US01080670732

( 12 ) United States Patent ( 10) Patent No.: US 10,806,707 B2

Finley et al . (45 ) Date of Patent : Oct. 20 , 2020
( 54 ) CANNABIS OIL COMPOSITIONS AND 8,632,825 B2 1/2014 Velasco Diez et al .
METHODS FOR PREPARATION THEREOF 8,906,429 B1 12/2014 Kolsky
9,034,395 B2 5/2015 Whittle et al .
( 71 ) Applicant: Constance Therapeutics, Inc. , San 9,066,920 B2 6/2015 Whalley et al .
2003/0017213 Al 1/2003 Schmidt
Francisco, CA (US ) 2003/0134018 A1 7/2003 Turner et al .
2005/0123635 A1 6/2005 McAughey et al .
(72) Inventors : Constance Finley , San Francisco , CA 2008/0167483 A1 7/2008 Whittle et al .
(US ) ; Haley Poole Bestwick, Newberg, 2010/0166891 Al 7/2010 Schmidt
OR (US ) 2010/0249223
2011/0086113
A1
Al
9/2010
4/2011
Di Marzo et al .
Velasco Diez et al .
( 73 ) Assignee : Constance Therapeutics , Inc. , San 2012/0004251 A1 1/2012 Whalley et al .
Francisco , CA (US ) 2012/0059062 A1 3/2012 Whittle et al .
2013/0059018 Al 3/2013 Parolaro et al .
2014/0221469 Al 8/2014 Ross et al .
( * ) Notice: Subject to any disclaimer, the term of this 2014/0243405 A1 8/2014 Whalley et al .
patent is extended or adjusted under 35 2014/0271940 A1 9/2014 Wurzer
U.S.C. 154 (b ) by 79 days. 2014/0302185 A1 10/2014 Cavalieri Manasse
2014/0330030 A1 11/2014 Ferri
( 21 ) Appl . No.: 15 / 778,192 2015/0053742 A1 2/2015 Koren et al .
2015/0086653 Al 3/2015 Parolaro et al .
( 22 ) PCT Filed : Nov. 23 , 2016 2015/0105455 A1 4/2015 Bjorncrantz
2015/0105569 A1 4/2015 Emo
( 86 ) PCT No .: PCT/US2016/ 063662 2015/0136158 Al 5/2015 Stevens et al .
2015/0245991 A1 9/2015 Nihart
$ 371 ( c ) ( 1 ) , 2015/0297654 A1 10/2015 Speier
( 2 ) Date : May 22 , 2018 (Continued )
( 87 ) PCT Pub . No .: WO2017/091764 FOREIGN PATENT DOCUMENTS
PCT Pub . Date : Jun , 1 , 2017 CA 2582289 10/2014
CN 103005427 A 4/2013
( 65 ) Prior Publication Data CN 104304815 A 1/2015
US 2018/0344661 A1 Dec. 6 , 2018 (Continued )
Related U.S. Application Data OTHER PUBLICATIONS
( 60 ) Provisional application No. 62/ 259,549 , filed on Nov. Cook - Mills , J.M. , et al . , “ Two faces of vitamin E in the lung .” Am .
24 , 2015 . J. Respir. Crit . Care Med . 188 , 279-284 , 2013 .
(Continued )
( 51 ) Int . Cl .
A61K 36/00 ( 2006.01 )
A61K 31/05 ( 2006.01 ) Primary Examiner Michael V Meller
A61K 31/192 ( 2006.01 ) (74 ) Attorney, Agent, or Firm — Kilpatrick Townsend &
A61K 312355 ( 2006.01 )
A61K 36/185 ( 2006.01) Stockton LLP
A61K 31/352 ( 2006.01 )
A61P 35/00 ( 2006.01 ) ( 57 ) ABSTRACT
( 52 ) U.S. CI .
CPC A61K 31/05 ( 2013.01 ) ; A61K 31/192 The present invention provides cannabis oil compositions,
(2013.01 ) ; A61K 31/352 ( 2013.01 ) ; A61K including cannabis oil compositions containing vitamin E ,
31/355 (2013.01 ) ; A61K 36/185 ( 2013.01 ) ; and methods for preparing the cannabis oil compositions. In
A61P 35/00 ( 2018.01 ) ; A61K 2300/00 some embodiments, the invention provides a method for
(2013.01 ) preparing a cannabis oil composition comprising eluting
( 58 ) Field of Classification Search cannabinoids from cannabis plant material with a solvent to
CPC A61K 36/00 produce an eluate; filtering the eluate with a filter to produce
USPC 424/725
See application file for complete search history. a filtrate; evaporating the solvent from the filtrate with a
distiller to produce a distillate; and purging the distillate
( 56 ) References Cited under conditions sufficient to remove residual solvent. In
some embodiments, the method further includes mixing a
U.S. PATENT DOCUMENTS quantity of vitamin E with the extract. Blended cannabis oil
6,079,418 A 6/2000 Russo
compositions containing mixtures of cannabis oil prepara
6,383,513 B1 5/2002 Watts et al . tions are also described .
7,344,736 B2 3/2008 Whittle et al .
7,887,857 B1 2/2011 Johnson
8,445,034 B1 5/2013 Coles , Jr. 4 Claims , 16 Drawing Sheets
 US 10,806,707 B2
Page 2

