Chhattisgarh Swami Vivekanand Technical University, Bhilai (C.G.

)
Scheme of Teaching & Examination

BE IV Semester Biotechnology

Period per Scheme of Exam

S.N Subject Total Credit
Subject week L+(T+P)/2
o Board of Study Code Theory/Practical Marks
L T P ESE CT TA
1 Biotechnology 318411(18) Cellular And Molecular Biology 4 0 - 80 20 20 120 4
2 Biotechnology 318412(18) Recombinant DNA Technology 4 0 - 80 20 20 120 4
3 Biotechnology 318413(18) Food Biotechnology 3 1 - 80 20 20 120 4
4 Chem. Engg 318414(19) Stoichiometric Calculations 3 1 - 80 20 20 120 4
5 Chem. Engg 318415(19) Transport Processes 4 1 - 80 20 20 120 5
6 Chem. Engg 318416(19) Thermodynamics and 4 0 - 80 20 20 120 4
Biochemical Reaction
Engineering
7 Biotechnology 318421(18) Cellular And Molecular Biology - - 3 40 - 20 60 2
Lab
8 Biotechnology 318422(18) Recombinant DNA Technology - - 3 40 - 20 60 2
Lab
9 Chem. Engg 318423(19) Transport Processes Lab - - 3 40 - 20 60 2
10 Chem. Engg 318424(19) Thermodynamics and - - 3 40 - 20 60 2
Biochemical Reaction
Engineering Lab
11 Humanities etc. 300425(46) Health, Hygiene and Yoga - - 2 - - 40 40 1
12 Library - - 1
Total 22 3 15 640 120 240 1000 34

L: Lecture, T: Tutorial, P: Practical, ESE: End Semester Exam, CT: Class Test, TA: Teachers Assesment

Note (1) : Duration of all theory papers will be of Three Hours.

Note (2) : Industrial Training of six weeks is mandatory for B.E. student. It is to be completed in two parts. The first part will
be in summer after IV sem. after which students have to submit a training report which will be evaluated by the
college teachers during B.E. V sem.
 Chhattisgarh Swami Vivekanand Technical University, Bhilai (C.G.)

Semester : IV Semester B.E Branch: Biotechnology

Subject: Cellular and Molecular Biology Code: 318411(18)
Total Theory Periods: 40 Total Tut Periods: Nil
Total Marks in End Semester Exam: 80
Minimum number of class test to be conducted: 2

Unit 1:- Cell structure, function of organelles, principle of membrane organization, membrane
protein. Cell division- mitosis and meiosis. Cell cycle and its regulation. Cytoskeleton–
various types and their functions.
[ No. of Periods – 8 ]
Unit 2:- Transport across cell membranes– passive and active transport, permease, sodium-
potassium pump. ATP dependent proton pump, ion channel and electrical properties of
membranes.
[ No. of Periods – 8 ]
Unit 3:- Signal Transduction– signal amplification, cyclic AMP, roll of inositol phosphate as
messenger, roll of G-protein in signal transduction, role of Ca2+ ion in cell signaling.
Enzyme linked cell surface receptors.
[ No. of Periods – 8 ]
Unit 4:- Gene Organization– split gene, overlapping gene, pseudogenes, transposons and
retrotransposons, mechanism of RNA splicing, retrovirus, DNA methylation.
[ No. of Periods – 8 ]
Unit 5:- Genetic Transfer- conjugation, transduction, transformation. Multiple alleles and types of
blood groups, antigens.
[ No. of Periods – 8 ]
Name of Text Books:
1. Freifelder D. [ “Molecular Biology” ] Jones Barat lelt Publishers Icn. 1987.
2. Gene VIII Benjamin Lewin .
3. Genome by T. A. Brown, John Wiley & Son’s, RD PTE Limitel .
Name of Reference Books:
1. Cell & Molecular Biology “concepts & Experiments” Geratd Karp, John Wiley &
Son’s.
2. The Cell- A molecular approach, Gn Cooper Asm Prers.
3. Cell and Molecular Biology by S. C. Rastogi
4. Cell Biology, Genetics, Molecular Biology, Evolution and Ecology by P. S. Verma
and V. K. Agrawal
5. Concepts in Molecular Biology by Rastogi et al.
 Chhattisgarh Swami Vivekanand Technical University, Bhilai (C.G.)

