Operating Instructions

METTLER TOLEDO
DE40, DE50 and DE51 Density Meters

V2.00
Contents Page

1. Introduction ................................................................................................... 6

2. Safety measures ............................................................................................ 7

3. The density .................................................................................................... 8

3.1 Definition .......................................................................................................... 8
3.2 Measurement principle ..................................................................................... 8
3.3 Construction of the measuring cell and temperature control.......................... 11

4. The instrument ............................................................................................ 12

4.2. Keyboard ........................................................................................................ 14
4.3 Display ........................................................................................................... 17

5. Getting started ............................................................................................. 18

5.1 Setting up the instrument ............................................................................... 18
5.2 Performing the first adjustment (calibration) .................................................. 19
5.3 Perform a measurement ................................................................................ 23

6. Sample (Number and ID) and Sample File ................................................ 26

6.1 Sample File (sample changer) ....................................................................... 26

7. Report (password protected) ..................................................................... 29

8. Measuring parameter (password protected) ............................................ 30

8.1 Method name ................................................................................................. 30
8.2 Measuring temperature .................................................................................. 31
8.3 Measurement stability .................................................................................... 31
8.4 Limit time ........................................................................................................ 32
8.5 Sequence (sampling, drain, rinse, purge) ...................................................... 32
8.6 Adjustment mode (Calibration selection) ....................................................... 34
8.7 Explanation of auto-sampling and auto-draining ............................................ 35

9. Calculation parameters (password protected) ......................................... 36

9.1 Density: d, d(t/4), d(t/t) ................................................................................... 36
9.2 Result (Density, Concentration, BRIX, ALCOHOL, USER TABLE) ................ 36
9.3 Temperature compensation ............................................................................ 41
9.4 Decimals ........................................................................................................ 42

METTLER TOLEDO DE40/50/51 Density Meter 3

Contents

Page

10. Functions (password protected) ............................................................... 43

10.1 Data File (Function 0) ..................................................................................... 43
10.2 Sample File (Function 1) ................................................................................ 46
10.3 Method File (Function 2) ................................................................................ 46
10.4 Changer (Function 3) ..................................................................................... 46
10.5 Factor (Function 4) ......................................................................................... 47
10.6 Set check (Function 5) ................................................................................... 47
10.7 Periodic (Function 6) ...................................................................................... 51
10.8 Auto Start (Function 7) ................................................................................... 51
10.9 CARD Utility (Function 8) ............................................................................... 52
10.10 Memory Clear (Function 9) ............................................................................ 53

11. Setup (password protected) ....................................................................... 54

11.1 Interface (Setup 0) ......................................................................................... 54
11.2 Date and Time (Setup 1) ................................................................................ 55
11.3 Name (Setup 2) .............................................................................................. 56
11.4 Serial/Version number (Setup 3) .................................................................... 56
11.5 LCD Contrast (Setup 4) .................................................................................. 57
11.6 International (Setup 5) .................................................................................... 57
11.7 Lock with password (Setup 6) ........................................................................ 58
11.8 Beep (Setup 7) ............................................................................................... 58
11.9 Temperature calibration (Setup 8) .................................................................. 59

12. Memory Cards (DE40, DE50 and DE51) .................................................... 60

12.1 Data Memory Cards: data, methods, sample files ......................................... 60
12.2 Save measured data ...................................................................................... 61
12.3 Clear measured data ...................................................................................... 62
12.4 Load measured data ...................................................................................... 63
12.5 Save method .................................................................................................. 64
12.6 Clear internal method ..................................................................................... 65
12.7 Load method .................................................................................................. 66
12.8 Save sample file ............................................................................................. 68
12.9 Clear internal sample file ................................................................................ 68
12.10 Load sample file ............................................................................................. 69

4 METTLER TOLEDO DE40/50/51 Density Meter

 Contents

13. Application Memory Cards: BRIX, ALCOHOL, API, VISCOSITY CORREC-

TION, USER TABLES .................................................................................. 70
13.1 How to download an application (Install table) ............................................... 70
13.2 How to select the application ......................................................................... 71
13.3 How to retrieve an application (Uninstall table) .............................................. 73

14. Description of the special applications:

BRIX, ALCOHOL, API, VISCOSITY CORRECTION, COKE, USER TABLES 75
14.1 API software ................................................................................................... 75
14.2 Alcohol software ............................................................................................. 75
14.3 BRIX software ................................................................................................ 76
14.4 Viscosity correction ........................................................................................ 76
14.5 Coke (Cola drinks) ......................................................................................... 77
14.6 Coefficients for conversion to various concentration data .............................. 77

15. Automatic sampling pump ASU-DE (optional) ........................................ 78

15.1 Safety measures ........................................................................................... 78
15.2 Installation ..................................................................................................... 79

16. Error Messages and Malfunctions ............................................................. 82

16.1 Error Messages .............................................................................................. 82
16.2 Malfunction ..................................................................................................... 83

17. Maintenance ................................................................................................ 84

17.1 Changing internal battery ............................................................................... 84
17.2 Power line voltage, power line, fuse ............................................................... 84
17.3 Replacing the silicagel ................................................................................... 84
17.4 Replacing the tube nozzles ............................................................................ 84

18. Standard and optional equipment ............................................................. 85

18.1 Standard equipment ....................................................................................... 85
18.2 Optional equipment ........................................................................................ 86

MemoCard DE40/50/51 (Short Instructions) .............................................................. 88

Annex 1: Density table of dried air ............................................................................ 89

Annex 2: Density table of pure water ........................................................................ 90

Specifications ............................................................................................................... 91

METTLER TOLEDO DE40/50/51 Density Meter 5

Introduction

1. Introduction

The DE40 and DE50/DE51 Density Meters measure the density d of gases, liquids and
solutions using the oscillating body method. They are distinguished by their temperature
range and their measurement accuracy. All three instruments:
• come with five certified water standards.
• measure gases, liquids and solutions with densities (d) in the range 0 to 3 g/cm3.
(DE40: with 0.0001 g/cm3; DE50/DE51: with 0.00001 g/cm3).
• need a minimum amount of substance (1.2 millilitres) for measurement.
• keep the temperature of the sample being measured constant thanks to a built-in thermostat
control (DE40: between 4 and 90 °C, DE50/DE51: between 4 and 70 °C).
• have 10 methods stored in their memory, which you can change to suit your measurements.
t
• show the density relative to water (specific gravity) at 4 °C (d 4 ) or at the measurement
t
temperature (d t ).
• calculate the concentration of a solution using tables or entered data of measured standard
solutions.
• calculate the density of a solution for a desired temperature using an entered temperature
function.
• have a built-in check function that allows to periodically check the accuracy of the instrument
without changing the instrument settings.
• store a maximum of 100 measured values.
• perform statistical evaluations (mean value, standard deviation, relative standard deviation)
and allow the recalculation of results.
• use Memory Cards for data storage, sample files and method transfer.

Special Application Memory Cards are available for all three instruments which allow the
automatic conversion of the experimental data into BRIX degrees, or alcohol concentration.
A special API software converts the density of oil products measured at elevated
temperatures automatically into the density at 15 °C (or 60 °F).

You can use an automatic sampling unit to perform continuous measurements; the built-in
RS-232C interface allows attachment of a computer to transfer experimental data. Print out
the entered parameters and data as well as results on a printer (option).

You can connect your instrument to a RE40 or RE50 refractometer to perform simultaneous
measurements of density and refractive index with one sample.

Connection of a SC24 sample changer allows fully automated measurements, even in

combination with a refractometer (RE40 or RE50).

Note: Examples of the density given in these Operating Instructions are for the DE50/DE51.

6 METTLER TOLEDO DE40/50/51 Density Meter

 Safety measures

2. Safety measures

Measures for your safety

– Ensure that you plug the power cable supplied into a socket which is
grounded! In the absence of grounding, a technical fault could be lethal.
– Switch the instrument off and disconnect the power cable if you change
blown fuses! An electric shock could be lethal.

– Never work in a hazardous area! The housing of the instruments is not gas
tight (explosion hazard through spark formation, corrosion by gas diffusion
into the instruments).

– Measure readily inflammable, toxic and corrosive substances in a fume

hood and comply with the usual lab regulations!

Measures for operational safety

– Check the set operating voltage before you switch on the instrument! The
instrument will be damaged if the operating voltage does not match the line
voltage.
– Do not clean the measurement tube with concentrated sodium hydroxide
(caustic soda, NaOH) or hydrofluoric acid (HF)! Both substances chemi-
cally attack the measurement tube.
– Use only fuses of the specified type if you need to change them!
– Exclude the following environmental influences:
• powerful vibrations.
• direct sunlight.
• high atmospheric humidity.
• temperatures below 5 °C and above 35 °C.
• powerful electric or magnetic fields which influence the power supply
through large load fluctuations!

METTLER TOLEDO DE40/50/51 Density Meter 7

Density

3. The density

3.1 Definition
The density ρ is the quotient of the mass m and the
volume V of a substance (mass density). As the m
density depends primarily on the temperature, the ρ= [kg/m3] or [g/cm3]
V
latter must always be specified.

The relative density is the ratio of the density ρ of a

substance to the reference density ρο of a reference ρ
d= ρ [1]
substance under conditions which must be 0
specified separately for both substances.

Note: The DE40/DE50/DE51 calculate the relative density: the density of water at 4 °C
(dtt ) or at the measurement temperature (dtt ) is available as a reference. For this
purpose, the density of pure water as a function of temperature is stored in the
instruments.

3.2 Measurement principle

The density measurement of the DE40 and the Amplitude
DE50/DE51 is based on the electromagnetically
induced oscillation of a glass U-tube.
One complete back and forth movement of a vibration
is a period, its duration is the period of oscillation T. Time
The number of periods per second is the frequency f.
Oscillation
Each glass tube vibrates at a so-called characteristic period T
or natural frequency. This changes when the tube is
filled with substance. The frequency is a function of
the mass. When the mass increases, the frequency
decreases, in other words period of oscillation T f= 1 [s-1]
T
incrases.
A magnet is fixed to the measurement tube in the
DE40/DE50/DE51 which is made to oscillate by a
transmitter. A sensor measures the period of
oscillation T.
Magnet

Oscillator/ Measurement tube of defined

Vibration meter volumetric capacity

8 METTLER TOLEDO DE40/50/51 Density Meter

 Density

The period of oscillation T is obtained from the equation:

ρVc + mc ρ = Density of sample in measurement tube [g/cm3]

T = 2π Vc = Volume of sample (capacity of tube) [cm3]
K
mc = Mass of measurement tube [g]
K = Measurement tube constant [g/s2]
It follows that

K 2 m
ρ= T – c
4π Vc
2 Vc

The density and the period of oscillation T are related as follows:

ρ = A T2 + B (see illustration)

A and B are constants which are determined by the elasticity, structure and mass of the
measurement tube. As the mass varies from tube to tube, it must be determined by
measurement. We refer to this as a factor determination or adjustment of the instrument. The
factor of the measurement tube is calculated by measuring the period of oscillation T of two
standard substances of known density, e.g. air and water:

K ρA – ρW ρA = Density of air [g/cm3]

F= = 2 2 ρW Density of water [g/cm3]
4π2 Vc TA – TW =
TA = Period of oscillation of the air measurement [s]
TW = Period of oscillation of the water measurement [s]
T2
The unknown density of a substance ρS
2 is calculated after the factor
T
W determination by measurement of its
period of oscillation using the following
2
T
S
formula:

F (TA2 – TS2) = ( ρA – ρS)

Solving for ρS gives:

2
T
A
ρ
ρ = 0.00120
A
ρ
S
ρ
W
= 0.99821 ρS = ρA – F (TA2 – TS2)

Note: The factor is temperature dependent. The volume of the measurement tube and its
period of oscillation change with temperature. It is thus necessary to determine the
factor of the cell for the temperature at which you measure your samples!

METTLER TOLEDO DE40/50/51 Density Meter 9

Density

The here described period of oscillation T is used to calculate the tube factor and from that
the density of the unknown sample. But this period of oscillation is not displayed on the
instrument. The DE40/DE50/DE51 display only the so-called T-value which is directly
related to the period of oscillation T:

Period of oscillation T = T-value • Z • 0.2 µsec • (1/600)

with: Z = 10 for DE50/DE51
Z = 100 for DE40
T-value shown as T on the display of the instrument

So the T-value is an auxiliary value that makes use of the maximum display possibilities of
the instrument, using only digits and no decimals. This gives the maximum resolution
especially for the determination of the stability.

The stability for accepting the measured T-value can be set at three different levels (see
Section 8.3) to reduce measurement time, according to the need of the customer:

Stability 0: The stability of the measured signal (T-value) is waited for: ∆Tval ≤ 4 within 77 s
for the DE50/DE51, and ∆Tval ≤ 1 within 49 s for the DE40. The density is
measured with an accuracy of ±1*10-5 (DE40: ±1*10-4).
Stability 1: The final T-value is extrapolated with a patented algorithm from the course of the
slope (∆Tval/∆temp.), and the density is calculated. This cuts the measurement
time about by half while the result (i.e. density) in most cases is identical to that of
stability 0.
Stability 2: The final T-value is extrapolated at an earlier point with a patented algorithm
from the course of the slope (∆Tval/∆temp.), allowing a slightly higher
uncertainty, and the density is calculated. Thus the measurement time is cut
about by half again, while the density for most of the samples lies within ± 1 or 2
digits of that of stability 0.

End value

Time

End of measurement
with: stability 2 stability 1 stability 0

10 METTLER TOLEDO DE40/50/51 Density Meter

 Density

3.3 Construction of the measuring cell and temperature control

For correct density measurements a very accurate temperature control and measurement
must be guaranteed.
As the temperature in the sample can not be measured directly without disturbing the
measurement of the oscillation, a different approach is used. The temperature is measured
in three different spots:

- temperature 1: sensor placed in the measuring cell, directly over the surface of the
measurement tube. This temperature is shown on the status display (cell
temperature).
- temperature 2: temperature of the thermoblock, not displayed (block temperature).
- temperature 3: reference temperature inside the instrument, not displayed (ambient
temperature).

From these three temperatures the actual temperature in the sample is calculated with a
patented algorithm.

Temperature sensor 3 (ambient temperature)

Temperature controller
Thermoblock

Peltier element

Oscillator / Sample outlet

Vibration meter Sample inlet
Thermal insulation

Magnet Temperature sensor 1 Measurement tube

(cell temperature)
Temperature sensor 2
(block temperature)

The cell temperature is compared with the inputted set temperature. The temperature
controller uses this cellblock/set difference to control the thermoelectric module (Peltier
element) which heats or cools the thermostatic block accordingly.

METTLER TOLEDO DE40/50/51 Density Meter 11

The instrument

4. The instrument 16. Connector to dry air

17. Pump in connector
(only withASU-DE)
Front view 7. Sampling speed
1. Desiccator control (only with
ASU-DE)
8. Sampling/Drain switch
2. Measuring cell (only with ASU-DE)

3. Cell window 9. Drain tube

10. Waste bottle

4. LCD display (only with ASU-DE)

11. Sampling tube

5. Keypad

6. Memory card slot

12. GA42 printer (optional)

1. Desiccator
For drying the measuring cell. Filled with silica gel.
2. Measuring cell
Thermostated measuring cell (Oscillating glas U-tube).
3. Cell window
To have a view into the measuring cell (check for air bubbles).
4. LC display
The DE40, DE50 and DE51 have a seven-line liquid crystal display that shows measurement results,
conditions or parameters.
5. Keypad (alphanumeric)
Start measurement, input of measurement conditions or calculation parameters are operated on keys.
6. Memory card slot
To load and download data method and sample files and to load applications.
7. Sampling speed control (ASU-DE)
To set the sampling speed (high for high viscous samples, low for low viscous samples).
8. Sampling/Drain switch
When Sampling position is selected, the speed is controlled by the Sampling speed control. When Drain
position is selected, the speed is set to maximum (not controlled).
9. Drain tube
Connects the measuring cell out to the pump in (ASU-DE) or to a waste ( without ASU-DE).
10. Waste bottle (ASU-DE)
Collects waste liquid (sample and rinsing solutions).
11. Sampling tube (ASU-DE)
Connect to the measuring cell in.
12. GA42 Printer (optional)
METTLER TOLEDO matrix printer.