( 56 ) References Cited Stark , Michael , “ Marijuana Chemistry Genetics, Processing &

Potency ” , Sep. 1 , 1993 , URL : https://catnews.org/FREE%20Pot%
U.S. PATENT DOCUMENTS 20Books/Marijuana % 20Chemistry % 20- % 20Genetics, % 20Processing , %
2015/0313867 Al 11/2015 Velasco Diez et al.
20 & % 20Potency % 20- % 20Michael% 20S tarks.pdf, retrieved Aug.
1 , 2018, 206 pages.
N.N .: " Hash Oil ” , Aug. 1 , 2018 , URL : https://en.wikipedia.org/
FOREIGN PATENT DOCUMENTS wild / Hash_oil, retrieved Aug. 1 , 2018 , 5 pages .
GB 2 439 393 A 12/2007
Supplementary European Search Report, dated Sep. 24 , 2019 , 4
pages.
WO 99/32107 A1 7/1999
WO 2012/071389 A2 5/2012 Berardi, J. , “ All About Hemp,” Precision Nutrition, 20 pgs . , 2018 .
WO 2013/057487 Al 4/2013 Leizer, C. et al . , “ The Composition of Hemp Seed Oil and Its
WO 2013/068744 A2 5/2013 Potential as an Important Source ofNutrition ,” Journal of Nutraceuticals,
WO 2013/165251 Al 11/2013 vol . 2 ( 4 ) : 35-53 , 2000 .
International Search Report, dated Feb. 7 , 2017 for International
OTHER PUBLICATIONS Pat. Appl. No. PCT /US2016 /063662, 3 pages .
Mansouri, H. et al . , “ Effects of Gibberellic Acid on Primary
Hamahata , A. et al . , “ y - Tocopherol nebulization by a lipid aerosoliza Terpenoids and 19 — Tetrahydrocannabinol in Cannabis sativa at
Flowering Stage,” Journal of Integrative Plant Biology, vol . 51 ( 6 ) :
tion device improves pulmonary function in sheep with burn and 553-561 , 2009 .
smoke inhalation injury .” Free Radical Biology and Medicine 45 , Medical Jane, Cannabis Extraction : learn about the various methods
425-433 , 2008 . in which cannabis is extracted [ online ] , 2016 , [ retrieved on Nov. 1 ,
Hybertson, B.M. et al . , “ Aerosol -administered alpha - tocopherol 2016 ] . Retrieved from the Internet: < URL : http ://www.medicaljane.
attenuates lung inflammation in rats given lipopolysaccharide intratrache com /category /cannabis - classroom /extractions-methods/ > , 7 pages .
ally . ” Exp . Lung Res. 31 , 283-294 , 2005 . Mediwiet , HomeStichting Mediwiet [ online ] , 2016 , [ retrieved on
Morita , N. , et al . “ Aerosolized alpha - tocopherol ameliorates acute Nov. 1 , 2016 ] . Retrieved from the Internet: < URL : http ://www .
lung injury following combined burn and smoke inhalation injury in stichtingmediwiet.nl/ > , 1 page .
sheep .” Shock 25 , 277 , 2006 . Romano, L. and Hazekamp, A. , “ Cannabis Oil : chemical evaluation
Rizvi, S. , et al . , “ The Role of Vitamin E in Human Health and Some of an upcoming cannabis -based medicine,” Cannabinoids, 1(1 ):1
Diseases . ” Sultan Qaboos Univ Med J 14 , el 57 - e165 , 2014 . 11 , 2013
Yamamoto , Y. et al . , " Nebulization with Gamma - Tocopherol Ame The Chill Bud “ 15 Cannabis Strains High in Cannabidiol (CBD ) ”
liorates Acute Lung Injury after Burn and Smoke Inhalation in the Aug. 2015 [ online ] <URL : http://thechillbud.com/15-cannabis
Ovine Model ” Shock 37 , 408-414 , 2012 . strains-high - in - cannabidiol-cbd/ > .
U.S. Patent Oct. 20 , 2020 Sheet 1 of 16 US 10,806,707 B2

