R-Biopharm AG
Enzymatic testing in food analysis
A broad test portfolio for various demands
• Roche “Yellow line” as reference methods
• Enzytec™ Liquid with ready-to-use reagents
• RIDA®CUBE SCAN for single tests
• Automation with full-service package
R-Biopharm – for the safety of your analysis.
R-Biopharm AG
Introduction
Enzymatic test kits are widely used for the analysis of food products such as fruit juices, wine, beer, dairy products, egg,
meat and many others. They cover the determination of sugars (e.g. Glucose), organic acids (e.g. Malic acid), alcohols
and other food components (e.g. Sulfite). These photometric methods are based on the specificity of each enzyme for
one analyte, and the absorption of the coenzyme at 340 nm. Results are measured with a photometer, and automation
is possible.
The reference methods!
“Yellow Line” Roche Diagnostics
The “Yellow line” kits are produced by Roche Diagnostics, previously Boehringer Mannheim, with more than 40 years
experience in the production of the enzymes, which are the key element of each test. The Roche test kits have been
used and validated worldwide for several decades, with many corresponding publications. They have been selected as
reference methods by many international organizations (e.g. AOAC, IFU, IDF, ISO and OIV) and they are still the
gold standard today.
Test procedure
1. Reconstitute the lyophilized reagents with water
2. Pipette the reagent solutions and the sample into the
cuvette, then mix
3. Measure the photometric absorbance A1
4. Pipette the enzyme reagent into the cuvette, then mix
5. After 10 - 20 min incubation, read the absorbance A2
and calculate the results with the Lambert-Beer law
1 2 3 4 5
Features & benefits
• Reference quality for more than 40 years
• Approved by national and international organizations
• 31 tests for all requirements in the food industry
Stable until the last droplet!
Enzytec™ Liquid and Color
These reagents are all liquid and ready-to-use, which brings a huge improvement in the lab organization:
• In small laboratories, it is possible to test a few samples and store the reagents for further usage, since the original
shelf-life remains valid.
• In large laboratories with automation, the reagents can be placed directly on any biochemistry analyzer, and stay
refrigerated on board for true random-access.
Features & benefits
• Reagents are liquid and ready-to-use (no reconstitution)
• Easy handling with same pipeting scheme for all tests
• Reagents are stable up to the expiry date,
even after opening
• Easy and safe use on biochemistry analyzers
Cost reduction!
Automation
Automation is now largely used in enzymatic testing, thanks to numerous biochemistry analyzers coming from the
clinical diagnostics. Automation allows reducing handling time and handling errors. But for enzymatic testing it also
reduces reagent costs, because the test volume is reduced to 300 µl instead of 3 ml for the manual procedure.
All enzymatic kits, including the Roche line, can be adapted to any instrument with applications available on request.
R‑Biopharm promotes two liquid-handling robots, BOLT™ and ThunderBolt®, with full-service package including
instrument, maintenance, reagents and applications.
Features & benefits
• Walk-away and multi-batch testing for large sample series
• Reagents always on-board for random access
• Automatic sample pre-dilution
• No handling errors and better reproducibility
• Cost reduction (handling time and reagent consumption)
Single-testing anytime, everywhere!
RIDA®CUBE SCAN
The RIDA®CUBE SCAN is a small walk-away instrument that allows single-testing in production facilities or in small
laboratories. The instrument is controlled from a tablet with a simple app. Each test-kit contains 32 single-test
cartridges and one RFID card with all test specific data, so there is no need to enter any settings. Pipette the sample
into the tube and place it into the instrument, the entire test will be performed automatically. Results are displayed on
the tablet, they can also be exported to a computer.
Procedure
1. Place the RFID card on the instrument.
2. Enter sample identification into the tablet app
3. Pipette the sample into the tube
4. Close the tube with the cap and insert it into
the instrument
1 2 3 4
Features & benefits
• Small and portable device (16 x 13 x 14.5 cm)
• Ready-to-use reagent cartridges for single-testing
• RFID card including all test settings
• Only one pipeting step, result after a few minutes
• Maintenance free (no pipeting device inside the instrument)
R-Biopharm AG
Enzymatic analysis – R-Biopharm test kit portfolio
“Yellow Line” Enzytec™ RIDA®CUBE SCAN
Roche Diagnostics Liquid, ready to use and Single-test cartridges
The reference method stable reagents
Acids
Acetic acid (340 nm) 10148261035 E5226 RCS4226
L-Ascorbic acid (578 nm) 10409677035
Citric acid (340 nm) 10139076035
Formic acid (340 nm) 10979732035
Gluconic acid (340 nm) 10428191035
Glutamic acid (492 nm) 10139092035
D-3-Hydroxybutyric acid (492 nm) 10907979035
D-Isocitric acid (340 nm) 10414433035
D/L-Lactic acid (340 nm) 11112821035 E8240 RCS4240
L-Lactic acid (340 nm) 10139084035 E8260 RCS4260
D-Malic acid (340 nm) 11215558035
L-Malic acid (340 nm) 10139068035 E8280 RCS4280
Oxalic acid (580 nm) E2100
Succinic acid (340 nm) 10176281035
Tartaric acid (520/546 nm) E3100
Sugars
ß-Glucan (546 nm) E3500/E3550
D-Glucose (340 nm) 10716251035 E8140 RCS4140
D-Glucose/D-Fructose (340 nm) 10139106035 E8160 RCS4160
Lactose/D-Galactose (340 nm) 10176303035 E8110/E8120
Lactose/D-Glucose (340 nm) 10986119035 E8130
Maltose/Sucrose/D-Glucose (340 nm) 1113950035
Raffinose (340 nm) 10428167035
Starch (340 nm) 10207748035
Sucrose/D-Glucose (340 nm) 10139041035 E8180 RCS4180
Sucrose/D-Glucose/D-Fructose (340 nm) 10716260035 E8190 RCS4190
Others
Acetaldehyde (340 nm) 10668613035
Ammonia (340 nm) 1112732035 E5390
Urea/Ammonia (340 nm) 10542946035
Cholesterol (405 nm) 10139050035
Copper (580 nm) E2400
Ethanol (340 nm) 10176290035 E8340 RCS4340
Glycerol (340 nm) 10148270035 E5360
Iron (580 nm) E2300
Nitrate (340 nm) 10905658035
D-Sorbitol/Xylitol (492 nm) 10670057035
Free Sulfite (340 nm) E8610 RCS4610
Total Sulfite (340 nm) 10725854035 E8600 RCS4600
Standards
Alcohol standard E5420
Multi-acid standards (low and high) E1240/E1241
Multi-sugar standards (low and high) E5440/E5450
05/2017
R-Biopharm AG • An der neuen Bergstraße 17, 64297 Darmstadt, Germany • E-mail: [email protected] • www.r-biopharm.com