12 METTLER TOLEDO DE40/50/51 Density Meter

 The instrument

Rear view 19. Waste bottle tube

connection (only with
13. Measuring cell out ASU-DE)
20. Pump exhaust (only
14. Measuring cell in
with ASU-DE)

15. Drain hole 21. Printer

connector
16. Dry air out 22. RS-232C
connector
17. Pump in connector
(only with ASU-DE) 23. Power switch

18. Sample 24. AC power connector

changer connector
25. I/O connector
26. Ext. cell or AD-510
13. Measuring cell out
Measuring cell ouput connector. Connect the drain tube here (9).
14. Measuring cell in
Measuring cell input connector. Connect the sampling tube here (ASU-DE) or a syringe (no ASU-DE).
15. Drain hole
To drain sample or rinsing solutions in case of leakage.
16. Dry air out
To dry the measuring cell connect the sampling tube (11) to this connector which is attached the desiccator (1).
17. Pump in connector (ASU-DE)
Connect the drain tube here (ASU-DE).
18. Connector for sample changer
connection of the SC24, SC24H, SC24L, CHG-260B sample changer, or the DCU-300B auto rinsing unit.
19. Waste bottle tube connection (ASU-DE)
Connect these tubes to the waste bottle lid (black tube to the higher connector: air suction; white tube to the
main lid: sample in).
20. Pump Exhaust
The exhaust air from the waste bottle goes out here.
21. Printer connector
This is for connection to an external printer(GA42 printer).
22. RS-232C connector
This is for connection to a personal computer or to our RE40 and RE50 refractometers.
23. Power switch
The power of DE40/50/51 is turned on or off by this switch.
24. AC power connector
Connect the power cable to the power supply. The instrument will automatically adjust its power supply unit
to the specific line voltage of your country as long as this is in the range between 100 and 240 V AC.
25. I/O connector
For connection of the SOP40 or SOP50.
26. Ext. cell
Connector for an external cell (option) or for the automatic drain unit AD-510 (option).

METTLER TOLEDO DE40/50/51 Density Meter 13

The instrument

4.2. Keyboard

Display Sample

Print Report

Func. Meas.
Para

Setup Calc.
Para

7 8 9 Check Calib.
Rinse/
ABC DEF GHI
BS Purge Pump Method

4 5 6
JKL MNO PQR

1 2 3 Enter
STU VWX YZ

. 0 - Clear Esc. Reset Measure

BLK

Description of each key and its function:

Sample Sample: Key to set up sample parameters, like sample number and ID/No.

Report: Key to change print format.

Report
There are three kinds of format for printing.
(1) Short: Short form for printing minimum necessity of result data.
(2) GLP: GLP form, which complies with GLP requirements.
(3) Variable: User defined printing format.

Meas. Measuring Key to change measuring parameter:

Para
parameter - Method name (8 characters, alphanumerical),
- Measuring temperature (from 15 to 70 °C),
- Stability (0, 1 or 2),
- Limit Time,
- Sample sequence (sampling, drain, rinse, purge and cell test),
- Calibration mode (air & water, other standards)

Calc. Calculation Key to change calculation parameter:

Para
parameter - Result (d, d(t/t), d(t/4), application software). Setup concentration
function.

14 METTLER TOLEDO DE40/50/51 Density Meter

 The instrument

Method Method: Key to select the method (0 to 9). The selection is done either by
pressing the "Up" and "Down" key or by pressing the corresponding
number key.
Display
Display: Key to change display or select numerics, capital or smallleters.

Print Print: Key to print the displayed message or parameters.

Function Function: Key to select following functions:

(0) Data File (statistics, delete data, re-calculate),
(1) Sample File,
(2) Method File,
(3) Changer (DCU-300, SC24, CHG-241),
(4) Factor (adjustment factor),
(5) Set Check (name, ref.value, tolerance),
(6) Periodic Check, Periodic Calibration (interval, show list),
(7) Auto Start (multiple measurement),
(8) CARD utility (install applications),
(9) Memory Clear (meas.data, meas.para, check list, calib. list, etc...).

Setup Setup: Key to select the setup parameters as follows:

(0) Interface: Set up the RS-232C and the printer
(1) Date & Time: Set up date and time
(2) Name: Register name (laboratory or operator)
(3) Serial/Ver. No.: Display serial and version No. of the unit
(4) LCD Contrast: Adjust brightness of LC display
(5) International: Select format for date and select unit (°C/°F) for
temperature
(6) Lock: Set up a password for protection
(7) Beep: Select beep (on / off, duration)
(8) Temp.Calib: Run temperature calibration

Check Check: Key to start the instrument check: daily (always active) or precision
(only when due).

Calib. Calibration: Key to start a calibration.

BS Backspace: Key to erase one preceding letter or numeral.

Rinse/
Purge Rinse/Purge With SC24 sample changers only. Runs an automatic rinse and purge
cycle, according to the selected sample sequence (see Meas.Para).

METTLER TOLEDO DE40/50/51 Density Meter 15

The instrument

Pump Pump For ASU-DE sampling pump. Starts and stops the pump.

Clear Clear: Key to erase the whole entry (2 x to clear names)

Esc.
Escape: Key to return to the main display

Reset
Reset: Key to abort a measurement or a calibration and to return
to main display.
Measure Measure: Key to start a measurement

7 0 – 9 key Key to enter alphanumeric characters (the selection capi

ABC
tal/small letter or number is done with the <Display> key).
To select B, press twice, for C press three times.
On the key with the number 0 there is BLK (BLK = blank)
to insert blank spaces..

. – Dot and hyphen key

Enter Enter Key to confirm key entry. This key is also called [Enter]
key in this manual.

Up

Left, Right
 Keys to move the cursor. The Left and Right keys are also
used for selection within the menus.

Down


16 METTLER TOLEDO DE40/50/51 Density Meter

 The instrument

4.3 Display
The DE40, DE50 and DE51 density meters have a seven line LCD display with 40 characters
per line. When the power is turned on (power switch on the rear of the instrument), the
following message will appear on the display.

5 place Density Meter The software version appears for a

short time. This software version can
DE50 Version X.XX
also be displayed under Setup 3
"Serial/Version".

After a few seconds the main display will appear:

0.Method-0 01-01
Temp.(°C) Oscillation The message "Wait for Reay" will
blink until the cell temperature
Set 20.00
Cell 21.42
845393 reaches the set temperature.

Wait for Ready

Once the cell temperature has reached the set temperature:

0.Method-0 01-01 The "No calibration" message

Temp.(°C) Oscillation
appears if the instrument has not
Set 20.00
Cell 20.00
845393 been calibrated before or if a
Memory Clear has been performed
No Calibration (see section 10.10 "Memory Clear").

Note: The Oscillation value is the T-value described in section 3.2 "Measurement principle".

0.Method-0 01-01 You can toggle between all available

Temp.(°C) Density(g/cm3) units by pressing the <DISPLAY>
key:
Set 20.00
Cell 20.00 0.00120 - Oscillation (T-value)
- Density [d(t/4, d(t/t)] (1)
No Calibration - Concentration (2)
- ALCOHOL (3)
- BRIX (3)

(1) Selection of d, d(t/t: Specific Gravity), d(t/4) is done under Calc.Para – Density.
(2) Selection of Concentration is done under Calc.Para – Result.
(3) Selection of ALCOHOL, BRIX is done under Calc.Para – Result, but requires to install the
application from a special Application Memory Card (see section 13).

METTLER TOLEDO DE40/50/51 Density Meter 17

Getting started

5. Getting started

Before connecting the power cord after unpacking the instrument loosen or remove the three
security screws: remove the backplane in the upper part of the instrument and loosen the
three large screws (marked with red) just right of the pump box (refer to section 15.2 for more
help).
These screws are used as a transportation security only to hold in place the measuring cell
during shipment. For correct operation they must be loosened or removed.

When the instrument is switched on the first time, it is recommended to reset it electronically
to its default values as described in section 10.10 (function 9, memory clear: all parameter).

5.1 Setting up the instrument

5.1.1 Printer (Setup 0)
If you work without printer you can skip this section and go directly to section 5.1.2.
Perform the following steps if you have a GA42 printer to connect:
- Make sure that the DIP switch 2 is ON on the GA42 printer.
- Press the <SETUP> key.

< Setup >

Select "0.Interface". Confirm with
0.Interface 5.International <ENTER>.
1.Date & Time 6.Lock
2.Name 7.Beep
3.Serial/Version 8.Temperature
4.LCD Contrast

< Interface >

Select "1.Printer". Confirm with
0.RS-232C <ENTER>.
1.Printer

< Printer >

Printer : IDP- DP- GA- Other Select "GA-" with the → key.
Exit :[Execute] Confirm with <ENTER>.

- To choose the format of the printed results press the <REPORT> key and choose between
"None", "Short", "GLP" and "Variable" (see section 7 "Report").
- Then press the <RESET> key.

18 METTLER TOLEDO DE40/50/51 Density Meter

 Getting started

5.1.2 Date and time (Setup 1)

- Press the <SETUP> key.
< Setup > Select "1.Date&Time". Confirm with
0.Interface 5.International <ENTER>.
1.Date & Time 6.Lock
2.Name 7.Beep
3.Serial/Version 8.Temperature
4.LCD Contrast
< Date & Time > Actual date and time are displayed.
Press <ENTER> key.
16/07/1998 17:24
Enter date and time (the date format
Day :[16] Hour :[17] is selected in the menu Setup „5.
Month :[ 7] Minutes:[22] International”) and confirm with
Year :[1998] Exit :[Execute] <ENTER>.

5.1.3 Enter the lab name (Setup 2)

- Press the <SETUP> key.
< Setup >
0.Interface 5.International Select „2. Name”. Confirm with
1.Date & Time 6.Lock <ENTER>.
2.Name 7.Beep The capital A means that the
3.Serial/Version 8.Temperature
4.LCD Contrast alphanumerical keyboard is in entry
mode for capital letters. Press the
< Name > A <DISPLAY> key to change the entry
Name : [Quality Lab 1 ] mode:
A: capital letters (A, B, C, D, ..)
Exit : [Execute] a: small letters (a, b, c, d, ...)
1: numbers (0, 1, 2, 3, 4, ...)

Enter the lab name or the instrument name using the alphanumeric keys, e.g. „Quality
Lab 1”, and confirm with <ENTER>. The instrument is now ready for calibration and the first
measurements.

5.2 Performing the first adjustment (calibration)

For correct measurements the instrument should always be adjusted at the measuring
temperature.
After turning on the instrument allow at least 20 minutes for the instrument to warm up
before performing the first adjustment.
Note:
According to the International Vocabulary of Basic and General Terms in Metrology from DIN
the operation of bringing a measuring instrument into a state of performance suitable for its
use is called adjustment.

METTLER TOLEDO DE40/50/51 Density Meter 19

Getting started

The earlier used term calibration should not be used anymore for this purpose as soon as
instrument settings are changed or adapted in order to ensure correct measurement values.
We recommend to select one method which you will dedicate to the adjustment:
Method 9 < Meas.Parameter > VA
Select
Method Name :[ADJUST ] Stability 0
Measurement Temp.:[ 20.00]°C
Stability :[0] and Limit Time 0 s.
Limit Time :[ 0]s
Sequence : Off On

5.2.1 Clean the measuring cell

a) If you have a sampling pump ASU-DE:
- Insert the sampling tube into a beaker containing a high volatile solvent (e.g. acetone or
ethanol).
- Press the <PUMP> key . Flush the measuring cell during approximately 10 s. Adjust the
sampling speed with the Sampling Adj. control at the rear right side of the instrument so that
it takes approximately 5 s from starting the pump to fill the measuring cell (the solvent
appears in the drain tube at the top of the cell). During the pumping it is recommended to
remove the sampling tube from the beaker and insert it again to create some air bubbles.
This will result in a much more efficient cleaning.
- Remove the sampling tube from the beaker.
- Press the <PUMP> key again to stop the pump.
b) If you don't have a sampling pump ASU-DE (only a built-in air pump):
- Flush the measuring cell using a syringe filled with approx. 10 mL of high volatile solvent
(e.g. acetone or ethanol). Suck back the remaining solvant with the syringe.

5.2.2 Dry (purge) the measuring cell

If you have a sampling pump ASU-DE or not, the following steps will be the same:
- Insert the sampling tube into the dry air outlet (white connector without black ring).
- Keep the <PUMP> key pressed for approx. 2 s (until the message "Purge Checking"
appears on the display.
9.ADJUST 01-01 This Purge Checking will auto-
Temp.(°C) Oscillation matically turn off the pump when the
cell will be dry, i.e. when the
Set 20.00
Cell 20.00
845393 oscillation value doesn't change
Purge Checking anymore.
Note: you can also stop the pump
yourself by pressing the
<PUMP> key .

20 METTLER TOLEDO DE40/50/51 Density Meter

 Getting started

5.2.3 Performing the air adjustment

Whether you have a sampling pump ASU-DE or not, the following steps will be the same:
- Press the <CALIB.> and follow the directions on the display:
9.ADJUST 01-01
Temp.(°C) Oscillation If the cell is really clean and dry,
press <ENTER>.
Set 20.00
Cell 20.00 845393
Purge OK?
9.ADJUST 01-01 While adjusting to the air value the
Temp.(°C) Oscillation
display shows:
Set 20.00
Cell 20.00
845393
Calibrating
9.ADJUST 01-01
Temp.(°C) Oscillation Wait until the next message is
displayed.
Set 20.00
Cell 20.00
845393
Set Water
5.2.4 Performing the water adjustment
- Open a vial of certified water (one of the five delivered water standards). This water will be
used for the adjustment.
Note: you can also use bi-distilled water, but we recommend to use our certified water for
traceability.

a) If you have a sampling pump ASU-DE:

- Remove the sampling tube from the measuring cell.
- Remove the drain tube connection from the ASU-DE pump (white connector with black ring).

b) The next steps will be the same wether you have a sampling pump ASU-DE or not:
- Pierce the septum of the certified water standard with the tip of the syringe.
- Fill the syringe with approx. 8 mL of certified water.
- Remove the tip of the syringe and insert the syringe in the sample inlet (lower measuring tube
connection).
- Perform an analytical rinse, i.e. fill approx. 2 mL carefully, wait for 10 s without removing the
syringe, then fill in again 2 mL very carefully. During the filling watch carefully that no air
bubbles enter the measuring cell.

METTLER TOLEDO DE40/50/51 Density Meter 21

Getting started

- Leave the syringe in the sample inlet .

- Press <ENTER>.
9.ADJUST 01-01 While adjusting to the water value
Temp.(°C) Oscillation the display shows:
Set 20.00
Cell 20.00
1086415
Calibrating
9.ADJUST At the end the calibration result is
Temp.(°C) displayed. The number on the right
side is the calibration factor (see
Set 20.00
Cell 20.00
OK 2.141349 section 3.2 "Measuring principle")
and can be considered as a cell
Calibration constant.

[Calibration]
Date : 29/06/1998 11:15

Result : OK The results of the adjustment are

Temperature : 20.0 °C printed on the attached printer:
OLD –––> NEW
Air
T 845392 845393
d 0.00120 0.00120

Water
T 1086413 1086415
d 0.99820 0.99821

Factor
2.141345 2.141349

Name : Quality Lab 1

----------------

9.ADUST
Temp.(°C) When the instrument fails to do the
adjustment it will show the error
Set 20.00
Cell 20.00 FAILED message:

Calibration
In such a case clean the measuring cell well, dry it, and repeat the adjustment. If it fails again,
then exchange the pure water (make sure it really is of the appropriate quality. i.e. our
certified water standard, doubly distilled or correctly deionized). If the adjustment still does
not succeed, contact your dealer for technical assistance.

22 METTLER TOLEDO DE40/50/51 Density Meter

 Getting started

5.3 Perform a measurement

Perform for example two measurements with your standard sample.

5.3.1 Set the parameter of the method

- Press the <MEAS.PARA> key.
Method 0 < Meas.Parameter > VA Enter your method name and
measuring temperature. Leave
Method Name :[TestMeth]
Measurement Temp.:[ 20.00]°C stability and limit time as given.
Stability :[1] Select "Sequence On" with the →
Limit Time :[ 600]s key (with ASU-DE). The sequence
Sequence : Off On
has to be "Off" without ASU-DE.
Method 0 < Meas.Parameter > VA
Sampling : Off Set Auto Setting of the sequence with
Sampling Time :[ 5]s ASU-DE: Set the parameters accor-
Drain : Off Set Auto ding to the following displays.
Drain Time :[ 10]s
Rinse-1 : Off Set
Method 0 < Meas.Parameter > VA
Rinse-1 Time :[ 5]s
Rinse-2 : Off Set Auto
Purge : Off Set Auto
Purge Time :[ 60]s
Cell Test : Off On
Method 0 < Meas.Parameter >
Calib. :<Air&Water>
Exit : [Execute]

5.3.2 Define the sample file (with or without ASU-DE)

- Press <SAMPLE> to enter the sample data: sample number and sample ID.

< Sample > Enter sample no, for example 02-01:

press 2 <ENTER> 1 <ENTER>.
Sample No. : [02]-[01]
Sample ID : [ORANGE ]
Exit : [Execute] Enter your sample ID using the
alphanumeric keys (max. 10
characters) and confirm with
<ENTER>.

METTLER TOLEDO DE40/50/51 Density Meter 23

Getting started

5.3.3 Sampling with ASU-DE

- Dip the sampling tube into the beaker containing your sample.
- Press the <MEASURE> key.
11.TestMeth ORANGE 02-01 The sampling will be done auto-
Temp.(°C) Oscillation matically, according to the
predefined time.
Set 20.00
Cell 20.86
1086414
Sampling Once the sampling is done, remove
the sampling tube from the beaker.

5.3.4 Sampling without ASU-DE

- Fill the syringe with approx. 3 mL of your sample.
- Insert the syringe in the sample inlet (lower measuring tube connection).
- Fill the measuring cell with the syringe (approx. 2 mL). During the filling watch carefully that
no air bubbles enter the measuring cell.
- Leave the syringe in the sample inlet.