Semester : IV Semester B.E Branch: Biotechnology

Subject: Recombinant DNA Technology Code: 318412(18)
Total Theory Periods: 40 Total Tut Periods: Nil
Total Marks in End Semester Exam: 80
Minimum number of class test to be conducted: 2

Unit 1:- Basic concepts of Genetic Engineering– isolation, identification and characterization of
DNA, plasmids and its uses. Tools of genetic engineering– cloning vectors, restriction
enzymes, modifying enzymes- DNA ligase, polymerases, etc.
[ No. of Periods – 8 ]

Unit 2:- Vectors- phasmids, phage vector- M13, λ, phasmids, cosmids, phagmid, Artificial
chromosomes- BAC, YAC, MAC, Shuttle vector.
[ No. of Periods – 8 ]

Unit 3:- Gene Cloning – isolation of desired gene, preparation of rDNA and its integration into
host cell, selection and screening of transformants.
[ No. of Periods – 8 ]

Unit 4:- Gene Library- cDNA preparation, cDNA library, genomic DNA library. Amplification of
gene library, difference between cDNA Library and genomic library.
[ No. of Periods – 8 ]

Unit 5:- Preparation and application of molecular probes, DNA probes, RNA probes, radioactive
and non-radioactive labelling of DNA. Methods of gene transfer- natural and artificial.
[ No. of Periods – 8 ]
Name of Text Books:
1. Principals of gene Cloning By Old & Primrose
2. Molecular Cloning By Sambrook at el
Name of Reference Books:
1. Gene Cloning & DNA Analysis “An Introduction” By T.A. Brown.
2. From Gene to genomes “Concepts & Application of DNA Technology” By J.W. Dale
& M.V. Schartz.
3. Biotechnology by B. D. Singh, Kalyani Publishers
 Chhattisgarh Swami Vivekanand Technical University, Bhilai (C.G.)

Semester : IV Semester B.E Branch: Biotechnology

Subject: Food Biotechnology Code: 318413(18)
Total Theory Periods: 30 Total Tut Periods: 10
Total Marks in End Semester Exam: 80
Minimum number of class test to be conducted: 2

Unit 1:- Role and significance of micro-organism in food. Intrinsic and extrinsic parameters of
food that affect microbial growth. Micro–organisms in fresh meat and poultry, processed
meat, sea food. Food spoilage.
[ No. of Periods – 6+2]
Unit 2:- Brewing, malting, massing, use of hops in beer industries, primary and secondary
fermentation. Beer, wine and distillation spirit. Cheese production , various dairy
products. Vinegar and Soya sauce production.
[ No. of Periods – 6+2]
Unit 3:- Food Preservation– using irradiation, characteristics radiations of interest in food
preservation. Principles underlying the destruction of microorganisms by irradiation.
Food preservation using various temperature means, chemical preservatives used in food
preservation.
[ No. of Periods – 6+2]
Unit 4:- Use of enzyme in food industry, use of lactase in dairy industry, enzymes in fruit juice
and brewing industries. Microbial Biomass (Mushroom – Agaricus bisporus, pleurotus
species, Volvarrilla spp.).
[ No. of Periods – 6+2]
Unit 5:- Single cell protein– microorganisms, substrates used, production of SCP, biomass
recovery. Nutritional and safety evaluation. Advantages of SCP.
[ No. of Periods – 6+2]

Name of Text Books:

1. Biotechnology by B.D. Singh, Kalyani Publishers.
2. Food Microbiology – fundamentals & frontiers by M.P. Doyle, L.R. Beuchal &
Thorna J. Montvillle(2001) A.S.M. Press.
Name of Reference Books:
1. Modern food microbiology by M.J. James, C.B.S. publishers & publishers 1987.
2. Basic food microbiology “by J.B. Gorge C.B.S. Publishers & Distributers “1987.
3. Food Microbiology by M. R. Adams and M. O. Moss
 Chhattisgarh Swami Vivekanand Technical University, Bhilai (C.G.)

