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931.01 Fosforo en Plantas

This document provides a method for determining the phosphorus content in plant materials using a micro method. The key steps are: 1) Ashing the plant material at 500°C to obtain a gray ash. 2) Dissolving the ash in acid and evaporating to dryness to prepare the test solution. 3) Adding reagents including ammonium molybdate, hydroquinone, and sodium sulfite to the test solution to produce a colored complex, with the color intensity being proportional to the phosphorus content. 4) Measuring the absorbance of the solution at 650nm using a spectrophotometer to determine the phosphorus percentage.

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lizeth rico quintero
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144 views1 page

931.01 Fosforo en Plantas

This document provides a method for determining the phosphorus content in plant materials using a micro method. The key steps are: 1) Ashing the plant material at 500°C to obtain a gray ash. 2) Dissolving the ash in acid and evaporating to dryness to prepare the test solution. 3) Adding reagents including ammonium molybdate, hydroquinone, and sodium sulfite to the test solution to produce a colored complex, with the color intensity being proportional to the phosphorus content. 4) Measuring the absorbance of the solution at 650nm using a spectrophotometer to determine the phosphorus percentage.

Uploaded by

lizeth rico quintero
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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3.4.11 that as material approaches dryness it tends to char.

If contents
AOAC Official Method 931.01 become too viscous for further drying on bath, complete drying on
Phosphorus in Plants hot plate.
Micro Method Cover crucible, transfer to cold furnace, and ignite 6 h at 500°C, or
First Action 1931 until even gray ash is obtained. (If necessary, cool crucible, dissolve
Final Action
ash in a little H2O or alcohol–glycerol, evaporate to dryness, and
A. Reagents return uncovered to furnace 4–5 h longer.) Cool, take up with HCl (1
(a) Phosphorus standard solution.—0.025 mg P/mL. Dissolve + 4), and transfer to 100 mL beaker. Add 5 mL HCl and evaporate to
0.4394 g pure dry KH2PO4 in H2O and dilute to 1 L. Dilute 50 mL of dryness on steam bath to dehydrate SiO2. Moisten residue with 2 mL
this solution to 200 mL. HCl, add ca 50 mL H2O, and heat few minutes on bath. Transfer to
(b) Ammonium molybdate solution.—Dissolve 25 g ammonium 100 mL volumetric flask, cool immediately, dilute to volume, mix,
molybdate in 300 mL H2O. Dilute 75 mL H2SO4 to 200 mL and add and filter, discarding first portion of filtrate.
to ammonium molybdate solution.
C. Determination
(c) Hydroquinone solution.—Dissolve 0.5 g hydroquinone in
100 mL H2O, and add one drop H2SO4 to retard oxidation. To 5 mL aliquot filtrate in 10 mL volumetric flask, add 1 mL
(d) Sodium sulfite solution.—Dissolve 200 g Na2SO3 in H2O, ammonium molybdate solution, rotate flask to mix, and let stand few
dilute to 1 L, and filter. Either keep this solution well stoppered or seconds. Add 1 mL hydroquinone solution, again rotate flask, and add
prepare fresh each time. 1 mL Na2SO3 solution. (Last 3 additions may be made with Mohr
B. Preparation of Test Solution
pipet.) Dilute to volume with H2O, stopper flask with thumb or
forefinger, and shake to mix thoroughly. Let stand 30 min, and
To 1 or 2 g test portion in small porcelain crucible, add 1 mL
measure A with spectrophotometer set at 650 nm. Report as percent P.
Mg(NO3)2 solution [dissolve 950 g P-free Mg(NO3)2⋅6H2O in H2O
and dilute to 1 L], and place on steam bath. After few minutes, References: JAOAC 14, 216(1931).
cautiously add few drops HCl, taking care that gas evolution does J. Biol. Chem. 59, 255(1924).
not push solids or test solution over edge of crucible. Make 2 or 3
further additions of few drops HCl while test solution is on bath so CAS-7723-14-0 (phosphorus)

 2005 AOAC INTERNATIONAL

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