5.3.5 Measurement
0.TestMeth ORANGE 2-01 Press the <MEASURE> key.
Temp.(°C) Oscillation
Set 20.00
Cell 20.86
1086414 Press the <DISPLAY> key to switch
Measuring from Oscillation to Density.

0.TestMeth ORANGE 02-01

Temp.(°C) Density(g/cm3)
When the measurement is finished,
Set 20.00
Cell 20.00
0.99820 the result is displayed and printed.

Result *** Result ***

Sample No. 02-01

Date : 29/06/1998 11:15

Note: This printout corresponds to the selection Sample ID : ORANGE
GLP for the report. See section 7 "Report" Method No. : 0
Method Name : TestMeth
for more information. Meas.Temp. : 20.00 °C
Period T : 1086422
d[g/cm3] : 0.99821

Meas.Time : 00:00:36

Name : Quality Lab 1

--------------

24 METTLER TOLEDO DE40/50/51 Density Meter

 Getting started

5.3.6 Cleaning and rinsing of the cell with ASU-DE

0.TestMeth ORANGE 02-01 Follow the instructions on the

Temp.(°C) Density(g/cm3) display for the rinsing and purging.
Use a highly volatile solvent (e.g.
Set 20.00
Cell 20.00
0.99820 acetone or ethanol) for the rinsing.
Set Rinse-1

5.3.7 Cleaning the cell without ASU-DE

- suck back the sample into the syringe.
- flush the measuring cell with appropriate solvent.
- if necessary flush again with a highly volatile solvent (for example acetone or ethanol) to
facilitate the drying of the cell.
- dry the measuring cell according to section 5.2.2.

METTLER TOLEDO DE40/50/51 Density Meter 25

Sample and sample file

6. Sample (Number and ID) and Sample File

Here you can define your sample identifications like sample number and sample ID.

The sample number is composed of two parts: for example [03]–[01].

[03]: The first two-digit number – ranging from 00 to 98 – represents the series number of
your measurement series. In the instrument it is called "High Sample No.". If you group your
measurements of different sample types by this series number, you can conveniently
process your data statistically later on.
[01]: The second two-digit number – ranging from 00 to 99 – is automatically increased by
one per measurement.

Additionally you can define a sample ID, for example a sample name or the Lot number.

Press the <SAMPLE> key.

< Sample > Enter the sample ID using the alpha-

numeric keys (max. 10 characters
Sample No. : [03]-[01] including "space", "period" and
Sample ID : [ORANGE ]
Viscosity : off on (*) "hyphen").
Exit : [Execute] (*) Appears with DE51, or with DE40
with viscosity correction (memory
card).

6.1 Sample File (sample changer)

If you have connected a sample changer (see Function 3 "Changer") or set a sample file (see
Function 1 "Sample File") another display will appear when pressing the <SAMPLE> key:

< Sample > The first line is usually skipped,

Sample File :Skip Load Save except when you want to SAVE OR
Max.Meas.No. :[ 24] load a sample file with a Memory
Next.Meas.No :[ 1]
Method :Current Variable Card (see section 12.8 to 12.10).
Set Parameter :[Execute]
Exit :[Execute]
Max.Meas.No:
This is the total number of samples to be measured. This number can be higher than 24
(when used with the SC24). This number can be changed during the measurement. For
example set this number to 12 if you have only 12 samples to measure when you start and
change it later to 25 if you get 13 more samples to measure.

Next.Meas.No:
This is the next measurement number (according to the sample file). It is increased
automatically after each measurement.

26 METTLER TOLEDO DE40/50/51 Density Meter

 Sample and sample file

Method:
Here you can select if you want to perform all measurements with one method only (current
method) or with different methods (variable).

Example:
You always get a series of 12 samples of grape juice and 7 samples of orange juice to
measure. You want to check for each series your instrument with a check standard.
You will have to set up 3 different methods as follows:

Method-0 Method-1 Method-2

Method Name Grape.J CheckStd Orange.J
Meas.Temp. 20.00°C 20.00°C 20.00°C
Stability 2 0 2

You will the define a sample file as follows:

< Sample >
Sample File :Skip Load Save
Max.Meas.No. :[ 20]
Next.Meas.No :[ 1]
Method :Current Variable
Set Parameter :[Execute]
Exit :[Execute]

< Sample List> This sample file will run all 12 Grape
No. M S.No. Sample ID juices with method 0 "Grape.J",
1 0 01-01 GRAPE
2 0 01-02 GRAPE then the check standard with
3 0 01-03 GRAPE method 1 "CheckStd" (this method
4 0 01-04 GRAPE has a higher stability as it is required
5 0 01-05 GRAPE to check the instrument),
6 0 01-06 GRAPE
7 0 01-07 GRAPE then run all 7 orange juices.
8 0 01-08 GRAPE
9 0 01-09 GRAPE
10 0 01-10 GRAPE
It will allow to run the statistics on
11 0 01-11 GRAPE
each sample separately.
12 0 01-12 GRAPE
13 1 50-01 STANDARD
14 2 02-01 ORANGE
15 2 02-02 ORANGE If the next series would be without
16 2 02-03 ORANGE the first 12 grape juices, you would
17 2 02-04 ORANGE have to select:
18 2 02-05 ORANGE Max.Meas.No: 20
19 2 02-06 ORANGE Next.Meas.No: 13
20 2 02-07 ORANGE

METTLER TOLEDO DE40/50/51 Density Meter 27

Sample and sample file

6.2 Viscosity correction

For viscosity corrected measurements the instrument has to be adjusted with the viscosity
correction set to "On". Viscosity correction is available for the DE51 (standard) or for the
DE40 (optional Memory Card).

Select "Viscosity" to be on in the Sample display as below.

< Sample > (*) This line appears only with the
Sample No. : [03]-[01] DE51, or the DE40 with viscosity
Sample ID : [ORANGE ] correction (Memory Card.)
Viscosity : off on (*)
Exit : [Execute]

When working with a Sample File the viscosity correction can be chosen for each sample
individually.
< Sample List >
No. M S.No.Sample ID Vis.
1 1 01-01 ABC On
2 1 01-02 DEF On
3 1 01-03 GHI On
4 2 01-04 JKL Off
5 2 01-05 MNO Off

The measurement display shows "Visc.", if the sample is measured with viscosity correction.

0.Method-0 01-01 Note: When Viscosity correction

Temp.(°C) Density (g/cm3) is chosen "On", the Stability
of the measurement will be
Set 20.00
Cell 20.00 Visc. 1.23456 fixed to "O".
Measuring
In viscosity correction mode the instrument first waits for stability, which will be signalized
with a beep, and then starts the viscosity correction measurement.
In GLP mode the T-values without and with viscosity correction as well as the corrected
density will be printed.

28 METTLER TOLEDO DE40/50/51 Density Meter

 Report

7. Report (password protected)

In the <REPORT> menu you can choose the format of your printout according to the
following table.

Short GLP Variable

Name X X X
Model, Serial, Version X choice
Sample No. X X choice
Date & Time X X choice
Sample ID X choice
Method No., Method name X choice
Meas.Temp. X X choice
Period T X choice
Meas. Data X choice
Temp. Comp. X choice
Result X X choice
Meas. Time X choice

These report parameters can be set individually for each of the 10 methods.

METTLER TOLEDO DE40/50/51 Density Meter 29

Measuring parameters

8. Measuring parameter (password protected)

This section describes how to set the measuring parameters for the methods. These
measuring parameters can be set for each of the 10 methods:

Parameter Default value

Method name Method-x
Measuring temperature 20.0 °C
Stability (0-2) 1
Limit Time 0s
Sequence Off

< Method List > Press the <METHOD> key.

No. Name Temp.(°C) Result . First select the method for which you
0.Method-0 20.00 d
1.Method-1 20.00 d want to set the measuring
2.Method-2 20.00 d parameters.
3.Method-3 20.00 d Press <ENTER> to confirm.
4.Method-4 20.00 d

8.1 Method name

This option is used to define method names for each method. You can enter names accor-
ding to products or according to operators, depending on your needs.

- Press the <Meas.Para> key.

Method 0 < Meas.Parameter > VA The default name is displayed.
Press the <CLEAR> key twice to
Method Name :[Method-0] clear the name.
Measurement Temp.:[ 20.00]°C
Stability :[1] Press the <DISPLAY> key to select
Limit Time :[ 600]s the input mode of the keyboard:
Sequence : Off On A: CAPITAL LETTERS (A, B, C,...)
a: small letters (a, b, c, d, ...)
1: numbers (0, 1, 2, 3, ...)

Examples of method names (either by product or by operator):

Product names Operator names
Orange Smith
Grape Nelson
Lemon McNeil
Apple Gordon
. .

Enter your method name using the alphanumeric keys (max. 8 characters) and confirm with
<ENTER>.

30 METTLER TOLEDO DE40/50/51 Density Meter

 Measuring parameters

8.2 Measuring temperature

This option is used to set the measuring temperature.

Method 0 < Meas.Parameter > VA Enter your measuring temperature

(from 4 °C to 70 °C, 4 °C to 90 °C
Method Name :[Method-0] for DE40) and confirm with
Measurement Temp.:[ 20.00]°C
Stability :[1] <ENTER>.
Limit Time :[ 600]s
Sequence : Off On

Note: After changing the set temperature make sure to allow enough time for the instru-
ment to warm up.

To increase the temperature: To decrease the temperature:

from to change time from to change time

10 °C ⇒ 20°C +10 °C 30 min 20 °C ⇒ 10°C -10°C 1 hour

20 °C ⇒ 30°C +10 °C 30 min 30 °C ⇒ 20°C -10°C 1 hour

20 °C ⇒ 70°C +50 °C 1 hour 70 °C ⇒ 20°C -50°C 2 hours

8.3 Measurement stability

This option is used to set the measurement stability, which allows to shorten the measuring
time. See section 3.2 "Measurement principle" for more details.
Method 0 < Meas.Parameter > VA
Method Name :[Method-0]
Measurement Temp.:[ 20.00]°C
Stability :[1]
Limit Time :[ 600]s
Sequence : Off On

Enter your stability (0, 1 or 2) and confirm with <ENTER>.

0: Maximum stability. The measured signal (T-value) has to be stable before the density is
accepted. Longest measuring time (Time-0).
1: High stability. The final T-value is extrapolated, and the density is calculated. Medium
measuring time. ( Time-1 is approximately 1/2 of Time-0) .
2: Low stability. The final T-value is extrapolated from less values, and the density is
calculated. Lowest measuring time. ( Time-2 is approximately 1/4 of Time-0).

Note: Stability is fixed to zero when viscosity correction is active (section 6.2).

METTLER TOLEDO DE40/50/51 Density Meter 31

Measuring parameters

8.4 Limit time

This option is used to set the limit time. When this time limit is reached, the measurement is
stopped and the measured value taken, even if it is not stable.

Method 0 < Meas.Parameter > VA Enter the limit time (enter 0 s for no
Method Name :[Method-0] limit time).
Measurement Temp.:[ 20.00]°C
Stability :[1]
Limit Time :[ 600]s
Sequence : Off On

8.5 Sequence (sampling, drain, rinse, purge)

This option is used to set a sample sequence. This sample sequence is mainly used together
with the sampling pump ASU-DE or with the sample changer SC24. It can be used as a SOP
(Standard Operating Procedure) with the ASU-DE. This sample sequence allows you to
define sampling time, drain time, rinsing times (for two different solutions) and purge time.
Method 0 < Meas.Parameter > VA
Select sequence "On" with the →
Method Name :[Method-0] key to set a sample sequence.
Measurement Temp.:[ 20.00]°C
Stability :[1] Confirm with <ENTER>.
Limit Time :[ 600]s
Sequence : Off On

Method 0 < Meas.Parameter > VA Select the input mode of the

sampling time:
Sampling : Off Set Auto Set : Manual input, time is set by
Sampling Time :[ 5]s operator.
Drain : Off Set Auto
Drain Time :[ 10]s Auto: Automatic setting of sampling
Rinse-1 : Off Set time. (see section 8.7)
Off : No sampling.

If you have selected "Set" you have to enter the sampling time (0 to 9999 s). For most of the
non viscous samples we recommend a sampling time of 5 s. Once you have set the sampling
time, adjust the sampling speed (Sampling Adj. control on the ASU-DE or on SC24) in order
to get the sample in with this sample time.
Method 0 < Meas.Parameter > VA
If you have selected "Auto" you have
Sampling : Off Set Auto to enter the "Over Sampling Rate"
O.S.Rate :[ 70]% and the "Sampling Limit".
Sampling Limit :[ 0]s
Drain Time : Off Set Auto
Drain Time : [ 10]s

32 METTLER TOLEDO DE40/50/51 Density Meter

 Measuring parameters

O.S.Rate: The instrument will detect how much time it needs from starting the pump
until the sample reaches the measuring cell and will add the O.S.Rate to
this time.
Sampling Limit:This is the sampling time which should not be exceeded. After this time the
pump automatically stops.

Method 0 < Meas.Parameter > VA Select the input mode of the drain
time:
Sampling : Off Set Auto
O.S.Rate :[ 70]% Set : Manual input, time is set by
Sampling Limit :[ 0]s the operator.
Drain : Off Set Auto Auto: Automatic setting of drain
Drain Time : [ 10]s
time. (see section 8.7).
Off : No draining.

If you have selected "Set" you have to enter the drain time (0 to 9999 s). A typical drain time
for non-viscous samples is about 10 s to 20 s.

Method 0 < Meas.Parameter > VA If you have selected "Auto" you have
to enter the Drain Rate. The
Sampling : Off Set Auto
O.S.Rate :[ 70]% instrument will use this percentage
Sampling Limit :[ 0]s of the sampling time as drain time.
Drain : Off Set Auto
Drain Rate : [ 100]%
Method 0 < Meas.Parameter > VA Select the input mode of the Rinse-1
time:
Rinse-1 : Off Set
Rinse-1 Time :[ 10]s Set : Manual input, time is set by
Rinse-2 : Off Set operator.
Rinse-2 Time :[ 10]s Off : No Rinse-1.
Purge : Off Set Auto

If you have selected "Set" you have to enter the Rinse-1 time (0 to 9999 s). We recommend
to use a dedicated solvent for your sample as Rinse-1 solution.

Method 0 < Meas.Parameter > VA Select the input mode of the Rinse-2
time:
Rinse-1 : Off Set Set : Manual input, time is set by
Rinse-1 Time :[ 10]s
Rinse-2 : Off Set operator.
Rinse-2 Time :[ 10]s Off : No Rinse-2.
Purge : Off Set Auto

METTLER TOLEDO DE40/50/51 Density Meter 33

Measuring parameters

If you have selected "Set" you have to enter the Rinse-2 time (0 to 9999 s). We recommend
to use a volatile solvent as Rinse-2 solution (e.g. acetone). This will allow to speed up the
purging of the measuring cell.

Select the input mode of the Purge time:

Method 0 < Meas.Parameter > VA Set: Manual input, time is set by
the operator.
Rinse-1 :Off Set
Rinse-1 Time :[ 10]s Off: No Purging.
Rinse 2 :Off Set Auto: Purge will stop automatically
Rinse-2 Time :[ 10]s
Purge: :Off Set Auto when the cell is dry, i.e. when
the oscillation value doesn't
change anymore.

If you have selected "Set" you have to enter the Purge time (0 to 9999 s).

If "Set" or "Auto" has been selected for the purge action you can set a "Cell Test" to be
performed after the end of the drying action (purge).

Method 0 < Meas.Parameter > VA Select Cell Test: "On" to perform an

automatic cell test at the end of the
Cell Test :Off On
Reliability :[10] purge sequence.
Calib. :<Air & Water>
Exit :[Execute] Enter the reliability value.

The "Cell Test" measures the density of the empty cell (air) and compares it to the theoretical
density of air at this temperature (value is stored in the instrument). A reliability value can be
entered, which stands for the maximum tolerated difference between the measured and
theoretical density of air. Its default value is 10.

A reliability of 10 means: - DE40: ±10 * 10-4 g/cm3

- DE50 and DE51: ±10 * 10-5 g/cm3

8.6 Adjustment mode (Calibration selection)

This option is used whenever you want to select special standards for adjustment. The
standard adjustment mode is with air & water.

Method 0 < Meas.Parameter > VA Select Calib.:<Other STD> to

perform the adjustment with your
Cell Test :Off On
Calib. :<Other STD> own density standards.
STD-1 Density :[ 0.99821] Enter the values of these two
STD-2 Density :[ 1.49320] density standards.
Exit :[Execute]

34 METTLER TOLEDO DE40/50/51 Density Meter

 Measuring parameters

Note: The Cell Test is not available if you select "Other STD". Only very accurate density
standards should be used for adjustment with other standards, otherwise your
density meter could give wrong results.
8.7 Explanation of auto-sampling and auto-draining
You have selected "Auto" for the sampling and for the draining, for example with:
Over Sampling rate = 30%
Drain rate = 70%

If the time measured from starting the pump until the sample reaches the measuring cell
(with ASU-DE or SC24) is 10 s, then the sampling and the drain time will be:
Sampling time = 10 + (10 x 0.30) = 13 s
Drain time = 13 x 0.70 = 0.91 s

When using this oversampling rate (O.S.Rate) make sure that the measurement cell as well
as the ingoing tubings are empty and dry when you press the <MEASURE> key. The
instrument detects the sharp change of the measurement signal caused by the sample
entering the cell.

