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BIOCHEMICAL AND PATHOLOGICAL EFFECTS OF PALM, MUSTARD AND

SOYBEAN OILS IN RATS

Article  in  Bangladesh Journal of Veterinary Medicine · July 2018

DOI: 10.3329/bjvm.v16i1.37385

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Bangl. J. Vet. Med. (2018). 16(1): 107–114 ISSN: 1729-7893 (Print), 2308-0922 (Online)

BIOCHEMICAL AND PATHOLOGICAL EFFECTS OF PALM, MUSTARD

AND SOYBEAN OILS IN RATS
M. S. Hoque1, M. E. Kabir1, M. M. Hasan2, M. T. Rahman3, M. Rashid1, T. Ruba1,
M. H. Rahman1, A. B. M. J. Uddin1 and M. M. Hossain1*
1
Department of Pathology,2Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural
University, Mymensingh-2202, Bangladesh; 3Department of Para-clinical courses, Faculty of Veterinary &
Animal Sciences, Gono Bishwabidyalay (Gono University), Dhaka, Bangladesh.
ABSTRACT
The investigation was conducted to determine the effects of different edible oils in experimental animals. A total of 36 male
Long Evans rats of one month age were randomly divided into four equal groups. Rats of Group A were kept as control by
feeding rat pellet. Rats of Group B, C and D were fed rat pellet by mixing of palm, mustard and soybean oils respectively, at
the dose of 15 % in feed for 6 months. The methods included determination of weight gains, lipid profiles and
histopathological lesions in different organs: aorta, liver, heart and kidneys at 0 day, 3 months and 6 months interval. All
experimental rats exhibited progressive weight gain during the research period and soybean oil treated group showed the
highest significant (P<0.01) body weight gain (153 %) but mustard oil resulted significant (P<0.05) increased liver weight
(4.557g) after 6 months. Soybean oil showed significant (P<0.01) increased total cholesterol (204.25 mg/dl), HDL (53.15
mg/dl) and LDL (113.06 mg/dl) than other groups. Triglyceride levels of all oil treated groups were significantly (P<0.05)
lower than control group. Histopathology revealed that palm oil fed group had fatty liver, narrowed blood vessel and thickened
aorta of heart. Soybean oil fed group also showed narrowed blood vessels but mustard oil fed group showed no noticeable
change in the mentioned vital organs. After the investigation, mustard oil proved comparatively better than palm and soybean
oil.
Keywords: Edible oil, body weight, lipid profile, histopathology, rats
INTRODUCTION
At present, cardiovascular diseases are of great concern for human being and companion animals due to
changing of socioeconomic condition as well as eating and drinking behavior. Among the risk factors (excess
fat/lipid intake, age, lack of exercise, smoking, drinking alcohol, diabetes, etc.), intake of high lipid/fat
containing food is of prime importance for cardiovascular diseases. Lipids play an important role to supply
energy for meeting the calorie requirements and act as storage and transport forms of metabolic fuel. Each gram
of fat or oil supplies 9 kcal energy which is about double than those of energy provided by proteins or
carbohydrates. Current recommendations are to limit dietary fat and cholesterol consumption to 30 % or less of
total calories or 300 mg or less per day (Baron and Browner, 1998) and saturated fat should not be made more
than 15 % of the total fat intake. High fats and oils intake with diet cause diseases like atherosclerosis, ischemic
heart disease, chronic nephritis, obesity, hypertension, diabetes etc. which are reported in both human and
animals (Kagawa et al., 1998; Austin et al., 2000; Jakobsen et al., 2009; Soler and Ruiz, 2010; Xenoulis and
Steiner, 2010). The presence of cholesterol, saturated fatty acids (Wood et al., 1966) and trans fatty acids in fats
and oils increase the risk of coronary heart diseases by increasing the blood cholesterol (Lichtenstein, 1988).
In Bangladesh, epidemiological study on heart diseases in man is related with dyslipemia or obesity were
reported, (Zaman et al., 2007; Sayeed et al., 2010; Islam et al., 2002; Islam and Majumder, 2012) but
information on heart diseases of companion animals (i.e., dog, cat, etc.) is not yet to be found. The data of high
quantity and prolong use of palm (saturated fatty acids), mustard oil (monounsaturated fatty acids) and soybean
oil (polyunsaturated fatty acids) in humans and laboratory animals are very scanty in Bangladesh (Rahman,
2009). As it is difficult to carry out research on human and companion animals (dogs and cats), the laboratory
animals (rats) were chosen as the experimental model for this study. Therefore, the present experiment was
designed to study the effect of selected edible oils (palm, mustard and soybean oils) in body and organs weight
gain, changing on lipid profiles and examine the pathological changes of vital organs in rat at different time
intervals.

