapter G - Error Flags

Automated Chemistry Analyzer
AU400/AU400e User's Guide
June, 2013
 FLAG: F (Results higher than the dynamic range)
Cause: The concentration of the sample has exceeded the Dynamic Range High (linearity of
the reagent) limit.
Action:
1. Verify that the correct settings were programmed in [Parameters], [Specific Test
Parameters].
2. If the parameters are correct, Dilute the sample and run it again. Refer to the package
insert for information on dilutions.
3. Check syringes.
4. Check probes.
5. Verify calibrator material.
6. Verify reagent integrity and position. Refer to "Troubleshooting for Data Flags" in this
chapter for procedures.

See Also
For detailed procedures on checking syringes, probes and calibrator material, refer to the section in this
chapter called "Troubleshooting for Data Flags ?, @, $, D, F, G, !," and Chapter H Troubleshooting.
FLAG: G (Results lower than the dynamic range)
Cause: The concentration of the sample is below the Dynamic Range Low (linearity of the
reagent) limit.
Action:
1. Verify that the correct Dynamic Range limits are programmed in the [Parameters], [Specific
Test Parameters] screen, then repeat analysis.
2. Check syringes.
3. Check probes.
4. Verify calibrator material.
5. Verify reagent integrity and position. Refer to "Troubleshooting for Data Flags" in this
chapter for procedures.
6. Follow laboratory protocol for samples with values less than the Dynamic Range low limit.

See Also
• See Chapter D, "Repeat Specific," in volume 1 of the User's Guide.
• For detailed procedures on checking syringes, probes and calibrator material, refer to the section
in this chapter called "Troubleshooting for Data Flags ?, @, $, D, F, G, !," and Chapter H
Troubleshooting.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter G - Error Flags G-13
November 30, 2006
 FLAG: p (Out of the panic value range)
Cause: The result has exceeded the panic value limits. Limits are set in [Parameters],
[Specific Parameters], "Panic Value".

Note
The AU400/AU400e will generate an alarm in addition to the "p" flag for an ISE test and other tests if
the panic value range is exceeded.

Action:
1. Verify the correct panic range is programmed for your lab.
2. Follow your laboratory protocol for panic results.
FLAG: T (Abnormality found in inter-chemistry check)
Cause: This flag will be attached to the data which exceeds the High/Low limits specified for
the Check Parameters. The check parameters are designed to test the relationship
between different analyte results on the same sample. i.e. Direct Bilirubin should be
less than Total Bilirubin.
Action:
1. Review data. Verify that the correct inter-item range for your lab is programmed.
2. Follow your laboratory protocol for abnormal test results.
FLAG: P (Positive: Any value above flag level H)
Cause: The value is above flag level H in [Specific Test Parameters].
Note: This flag is not a panic value. This flag is used mainly in drug screenings to define the
positive limit of a result.
Action:
1. None.
FLAG: N (Negative: Any value below flag level L)
Cause: The value is below flag level L in [Specific Test Parameters].
Action:
1. None.
FLAG: H (Result higher than normal value range)
Cause: The data has exceeded the High limit on the normal value range. The normal range is
set in [Parameters], [Specific Parameters], "Normal Ranges".
Action:
1. Review data.

G-14 Chapter G - Error Flags

Automated Chemistry Analyzer
AU400/AU400e User's Guide
November 30, 2006
 FLAG: L (Result lower than normal value range)
Cause: The data has exceeded the low limit on the normal value range. The normal range is
set in [Parameters], [Specific Parameters], [Normal Ranges].
Action:
1. Review data.
FLAG: J (Result is higher than repeat run range)
Cause: The result has exceeded the high limit for the repeat run decision level. Repeat
ranges are set in [Parameters], [Repeat Parameters], [Repeat Specific].
Action:
1. Follow laboratory protocol for repeat samples.
FLAG: K (Result is lower than repeat run range)
Cause: The result is below the low limit for the repeat run decision level. Repeat ranges are
set in [Parameters], [Repeat Parameters], [Repeat Specific].
Action:
1. Follow laboratory protocol for repeat samples.
FLAG: x (Data not registered)
Cause: In multi-rule quality control, if one of the two data pairs are out of range, the other
piece of data is marked with this flag.
Action:
1. This indicates that the data is not registered in the stack. Follow laboratory protocol for out-
of-range QC results.
FLAG: 1 (Data exceeds QC range)
Cause: One point of QC data exceeds the control limit determined by +2SD. This is defined
under [Parameters], [QC Control], [QC Common].
Note: This flag will not be generated using multi-rule to QC logic.
Action:
1. Follow laboratory protocol for out-of-range QC results.
2. Refer to the "Quality Control" section located in the Specifications Chapter for corrective
actions.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter G - Error Flags G-15
August, 2002
 FLAG: 2 (Data exceeds the 3SD control limit)
Cause: One point of QC data exceeded the +3SD limit.
Action:
1. Follow laboratory protocol for out-of-range QC results.
2. Refer to the "Quality Control" section located in the Specifications Chapter for corrective
actions.
FLAG: 3 (Data continuously exceeds the 2SD control limit)
Cause: Two continuous (simultaneously high and low) QC data points exceeded the control
limit of +2SD in one direction.
Action:
1. Follow laboratory protocol for out-of-range QC results.
2. Refer to the "Quality Control" section located in the Specifications Chapter for corrective
actions.
FLAG: 4 (Data exceeds R4S control limit)
Cause: Two continuous QC data points exceeded the control limit of +2SD and a total range
of 4SD was exceeded.
Action:
1. Follow laboratory protocol for out-of-range QC results.
2. Refer to the "Quality Control" section located in the Specifications Chapter for corrective
actions.
FLAG: 5 (Data exceeds 41S control range)
Cause: Indicates that four continuous QC data points exceeded the +1SD limit.
Action:
1. Follow laboratory protocol for out-of-range QC results.
2. Refer to the "Quality Control" section located in the Specifications Chapter for corrective
actions.
FLAG: 6 (Data over/under last 10 averages)
Cause: Data for 10 consecutive controls falls on the same side of the mean.
Action:
1. Follow laboratory protocol for out-of-range QC results.
2. Refer to the "Quality Control" section located in the Specifications Chapter for corrective
actions.

G-16 Chapter G - Error Flags

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
 FLAG: 7 (Data over/under range)
Cause: A control trend is indicated. Control data points to an increase or decrease in
succession.
Action:
1. Follow laboratory protocol for out-of-range QC results.
2. Refer to the "Quality Control" section located in the Specifications Chapter for corrective
actions.
FLAG: S (Sample repeated and original results replaced with repeat results)
Cause: A test was repeated, and the repeat result will be the final result.
Action:
1. None.
FLAG: / (Test requisition entered, but not performed)
Cause: The test was not completed even though it was requested. This error is most
commonly caused by a lack of reagent available to complete the test.
Action:
1. Verify that the reagent is placed in the proper position within the reagent compartment.
2. Verify that the reagent bottle contains enough reagent to perform the test. Manually check
the reagent bottle to view its contents. For more information on changing reagents refer to
the Basic Operations Chapter.
FLAG: r (Data transmitted to the host computer)
Cause: The data was transmitted to the host computer.
Action:
1. None.
FLAG: c (Corrected Data)
Cause: Data was corrected in the [Data Correction] screen.
Action:
1. None.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter G - Error Flags G-17
August, 2002
2.1 Troubleshooting for Data Flags ?, @, $, D, F, G, !
Check the Syringes
A. Verify that the top and bottom screws are hand tight. Note: Do not use any mechanical
instrument to tighten the screws.
B. Verify that the bottom screw is tight and flush with the piston.
C. Verify that the syringe provides a smooth and resistant pull.
D. Verify the correct size syringe is in use (reagent or sample).
E. Verify that one, undamaged O-ring is present.
F. Verify the teflon tip is not flaking or worn.
G. Check the syringe tubing for leaks or crimps. Verify that the metal tubing connectors are
on correctly.

Check the Probes

A. Verify that the probes are straight with no occlusions or scratches.
B. Verify the probes are on the analyzer correctly and are not bent.
C. Verify two metal screw caps are on tightly.

Verify the Calibrator Material

A. Check the lot # and expiration date. Check the stability for storage conditions on the
calibrator material (freezer, refrigerator, open bottles).
B. If using a lyophilized calibrator, check the reconstituted stability and verify that it was made
correctly. Use a volumetric pipet to add the diluent.
C. Verify the calibrator material was not left at room temperature for an extended time period.
Avoid prolonged exposure to air before processing, because evaporation will affect analyte
concentration.
D. Verify the calibrator material was not contaminated at any point prior to or during the run.
Check for indications of instability such as abnormal color, turbidity, or a precipitate. Use
fresh material if necessary. Refer to the calibrator package insert.
E. Verify the calibrator material is in the correct rack position. The yellow rack with barcode
#001 identifies calibrator material #1-10 in rack positions #1-10. Yellow rack with barcode
#002 identifies calibrator material #11-20 in rack positions #1-10. The calibrators are
identified by this system for up to 80 calibrators. Place the correct calibrator material in the
corresponding position in the barcoded rack. The Cal. No. (calibrator position in the yellow
rack) assigned to a test can be verified in [Parameters], [Calibration], [Calibration Specific].

G-18 Chapter G - Error Flags

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
Verify the Reagent Integrity
A. Check the lot # and expiration date. Check the stability for storage conditions on the
reagent. Follow the directions on the package insert.
B. Verify the reagent material was not contaminated at any point prior to or during the run.
Check for indications of instability such as abnormal color, turbidity, or a precipitate. Use
fresh material if necessary. Refer to the package insert for preparation instructions.
C. Verify that the reagent is placed in the compartment properly and that the correct
parameters were entered. Refer to the Basic Operations Chapter for procedures on placing
reagents.

Verify the QC material

A. Check the lot # and expiration date. Check the stability for storage conditions on the QC
material. Follow the directions on the package insert.
B. Use a volumetric pipet, if reconstitution is necessary. Eliminate possible errors by preparing
fresh QC material.
C. Avoid prolonged exposure to air before processing, because evaporation will affect analyte
concentration.
D. Verify the QC material was not contaminated at any point prior to or during the run. Check
for indications of instability such as abnormal color, turbidity, or a precipitate. Use fresh
material if necessary. Refer to the QC package insert.
E. Verify the QC material is in the correct rack position.
1. Place the green rack with the control sample on the rack feeder unit. If the control is
barcoded it can be placed in any position on the green rack.
2. Start analysis.
3. While the rack is being analyzed, check that there are no errors in the quality control
data.
4. Using [QC Monitor] from the [Routine] screen, check the daily variation chart and the
day-to-day variation chart for abnormal data.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter G - Error Flags G-19
August, 2002
G-20 Chapter G - Error Flags
Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
3. Non-Fatal and Fatal Errors
This section lists all possible non-fatal errors along with a description of each one.
3.1 Non Fatal Errors (Yellow)........................................................................................................G-22
Cal. error test exist:................................................................................................................ G-22
Cal. expired:........................................................................................................................... G-22
Calibration: No Selected (STAT):.......................................................................................... G-22
Calibration: No Selected (Rack): .......................................................................................... G-22
Cuvette check not performed................................................................................................. G-22
Cuvette temperature:............................................................................................................. G-22
Error cuvette:......................................................................................................................... G-22
Incorrect reagent set:............................................................................................................. G-23
ISE buff. shortage:................................................................................................................. G-23
ISE mid. shortage:................................................................................................................. G-23
ISE no cal.:............................................................................................................................. G-23
ISE not connected:................................................................................................................ G-23
ISE ref. shortage:................................................................................................................... G-23
ISE select errors:................................................................................................................... G-23
ISE slope errors:.................................................................................................................... G-23
ISE stop:................................................................................................................................ G-23
On board expired:.................................................................................................................. G-23
QC: No select (STAT):.......................................................................................................... G-23
RB error test exist:................................................................................................................. G-23
Reagent lot no (cal):.............................................................................................................. G-23
Reagent lot no. (RB):............................................................................................................. G-23
Reagent temperature:............................................................................................................ G-24
STAT table cover open:.......................................................................................................... G-24
Unset reagent:....................................................................................................................... G-24
Unset STAT table:.................................................................................................................. G-24
3.2 Fatal Errors (Red)...................................................................................................................G-25
Diluted washer A short: ........................................................................................................ G-25
Diluted washer B short: ........................................................................................................ G-25
DI water shortage: ................................................................................................................ G-25
Incorrect Parameters: .......................................................................................................... G-25
ISE Busy: ............................................................................................................................. G-25
ISE cover open: ................................................................................................................... G-25
Rack receiver full: ................................................................................................................ G-25
Reagent cover open: ........................................................................................................... G-25
STAT Table Unchecked: ....................................................................................................... G-26
Unchecked Reagent: ........................................................................................................... G-26
Washer A shortage: .............................................................................................................. G-26
Washer B shortage: ............................................................................................................. G-26
Vacuum tank full: .................................................................................................................. G-26
Other Errors: ........................................................................................................................ G-26

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AU400/AU400e User's Guide Chapter G - Error Flags G-21
June 16, 2006
3.1 Non Fatal Errors (Yellow)
Non fatal errors can occur during start-up. The following section gives more information on these
errors and how to correct them. Non fatal means the operator can choose to start analysis anyway.
If the field called "Check Non Fatal Error" located in [System Maintenance] is not checked, only
fatal errors will appear when the STAT buttonOLP1020
is pressed.

?
STANDBY 1997/09/03 10:10

(R) Routine (P) Parameters (A) Auxiliary (M)Maintenance (U) User (L) Logout

(S)Start Condition
(T)Test Requisition Errors found as below.Start Measure?
(R)Reaction Monitor Yes No
(C)Calibration Monitor
(P)Photocal Monitor
(Q)QC Monitor Serum Urine Others
(D)Data Management
Start Routine S.No.:
(L)Data Report
Operator Name:

Change Select Check Start No

ALARMCLEAR

See Also
For start-up procedures, refer to the Basic Operations Chapter.

Cal. error test exist:

A new test was added to the system and a calibration was not performed or failed the calibration.
Check the test located in [Routine], [Calibration Monitor], [Calibration Curve] screen. Refer to
"Troubleshooting for Data Flags" in this chapter for procedures on verifying calibrator material.

Cal. expired:
Calibration frequency has expired. Check the calibration stability date in [System Status], [Reagent
Status]. Requisition for the expired tests in the [Routine], [Test Requisition], [Calibration] screen.
Run the reagent blank and calibrate the expired tests.

Calibration: No Selected (STAT):

The STAT table is set for manual requisition and calibrator material is on the STAT table, but no
requisition was performed. Requisition calibrator.

Calibration: No Selected (Rack):

No tests are requisitioned for calibration in [Routine], [Test Requisition], [Calibration]. If
calibration is required, requisition for calibration before starting analysis. If calibration is not
required, start analysis.

Cuvette check not performed

Following a Photocal, the results were not checked. Select F5 (Check Start ) in [System Status]
[Cuvette Status].

Cuvette temperature:
Temperature has exceeded standards. Call Beckman Coulter Technical Services.

Error cuvette:
A cuvette may be dirty or scratched. Check the error in [System Status], [Cuvette. Status]. Refer
to the Maintenance Chapter for procedures on cleaning and/or replacing cuvettes. Perform a
photocal. Also refer to the Maintenance Chapter for procedures on performing a photocal.

Automated Chemistry Analyzer

G-22 Chapter G - Error Flags AU400/AU400e User's Guide
June 16, 2006
 Incorrect reagent set:
Check the error in [System Status], [Reagent Status]. This may mean: the calibration stability
expired, the reagent is empty, the on-board stability expired, or the date on the reagent bottle
expired.

ISE buff. shortage:

Insufficient buffer solution.

ISE mid. shortage:

Insufficient mid-standard solution.

ISE no cal.:
1. The ISE was not calibrated for serum or urine. OR 2. An End Process was performed and
"ISE no cal" occurs again when the instrument is on. You can start analysis and use the last
calibration slopes obtained.

ISE not connected:

The main ISE Power Switch is off. Turn on the ISE Power Switch.

ISE ref. shortage:

Insufficient reference solution.

ISE select errors:

Refer to the Selectivity Check procedure in the ISE Maintenance section of the Maintenance
Chapter.

ISE slope errors:

Refer to the ISE Calibration Errors procedure in the Troubleshooting Chapter.

ISE stop:
The ISE unit has stopped. Reset the ISE unit in [System Status], [ISE Status].

On board expired:
Reagent on-board stability has expired. Select [System Status], [Reagent Status] and check the
on-board stability, if it has expired, replace the reagent. Perform a reagent volume check to
update the information.

QC: No select (STAT):

The STAT table is set for manual requisition and QC material is on the STAT table, but no
requisition was performed. Requisition QC.

RB error test exist:

A new test was added to the system and a reagent blank was not performed or failed. Check
the test located in [Routine], [Calibration Monitor], [Reagent Blank Monitor] screen. Refer to
"Troubleshooting for Data Flags" in this chapter for procedures on verifying reagent integrity.

Reagent lot no (cal):

A different reagent lot number from that used at calibration was placed on the analyzer.
Calibrate the analyzer again.

Reagent lot no. (RB):

A different reagent lot number from that used for the reagent blank was placed on the analyzer.
Perform another reagent blank.

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AU400/AU400e User's Guide Chapter G - Error Flags G-23
June 16, 2006
 Reagent temperature:
Reagent refrigerator temperature is out of the standard range. Call Beckman Coulter Technical
Services.

STAT table cover open:

The STAT table cover is not on the table or is not placed properly.

Unset reagent:
A reagent assigned to the round in use (on-board) is missing from the refrigerator. Determine
what reagent is missing. Use [Reagent Status], function key F7 (Test Display) and compare with
the reagents in the refrigerator. Perform a reagent volume check.

Unset STAT table:

A STAT table check is performed and calibration or QC cups assigned to positions in
[Parameters] are not present on the table. When performing calibration or QC analysis, place
cups in position and use the [STAT Table Status] screen to assign the positions in the software.