METTLER TOLEDO DE40/50/51 Density Meter 35

Calculation parameters

9. Calculation parameters (password protected)

This section describes how to select the type of results (density, specific gravity,
concentration), the number of decimals for these results and how to set the parameters for
the concentration calculation. These calculation parameters can be set for each of the 10
methods.

Press the <CALC.PARA> key.

Method 0 < Calc.Parameter >
0.Density
1.Result
2.Temperature Compensation
3.Decimals

9.1 Density: d, d(t/4), d(t/t)

Select CALC.PARA - Density
Select what density should be
Method 0 < Density >
displayed and printed:
Density :d d(t/4) d(t/t) d: mass density (real density).
Exit :[Execute] d(t/4): relative density referred to
water at 4 °C.
d(t/t): relative density referred to
water at the measurement
temperature.
At 20 °C: 20
d(t/t) = d20 and d(t/4) = d420

9.2 Result (Density, Concentration, BRIX, ALCOHOL, USER TABLE)

Select CALC.PARA - Result.
Select which result should be
displayed and printed:
Method 0 < Result >
d: density, resp. d/t/t) or d(t/4),
Result :<d > depending on the selected
Exit :[Execute] density (previous step).
Conc.: concentration.

Note: here you can also select concentration applications, if they have been previously
loaded with a Memory Card (See also section 13 "Application Memory Cards):
BRIX, ALCOHOL, USER TABLE.

36 METTLER TOLEDO DE40/50/51 Density Meter

 Calculation parameters

Concentration

Method 0 < Result >

Select "Conc." with the → key.
Result :<Conc. > Confirm with <ENTER>.
Exit :[Execute]

You will select this option if you want to determine the concentration of two-component
solutions, e.g. aqueous solutions of glycerol or acetone. Ask your local representative for
available concentration tables or download them from our Internet Homepage:
www.density.com.

There are two ways to program a concentration function, either by entering this function
directly (provided that this function is already known) or by entering a concentration table
(data pairs of density and corresponding concentration).

First you have to select the unit for your concentration. This unit should be the same as that
of the solution used: the instrument does not convert units.

Method 0 < Result > Select your "Conc.Unit" with the →

Result : <Conc. > key. Confirm with <ENTER>.
Conc.Unit : <None > Following units are available: No
Conc.Formula : <A+Bx > unit, %, ppm, mg/g, mg/mL, mg/L,
Parameter Set : Table Coefficient g/L, Eq/L, mol/L, g/cm3.
Data Replace : <x<-d-R >

Then you have to select the concentration formula. If your concentration function is known
already, select the same function. If your concentration function is not known (input of
concentration table) select the formula which best fits your concentration. As a rule of thumb
we recommend to select A+Bx+Cx2 if you don't know which formula to select.

Case 1: Direct input of concentration function (known concentration function)

We will describe this function using an example: measurement of Apparent Extract in beer.
The function to convert density into Apparent Extract is:
20 20 2
Apparent Extract = -460.234 + 662.649 x d20 - 202.414 x [d20 ]

The following steps have to be performed:

Method 0 < Result > Select "%" as Conc.Unit with the →

key. Confirm with <ENTER>.
Result : <Conc. >
Conc.Unit : <% >
Conc.Formula : <A+Bx+Cxx > Select "A+Bx+Cxx" with the → key.
Parameter Set : Table Coefficient
Data Replace : <x<-d-R > Confirm with <ENTER>.

METTLER TOLEDO DE40/50/51 Density Meter 37

Calculation parameters

Method 0 < Result > Select "Coefficient" with the → key.

Confirm with <ENTER>.
Result : <Conc. >
Conc.Unit : <% >
Conc.Formula : <A+Bx+Cx2 > Select "x <- d" with the → key.
Parameter Set : Table Coefficient Confirm with <ENTER>. See also
Data Replace : <x<-d >
paragraph "Constants used" on
page 41.
Method 0 < Result >
A :[ -460.234]x10[ 0] Enter the A, B and C values
B :[ 662.649]x10[ 0] Confirm with <ENTER>.
C :[ -202.414]x10[ 0]
Exit :[Execute]

Now the DE40 (DE50 or DE51) has been programmed to measure APPARENT EXTRACT
in beer. Load the measuring cell with degased beer (any air bubbles would lead to wrong
results). After pressing <MEASURE>, the following values will be displayed:

0.Method-0 01-01 During the measurement the

Temp.(°C) Conc.(%) temperature and the concentration
are displayed.
Set 20.00
Cell 20.00
1.8804
Measuring
At the end of the measurement the
final result will be displayed
0.Method-0 01-01
Temp.(°C) Conc.(%) (APPARENT EXTRACT
concentration).
Set 20.00
Cell 20.00 1.88 Note: you can select the number of
Result decimal places under "Calc.
Para.-Decimals".

Note: This concentration function can also be used to convert the density into other units,
e.g. into API degree. The function used to convert density into API degree is:
20
API degree = [(1/d20 ) - 1] x 141.5 + 10

Case 2: Input of concentration table (concentration function unknown)

We will describe this function using an example: measurement of sulfuric acid concentration
(aqueous solution). You will have to enter a concentration table (data pairs of density and
corresponding concentration), the DE40 (DE50, DE51) will determine automatically the
correct coefficients for the concentration function.

38 METTLER TOLEDO DE40/50/51 Density Meter

 Calculation parameters

Concentration table for sulfuric acid (from "Handbook of Chemistry and Physics").

Concentration d Note: see our Internet Homepage

(in %) (at 20 °C) www.density.com for other concen-
1 10 1.0661 tration tables.
2 20 1.1398
3 30 1.2191
4 40 1.3028
5 50 1.3952
6 60 1.4987
7 70 1.6105
8 80 1.7272
9 90 1.8144

Method 0 < Result > Select "%" with the → key. Confirm
with <ENTER>.
Result : <Conc. >
Conc.Unit : <% >
Conc.Formula : <A+Bx+Cx2 > Select "A+Bx+Cx2" with the → key.
Parameter Set : Table Coefficient Confirm with <ENTER>.
Data Replace : <x<-d-R >

Method 0 < Result > Select "Table" with the → key.

Result : <Conc. > Confirm with <ENTER>. The best
Conc.Unit : <% > results are obtained if <x← d-R> is
Conc.Formula : <A+Bx+Cx2 > selected for data replace (see note
Parameter Set : Table Coefficient on next page).
Data Replace : <x<-d-R >

Method 0 < Result > Enter the number corresponding to

the number of data pairs to be
Data No. (3-30) : [ 9]
Enter Conc.Table: [Execute] entered (e.g. 9 in this example). Up
Exit : [Execute] to 30 data pairs can be entered.

Method 0 < Conc.Table > Select "Edit" with the → key.

No. Conc. Density . Confirm with <ENTER>.
1 [10.0000] [ 1.06610]
2 [20.0000] [ 1.13980]
3 [30.0000] [ 1.21910] Enter the data pairs according to the
4 [40.0000] [ 1.30280] table.
Edit Clear Get Para. Exit

Method 0 < Conc.Table > Enter all other data pairs according
No. Conc. Density . to the table.
6 [60.0000] [ 1.49870]
7 [70.0000] [ 1.61050] Select "Get Parameter" with the →
8 [80.0000] [ 1.72720] key. Confirm with <ENTER>.
9 [90.0000] [ 1.81440]
Edit Clear Get Para. Exit

METTLER TOLEDO DE40/50/51 Density Meter 39

Calculation parameters

< Get Parameter >

[A+Bx+Cx2 ]Conc The results of the calculation are
A: 5.199161x101 shown and can be printed.
B: 1.054394x102
C:-3.253848x101
R: 1.419311 x

Constants used
The A, B and C values are the coefficients of the selected function. The R value is the mean
value of all the entered density values. The instrument replaces the density values with the
density minus mean value (x←d-R) to optimize the algorithm used (compute lower values).

Note: these A, B, C and R values can be used to input the concentration function directly
without having to enter the table again.
In this case R is the mean value of all density values and (x←d-R) should be
selected as "Data Replace" for the function.

Now your density meter has been programmed to measure the concentration of sulfuric acid.
Insert an aqueous solution of sulfuric acid into the measuring cell. After pressing
<MEASURE>, the following values will be displayed:

0.Method-0 01-01 During the measurement the

Temp.(°C) Conc.(%) temperature and the concentration
are displayed.
Set 20.00
Cell 20.00
23.5176
Measuring At the end of the measurement the
final result (sulfuric acid
concentration) will be displayed.
0.Method-0 01-01
Temp.(°C) Conc.(%)
Set 20.00 Note: you can select the number of
Cell 20.00 23.5 decimal places under "Calc.
Result Para.-Decimals".

40 METTLER TOLEDO DE40/50/51 Density Meter

 Calculation parameters

9.3 Temperature compensation

This section describes how to set the temperature compensation. It allows to measure the
sample at one temperature and compensate the result to another temperature. There are
three main applications which use this temperature compensation:
- The sample is to viscous to be introduced into the measuring cell or even solid (e.g. paraffin).
Therefore you need to heat the sample up to get it liquid enough to introduce it into the
measuring cell (e.g. 60 °C) but you want to get the density at 15 °C.

Note: for one special application there is already an Application Memory Card available
which allows to measure OIL products at any temperature and get the density at
15 °C or 60 °F. See section 14.1 "API software" for more information.

- Speed up the measurement. For example, if your sample comes at 40 °C and you have to
get the density at 20 °C you can set the measuring temperature to 40 °C and compensate
the result to 20 °C (provided you know the slope of the density against temperature of your
specific sample).
- The reference temperature is out of the instrument range. For example if you have to get the
density at 100° C (maximum measuring temperature of the DE50 is 70 °C). You can set the
measuring temperature to 70 °C and compensate the result to 100 °C (provided you know
the slope of the density against temperature of your specific sample).

Press the <Calc.Para> key.

Method 0 < Calc.Parameter > Select "3.Temperature Compen-
sation". Confirm with <ENTER>.
0.Density
1.Result
2.Temperature Compensation
3.Decimals

Method 0 < Temp.Comp> Select "On" with the → key. Confirm

with <ENTER>.
Temp.Comp. : Off On
Exit :[Execute]

Method 0 < Temp.Comp> Enter the temperature at which you

want to have the result.
Temp.Comp. : Off On
Comp.Temp. :[ 20.00]°C
Temp.Formula :<A+Bx >
Parameter Set :Table Coefficient
Data No.(2-30) : 20]

METTLER TOLEDO DE40/50/51 Density Meter 41

Calculation parameters

There are two ways to program a temperature compensation function, either by entering this
function directly (provided that this function is already known) or by entering a temperature
compensation table (data pairs of temperature and corresponding density). See previous
section 9.2 Result:

Case 1: Direct input of concentration function For input of temp.comp. function

Case 2: Input of concentration table For input of temp.comp. table

9.4 Decimals
This section describes how to set the decimals for your results (d, concentration).

Press the <Calc.Para> key.

Method 0 < Calc.Parameter > Select "3.Decimals". Confirm with
<ENTER>.
0.Density
1.Result
2.Temperature Compensation
3.Decimals

Method 0 < Decimals > Enter the number of decimals for the
Density (0-5):[5] density (d) respectively specific
Conc. (0-5):[5] gravity: d(t/t), d(t/4).
Brix (0-3):[3]
Alcohol (0-3):[3]
Exit :[Execute]

Enter the number of decimals for the different other options:

Conc: Concentration (must have been previously selected under Calc.Para-Result)

BRIX: Sugar concentration (must have been previously loaded with the Application
Memory Card and selected under Calc.Para-Result)

Alcohol: Ethanol concentration (must have been previously loaded with the Application
Memory Card and selected under Calc.Para-Result)

Note: The measured values will be displayed and printed out according to the selected
settings.

42 METTLER TOLEDO DE40/50/51 Density Meter

 Functions

10. Functions (password protected)

10.1 Data File (Function 0)

This function allows to view and process the measured data. You can either do a
recalculation of measured results, statistics on the results or delete single data i.e. mark
them so that they are not considered in the statistics calculations. You can also save these
measured data on a Data Memory Card or load them from such a card for recalculation.
Select Function "0.Data File" and
< Function >
confirm with <ENTER>.
0.Data File 5.Set Check
1.Sample File 6.Periodic
2.Method File 7.Auto Start The measured data are displayed,
3.Changer 8.CARD utility together with the method and result
4.Factor 9.Memory Clear type:
- d : density
< Data File > - dt : specific gravity (d(t/t)
No. M.Name Sample Result .
0 Method-0 01-01 d 0.99820 - d4 : specific gravity (d(t/4)
1 Method-0 01-02 d 0.99819 - C : concentration
2 Method-1 02-01 Br 10.81 - Br : BRIX
3 Method-1 02-02 Br 10.82
Statis. Recalc. Load Save Exit - Al : ALCOHOL

Now select with the ← and → keys between <Statistics>, <Recalc.>, <Load> and <Save>
and confirm with <ENTER>.

Note 1: If you have many data stored, press 0 to display the first 4 data (0 to 3), and 1 to 9
to display the next blocks of data (10 to 13, 20 to 23, ... 90 to 93).

Note 2: The <Load> and <Save> options require a Data Memory Card. These options are
described separately in section 12."Data Memory Cards".

10.1.1 Statistics
The function allows to process and view the measured data statistically. You can calculate
the mean value (Mean), the standard deviation (SD) and the relative standard deviation
(RSD). The relative standard deviation is the same as the coefficient of variation (CV).

The measured data can be filtered so that useful statistical results can be obtained after a
days work. On the first level the method will give access to the respective data only. On the
second level the series number (first two-digit number of the sample number) can be chosen
for which the statistics is to be done.

Note: If too many filters are activated it is possible that all results are excluded.

METTLER TOLEDO DE40/50/51 Density Meter 43

Functions

< Data File > After selecting <Statis.> and con-

No. M.Name Sample Result . firmation with <ENTER>, you can
0 Method-0 01-01 d 0.99820 select the filter to process only the
1 Method-0 01-02 d 0.99819
2 Method-1 02-01 Br 10.81 desired results: result, method no,
3 Method-1 02-02 Br 10.82 method name, high sample no.
Statis. Recalc. Load Save Exit Select the desired filter to be "On"
using the → key.
< Statistics >
Result :<Brix >
Method No. :<Off>
Method Name :<Off> Now you select whether the
High Sample No. :<Off> statistics results are to be printed
Data Print :<Off > alone (Data Print "Off") or together
Statistics :[Execute] with a list of all the measured values
< Statistics Result > (Data Print "On").

Results : 4 The statistics is now shown on the

Mean : 10.82 %
SD : 0.06 % display.
RSD : 0.54 %
Exit :[Execute]

<Statistics> To print the results of the statistics

Date : 29/06/1998 17:29 calculation press the <PRINT> key.
Sample No.(High) : 01
Method No. : 2
Select Exit : [Execute] and confirm
Results: 4 with <ENTER> to exit.
Mean : 10.82 %
SD : 0.06 %
RSD : 0.54 %

Name : Quality Lab 1

----------------

10.1.2 Recalculation
This function allows to recalculate single measured values. Before you do this you have to
change the calculation formula (or calculation table) in the respective method to the desired
one (like density to BRIX, shown in this example). When you now enter the recalculation
menu the result will be shown directly according to the new calculation setting.

< Data File > Select the data to be recalculated

No. M.Name Sample Result . with the <UP> and <DOWN> keys.
11 Method-5 01-01 d 1.03981
12 Method-5 01-02 d 1.03980 Select Recalc. with the ← and →
13 Method-6 02-01 Br 10.81 keys and confirm with <ENTER>.
14 Method-6 02-02 Br 10.82
Statis. Recalc. Load Save Exit

44 METTLER TOLEDO DE40/50/51 Density Meter

 Functions

0.Method-5 01-02 Press the <CALC.PARA> key.

Temp.(°C) Density(g/cm3)
Set 20.00
Cell 20.00
1.03980
Recalculation
Recalc. < Calc.Parameter > Select "1.Result" and confirm with
0.Density <ENTER>
1.Result
2.Temperature Compensation
3.Decimals

Recalc. < Result > Select Brix with the → key and
Result :<Brix > confirm with <ENTER>
Exit :[Execute]

Select Function 0.Data File again

< Data File > The recalculated result is shown
No. M.Name Sample Result . (from density to BRIX)
11 Method-5 01-01 d 1.03981
12 Method-5 01-02 Br 10.40
13 Method-6 02-01 Br 10.81
14 Method-6 02-02 Br 10.82
Statis. Recalc. Load Save Exit

10.1.3 Data Deletion

This function allows to exclude single measured values from the statistics calculation by
marking them with an asterisk (they are not deleted from the list).