100

STEP 102
FREEZE CANNABIS PLANT MATERIAL 103
AND SOLVENT 105
STEP 104
ELUATE CANNABINOIDS 107 TO PRODUCE
ELUATE 109

FIRST ELUATE SECOND ELUATE

111 113
STEP 106

SOLARIZE ELUATE
STEP 108

FILTER ELUATE 109 TO PRODUCE FILTRATE 115

STEP 110
EVAPORATE SOLVENT 105 FROM FILTRATE 115 TO
PRODUCE DISTILLATE 117
STEP 112
DEHYDRATE DISTILLATE 117 TO PRODUCE
EXTRACT 119
STEP 114

MIX VITAMIN E 121 WITH EXTRACT 119

FIG . 1A
U.S. Patent Oct. 20 , 2020
9 Sheet 2 of 16 US 10,806,707 B2
100
STEP 102
FREEZE CANNABIS PLANT MATERIAL 103
AND SOLVENT 105
STEP 104
ELUATE CANNABINOIDS 107 TO PRODUCE
ELUATE 109

FIRST ELUATE SECOND ELUATE

111 113
STEP 106
|
1 SOLARIZE ELUATE 1
I
I STEP 108

FILTER ELUATE 109 TO PRODUCE FILTRATE 115

STEP 110
EVAPORATE SOLVENT 105 FROM FILTRATE 115 TO
PRODUCE DISTILLATE 117
STEP 110a
HEAT DISTILLATE 117 TO FORM
DECARBOXYLATED DISTILLATE 117a
STEP 110b
FILTER DECARBOXYLATED DISTILLATE 117a TO
FORM DECARBOXYLATED FILTRATE 117b
STEP 112a
PURGE DECARBOXYLATED FILTRATE 117b TO
PRODUCE DECARBOXYLATED EXTRACT 119b
STEP 114a
MIX VITAMIN E 121 WITH DECARBOXYLATED
EXTRACT 1195

FIG . 1B
U.S. Patent Oct. 20 , 2020
9 Sheet 3 of 16 US 10,806,707 B2

VISCOSITY OF CANNABIS OIL COMPOSITION vs VITAMIN E

PERCENTAGE
7000
6000
5000
VCP
(
I)SCOSITY 4000
3000
2000
1000
0
10 20 30 40 50
VITAMIN E PERCENTAGE ( %)

FIG . 2
U.S. Patent Oct. 20, 2020 Sheet 4 of 16 US 10,806,707 B2

THC AND CBD PERCENTAGES IN CANNABIS OIL COMPOSITIONS

MADE FROM DIFFERENT STRAINS OF CANNABIS PLANT MATERIAL

70.00

60.00

50.00
PC(%)AENRACBEINOITD 40.00 % THC
% CBD
30.00
20.00

10.00

0.00

(NOVITAMINE) (NOVITAMINE)
EXTRACMADET FROMFROMACDCACDCWITH30EX%TWR/WAVITAMIN WITHOUTVITAMIEN
CMADET FROMEBLUEBERRY
BLUEBERWITHY 30%W/WVITAMINE CANNATONICWITH30%W/WVITAMINE
CAN ABIOILSCOMPOSITIMADEON CAN ABOILISCOMPOSITIEMADEOXNTRFROMACMADET CAFROMN CBAOILINSCATMOPNOISCITIMADEON FROM

FIG . 3
U.S. Patent Oct. 20 , 2020
9 Sheet 5 of 16 US 10,806,707 B2
SF - 268
150

100
Growth
%
() 50 Blended Oil 20
+ Blended Oil 30
0 Oil 50
0 1 3 10 * Blended Oil 40
* Oil 60
-50
-100
Extract ( uM )
FIG . 4A

SF - 268
120
100
80
60
40 - CBD : THC mix 20
Growth
%
(
) 20 + CBD : THC mix 30
CBD : THC mix 50
0 CBD : THC mix 40
-20 0 1 3 10 30 CBD : THC mix 60
-40
-60
-80
Extract (UM )
FIG . 4B
SF - 268
120
100
80
60
Growth
(
%
) 40 Delta8 THC
20 + Delta9 THC
0 CBD
-20
0 3 10 30 11Hy delta9THC
-40
-60
-80
Extract (uM)

FIG . 4C
U.S. Patent Oct. 20 , 2020 Sheet 6 of 16 US 10,806,707 B2
SF - 295
150

100
Growth
%
(
) 50 - Blended Oil 20
Blended Oil 30
Oil 50
0 # Blended Oil 40
0 1 3 10 Oil 60
-50

-100
Extract ( UM )
FIG . 5A
SF - 295
120
100
80
60
Growth
(
%
) 40 CBD : THC mix 20
+ CBD : THC mix 30
20 CBD : THC mix 50
0 + CBD : THC mix 40
-20 0 1 3 10 * CBD : THC mix 60
-40
-60
-80
Extract ( UM)
FIG . 5B