Semester : IV Semester B.E Branch: Biotechnology

Subject: Stoichiometric Calculations Code: 318414(19)
Total Theory Periods: 30 Total Tut Periods: 10
Total Marks in End Semester Exam: 80
Minimum number of class test to be conducted: 2

Unit 1:- Unit and its conversion, Normality, Molality, Molarity, PVT behaviors, Gas laws, Partial
pressure and pure component volume methods. Limiting and excess reactant.
[ No. of Periods – 6+2]

Unit 2:- Humidity and Saturation: Percentage Humidity, Relative Humidity, Molal Humidity, Cox
Chart. Crystallization.
[ No. of Periods – 6+2]

Unit 3:- Combustion calculations- fuel and flue gas analysis, air fuel ratio, theoretical oxygen,
percentage excess air, limiting and excess reactant.
[ No. of Periods – 6+2]

Unit 4:- Material balance involving physical changes, chemical changes and biochemical reactions,
chemical reactions, Recycle, bypass and purge operations.
[ No. of Periods – 6+2]

Unit 5:- Energy Balance calculations: Thermo physics: Heat capacity calculations, Enthalpy
changes of chemical and biochemical reactions, Dissolution and Laws of Thermo
chemistry. Effect of pressure and temperature on heat of reactions.
[ No. of Periods – 6+2]

Name of Text Books:

1. Bhatt and Vora, ‘Stoichiometry’, McGraw Hill Publications
2. Hougen and Watson, “Chemical Process Principles Part – I Material and Energy Balance 2nd
Edition
Name of Reference Books:
1. K A Gavhane, Unit operations, Nirali publication
2. Himmelblaue, basic principles and calculations in Chemical engineering, Prentice Hall
3. Ghoshal,Sanyal,Dutta, Introduction to chemical engineering
 Chhattisgarh Swami Vivekanand Technical University, Bhilai (C.G.)

Semester : IV Semester B.E Branch: Biotechnology

Subject: Transport Processes Code: 318415(19)
Total Theory Periods: 40 Total Tut Periods: 10
Total Marks in End Semester Exam: 80
Minimum number of class test to be conducted: 2

Unit 1:- Mechanism of momentum transport: Newton’s Law of viscosity, Newtonian fluids,
pressure and temperature dependence on viscosity. Theory of viscosity of liquids. Velocity
distribution in laminar flow - Shell momentum balance, boundary conditions, flow of
falling film, flow through circular tubes and annulus, flow through rectangular duct,
adjacent flow of immiscible fluids between two parallel plates, couette flow.
[ No. of Periods – 8+2]
Unit 2:- Equations of continuity in rectangular, cylindrical and spherical coordinate, Equations of
motion, tangential annular flow and shape of cylindrical surface vortex depth.
[ No. of Periods – 8+2]
Unit 3:- Mechanism of energy transport: Fourier’s Law of heat conductions, temperature and
pressure dependence of thermal conductivity of gases and liquids. Thermal conductivity of
solids. Temperature distribution in solids and in laminar flow - Shell energy balances,
boundary conditions, heat conduction with an electrical heat source, heat conduction with
viscous heat source, heat conduction with nuclear heat source, heat conduction through
composite walls, conduction in cooling fins, forced convection and free convection.
[ No. of Periods – 8+2]
Unit 4:- Mechanism of mass transport : Fick's Law of diffusion, temperature and pressure
dependence of mass diffusivity. Theory of ordinary diffusion in gases at low density.
Theory of ordinary diffusion in liquids. Concentration distribution in solids and in laminar
flow-shell mass balance, boundary conditions, diffusion through a stagnant gas film,
Diffusion with heterogeneous chemical reaction. Diffusion into a falling liquid film.
[ No. of Periods – 8+2]
Name of Text Books:
1. Biron R. Bird, Warren E. Stewart, and Edwin Lightfoot , "Transport Phenomena".
2. Christie J. Geankoplis, "Transport Processes and Unit Operations", Prentice hall of India, 1997
Name of Reference Books:
1. Bennet C.O. and Meyer J.E., "Momentum and mass Transfer"
2. Sission and Pitts "Introduction to Transprot Phenomena"
3. J.C.Slattery, “ Momentum, Energy and Mass Transfer in continuum , Kruger Publishing
Company
 Chhattisgarh Swami Vivekanand Technical University, Bhilai (C.G.)