Automated Chemistry Analyzer

G-24 Chapter G - Error Flags AU400/AU400e User's Guide
June 16, 2006
3.2 Fatal Errors (Red)
Fatal errors can occur during start-up. The following section gives more information on these
errors and how to correct them. Fatal means the operator cannot start analysis until the problem
is corrected. OLP1020

?
STANDBY 1997/09/03 10:10

(R) Routine (P) Parameters (A) Auxiliary (M)Maintenance (U) User (L) Logout

(S)Start Condition
(T)Test Requisition Errors found as below.Start Measure?
(R)Reaction Monitor Yes No
(C)Calibration Monitor
(P)Photocal Monitor
(Q)QC Monitor Serum Urine Others
(D)Data Management
Start Routine S.No.:
(L)Data Report
Operator Name:

Change Select Check Start No

ALARMCLEAR

See Also
For start-up procedures, refer to the Basic Operations Chapter.

Diluted washer A short:

The solution in diluted detergent tank A is low.

Diluted washer B short:

The solution in diluted detergent tank B is low.

DI water shortage:
The deionized water tank is low.

Incorrect Parameters:
A message appears on screen indicating the incorrect parameter. Open the screen containing
the incorrect parameter, determine the parameter error and correct it. The parameters are saved
automatically.

ISE Busy:
The ISE unit is operating. After the ISE operation is complete start analysis.

ISE cover open:

The ISE cover is open or it is not closed properly.

Rack receiver full:

The rack receiver unit is full.

Reagent cover open:

The cover to the reagent refrigerator is open.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter G - Error Flags G-25
August, 2002
 STAT Table Unchecked:
1. The large STAT table cover was removed, or 2. A STAT Table check was not done in the
[STAT Table Status] screen. OR 3. Certain [Parameter] screens were accessed. This changes
the status of the STAT Table from "checked" to "unchecked." Perform a STAT Table check in
the [System Status], [STAT Table Status] screen.

Unchecked Reagent:
A reagent check was not performed or the [Parameter] menu was accessed. This will change
the reagent status from "checked" to "unchecked." Select the [System Status], [Reagent Status]
screen and perform a reset.

Washer A shortage:
Replenish fluid in concentrated detergent tank A.

Washer B shortage:
Replenish fluid in concentrated detergent tank B.

Vacuum tank full:

The vacuum tank is full. Wait until the analyzer empties the tank. If the problem persists,
contact Beckman Coulter Technical Services.

Other Errors:
A specific error name and description displays. Clear the error and restart analysis.

G-26 Chapter G - Error Flags

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
Automated Chemistry Analyzer
AU400/AU400e User's Guide Chapter G - Error Flags G-27
August, 2002
 Chapter H
Troubleshooting

Introduction
This Chapter is designed for the operator to obtain quick solutions to common problems
that could occur with the AU400. The following pages provide information on general
troubleshooting procedures for the analyzer and the ISE unit. Keep a history of problems and
corrective actions that occur with the instrument. This helps correct a problem quickly, should it
become necessary to contact Beckman Coulter Technical Services.

Chapter H Troubleshooting
Introduction...............................................................................................................H-1
To Begin Troubleshooting, Answer the Following Questions............................................................H-3
1. Troubleshooting Using On-Line Help...............................................................H-5
2. Troubleshooting the Analyzer...........................................................................H-7
2.1 Data Problems..........................................................................................................................H-8
2.1.1 Checking Abnormal Data in the Software Screens.......................................................H-9
2.1.2 Troubleshooting Software........................................................................................... H-11
2.1.3 Troubleshooting Reagents and Samples....................................................................H-13
2.1.4 Troubleshooting Mechanical Problems.......................................................................H-16
2.2 System Problems....................................................................................................................H-21
2.3 Data Processor Problems.......................................................................................................H-25
2.4 Recovering from an Emergency Stop or Power Loss.............................................................H-28
2.5 Recovering from a Cuvette Wheel Overflow..........................................................................H-29
2.5.1 What Causes a Overflow?..........................................................................................H-29
2.5.2 Recognizing a Overflow..............................................................................................H-29
2.5.3 Recovering from a Overflow......................................................................................H-30
2.5.4 After the Overflow Problem Is Fixed...........................................................................H-30

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-1
November1, 2009
 3. Troubleshooting the ISE .................................................................................H-31
To Begin Troubleshooting the ISE, Answer the Following Questions.....................................H-32
3.1 ISE Sample Requirements...................................................................................................H-34
3.2 Dispensing System................................................................................................................H-35
3.2.1 Sample Probe and ISE Reagent Syringe...................................................................H-36
3.2.2 Pump Tubing:..............................................................................................................H-37
3.2.3 Mix Bar........................................................................................................................H-38
3.2.4 Sample Pot.................................................................................................................H-39
3.2.5 Pinch Valve Tubing.....................................................................................................H-40
3.3 Measuring Components........................................................................................................H-41
3.3.1 O-rings........................................................................................................................H-42
3.3.2 Electrodes...................................................................................................................H-43
3.3.3 Mixture Pump Tubing..................................................................................................H-45
3.3.4 Thermistor...................................................................................................................H-46
3.3.5 Flowcell Block.............................................................................................................H-47
3.4 Calibration Errors...................................................................................................................H-48
3.5 Selectivity Check....................................................................................................................H-50
3.6 Sequential Sample Measure................................................................................................H-51
3.6.1 Shifts & Trends...........................................................................................................H-52
3.6.2 How to Check Reagent Integrity.................................................................................H-54

H-2 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
To Begin Troubleshooting, Answer the Following Questions
For Data Problems

Did you interpret the data printout correctly?

See Also
For information about how to interpret the data printout, refer to the Basic Operations Chapter.

Is the data flagged?

See Also
For information about data flags, refer to the Error Flags Chapter.

Is the calibration out-of-range?

See Also
For information about how to perform a calibration, refer to the Basic Operations Chapter.

Is QC out-of-range?

See Also
For information about how to perform a QC, refer to the Basic Operations Chapter.

Is data erratic? (Have you performed scheduled maintenance?)

See Also
For information about schedule maintenance, refer to the Maintenance Chapter.

For Alarms
An alarm usually indicates a mechanical problem or a communication failure. Follow the
corrective action provided in the on-line help function.

See Also
For information about how to use on-line help, refer to "Troubleshooting Using On-line Help" located in
this chapter.

For an Analyzer Mechanical Error

Usually a mechanical error is associated with an alarm. Follow the corrective action provided in
the on-line help function.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-3
April 24, 2004
For Other Operational Integrity Problems
Were start-up procedures performed correctly?

See Also
For information on start-up procedures, refer to the Basic Operations Chapter.
Is the reagent stable?

See Also
For information on checking reagent stability, refer to the "Troubleshooting for Data Flags" procedure in
the Error Flags Chapter.
Is there enough reagent in the bottles?
Select the [System Status] icon. Select [Reagent Status]. Press function key F5 (check start).

See Also
For information on reagents, refer to "Changing Reagents” in the Basic Operations Chapter.
Has the reagent, calibrator, and QC material been properly stored?

See Also
For information on proper storage, refer to the package insert. The "Troubleshooting for Data
Flags" procedure in the Error Flags Chapter also provides general information on handling these
materials.
Has the analyzer been calibrated correctly?

See Also
For information about performing calibrations, refer to "Performing Calibrations” in the Basic
Operations Chapter.
Has scheduled maintenance been performed?

See Also
For information about schedule maintenance, refer to the Maintenance Chapter.

Has routine component replacement been performed?

See Also
For information about scheduled maintenance, refer to the Maintenance Chapter.

H-4 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
1. Troubleshooting Using On-Line Help
OLP1047
The AU400 is equipped with an on-line help function. The operator can easily obtain
information on alarms, operations and software menus.

Alarm Help icon

Operation Help icon

?
STANDBY 1997/09/03 10:10

(R) Routine (P) Parameters (A) Auxiliary (M) Maintenance (U) User (L) Logout

Menu Help function key

Help

ALARMCLEAR

Alarm Help
Displays information about system alarms. Press the alarm icon (as shown in the illustration
above) while the alarm message is displayed. If using the mouse, move the pointer onto the
alarm help icon, then press the left mouse button.
1. The following information will be displayed on alarm help:
• Alarm Number – An alarm number is associated with each alarm.
• Alarm Name – Name of alarm that occurred on the system.
• System Status – Describes the current state of the system or how the system
responds to the alarm.
• User Action – Provides steps to correct the problem and resume normal operations.
• Cause – Explains why the error occurred.
• Additional Information – Provides more corrective actions for advanced operators
or engineers. Do not perform these functions unless you are qualified!
2. Follow the corrective actions provided on the screen. If the alarm is still generated after
performing all recommended procedures, contact Beckman Coulter Technical Services.
3. To close the help window, select the Exit Help bookmark in the bookmark column or use
the mouse to move the pointer onto the “X” enclosed in a box located at the top of the
screen, then press the left mouse button.
Menu Help
Describes the purpose of the specific software screen and how to use it. Select a screen
that you would like to know more about, press function key F1. If using the mouse, move
the pointer onto the menu help icon that indicates function key F1 then click the left mouse
button.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-5
April 24, 2004
Operation Help
Provides the same procedures found in the User's Guide. This is an on-line guide that can
be searched using Acrobat Reader.
Window help
If the help window is displayed, use the scroll bar to view the entire message.

?
STANDBY 1997/09/03 10:10

Report Format
Common Sample Results(Fix) Results(Seq.) Comment Line

Operation Help

Analyzer Alarm

An error has occurred in the analyzer system.

Contact a service engineer.

Help Exit Set Image Format Print Report No.

ALARMCLEAR

H-6 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
2. Troubleshooting the Analyzer
This section provides troubleshooting information on the analyzer for data, system, and data
processor problems. Procedures for recovering from a power loss, emergency stop, and cuvette
wheel flood are also provided. Performing scheduled maintenance greatly reduces the chances
of problems occurring with the analyzer. For more information about maintenance for each
system component, refer to the Maintenance Chapter.

Contents

2.1 Data Problems..........................................................................................................................H-8

2.1.1 Checking Abnormal Data in the Software Screens.......................................................H-9
2.1.2 Troubleshooting Software........................................................................................... H-11
2.1.3 Troubleshooting Reagents and Samples....................................................................H-13
2.1.4 Troubleshooting Mechanical Problems.......................................................................H-16
2.2 System Problems....................................................................................................................H-21
2.3 Data Processor Problems.......................................................................................................H-25
2.4 Recovering from an Emergency Stop or Power Loss.............................................................H-28
2.5 Recovering from a Cuvette Wheel Overflow..........................................................................H-29
2.5.1 What Causes a Overflow?..........................................................................................H-29
2.5.2 Recognizing a Overflow..............................................................................................H-29
2.5.3 Recovering from a Overflow......................................................................................H-30
2.5.4 After the Overflow Problem Is Fixed...........................................................................H-30

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-7
November1, 2009
2.1 Data Problems
Before performing detailed troubleshooting procedures, answer the following questions:

Did you interpret the data printout correctly?

See Also
For information about how to interpret the data printout, refer to "Checking Results" located in the Basic
Operations Chapter.

Is the data flagged?

See Also
For information about data flags, refer to the Error Flags Chapter.

Is the calibration out-of-range?

See Also
For information about how to perform a calibration, refer to the Basic Operations Chapter.

Is QC out-of-range?

See Also
For information about how to perform a QC, refer to the Basic Operations Chapter.

Is data erratic? (Have you performed scheduled maintenance?)

See Also
For information about schedule maintenance, refer to the Maintenance Chapter.

H-8 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
2.1.1 Checking Abnormal Data in the Software Screens
To analyze abnormal data use the following screens:
[Routine], [QC Monitor], [Daily Control]
[Routine], [QC Monitor], [Day-to-Day Control]
[Routine], [QC Monitor], [Twin Plot]
[Routine], [Reaction Monitor]
[Routine], [Calibration Monitor], [Calibration Curve]
[Routine], [Calibration Monitor], [Calibration Trace]
[Routine], [Calibration Monitor], [Reagent Blank Monitor]
[Routine], [Photocal Monitor]
[Auxiliary], [Data Statistics]
[Auxiliary], [Histogram]

Use the [QC Monitor] Screen

Compare the test with normal QC data and identify the differences. QC parameters are set in the
following screen: [Parameters], [QC Parameters]. Is the error systematic or random?

See Also
For information about the [QC Monitor] screen, refer to the Software Chapter.

Check Error Flags

Review the error flag definition. Check the data again with respect to each error flag definition.

See Also
For information about error flags, refer to the Error Flags Chapter and the "Quality Control" section in
the Specifications Chapter.

Use the [Reaction Monitor] Screen

Use the [Reaction Monitor] screen to compare the normal data with the abnormal data. What
differences exist between the normal and abnormal data?

See Also
For more information about the [Reaction Monitor] screen, refer to the Software Chapter.

Use the [Calibration Monitor] Screen

Use the [Calibration Monitor] screen to compare normal calibration data with abnormal
calibration data. What differences exist between the normal and abnormal calibration data?

See Also
For more information about the [Calibration Monitor] screen, refer to the Software Chapter.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-9
April 24, 2004
Use the [Photocal Monitor] Screen
Use the [Photocal Monitor] screen to check for abnormal cuvettes. The photocal procedure
verifies the integrity of the cuvettes. Cuvettes that are dirty or contain scratches will not pass
the photocal procedure. Cuvettes that fail the photocal procedure could affect analysis results, so
they need to be cleaned or replaced.

See Also
For information about the [Photocal Monitor] screen, refer to the Software Chapter. For information
about how to perform a photocal, refer to the Maintenance Chapter.

Use the [Data Statistics] Screen

After verifying the data, use the [Data Statistics] screen to obtain the mean, SD, CV, and range.

See Also
For information about the [Data Statistics] screen, refer to the Software Chapter.

Use the [Histogram] Screen

After verifying the data, use the [Histogram] screen to check the dispersion of the data.

See Also
For information about the [Histogram] screen, refer to the Software Chapter.

H-10 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
2.1.2 Troubleshooting Software
Verify parameter settings and the measurement data. Troubleshoot abnormal data by checking
the items shown in the figure below:
OLP4068E

A data error affects Check the relation

all the samples for a Verify parameters. between the analysis
specific analysis test. condition and symptom.
Reagent position
Test order
Verify Abnormal data is Verify the Sample volume
measurement found in some measurement Reagent volume
data. test. data again. Wavelength
Photometric point
Abnormal data is Analysis method
found in all tests. END
RATE
FIXED

Verify the calibration data

and reagent blank values.

Check the reaction progress.

Check the reaction curves.
Check the integrity of the cuvettes.
Check the integrity of the mix bars
Check the sample & reagent integrity
.

Verify the photocal

measurement values.

Check Patient Data

If abnormal data is recognized:
• In a single test: If abnormal data is found in a single test or some tests, verify parameters.
• In some tests: If abnormal data is found in some tests, compare the parameters of the tests
with the abnormal data to identify a common parameter(s).
• In all tests: If abnormal data is found in all patient results for a specific test, check the
parameters for that test.

Check Patient Data Again

If the cause of abnormal data cannot be determined after checking parameters, try to determine
if the problem occurs at certain intervals during testing. Does the problem occur after a specific
reagent is used? (May indicate cross-contamination of reagents) Do the patient samples have
something in common? Was a certain anticoagulant used?

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-11
April 24, 2004
Check the Calibration and Reagent Blank
Check whether the calibration or reagent blank could be causing the abnormal data.
If abnormal data is found in a single test:
Compare normal calibration data with abnormal calibration data to identify the difference
between them using the [Calibration Monitor] screen. Also check the reagent blank and
calibration parameters.

See Also
For information about the [Calibration Monitor] screen, refer to the Software Chapter.
If abnormal data is found in some tests:
Identify the same tests that include abnormal data between calibrators. If all abnormalities
are derived from the same calibrator, the calibrator may be the cause of the abnormal data.
Identify if the reagent position for the tests in question are related.

See Also
For detailed procedures on verifying calibrator material, refer to the Error Flags Chapter.
If abnormal data is found in all tests:
There is a high possibility that the calibration analysis itself may result in abnormal data.
Check the mix bars, R1 probe/syringe, sample probe/syringe, water, calibration material and
common hardware.

See Also
For detailed procedures on checking the syringes, checking the probes and verifying calibrator material,
refer to the Maintenance Chapter and Flags Chapter.

Check the Reaction

When a single test is performing erratically, identify where the error occurred in the data using
the [Reaction Monitor] screen.

See Also
For information about how to check reaction progress, refer to the [Reaction Monitor] screen in the
Software Chapter.

Check Photocal Measurement Data

Check the photocal measurement data to identify an abnormality with cuvettes or a photometer
using the [Photocal Monitor] screen.

See Also
For information about how to check reaction progress, refer to the [Photocal Monitor] screen in the
Software Chapter.

Check Photometer Check Data

Use to identify lamp problems. Check to see if the lamp intensity is within the acceptable range.

See Also
For information about photometer lamp replacement, refer to the Maintenance Chapter.

H-12 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
2.1.3 Troubleshooting Reagents and Samples
Reagents or samples may cause abnormal data. The following list describes some reagent and
sample problems that can affect results.

Sample Problems Causing Abnormal Data

The following two items most commonly affect data:
1. Sample evaporation: High results may occur due to evaporation of the sample. Properly
store and tightly cap samples if analysis is delayed.
2. Did not follow package insert instructions: Follow package insert instructions.
Please make note of the following sample requirements:
• This system is designed to analyze serum, urine, and cerebrospinal fluid samples.
If problems are encountered when analyzing a specific test or when using a specific
reagent, consult the package insert, reagent manufacturer, or distributor.
• Use serum that is sufficiently separated from blood clots and urine that is free from
suspended matter or the probe may be clogged and adverse affects on analysis may
result.
• Fibrin suspended in the serum may clog the probe.
• Check that the blood is sufficiently coagulated prior to serum separation. Remove
the suspended fibrin before placing serum on the system.
• If any suspended matter appears in the urine to be dispensed, perform centrifugal
separation to precipitate the suspended matter before testing the urine specimen.
• If a sample requires pretreatment depending on the analysis test, consult the reagent
manufacturer or distributor.

Instruction
See the package insert provided by the reagent manufacturer for a list of acceptable anticoagulants.

• A minimum quantity of sample is required for analysis. Set up an appropriate

quantity of sample, for correct sampling in the system, according to this guide.