*Corresponding e-mail address: [email protected]

Copyright  2018 Bangladesh Society for Veterinary Medicine All rights reserved 0418/2018
Hoque and others

MATERIALS AND METHODS

The experiment was conducted in the Department of Pathology, Bangladesh Agricultural University (BAU),
Mymensingh during the time of February to August 2016. The following procedures were adopted for
conducting the experiment.
Statement of Animal Ethics
In this study no animals were suffered from hazards for the experiment and had followed ethical guide for
care and use of laboratory animals. The procedure of the experiment was approved by the Animal Welfare and
Ethical Committee on Laboratory Animal Use of the Faculty of Veterinary Science, Bangladesh Agricultural
University.
Experimental rats and diets
A total number of 36 one month aged Long Evans rats were purchased from International Center for
Diarrhoeal Disease Research, Bangladesh (ICDDR’B). The rats were randomly divided into four equal groups
(A, B, C & D. 9 rats in each group). All groups of rats were housed in compartmented metallic cages. The rat
cages were kept in well ventilated room with natural day and light. Temperature (28±2°C) and humidity (70-80
%) of laboratory were always kept under care. Initially, rats were fed with ordinary rat pellet for 7 days to adjust
into new environment. The feeds were purchased from ICDDR’B, Dhaka and the test oils (soybean, palm, and
mustard oil) were purchased from local market of Mymensingh, Bangladesh. Among four groups, A group was
kept as control by feeding normal rat pellet and other three groups (B, C & D) were fed rat pellet by mixing
palm, mustard and soybean oils respectively, at the dose of 15 % (Gaiva et al., 2003). The experiment was
continued for 6 months. Body weight, organ weight and histopathology samples (heart, liver, kidney and aorta)
of each rat from all groups were taken at 0 day of experiment, 3 months and 6 months. Blood was collected for
lipid profile analysis at 0 day of experiment, 3 months and 6 months. Proper hygienic and sanitary measures were
also adopted during the experimental period and had followed ethical guide for care and use of laboratory
animals.
Weight gain
Initial body and organ weight of each rat was measured with the help of an electric balance. Body and organ
weight was taken at 0 day, 3 months and 6 months age.
Weight gains (g) = Mean final weight (g) − Mean initial weight (g)
Percent weight gain (g) = [Mean final weight (g)-Mean initial weight (g)] / (Mean initial weight) ×100
Biochemical studies
Collection of blood and serum preparation
For biochemical study, the experimental rats were starved overnight at 0 day, 3 months and 6 months of age.
Anesthesia was done with chloroform using self-made gas chamber and 2-3 ml of blood was collected in the
sterile glass test tubes directly from heart after opening. The blood containing tubes were placed in slanting
position at room temperature for 6 hours. The tubes were then incubated overnight in the refrigerator (4° C). The
serum samples were separated and centrifuged to get rid of unwanted blood cells where necessary. Serum
samples were stored for biochemical analysis of lipid profiles.
Analysis of Lipid Profiles
The biochemical parameters of lipid profile like total cholesterol (TC), triglycerides (TG), serum cholesterol
HDL (high density lipoprotein) and serum cholesterol LDL (low density lipoprotein) were determined in the
central lab of BAU using different kits (Crescent diagnostics, cat no. Cs. 603 and Atlas medical) and where LDL
level was measured by the Friedwald equation (Friedwald et al., 1972) as follow:
LDL cholesterol (mg/dl) = Total cholesterol - HDL cholesterol - (Triglyceride/5)
Histopathology
The liver, kidney, heart and aorta from each group of rats were collected in 10 % neutral buffered formalin
for 15 days. The well fixed tissues were processed, sectioned and stained as per standard procedure (Luna, 1968)
in Department of Pathology, Bangladesh Agricultural University, Mymensingh.
Statistical analysis
The body weight gains of the experimental rats of different groups were computed by performing analysis of
covariance in randomized complete block design (Gomez and Gomez, 1984). The mean organ weights of the
different groups were compared with the control by Dunnett’s test procedure (Steel and Torrie, 1980).

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 Biochemical and pathological effects in rats

RESULTS AND DISCUSSION

This study was conducted on Long Evans rats to observe the effects of supplementing diets with marked edible
oils soybean, palm and mustard oil on growth, lipid profile and histopathological changes in some selected
organs for a period of 6 months. Body and organ weights were recorded at 0 day, 3 months and 6 months of age.
Lipid profile and histopathological investigation were carried out at same age respectively after sacrificing the
rats.