Semester : IV Semester B.E Branch: Biotechnology

Subject: Thermodynamics and Biochemical Code: 318416(19)
Reaction Engineering Total Tut Periods: 10
Total Theory Periods: 30
Total Marks in End Semester Exam: 80
Minimum number of class test to be conducted: 2

Unit 1:- Thermodynamic functions, Laws of thermodynamics, Heat effects-standard heat of

reaction, free energy of formation, choice Raouilts Law and Henry’s Law, Fluid phase
equilibrium.
[ No. of Periods – 6+2]
Unit 2- Chemical potential, fugacity, activity, activity coefficient, chemical reaction equilibria,
standard free energy change, equilibrium conversion, Vant Hoff’s Eq. Clausius
Clapeyron Eq: Gibbs Duhem Eq. Application of thermodynamics on Bimolecular.
[ No. of Periods – 6+2]
Unit 3:- Kinetics of homogenous reactions, reaction mechanism, temperature dependency, ate
constant, interpretation of batch kinetic data.
[ No. of Periods – 6+2]
Unit 4:- Kinetics of enzyme catalyzed reactions in free and immobilized states, choice Michaelis-
Menten equation and its various modifications. Effects of external mass transfer
immobilized enzyme systems, analysis if intraparticle diffusion and reactions.
[ No. of Periods – 6+2]
Unit 5:- Kinetics of substrate utilization, product formation and biomass production, Monod
growth model and its various modifications, structured and unstructured kinetic rate
models, thermal death rate kinetics of cells and spores.
[ No. of Periods – 6+2]
Name of Text Books:
1. Chemical Reaction Engineering : Octave Levenspiel
2. Chemical Reaction Engineering : Foggler
Name of Reference Books:
1. Chemical Engg. Thermodynamics : J.M. Smith
2. Thermodynamics : Glass Stone
3. Bio-chemical Reaction Engg: J.M. Coulson & Richarder vol-3
 Chhattisgarh Swami Vivekanand Technical University, Bhilai (C.G.)

Semester: IVth Semester B.E Branch: Biotechnology

Subject: Cellular And Molecular Biology Lab Practical Code: 318421(18)
Total Practical Periods: 30.
Total Marks in End Semester Exam: 40

Experiments to be performed (Minimum 10 experiments)

1. Study of cell division in root tip/onion bud.
2. Study of leaf structure by section cutting
3. Study of stem structure by section cutting
4. Measurement of cell size by ocular Microbiology.
5. Study of unicellular organisms in pond water.
6. Hanging drop method for studying motility of microorganisms from sewage water
7. Determination of Leukocyte Cell.
8. R.B.C. Counting.
9. W.B.C. Counting.
10. Isolation of DNA from onion Cell.
11. Study of different type of fungal and bacterial cell.

List of Equipments/Machine Required:

1. Microscope
2. Micrometer (Ocular and stage)
3. Haemocytometer
4. Ultra-centrifuge

Recommended Books:
1. Experimental Microbiology, Plant Pathology and Biotechnology by K. R. Aneja
2. Refer books mentioned in theory syllabus
 Chhattisgarh Swami Vivekanand Technical University, Bhilai (C.G.)