See Also
For information about sampling, refer to "Sampling Specifications" in the Specifications Chapter.
• To prevent sample evaporation, do not leave samples unsealed for an extended
period of time. If samples evaporate, correct analysis cannot be obtained.
• The serum is hemolyzed, lipemic, or icteric (LIH). Check the serum for the
extent of LIH. See "Specific Test Parameters" in the Software Chapter for more
information.
• Liquid-level sensor malfunction due to bubbles on the serum or urine surface.
Remove the bubbles from the surface and repeat analysis.
• The sample cups and racks were not placed properly on the system. Place the
sample cups and racks properly on the system.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-13
June 16, 2006
 See Also
For information about placing the sample cups and racks, refer to the Basic Operations Chapter.

See Also
For more information on sample requirements refer to the following: "NCCLS document
(GP16-A), Urinalysis and Collection, Transportation, and Preservation of Urine Specimens:
Approved Guideline," and "College of American Pathologists document, Patient Preparation &
Specimen Handling, Chemistry/Clinical Microscopy (Fascicle VI)."

Reagent Problems Causing Abnormal Data

• The parameters for reagents and samples are not accurate:
Check the parameters settings.
• To analyze serum, urine, or other samples using this system, use the appropriate
reagent. For information about which products to use, consult the reagent
manufacturer, the distributor, or Beckman Coulter Technical Services.
• The methods used to store the reagents, reference materials, and control serums are
described in each package insert. Observe the instructions. If reagents, reference
materials, and control serums are stored improperly, results will be inaccurate even
if they are used within effective periods.
• Consult the package insert or reagent manufacturer or distributor for the stability of
the unsealed product.
• To place the reagent on the system, follow the instructions in the package insert and
in this User's Guide. Unless the reagent is placed properly, accurate results cannot
be obtained and damage to the system could occur.
• Reagent interference between analysis tests: If a reagent is contaminated with
another reagent during analysis, results may be affected. The actual degree of
interference differs depending on the reagent. For detailed information, contact the
reagent manufacturer or distributor. For information on how to check for cross-
contamination between tests, contact Beckman Coulter Technical Services.
• The reagent was not prepared correctly. Replace the reagent. Refer to the package
insert for preparation instructions.
• The reagent is expired. Replace the reagent.

See Also
For information about setting or replacing reagent bottles, refer to the Basic Operations Chapter.
• The liquid-level sensor malfunctioned during reagent aspiration due to bubbles in
the reagent bottle. Remove the bubbles in the reagent bottle.

H-14 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
June 16, 2006
 • Fresh reagent was added to used reagent. Replace the reagent. Do not add used
reagent to fresh reagent.
• General Reagent Troubleshooting. Verify reagents are in the correct positions.
Check the on-board stability.

QC & Calibrator Problems Causing Abnormal Data

• General QC and Calibrator Troubleshooting.
• Verify the correct material is in the correct position in the rack.
• Verify the material was made correctly.
• Check the open bottle date and expiration dates.
• Verify the material was not exposed to air for an extended period of time.

Abnormal Data Caused by Detergent

• The recommended detergent was not used. Contact Beckman Coulter Technical Services.

Other Causes of Abnormal Data

• Periodic maintenance was not performed at the specified period. Be sure to perform
periodic maintenance at the specified period.

See Also
For detailed information about maintenance, refer to the Maintenance Chapter.
• Insecticide was used in the vicinity of the system.
Insecticides can affect the cholinesterase (CHE) levels. If contamination is suspected,
replace the sample cups, reagents, and reagent bottles with new ones. Also wash the
sample probes, reagent probes, mix bars and cuvettes. Never use insecticide in the
vicinity of the system.

See Also
For detailed information about maintenance, refer to the Maintenance Chapter.
• Water purity, electrical specifications, environmental conditions Check major system
specifications, located in the Specifications Chapter.

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AU400/AU400e User's Guide Chapter H - Troubleshooting H-15
April 24, 2004
2.1.4 Troubleshooting Mechanical Problems
Abnormal data may also be caused by hardware malfunctions. Possible causes and remedies for
abnormal data due to defects in hardware or accessories are described below:

Syringe(s) Problems
• Water may leak from the syringes. Hand tighten the syringe cases and case heads of the
sample and reagent syringes.
• General Syringe Troubleshooting.
• Verify the top and bottom screws are hand tight.
• Verify that probes are not clogged.
• Verify the bottom screw is tight up against the piston.
• Verify there is a smooth, resistant pull.
• Verify the correct size syringe is in use (reagent or sample).
• Verify there is one O-ring being used, and that it is not damaged.
• Verify the syringe is on the analyzer correctly.
• Check the syringe tubing for crimps or leaks.
• Check the teflon tip of the syringe for wear.
• Bubbles were generated in the syringe tubing
Select [ANL Maintenance] from the [Maintenance] menu. After selecting “F/Prime
Washing-line” press the STAT ROTATION/DIAG Switch. Air will be removed from the
tubing.
• The syringe tubing is clogged.
Remove the relay tubes, then clean each tube inside using a stylet. If the syringe tubing
remains clogged after cleaning, contact Beckman Coulter Technical Services.

See Also
For information about syringe cleaning and replacement, refer to the Maintenance Chapter.

Probe Problems
• Leaks from the sample probe and reagent probe due to loose probe connectors. Remove the
covers on the sample probe and reagent probe, then tighten the probe connectors.
• Leaks from the dispense tubing Remove the covers on the sample probe and reagent probe,
then tighten the probe connectors. If this does not work, contact Beckman Coulter Technical
Services.
• A clogged sample or reagent probe. Drain the DI water from the sample probe or reagent
probe and check the way the DI water drains.
• The sample probe tip or reagent probe tip was bent or deformed. Replace the damaged
sample probe or reagent probe.
• The sample aspiration position of the sample probe is incorrect. The sample probe tip
is not positioned at the center of the sample cup. Visually inspect the sample probe for
abnormalities. If the sample probe is bent, replace it. If the sample aspiration position is
abnormal and the sample probe is not bent, contact Beckman Coulter Technical Services.

H-16 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
 • The reagent aspiration position of the reagent probe is incorrect. The reagent probe tip
touches the reagent aspiration hole in the reagent refrigerator. Visually inspect the reagent
probe for abnormalities. If the reagent probe is bent, replace it. If the reagent aspiration
position is abnormal and the reagent probe is not bent, contact Beckman Coulter Technical
Services.
• The sample or reagent probe is not aligned over the cuvette. The sample or reagent probe
tip comes into contact with cuvettes. Visually inspect the sample or reagent probe for
abnormalities. If the probe is bent, replace it. If the probe is not aligned properly and it is
not bent, contact Beckman Coulter Technical Services.
• The sample or reagent probe wash position was abnormal. The sample or reagent probe tip
came into contact with each wash well. Visually check the sample or reagent probe for bent
sections. If either of the probes is bent, replace it. If the probe wash position is abnormal
although the sample or reagent probe is not bent, contact Beckman Coulter Technical
Services.
• General Probe Troubleshooting.
• Verify water can be dispensed in a straight stream.
• Verify that the two metal cap screws for the probe connections are tight.
• Verify that the tubing for the probes is free of air bubbles.

See Also
For information about how to replace the sample probe or reagent probe, refer to the Maintenance
Chapter.

Mix Bar Problems

• The mix bars were contaminated.
Wash the mix bars.

See Also
For information about cleaning the mix bars, refer to the Maintenance Chapter.
• The coating on mix bar was removed.
Replace the mix bar.

See Also
For information about how to replace mix bars, refer to the Maintenance Chapter.
• While the mix bars are rotating, abnormal sounds such as rubbing, gear contact noise, or
other abnormal operating sounds were generated.
Contact Beckman Coulter Technical Services.
• The mix bar mounting position was abnormal. The mix bars came into contact with the mix
bar wash well and/or cuvettes.
Contact Beckman Coulter Technical Services.
• The wash water and detergent are not properly drained from the mix bar wash well.
Contact Beckman Coulter Technical Services.
• Mix bars were not properly installed on the unit and the sample and reagents were not mixed
properly.
Install the mix bars again.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-17
April 24, 2004
 See Also
For information about mounting mix bars, refer to the Maintenance Chapter.

Cuvette Wheel or Wash Nozzles Problems

Scratches, fingerprints, or foreign matter is noticed on the cuvettes, or the cuvettes were
stained.

See Also
For information about washing or replacing cuvettes, refer to the Maintenance Chapter.
Moisture is detected outside the cuvette(s) and the cuvette wheel.

See Also
For information about cuvette wheel floods, refer to "Recovering from a Cuvette Wheel Flood" in this
chapter.
The wash water and/or detergent spills from the wash nozzles because proper draining
did not occur, or the nozzles did not aspirate correctly.
Are the tube joints on the wash nozzles loose? Tighten the loose tube joints.
The wash nozzles may be clogged. Clean the wash nozzles.

See Also
For information about how to clean the wash nozzles, refer to the Maintenance Chapter.
After cleaning cuvettes, a large amount of water remains in the cuvettes.
Are the tube joints on the wash nozzles loose. Tighten the loose tube joints.
The wash nozzles may be clogged. Clean the wash nozzles.
• The tube in the concentrated detergent tank floats.
Straighten the tube, and insert it toward the bottom of the tank so that it does not come
into contact with the opening.
• The float switch in the concentrated detergent tank or diluted detergent tank malfunctioned.
Check the float switch connector. Do not bring the tube in the tank into contact with
the float switch. Check that the float switch is not directly subjected to detergent from
the tube. If the float switch malfunction is not corrected by the above action, the switch
must be replaced. Contact Beckman Coulter Technical Services.

See Also
For information about cleaning cuvettes, refer to the Maintenance Chapter.
For information about cuvettes replacement, refer to the Maintenance Chapter.
For information about cuvette wheel floods, refer to the Troubleshooting Chapter.

Photometer Lamp or Photometer Unit Problems

• The photometer lamp has deteriorated.
• The photometer lamp needs to be replaced when:
1. The check falls outside of the acceptable range.
2. Reagent blank flags are generated (U, u, Y, y) for numerous assays.
3. Data is inaccurate.
Perform a photometer check and analyze results. If the lamp is changed, a photocal
must be performed.
See Also

H-18 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
 For information about photocal measurement results, refer to the Software Chapter.
The photometer lamp does not stay lit constantly.
• Perform the photocal measurement 2 times to check the difference between 2 sets of
measurement data. If there is a discrepancy between the two sets, the photometer lamp may
be defective. Replace the lamp and repeat the photocal.

See Also
For information about replacing the photometer lamp, refer to the Maintenance Chapter. Always perform
a photocal after changing the photometer lamp.

Deionized Water Tank Problems

• The deionized water tank is dirty and stained. Water scale has formed on the inside of
the tank. There are particles inside deionized water tank.
• Clean the deionized water tank.

See Also
For information about cleaning the deionized water tank, refer to the Maintenance Chapter.
• After cleaning the deionized water tank, detergent remains.
• Clean the deionized water tank again. Rinse thoroughly with deionized water.
• Verify laboratory deionized water meets specifications.

See Also
For information about water specifications, refer to the Specifications Chapter.

Deionized Water or Dirty Filter Problems

• If Ca, Mg and Fe data are abnormal, the DI water conductivity may be greater than 2.0
us/cm.
• Clean the deionizer. For detailed information, contact Beckman Coulter Technical
Services.
• Tap water below 5 degrees C was used.
• Supply tap water to the deionizer that is greater than 5 degrees C. For detailed
information, contact Beckman Coulter Technical Services.
• The filters are stained, clogged, or have mildew on them.
• Clean the deionized water filter and the sample probe filter. If abnormal data is not
corrected after cleaning, replace the filters.

See Also
For information about how to clean or replace the deionized water filter or sample probe filter, refer to
the Maintenance Chapter.

Incubation Temperature Problems

• The cuvette wheel was removed for an extended period of time and analysis was started
immediately after the wheel was replaced on the system.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-19
April 24, 2004
 • If the cuvette wheel is removed for an extended period, allow one hour or more
after replacing it before starting analysis.

Reagent Refrigerator Problems

• The reagent refrigerator temperature is out-of-range.
• Press the [System Status] key to display the [System Status] screen. On the screen,
check the temperature in the reagent refrigerator.
• Open the reagent refrigerator cover and check that the reagent bottles are cool.

Rack Problems
• General Rack Troubleshooting.
• Check barcode placement.
• Verify sample position according to rack color.
• Verify the correct number of magnets are present in the bottom of the rack.
• Verify the rack was loaded correctly.

See Also
For information about racks, refer to the Basic Operations Chapter.

STAT Table Problems

• The STAT table temperature is out-of-range.
• Make sure the two STAT table covers are on.
• Make sure the covers are placed properly.
• If STAT samples are placed and removed from the table frequently, and the covers
are not kept in place, the temperature will increase.

H-20 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
2.2 System Problems
This section addresses problems that may be caused by malfunctioning hardware.

TEMP REF HIGH Alarm for the Cooling Unit

There is a problem in the reagent storage refrigeration unit. Select on-line alarm help for
detailed corrective actions.

Abnormal Sound Coming from Inside the System

• Air bubbles are trapped in tubing.
• Check the deionized water filter. If the filter is damaged, replace it.

See Also
For information about replacing the deionized-water filter, refer to the Maintenance Chapter.
• Other causes
• The circulation pump, radiator fan, lamp cooling fan, air pump, 24V power supply
fan, or drying pump may be defective.
• Contact Beckman Coulter Technical Services.

Water Supply Tank "Dilution Empty" Alarm

OLP2117
Select on-line help for detailed corrective actions.

Leaks in the Detergent Rolling Pump

Rolling tubes

Detergent rolling
pumps

Connectors

Relay tubes
NT
FRO

• Any rolling tube may deteriorate.

• Check the rolling tubes for cracks. If the tubes are deteriorating, replace them.

See Also
For information about rolling tube replacement, refer to the Maintenance Chapter.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-21
April 24, 2004
 • Are the tube connectors loose?
• Check the connectors. If they are loose, tighten them firmly.

Barcode Errors
• The barcode labels on the sample cups or reagent bottles may be stained.
• Check for water drops or stains on the barcode labels. If water drops are found,
wipe them off. If a sample ID label is stained or damaged, replace it with a new
one. If a reagent ID label is damaged, the reagent position can be fixed so the
barcode will not be used.
• Barcode labels on sample cups or reagent bottles may have fallen off or were not placed
properly. Refer to the Basic Operations Chapter for proper placement procedures.
• The barcode reader may be stained.
• Wipe the window using an alcohol prep.

See Also
For information about placing barcodes, refer to the Basic Operations Chapter.

Leaks Coming from the Bottom of the Analyzer

• The wash line may be obstructed.
• Check for obstructions in the wash wells for the sample probe, reagent probe, etc.
If they are clogged, clean them.

See Also
For information about how to clean the wells, refer to the Maintenance Chapter.
• The waste line may be obstructed or it was installed improperly.
• Does the waste line meet the system specifications?
• Can you see any obstruction in the lines? If so, contact Beckman Coulter Technical
Services.

No Detergent Supplied to the Mix Bars

• The deionized water filter may be clogged.
• Inspect the deionized water filter. If the filter surface is dirty, the filter may be
clogged. Clean the deionized water filter.

See Also
For information about how to clean the deionized water filter, refer to the Maintenance Chapter.

Reagent Alarm when Sufficient Reagent Remains in the Bottles

Select on-line alarm help for detailed corrective actions.

Sample Alarm when Sufficient Sample Remains

Select on-line alarm help for detailed corrective actions.

Detecting No Sample Cup when it is Present

• An unspecified sample cup was used.
• Check if the specified sample cups are used for each rack.

H-22 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
Detecting No Sample Cup when it is Present on the STAT Table
• The related test was not selected during the requisition operation, or the requisition setup
information did not meet the sample cup position on the STAT table.
• Repeat the requisition operation.
• The wrong size adaptor was used for the sample cup set on the STAT table.
• Check if the sample cup is set properly for the STAT table. If it is not, set the
sample cup again using an adaptor appropriate for the sample cup diameter.
• An unspecified sample cup was used.
• Check if the sample cups specified by the STAT table are used. If they are not, use
the specified sample cups.

Printer is not printing or light is not on.

• The power to the printer was not turned on.
• Check that the printer is turned on.
• The printer ribbon is old or missing.
• Replace the ribbon.
• Make sure the on-line button is on.
• Make sure the paper is loaded properly.
• Secure the printer cables.

See Also
For information about how to replace the ribbon, refer to the printer manual supplied with the printer.

Liquid spills from the Reagent Probe Tip

• The reagent probe was not properly installed.
• Select [ANL Maintenance] from the [Maintenance] screen. After selecting “B/
Replace R Probe & Syringe," press the STAT ROTATION/DIAG Switch. DI water
will be drained from the reagent probe tip. Check if the DI water drains normally.
If the DI water does not drain normally, the reagent probe may not be properly
attached. Check reagent probe installation again.
• The reagent syringe case head fixing screw may be loose.
• If the syringe case head fixing screw is loose, tighten it.

Reagent Probe not Aligned over the Cuvette

•
The reagent probe may be bent.
• Check if the reagent probe is bent. If so, replace the reagent probe.

See Also
For information about how to replace the reagent probe, refer to Maintenance Chapter.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-23
April 24, 2004
Abnormal Data Flag # (Sample Level Detection Error) displayed in the Second Half of the
Sample Dispense Operation
• The sample volume is insufficient.
• Check the sample volume.

See Also
For information about error flags, refer to the Error Flags Chapter. For information about sample
volume, refer to "Sampling Specifications" in the Specifications Chapter.

Cuvette Detergent Overflow

• The detergent aspiration nozzles may be clogged.
• Clean aspiration nozzle using a stylet. If the problem is not resolved after cleaning
the nozzle, contact Beckman Coulter Technical Services.

See Also
For information about cleaning nozzles, refer to the Maintenance Chapter.

Sample Rack Jams

• Foreign matter, such as tape, may be on the rack.
• Check for foreign matter on the rack and remove it.
• Foreign matter may be on the bottom of the rack.
• Check if foreign matter, such as a magnet, is incorrectly placed or jammed onto the
bottom of the rack and remove it.
• Clean belts and belt area with deionized water to remove spills or sticky material.

TEMP DIL Alarm for the Wash Heat Unit

Select on-line alarm help for detailed corrective actions.