< Data File > Select the data to be excluded with

No. M.Name Sample Result . the <UP> and <DOWN> keys.
1 Method-0 01-01 d 10.81
2 Method-0 01-02 Br 8.40
3 Method-0 01-03 Br 10.81 Exclude this data by pressing the
4 Method-0 01-04 Br 10.82 <DISPLAY> key.
Statis. Recalc. Load Save Exit

< Data File > The excluded data is marked with an

No. M.Name Sample Result . asterisk.
1 Method-0 01-01 d 10.81
* 2 Method-0 01-02 Br 8.40
3 Method-0 01-03 Br 10.81
4 Method-0 01-04 Br 10.82
Statis. Recalc. Load Save Exit

METTLER TOLEDO DE40/50/51 Density Meter 45

Functions

Now you can resume the statistics calculation again and then print the results without the
marked measurements. If you selected the data print "On" the excluded measurements will
be printed with an asterisk before the sample number.

10.2 Sample File (Function 1)

This function is used normally when the instrument is operated in connection with a sample
changer.
< Sample File > It is automatically set to ON when a
Sample Changer has been selected
Sample File : Off On (see Function 3).
Exit : [Execute]

It can also be used without Sample Changer to run standard measurements always in the
same sequence. See also section 6.1 "Sample File" for more information.

10.3 Method File (Function 2)

This function is used together with a Data Memory Card to save and load a method file.
< Method File >
No. Name Temp.(°C) Result .
0. ORANGE 20.0 d See section 12. "Data Memory
1. GRAPE 20.0 d Cards" for more information.
2. LEMON 20.0 d
3. APPLE 20.0 d
Load Save Exit

10.4 Changer (Function 3)

Here you can define the connected sample changer (SC24 or CHG-241) or auto sampling
unit DCU-300.

Press function 3 (Changer) and confirm with <ENTER>.

< Changer > Choose the desired equipment using

Changer : <SC24 > the → key:
Exit : [Execute] None, DCU-300, SC24, SOP40,
SOP50 or CHG-241. Confirm with
<ENTER>.

When choosing the SC24 you can define whether the measuring series is started with a
calibration (On) or not (Off).
Note: Select CHG-241 if you connect a CHG-260.

46 METTLER TOLEDO DE40/50/51 Density Meter

 Functions

< Changer > Selecting Calib. "On" means that in

the starting position there has to be
Changer : <SC24 > a vial with deionized water, and that
Calib. : Off On
Start Position : Home Current sample vials are placed after
Exit : [Execute] this position. When pressing
<MEASURE> the instrument will
first perform a calibration with air
and water. Only after this will the
sample measurement be done.

< Changer > Here you can define the starting

position on the turntable. You can
Changer : <SC24 > select that measurements are
Calib. : Off On
Start Position : Home Current started at "Home" (number 1 on the
Exit : [Execute] turntable) or "Current" (the actual
position on the turntable). Confirm
with <ENTER>.

10.5 Factor (Function 4)

If you know the period of oscillation T and the density of air and water for a particular
temperature, you can enter these values here manually for the calculation of the factor.

< Factor >

Temp. :[ 20.00]°C
Air T/d :[ 845401]/[ 0.00120]
Water T/d :[ 1086417]/[ 0.99821]
Correct :[Execute]
Factor :[ 2.141349]
Exit :[Execute]

10.6 Set check (Function 5)

In this function you can set up define the substances - name, rated value and allowed
tolerances - you want to use as standard check samples either for the daily or the periodic
check.

Select function 5 - Set Check - and confirm with <ENTER>.

< Set Check >
With the ← and → keys select either
Check :Daily Periodic "Daily" or "Periodic" and confirm with
Standard :[Fl. Carbon ] <ENTER>.
Ref.Value :[ 1.94796]g/cm3
Tolerance :[ 0.00004]g/cm3
Exit :[Execute]

METTLER TOLEDO DE40/50/51 Density Meter 47

Functions

10.6.1 Daily Check

This check is used mainly to control the working condition of the instrument. Is the instrument
really clean and dry? Did the previous operator really clean the cell after measuring his sample?

Pressing the Check key will always activate a daily check, unless a periodic check is due (see
also section 10.7).

Normally deionized or bi-distilled water is used for this purpose. Of course any other solution
can be used that suites the purpose i.e. is readily and in abundance available as well as has
a stable and well known composition.
< Set Check > This example uses water as daily
check solution.
Check :Daily Periodic The results of this daily check are
Standard :[Water ]
Ref.Value :[ 0.99821]g/cm3 not stored in the instrument. They
Tolerance :[ 0.00008]g/cm3 are just meant to check whether the
Exit :[Execute] instrument is really ready for the
next measurement.

10.6.2 Periodic Check

This check is used mainly to verify whether a new adjustment of the instrument is necessary
or not. Every adjustment of the instrument means that the instrument settings are changed.
This can be dangerous if it is not done correctly because all the measurements afterwards
will be wrong.
Pressing the check key will start a periodic check only when such a test is due according to
the settings in section 10.7.1. When no periodic check is due it will start a daily check.

So instead of actually performing frequent adjustments we suggest to perform frequent

checks with an exactly known standard solution. As long as this periodic check is done
successfully, there is no need to adjust the instrument. The results of periodic check are read
automatically in the instrument. Should the periodic check fail, the operator should carefully
clean and dry the measuring cell and then repeat the periodic check. If it still fails, then the
instrument should be adjusted again: press the <Calib.> key and follow the instructions of the
instrument.

If the periodic check fails too often even after re-adjusting the instrument - like every second
or third time - then you should contact your local supplier for servicing and closer support.

< Set Check > This example uses toluene as

periodic check solution.
Check :Daily Periodic
Standard :[Toluene ] The results of this periodic check are
Ref.Value :[ 0.86683]g/cm3 stored in the instrument. They can be
Tolerance :[ 0.00002]g/cm3 retrieved under Function "6. Periodic"
Exit :[Execute]
and "Show Check List".

48 METTLER TOLEDO DE40/50/51 Density Meter

 Functions

We recommend to use our certified density standards for a periodic instrument check:
ME-51338002 Water 0.99... g/cm3 +/- 0.00001
ME-51338003 Beverage syrup 1.04... g/cm3 +/- 0.00010
ME-51338004 Dichlorotoluene 1.26... g/cm3 +/- 0.00002
ME-51338005 Fluorocarbon 1.95... g/cm3 +/- 0.00002

10.7 Periodic (Function 6)

This function is used to set the intervals of the periodic adjustment (calibration) as well as the
periodic check. This function is also used to display and print the last adjustment data and
check data stored in the instrument.

10.7.1 Periodic Check

Select function 6 "Periodic" and confirm with <ENTER>.

< Periodic> The display will show the due date

Periodic :Check Calib. for the next periodic check. This due
Next Check Date :19/07)1998
Check Alarm :Off On date is calculated automatically by
Check Interval :[ 7]day the instrument according to following
Show Check List :[Execute] settings. Confirm with <ENTER>.
Exit :[Execute]

< Periodic> Select the periodic check (Check

Periodic :Check Calib. Alarm) to be "On" or "Off", and
Next Check Date :19/07)1998 confirm with <ENTER>.
Check Alarm :Off On
Check Interval :[ 7]day
Show Check List :[Execute]
Exit :[Execute]

If "On" is selected, the instrument will give one of the following messages when it is turned
on: "X Days To Check" as a remainder, "Check Day" on the due date, and "Check Day Over!"
on the following days. Each of these displays will return to the "Ready" display after a while.
If "Off" is selected, the periodic check is not active i.e. whenever you press check, a daily
check is performed.
While running the instrument will not display a message, but pressing the Check key will start
a periodic check.

< Periodic> Enter the check interval (in days)

Periodic :Check Calib. and confirm with <ENTER>
Next Check Date :19/07)1998
Check Alarm :Off On Note: Enter 0 Day to force a periodic
Check Interval :[ 7]day check on today's date. This will
Show Check List :[Execute] especially be required if the periodic
Exit :[Execute]
check has failed, in order to perform
it again.

METTLER TOLEDO DE40/50/51 Density Meter 49

Functions

< Periodic> Select Show Check List Execute to

Periodic :Check Calib. display the list of the last 10 checks
Next Calib.Date :19/07)1998
Check Alarm :Off On performed
Check Interval :[ 7]day The whole list can be printed
Show Check List :[Execute] pressing the <PRINT> key.
Exit :[Execute]

< Show Check List > The results of such a periodic check
No. Date Ref.Value Meas. . are automatically stored in the
10 13/07 0.86683 0.86686 NG instrument. You can view the last 10
9 6/07 0.86683 0.86685 OK
8 29/06 0.86683 0.86684 OK checks performed.
7 22/06 0.86683 0.86684 OK Select the data you are interested in
6 15/06 0.86683 0.86682 OK
and press <ENTER> to get the full
< Show Check List > data dispayed.
Date :13/07/1998
Standard :TOLUENE
Temp. : 20.00 °C
Ref.Value : 0.86683 (+- 0.00002)
Meas.Data : 0.88686 (NG)
Exit :[Execute]

10.7.2 Periodic Adjustment (Calibration)

The instrument has to be adjusted from time to time. You can either choose the time
according to your special needs i.e. perform it when the periodic check fails for example two
times successively, or you can define a certain time period after which the instrument will
remind you to repeat the adjustment.

The results of these adjustments will automatically be stored by the instrument. You can view
at any time the last 10 adjustments performed and also print out the list for documentation
purposes as described below.

Select function 6 "Periodic Calib." and confirm with <ENTER>.

< Periodic> The display will show the due date

Periodic :Check Calib. for the next periodic adjustment.
Next Calib.Date :19/07)1998 This due date is calculated
Calib.Alarm :Off On
Calib.Interval :[ 7]day automatically by the instrument
Show Calib.List :[Execute] according to following settings.
Exit :[Execute] Confirm with <ENTER>.

< Periodic> Select the periodic adjustment

Periodic :Check Calib. (Calib. Alarm) to be "On" or "Off",
Next Calib.Date :19/07)1998
Calib.Alarm :Off On and confirm with <ENTER>.
Calib.Interval :[ 7]day
Show Calib.List :[Execute]
Exit :[Execute]

50 METTLER TOLEDO DE40/50/51 Density Meter

 Functions

If "On" is selected, the instrument will give one of the following messages when it is turned on:
"X Days to Calib" as a remainder, "Calib. Day" on the due date, and "Calib. Day Over!" on the
following days. Each of these displays will return to the "Ready" display after a while.
If "Off" is selected, the instrument will not remaind you to readjust.

< Periodic> Enter the adjustment interval (in

Periodic :Check Calib. days) and confirm with <ENTER>.
Next Check Date :19/07)1998
Calib. Alarm :Off On
Calib.Interval :[ 7]day Note: Enter 0 Day to force a periodic
Show Calib.List :[Execute] adjustment on today's date.
Exit :[Execute]
< Periodic>
Periodic :Check Calib. Select Show Calib.List Execute to
Next Calib.Date :19/07)1998 display the list of the last 10
Calib.Alarm :Off On adjustments performed.
Calib.Interval :[ 7]day
Show Calib.List :[Execute]
Exit :[Execute]
< Show Calib.List > The results of such a periodic
No. Date Temp.(°C) Factor . adjustment are automatically stored
10 13/07 20.00 2.142349 OK in the instrument. You can view the
9 6/07 20.00 2.142342 OK
8 29/06 20.00 2.142337 OK last 10 adjustments performed.
7 22/06 20.00 2.142338 OK Select the data you are interested in
6 15/06 20.00 2.142335 OK and press <ENTER>.
< Show Calib.List >
Date :13/07/1998 By pressing the print key the whole
Temp. : 20.00 °C
Air :T= 845401 d= 0.00120 list is printed.
Water :T= 1086417 d= 0.99821
Factor : 2.142349 (OK)
Exit :[Execute]

10.8 Auto Start (Function 7)

This function is used mainly for repetitive measurements in one sample. It can not be turned
on when there is a connection to a sample changer or to a DCU.

< Auto Start > Press <Function> 7 and confirm with

<ENTER>.
Auto Start :Off On
Exit :[Execute]
Select "On" or "Off" and confirm with
<ENTER>.

METTLER TOLEDO DE40/50/51 Density Meter 51

Functions

< Auto Start > When you have chosen "On", you
can define the time interval between
Auto Start :Off On two measurements. Confirm with
Interval Time :[ 10]min
Meas.Times :[ 0] <ENTER>. Enter 0 to start the next
Exit :[Execute] measurement imme-diately after the
end of the actual measurement (no
time interval).

In "Meas. Times" you define the number of repetitive measurements to be performed with
this sample. Enter 0 for continuous measurement. In this case press Reset to stop the
measurements.

10.9 CARD Utility (Function 8)

This function is used together with the Memory Cards.
< CARD Utility >
0.Data Delete 5.Table Capacity
1.Sample Delete 6.Install Table
2.Method Delete 7.Uninstall Table
3.Format
4.CARD Information

See section 12 "Data Memory Cards" and section 13 "Application Memory Cards" for more
information.

52 METTLER TOLEDO DE40/50/51 Density Meter

 Functions

10.10 Memory Clear (Function 9)

This function is used to reset all parameters to default (factory setting), for instance to
initialize the memory after a serious malfunction of the instrument.
You can also selectively reset single records like the adjustment data (Calib. data), the check
records, the sample files or the method parameters.

Press <Function> 9 and confirm with

< Memory Clear >
<ENTER>.
0.Measured Data 4.Method Parameter
1.Sample File 5.All Parameter
2.Periodic Check Select the desired option:
3.Periodic Calib. "Measured Data", "Sample File",
"Periodic Check", "Periodic Calib.",
"Method Para.", "All Parameter", and
< Measured Data Clear > confirm your choice with <ENTER>.

Clear Execute ? No Yes Select "Yes" to confirm your choice

again and press <ENTER>.

The chosen data or parameters are now deleted and the respective factory settings are
active again.

Note: The function "5. All Parameter" doesn't delete any downloaded special application
tables (from flash memory cards).

METTLER TOLEDO DE40/50/51 Density Meter 53

Setup

11. Setup (password protected)

11.1 Interface (Setup 0)

This option is used to define the RS-232C serial interface and the serial printer.

Press the <SETUP> key.

Select "0.Interface". Confirm with
< Setup >
<ENTER>.
0.Interface 5.International
1.Date & Time 6.Lock
2.Name 7.Beep
3.Serial/Version 8.Temperature
4.LCD Contrast

< Interface > Select "Printer" with the → key in

order to setup the printer or
0.RS-232C "RS-232C" to define the serial
1.Printer
interface.

11.1.1 RS-232C Interface setup

This option is used to setup the RS-232C interface either for connecting a computer or for
connecting a RE40 or RE50 refractometer for simultaneous measurement of refractive index
and density (or specific gravity). In both cases you have to make sure that the other
equipment (computer or RExx) is setup with the same values.
< RS-232C >
Baud Rate :<4800>
Parity :Even Odd None
Stop Bits :1 2
Data Bits :7 8
Exit :[Execute]

Select the following values according to your computer setup or to your RE40 resp. RE50
setup. In case you connect a RExx refractometer, use "Setup 0: Interface" at the
refractometer to make sure that both setups are identical.

54 METTLER TOLEDO DE40/50/51 Density Meter

 Setup

11.1.2 Printer setup

We recommend to select the GA42 printer from METTLER TOLEDO. Make sure that the DIP
switch 2 is ON on the GA42 printer.
< Printer >
Printer :IDP- DP- GA- Other
Exit :[Execute]

The following printers are predefined:

GA-: for GA42 and SPRINTER printer from METTLER TOLEDO.
Other: for all serial printers. Selection of:
- Baud rate (300, 600, 1200, 2400,....)
- Parity (none, even, odd)
- Stop bits (1, 2)
- Data bits (7, 8)
These parameters have to be set according to the specifications of
your printer.
The bold settings are active for the GA42 printer.
IDP-: not used
DP-: not used

11.2 Date and Time (Setup 1)

This option is used to enter the actual date and time.

Press the <SETUP> key.

< Setup > Select "1.Date&Time". Confirm with
<ENTER>.
0.Interface 5.International
1.Date & Time 6.Lock
2.Name 7.Beep
3.Serial/Version 8.Temperature
4.LCD Contrast

< Date & Time > Actual date and time are displayed.
Press <ENTER> key.
12/07/1998 17:12
Enter date and time (the date format
Day :[12] Hour :[17] is selected in the menu Setup
Month :[ 7] Minutes :[12] „5.International”) and confirm with
Year :[1998] Exit :[Execute]
<ENTER>.

METTLER TOLEDO DE40/50/51 Density Meter 55

Setup

11.3 Name (Setup 2)

This option is used to enter the laboratory name (or company or operator).

Press the <SETUP> key.

< Setup > Select "2.Name". Confirm with
<ENTER>.
0.Interface 5.International
1.Date & Time 6.Lock
2.Name 7.Beep
3.Serial/Version 8.Temperature
4.LCD Contrast

< Name > A The capital A means that the

alphanumerical keyboard is in entry
Name :[Quality Lab 1 ] mode for capital letters. Press the
Exit :[Execute] <DISPLAY> key to change the entry
mode:
A: capital letters (A, B, C, D, ..)
a: small letters (a, b, c, d, ...)
1: numbers (0, 1, 2, 3, 4, ...)