Semester: IVth Semester B.E Branch: Biotechnology

Subject: Recombinant DNA Technology Lab Practical Code: 318422(18)
Total Practical Periods: 30.
Total Marks in End Semester Exam: 40

Experiments to be performed (minimum 10 experiments)

1. Isolation of DNA from plant cell by CTAB method.
2. Isolation of DNA from onion cell.
3. Electrophoresis of DNA from plant/onion cell.
4. Isolation of RNA from yeast cell.
5. Estimation of DNA by Diphenyl method.
6. Estimation of RNA by orcinol method.
7. Total Genomic DNA extraction ( Kit).
8. Plasmid Conjugation ( Kit).
9. Isolation of DNA from Blood ( Kit ).
10. Silver Staining of DNA isolated from plant/Animal ( Kit).
11. DNA Ligation ( Kit).
12. Restriction Digestion (Kit).
13. Experiments to perform amplification of DNA
14. Transfer of DNA/Protein from Gel to nitrocellulose membrane by western blotting.

List of Equipments/Machine Required:

1. Refrigerated Micro-Ultracentrifuge
2. Gel Electrophoresis Apparatus
3. Oven
4. Incubator
5. UV Transilluminator
6. Micropipette
7. PCR Machine
8. Western Blotting Apparatus
9. Gel Rocker
10. –20 Refrigerator

Recommended Books:
1. Gene Cloning by T A Brown
2. Refer books mentioned in theory syllabus
 Chhattisgarh Swami Vivekanand Technical University, Bhilai (C.G.)

Semester : IV Semester B.E Branch: Biotechnology

Subject: Transport Processes Lab Practical Code: 318423(19)
Total Practical Periods: 30.
Total Marks in End Semester Exam: 40

Experiments to be performed (minimum 10 experiments)

1. To determine heat transfer coefficient of pin fin extended surfaces heat transport
2. To determine all heat transfer forces convection apparatus.
3. To determine over all heat transfer coefficient of double pipe heat exchanges.
4. To determine over all heat transfer coefficient of shell and tube heat excluder.
5. To determine coefficient of discharge of venturi and orifice.
6. To calibrate given rotameter.
7. Study of sudden expansion and sudden contraction and determine expansion and contraction
losses.
8. To determine Reynolds number of flowing fluid and draw fiction factor curve.
9. To determine discharge coefficient of weir notch.
10. To determine pressure drop in packed bed apparatus.
11. To determine fluidiction coefficient of fluidized bed.
12. To determine diffusivity in forced diffusion system.
13. Study of humidifier.
14. Study of spray dryer
15. Determine the heat transfer coefficient for heat flow through composite wall.

List of Equipments/Machine Required:

1. Pin fin Apparatus
2. Shell and tube heat exchanger
3. Sudden expansion and sudden contraction arrangement
4. Fluidized bed.
5. Spray dryer.
6. Humidifier

Recommended Books:
1. McCabe and Smith, Unit Operation of Chemical Engg
2. Coulson and Richardson, Chemical Engineering, vol 1.
3. Treyball, Mass Transfer Operation
 Chhattisgarh Swami Vivekanand Technical University, Bhilai (C.G.)

Semester : IV Semester B.E Branch: Biotechnology

Subject: Thermodynamics and Biochemical Practical Code: 318424(19)
Reaction Engineering Lab
Total Practical Periods: 30.
Total Marks in End Semester Exam: 40

Experiments to be performed (minimum 10 experiments)

1. Kinetics of Irreversible reaction in Batch Reactor
2. Kinetics of Reversible reaction in Batch Reactor
3. Kinetics of irreversible reaction in isothermal PFR .
4. Kinetics of reversible reaction in isothermal PFR .
5. Kinetics of irreversible reaction in Adiabatic Reactor.
6. Kinetics of reversible reaction in Adiabatic Reactor.
7. Kinetics of irreversible reaction in CSTR.
8. Kinetics of reversible reaction in CSTR.
9. Estimation of Carbohydrate
10. Estimation of Protein
11. Determination of Iodine value of fat
12. Determination of Saponification value of fat
13. Identification of given sample (Carbohydrate, Protein, Fat)
14. Separation of amino acid by paper chromatography.
15. Isolation of amylase producing organism
16. To determine heat of reaction for given reaction system.