SF -295
120
HH
H

100
80
60
Growth
() 40
% Delta8 THC
20 + Delta9 THC
0 CBD
-20 0 3 10 30 11Hy delta9THC
-40
-60
-80
Extract (uM)
FIG . 5C
U.S. Patent Oct. 20 , 2020
9 Sheet 7 of 16 US 10,806,707 B2
SF - 539
150
100
50 Blended Oil 20
Growth
%
(
) 0 # Blended Oil 30
0 1 3 10 30 Oil 50
* Blended Oil 40
-50 * Oil 60
-100
-150
Extract (uM)
FIG . 6A
SF - 539
120
100
80
60
Growth
(
%
) 40 CBD : THC mix 20
20 CBD : THC mix 30
CBD : THC mix 50
0 * CBD : THC mix 40
0 1 3 10 30 * CBD : THC mix 60
-20
-40
-60
-80
Extract (UM )
FIG . 6B

SF - 539
120
100
80
60
Growth
(
%
) 40 Delta8 THC
20 + Delta9 THC
0 CBD
-20
0 3 10 30 * 11 Hy delta9THC
-40
-60
-80
Extract (uM)
FIG . 6C
U.S. Patent Oct. 20 , 2020
9 Sheet 8 of 16 US 10,806,707 B2
SNB - 19
150
100
Blended Oil 20
) 50
Growth
(
% Blended Oil 30
Oil 50
0 30 * Blended Oil 40
0 1 3 10 me Oil 60
-50
-100
Extract (um)
FIG . 7A

SNB - 19
120
100
80
) 60
Growth
%
( 40 CBD : THC mix 20
20 CBD : THC mix 30
0
CBD : THC mix 50
0 1 3 10 30 + CBD : THC mix 40
-20 CBD : THC mix 60
-40
-60
-80
Extract (um)
FIG . 7B

SNB - 19
120
100
80
60
Growth
(
) 40
% Delta8 THC
20 Delta 9 THC
0 CBD
-20
0 3 10 11Hy delta9THC
-40
-60
-80
Extract (uM)
FIG , 70
U.S. Patent Oct. 20 , 2020
9 Sheet 9 of 16 US 10,806,707 B2
SNB- 75
150
100
Blended Oil 20
) 50
Growth
%
( Blended Oil 30
Oil 50
0 Blended Oil 40
0 1 3 10 * Oil 60
-50
-100 Extract (UM)
FIG . 8A
SNB - 75
120
100
80
??
60
CBD : THC mix 20
%
() 40
Growth CBD : THC mix 30
20 CBD : THC mix 50
0 * CBD : THC mix 40
-20
0 1 3 10 CBD : THC mix 60
-40
-60
-80
Extract (uM)
FIG . 8B

SNB - 75
120
100
80
60
Growth
(
%
) 40 + Delta8 THC
20 Delta9 THC
0 CBD
-20
0 3 10 30 11Hy delta9THC
-40
-60
-80
Extract (MM)
FIG . 8C
U.S. Patent Oct. 20 , 2020 Sheet 10 of 16 US 10,806,707 B2
U - 251
150
100
Growth
%
(
) 50 Blended Oil 20
Blended Oil 30
Oil 50
0 Blended Oil 40
0 1 3 10 Oil 60
-50
-100
Extract (uM)
FIG . IA
U - 251
120
100
80
60
CBD : THC mix 20
%
() 40
Growth + CBD : THC mix 30
20 CBD : THC mix 50
0
0 1 3 10 30 + CBD : THC mix 40
-20 CBD : THC mix 60
-40
-60
-80
Extract (UM)
FIG . 9B
U - 251
120
100
80
60
) 40
Growth
(
% the Delta8 THC
20 mother Delta9 THC
0 » CBD
-20 0 3 10 11Hy delta9THC
-40
-60
-80
Extract (uM )
FIG . 9C
U.S. Patent Oct. 20 , 2020
9 Sheet 11 of 16 US 10,806,707 B2
MCF - 7
150

100

50
Blended Oil 20
Growth
(
)
% + Blended Oil 30
Oil 50
0 Blended Oil 40
0 1 3 10 Oil 60
-50
-100
Extract (uM)
FIG . 10A
MCF - 7
120
100
80
60
Growth
(
%
) 40 CBD : THC mix 20
20 CBD : THC mix 30
CBD : THC mix 50
0 * CBD : THC mix 40
0 1 3 10 30
-20 * CBD : THC mix 60
-40
-60
-80
Extract (um)
FIG . 10B
MCF - 7
120
100 #
Hi
80
60
Growth
(%
) 40 Delta8 THC
20 Delta9 THC
0 30
CBD
-20
3 10 * 11Hy delta9THC
-40
-60
-80
Extract (um )
FIG . 10C
U.S. Patent Oct. 20 , 2020 Sheet 12 of 16 US 10,806,707 B2
BT549
150