11.4 Serial/Version number (Setup 3)

This option is used to identify the software version and the serial number of your instrument
without having to switch off the instrument.

- Press the <SETUP> key.

< Setup > Select "3.Serial/Version".
0.Interface 5.International
1.Date & Time 6.Lock
2.Name 7.Beep
3.Serial/Version 8.Temperature
4.LCD Contrast

< Serial No./Version No.> Press the <ENTER> key. The serial
Serial No. : xxxxxxxx number of your instrument as well
Version No. : x.xx as the software version are
displayed.
[Exit]

56 METTLER TOLEDO DE40/50/51 Density Meter

 Setup

11.5 LCD Contrast (Setup 4)

This option is used to adjust the contrast of the LCD display in your instrument.

Press the <SETUP> key.

< Setup > Select "4.LCD Contrast".
Confirm with <ENTER>.
0.Interface 5.International
1.Date & Time 6.Lock
2.Name 7.Beep
3.Serial/Version 8.Temperature
4.LCD Contrast

< LCD Contrast > Select the LCD contrast with the ←
and → keys. Confirm with
Light Center Dark <ENTER>.
<| | | | | | | | | | |>

11.6 International (Setup 5)

This option is used to select the date format and the unit for the temperature.

Press the <SETUP> key.

< Setup > Select "5.International".
Confirm with <ENTER>.
0.Interface 5.International
1.Date & Time 6.Lock
2.Name 7.Beep
3.Serial/Version 8.Temperature
4.LCD Contrast

< International > Select your date format with the →

key: YYYY: Year
Date :<DD/MM/YYYY> MM : Month
Temp.Unit :°C °F
Exit :[Execute] DD : Day
Select your temperature unit with the
→ key: °C : degree Celsius
°F : degree Fahrenheit

METTLER TOLEDO DE40/50/51 Density Meter 57

Setup

11.7 Lock with password (Setup 6)

Press the <SETUP> key.

< Setup > Select "6.Lock".
Confirm with <ENTER>.
0.Interface 5.International
1.Date & Time 6.Lock
2.Name 7.Beep Select "On" with the → key.
3.Serial/Version 8.Temperature Confirm with <ENTER>.
4.LCD Contrast

< Password Lock > Enter your password. Make sure to

keep it in a safe place in case you
Password Lock :Off On would forget it.
Password :[********]
Confirm Password :[********] Enter your password a second time
Lock Execute :[Execute] to confirm the correct spelling.

In case you would have forgotten your password you will need to call the METTLER
TOLEDO service.

11.8 Beep (Setup 7)

Press the <SETUP> key.
< Setup > Select "7.Beep".
Confirm with <ENTER>.
0.Interface 5.International
1.Date & Time 6.Lock
2.Name 7.Beep
3.Serial/Version 8.Temperature Select "On" with the → key.
4.LCD Contrast Confirm with <ENTER>.

< Beep > Enter your beep selection:

Type1: long and fast
Beep :Off On
Beep Type :<Type1> Type2: long and slow
Exit :[Execute] Type3: short and fast
Type4: short and slow

58 METTLER TOLEDO DE40/50/51 Density Meter

 Setup

11.9 Temperature calibration (Setup 8)

Press the <SETUP> key.
< Setup > Select "8.Temperature".
Confirm with <ENTER>.
0.Interface 5.International
1.Date & Time 6.Lock
2.Name 7.Beep
3.Serial/Version 8.Temperature
4.LCD Contrast

< Temperature > Select either "1.Adjust" (single point)

or "0.Calibration" (multiple point over
0.Calibration the hole temperature range).
1.Adjust Confirm with <ENTER>.

Adjust:
Performs a one-point adjustment at the measuring temperature (correcting the offset).
Select this option when the difference between Set Temperature and Cell Temperature is
less than 0.5 °C (in equilibrium).

Calibration:
Performs a multiple point adjustment, in steps of 5 °C, over the entire temperature range.
Select this option when the difference between Set Temperature and Cell Temperature is
0.5 °C or higher (in equilibrium). This temperature calibration takes approximately two hours!
This temperature calibration will adjust the internal thermistors to the certified thermistor in
the measuring cell over the whole temperature range.

METTLER TOLEDO DE40/50/51 Density Meter 59

Data Memory Card

12. Memory Cards (DE40, DE50 and DE51)

The following two sections describe the use of the memory cards. There are two types of
memory cards to be used with the DE40, DE50 and DE51 density meters:

Data Memory Card: SRAM cards.

For measurement data, methods and sample files (ME-51324004).

Application Memory Card: FLASH cards.

For the following applications:
• ALCOHOL (ME-51324000)
• BRIX (ME-51324001)
• API (ME-51324002)
• VISCOSITY CORRECTION (ME-51324003)
• CONCENTRATION TABLES (ask your local representative)

12.1 Data Memory Cards: data, methods, sample files

These Data Memory Cards are SRAM cards, have a capacity of 1 MB and use a lithium
battery. The cards are used to store measurement data, methods or/and sample files. You
can store up to:
982 Methods (if only methods are stored),
498 Sample files (if only sample files are stored),
10 Data files, each file containing up to 100 data (if only data files are stored).

If you store a combination of methods, sample files and data files the number of available
free space will decrease. In order to see how much free space is available, perform following
steps:

Press the <FUNCTION> key.

< Function > Select “8.CARD utility”. Confirm with
<ENTER>
0.Data File 5.Set Check
1.Sample File 6.Periodic Insert a Data Memory Card into the
2.Method File 7.Auto Start slot on the front left part of the
3.Changer 8.CARD utility instrument.
4.Factor 9.Memory Clear

< CARD utility > Select “4.CARD Information”.

Confirm with <ENTER>.
0.Data delete 5.Table Capacity
1.Sample delete 6.Install Table
2.Method delete 7.Uninstall Table
3.Format
4.CARD Information

60 METTLER TOLEDO DE40/50/51 Density Meter

 Data Memory Card

< Card Information >

Type Used Unused.
Method File : [ 0] [ 982]
Sample File : [ 0] [ 498]
Meas.Data File : [ 0] [ 10]
[Exit]

12.2 Save measured data

With the Data Memory Card you can save data on the card or load data from the card. The
DE40, DE50 and DE51 store up to 100 measurement data in the internal memory. These
data can be saved on a memory card as one single data file. Let’s assume that you have
already performed 35 measurements.

Press the <FUNC> key.

< Function > Select “0.Data File”. Confirm with
<ENTER>.
0.Data File 5.Set Check
1.Sample File 6.Periodic
2.Method File 7.Auto Start
3.Changer 8.CARD utility
4.Factor 9.Memory Clear

< Data File >

No. M.Name Sample Result .
32 ORANGE 01-10 d 1.0326
33 GRAPE 02-01 d 1.0024
34 GRAPE 02-02 d 1.0025
35 GRAPE 02-03 d 1.0024
Statis. Recalc. Load Save Exit

Note: If you have many data stored, press 0 to display the first 4 data (0 to 3), and 1 to 9 to
display the next blocks of data (10 to 13, 20 to 23, ... 90 to 93).

Insert a Data Memory Card into the slot on the front left part of the instrument.
Select “Save” with the → key. Confirm with <ENTER>.

Enter the name under which you

< Save Data File > want to store the measurement data
(max. 8 characters).
File Name : [WEEK24 ]
Save Execute : [Execute] Note: Blank spaces are not allowed.
Exit : [Execute]
Press <ENTER> twice (confirm
Save Execute and Exit).

METTLER TOLEDO DE40/50/51 Density Meter 61

Data Memory Card

< Save Data File >

Wait until all data have been saved.
Now Saving ...

Press <ENTER>.
File save is complete
[ OK ]

Your data are now saved on the memory card, you can clear the internal memory of the
instrument (see next section).

12.3 Clear measured data

Let’s assume that you have saved the previously performed 35 measurements (previous
section). Once your data are saved on the memory card you can clear the internal memory of
the instrument.

Press the <FUNC> key.

< Function > Select “9.Memory Clear”. Confirm

with <ENTER>.
0.Data File 5.Set Check
1.Sample File 6.Periodic
2.Method File 7.Auto Start
3.Changer 8.CARD utility
4.Factor 9.Memory Clear

< Memory Clear > Select “0.Measured Data”. Confirm

with <ENTER>.
0.Measured Data 4.Method Parameter
1.Sample File 5.All Parameter
2.Periodic Check
3.Periodic Calib.

< Memory Data Clear > Select “Yes” with the → key. Confirm
with <ENTER>.
Clear Execute ? No Yes A beep sound will indicate that the
memory has been cleared.

62 METTLER TOLEDO DE40/50/51 Density Meter

 Data Memory Card

12.4 Load measured data

This may be used for example to recalculate some results you have already stored on a
Memory Card. Press the <FUNC> key.
< Function > Select “0.Data File”. Confirm with
0.Data File 5.Set Check <ENTER>.
1.Sample File 6.Periodic
2.Method File 7.Auto Start
3.Changer 8.CARD utility
4.Factor 9.Memory Clear

< Data File > Insert the Data Memory Card with
No. M.Name Sample Result the previously stored data into the
slot on the front left part of the
Data not exist. instrument.
Select “Load”. Confirm with
Exit Load
<ENTER>.
< Load Data File >
File Name Date Time Select the Data File you want to load
WEEK24 06/12/1998 18:02 with the <UP> and <DOWN> keys.
WEEK23 06/05/1998 18:14 Confirm with <ENTER>.
WEEK22 05/29/1998 17:25
WEEK21 05/22/1998 17:45
WEEK20 05/15/1998 18:25

< Load Data File >

Select “Yes” with the → key.
File will be loaded. Confirm with <ENTER>.
OK ?
No Yes

< Load Data File > Wait until the data file has been
loaded.
Now Loading ...

File load is complete Press <ENTER>.

[ OK ]

METTLER TOLEDO DE40/50/51 Density Meter 63

Data Memory Card

< Function >

You can now display the loaded
0.Data File 5.Set Check
1.Sample File 6.Periodic data. Press the <FUNC> key.
2.Method File 7.Auto Start
3.Changer 8.CARD utility
4.Factor 9.Memory Clear

< Data File >

No. M.Name Sample Result .
32 ORANGE 01-10 d 1.0326 Select “0.Data File”. Confirm with
33 GRAPE 02-01 d 1.0024 <ENTER>.
34 GRAPE 02-02 d 1.0025
35 GRAPE 02-03 d 1.0024
Statis. Recalc. Load Save Exit

Note: if you have many data stored, press 0 to display the first 4 data (0 to 3), and 1 to 9 to
display the next blocks of data (10 to 13, 20 to 23, ... 90 to 93).

12.5 Save method

With the Data Memory Card you can save methods on the card or load methods from the
card. The DE40, DE50 and DE51 store up to 10 methods in the internal memory. These
methods can be saved on a memory card and transferred to another instrument. This is
especially useful to develop a method in a central laboratory and once this method has been
validated to distribute it to all other laboratories.
< Function > Press the <FUNC> key.
0.Data File 5.Set Check
1.Sample File 6.Periodic
2.Method File 7.Auto Start Select “2.Method File”. Confirm with
3.Changer 8.CARD utility <ENTER>.
4.Factor 9.Memory Clear

< Method File > Select the method you want to save
No. Name Temp.(°C) Result .
0. ORANGE 20.0 d with the <UP> and <DOWN> keys.
1. GRAPE 20.0 d Select “Save” with the → key.
2. LEMON 20.0 d
3. APPLE 20.0 d Confirm with <ENTER>.
Load Save Exit

< Save Method File > Enter the name under which you
File Name : [GRAPE ] want to store the method (max. 8
Save Execute : [Execute] characters).
Exit : [Execute]
Press <ENTER> twice (confirm
Save Execute and Exit).

64 METTLER TOLEDO DE40/50/51 Density Meter

 Data Memory Card

< Save Method File >

Wait until method has been saved.
Now Saving ...

Press <ENTER>.
File save is complete
[ OK ]

12.6 Clear internal method

Let’s assume that you have saved the previously defined methods (previous section). Once
your methods are saved on the memory card you can clear the internal memory of the
instrument.
< Function >
Press the <FUNC> key.
0.Data File 5.Set Check
1.Sample File 6.Periodic
2.Method File 7.Auto Start
3.Changer 8.CARD utility Select “9.Memory Clear”. Confirm
4.Factor 9.Memory Clear with <ENTER>.

< Memory Clear >

0.Measured Data 4.Method Parameter Select “4.Method Parameter”.
1.Sample File 5.All Parameter Confirm with <ENTER>.
2.Periodic Check
3.Periodic Calib.

< Method Parameter Clear > Select “Yes” with the → key.
Confirm with <ENTER>.
Clear Execute ? No Yes A beep sound will indicate that the
memory has been cleared.

METTLER TOLEDO DE40/50/51 Density Meter 65

Data Memory Card

12.7 Load method

< Function > Press the <FUNC> key.

0.Data File 5.Set Check

1.Sample File 6.Periodic
2.Method File 7.Auto Start Select “2.Method File”. Confirm with
3.Changer 8.CARD utility <ENTER>.
4.Factor 9.Memory Clear
First you have to select where you
< Method File >
No. Name Temp.(°C) Result . want to load the method. Select the
0. Method-0 20.0 d method you want to overwrite with
1. Method-1 20.0 d the one to be loaded with the <UP>
2. Method-2 20.0 d
3. Method-3 20.0 d and <DOWN> keys.
Load Save Exit Select “Load” with the → key.
Confirm with <ENTER>.
< Load Method File >
File Name Date Time .
GRAPE 06/12/1998 18:02
LEMON 06/05/1998 18:14 Select the method you want to load
APPLE 05/29/1998 17:25 with the <UP> and <DOWN> keys.
ORANGE 05/22/1998 17:45
SPRITE 05/15/1998 18:25 Confirm with <ENTER>.

< Load Method File > Select “Yes” with the → key and
confirm with <ENTER>.
File will be loaded.
OK ?
No Yes

< Load Method File > Wait until the method has been
loaded.
Now Loading ...

Press <ENTER>.
File load is complete
[ OK ]

66 METTLER TOLEDO DE40/50/51 Density Meter

 Data Memory Card

< Method File > Repeat the previous steps if you

No. Name Temp.(°C) Result . want to load more methods, being
0. Method-0 20.0 d careful not to overwrite an already
1. ORANGE 20.0 d
2. Method-2 20.0 d defined method: select a free
3. Method-3 20.0 d method “Method-x” to be
Load Save Exit overwritten.
< Method File >
No. Name Temp.(°C) Result . Select “Exit” with the → key. Confirm
0. Method-0 20.0 d with <ENTER>.
1. ORANGE 20.0 d
2. Method-2 20.0 d
3. Method-3 20.0 d
Load Save Exit

12.8 Save sample file

With the Data Memory Card you can save sample files to the card or load sample files from
the card. The DE40, DE50 and DE51 store up to 498 sample files in the internal memory.
These sample files are usually used together with the sample changer, in order to run
samples according to a predefined sequence (sample names, method to be used, etc...).
They can also be used as a SOP (Standard Operating Procedure) if no sample changer is
connected. See section 6.1 for the description of sample files. Let’s assume that you have
already defined such a sample file.

< Function > Press the <FUNC> key.

0.Data File 5.Set Check Select “1.Sample File”. Confirm with

1.Sample File 6.Periodic
2.Method File 7.Auto Start <ENTER>.
3.Changer 8.CARD utility
4.Factor 9.Memory Clear
Select “On” with the → key. Confirm
< Sample File >
with <ENTER>.
Sample File : Off On
Exit : [Execute] Press the <SAMPLE> key.

< Sample > Select “Save” with the → key.

Sample File :Skip Load Save
Max.Meas.No. :[ 24] Confirm with <ENTER>.
Next Meas.No. :[ 1]
Method :Current Variable
Set Parameter :[Execute]
Exit :[Execute]

METTLER TOLEDO DE40/50/51 Density Meter 67

Data Memory Card

< Save Sample File > Enter the name under which you
want to store the sample file (max. 8
File Name : [COKE24 ] characters).
Save Execute : [Execute]
Exit : [Execute]

Press <ENTER> twice (confirm

< Save Sample File > Save Execute and Exit).

Now Saving ...

Wait until:

Press <ENTER>.
File save is complete
[ OK ]

12.9 Clear internal sample file

Let’s assume that you have saved the previously defined sample file (previous section).
Once your sample file is saved on the memory card you can clear the internal memory of the
instrument.
Press the <FUNC> key.
< Function >
0.Data File 5.Set Check
1.Sample File 6.Periodic Select “9.Memory Clear”. Confirm
2.Method File 7.Auto Start with <ENTER>.
3.Changer 8.CARD utility
4.Factor 9.Memory Clear

< Memory Clear >

0.Measured Data 4.Method Parameter Select “1.Sample File”. Confirm with
1.Sample File 5.All Parameter <ENTER>.
2.Periodic Check
3.Periodic Calib.

Select “Yes” with the −> key.

