BIOSTAT® Qplus

12-fold Screening Fermentor | Bioreactor

Features
– Space saving tower design
– Graphical user interface with touch
Screening Fermentor|Bioreactor System

screen operation
– Fully independent vessel control
– Integrated thermostat system
– Integrated gassing system with O2 supple-
mentation for microbial packages
– Integrated 4-gas mixing system with
Sparger and Overlay aeration for cell
culture packages
– Top drive agitation system with
maintenance-free motor
– Pre-configured software for up to 12-fold
operation
– Control of pH, DO, temperature, foam|level,
substrate addition, gas mixing and gas
flow rate
– Scaleable vessel design
– Optional Turbidity or Redox measurement
– Easy upgrade of cell culture packages for
multipurpose use
System Description The BIOSTAT® Qplus is ideal for: – Inclusive Supervisory Process control
The BIOSTAT® Qplus is a new generation of – Process development software
Fermentor|Bioreactor systems designed for – Process optimization
parallel operation with high throughput – Up- and Down-scale experiments
capability. – Strain and Cell line characterization
– Quality control
The combination of a newly engineered – Parallel production process control
control system launches small scale cultiva-
tion vessels into a new era of biotechnology Applications
screening. The BIOSTAT® Qplus has the – Growth studies of microbial, mammalian,
capability to control fully independently insect and plant cells
up to twelve culture vessels with minimal – Culture media composition and
manual operation. optimization
– Upscale migration, i. e. transition from
Application-driven configured packages shaking flasks
for microbial and cell culture provide – Downscale of production process for
everything to get started immediately. process optimization
The BIOSTAT® Qplus is available with – Small scale protein and Mab expression
scaleable culture vessel working volumes – High cell density cultivation
of 0.5 L and 1 L.

The BIOSTAT® Qplus is delivered with a

completely configured software package.
A 3-fold, 6-fold, 9-fold system can be easily
extended with a 3-fold vessel |supply tower
extension kit up to a 12-fold system without
expensive software extensions.

For further enhancement of system perform-

ance, a powerful supervisory process control
software MFCS | DA for extended visualiza-
tion, data acquisition and trend display is
included.
Technical Specification

Space requirements|Environmental conditions Dimensions

Bench space requirement|weight|0.5 L culture vessel
3-fold | 6-fold | 9-fold | 12-fold [W + H + D] 850 | 1320 | 1860 | 2400 + 900 + 750 [mm] | 95 | 155 | 216 | 278 [kg]
Bench space requirement|weight|1 L culture vessel
3-fold | 6-fold | 9-fold | 12-fold [W + H + D] 1100 | 1800 | 2550 | 3300 + 900 + 800 [mm] | 114 | 194 | 274 | 355 [kg]
Space requirement autoclave d + H 0.5 L
per culture vessel | 3-fold vessel assembly with tray 170 + 340 | 480 + 340 [mm]
Space requirement autoclave d + H 1 L
per culture vessel | 3-fold vessel assembly 240 + 500 | 460 + 500 [mm]
Ambient temperature|relative humidity (non condensating) 5–40°C|85%
Control Tower
Housing material Stainless steel AISI 304
Display Touch Screen 15"
Resolution 1024 + 600 dpi
Communication Control Tower|Supply Tower |Host Ethernet|Ethernet
Measurement ranges
Agitation motor speed 50 –1200 rpm
Temperature 0 – 150 °C
pH 2 – 12
pO2 0 – 100%
Turbidity (optional) 0 – 6 AU
Redox (optional) –1000–1000 mV
Supply Tower Microbial Version Cell Culture Version
Housing material Stainless steel AISI 304
Gassing System Air areation with O2 supplementation 4-gasmixing for Air N2, O2 CO2
Sparger outlet Sparger and Overlay outlet
Outlet design Hose connector OD 6 mm Hose connector OD 6 mm
Flowmeter
Gas flow range "Sparger" 0.5 L|1 L 0.1–1|0.16–1.6 [l/min] 0.005–0.05|0.016–0.166 [l/min]
Gas flow range "Overlay" 0.5 L|1 L –– 0.05–0.5|0.16–1.6 [l/min]
Accuracy +/– 5% FS
Thermal Mass Flow Controller (optional)
Flow range 0.03–1.5 slpm 0.003–0.150 slpm
Accuracy +/– 1% FS
Integrated Pumps
Pump head Watson Marlow 102R
Rotation speed 20 rpm
Flow rate integrated pumps 0.04–33.2 [ml/min] (tube dependent)
Thermostate System
Temperature control range 8 °C above cooling water to 60 °C
Connections to culture vessel Quick couplings
External Connections per Vessel
Feed pump connection|External input 0 – 10 V|0 – 10 V
Culture vessel 0.5 L 1L
Design Jacketed glass vessel with stainless steel head pleate
Total volume 0.75 L 1.6 L
Working volume 0.15–0.5 L 0.4–1 L
Headpleate ports total 19 mm | 12 mm | 6 mm –|6|4 3|2|6
pO2 electrode Polarographic
pH electrode Gel filled
Temperature probe Pt100
Material (product wetted parts) Borosilicat glass|Stainless steel AISI 316 L|EPDM
Volume storage bottles 250 mL
Utilities Requirements|Housing Connection
Power supply 120 VAC or 230 VAC
Gasses Controlled @ 1.5 barg dry, particle and oil free|hose connector OD 6 mm
Water Controlled @ 2 barg|hose connector OD 10 mm
Drain Gravity drain with zero backpressure required |hose connector OD 10 mm
Ordering Information

