Scribd
Upload
696 views2 pages

SGPT ASAT Kit Mod IFCC Method PDF

Uploaded by

Dharmesh Patel
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF or read online on Scribd
696 views2 pages

SGPT ASAT Kit Mod IFCC Method PDF

Uploaded by

Dharmesh Patel
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF or read online on Scribd
You are on page 1/ 2
SGPT (ALAT) KIT (Reiman &Frankts method) Fert trot f OPT AAT yen, ores Ue On sonay Sorte burdna wit tte bt sma aed Siete frase tet abe ee eat ap cee Peas ose san hc Princpl SGPT coms L-Arine and Ketogarate to Pyrat ‘and Gltarate. The Pyruvate formed reacts wih LaDintophony hydazne to produce a hydezone ‘eretv. whch nen alkane mesum prduces a brown oloued complex whose vlenly Is massed The reason doe rot obey Bao’ ou and hace a carson cues plated using a Pyruvate standard. The actly of SGPT ALATisreadofinscalbratoncane. Llane S6PT Parte aXetogutarate pT Lowanate Pywate Aang 2 Dion eee yarazone 240WH Meds (Browneseueccemplx) Normareference values Senn = §-35Unisn Ts recommended that each boar esablah fs own serralranga epresening spate ppuaten. Contents snassays BDASSAyS Li:SubsvaleRoagort 25 ml SO LZ: DNPH Reager mom LE:NGOHReagen(iN) 25m SD S :Pyvate Stntaré(2mM) Sm Sm Storage stability Contents ar sable at 28°C te expiry mentoned on ‘helabos. Sodium Hydraxde canbe sted ali he ‘xy ertond. Reagent Preparation ‘reagents are ready to use except NAOH Reagent) whichis tobected | 10vihdsldietrised te, Working NaOH reagent: Diu th Sodium Hydox to 250 lot for eer Om of NaOH Reagent 4) G80 ofwat. The Worn Sedum Hysroiareagortislaiaat RT.thtaeoxprymentoned, aplastic tle Sample material Soran Fre romtomniyss. SGPT LAT sroportedtobe stabainsaruntarsdaysat 23°, Procedure Wavelength fier Teperatire Ughtpath 501 546m) Green SPCERT, ten Piotngofthe Calibration cuve Pipette nofv dean dy test tubes labeled a 1,234 a5 aaitonsenee [12348 Enoki ind [tatoo o_o Dani 0 010 010 910 410 apereanmei | 00 950 050-050 950 Tires dow TART Wey NaOH Reape] 60060 8 GOSS ox welled alow oslard aR fr 10min. Measure the absorancasolthalubes2-Sagsst Lbs 1 (Blan) Plt ‘8 gaph ofthe absorbances of bes 2-§ on he'Y axs ‘praise comospenang Ete eciviyon oan, Assay: Pips in clean dry tst ubes abled as Blank (3) & Test 1 ‘tion Ca Sequence oe oc Teale 57 fo aoe Sane Lan ix eis SFE er PH Reap LZ) ts) | 05 Twa a lw Sane wT. ring NeoH Reape) sco | sca ox wall and allow sland a RT or 10min. Measure the absoranoes of ine Tet (7) against Bank (8) andreas the ‘chiyftetastromecaltratoncuve plated eri Note (One sal blanks suficarifoceachasay series. I Eneye acy exceeds 150 Uimidisthe sale wih

You might also like