< Sample File Clear > Confirm with <ENTER>.
A beep sound will indicate that the
Clear Execute ? No Yes memory has been cleared.

68 METTLER TOLEDO DE40/50/51 Density Meter

 Data Memory Card

12.10 Load sample file

< Sample > Press the <SAMPLE> key.
Sample File :Skip Load Save
Max.Meas.No. :[ 24]
Next Meas.No. :[ 1] Select “Load” with the → key.
Method :Current Variable Confirm with <ENTER>.
Set Parameter :[Execute]
Exit :[Execute]

< Load Sample File > Select the sample file you want to
File Name Date Time . load with the <UP> and <DOWN>
COKE24 06/12/1998 18:02 keys. Confirm with <ENTER>.
COKE10 06/05/1998 18:14
SPRITE24 05/29/1998 17:25
SPRITE10 05/22/1998 17:45
LIGHT24 05/15/1998 18:25

< Load Sample File > Select “Yes” with the → key.
File will be loaded. Confirm with <ENTER>.

OK ?
No Yes

< Load Sample File > Wait until the sample file has been
loaded.
Now Loading ...

Press <ENTER>.
File load is complete
[ OK ]

METTLER TOLEDO DE40/50/51 Density Meter 69

Application Memory Card

13. Application Memory Cards: BRIX, ALCOHOL, API, VISCOSITY

CORRECTION, USER TABLES

These Application Memory Cards are FLASH cards with a capacity of 2 MB. The cards are
used to download special applications to your instrument. Once the application has been
downloaded to the instrument it is removed from the Application Memory Card. In other
words the Memory Card is empty, but can still be used to retrieve the application from the
instrument, for example to transfer it to another instrument. Therefore make sure to keep the
empty Application Memory Card in a safe place.

Note: The function "Memory Clear" "All Parameter" (in Function 9.5) doesn’t clear the
downloaded application.

13.1 How to download an application (Install table)

This section will show you how to download an application. Following Application Memory
Cards are available:
• ALCOHOL Alcohol concentration (Ethanol) (ME-51324000)
• BRIX Sugar concentration (Sucrose) (ME-51324001)
• API Petroleum products (ME-51324002)
• VISCOSITY Viscosity correction (ME-51324003)
• CONCENTRATION TABLES (ask your local representative)
Insert the Application Memory Card into the slot on the front left part of the instrument.
Press the <FUNC> key.
< CARD utility > Select “8.CARD utility”. Confirm with
<ENTER>.
0.Data delete 5.Table Capacity
1.Sample delete 6.Install Table
2.Method delete 7.Uninstall Table
3.Format
4.CARD Information
< Function > Select “6.Install Table”. Confirm with
<ENTER>.
0.Data File 5.Set Check
1.Sample File 6.Periodic
2.Method File 7.Auto Start
3.Changer 8.CARD utility
4.Factor 9.Memory Clear

Note: The following shows the BRIX application. For other applications just replace BRIX
with your application (Alcohol, API, Viscosity correction, Concentration).

< Install Table > Select the application to load with

Table Name Type Temp. Data. the <UP> and <DOWN> keys.
Brix Conc. 20.00°C 85 Select “Install” with the −> key.
Confirm with <ENTER>.
Exit Install

70 METTLER TOLEDO DE40/50/51 Density Meter

 Application Memory Card

< Install Table > Wait until the installation has been
done.
Now Install ...

Press <ENTER>.
Table install is complete

[ OK ]
The asterisk (*) on the left of the
application (BRIX) shows that this
< Install Table > application has been downloaded
Table Name Type Temp. Data. and removed from the Memory
*Brix Conc. 20.00°C 85 Card. This means that the Memory
Card is empty, but can still be used
Exit Install to retrieve the application from the
instrument (uninstall table).

13.2 How to select the application

The application has been downloaded to the instrument. You need now to define which
method should be dedicated to this application.

Press <METHOD>.
< Method List >
No. Name Temp.(°C) Result . Select the method you want to
0. Method-0 20.0 d dedicate to this application with the
1. Method-1 20.0 d <UP> and <DOWN> keys. Confirm
2. Method-2 20.0 d with <ENTER>.
3. Method-3 20.0 d
4. Method-4 20.0 d Press the <MEAS.PARA> key.

Method 1 <Meas.Parameter>
Set the measurement parameters
Method Name :[ORANGE] according to your application (see
Measurement Temp.:[ 20.00]°C section 8).
Stability (0-2) :[1]
Limit Time :[ 600]s
Sequence :Off On

There are now three different selections, depending on the application:

1. Select concentration table: ALCOHOL, BRIX, CONCENTRATIONS.
2. Select temperature table: API.
3. Select viscosity correction: see section 6.2.

METTLER TOLEDO DE40/50/51 Density Meter 71

Application Memory Card

13.2.1 Select a concentration table: ALCOHOL, BRIX, CONCENTRATIONS

Method 1 <Calc.Parameter> Press the <CALC.PARA> key.

0.Density
1.Result Select “1.Result”. Confirm with
2.Temperature Compensation
3.Decimals <ENTER>.

Method 1 <Result> Select “Brix” (Alcohol,

Concentration, according to your
Result :<Brix >
Exit :[Execute] application). Confirm with
<ENTER>.

Press <ENTER> (confirm Execute).

Press <METHOD>.
< Method List >
No. Name Temp.(°C) Result . The method list now should show
0. Method-0 20.0 d
1. ORANGE 20.0 Brix you the selected method with the
2. Method-2 20.0 d corresponding application.
3. Method-3 20.0 d
4. Method-4 20.0 d

13.2.2 Select a temperature table: API

Method 1 <Calc.Parameter> Press the <CALC.PARA> key.

0.Density
1.Result Select “2.Temperature
2.Temperature Compensation Compensation”. Confirm with
3.Decimals <ENTER>.

Method 1 <Temp.Comp.> Select “Oil Table-1” (Oil Table-2 or

Oil Table-3, according to your
Temp.Comp : Off On
Comp.Temp. :[20.00]°C application) with the → key. See
Temp.Formula :<Oil Table-1> also section 14.1. Confirm with
<ENTER>.

Press <RESET>.

72 METTLER TOLEDO DE40/50/51 Density Meter

 Application Memory Card

13.3 How to retrieve an application (Uninstall table)

This section will show you how to retrieve an application from the instrument, for example to
transfer it to another instrument.
Insert the empty Application Memory Card into the slot on the front left part of the instrument.
Press the <FUNC> key.

< Function > Select “8.CARD utility”. Confirm with

<ENTER>.
0.Data File 5.Set Check
1.Sample File 6.Periodic
2.Method File 7.Auto Start
3.Changer 8.CARD utility
4.Factor 9.Memory Clear

< CARD utility > Select “7.Uninstall Table”. Confirm

with <ENTER>.
0.Data delete 5.Table Capacity
1.Sample delete 6.Install Table
2.Method delete 7.Uninstall Table
3.Format
4.CARD Information
Select “0.Conc.Table” if your appli-
< Uninstall Table >
cation is a concentration table:
0.Conc.Table BRIX, ALCOHOL, CONCENTRATION,
1.Temp.Table or select “1.Temp.Table” if your
2.Exit
application is a temperature table:
API.
Confirm with <ENTER>.

The asterisk (*) on the left of the application (BRIX) shows that this application has been
downloaded and removed from the Memory Card. This means that the Memory Card is
empty, but can still be used to retrieve the application from the instrument.

Select the application to uninstall

with the <UP> and <DOWN> keys.
< Uninstall Table >
Table Name Type Temp. Data. Select “Uninstall” with the -> key.
*Brix Conc. 20.00°C 85 Confirm with <ENTER>.

Exit Uninstall

< Uninstall Table > Wait until the application has been
Now Uninstall ... uninstalled.

METTLER TOLEDO DE40/50/51 Density Meter 73

Application Memory Card

Press <ENTER>.

Table uninstall is complete

[ OK ]

Select “2.Exit” and confirm with

<ENTER>.
< Uninstall Table >
The table on the Memory Card is no
0.Conc.Table longer marked by an asterisk (*).
1.Temp.Table
2.Exit

74 METTLER TOLEDO DE40/50/51 Density Meter

 Special application cards

14. Description of the special applications:

BRIX, ALCOHOL, API, VISCOSITY CORRECTION, COKE, USER
TABLES

The standard software of the DE40/50/51 can be expanded by various software options
(METTLER TOLEDO Service) that are available on Memory Card. This allows you to match
the selection of the method parameters to your requirements.

14.1 API software

The density of crude oils, oil products or lubricants must in some cases be measured at
elevated temperatures owing to their viscosity. With this software, stored tabulated values
are used for the automatic conversion of this density to that at 15 °C or 60 °F, depending on
the temperature unit you measure with. The tabulated values are based on API standard
2540 (API: American Petroleum Institute).
Under the parameters of the temperature compensation, select the required oil table (see
Section 9.2 and 14.4).

Oil table 1: the density of crude oils is converted to the reference temperature of 15° C
(or 60° F) according to table 53A of the API standard.
Oil table 2: the density of oil products is converted to the reference temperature of
15° C (or 60° F) according to table 53B of the API standard.
Oil table 3: the density of lubricants is converted to the reference temperature of
15° C (or 60° F) according to table 53D of the API standard.

To calculate the "API degrees", enter "((1/d) – 1) * 141.5 + 10" as concentration function (see
Section 9.2 and 14.6).

14.2 Alcohol software

With this software the measured density of an alcoholic solution can be converted to the
corresponding volume concentration of Ethanol in water i.e. % v/v. Two different conversion
tables exist for this conversion, depending on the physical unit the temperature is measured
(and the reference temperature):

14.2.1 Temperature measured in °F, with a reference temperature at 60 °F:

This option is activated by selecting the temperature unit °F (see Section 11.6). The stored
values are based on table 3 of NBS Circular 19 (NBS: National Bureau of Standards, since
1988: National Institute of Standards and Technology). You must perform your
measurements at 60 °F to obtain the correct ethanol content.

METTLER TOLEDO DE40/50/51 Density Meter 75

Special application cards

14.2.2 Temperature measured in °C, with a reference temperature at 20 °C:

This option is activated by selecting the temperature unit °C (see Section 11.6). The stored
values are based on table IVa of OIML Guideline No. 22 (OIML: Organisation Internationale
de Métrologie Légale). You must perform your measurements at 20 °C to obtain the correct
ethanol content.
Under the parameters of the concentration calculation, select "Alcohol [%]" instead of a
calculation function (see also Section 9.2). The density and the alcohol concentration are
printed out as the result, e.g.:
Accuracy Reproducibility
DE40: Alcoh 42.1 % ± 0.1 % ± 0.1 %
DE50/51: Alcoh 42.09 % ± 0.01 % ± 0.01 %

Note: The alcohol values of measurements with, e.g. whisky, are relative values: They do
not specify the actual concentration as the density also depends on all other
dissolved substances of this beverage.

14.3 BRIX software

With the BRIX software the measured density of a sucrose/water solution can be converted
to the weight percent sucrose content (°BRIX). The stored tabulated values are based on
table 109 of NBS Circular 440 (NBS: National Bureau of Standards, since 1988: National
Institute of Standards and Technology). The measurement temperature for the automatic
conversion is 20 °C.
Under the parameters of the concentration calculation, select "BRIX [%]" instead of a
calculation function (see also Section 9.2). The density and the BRIX degrees are printed out
as the result, e.g.:
Accuracy Reproducibility (within 0 - 40 %)
DE40: BRIX 10.23 % ± 0.03 % ± 0.03 %
DE50/51: BRIX 10.234 % ± 0.003 % ± 0.003 %

Note: The BRIX values with, e.g. milk are relative values: They do not specify the actual
sucrose concentration as the density also depends on all other dissolved
substances of this beverage.

14.4 Viscosity correction

When measuring samples with higher viscosities (over 10 mPs), the oscillation tube
technique shows a slightly higher value than the real density of the samples. This application
corrects the systematic viscosity error when measuring samples with higher viscosities. See
section 6.2. for more information.

76 METTLER TOLEDO DE40/50/51 Density Meter

 Special application cards

14.5 Coke (Cola drinks)

This Memory Card allows to measure BRIX and HFCS42 (also called SOLIDS) of all Cola
products, from beverages to the highly viscous syrup (concentrate). This card includes
viscosity correction, required for syrup measurement and specially tuned up for Cola
products. This viscosity correction is especially required for products over 40°BRIX (resp.
50°SOLIDS).

14.6 Coefficients for conversion to various concentration data

With all software you can convert the measured density directly to Baumé, Twadell, API or
milk degrees:

In the Calc.Para menu select <Result>, <Conc.>, <Parameter Set>, <Coefficient>. Then:
t t
– select the relative density d 4 or d t
– select the term for the x-axis under Data Replace,
– select A + Bx as calculation function,
– enter the coefficients A and B under Manu Set.

The “Conc” value is printed out as the result in each case.

Parameter Entry
Meas. Coefficients of the
Concentration Conversion temperature Relative Term of the function A + Bx
function [°C] density x-axis
A B

t
Heavy Baumé deg.1) ((1/d) – 1) * -144.3 15.0 d4 (1/d) – 1 0 -144.3
t
Light Baumé deg.1) ((1/d) – 1) * 144.3 + 10 15.0 d4 (1/d) – 1 10 144.3
t
Jap. Sake degrees ((1/d) – 1) * 1443 15.0 d4 (1/d) – 1 0 1443
t
API degrees ((1/d) – 1) * 141.5 + 10 15.56 2) dt (1/d) – 1 10 141.5
t
Twadell degrees (d – 1) * 200 -- d4 d–1 0 200
t
Milk degrees (d – 1) * 1000 -- d4 d–1 0 1000

1)
Note: replace 144.3 by 145 for USA.
2)
15.56 °C corresponds to 60 °F.

METTLER TOLEDO DE40/50/51 Density Meter 77

Automatic sampling pump

15. Automatic sampling pump ASU-DE (optional)

This optional automatic sampling unit replaces the air pump that comes with the basic
installation. With its membrane pump it allows to aspire samples of low to medium viscosity
(at the max. ca. 1000 mPa·s) directly into the measurement tube, to empty, rinse and dry it
after the measurement.
The sequence described in section 8.5, used to control this routine, allows to eliminate
operator specific influences on the measurement which usually occur when using a syringe.
This option allows you to get reproducible results, regardless of the operator using the
instrument.
This pump is also required together with the DCU-300B auto rinsing unit, the SOP40 and
SOP50 and the CHG-260B sample changer.
Instead of using the standard waste bottle of the ASU-DE you can also use the automatic
draining unit AD-510.

15.1 Safety measures

Measures for your safety
- Make sure to switch off the DE40, DE50 or DE51 and disconnect the power cable before
installing the automatic sampling pump ASU-DE!
- Make sure that the solvents used to rinse the measurement tube do not form dangerous
mixtures or reactions when combined with the sample solutions or other solvents, since all
solutions are dejected into the same waste bottle!
- Connect the exhaust tube to a system capable of disposing of poisonous or corrosive gases!

Measures for operational safety

- Empty the waste bottle (drain pot) before it is filled to the rim! Otherwise the waste solution
is sucked into the pump and ejected through the exhaust. This could even damage the pump.
- The material in contact with the liquid for sampling is made of glass, Teflon or carbon. Do
not use any sample that may corrode the material.
Samples of high viscosity cannot be aspirated. Maximum viscosity that can be pumped is
approximately 1000 mPa-s.

78 METTLER TOLEDO DE40/50/51 Density Meter

 Automatic sampling pump

15.2 Installation
- Switch off the DE40/DE50/DE51 and
disconnect the power cable. rail 1
- Place the unit backside down as shown to
attach two rails for the holder of the waste
bottle.
Fasten Rail 1 by two screws. Slide in the
board into rail 1 and 2, and then fasten
rail 2 by two screws. rail 2
- The board can be slid under the instrument if
it is not in use.

- Detach rear panel and plate. Undo the four

screws fastened to the rear panel and pull
the small plate out of the panel as shown
(sits very tight).
- Remove the three large screws (marked with
red) just right of the air pump unit. These
screws are used as transportation security to
hold the measuring cell, which is mounted on
shock absorbers, firmly against the chassis
during transportation. Make sure to keep
these screws for future need of re-shipping.

- Remove the air pump.

a) Undo three screws fastened to the
pump unit.
b) Disconnect the pump connector (1) and
2
dry air tube (2).
c) Move the pump unit first to the right (ca.
3 cm), then up and remove it.
1

Note: Disconnect the dry air tube carefully in

order to avoid damage.

METTLER TOLEDO DE40/50/51 Density Meter 79

Automatic sampling pump

- Install the ASU-DE in the place of the air

pump.
5
a) Slide in the ASU-DE down and to the left,
adjusting its bottom fitting to the angle
hole of the main unit.

b) Fasten the ASU-DE by the three screws

which were used to hold the air pump.

c) Connect the pump connector (3 black,

2-poles) and solenoid connector (4 white, 3
3 poles) to the main unit.
4
d) Connect the dry tube (5) to the joint for
the desicator tower.
- Install rear panel (Follow reverse steps from above).
- Attach the supplied seal (Align to line ”A”).