100
50 Blended Oil 20
Growth
%
(
) Blended Oil 30
Oil 50
0 * Blended Oil 40
0 1 3 30 Oil 60
-50
-100
Extract (um)
FIG , 11A
BT549
120
100
80
60
Growth
(
%
) 40 CBD : THC mix 20
20 CBD : THC mix 30
nye CBD : THC mix 50
0 0 1 3 10 130 + CBD : THC mix 40
-20 CBD : THC mix 60
-40
-60
-80
Extract (um)
FIG . 11B
BT549
120
100
80
60
Growth
(
%
) 40 Delta8 THC
20 Delta9 THC
0 CBD
-20
0 3 10 * 11Hy delta9THC
-40
-60
-80
Extract (uM)
FIG . 110
U.S. Patent Oct. 20 , 2020
9 Sheet 13 of 16 US 10,806,707 B2
MDA - MB231
150

100
Growth
() 50
% Blended Oil 20
• Blended Oil 30
Oil 50
0 Blended Oil 40
0 1 3 10 30
Oil 60
-50
-100 Extract (um)
FIG . 12A
MDA - MB231
120
100
80
60
Growth
%
(
) 40 CBD : THC mix 20
+ CBD : THC mix 30
20 CBD : THC mix 50
0 + CBD : THC mix 40
0 1 3 10 30 CBD : THC mix 60
-20
-40
-60
Extract (um)
FIG . 12B

MDA -MB231
120
100
80
60
Growth
()
% 40 Delta8 THC
20 + Delta9 THC
0 CBD
-20
0 3 10 30 * 11 Hy delta9THC
-40
-60
-80
Extract (uM)

FIG . 12C
U.S. Patent Oct. 20 , 2020
9 Sheet 14 of 16 US 10,806,707 B2
MDA --MB468
150
100
Growth
() 50
% Blended Oil 20
Blended Oil 30
Oil 50
0 0 1 Blended Oil 40
3 10
na Oil 60
-50
-100
Extract (uM )
FIG . 13A

MDA- MB468
120
100
80
60
Growth
%
(
) 40 CBD : THC mix 20
CBD : THC mix 30
20 CBD : THC mix 50
0 30 + CBD : THC mix 40
-20 0 1 3 10 CBD : THC mix 60
-40
-60
-80
Extract (uM )
FIG . 13B

MDA -MB468
120
100
80
60
Growth
%
(
) 40 Delta8 THC
20 + Delta9 THC
0 CBD
-20 0 3 10 30 11Hy delta9THC
-40
-60
-80
Extract (UM)
FIG . 13C
U.S. Patent Oct. 20 , 2020 Sheet 15 of 16 US 10,806,707 B2
T - 47D
150

100
?
1

Growth
(
%
) 50 Blended Oil 20
Blended Oil 30
- Oil 50
0 Blended Oil 40
0 1 3 30
Oil 60
-50
-100
Extract(UM )
FIG . 14A
T47D
120
100 H?
80
*
60
Growth
%
(
) 40 CBD : THC mix 20
20 CBD : THC mix 30
CBD : THC mix 50
0 * CBD : THC mix 40
-20 0 1 3 10 30 CBD : THC mix 60
-40
-60
-80
Extract (uM )
FIG . 14B

T47D
120
100
80
60
Growth
%
(
) 40 + Delta8 THC
20 Delta9 THC
0 CBD
0 3 10 30 11Hydelta9THC
-20
-40
-60
-80
Extract (UM)

FIG . 14C
U.S. Patent Oct. 20 , 2020
9 Sheet 16 of 16 US 10,806,707 B2
H5578T
150