Growth performance

Body weight: The body weight gain percentages in different groups are shown in Table 1. All the edible oil
treated rat groups had significantly (P<0.01) increased body weight gain than control group. After 3 months,
control group weight gain percentage was 51, whereas in palm, mustard and soybean weight gain percentages
were 102, 104.44 and 103.78 respectively. The highest weight gain occurred in soybean oil fed treated rat group
(153 %) after 6 months of age, less than in palm (131.63 %), mustard (109 %) and control group (51 %). Besides,
as shown in Table 2, there was significant (P<0.05) difference in body weight gain among the edible oil
treatment groups. The observed findings supported by the experimental studies where edible oil especially
soybean was effective in increasing body weight gain (Rashid et al., 1998; Barek, 2002; Gaiva et al., 2003;
Rahman et al., 2012) in different animals.

Table 1. Comparison of body weight gain percentage at 3 months and 6 months of age in different treatment
groups by Dunnett’s test procedure
Treatment % body weight % body weight
Serial
Group gain at 3 months gain at 6 months
1 Control 51 51
2 Palm 102** 131.63**
3 Mustard 104.44** 109**
4 Soybean 103.78** 153**
**
indicates that values having double asterisk within the same column differ significantly (P<0.01).

Table 2. Analysis of variance (ANOVA) for body weight gains of the experimental rats
Source of variance Sum of Squares Degree of freedom Mean Square Sig.
Between Groups at 3 month 1068.917 3 356.306 .012
Between Groups at 6 month 9843.667 3 3281.222 .001
From this table, it could be interpreted that there is existence of significant (P<0.01) difference among the
treatment groups.

Organ weight: The proportional organ weight percentage is shown in relation with the body weight of the rats of
different treatment groups at day 0, 3 months and 6 months in Table 3. The study findings revealed that edible oil
treatment had impact on organ weight gain in rats. From Dunnett’s test, heart weight showed no statistically
significant difference in edible oil treated groups compared to control group in both at 3 months and 6 months of
age. Mustard oil had significant (P<0.05) impact on increasing liver (4.557g) weight where palm oil significantly
(P<0.05) reduced the liver weight (2.846g), than control group (3.497g) after at the age of 6 months. In kidney,
palm and mustard oil significantly (P<0.05) reduced the kidney weight from their initial value, but soybean oil
treated group significantly (P<0.05) increased the weight at 3 months of age and found significant (P<0.05)
reduced weight at 6 months of age. Saha et al. (2005) reported that higher liver weight gain in mustard oil fed
group than palm and soybean, where this study had similar findings compared with control and other groups.
Palm oil caused reduction of kidney weight in rat, this result also corroborated by Jaarin et al. (2015).

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Hoque and others

Table 3. Comparison of the organ weight proportional percentage in relation to body weight in 0 day, 3 months
and 6 months of age of different treatment group by Dunnett’s test procedure
Experimental Group
Organ weight gain Time
Control Palm Mustard Soybean
0 day 0.380 0.379 0.377 0.373
Heart (mg) 3 months 0.330 0.359 0.338 0.340
6 months 0.311 0.325 0.331 0.312
0 day 4.080 4.110 4.090 4.130
Liver (g) 3 months 3.711 3.310 4.660* 3.730
6 months 3.497 2.846* 4.557* 3.424
0 day 0.458 0.445 0.450 0.461
Kidney- L (mg) 3 months 0.363 0.313* 0.408 0.465*
6 months 0.342 0.332 0.398* 0.427*
0 day 0.472 0.462 0.471 0.467
Kidney- R (mg) 3 months 0.355 0.331* 0.331* 0.471*
6 months 0.334 0.341 0.416* 0.433*
*
indicates that values having asterisk within the same column differ significantly (P<0.05).

Lipid Profile Analysis

Table 4 reveals the comparative analysis of lipid constitutes of experimental edible oil treated rat groups and
control group in different time periods. The changes of lipid profiles value were significant in edible oil treated
rat group than control group. At 0 day, the findings were similar in all experimental groups. The serum
cholesterol level of three treatments were significantly (P<0.05) lower than the control group (189.1±4.19965)
after 3 months of age, where soybean oil treated group showed the lowest value (165.5733±5.89714) and this
findings agree with the results of other experimental studies (Islam et al. 2009, Erukainure et al. 2016). In
contrast, palm and soybean oil caused significant (P<0.05) increase of cholesterol level and mustard oil caused
significant (P<0.01) decrease of cholesterol level than control group after 6 months of age (Table-04). In
comparing with the 3 months and 6 months cholesterol level values of edible oil treatment group and control
group, the study revealed that a sharp contrast in between the results of both treatment groups and control group
but it agrees with the findings of Fernandez et al. (1996) and Choi et al. (1993). At 3 months, soybean oil caused
significant (P<0.05) decrease of TG (150.96±3.66) than control group (179.75±3.92) whereas palm and mustard
oil caused significant (P<0.01) increase of TG (185.31±4.92 & 193.23±2.97 respectively). But at 6 months, all
the oil groups caused significant (P<0.01) decrease of TG. The study results of Islam et al. (2009) and
Erukainure et al. (2016) having similar results of this experiment after 6 months.
The result of the Dunnett’s test indicates that palm and soybean oil caused significant (P<0.05) increase HDL
level at 3 and 6 months but mustard oil showed no significant changes than control group (Table-04). Islam
(2002) also reported that the value of HDL increased in case of giving 2 % soybean supplement with feed in
broiler. In this study, LDL level decreased significantly (P<0.05) in all edible oil treated groups after 3 months
whereas soybean oil (113.06±9.32) and palm oil (99.04±9.09) caused significant (P<0.05) increased level of
LDL after at 6 months (Table 4). LDL value of different treatment rat groups agreed with the result of Islam et
al. (2002) after 3 months but it was different at 6 months of age.