Printer Problems (Alarm "Part of data is not output yet.")

• Analysis was started while the printer was off-line or turned off.
• During analysis the printer was taken off-line or turned off.
• The printer is out of paper.
1. Turn ON the power to the printer and make sure it is on-line.
2. Load printer paper if necessary.
3. Press the [System Status] key.
4. Select "DPR Status" on the [System Status] screen.
5. Press function key F4 (Printer Control).
6. Select "Resume." The printer will begin printing the data that has been analyzed.
After completing the printout, the system moves to the Standby mode.

H-24 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
2.3 Data Processor Problems
This section provides information on correcting problems with the data processor.

Menu Cannot be Selected

• The [System Status] screen is open.
• Press the [System Status] key to close the [System Status] screen. Select the screen
again.
• The selected screen does not comply with the password security level (the screen names
appear in half-tone color).
• If a half-tone color screen is selected, the temporary log-in window will be
displayed. Contact your supervisor to have the password level changed.
• System Program Crashes
1. Confirm the hard drive LED light is not on.
2. Press the control, alt, delete keys simultaneously.
3. When the window appears select "Shutdown.
4. Turn the power off.
5. Restart the system.
6. Contact Beckman Coulter Technical Services.
• The index of the sample data file to be processed is damaged.
• Select [Maintenance], [Retrieve Data Base]. If the problem is not corrected after
executing [Retrieve Data Base], contact Beckman Coulter Technical Services.

See Also
For information about executing [Retrieve Data Base], refer to the Software Chapter.

Numeric Entry cannot be Performed from the Ten-Key Keypad

• The Num Lock is released.
• Press the Num Lock key, then confirm that the Num Lock LED on the keyboard is
lit.

Disabled Keyboard Access

• Keyboard does not respond
• Check the keyboard cable.
• System Failure
1. Confirm the hard drive LED light is not on.
2. Press the control, alt, delete keys simultaneously.
3. When the window appears select "Shutdown.
4. Turn the power off.
5. Restart the system.
6. Contact Beckman Coulter Technical Services.
• Data is being saved
• Wait until the data is saved.
• The keyboard has been affected by electrical noise.
• Plug the keyboard cable in and out 2 or 3 times.

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AU400/AU400e User's Guide Chapter H - Troubleshooting H-25
April 24, 2004
Inaccessible Floppy Disk
• The floppy disk was formatted differently from the specification.
• Use either of the following floppy disks:
DOS formatted, 2HD 1.44MB
DOS formatted, 2DD 720KB
Format the floppy disk by selecting [Maintenance], [Data Operation], [FD Data
Management]. Select the way you want to initialize the diskette: for parameters or
data. Select function key F5 (start initialize).

See Also
For information about the floppy disk format, refer to the Software Chapter.
• The floppy disk was write-protected when the system attempted to write data to the disk.
Slide the tab on the diskette to “write-enable.”
• The floppy disk is damaged.
• Replace the floppy disk with a new one.
• The floppy disk drive may be damaged.
• If the problem is not corrected after taking the above actions, contact Beckman
Coulter Technical Services.

Analysis Results do not Automatically Print

• Real-time output was not set.
• Set the real-time output of reports or data log lists from the [Printer] screen.

See Also
For information about real-time output setup, refer to the Software Chapter.
• The paper is empty.
• Load more paper. Select [Routine], [Report], then try printing again.

See Also
For information about loading paper, refer to the printer manual supplied with the printer.

See Also
For detailed information about the [Report] screen, refer to the Software Chapter.
On-line Auto-output by Host Computer not Executed
• The I/F cable to the host computer is disconnected.
• Connect the I/F cable correctly.
• The host I/O parameters were incorrectly modified.
• Set the appropriate I/O parameters by selecting the [Online] screen.

See Also
For information about the [Online] screen, refer to [Routine], [Data Report], [Online] located in
the Software Chapter.

No Data Stored Even Though There Is Sufficient Space on the Hard Disk
• The database in the hard disk has been destroyed.
• Select [Maintenance], [Data Operation], [Retrieve Data Base]. Restore the analysis
data that has been backed up on the floppy disk into the hard disk. If the system
will not start up normally after executing [Retrieve Data Base] (the hard disk may
be damaged), contact Beckman Coulter Technical Services.

H-26 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
 See Also
For information about how to rebuild the database, refer to [Maintenance], [Data Operation],
[Retrieve Database] located in the Software Chapter.

Improper Data Input/Output Between the System and Host Computer

• The I/F cable to the host computer is disconnected.
Connect the I/F cable correctly.
• The I/F cable is defective.
Contact Beckman Coulter Technical Services.

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AU400/AU400e User's Guide Chapter H - Troubleshooting H-27
April 24, 2004
2.4 Recovering from an Emergency Stop or Power Loss
In the event of a power failure or an emergency stop, the main power is immediately turned off,
and the power to the incubator and reagent refrigerator is also turned off. Perform the following
procedure to recover from this state of power loss.

Performing an Emergency Stop

Note
The analyzer will stay in a Warm Up mode for 1 1/2 hours after an EM Stop is performed. To bypass
this mode, select [Auxiliary], [Standby Set].

Caution
If the nature of the emergency compromises operator or instrument safety, press the EM Stop Switch
immediately. Please be aware that pressing the EM Stop Switch without performing the other steps
listed below could cause damage to the computer hard drive. Also the operating system and application
files could become corrupted.

The following steps are the preferred method of shutting down during an emergency:
1. Press the following keys on the keyboard: CTRL + ALT + Delete. The Task Manager
appears.
2. From the Task Manager screen, select the Shutdown button. Windows NT will close all
open files and databases.
3. When the message "It is now safe to turn off your computer" appears, press the EM Stop
Switch on the front of the analyzer. All power to the system turns off.

Important
In the event of a stop or an emergency stop, it is not possible to use the data. Analysis must be repeated.
If analysis was in progress and a stop or an emergency stop was performed, reagent still remains in the
cuvettes. This can cause damage or deterioration in the cuvettes, or may cause abnormal results. If
reagent was in the cuvettes for a lengthy period of time, perform a W1 prior to restarting analysis.

Resetting the System after a power failure or an Emergency Stop

1. Press the Reset Switch on the front of the analyzer.
2. After 10 seconds, press the On Switch. The software will load. Wait approximately 20
minutes until the photometer lamp has stabilized before starting analysis. A message
displays for a few minutes "Program download to analyzer." Then an alarm occurs
"Power Failure Detected." Clear the alarm by pressing the "Alarm Clear" button on
screen and Reset.
3. From the [Start Condition] screen, use the same index as before the emergency stop.
Press function key F4 (set). Tab to the "start number" field. Set the "start number" to the
next sample number after any completed data (last sample that printed results).
4. Perform a W1 to remove reagent left in the cuvettes. For detailed procedures on
performing a W1, refer to the Maintenance Chapter.
5. Select [System Status], [Reagent Status]. Reset the "checked" status in the [Reagent
Status] screen. Select function key F5 (Check Start), "Reset Only." Verify reagent
integrity if your instrument was without power for a lengthy period of time.
6. Press start. A warning will appear "ISE No Cal." The ISE will continue to use the last
ISE Cal. data performed, so answer "Yes" at the start prompt.

H-28 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
2.5 Recovering from a Cuvette Wheel Overflow
The following procedure explains what can cause a overflow, and how to recognize and recover
from a overflow. Performing scheduled maintenance greatly reduces the chances of a cuvette
wheel overflow. For more information about maintenance for each system component, refer the
Maintenance Chapter.

2.5.1 What Causes a Overflow?

A wash nozzle is clogged or partially clogged. When this happens, liquid is not aspirated from
the cuvette completely and eventually liquid spills over the side. This can occur when the wash
nozzles are not cleaned properly, or when particles such as glass are aspirated into the nozzle.
• The wash nozzle is bent or damaged.
• Damaged or missing O-rings on tube mounting joint manifolds.
• The reagent probe is bent. A bent probe could be dispensing outside of the cuvette.
• The sample probe is bent. A bent probe could be dispensing outside of the cuvette.
• Cuvettes are chipped or cracked due to alignment problems with the reagent probes or wash
nozzles.
• The wash nozzle tubing is not connected to the nozzle.

2.5.2 Recognizing a Overflow

The error flags *, ?, @, $, D, B, ! may indicate a cuvette wheel overflow. The data, alarms and/
or flags will vary depending on the severity of the overflow. One or all tests could be affected
by a overflow. Here are some problems to look for:
• QC on tests are out-of-range. QC alarms have occurred.
• Reagent blank flags and alarms have occurred on one or more tests.
• Is the entire data printout incorrect?
• The analyzer is not performing as usual.
• Numerous cuvette failures after photocal.
Lift the cuvette wheel cover.
The cuvettes should appear frosty or white, if they are dark, black, or wet when removed, the
cuvette wheel has overflowed.

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November1, 2009
2.5.3 Recovering from a Overflow
Instruction
Immediate attention should be given to a overflow. If nothing is done to fix the problem, the wheel will
continue to overflow.

Check the following list.

• The wash nozzle station should be aligned over the cuvettes. Visually inspect and ensure
nozzles are centered over cuvettes then check the alignment.
• The wash nozzles should be straight. Sonicate and clean the nozzles with a stylus to
remove any debris.
• Check reagent and sample probes to be sure they are properly aligned. Rotate the sample
and reagent probes over the cuvette wheel. If you require assistance performing these
procedures, please contact Beckman Coulter Technical Services.

Instruction
It is very important that the probes are centered over the cuvettes.

• Check for chipped or cracked cuvettes. Replace them if necessary.

See Also
For information about replacing cuvettes, refer to the Maintenance Chapter.
• Verify the wash nozzle tubing connections are secure.
• Verify the O-rings on the manifolds are in place and not damaged.

2.5.4 After the Overflow Problem Is Fixed

Note:
Do not start cleaning cuvettes unless the cause of the overflow is determined.

Please refer to “Cleaning Cuvettes and the Cuvette Wheel after a Cuvette Wheel Overflow”
located in the As Needed Section of this maintenance chapter.

H-30 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August 29, 2008
3. Troubleshooting the ISE
This section provides troubleshooting information for the ISE unit. Performing scheduled
maintenance greatly reduces the chances of problems occurring with the ISE unit. For more
information about maintenance for each system component, refer to the Maintenance Chapter.

Contents

To Begin Troubleshooting the ISE, Answer the Following Questions.....................................H-32

3.1 ISE Sample Requirements...................................................................................................H-34
3.2 Dispensing System................................................................................................................H-35
3.2.1 Sample Probe and ISE Reagent Syringe..................................................................H-36
3.2.2 Pump Tubing:..............................................................................................................H-37
3.2.3 Mix Bar........................................................................................................................H-38
3.2.4 Sample Pot.................................................................................................................H-39
3.2.5 Pinch Valve Tubing.....................................................................................................H-40
3.3 Measuring Components........................................................................................................H-41
3.3.1 O-rings........................................................................................................................H-42
3.3.2 Electrodes...................................................................................................................H-43
3.3.3 Mixture Pump Tubing..................................................................................................H-45
3.3.4 Thermistor...................................................................................................................H-46
3.3.5 Flowcell Block.............................................................................................................H-47
3.4 Calibration Errors...................................................................................................................H-48
3.5 Selectivity Check....................................................................................................................H-50
3.6 Sequential Sample Measure................................................................................................H-51
3.6.1 Shifts & Trends...........................................................................................................H-52
3.6.2 How to Check Reagent Integrity.................................................................................H-54

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-31
April 24, 2004
To Begin Troubleshooting the ISE, Answer the Following Questions.
For data problems
Did you interpret the data printout correctly?

See Also
For information about checking results, refer to “Checking Results” located in the Basic
Operations Chapter.
Is the Data Flagged?

See Also
For information about data flags, refer to the Troubleshooting Chapter.
Is the Calibration Out-of-Range?

See Also
For information about how to perform a calibration, refer to the Basic Operations Chapter.
Is QC Out-of-Range?

See Also
For information about how to perform a QC, refer to the Basic Operations Chapter.
Is Data Erratic? (Have you performed scheduled maintenance?)

See Also
For information about schedule maintenance, refer to the Maintenance Chapter.

For Alarms
An alarm usually indicates a mechanical problem or a communication failure. Follow the
corrective action provided in the on-line help function.

See Also
For information about using on-line help, refer to the Troubleshooting Chapter.

For an Analyzer Mechanical Error

Usually a mechanical error is associated with an alarm. Follow the corrective action provided in
the on-line help function.

For Other Operational Integrity Problems

Were Start-up Procedures Performed Correctly?

See Also
For information on start-up procedures, refer to the Basic Operations Chapter.

Is the Reagent Stable?

See Also
For information on checking reagent stability, refer to the "Troubleshooting for Data Flags"
procedure in the Error Flags Chapter.

Is There Enough Reagent in the Bottles?

An alarm will be generated when ISE reagent is low. Replenish the reagent and make sure it is
in the correct position. Refer to the ISE Maintenance section of the Maintenance Chapter.

Have the Reagent, Calibrator, and QC Material Been Properly Stored?

H-32 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
 See Also
For information on proper storage, refer to the package insert. The "Troubleshooting for Data
Flags" procedure in the Error Flags Chapter also provides general information on handling these
materials.

Has the Analyzer Been Calibrated Correctly?

See Also
For information about performing calibrations, refer to the Basic Operations Chapter.

Have Scheduled Maintenance and Routine Component Replacement Been Performed?

See Also
For information about scheduled maintenance, refer to the Maintenance Chapter.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-33
April 24, 2004
3.1 ISE Sample Requirements
• The ISE is designed to analyze urine and/or serum samples. If problems are encountered
when analyzing a specific test or when using a specific reagent, consult the package insert or
reagent manufacturer or distributor.
• Use urine and/or serum that is free from suspended matter or the probe may be clogged and
adverse affects on analysis may result.
• Exercise care when mixing chemicals (medicine, anticoagulant, preservative, etc.).
• If any suspended matter is recognized in the urine and/or serum to be dispensed, perform
centrifugal separation to precipitate the suspended matter before testing the urine specimen.
• A minimum quantity of sample is required for analysis. Set up an appropriate quantity of
sample for correct sampling in the system, according to this manual.
• To prevent samples from evaporation, do not leave them unsealed for an extended period of
time. If samples evaporate, correct analysis cannot be obtained.
The following list describes some of the sample criteria that can affect data:
1. Properly store and tightly cap samples if analysis is delayed.
2. Follow package insert instructions.
3. High results may occur due to evaporation of the sample.
4. Halogens (bromide and iodine) may affect Cl levels.
5. A hematocrit greater than 65% may affect K levels.

Instruction
See the package insert provided by the reagent manufacturer for a list of acceptable anticoagulants.

See Also
For more information on sample requirements refer to the following: "NCCLS document
(GP16-A), Urinalysis and Collection, Transportation, and Preservation of Urine Specimens:
Approved Guideline," and "College of American Pathologists document, Patient Preparation &
Specimen Handling, Chemistry/Clinical Microscopy (Fascicle VI)."

Other Recommendations for Accurate Data

• Use the recommended control serum
• Keep room temperature constant. Fluctuations can cause data problems.
• Verify all parameter settings.
• Perform scheduled maintenance.

H-34 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
3.2 Dispensing System
If the test results are either inaccurate or imprecise, there could be a problem with the dispensing
system. The following elements make up the dispensing system:
3.2.1 Sample Probe & Syringe (Also used in photometric tests)
3.2.2 Pump Tubing
3.2.3 Mix Bar
3.2.4 Sample Pot
3.2.5 Pinch Valve Tubing
See Also
For information about cleaning or replacement procedures, refer to the Maintenance Chapter.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-35
April 24, 2004
 OLP2061E
3.2.1 Sample Probe and ISE Reagent Syringe
The following components are used when performing ISE tests. Inaccuracy or imprecision could
be caused by the problems listed below (enzymatic tests are particularly susceptible).

ISE reagent syringe

Sample syringe
Reagent syringe Mounting groove

Fixing nut
Fixing screw

FR
ON
T

Case head
Syringe case
Piston fixing screw

Problem: Worn ISE reagent syringe.

Action: Replace the syringe.
Problem: Water carry-over due to a dirty probe.
Action: Clean the probe with an alcohol prep.
Problem: Obstruction in the probe.
Action: Sonicate the probe.
Problem: Misalignment of the probe over the sample pot.
Action: 1. Confirm that the pot has been placed properly.
2. Is the probe tip bent?
3. Contact Beckman Coulter Technical Services.
See Also
For more information on troubleshooting probes, refer to "Probe Problems" in the
Troubleshooting Mechanical Problems section of this chapter.
• General Syringe Troubleshooting.
• Verify the top and bottom screws are hand tight.
• Verify the bottom screw is tight up against the piston.
• Verify there is a smooth, resistant pull.
• Verify the correct size syringe is in use (reagent or sample).
• Verify there is one O-ring being used, and that it is not damaged.
• Verify the syringe is on the analyzer correctly.
• Check the syringe tubing for crimps or leaks.
• Check the teflon syringe tip for wear.

H-36 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
3.2.2 Pump Tubing:

MID solution roller tube

Waste liquid roller tube

T
ON
FR Rolling pump

S
TA
T
E
N
S

D
TA
T

S
E
T
IS
E
P
R
IM
E
OLP2070E

Sample pot

Connector Sample pot

fixing knob

Connector

Mixing unit on the

mount plate

FR
ON
T

Problem: Worn or bent mixture aspiration tubing. This condition could also generate
a sample pot overflow error.
Action: Observe sample pot, flowcell tubing, and bypass tubing during aspiration of
calibrator or control. There must not be any air in the flowcell during the measurement
period. Air will be present in the bypass tubing. If leaks occur or the tubing is worn
or flat in appearance, replace the mixture pump tubing.

See Also
For procedures on replacing mixture pump tubing, refer to the Maintenance Chapter.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-37
April 24, 2004
 OLP2067E

3.2.3 Mix Bar

Mixing unit Knob

Connector
#142

Connector
#143

FR
ON
T

Problem: Mix bar not rotating

Action: 1. Check power connector number 142 to the left of the mix motor.
2. Check that the mix bar is not striking the side of the sample pot. If it is, check
the screws holding the motor in place.
3. Make sure the sample pot is on correctly. If it is on backwards, the mix bar can't
rotate.
4. Contact Beckman Coulter Technical Services.