100
Growth
(
%
) 50 Blended Oil 20
+ - Blended Oil 30
ve Oil 50
0 * Blended Oil 40
0 1 3 10 Oil 60
-50
-100
Extract (uM )
FIG . 15A
H5578T
120
100
80
60
Growth
%
() 40 CBD : THC mix 20
20 + CBD : THC mix 30
CBD : THC mix 50
0 * CBD : THC mix 40
-20 0 1 3 10 30 San CBD : THC mix 60
-40
-60
-80
Extract (um)
FIG . 15B
H5578T
120
100
80
60
Growth
%
(
) 40 + Delta8 THC
20 Delta9 THC
0 CBD
0 3 10 30 11Hy delta9THC
-20
-40
-60
-80
Extract (PM)
FIG . 15C
 US 10,806,707 B2
1 2
CANNABIS OIL COMPOSITIONS AND the composition to less than 3500 cP. In some embodiments,
METHODS FOR PREPARATION THEREOF the method includes eluting cannabinoids and terpenes from
cannabis plant material to produce the eluate . In some
CROSS - REFERENCES TO RELATED embodiments, the method includes mixing the extract with
APPLICATIONS 5 essential oils .
In certain embodiments, the invention provides a compo
The present application is a U.S. national phase of PCT sition comprising a first cannabis oil preparation having a
Application No. PCT/US2016/ 063662 , filed on Nov. 23 , first cannabinoid profile and a second cannabis oil prepa
2016 , which claims priority to U.S. Provisional Pat . Appl. 10
ration having a second cannabinoid profile . In some embodi
No. 62 /259,549 , filed Nov. 24 , 2015 , which application is ments, the composition comprises 1 ° -tetrahydrocannabinol
incorporated herein by reference in its entirety for all pur (THC ) and ( - ) -cannabidiol (CBD ) . In some embodiments ,
poses . the ratio of THC to CBD ranges from about 20 : 1 to about
FIELD OF TECHNOLOGY 1:20 by weight. In some embodiments, the ratio of THC to
15 CBD is about 4 : 1 by weight. In some embodiments, the
composition comprises one or more additional cannabis oils
This disclosure relates generally to cannabis oils and having
cannabis oil formulations, including cannabis oil composi additional cannabinoid profiles.
tions with vitamin E , and methods of preparation thereof. The methods and compositions disclosed herein may be
implemented in any means for achieving various aspects .
BACKGROUND OF THE INVENTION 20 Other features will be apparent from the accompanying
drawings and from the detailed description that follows.
The medicinal use of oils and extracts derived from
cannabis plant material has been growing in popularity. For BRIEF DESCRIPTION OF THE DRAWINGS
example , pharmacologically active compounds in cannabis
plant material including, but not limited to , delta - 9 -tetrahy- 25 Exemplary embodiments are illustrated by way of
drocannabinol (or THC ) and cannabidiol (CBD ) have been example and are not limited to the figures of the accompa
shown to reduce the effects of nausea and vomiting caused nying drawings, in which , like references indicate similar
by certain chemotherapy treatments. Research has also elements .
shown the ability of cannabinoids and other compounds FIG . 1A shows a method of preparing a cannabis oil
found in cannabis to stimulate bone growth , relieve pain, aid 30 composition according to one embodiment of the invention .
sleep , inhibit bacterial cell growth , inhibit cancer cell FIG . 1B shows a method of preparing a cannabis oil
growth , and alleviate or otherwise reduce the symptoms of composition according to an embodiment of the invention
cancer, epilepsy, autoimmune disease , neurodegeneration, including optional decarboxylation and filtration steps .
Alzheimer's disease , Lyme disease , post -traumatic stress
disorder, and inflammation . Furthermore, extracts of canna- 35 oilFIG . 2 shows a graph depicting the viscosities of cannabis
compositions as a function of vitamin E percentages in
bis plant material, whether ingested or inhaled, have also the cannabis oil compositions .
been shown to have therapeutic effects in patients with FIG . 3 is a graph depicting THC and CBD percentages in
glaucoma , dysmenorrhea , migraines , anxiety disorders, or a cannabis oil compositions made from various strains of
combination thereof.
However, cannabis oil is often highly viscous, making it 40 cannabis plant material.
difficult to work with and load into new delivery devices theFIG . 4A shows the growth of SF - 268 brain cancer cells in
such as vaporizers and E - cigarettes. In addition , such oils , singlepresence of blended cannabis oil compositions and
- strain cannabis oil compositions.
when vaporized or smoked, are often rough on a patient's
throat and may induce coughing or gagging . FIG . 4B shows the growth of SF - 268 brain cancer cells in
Therefore , a solution is needed in order to make such 45 the presence THC and CBD at concentrations equal to the
extracts more conducive to today's delivery devices and concentration in blended cannabis oil compositions and
make the inhalation / consumption of such extracts more single - strain cannabis oil compositions.
palatable for patients. In addition, such a solution should FIG . 4C shows the growth of SF - 268 brain cancer cells in
also not have an adverse effect on the potency of the the presence of isolated cannabinoids .
extract's active compounds and preserve the extract's gus 50 FIG . 5A shows the growth of SF - 295 brain cancer cells in
tatory or aromatic qualities . the presence of blended cannabis oil compositions and
single - strain cannabis oil compositions.
BRIEF SUMMARY OF THE INVENTION FIG . 5B shows the growth of SF - 295 brain cancer cells in
the presence THC and CBD at concentrations equal to the
Disclosed herein are cannabis oil extracts, compositions 55 concentration in blended cannabis oil compositions and