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 Biochemical and pathological effects in rats

Table 4. Comparisons of lipid profile (TC, TG, HDL and LDL) levels at 0 day, 3 months and 6 months of age in
different treatment groups by Dunnett’s test procedure

Experimental Group
Lipid Profile Time
Control Palm Mustard Soybean
0 day 113.99 ± 2.18 114.2±2.11 112.12±1.89 114 ±2.02
TC mg/dl 3 months 189.1±4.19 187.15±1.86* 171.67±1.99** 165.57±5.89**
6 months 182.23 ±1.06 186.00 ±4.71* 175.18±4.67** 204.25±3.43*
0 day 76.13 ± 2.38 75.15 ±1.85  74.17±2.12 78.24 ±2.11
TG mg/dl 3 months 179.75±3.92 185.31±4.92* 193.23±2.97* 150.96±3.66**
6 months 196.17±7.49 186.36±5.74* 182.76±8.04* 176.64±7.68*
0 day 42.14 ± 1.05 41.13 ± 1.25 40.12 ± 2.10 41.23± 2.12
HDL mg/dl 3 months 40.01±5.58 47.12±6.87* 38.87±.71 46.85±4.84*
6 months 41.85±2.78 46.68±4.52* 39.54±2.32 53.15 ± 27**
0 day 99.64 ± 1.82 98.50 ±1.45 99.50±1.25 98.32±1.02
LDL mg/dl 3 months 105.36±3.94 96.20±6.40* 97.47±5.54* 86.53±7.53**
6 months 94.36±12.95 99.04±9.09* 93.07±9.62 113.06±9.32**

Values having asterisks within the same column differ significantly (** indicates P<0.01, * indicates P<0.05).

Histopathological Examination
The histopathological studies of heart, liver, aorta and kidney tissues were performed to investigate the effects
of different oil-supplementation in rats of 3 and 6 months of age. Among all the edible oils, the palm oil treated
group showed only significant lesions on heart blood vessels, liver and aorta. In heart, the blood vessels wall had
become thickened in palm and soybean oil treated rat groups (Figures 1 & 2). The aorta wall had become
thickened in the palm oil treated rat group which indicates the arterioscelorisis (Figure 3). Further, section of
liver of control group, soybean oil and mustard oil treated rat groups showed normal histology without showing
any detectable lesions but severe fatty changes in liver were found in palm oil group (Figure 4). Few studies
reported that palm oil was responsible for degenerative changes in hepatocytes, congestion of central vein and
fatty changes in liver (Jaarin et al., 2015; Saha et al., 2005), and these reports upheld the result of this
experiment. In addition, the present experiment showed only mild inflammation in kidney of mustard oil fed rat
group where other oil fed groups showed normal histoarchitecture. So histopathologically palm oil proved as the
worst and mustard oil proved as the best one among these three groups.
From the present experiment it could be concluded that edible oils have effect on growth performance and
lipid profiles in rat. After the investigation, on the basis of TC, HDL, LDL level and histopathology, it is clear
that mustard oil had less harmful effect on body than the other oils although it was observed that the results of 3
months age were quite different than the result of 6 months of age. There may have age relation or something
else that caused this variation. However, it is a preliminary work and in future this would require further study
for better explanation in this regard.

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Hoque and others

Figure 1. Section of heart: Palm oil treated rats group. Figure 2. Section of heart: Soybean oil treated rats
Arrow mark Shows narrowing of blood vessel. (H & E group. Arrow mark shows narrowing of blood vessel.
staining, 10X) (H & E staining, 10X)

Figure 3. Section of aorta: Palm oil treated rats Figure 4. Section of liver: Palm oil treated rats group.
group. Arrow shows thickening of aorta. (H & E Arrow shows severe fatty changes. (H & E staining,
staining, 10X) 10X).

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 Biochemical and pathological effects in rats

ACKNOWLEDGEMENTS
The authors highly acknowledged the Bangladesh Agricultural University Research System (BAURES),
Mymensingh, Bangladesh for providing the fund to carry out the research.

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