H-38 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
3.2.4 Sample Pot OLP2070E

Sample pot

Connector Sample pot

fixing knob

Connector

Mixing unit on the

mount plate

FR
ON
T

Problem: A dirty sample pot can cause calibration or QC errors.

Action:
1. Wash the sample pot daily. Refer to the ISE Maintenance section of the Maintenance
Chapter for procedures.
2. Check the tubing and T connectors between the sample pot and electrode. If it is dirty
wash it. For information on washing the sample pot, refer to the ISE Maintenance
section of the Maintenance Chapter. OLP2124
Sample pot

T connector

Electrode unit Tubes

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-39
April 24, 2004
3.2.5 Pinch Valve Tubing

Pinch valve tubing

Pinch valve
4

T
ON
FR

Problem: Calibration errors or sample pot overflow errors.

Action: Align the pinch valve tubing again so that a different part of the tube is in the
valve. If the tube shows any signs of wear, replace the tube. Make sure the
tubing is attached correctly. For information about tubing replacement, refer to
the Maintenance Chapter.

E
S
TA
T
IS
E
P
R
IM

H-40 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
3.3 Measuring Components
The following elements make up the measuring components:
• O-rings
• Electrodes
• Mixture Pump Tubing
• Thermistor
• Flowcell Block

To Determine if There Is a Flowcell Problem

1. Evaluate the calibration data.
2. Perform a Sequential Sample Measure in the [Diagnostics] screen. For more information,
refer to "Sequential Sample Measure," located in the ISE Troubleshooting section of this
chapter.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-41
April 24, 2004
3.3.1 O-rings
Problem: Missing O-ring
Action: Verify that there are a total of four (4) O-rings: cell inlet block, Cl electrode, Na
electrode, and K electrode.
Problem: Defective O-ring
Action: The O-rings should be positioned correctly. Make sure they are not flat, bent or
misshapen. Replace them if necessary.
Problem: O-ring contaminated with KCl
Action: Remove the O-ring and wash it along with the groove in deionized water to
remove any residual KCl. KCl will contaminate the electrodes and affect results.

H-42 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
3.3.2 Electrodes OLP2074

Lock lever (The electrodes

are locked in this state.)

K electrode
Na electrode
Cl electrode

Cord

STAT ROTATION/DIAG switch

S
TA
T
E
N
S

D
TA
T

S
E
T
IS
E
P
R
IM
E
Problem: Old or obstructed Na, K, Cl, or reference electrodes
Action:
1. Remove obstructions in the flowcell path. Perform the daily cleaning procedure two or
three times. Prime with mid-standard.
2. Perform a calibration.
See Also
For information about performing calibrations, refer to the Basic Operations Chapter.
3. Perform a “Selectivity Check” to verify Na and K membrane selectivity.
See Also
OLP2077
For information about performing a selectivity check, refer to the Troubleshooting Chapter.
4. Try changing electrodes.
See Also
For information about replacing electrodes, refer to the "ISE Maintenance" section of the Maintenance
Chapter.

Connector

REF electrode
insertion hole
Packing
Set screw
REF electrode Block

T
ON
FR

Cross-sectional View of REF Electrode

This end up
Electrode bar

REF electrode

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-43
April 24, 2004
 Problem: Bubble in the reference electrode
Action: Check for a bubble in the reference electrode by removing and visually inspecting
the electrode tip. Gently tap the tip to dislodge the bubble, then replace the
electrode.
Problem: Reference electrode not installed properly
Action: Install the electrode properly by referring to the replacement procedure in the ISE
Maintenance section of the Maintenance Chapter.
Problem: Broken lead wires connecting to the electrodes
Action: Call Beckman Coulter Technical Services.
Problem: Missing reference electrode packing and internal solution
Action: Reference electrode packing and internal solution can be replaced if necessary.

See Also
For information about adding reference electrode solution, refer to the Maintenance Chapter.

Instruction
Electrode replacement should be performed if the slope values are out-of-range, or if other
troubleshooting measures don’t resolve the problem. If possible, remove the suspected electrode and
place it into another AU400 and perform a calibration to verify the performance of the electrode.

H-44 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
 OLP2071E

3.3.3 Mixture Pump Tubing

MID solution roller tube

Waste liquid roller tube

T
ON
FR Rolling pump

S
TA
T
E
N
S

D
TA
T

S
E
T
IS
E
P
R
IM
Problem: Old, worn, or crimped tubing or leaking

E
Instruction
Old or worn tubing will be flat (no longer round in diameter) in the area where it is pressed
against the pump.

Action:
1. The mixture pump pulls the samples, calibrators, and mid-standard through the flowcell.
Replace the mixture pump tubing.
See Also
For information about replacing mixture pump tubing, refer to the "ISE Maintenance" section of the
Maintenance Chapter.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-45
April 24, 2004
 OLP2076E

3.3.4 Thermistor
Lock lever
(Electrodes are locked in this state.)
Thermistor connector

Thermistor

REF electrode cord

T
ON
FR

If the following problems occur with the thermistor, perform the action listed below:
Problem:
• Defective packing

S
TA
T
E
N
S

D
TA
• No contact with liquid in the flowcell

S
E
T
IS
E
P
R
IM
• Other thermistor malfunction

E
Action:
1. Visually check the thermistor for liquid contact. Verify the thermistor was not forced in,
and that the screw is tightened. No bubbles should be observed around the thermistor.
2. Call Beckman Coulter Technical Services if a malfunction of the thermistor is suspected.

H-46 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
3.3.5 Flowcell Block OLP2074

Lock lever (The electrodes

are locked in this state.)

K electrode
Na electrode
Cl electrode

Cord

STAT ROTATION/DIAG switch

S
TA
T
E
N
S

D
TA
T

S
E
T
IS
E
P
R
IM
E
If the following problems occur with the flowcell block, perform the action sequence stated
below:
Problem:
• Obstruction in the flowcell inlet block
• Obstruction in the flowcell outlet block
• Defective port of flowcell inlet block
•
Action:
1. Check the expiration date of the electrodes. The expiration date is located on the box. If
the electrodes have expired, do not use them.
2. Replace defective flowcell inlet or outlet blocks.
3. Perform an ISE cleaning procedure. Call Beckman Coulter Technical Services if the
obstruction is not removed.

Instruction
To determine electrode integrity and on-board life, review the calibration, selectivity check, and QC
results. The following calibration ranges are acceptable:

Na 38 - 65
K 38 - 65
Cl -38 - -65
Mid-standard solution factor ranges:

Na 0.80-1.20
K 0.75-1.25
Cl 0.75-1.25
Replace electrodes when results are out-of-range and other troubleshooting procedures have been
performed. Electrodes are under warranty for 20,000 samples or 6 months.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-47
April 24, 2004
3.4 Calibration Errors
Calibrate the ISE daily. If the standards were in the standard cup table for more than fifteen (15)
minutes, replace with fresh standards.

See Also
For information about performing calibrations, refer to the Basic Operations Chapter. To perform a
calibration from diagnostics, refer to the Software Chapter.
1. Verify calibration results.
2. Review the data daily by using the following criteria:
A. The slopes must be in range:
Na 38 - 65
K 38 - 65
Cl -38 - -65
Mid-standard solution factor ranges:

Na 0.80-1.20
K 0.75-1.25
Cl 0.75-1.25

B. The day-to-day slope values should be consistent for each electrode. Slopes may
decrease with time. This however, does not necessarily indicate a faulty electrode.
C. Check for trends in slope values.
3. Verify the ISE Reagent Integrity.

Instruction
A calibration failure will occur if the buffer or standard solutions become contaminated with the
reference solution. The reference solution has a very high concentration of KCl and even a small
amount will affect ISE results.

4. Verify the Dispensing System.

5. Verify Measuring Components.
6. Perform a calibration to obtain the electrical potential of the low, mid, and high standards.
7. If the Na and K slopes are negative and the Cl slope is positive:
High Standard vs. Low Standard: A slope will be generated if the standards are
reversed on the standard cup table. If this happens, the Na and K slope values will be
negative and the Cl slope value will be positive. Pour the standards again, and place
them in the correct positions on the standard cup table.

H-48 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
 8. The Na and Cl are out-of-range:
A possible carry-over from the K selectivity solution. Perform a calibration three (3)
times and verify results.
9. The Na and K are out-of-range:
A possible cause is contamination in the sample pot. To resolve this problem, sonicate
the sample pot.
10. Na/K and Cl have a shift in the opposite direction. Check the reference electrode.
See Also
For information about replacing the reference electrode, refer to the Maintenance Chapter.
11. Na/K and Cl have a shift in the same direction:
• Verify the integrity of the buffer reagent.
• Visually inspect the thermistor.

See Also
For information about replacing reagents, refer to the Maintenance Chapter.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-49
April 24, 2004
3.5 Selectivity Check
Perform a Selectivity Check for the Na/K Electrodes
The Na electrode and K electrode are ion-selective electrodes. If the selectivity of electrodes
deteriorates, the ISE unit is affected by ions other than those being measured. Appropriate
analysis results will not be obtained. To check the electrodes for deterioration, check the
selectivity of the Na and K electrodes every week.

Instruction
A successful calibration must be achieved before performing a selectivity check.

See Also
For information about performing a selectivity check and replacing electrodes, refer to the "ISE
Maintenance" section in the Maintenance Chapter.

H-50 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
3.6 Sequential Sample Measure
The sequential sample measure checks ISE precision. For detailed procedures, refer to
[Maintenance], [Maker Maintenance], [ISE Diagnostics] in the Software Chapter. Perform a
Sequence 1, 2, and 3.
1. If a value is out-of-range after performing a check, try the following:
If only one value is out-of-range:
• Check the corresponding electrode lead or replace the electrode.

See Also
For information about replacing the electrodes, refer to the Maintenance Chapter.
If all three values are out-of-range in the same direction:
• See “3.2 Dispensing Systems”
• See “3.3 Measuring Components”
• Replace the sample probe
If all three values are randomly out-of-range:
• See “3.3 Measuring Components”
• Check all electrodes
• Check reference electrode packing and internal solution

See Also
For information about replacing the solution, refer to the Maintenance Chapter.
2. Repeat the sequential sample measure until SDs are in the acceptable range.
3. Out-of-Range QC results are flagged with H or L on the printout. Verify that the range
for the QC lot number is properly entered in QC parameters.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-51
April 24, 2004
3.6.1 Shifts & Trends
Problem:
• A SHIFT is more than 7-10 QC points in a row that fall either above or below the
mean.
• A TREND is 5-10 QC points in a row that are steadily increasing or decreasing.
1. Each laboratory must determine the number of QC points that define a shift or trend.
2. Shifts or trends will be flagged by the AU400 QC program.
3. Check for shifts or trends by reviewing the Daily QC Chart Output Screen
4. Preset or cumulative modes are available. The laboratory must decide what to use.
A. Preset values are determined from the package insert.
B. Cumulative values are calculated by indicating the start date and end date of the QC
files the laboratory wants included in the calculation.
Action:
1. Verify the QC material:
A. Check the lot number and expiration date.
B. Check the stability for storage conditions on the QC material (freezer, refrigerator,
opened bottles).
C. If using lyophilized QC, check the reconstituted stability and verify that it was made
correctly. Use a volumetric pipet to add the DI water.
D. Verify the QC material was not left at room temperature for an extended time period.
Avoid prolonged exposure to air before processing, because evaporation will affect
analyte concentration.
E. Verify that QC material was not contaminated prior to, or during the run. Check
for indications of instability such as abnormal color, turbidity, or a precipitate. Use
fresh material if necessary. Refer to the QC package insert for specific indications of
deterioration.
F. Verify that the QC material is mixed well.
2. If using an external calibrator (ACAL) for the electrolytes, verify the calibrator material
using the same criteria as above.
3. Place the control samples in the green rack in the order of the control numbers which
were set as parameters. The control numbers correspond to the sample positions (1 to 20)
in the green rack. For example, 1 to 10 are the control #s. and sample positions for the
green rack of ID 1, and 11 to 20 are the control #s. and sample positions for the green
rack of rack ID 2.
4. Verify the dispensing system. (See 3.2 Dispensing Systems)
5. Verify all reagent integrity.

H-52 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
 See Also
For information about on board stability and reagent replacement, refer to the Maintenance Chapter.
6. Verify the integrity of the measuring components. (See 3.3 Measuring Components)
7. Verify the software parameters.

Instruction
Check the high and low range against the package insert values. Verify that the lot number in use
corresponds to these values. This range generates H and L flags on the printout.

8. If using an external calibrator (ACAL), verify that the correct calibrator concentration is
entered.
9. Verify that the correct Cal No. is being used.
10. Select [Parameters], [Specific Test Parameters]. Check the factor and offset values. The
factor should be 1.0 and the offset 0.0 unless results are being corrected.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter H - Troubleshooting H-53
April 24, 2004
3.6.2 How to Check Reagent Integrity
1. Check the lot # and expiration date. Check the stability for storage conditions on the
reagent. Follow the directions on the package insert.
See Also
For information about on board stability and reagent replacement, refer to the Maintenance Chapter.
2. Verify the reagent material was not contaminated at any point prior to or during the run.
3. Check for indications of instability such as abnormal color, turbidity, or a precipitate.
Use fresh material if necessary. Refer to the package insert for preparation instructions
and specific indications of deterioration.
4. Verify that the reagent is placed in the compartment properly and that the correct
parameters were entered. Refer to the Basic Operations Chapter for procedures on
placing reagents.
5. Do not combine old reagent with fresh reagent. Always date new bottles before placing
them on the unit.
6. Prime lines with fresh reagent.

Instruction
Prime the reference solution approximately five (5) times to completely fill the lines.

H-54 Chapter H - Troubleshooting

Automated Chemistry Analyzer
AU400/AU400e User's Guide
April 24, 2004
 Chapter I
Specifications

Introduction
This Chapter provides an outline of system operation during analysis and hardware configuration
descriptions of the analyzer. It shows the piping diagram and major specifications of this
system. Also, terminology specific to the AU400 is described here.

Contents
1. System Operation During Analysis................................................................... I-3
Operation Process..................................................................................................................... I-3
Operation Modes....................................................................................................................... I-4
2. Analyzer Hardware Configuration..................................................................... I-5
Operation Switches................................................................................................................... I-5
Rack Feeder Unit....................................................................................................................... I-7
Sample Probe and Reagent Probe Units.................................................................................. I-9
The Mix Unit............................................................................................................................. I-10
The Wash Nozzle Unit..............................................................................................................I-11
The STAT Table....................................................................................................................... I-12
Sample Syringe and Reagent Syringes................................................................................... I-14
Rolling Pump Unit.................................................................................................................... I-15
Reagent Refrigeration Unit...................................................................................................... I-16
The Incubator.......................................................................................................................... I-18
The Photometer Unit............................................................................................................... I-19
Tank Storage........................................................................................................................... I-20
Breakers and Fuse.................................................................................................................. I-21
The ISE Unit ........................................................................................................................... I-22
The ISE Reagent Syringe ....................................................................................................... I-23
The ISE Reagent Bottles......................................................................................................... I-24
3. System Connections......................................................................................... I-25

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-1
August, 2002
 4. Major System Specifications............................................................................ I-26
Specifications Related to System Installation.......................................................................... I-26
Sampling Specifications.......................................................................................................... I-27
Reaction Unit Specifications.................................................................................................... I-28
Photometer Unit Specifications............................................................................................... I-29
Data Processor Unit Specifications......................................................................................... I-29
Analysis Processing Specifications......................................................................................... I-29
Input/Output Specifications...................................................................................................... I-30
ISE Unit Specifications............................................................................................................ I-34
5. Calculations....................................................................................................... I-35
5.1 Reagent Blank (Zero Adjustment)........................................................................................... I-35
5.2 Endpoint Assay........................................................................................................................ I-37
5.3 Rate Assay............................................................................................................................... I-39
5.4 Fixed Point Assay.................................................................................................................... I-40
5.5 Sample Blank........................................................................................................................... I-41
5.6 LIH........................................................................................................................................... I-42
6. Quality Control.................................................................................................. I-43
7. AU400 Terminology........................................................................................... I-49

I-2 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
1. System Operation During Analysis
This section describes the system operation and operation modes during analysis.
OLP4065E
Operation Process

Cuvette wheel
Mixing
Washing and drying
Reagent transfer
Grating

Photometer sensor
Light
source Auto repeat run

Sample transfer

Reagent refrigeration unit

STAT table
Rack feeder

ISE unit
Sample transfer
Mixing bar
Color CRT Standard
Report CPU
monitor printer

Sample pot
Optional
Report printer

Electrode
Hard
Record and data storage disk

Keyboard

Rolling Pump Rolling

Pump

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-3
August, 2002
Operation Modes OLP4066E

Power ON Initialize

Maintenance Warm-up

W1 W2 Photocal measurement

System shutdown Standby

Power OFF

Measure 1 Measure 1 :
Sample analysis in progess.
Pause
Measure 2 :
No more racks detected on the feeder belt.

Measure 3 :
Measure 2 All R1 dispensing is completed.

Pause Measure 4 :
Final Curvette wash.

Measure 5 :
(Not displayed) When a nonfatal mechanical error
occurs during analysis with one of more samples
Measure 3 having the R2 mixed prior to the nonfatal
Pause mechanical error Processing will finish for the
samples that have R2 mixed.

Measure 1, Measure 2, and

Measure 3 can be restarted.

Measure 4
Pause

Measure 5 Stop

: Automatic transition
: User operated transition

Measure 1: Sample analysis is in progress. Racks are moving from the feeder belt to the

transfer belt.

Measure 2: No more racks are detected on the feeder belt.

Measure 3: All R1 dispensing is completed.

Measure End (4): Final cuvette wash.

Measure 5: (Not displayed) When a nonfatal mechanical error occurs during analysis with

one or more samples having R2 mixed prior to the nonfatal mechanical error.

Processing will finish for the samples that have R2 mixed.

One Touch Mode: Preprogrammed STAT analysis mode. Sample requisition is not required.

See Also
For more information on the One Touch Mode, refer to the Basic Operations Chapter.