containing blended mixtures of the extracts, and methods for single - strain cannabis oil compositions.
preparing the extracts and compositions. In particular, a FIG . 5C shows the growth of SF - 295 brain cancer cells in
method is disclosed for preparing a cannabis oil composi the presence of isolated cannabinoids.
tion comprising eluting cannabinoids from cannabis plant FIG . 6A shows the growth of SF - 539 brain cancer cells in
material with a solvent to produce an eluate, filtering the 60 the presence of blended cannabis oil compositions and
eluate with a filter to produce a filtrate, evaporating the single -strain cannabis oil compositions.
solvent from the filtrate with a distiller to produce a distil FIG . 6B shows the growth of SF - 539 brain cancer cells in
late , and dehydrating /purging the distillate with a dehydrator the presence THC and CBD at concentrations equal to the
or vacuum oven to produce an extract . In some embodi concentration in blended cannabis oil compositions and
ments, the method further includes mixing a quantity of 65 single - strain cannabis oil compositions.
vitamin E with the extract. In some embodiments , the FIG . 6C shows the growth of SF - 539 brain cancer cells in
quantity of vitamin E is sufficient to reduce the viscosity of the presence of isolated cannabinoids .
 US 10,806,707 B2
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FIG . 7A shows the growth of SNB - 19 brain cancer cells FIG . 14B shows the growth of T -47D breast cancer cells
in the presence of blended cannabis oil compositions and in the presence THC and CBD at concentrations equal to the
single - strain cannabis oil compositions. concentration in blended cannabis oil compositions and
FIG . 7B shows the growth of SNB - 19 brain cancer cells single - strain cannabis oil compositions.
in the presence THC and CBD at concentrations equal to the 5 FIG . 14C shows the growth of T -47D breast cancer cells
concentration in blended cannabis oil compositions and in the presence of isolated cannabinoids.
single - strain cannabis oil compositions . FIG . 15A shows the growth of HS578T breast cancer cells
FIG . 7C shows the growth of SNB - 19 brain cancer cells in the presence of blended cannabis oil compositions and
in the presence of isolated cannabinoids. single - strain cannabis oil compositions.
FIG . 8A shows the growth of SNB - 75 brain cancer cells 10 FIG . 15B shows the growth of HS578T breast cancer cells
in the presence of blended cannabis oil compositions and in the presence THC and CBD at concentrations equal to the
single - strain cannabis oil compositions. concentration in blended cannabis oil compositions and
FIG . 8B shows the growth of SNB - 75 brain cancer cells single - strain cannabis oil compositions.
in the presence THC and CBD at concentrations equal to the 15 FIG . 15C shows the growth of HS578T breast cancer cells
concentration in blended cannabis oil compositions and in the presence of isolated cannabinoids.
single - strain cannabis oil compositions. Other features of the present embodiments will be appar
FIG . 8C shows the growth of SNB - 75 brain cancer cells ent from the accompanying drawings and from the detailed
in the presence of isolated cannabinoids. description that follows.
FIG . 9A shows the growth of U251 brain cancer cells in 20
the presence of blended cannabis oil compositions and DETAILED DESCRIPTION OF THE
single - strain cannabis oil compositions. INVENTION
FIG . 9B shows the growth of U251 brain cancer cells in
the presence THC and CBD at concentrations equal to the I. Definitions
concentration in blended cannabis oil compositions and 25
single - strain cannabis oil compositions. Unless specifically indicated otherwise , all technical and
FIG . 9C shows the growth of U251 brain cancer cells in scientific terms used herein have the same meaning as
the presence of isolated cannabinoids . commonly understood by those of ordinary skill in the art to
FIG . 10A shows the growth of MCF - 7 breast cancer cells which this invention belongs . In addition, any method or
in the presence of blended cannabis oil compositions and 30 material similar or equivalent to a method or material
single - strain cannabis oil compositions. described herein can be used in the practice of the present
FIG . 10B shows the growth of MCF - 7 breast cancer cells invention. For purposes of the present invention , the fol
in the presence THC and CBD at concentrations equal the lowing terms are defined .
concentration in blended cannabis oil compositions and The term “ cannabis ” refers to plants of the genus canna
single - strain cannabis oil compositions. 35 bis, including cannabis sativa , cannabis indica , and canna
FIG . 10C shows the growth of MCF - 7 breast cancer cells bis ruderalis.
in the presence of isolated cannabinoids. The term “ cannabis oil ” refers to a mixture of compounds
FIG . 11A shows the growth of BT549 breast cancer cells obtained from the extraction of cannabis plants . Such com
in the presence of blended cannabis oil compositions and pounds include , but are not limited to , cannabinoids , ter
single - strain cannabis oil compositions . 40 penes, terpenoids, and other compounds found in the can
FIG . 11B shows the growth of BT549 breast cancer cells nabis plant. The exact composition of cannabis oil will
in the presence THC and CBD at concentrations equal to the depend on the strain of cannabis that is used for extraction ,