- Connect the air tube (tygon, with Teflon

connector at one and PP connector at the
other) to the dry air out (without black
tightening clamp).

- Insert the sampling tube (one connector only)

into the measuring cell inlet (lower port),
connect the drain tube (two connectors) to
the measuring cell out (upper port) to the
pump-in connector (black clamp).

Note: Make sure the white Teflon tube

nozzles are firmly tightened clockwise.

80 METTLER TOLEDO DE40/50/51 Density Meter

 Automatic sampling pump

Warning:
If using samples and rinsing solutions which
could create poisonous or corrosive gases,
connect the exhaust tube to the exhaust
connector and lead the other end to a system
capable of disposing of these gases.

- Connecting the waste bottle:

Connect the black tube from the pump unit to
the higher connector of the bottle cap and
tighten the screw firmly. Push the transparent
tube from the pump unit into the ohter
connector of the bottle cap, so that it
protrudes ca. 30 mm below. Then tighten the
screw firmly.

Note: If the connectors are not screwed in tightly, the pump will not (or hardly) be able to
move the sample.

- Pull out the plate for the waste bottle on the left side of the instrument, place the white plastic
mug on it, and put the waste bottle in the mug.

METTLER TOLEDO DE40/50/51 Density Meter 81

Error messages and malfunctions

16. Error Messages and Malfunctions

16.1 Error Messages

Error message Cause Measure

Nozzle error The sampling nozzle did not Remove the obstacle.
move normaly (SC24, DCU-300 or Replace the nozzle if it is bent or
SOP50. borken.
Press [Stop] key to reset the
error.

Table error The rotation of the turnable was Remove the obstacle.
not normal (SC24). Press [Stop] key to reset the
error.

Temperature The set temperature can not be Switch off the instrument and call
control error reached within 2 hours after start. METTLER TOLEDO service.

Communication Cable not connected correctly. Check connection of the

error communication cable.
Wrong instrument selected in Check that the correct instrument
Function 3. is selected in "Function 3": SC24,
CHG, DCU.

Viscosity Correction No adjustment with viscosity correction. Perform adjustment with viscosity cor-
Error rection on.
Air bubbles or smudge in the cell. Check measuring cell.

16.2 Malfunction

Malfunction Cause Measure

Instrument can Cable not plugged in properly, Check power cable and mains.
not be switched fuse blown. Replace fuse if blown (T3.15A/250V).

No "Ready" Air inlet at rear of the instrument Remove obstacles that block the
message is blocked. air inlet.
reached. Cooling fan not operating. Call METTLER TOLEDO service
for help.

Stored data are The internal battery is discharged. Swich off the instrument and call
lost (e.g. time) METTLER TOLEDO service.

Printer not DIP switch of printer not set Check DIP switch setting of the
printing. correctly. printer.
Wrong communication setting for Check setings in "Setup 0".
printer.

82 METTLER TOLEDO DE40/50/51 Density Meter

 Error messages and malfunctions

Malfunction Cause Measure

Nothing displayed Contrast has been changed. Call METTLER TOLEDO service
for help.

No key entry Call METTLER TOLEDO service

possible for help.

Measurement Sample not homogeneous, air bubbles Check factors mentioned.

not reproducible in the measuring tube, measurement Check if temperature is stabilized.
temperature unstable. Measurement cell filled completely?
Is the bench vibrating? Place instrument on a non-vibra-
ting bench.
Cell contaminated with old sample. Clean measuring cell thoroughly.

Air bubbles in the Leaking connections? Check connections.

sample i.e. mea- Sample likely to produce bubbles Degass sample (ultrasonic bath or
surement never or mixture containing components heating) or measure at reduced
ends or measuring with low coiling point? temperature.
times vary greatly. High sample viscosity? Use a syringe or warm up the sample.

Long sampling Wrong setting of the flow control Adjust the sampling speed to your
time. valve. sample with the valve.
Sample viscosity too high. Use a syringe to inject the sample.

Measurement of Switch off the instrument and call the

distilled water free METTLER TOLEDO service.
of air bubbles
takes too long.

Adjustment of the Air or gas bubbles are generated by Boil the distilled water, or degass it in
instrument with air the heating-up of the water in the an ultrasonic bath (at least at the
and water at higher measuring cell. measuring temperature), and inject it
temperatures takes at this higher temperature to avoid
too long. formation of air or gas bubbles.

METTLER TOLEDO DE40/50/51 Density Meter 83

Maintenance

17. Maintenance

17.1 Changing internal battery

The battery is used for data storage and has a service life of around 5 years when it is kept
charged. We thus recommend switching the instrument on now and then to ensure the
battery remains operational during this time.

17.2 Power line voltage, power line, fuse

The DE40/50/51 will adapt automatically to the power line voltage in the range of 100 to
240V and 50/60 Hz.

Changing blown fuses:

- Switch off the instrument and disconnect all cables on the rear of the instrument.
- Put the instrument on the rear side.
- Remove the small plate at the instrument’s bottom plate by unscrewing the 2 screws.
- Change the blown fuse(s) and put the small plate in place again. Make sure to use the correct
fuse type for replacement: T3.15A / 250V.

17.3 Replacing the silicagel

- Replace the silicagel of the drying tube when it becomes pink. If this is not done the drying
of the measurement tube (and thus the adjustment with dry air) is no longer assured.

17.4 Replacing the tube nozzles

If you find air bubbles in the measurement tube on every sample injection which do not
originate from the sample itself, the tube nozzle may be leaking or damaged. To change the
tube nozzles, proceed as follows:
- Carefully remove the protective silicone cap that holds the nozzles correctly in place. Make
sure not to tear at the rim, but hold its strong middle part with your fingers.
- Unscrew the tube nozzle anticlockwise and then screw in the new one tightly by hand. Do
not apply pressure as this would damage the measurement tube.
- Then put the protective silicone cap in place again with the squared hole at the bottom so
that leaking liquid from the cell unit can flow out.

84 METTLER TOLEDO DE40/50/51 Density Meter

 Standard and optional equipment

18. Standard and optional equipment

18.1 Standard equipment

Order No.
Operating instructions Englisch 51709838

Sampling/Drain tubes, 2 pcs (without joint) 51328032

Joint for Sampling/Drain tube (PTFE/PP) 51328035

Teflon tube nozzle (for connection to measuring cell) 51328027

Tube nozzle special (to use with joint with thread) 51322238

Joint with thread for Sampling/Drain tube (to be used 51322239

with tube nozzle special)

Exhaust tube (1m) 51328133

Desiccator 51322012

Silica gel 1kg 105080

Protective rubber for sample inlet 51322045

METTLER TOLEDO DE40/50/51 Density Meter 85

Standard and optional equipment

18.2 Optional equipment

In use cover 51107119

Fuse T3.15A 51522100

Plastic syringe 10mL (set of 100) 71482

Certified density standards:

8 mL vials, with certificate
- Water (0.99… g/cm3 +/- 0.00001) 51338002
- Beverage sirup (1.04… g/cm3 +/- 0.00010) 51338003
- Dichlorotoluene(1.26… g/cm3 +/- 0.00002) 51338004
- Fluorocarbon (1.95… g/cm3 +/- 0.00002) 51338005

Memory Cards:
- Data, methods and sample file storage 51324004
- Alcohol 51324000
- BRIX 51324001
- API 51324002
- Coke BRIX & solids 51324009
- Viscosity correction (for DE40) 51324003

GA42 printer:
- Alphanumerical printer (serial) GA42
- Set of paper rolls (5 pcs) 00072456
- Ribbon catridge (black) 00065975
- Connection cable (not included) 51190362

PC software (for Windows 95 & 98):

- Data transfer software to EXCEL (version 7.0 or later) 51324100
- Data transfer software for DE/RE combination 51324101
- Connection cable to computer 51190362

ASU-DE sampling pump

- Sampling pump 51321500
- Waste bottle (250 mL) with lid 51322011

86 METTLER TOLEDO DE40/50/51 Density Meter

 Standard and optional equipment

AD-510 Auto Drain Unit 51321807

(requires ASU-DE)

DCU-300B Auto Rinsing Unit 51321805

(requires ASU-DE)

CHG-260B Sample Changer (60 samples) 51321208

SOP40 Sample Operation Programmer, 4 steps 51321600

SOP50 Sample Operation Programmer, 5 steps 51321601

SC24 Sample Changers

- SC24 (basic version) 51321300
- SC24H (high temperature version, electrical heating) 51321301
- SC24L (low temperature version, requires water bath) 51321302

SC24 connection sets

- Connection to DE40/50/51 51322007
- Connection to DE/RE combination 51322009

Refractometers
The following refractometers can be connected to the
DE40/50/51 density meters for simultaneous measurement
of density and refractive index)
- RE40, 4 decimal places 51320400
- RE50, 5 decimal places 51320401

Refractometer connection sets

- DE/RE connection set (basic version) 51322010
- SC24 connection set to DE/RE combination 51322009

METTLER TOLEDO DE40/50/51 Density Meter 87

MemoCard DE40/50/51 (Short Instructions)

Sample Sample No, Sample ID Function Function selection

(and number of samples if 0. Data file
Sample file ON) 1. Sample file (ON/OFF)
Viscosity correction (DE51, DE40 with 2. Method File
Memory Card Viscosity) 3. Changer
4. Factor
Report Select print format 5. Set check
off (name, value, tolerance)
short 6. Periodic (check and adjustment)
GLP (check interval)
variable (show check list)
7. Autostart
Meas.
Para Set measurement parameter 8. Card utility
Method name 9. Memory clear
Measuring temperature
Stability Setup Setup selection
Wait time 0. Interface
Sequence (ON/OFF) (Printer, RS-232)
Sampling 1. Date & Time
Drain 2. Name
Rinse-1 3. Serial/Version No.
Rinse-2 4. LCD contrast
Purge 5. International
(date format)
Calc.
Para Calculation parameter (°C or °F)
Result 6. Lock
d, d tt , d t4 7. Beep
BRIX, API, Alcohol (if selected) 8. Temperature calibration
Concentration
(enter table) Check Run instrument check
(enter coefficients) Periodic check (on check date)
Temperature compensation Daily check (all other dates)
(enter coefficients)
Decimals Calib. Perform adjustment
for d t
for d tt , d t4 (if temp.comp. select.)
BRIX, API, Alcohol (if selected) BS Backspace (erase)
for Conc. (if Conc. selected)
Rinse/
Method Method selection Purge Rinse/Purge, only for SC24 and DCU-300
Method 0 to 9 (Sequence must be ON)

Display Display selection

Density Pump Pump ON/OFF, for ASU-DE
Oscillation
BRIX, API, Alcohol (if so selected)
Conc. (if Conc. selected) Clear Clear entry (2 x to clear name)

Print Print message or parameter

Esc. Escape

Measure Start measurement

(including sample sequence Reset Return to main display
if Sequence ON)

88 METTLER TOLEDO DE40/50/51 Density Meter

 Density tables

Annex 1: Density table of dried air

The density of dried air at a specified temperature t and a atmospheric pressure of

760 mm Hg is calculated according to the following formula:

0.0012932 p (mm Hg)

d (g/cm3) = x
1 + 0.00367 x t (°C) 760

The values stored in the instrument are listed in the table below. Intermediate values are
interpolated according to the LaGrange polynomial.

Temp. Density Temp. Density Temp. Density Temp. Density

[ °C] [ g/cm3] [ °C] [ g/cm3] [ °C] [ g/cm3] [ °C] [ g/cm3]

0 0.00129 25 0.00118 50 0.00109 75 0.00101

1 0.00129 26 0.00118 51 0.00109 76 0.00101
2 0.00128 27 0.00118 52 0.00109 77 0.00101
3 0.00128 28 0.00117 53 0.00108 78 0.00101
4 0.00127 29 0.00117 54 0.00108 79 0.00100
5 0.00127 30 0.00116 55 0.00108 80 0.00100
6 0.00127 31 0.00116 56 0.00107 81 0.00100
7 0.00126 32 0.00116 57 0.00107 82 0.00099
8 0.00126 33 0.00115 58 0.00107 83 0.00099
9 0.00125 34 0.00115 59 0.00106 84 0.00099
10 0.00125 35 0.00115 60 0.00106 85 0.00099
11 0.00124 36 0.00114 61 0.00106 86 0.00098
12 0.00124 37 0.00114 62 0.00105 87 0.00098
13 0.00123 38 0.00113 63 0.00105 88 0.00098
14 0.00123 39 0.00113 64 0.00105 89 0.00097
15 0.00123 40 0.00113 65 0.00104 90 0.00097
16 0.00122 41 0.00112 66 0.00104
17 0.00122 42 0.00112 67 0.00104
18 0.00121 43 0.00112 68 0.00103
19 0.00121 44 0.00111 69 0.00103
20 0.00120 45 0.00111 70 0.00103
21 0.00120 46 0.00111 71 0.00103
22 0.00120 47 0.00110 72 0.00102
23 0.00119 48 0.00110 73 0.00102
24 0.00119 49 0.00110 74 0.00102

Chemical Handbook Fundamental Version, Rev. 3, Table 5.1 (1984).

METTLER TOLEDO DE40/50/51 Density Meter 89

Density tables

Annex 2: Density table of pure water

The instrument has stored the density of water at 760 mm Hg according to the following
table:

Temp. Density Temp. Density Temp. Density

[ °C] [ g/cm3] [ °C] [ g/cm3] [ °C] [ g/cm3]

0 0.99984 25 0.99705 50 0.98805

1 0.99990 26 0.99679 55 0.98570
2 0.99994 27 0.99652 60 0.98321
3 0.99996 28 0.99624 65 0.98057
4 0.99997 29 0.99595 70 0.97779
5 0.99996 30 0.99565 75 0.97486
6 0.99994 31 0.99534 80 0.97183
7 0.99990 32 0.99503 85 0.96862
8 0.99985 33 0.99471 90 0.96532
9 0.99978 34 0.99438
10 0.99970 35 0.99404
11 0.99961 36 0.99369
12 0.99950 37 0.99333
13 0.99938 38 0.99297
14 0.99925 39 0.99260
15 0.99910 40 0.99222
16 0.99894 41 0.99183
17 0.99878 42 0.99144
18 0.99860 43 0.99104
19 0.99841 44 0.99033
20 0.99821 45 0.99022
21 0.99799 46 0.98980
22 0.99777 47 0.98937
23 0.99754 48 0.98894
24 0.99730 49 0.98849

Chemical Handbook Fundamental Version, Rev. 3, Table 5.2 (1984).

90 METTLER TOLEDO DE40/50/51 Density Meter

 Specifications

Specifications

Type and model name Digital density meter DE40, DE50, DE51
Measuring method Oscillating U-tube
Density DE40 DE50, DE51
Measuring range d 0.0001 to 3 g/cm3 0.00001 to 3 g/cm3
Limit of error d 1 x 10-4 2 x 10-5
Repeatability d 1 x 10-4 1 x 10-5
Resolution d 1 x 10-4 1 x 10-5
Temperature
Control Peltier
Range T 4 – 90 °C 4 – 70 °C
Precision T 0.05 °C (10 to 30 °C) 0.02 °C (10 to 30°)
T 0.1 °C ( 4 to 90 °C) 0.05 (4 to 70 °C)
Viscosity compensation Memory Card (option) DE50: not possible
DE51: standard
Sample required min. 1.2 mL (manual injection with syringe)
min. 2 mL (automated operation with optional pump)
Typical meas. times 1 to 4 minutes (manual operation)
2 to 10 minutes (programmed automated operation)
Display LCD (Liquid crystal display): 7 line display, backlit
External I/O 1 RS-232C (for computer and combination)
1 RS-232C (for external printer)
1 special serial interface (for sample changer)
1 Memory Card (PCIMCIA)
Ambient condition 1) Temperature: 5 ~ 35 °C
2) Humidity: below 85 % RH (no condensation)
Power source AC 100-240 V, 50/60 Hz
Power consumption 200 W
Dimension 270 mm (W) x 400 mm (D) x 410 mm (H)
Weight 15 kg

METTLER TOLEDO DE40/50/51 Density Meter 91

FCC Rules and Radio Inteference Regulations

FCC Rules and Radio Interference Regulations

This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to both Part 15 of the FCC Rules and the radio interference regulations of
the Canadian Department of Communications. These limits are designed to provide
reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses and can radiate radio frequency
energy and, if not installed and used in accordance with the instruction manual, may cause
harmful interference to radio communications. Operation of this equipment in a residential
area is like to cause harmful interference in which case the user will be required to correct the
interference at his own expense.

92 METTLER TOLEDO DE40/50/51 Density Meter

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METTLER TOLEDO Service assures the quality, measuring accuracy and
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Printed on 100% chlorine-free paper, for the sake of our environment.

Subject to technical changes and to the availability

*P51709838* of the accessories supplied with the instruments.

© Mettler-Toledo GmbH 1999 ME-51709838 Printed in Switzerland 9906/2.12

Mettler-Toledo GmbH, Analytical, Sonnenbergstrasse 74, CH-8603 Schwerzenbach, Tel. (01) 806 77 11, Fax (01) 806 73 50,
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