I-4 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
2. AnalyzerOLP2086E
Hardware Configuration
This section describes the hardware configuration of each part of the AU400 analyzer.

Operation Switches

STAT Test STAT LED (END LED)

Button STAT LED (SET LED)

ST
AT
STAT ROTATION/DIAG
Button

Power-ON Button (Sub-power button)

EM STOP Button Main breaker
RESET Button (main power
supply)

ISE
ON

F
OF
ISE Power Switch

FR
ON
T

Power-on Button (Sub-power switch)

This button loads the software, and the lamp turns on.
STAT LED (Set LED, End LED)
SET LED: The SET LED indicates when the STAT table is ready to receive sample cups. If
the light is on, it is possible to place sample cups on the table. If the light is off, sample cup
placement is not possible.
END LED: The END LED indicates when the system has completed aspiration of the
samples on the STAT table. If the light is on, aspiration is complete. If the light is off,
aspiration is not complete.
STAT Test Button
This button starts analysis on the STAT table.
STAT ROTATION/DIAG Button
This rotates the STAT table. The STAT table rotates 1/3 of a complete rotation each time the
button is pressed. Also, press this button after selecting an operational item to be performed
using the [ANL Maintenance] screen or the [Diagnostics] screen. The function will begin.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-5
August, 2002
 See Also
For detailed information about the [ANL Maintenance] screen, refer to the Software Chapter.
This button also executes the Prime operation for discharging or supplying solution when
performing ISE prime functions. Select [System Status], [ISE Status] the prime operation,
and press the STAT ROTATION/DIAG button.
See Also
For detailed information about the ISE Status function, refer to the Software Chapter.
EM STOP (Emergency Stop) Button
Use this button to perform an emergency stop. In the event of a power failure, or an
emergency stop, the main power is immediately turned off, including the power to the
incubator and reagent refrigerator. To recover from a power loss, perform the "Recovering
from an Emergency Stop or Power Loss" procedure located in the Troubleshooting Chapter.
RESET (Main Power) Button
This button is used to turn on the main power after the system was stopped using the EM
STOP Button. When the RESET Button is pressed, the main power (including power to the
incubator and reagent refrigeration unit) turns on. The system is equipped with a power-
failure detection circuit. After power failure is detected, the circuit must be reset by pressing
the RESET Button. A message displays to inform the operator of the power failure.
ISE Power Switch
This switch is used for turning on/off power to the ISE. When the system power is on, it is
possible to turn off power to the ISE without turning off power to any other unit. The ISE
Power Switch must be on if ISE analysis is to be performed.

I-6 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
Rack Feeder Unit OLP2087E

Sample/Rack
barcode reader
Reflecting mirror

Rack feeder
plates
Protective
cover
LED
Rack
T
ON
FR

Top view of the LED

Sample Protective Cover LED

DO NOT SET A RACK WHEN BLINKING

Prevents dust from entering samples. It also prevents the sample from evaporating. The
cover should always be closed during analysis.
Rack
Samples are placed on the rack. It is possible to set a maximum of 10 samples on one rack.
The rack color differs depending on the type of analysis.
Rack Feeder Plates
Plates move the rack. The racks should be placed between the rack feed plates.
Sample Barcode Reader
Reads the sample ID on tubes.

Caution
Do not look directly at the laser beam emitted from the sample/rack barcode reader. Staring at the beam
may damage your eyes.

Reflecting Mirror
Reflects the sample barcode reader laser beam onto the sample tube.

Instruction
Do not touch the mirror with your fingers or any hard materials. If the mirror is smudged or scratched,
barcode reading may not be performed properly.

Rack Barcode Reader

Reads barcodes on the rack.
Window for Rack Barcode Reading
Where the laser comes out to read the rack ID.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-7
August, 2002
 Rack LED
The LED lights immediately before the rack feed plate starts moving and flashes continuously
while the rack feed plate is moving. Do not set a new rack on the unit while the LED is
flashing, this could cause spills on the rack unit.

I-8 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
 OLP2088E

Sample Probe and Reagent Probe Units

Probe arm
Sample probe cover

Reagent probe

Relay tube
Probe connector

Sample probe
Reagent probe
wash well
Sample probe
wash well
T
ON
FR
Probe arm

Sample probe
Dispenses a given volume of sample in a cuvette.
Reagent probe
Dispenses a given volume of reagent in a cuvette.
Probe connector
Connects the sample probe and reagent probe to the relay tube.
Probe arm
Supports the sample probe and reagent probe.
Sample probe wash well
Washes the sample probe.
Reagent probe wash well
Washes the reagent probe.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-9
August, 2002
 OLP2089E

The Mix Unit

Mix unit
Mix bar

Mix bar
wash well

T
ON
FR

Mix Unit
This unit controls the mix bars. The sample and reagent inside a cuvette are mixed by
a rotating mix bar. The mix unit also rotates the bars to the wash well. The mix unit is
comprised of one mixer. There are six mix bars in the mixer.
Mix Bar
After the sample and reagent have been dispensed into a cuvette, the mix bar rotates and
mixes the mixture in the cuvette.
Mix Bar Wash Well
This is used for washing the mix bar after mixing.

I-10 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
 OLP2090E

The Wash Nozzle Unit

Tube mounting joints

Knob
Joint tube
Tube mounting joints

Wash nozzles
Wash nozzle
station FR
Aspiration nozzle ON
T
Drying nozzle

Wash nozzles

Wash Nozzles
Used for washing the cuvette after analysis is complete. Each nozzle is made up of three
small nozzles. The longest nozzle aspirates the reaction mixture, detergent, and wash water.
The next longest nozzle dispenses the detergent or wash water. The shortest nozzle aspirates
any detergent or wash water that exceeds a predetermined amount.
Aspiration nozzle
Aspirates the dispensed wash water.
Drying nozzle
Aspirates the remaining drops of water inside the cuvette.
Tube Mounting Joints
Connects each of the nozzles to the internal drain tubing.
Knob
Secures the wash nozzle station.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-11
August, 2002
 OLP2091E

The STAT Table

STAT table
cover (small)

Sample cup for the

STAT table

STAT table STAT table

21
22
1

U-L
2
3

4
cover (large)
U-H

5
20

S-L
K-S

6
EL-
Na
19

7
S-H
18

8
17
W1

9
CLEA

16
N
W2 10
15

T
11
14

ON
13 12

S
FR

TA
T
E
N
S

D
TA
T

S
E
T
IS
E
P
R
IM
E
Top view of STAT table

3 4
2
5
1

6
U-L
22

7
U-
H
21

8
S-L
K-SEL-Na
20

9
19

S-H

W1

10
18

2
W

11
CLEAN
17

12
16
13
15 14

STAT table
Used to perform urgent analysis, automatic QC and automatic calibration. There are cup
positions around the inside and outside of the STAT table. The samples to be analyzed
are set in the cup positions around the outside circumference of the table. Detergent or
reagents used for ISE unit calibration or selectivity checks are set in the positions around
the inside circumference of the table. It is possible for the system to read only the barcodes
on the sample cups that are set in the outer positions on the table. Note: The STAT table is
refrigerated, after placing samples, be sure to replace the table covers. Removing the table
covers frequently may cause the temperature on the table to become unstable.
“WASH 1” Position
During normal analysis, the sample cup (containing 2% Washing Solution for washing the
sample probe) is placed in this position.
“WASH 2” Position
The bleach or HCl for washing the sample probe is poured in a sample cup and placed in this
position.
“CLEAN” Position
When automatically washing the ISE sample pot and electrode line, the sample cup containing
the bleach is placed in this position.

I-12 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
January 20, 2006
 “S-H” and “S-L” Positions
Used for sample cups containing calibrator solutions of serum for the ISE unit. ISE high
serum standard is set in the “S-H” position, and ISE low serum standard is set in the “S-
L” position. Also, use these positions to place sample cups containing the solution used
in checking the ISE electrode selectivity. The solution for the Na check is set in the
“S-H”(“SEL-Na”) position, and the solution for the K check is set in the “S-L”(“K-SEL”)
position.
“U-H” and “U-L” Positions
Used for sample cups containing the calibration solution of urine for the ISE unit. ISE high
urine standard is set in the “U-H” position, and ISE low urine standard is set in the “U-L”
position.

Instruction
Do not place barcoded sample cups in the inner positions on the STAT table. Barcode read errors may
occur with the sample cups placed in the inner positions.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-13
August, 2002
 OLP2092E

Sample Syringe and Reagent Syringes

ISE reagent dispenser

Sample dispenser Fixing nut
Reagent dispenser Case head
Relay tube

Syringe case
FR
ON
T
Piston fixing screw

Sample Syringe
Used to supply a small fixed amount of sample.
Reagent Syringe
Used to supply a small fixed amount of reagent.
Fixing Nut
Attaches the syringe to the syringe unit.
Syringe Head
Secures the syringe together with the syringe case.
Syringe Case
This case holds the syringe.
Piston Fixing Screw
Attaches the syringe piston to the syringe drive assembly.

I-14 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
 OLP2109

Rolling Pump Unit

Rolling tubes

Rolling pumps
for detergents

Connectors

Relay tubes
ONT
FR

Rolling Pump
Used to aspirate concentrated detergent and dispense it into the diluted detergent tank. There
are two rolling pumps; one for detergent tank A (factory optional) and one for detergent tank
B.
Rolling Tube
The rubber tube on the rolling pump. Detergent is supplied or drained through the tubing.
There are two rolling tubes; one for detergent tank A (factory optional) and one for detergent
tank B.
Relay Tube
The relay tubes are used to connect the rolling tubes through the connectors as shown in the
illustration above.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-15
August, 2002
Reagent Refrigeration Unit OLP2093E

Reagent
refrigerator cover

Positioning pin

Sample pre-dilution
Reagent refrigerator bottle position
T
ON
FR

Example: Top view of the reagent refrigerator

(The reagent refrigerator cover was removed.)
Reagent bottle
Reagent bottle (60mL)
(30mL) Reagent bottle
Reagent bottle (30mL)
(15mL) Reagent bottle
Reagent tray (15mL)

Partition
Positioning pin
Partition
Partition

Reagent refrigeration unit

Refrigerates the reagent bottles. Both the first and second reagent bottles are set in the same
reagent refrigerator. The refrigerators are kept between 4 and 12 degrees C.
Reagent refrigerator cover
Prevents the reagent from evaporating, and prevents dust from getting into the reagent bottle.
Also, it maintains the temperature of the reagent refrigerator between 4 and 12 degrees C.
Reagent tray
Used to set reagent bottles. A maximum of 76 reagent bottles can be placed in the reagent
compartment.
Reagent bottles
The reagent bottles are containers for holding reagent. Reagent bottles with a capacity of 60
mL, 30 mL or 15 mL can be placed on the reagent tray.
Use the appropriate partition when placing 15 mL reagent bottles on the outer circumference
of the tray, and 30 mL or 15 mL reagent bottles on the inner circumference of the tray.
Always use a partition for the 15 mL reagent bottles.

Caution
Place reagent bottles with the barcode label facing the outside of the reagent tray. If the reagent bottles
are set in any other way, the reagent probe may be damaged.

Sample Pre-dilution Bottle Position

Use this position to place the sample pre-dilution bottle filled with DI water for sample
dilution.

I-16 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
 Caution
When placing the sample pre-dilution bottle on the analyzer, be sure it does not protrude above the top
analyzer surface or the reagent probe may be damaged. Do not put the cap on the bottle when it is on
the analyzer, this causes the reagent probe to crash.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-17
August, 2002
The Incubator

Cuvette wheel cover

Cuvette wheel

Incubator

T
ON
FR

Cuvette

Incubator
The cuvette wheel is attached to the incubator. The mixture in each of the cuvettes in the
cuvette wheel is incubated in the incubator. During analysis, the temperature in the incubator
is kept at 37°C.
Cuvette Wheel
The cuvette wheel contains 88 cuvettes. As the cuvette wheel rotates, a series of analyses are
performed from dispensing of the sample to performing photometry.
Cuvettes
These are square shaped containers made of glass used in analysis. The sample and reagent
are dispensed into the cuvettes. The light path of a cuvette is 6 mm, and the capacity is 750
µl.

I-18 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
The Photometer Unit

Lamp cover

Knobs
Photometer
lamp

T
ON
FR Lamp cords

Photometer Lamp
A white light source used in performing photometry on the mixture in the cuvettes.
Lamp Cover
The cover is for safety since the photometer lamp becomes very hot. Also, it prevents electric
shock due to touching the terminal board of the photometer lamp, and shields the inside of the
photometer unit.
Knobs
The knobs connect the lamp cords to the terminal board. A DC voltage of approximately +12
V is output.
Lamp Cords
The lamp cords supply power to the photometer lamp.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-19
August, 2002
Tank Storage OLP2096E

Deionized water tank

Diluted detergent tank A
(factory optional)
Diluted detergent tank B

Concentrated Concentrated
detergent tank A detergent tank B
(factory optional)

Deionized Water Tank

This tank stores the deionized water. The deionized water is produced from tap water using a
deionizer. The capacity of this tank is 10 liters.
Diluted Detergent Tank A (Factory Optional)
This tank stores 2% detergent that has been diluted from the concentrated detergent A using
deionized water. The capacity of this tank is 2 liters.
Diluted Detergent Tank B
This tank stores 2% detergent that has been diluted from the concentrated detergent B using
deionized water. The capacity of this tank is 2 liters.
Concentrated Detergent Tanks A (Factory Optional) and B
These tanks store concentrated extran detergent. The capacity of each tank is 2 liters. The
recommended detergents are listed below.
Tank Detergent Used Detergent Supplied To
A (factory optional) Extran Cuvette wash unit
B Extran Cuvette wash unit & mix bar unit

I-20 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
Breakers and Fuse OLP2097E

Rear view
DPR (PC) breaker
Reagent refrigerator and
incubator breaker
Analyzer breaker
Fuse Heater breaker

FUSE
Pilot lamp 250V 2A
TYPE-F

P.L. 7.5A 15A 15A 15A

PC REF/ISE ANL HEATER
COM.

DPR (PC)
service outlets OP.1 SIGNAL OP.2 SIGNAL

OP.1 POWER OP.2 POWER OPD

System main breaker AC100V
7.5A

System Main Breaker

This is the main power breaker for the entire system. 208-volt system (USA): 20 A
Analyzer breaker
This breaker is for the power source (15 A) of the analyzer drive unit and control board.
Reagent Refrigerator and Incubator Breaker
This breaker is for the power source (15 A) for the reagent refrigerator, incubator, ISE unit
and some control boards.
DPR (PC) Breaker
This is a 7.5-amp breaker.
Pilot Lamp
Light to indicate that the system is supplied with power.
Fuse
If the pilot lamp does not light even though the main breaker is on, the fuse may be blown.
Only use the specified fuse given in the list of consumables.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-21
August, 2002
The ISE Unit OLP2098E

K electrode
Pinch valve Na electrode
Thermistor Cl electrode

Rolling tubes REF

electrode

Mixing unit

T
ON
FR MID solution dispense rolling pump
Mixture aspiration rolling pump Sample pot

S
TA
T
E
N
S

D
TA
T

S
E
Sample Pot

T
IS
E
P
R
IM
E
The sample and buffer solution are dispensed into the sample pot and mixed. The
amount of dispensed sample is 20 µl of serum (fixed), 25 µl of urine (fixed), and 10
µl of DI water (fixed). The amount of dispensed buffer solution is 618 µl for serum
(fixed) and 750 µl for urine (fixed).
Mixing Unit
This unit mixes sample and buffer solution dispensed into the sample pot. It is
equipped with two liquid-level sensors to detect clogged tubing.
Cl Electrode, Na Electrode and K Electrode
These electrodes are used for measuring the potentials of Cl, Na and K ions in the
mixture and MID solution. The concentrations of individual ions in the mixture can
be calculated from the potential differences between each ion in the mixture and in
the MID solution.
REF Electrode
This is the reference electrode with respect to the Cl, Na and K electrodes.
Thermistor
The potentials of the Cl, Na and K electrodes change depending on the temperature
of solutions even though the concentrations are identical. To remove the effects of
temperature, the output potentials are corrected by the thermistor.
Pinch Valve
This valve is used for alternately discharging the analyzed mixture and the mixture
remaining in the sample pot.
Rolling Pumps
There are two pumps; a rolling pump which aspirates the mixture solution, and a
rolling pump which dispenses the MID solution.
Rolling Tubes
These tubes are made of rubber and wrap around the rolling pump. As the rolling
pump rotates, the tubes are squeezed by the rollers on the pump, and solution is
supplied or removed.

I-22 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
The ISE Reagent Syringe
ISE reagent dispenser Fixing nut
Sample dispenser
Case head
Reagent dispenser
Relay tube

Syringe case

Piston fixing screw

FR
ON
T

Fixing Nut
Attaches the syringe to the analyzer.
Case Head
Attaches the syringe together with the syringe case.
Syringe Case
Holds the syringe.
Piston Fixing Screw
Attaches the syringe piston to the syringe drive assembly.

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-23
August, 2002
The ISE Reagent Bottles

ISE
ON

F
OF

Buffer solution tank

MID solution tank

REF solution tank

FR
ON
T

Buffer Solution Bottle

Stores the buffer solution used for diluting the sample. The capacity of this container is 2 liters.

MID Solution Bottle

Stores the MID solution. The capacity of this container is 2 liters.

REF Solution Bottle

Stores the REF solution. The capacity of this container is 1 liter.

I-24 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
August, 2002
OLP4099

AU400/AU400e User's Guide

Automated Chemistry Analyzer
POWER
INPUT
System Connections Diagram

(OPTION) (OPTION) (OPTION)

WATER
WASTE WASTE
SUPPLY
PUMP PUMP
VALVE
UNIT UNIT PWR
UNIT
CNTR
PRINTER

OP.2 OP.1 OPD

PWR
3. System Connections

SIGNAL POWER SIGNAL POWER POWER PWR COM. POWER

OUTLET
COM1

MOUS
POWER
PRINTER

MONITOR MONITOR
KYB
MONITOR
PERSONAL
COMPUTER
AU400 ANALYZER
MOUSE
KEY BOARD

I-25 Chapter I Specifications

4. Major System Specifications
The specifications for the AU400 system are listed below.

See Also
For more information on installation environments, refer to the "Precautions on Use and Installation
Environments" Chapter. Note: Check the rating plate on the rear face of the unit for the unit type.