Description BIOSTAT® Qplus Microbial Version BIOSTAT® Qplus Cell Culture Version
Culture vessel 0.5 L 1L 0.5 L 1L
Cat. No. 120 VAC
BIOSTAT® Qplus 3-fold version RQ-3M5DLOTRDG1 RQ-3M01LOTRDG1 RQ-3C5DLETSDG1 RQ-3C01LETSDG1
BIOSTAT® Qplus 6-fold version RQ-6M5DLOTRDG1 RQ-6M01LOTRDG1 RQ-6C5DLETSDG1 RQ-6C01LETSDG1
BIOSTAT® Qplus 9-fold version RQ-9M5DLOTRDG1 RQ-9M01LOTRDG1 RQ-9C5DLETSDG1 RQ-9C01LETSDG1
BIOSTAT® Qplus 12-fold version RQ12M5DLOTRDG1 RQ12M01LOTRDG1 RQ12C5DLETSDG1 RQ12C01LETSDG1
3-fold MO upgrade to max 12 fold system
without digital controller RQ-3M5DLOTRDG1E RQ-3M01LOTRDG1E RQ-3C5DLETSDG1E RQ-3C01LETSDG1
Cat. No. 230 VAC
BIOSTAT® Qplus 3-fold version RQ-3M5DLOTRDG2 RQ-3M01LOTRDG2 RQ-3C5DLETSDG2 RQ-3C01LETSDG2
BIOSTAT® Qplus 6-fold version RQ-6M5DLOTRDG2 RQ-6M01LOTRDG2 RQ-6C5DLETSDG2 RQ-6C01LETSDG2
BIOSTAT® Qplus 9-fold version RQ-9M5DLOTRDG2 RQ-9M01LOTRDG2 RQ-9C5DLETSDG2 RQ-9C01LETSDG2
BIOSTAT® Qplus 12-fold version RQ12M5DLOTRDG2 RQ12M01LOTRDG2 RQ12C5DLETSDG2 RQ12C01LETSDG2
3-fold upgrade to max 12 fold system
without digital controller RQ-3M5DLOTRDG2E RQ-3M01LOTRDG2E RQ-3C5DLETSDG2E RQ-3C01LETSDG2E
Control Unit
Digital controller
15’’ color display with touch screen • • • •
pH and pO2 calibration routine
Single probe and group calibration • • • •
Control capabilities listing per vessel
Temperature, pH, DO (multi stage cascade),
Stirrer speed; Foam|Harvest, Substrate • • • •
Supply Tower
Gasmixing (integrated) O2-Enrichment Exclusive Flow
Rotameter for Sparger|Overlay [l/min] •|— — •|•
Solenoid Valve for O2-Enrichment • 4-gas mixing
Gasmixing of Air, O2, N2, CO2 —— •
Mass Flow Controller Total Sparger Flow • 0.03 – 1.5 [l/min] 8848605 • 0.003–0.150 [l/min] 8848606
Peristaltic pumps (integrated) 3 per vessel 3 per vessel
Thermostat system for independent
vessel temperature control • •
Tubing, O-Ring (spare set) • •
Supervisory Process Control Software
MFCS | DA for data storage • •
Culture Vessel Listing per Vessel Jacketed UniVessel®
3-fold culture vessel tray • —— • ——
Individual culture vessel stand —— • —— •
Stirrer shaft with Single Mechanical Seal • • • •
6-blade disk impeller 2 2 — —
3-blade segment impeller — — 1 1
3 shared storage bottles 250 mL with tray • — • —
3 storage bottles 250 mL with bottle holder — • — •
Air Inlet and Exhaust filter 2 2 3 3
Aeration tube with Ring-sparger • • — —
Aeration tube with Micro-sparger — — • •
Innoculation|addition kit • • • •
Exhaust Cooler • • • •
Addition fitting 2 + 3-way 4-way 2 + 3-way 4-way
Sample-|Harvest pipe • • • •
Manual sampler • • • •
Temperature probe • • • •
pH Electrode, cable • • • •
DO Electrode, cable • • • •
Level|Foam sensor, cable • • • •

• = included, — = not included, — — = unavailable, • = option

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