List of Equipments/Machine Required:

1. Batch Reactor
2. Plug Flow Reactor (PFR)
3. Adiabatic Reactor
4. Mixed Flow Reactor (MFR)
5. Heterogeneous Catalytic Reactor
6. Biochemical Reactor
7. Photochemical Reactor
8. Spectrophotometer (UV-VIS)
9. Hot Air Oven
10. Electronic Balance Dhona
11. pH meter
Recommended Books:
1. O. Levenspiel “Chemical Reaction Engg.:”
2. H. Scott Fogler “Chemical Reaction Engg.”
3. Balleys & Ollis, Bio-Chemical Engg. Fundaments, Mc-Graw hill Publication
4. B. D. Singh, Bio- Technology, Kalyani Publication
 Chhattisgarh Swami Vivekanand Technical University, Bhilai (C.G.)
Semester : IV Sem. Branch: Common for all branches
Subject : HEALTH, HYGIENE & YOGA Code : 300425 (46)
No. of Periods : 2 pds/week Tutorial Periods : NIL
Total Marks in End Semester Exam. : NIL Teacher's Assessment: 40 Marks
Minimum number of class tests to be conducted: Two

UNIT- I
HEALTH & HYGIENE: Concept of health, Physical health and mentall health and wellbeing and how
to achieve these, longevity and how to achieve it, concept and common rules of hygiene, cleanliness and
its relation with hygiene; Overeating and undereating, amount of food intake required, intermittent
fasting; adequate physical labour, sleep; consumption of junk fast food vs nutritious food; fruits,
vegetables cereals and qualities of each of these.

UNIT- II

INTRODRCTORY KNOWLEDGE OF COMMON STREAMS OF MEDICINAL CURE: History,

development, basic concepts, modes of operation of Alopathy, Ayurved, Homoeopathy, Biochemic,
Unani, Siddha, Accurpressure, Accupunture, Naturopathy, Yogic and Herbal system of medicines,
Introduction of Anatomy and Physiology concerned.

UNIT- III
YOGASANS: Meaning and concept of Yoga, Yogasans and its mode of operation, How to perform
Yogasans, Common Yogasans with their benefits, such as, Padahastasan, Sarvangasan, Dhanurasan,
Chakrasan, Bhujangasan, Paschimottasan, Gomukhasan, Mayurasan, Matsyasan, Matsyendrasan,
Pawanmuktasan, Vajrasan, Shalabhasan, Sinhasan, Shashankasan, Surya Namaskar, Halasan,
Janushirasan, Utshep Mudra,

UNIT- IV
YOGASANS FOR COMMON DISEASES: From Yogic Materia Medica with symptoms, causes,
asans and herbal treatment.

Modern silent killers: High blood pressure, diabetes and cancer, causes and cure; Common
health problems due to stomache disorders, such as, indigestion, acidity, dycentry, piles and
fissures, artheritis, its causes, prevention and cure.

Asans for relaxation: Shavasan, Makarasan, Matsyakridasan, Shashankasan.

Asans to increase memory and blood supply to brain : Shirsh padasan, Shashankasan.

Asans for eye sight: Tratak, Neti Kriya .

Pranayam : Definition and types : Nadi Shodhan, Bhastrik, Shitakari, Bhramari useful for
students.
UNIT V
CONCENTRATION: Concentration of mind and how to achieve it. Tratak ¼=kVd½]
Concentration on breath, Japa ¼ti½] Ajapajap ¼vtikti½] internal silence ¼vUrekSZu½]
visualization in mental sky ¼fpnkdk'k /kkj.kk½] Concentration on point of light ¼T;ksfr
/;ku½] Concentration on feeling ¼Hkko /;ku½] Concentration on figure ¼ewÙkZ /;ku½-
REFERENCES
(1) Yogic Materia Medica
(2) Asan, Pranayam and Bandh
-o0o-