Specifications Related to System Installation

Dimensions & Weight

Analyzer: (Inches) Width 57" x Depth 30" x Height 48" Weight 924 lbs
Data processor: Width 27.6" x Depth 27.6" x Height 62" Weight 146 lbs The data processor
(DPR) includes the rack, personal computer, CRT monitor and printer.

Water Supply and Drainage

Water Type Deionized CAP Type II, Bacteria Free

Water pressure 0.49 x 105 to 3.92 x 105 Pa (4-57 psig)
Water consumption: (unit type 403-02) Average: 20 L/hour (50Hz/60 Hz) (unit type 401-02)
Average: 26L/hour (50Hx/60Hz) Maximum: 0.7 L/min.
(50Hz/60Hz)
Water supply hose 30 feet or less
Concentrated waste liquid hose 30 feet or less
Diluted waste liquid hose 30 feet or less
Drainage height 4 inches or less (from the installation floor) If the optional
forced drainage equipment is used, it should be 4 feet or less.
Air exhaust hose 30 feet or less

Operating Environment

Temperature: 18 to 32 °C
Humidity: 40 to 80% RH (with no condensation)
Temperature fluctuation: ±2 °C or less during analysis
Conductivity of deionized water: 2.0 µs/cm or less (0.5 MΩ or greater)
Temperature of deionized water: 5 to 28 °C
Maximum altitude: 6,500 feet (2,000 M)
Pollution degree: 2
Installation category: II

Power Supply

AC210V ±10% (U.S.A) 60Hz

AC230V ±10% (Europe) 50Hz
AC110V ±10% (Asia) 50/60Hz
AC220V ±10% (Asia) 50/60Hz
AC240V ±10% (Australia) 50/60Hz

I-26 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
 Maximum Rated Power Consumption
3.5kVA
Electrolyte Measurement Unit (ISE)
Measures the densities of the Na, K and Cl ions using the ion-selective electrode. Throughput:
200 samples/hour
Optional Accessories

Forced Drainage Equipment Drainage Capability 58 liters/hour (50 Hz)

70 liters/hour (60Hz)
Drainage Head 4 feet
Printer 136-digit printer

Sampling Specifications
Sampling Mechanism, Micro-Syringe Type
Clot detection function added: If a clot is detected during sample aspiration and/or sample
dispense, an alarm occurs. The system cancels the sample aspiration and moves to next
sample.
Crash detection function added: If the probe tip bumps against a sample cup while it is
moving down, the system automatically stops the probe operation. The probe may not be able
to dispense accurately in the future if it was damaged. If the probe is damaged, refer to the
Maintenance Chapter for replacement procedures.
Sample Quantity Setting
2 to 50 µl/test: Can be set in increments of 0.5 µl.
Sample Dilution Quantity Setting
0 or 10 µl/test
Sample ID
Read from the barcode. One of the following barcode types can be selected: NW7, Code 39,
Code 128, 2 of 5 standard, 2 of 5 interleaved, and mixed. ISBT-CODE128 can be read only
if no other types are mixed.
Types of Sample Racks
Sample Rack Type The racks are visually classified according to color. The analyzer
recognizes the type of rack by the placement of magnets located on the bottom of the racks.

White: Routine sample rack

Red: Emergency sample rack
Yellow: Calibration sample rack
Green: Quality control sample rack
Blue: Reagent blank rack
Orange Repeat sample rack

Rack No. Read from the barcode..

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-27
August, 2002
 Sample Cup Types and Placement

Sample Cup Type Rack Outer STAT Inner STAT Remark

Ring Ring
Commercial conical cup ü ü
Micro sample cup ü Hitachi (707-0313)
Hitachi cup ü ü Hitachi (716-0425)
ACA cup ü ü ü
Kendall Ezee_Nested Micro cup ü 1270016000
1270013000
Evergreen Scientific Nested Micro Cup ü 127-1212-010
Commercial blood collection tube *1 ü ü ü *2
Inside diameter: 9mm to 15mm
Outside diameter: 11.5mm to 16mm
Length: 55mm to 100mm * 3

*1: The maximum diameter of a blood collection tube should be less than 17.5mm. An
adapter fitting to the outside diameter is required.
*2: Another adapter is required for tubes with a 12mm outside diameter.
*3: If the sample source is a blood collection tube, use only tubes with a total length
< 100mm.

Reaction Unit Specifications

Reagent Setup Method
Turntable type
Types of Reagent
Normal-density reagent, high-density reagent
Reagent Dispense Mechanism
Micro-syringe type
Crash detection function added: If the probe tip bumps against a sample cup while it is
moving down, the system automatically stops the probe operation. The probe may not be able
to dispense accurately in the future if it was damaged. If the probe is damaged, refer to the
Maintenance Chapter for replacement procedures.
Number of Reagent Steps
2 steps
Reagent Volume Setting Range
25 to 300 µl Can be set in increments of 1 µl.
Reagent Volume Dilution Setting Range
0, 10 to 250 µl
Mixing Method
Rotating mix bar
Reaction Container
Square, glass cuvette Capacity: 750 µl Light Path: 6mm

I-28 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
March 2, 2007
 Incubator Temperature Control
Dry bath type
Reaction Line
Rotating disk type 88 cuvettes/line.

Photometer Unit Specifications

Optical System
Polychromatic system
Wavelength Range

340 to 800 nm 13 wavelengths: 340, 380, 410, 450, 480, 520, 540,
570, 600, 660, 700, 750 and 800
nm
Light Source

Halogen lamp 12 V/20 W

Average life: 1,000 hours
Detector
Silicone photodiode array
Measurement Absorbance Range

0 to 2.5 10 mm light path conversion

Resolution of Photometry
0.0001OD
Data Processor Unit Specifications
Configuration of the Data Processor Unit

Hard disk 2 GBs or more

Memory capacity 64 MB
Floppy disk 3.5 inch (2HD 1.44 DOS format)
3.5 inch (2DD 720 kB DOS format)
Keyboard 106-key keyboard (DOS/V)
CRT High-resolution, color
CD-ROM drive
Printer
Standard printer: 136-digit dot matrix
Optional printer: 136 digit dot matrix

Analysis Processing Specifications

Assay Types
Single-end point assay, dual-end point assay, rate assay, fixed point assay, and electrolyte
method (ISE).
Object of Analysis
Blood serum, urine, CSF, or Plasma
Measurement Items

Colorimetry enzymes, lipids, proteins, sugars, nitrogen compounds, inorganic

matters, complements, TBA, etc.
Turbidimetry IgG, IgA, IgM, C3, C4, RF, CRP, ASO, transferrin, etc.
Latex agglutination RF, CRP, ASO, etc.
Homogeneous EIA (EMIT) DAU, TDM, etc.
ISE Na, K, and Cl

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-29
August, 2002
 Number of Tests that can be Analyzed Simultaneously
Maximum 38 tests/sample (R1 & R2)
Maximum 41 tests/sample (using ISE)
Throughput
Processing speed 400 tests/hour photometric / Maximum 800 tests/hour / ISE
Reaction Total Vol. 150 to 550 µl
Reaction time Maximum 8 min. 37.5 sec.
Reaction temp. 37 °C
Saved samples The capacity of sample storage on the hard disk is a maximum
of 30,000 samples or 90 indexes whichever comes first. The
maximum capacity of samples is 8,000 per index total including
routine, emergency, repeat, priority STAT and STAT repeat (serum,
urine, & other). Urine & other Sample type maximum is 999 each
type per index.
Emergency serum (E001-E999), emergency urine (UE001-UE999), & emergency other
(XE001-XE999) sample type maximum is 999 each type per index.
Repeat serum (H001-H999), repeat urine (HU001-HU999), & repeat other (HX001-
HX999) sample type maximum is 999 each type per index.
Priority STAT serum (P001-P999), priority STAT urine (UP001-UP999), & priority STAT
other (XP001-XP999) sample type maximum is 999 each type per
index.
STAT repeat serum (HP001-HP999), STAT repeat urine (HUP001-HUP999), & STAT
repeat other (HXP001-HXP999) sample type maximum is 999 each
type per index.
RB & Cal. Reagent Blank (R001-R999), Calibration (A001-A999) maximum is
999 samples per index.
QC (Q001-Q999) maximum is 999 each type per index. A maximum of
300 QC indexes are saved.
A maximum of 10,000 tests are
saved in [Routine], [Reaction
Monitor]. The data is still present
in old indexes after a new index
is created, up to 10,000 tests
maximum.
Floppy disk type HD 1.44 MB stores a maximum of 250 samples or 8 indexes,
whichever comes first.
Floppy disk type DD 720 KB stores a maximum of 120 samples or 8 indexes,
whichever comes first.

Input/Output Specifications
Data Display
Display characters: Alphanumerics
Data Input/Output to External Devices
Data exchange by RS232C or floppy disk

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August, 1998
November, 2002
Screen Menu Items
[Routine] menu
OLP4049E

(R) Routine (S) Start Condition

(T) Test Requisition (N) Normal
(R) Repeat
(Q) QC
(C) Calibration
(S) Retrieve Repeat Data
(R) Reaction Monitor
(C) Calibration Monitor (S) Calibration Curve
(T) Calibration Trace
(B) Reagent Blank Monitor
(P) Photocal Monitor
(Q)QC Monitor (D) Daily Control
(S) Day To Day Control
(T)Twin Plot
(E) Data Edit
(D) Data Management (E) Data Edit
(C) Data Correction
(W) Repeat Data Verification
(A) Data Report (R) Report
(D) Data List
(N) Online

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 OLP4050E
[Parameters] menu

(P) Parameters (Y) Common Test Parameters (A) Test Name

(B) Profile
(C) Round
(I) Specific Test Parameters
(H) Inter-Related Tests (A) Calculated Tests
(H) Checked Tests
(B) Sample Blank
(R) Repeat Parameters (C) Repeat Common
(I) Repeat Specific
(Q) QC Control (C) QC Common
(I) QC Specific
(S) STAT Table QC
(B) Calibration (C) Calibrator
(I) Calibration Specific
(S) STAT Table Calibration
(O) Online
(F) Format (P) Printer
(S) Requisition Format
(R) Report Format
(L) List Format
(C) Special (C) Contamination Parameters
(D) Data Check Parameters
(S) System (O) Option
(S) System
(P) Auto Power On
(W) Password
(L) Login Name
(U) User Menu
(M) Comment Masters

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August, 2002
[Auxiliary] menu
OLP4051E

(A)Auxiliary (S) Data Statistics

(C) Correlation Chart
(H) Histogram
(R) Reagent Consumption
(P) Parameters Management
(V) Calibration Verification
(B) Standby Set

OLP4052E
• [Maintenance] menu

(M)Maintenance (D)Data Operation (F)FD Data Management

(T)Set Date & Time

(D)Retrieve Data Base

(O)Offline Output

(M)PC Anywhere
(A)ANL Maintenance

(C)Consumable Management

(P)Periodic Maintenance

(M)Maker Maintenance (V)Program Version

(A)ANL Diag

(I)ISE Diag

(L)Alarm Log

(F)File Management

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AU400/AU400e User's Guide Chapter I Specifications I-33
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ISE Unit Specifications
Measurement Method
Diluted ion-selective electrode method
Measurement Items
Na, K and Cl ions in serum and urine
Throughput
200 samples/hour
Dispensed Sample Amount
serum: 20 µl urine: 25 µl
Dilution
Serum: 1:32.4 times (DI water 10µl, buffer solution 618 µl)
Urine: 1:31.4 times (DI water 10µl, buffer solution 750 µl)
Measurement Range
Item Serum Urine
Na 50 to 200 10 to 400
K 1.0 to 10.0 2.0 to 200
Cl 50 to 200 15 to 400
Unit: mmol/l
Calibration
Auto calibration
Measures the high-density reference solution and low-density reference solution and performs
two-point calibration.
Data Correction
Manual calibration correction (M-CAL) and auto calibration correction (A-CAL) are possible.
Drift Correction
Auto correction
Measures the potential of the MID solution for each sample and corrects the drift.
Electrode Type and Form
Type Form
Na Crown ether membrane
K Crown ether membrane
Cl Molecular oriented membrane
Reference electrode Liquid junction

I-34 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
5. Calculations
This chapter explains the following measurement calculation methods available in this system:
reagent blank (zero adjustment), end point assay, rate assay, fixed point assay, and sample blank
adjustment.

5.1 Reagent Blank (Zero Adjustment)

The reagent blank (zero adjustment) method is described below.

Reagent blank (Zero Adjustment)

To determine a measurement value (reaction OD), the reagent blank OD, at each photometric
point, is subtracted from the measurement unless the method is End 1, Rate 1, or Fixed 1. The
photocal data is subtracted from the measurement OD of a sample blank reaction that took place
with the reagent.
By performing a reagent blank (RB), the OD values at all photometric points, shown in the
following figure, are obtained and placed in the reagent blank table. The method parameters
determine which points are used. Prepare sample cups (for serum, other & urine) filled with
deionized water. Use a blue rack and place sample cups in position 1 for serum, position 4
for other and position 10 for urine. If the AU400 is used with identical reagent specifications
for serum/other/urine set to "none," place only one sample cup filled with deionized water in
position 1 of the blue rack.
To perform a (reagent blank) zero adjustment:
1. Set a sample cup that contains deionized water in place of a sample in the No. 1 position
on the blue rack. The system assays samples from the sample cup and determines the
reagent blank data (reagent blank OD value) by averaging 2 data samples, after excluding
the maximum and minimum data, if replicates are set equal to 4. The system can also be
set to 1, 2, 3, or 4. If one is selected, only one value is used. If two is selected, the two
OLP4089E
values are averaged. If three is selected, the two closest values are averaged.

OD Reagent Blank (Compared with water blank ; example of 2 step analysis)

Reagent OD value at the

Reagent OD value at the first point (first data)
last point
(second data)

Deionized water blank (photocal data)

Photometric point
P0 P1 P10 P11 P27
R1 Sample R2
R1 : First Reagent
R2 : Second Reagent

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AU400/AU400e User's Guide Chapter I Specifications I-35
August, 2002
 The following describes reagent data No. 1 and No. 2 printed. The P0 reagent OD (first 0
adjustment data) and last-point reagent OD (second 0 adjustment data) after the reagent blank is
completed. This is an example of a reagent blank printout.
Reagent Blank (OD)
No. Cup Pos. CA TP BUN CREA GLU LD AST
(Serum) 1 R001 0.0881 0.2330- 1.2820 0.0106 0.0664 0.3333 0.8596u
(Serum) 2 R001 0.0864 0.2332- 1.2661 0.0106 0.0646 0.3327 0.8582U
(Urine) 1 R001 0.0106
(Urine) 2 R001 0.0107

R001: R is the prefix for reagent blank. R001 is the first blue rack through the analyzer. The
second blue rack through the analyzer prints results labeled 1R002, 2R002. The maximum
number of reagent blanks per index is 999.
1R001: The OD at the first read point of the test (as set in [Parameters], [Specific Test
Parameters]). Generates y (over) or u (under) flags based on an acceptable OD range entered
in the individual parameter screen. For example: Data number 1 OD for serum TP is -0.2330.
Note that a negative sign follows the result. Data number 1 OD for serum AST is under the
acceptable range and therefore has generated a "u" flag.
2R001: The OD at the last read point of the test (as set in [Parameters], [Specific Test
Parameters]). Generates Y (over) and U (under) flags based on the acceptable OD range entered
in the individual parameter screen. For example: Data number 2 OD for urine CREA is 0.0107.
Data number 2 OD for serum AST is under the acceptable range and therefore has generated a
"U" flag.

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August, 2002
5.2 Endpoint Assay
This section describes the end point assay.

1-point Assay
This is a general end point assay that determines the reaction mixture OD from the OD measured
at a specified photometric position. The diagram below illustrates the case for one reagent only
with reagent volume equal to or greater than 150 µL. In this case the photometric position is 27,
positions 1-27 may be used. OLP4090E

Reaction mixture OD = OD (at specified position) - OD0(at position 0)

OD

R1 0 S 1 2 3 4 5 6 7 8 27
P0 Px

∆OD = Px (K1 X P0) when positive reaction

- {Px - (K1 X P0)} when negative reaction
where K1 = R1/(R1 + S)
P0, Px is OD at point 0 and end point

2-point Assay (self-blank method)

This end point assay provides a sample blank adjustment. The OD value before dispensing the
second reagent is eliminated as the sample blank. The OD value adjusted by the dilution factor
in the sample blank is subtracted from the OD measured after dispensing the second reagent.
Thus the OD associated with the serum can be removed, and greater measuring accuracy will be
obtained. The final OD value in this assay is given by the following expression:
K2 = {R1. V / (R1.V + R2.V + S.V)}
K3 = {(R1.V + S.V) / (R1.V + R2.V + S.V)}
Reaction OD value = (Px - K2 x P0) - (K3 x Pz - K2 X P0)
This calculation result is defined as the reaction OD value.

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 OD

R1 0 S 1 2 .... 9 10 R2 11 .... 27
R1.V S.V Pz R2.V Px

R1.V: First reagent dispense volume

R2.V: Second reagent dispense volume
P0 : Reagent OD value at the first point
Pz : Reagent OD value before dispensing the second reagent
Can be P1 - P10.
Px : Reagent OD value after dispensing the second reagent.
Can be P11 - P27.

Instruction
Twenty-seven is the last read point. There are no OD values or variables after this point.

End Assay (two test: blank/color method)

This method uses a blank test to measure serum turbidity or other interfering impurities. This
separate blank test OD is then subtracted from the measured OD of the color test to determine
the net reaction OD. Although this uses an additional blank test and reagent, this method may
improve measurement accuracy over that of some 2-point assays. OLP4092E

Reaction mixture OD = Color reaction channel OD (ODC) - Serum blank channel OD (ODB)

Color reaction channel

OD

ODC

Serum blank channel

ODB

0 0
R1 S R2 27

Instruction
To link the color reaction test and serum blank test, go to [Parameters],
[H - Inter-Related Tests], and [B - Sample Blank]. Enter the test number for the color reaction test. See
Section 5.5 Sample Blank for more information.

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AU400/AU400e User's Guide
August, 2002
5.3 Rate Assay
This section describes the rate assay.

Rate Assay
This assay determines the rate of absorbance variation per minute or change OD/min by
calculating the slope of the change by least squares in absorbance variations for all OD values
within the OD limits and included in the defined photometric points. Note at least three
consecutive OD values must be within the OD range limit. If no-lag-time is defined as yes, then
values before the defined first read point can be used for fast reactions.

Instruction 4093

The Y axis is the reaction OD and the X axis is the photometric read point or time in minutes.

OD

∆OD/min.

OD limit

R1 S R2 27

∆OD = ∆Pwi when the reaction is positive

-{ ∆Pwi } when the reaction is negative
∆Pwi is effective points slope (OD/min) by least squares
∆Pyi is effective points slope (OD/min -- self blank points) by least squares

Double Rate Assay

This assay determines the difference in rate of absorbance variation per minute or the change in
OD/min by calculating the difference in the slope of the change in absorbance variations from
the start to end points. Finally the system obtains the net OD/min of the reaction by subtracting
a blank rate from the reaction rate.
∆OD = ∆Pwi - (K1 X ∆Pyi) when the reaction is positive
-{ ∆Pwi - (K1 X ∆Pyi)} when the reaction is negative
where K1 = (R1 + S)/(R1 + R2 + S)
∆Pwi is effective points slope (OD/min) by least squares
∆Pyi is effective points slope (OD/min -- self blank points) by least squares
On the methodology sheet and in the test parameters, the start (Fst) and end (Lst) photometric
point for the rate, ∆OD(1)/min is defined as point 1 and for ∆OD(2)/min as point 2.

OD
DOD(1)
DOD(2)

R1 S Pz Pw R2 Py Px 27

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5.4 Fixed Point Assay
This section describes the fixed point assay.

Fixed Point Assay

This method determines the difference between the ODs at two specified positions.
∆OD = Px - Py when the reaction is positive
-(Px - Py) when the reaction is negative
where Px is last point OD
OLP4094E
Py is first point OD
Reaction mixture OD = ODB - ODA

OD

R1 S R2 A B 27

Double Fixed Assay (Self Blank)

∆OD = (Px - Py) - K1 X (Pw - Pz) when the reaction is positive

-((Px - Py) -K1 X (Pw - Pz)) when the reaction is negative
where K1 = (R1 + S)/(R1 + R2 + S)
Px, Py is OD at last and first point
Pw, Pz is OD at last and first self blank point
On the methodology sheet and in the test parameters, the start (Fst) and end (Lst) photometric
point for the reaction, ∆OD(1) is defined as point 1 and the blank ∆OD(2) as point 2.

OD
DOD(1)
DOD(2)

R1 S Pz Pw R2 Py Px 27

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Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
5.5 Sample Blank
This section describes the sample blank method using two inter-related tests.

Sample Blank
The sample blank method uses the specified Color Test and Blank Test as defined in [Parameters],
[Inter-related Tests], [Sample Blank].
OD calculated = color blank OD - [Reagent blank OD]
If a Color Test is selected, the Blank Test must be specified in the blank test field on the [Sample
Blank] screen. OLP10sb

STANDBY
?
1998/11/03 10:10

Sample Blank

Color Test Blank Test

1. TBILC TBILB
2. DBILC DBILB
3. None Test Name:
4. None Test Name:
5. None Test Name:
6. None Test Name:
7. None Test Name:
8. None Test Name:
9. None Test Name:
10. None Test Name:

Help Exit Set

ALARMCLEAR

If a Color Test is selected on the screen, a corresponding Blank Test must be specified.
Once these tests are inter-related in the parameters shown above, and the Color Test is ordered, the
test specified as the Blank Test will automatically be ordered.

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AU400/AU400e User's Guide Chapter I Specifications I-41
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5.6 LIH
Before the LIH level can be determined, the OD limits for each level that produce the flags must
be determined and programmed. Select appropriate samples that represent the five cutoff levels
of each interferant: lipemia, icterus (bilirubin), and hemolysis.

See Also
For LIH procedures, refer to [Parameters], [Specific Test Parameters] located in the Software Chapter.
Program three tests with the following wavelength pairs: 410 nm and 480 nm (P1-P0) LIH1,
570 nm and 600 nm (P2-P0) LIH2, 660 nm and 800 nm (P3-P0) LIH3 to allow the determination
of the ODs at the six wavelengths used in the LIH OD calculations. These three tests may have
sample volume equal to 18.0 ul, sample diluent volume equal to 0.0 and reagent 1 volume plus
reagent 1 diluent volume equal to 282 ul. (It is highly recommended that these volumes be equal
to the numbers programmed in LIH.) Test No. 96 LIH MUST BE selected and use normal saline
as Reagent 1. Set the calibration for these three temporary tests to Cal type 1 MB with factor
equal to 1000 and decimal to 1. Set the Method to End, Reaction to + and reagent OD limits to
-2.0 to 2.5.
Run replicates of the cutoff levels of each type of interferant. Print the OD data for each sample
and each temporary LIH test. See [Routine], [Reaction Monitor] to select "List Print of Display
Data."
• To calculate the lipemia reaction OD, the equation is (OD 660-OD 800) both at P3 minus
reagent blank OD (660-800).
• To calculate the icterus reaction OD, the equation is (OD 480 at P1 - OD 570 at P2)
minus (OD 600 at P2 - OD 800 at P3) minus reagent blank.
• To calculate the hemolysis reaction OD, the equation is (OD 410 - OD 480) both at P1
minus (OD 600 at P2 - OD 800 at P3) minus reagent blank.
The final values for lipemia, icterus and hemolysis (LIH) are compared to the OD levels set in
[Parameters], [Specific Test Parameters] after the following adjustment is made:
LIH check data = Reaction OD x (R1 Volume + R1 Dilution Volume + Sample Volume +
Sample Dilution Volume)/(300) x 18/ (Sample Volume + Sample Dilution Volume)
OR
Reaction OD X R1 vol. + R1 dil. vol. + sample vol. + sample dil. vol. 18
LIH check data = 300
X Sample vol. + sample dil. vol

For example: If the lipemia, icterus and hemolysis reaction ODs are less than the OD limit set
for Level + for each of these, then the LIH flags are printed as LIP N, ICT N and HEM N. If a
reaction OD is equal to or greater than the Level OD limit, but less than the next higher Level
OD limit, then the appropriate Level flag of + to +++++ is printed. If the reaction OD limit at
any of the 6 wavelengths is greater than 2.5 or if OD 410 exceeds 2.0 then ABN for abnormal is
printed for Software versions up to 8.3. Software version 9.0 differentiates between ABN L for
abnormal low and ABN H for abnormal high depending on how high or low the OD wavelength
reaction limit is exceeded. If the sample was not analyzed for LIH it is left blank.

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April 18, 2011
LIH Determination (up to version 8.3)
The following flow chart represents how the system determines detailed LIH information after
analysis for software versions up to 8.3. The flags are judged N, +, ++, +++, ++++, +++++,
ABN.

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AU400/AU400e User's Guide Chapter I Specifications I-42a
April 18, 2011
LIH Determination (Version 9.0 and later)
The following flow chart represents how the system determines detailed LIH information after
analysis, if the LIH check function in software version 9.0 is enabled. The flags now report
N, +, ++, +++, ++++, +++++, ABN H, ABN L.
The ABNORMAL flag in previous software versions has been extended to report ABN H
(Abnormal High) and ABN L (Abnormal Low).
To upgrade to software version 9.0, and enable this function, contact your Beckman Coulter
Technical Services.

I-42b Chapter I Specifications

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April 18, 2011
6. Quality Control
A wide variety of quality control techniques are available. On this instrument, -R control
(which is the most widely used quality control technique), and multi-rule quality control (which
prevents detection of insignificant errors) are designed as a part of the standard software. This
section describes multi-rule control. For information about the - R control, refer to the
Software Chapter.

Twin Plot Control

OLP4086E
In quality control, usually a normal control and an abnormal control are used. The following
figure shows a two-dimensional plot with the normal control on the x-axis and the abnormal
control on the y-axis.
Abnormal
region sample

+2SD
(1) is an example of normal
control results ~ - 1SD below
the mean and abnormal
MEAN control > -2SD below the mean.

-2SD

(1)

-2SD MEAN +2SD Normal

Control

When the normal and abnormal controls are within control limits, but both controls are
repeatedly biased high or low, investigate system errors by performing a reagent blank or
calibration. If the problem persists reagent deterioration may be the cause; replace reagent
calibrate and repeat controls.
The twin plot offers the advantage of classifying problems as either a system error or a random
error. To be effective it should be combined with multi-rule control procedures to determine if
drift is occurring or if the reagent is unstable.

Multi-Rule Control
In the -R control, a control error is checked by examining multiple control rules, but it is
difficult if not impossible to confirm numerous tests on a real-time basis. The multi-rule control
technique makes it possible to speedily cope with an error real-time, as this control method
notifies the worker of just which rule an error (when generated) violates based on an error flag.
When using this control technique, test samples of both the normal range and the abnormal
OLP4087E
range. Refer to the following figure:
Control OUT-OF-CONTROL REJECT RUN
Data
Yes Yes

No No No
12S Trend IN-CONTROL Trend

Yes No

No No No No
13S 22S R4S 4 1S 10x

Yes Yes Yes Yes Yes

OUT-OF-CONTROL REJECT RUN

Standard of judgement based on the multi-rule Shewhart technique.

(Logic diagram Applicable to Control Rules)

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Explanation of Symbols for Multi-Rule Control and the Corresponding Error Flag:
1 Error Flag
12S is a judgment level for determining if one control result has exceeded the mean ±2 SD
and that five subsequent judgment levels (shown in the table on the following page) are
sequentially checked to see if there is any violation of the applicable rules.
2 Error Flag
13S is a judgment level for determining if one control result has exceeded the control limit
‘mean ±3 SD.’ When it exceeds the control limit, quality control has not been attained. If it
does not exceed the control limit, an inquiry for judgment is made to the next judgment level
22S.
3 Error Flag
22S is a judgment level for determining if two consecutive control results have exceeded the
control limit ‘mean ±2 SD’ in one direction. If the control limit is exceeded, then quality
control has not been attained. If it does not exceed the control limit, see the next judgment
level R4S.

Tip
The term “consecutive” applies to either one identical control substance or a high-concentration and low-
concentration control substance.

4 Error Flag
R4S is a judgment level for determining if two control results, with high and low
concentrations, exceed the control limit “mean + 2SD” and if the other exceeds the control
limit of “mean – 2SD.” If the data is out of the control limit, then quality control has not
been attained. If the data is within the control limit, see the next judgment level, 41S.
5 Error Flag
41S is a judgment level for determining if four consecutive control results exceed the control
limit of either ‘mean +1 SD’ or ‘mean -1 SD.’ If they have exceeded the limit, then quality
control has not been attained. If they have not exceeded either control limit, an inquiry is
made to the next judgment level 10 .
6 Error Flag
10 is a judgment level for determining if 7 - 10 consecutive control results fall on one side
of the control mean (above or below). If they have exceeded the control limit, then quality
control has not been attained. If they have not exceeded the control limit, then quality control
has been attained.
7 Error Flag
Trend: Indicates that five through ten QC data points (user defined) exhibit steadily
increasing or decreasing values. (A number 7 error flag is generated.)

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August, 2002
 If an error is encountered through the 6 rules described above, an abnormal data flag is printed
as a list or displayed on the screen. The abnormal QC data flags and possible causes are
described below:
Error Flag Control Limit Cause of Error
Single Rule
1. (Data exceeds QC range in single rule) Exceeds QC range
Multi Rule
2. (Data exceeds the 3SD control limit) Exceeding 13S Random error
3. (Data continuously exceeds the 2SD Exceeding 22S Systematic error
control limit)
4. (Data exceeds the R4S control limit) Exceeding R4S Random error
5. (Data exceeds 1SD control range) Exceeding 41S Systematic error
6. (Data over/under the last 10 averages) Exceeding 10 Systematic error
7. (Data over/under range in multi rule)

See Also
For more information on Error Flags, refer to the Error Flags Chapter.
For more information on Quality Control, many sources are available such as the following:
Tietz, N. W., ed. "Westgard Multi-Rule Control Chart," Fundamentals of Clinical Chemistry,
Third Edition. Philadelphia: Harcourt Brace Jovanovich, Inc., 1987. 246-247.

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 OLP4088E

Example of Control Errors According to the Multi-Rule Control are shown below

High value sample 2 2S : Systematic error extending over the 2 type concentration range 1 3S
R 4S : Random error
3SD
2SD
1SD
Mean
Ð1SD
Ð2SD
Ð3SD

2 2S : Systematic error in the high concentration region Day

4 1S : Systematic error extending over the 2 2S : Systematic error extending over

Low value sample the 2 type concentration range
2 type concentration range

3SD
2SD
1SD
Mean
Ð1SD
Ð2SD
Ð3SD

10x : Systematic error in the low concentration region Day

13S : Random error

The following describes the possible causes and corrective actions for random errors and
systematic errors shown in the figure above.
Random Errors
• Dispensing Problem (sample, reagent)
For troubleshooting information refer to the Troubleshooting Chapter.

• Photometer Lamp Problem

For troubleshooting information refer to the Troubleshooting Chapter.

• Reagent Deterioration
For troubleshooting information refer to "Troubleshooting Data Flags" in the Error Flags
Chapter.

• Poor Quality Control Sample

Mistaken sample, different lot, etc.

See Also
For troubleshooting information refer to the Troubleshooting Chapter.
• Periodic Maintenance Not Performed Properly
For information on scheduled maintenance refer to the Maintenance Chapter.

• Poor Mixing
For troubleshooting information refer to the Troubleshooting Chapter.

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 Systematic Errors
• Incorrect Calibration
Incorrect handling or preparation of calibration samples.

See Also
For troubleshooting information refer to "Troubleshooting Data Flags" in the Error Flags Chapter.
• Deteriorated Reagent
For troubleshooting information refer to "Troubleshooting Data Flags" in the Error Flags
Chapter.

• Temperature Problems
For temperature specifications refer to the Specifications Chapter.

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I-48 Chapter I Specifications
Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002
7. AU400 Terminology
Terms frequently used for the AU400 are explained below:

W1
Abbreviation for automatic wash of cuvettes. If analysis was stopped, a W1 is performed to
remove the sample remaining in the cuvette and the cuvette is washed.

W2
Abbreviation for automatic wash of cuvettes and tubing. Perform a W2 weekly. After performing
W2 be sure to perform the photocal measurement.

See Also
For information on performing a W2, refer to the Maintenance Chapter.

Photocal Measurement
To obtain appropriate analysis results, cuvettes are checked for stains or scratches. Confirm
the photocal data obtained from a photocal measurement using the analysis status keys and the
[Photocal Monitor] screen.

See Also
• For detailed information on performing a photocal, refer to the Maintenance Chapter.
• For detailed information about analysis status keys, refer to the Software Chapter.
• For detailed information about the [Photocal Monitor] screen, refer to the Software
Chapter.

Profile
The profile screen sets up test panels. The individual tests are assigned for each profile. Profile
No. 99 is assigned to the One Touch Mode. Therefore, this profile must be used for the most
frequently used analysis tests. Set the profiles by choosing [Profile] from the [Parameters]
screen.

Round
A round is a category in which a set of analysis tests used for similar analysis are grouped
together so that specific analysis tests can be accessed quickly. Set the desired round by
choosing [Round] from the [Parameters] screen. Example: Designate the analysis tests used
for the routine analysis to Round 1, and the analysis tests used for drug analysis to Round 2.
Perform routine analysis under Round 1 and switch to Round 2 for drug analysis as required.

ACAL
Abbreviation for auto-calibration. It represents the automatic creation of calibration curves.
A calibration curve is automatically created using the yellow rack. It is mainly used for the
analysis tests in the end point assay method.

MCAL

Automated Chemistry Analyzer

AU400/AU400e User's Guide Chapter I Specifications I-49
August, 2002
 Abbreviation for manual calibration. It defines manual creation of calibration curves. A
calibration curve is created by manually entering the individual data. It is mainly used for the
analysis tests in the rate assay method.

RB
Abbreviation for reagent blank. In routine analysis the reagent blank serves as the reference
value for the reagents at each photometric point of individual analysis tests. It also becomes the
Y-segment first data point of calibration curves created by ACAL.

LIH Testing
Performs a test for lipemia, icteric, and/or hemolysis in serum. LIH is the symbol used for
testing lipemia (L), icterus (I), and hemolysis (H). Checks the level of interference present that
may make the sample analysis result abnormal.

END
This is one of the methods available for individual test parameters of the system. In the end
point assay method, it defines the analysis method that uses the water blank absorbance as
the reference for measurement data at each photometric point. The water blank absorbance is
obtained from the photocal measurement. “END” is a method test for individual test parameters.
This is another end point assay method identical to “END 1.” It uses reagent blank absorbance
as the reference for measurement data at each photometric point. END 1 does not use the
reagent blank.

RATE
Set-up test for individual test parameters of the system. In the rate assay method, it defines
the analysis method that uses water blank absorbance as the reference for measurement data at
each photometric point. Water blank absorbance is obtained from the photocal measurement.
“RATE” is a set-up test for individual test parameters. RATE 1 is another rate assay method
identical to “RATE.” RATE uses reagent blank absorbance as the reference for measurement
data at each photometric point. RATE 1 does not use the reagent blank.

FIXED
Set-up test for individual test parameters of the system. In the fixed point assay method, it
defines the analysis method that uses water blank absorbance as the reference for measurement
data at each photometric point. Water blank absorbance is obtained from photocal measurement.
“FIXED” is a set-up test for individual test parameters. This is another fixed point assay method
identical to “FIXED 1.” It uses reagent blank absorbance as the reference for measurement data
at each photometric point. FIXED 1 does not use the reagent blank.

NO-LAG_TIME
For example, if analysis for a pathologic human sample using this system ends too quickly due
to rapid reaction, two or more photometric points of effective analysis data may not be obtained.
If this is the case, the system can be set-up to calculate the analysis result using the data in the
lag phase. Used for one of the analysis tests in the rate assay method.

I-50 Chapter I Specifications

Automated Chemistry Analyzer
AU400/AU400e User's Guide
August, 2002