concentration in blended cannabis oil compositions and the efficiency and process of the extraction itself, and any
single - strain cannabis oil compositions. additives that might be incorporated to alter the palatability
FIG . 11C shows the growth of BT549 breast cancer cells 45 or improve administration of the cannabis oil .
in the presence of isolated cannabinoids. The term “ cannabinoid” refers to a chemical compound
FIG . 12A shows the growth of MDA - MB231 breast that shows direct or indirect activity at a cannabinoid
cancer cells in the presence of blended cannabis oil com receptor. There are two main cannabinoid receptors , CNR1
positions and single -strain cannabis oil compositions. (also known as CB1 ) and CNR2 (also known as CB2 ) . Other
FIG . 12B shows the growth of MDA - MB231 breast 50 receptors that research suggests have cannabinoid activity
cancer cells in the presence THC and CBD at concentrations include the GPR55 , GPR18 , and TRPV1 receptors. The term
equal to the concentration in blended cannabis oil compo “ phytocannabinoid ” refers to cannabinoids that occur in a
sitions and single - strain cannabis oil compositions. plant species or are derived from cannabinoids occurring in
FIG . 12C shows the growth of MDA - MB231 breast a plant species . Examples of cannabinoids include, but are
cancer cells in the presence of isolated cannabinoids. 55 not limited to , tetrahydrocannabinol (THC ) , cannabidiol
FIG . 13A shows the growth of MDA - MB468 breast ( CBD ) , cannabinol ( CBN ) , cannabigerol ( CBG ) , cannabi
cancer cells in the presence of blended cannabis oil com chromene (CBC ) , cannabicyclol (CBL ) , cannabivarin
positions and single - strain cannabis oil compositions. ( CBV ) , tetrahydrocannabivarin ( THCV ) , cannabidivarin
FIG . 13B shows the growth of MDA - MB468 breast (CBDV) , cannabichromevarin (CBCV) , cannabigerovarin
cancer cells in the presence THC and CBD at concentrations 60 (CBGV), and cannabigerol monomethyl ether (CBGM) .
equal to the concentration in blended cannabis oil compo The term “ acidic cannabinoid ” refers to a cannabinoid
sitions and single - strain cannabis oil compositions. having one or more carboxylic acid functional groups .
FIG . 13C shows the growth of MDA - MB468 breast Examples of acidic cannabinoids include, but are not limited
cancer cells in the presence of isolated cannabinoids . to , tetrahydrocannabinolic acid ( THCA ), cannabidiolic acid
FIG . 14A shows the growth of T -47D breast cancer cells 65 (CBDA) , and cannabichromenic acid ( CBC ) . Acidic can
in the presence of blended cannabis oil compositions and nabinoids are frequently the predominant cannabinoids
single - strain cannabis oil compositions. found in raw (i.e. , unprocessed ) cannabis plant material.
 US 10,806,707 B2
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The term “ neutral cannabinoid ” refers to a cannabinoid The term “ viscosity ” is used to quantify the resistance of
without carboxylic acid functional groups. Examples of a substance such as a cannabis oil to deformation under
neutral cannabinoids include , but are not limited to , THC , shear stress and / or tensile stress .
CBD , CBG , CBC , and CBN . The term “ essential oil ” refers to natural plant oil typically
As used herein , the term “ cannabinoid profile ” refers to 5 obtained by distillation and having a chemical composition
the number, identity, and quantity of cannabinoids in a and organoleptic properties (e.g. , fragrance ) characteristic of
cannabis oil preparation. Cannabis oil compositions of the the plant or other source from which it is extracted .
invention generally contain a blend of at least two cannabis The term “ strain ” refers to different varieties of a particu
oil preparations having distinct cannabinoid profiles. In lar plant genus . For example, the term strain can refer to
certain embodiments, cannabis oil preparations are blended 10 different varieties of cannabis plants. Different cannabis
strains often exhibit distinct chemical compositions with
to provide defined cannabinoid levels in the compositions. characteristic levels of cannabinoids and terpenes, as well as
When a certain amount ( e.g. , 75 % ) of a particular cannabi other components
noid ( e.g. , THC ) is derived from a particular cannabis oil files associated with. Differing cannabinoid and terpene pro
different cannabis strains can be useful
preparation (e.g. , a first cannabis oil preparation ), that 15 for the treatment of different diseases , or for treating differ
amount of the cannabinoid is said to be “ present” in the
cannabinoid profile corresponding to the cannabis oil prepa entThe subjects with the same disease .
term “ vitamin E ” refers to a group of compounds that
ration . When 75 % of the THC in a composition is said to be include both tocopherols and tocotrienols including, but not
present in a first cannabinoid profile, for example, it is meant limited to a -tocopherol, B -tocopherol, y - tocopherol, 8 - to
that 75 % of the THC in the final composition is derived from 20 copherol, a - tocotrienol, B - tocotrienol, y - tocotrienol, d - to
a first cannabis oil preparation in the composition. The cotrienol, salts thereof, and combinations thereof. Vitamin E
remaining 25 % of the THC in the final composition is can be obtained from sources including , but not limited , to
derived from the other cannabis oil preparations (e.g. , a soybeans , sunflowers, and combinations thereof.
second or third cannabis oil preparation ) in the blend . It will The terms “ treat , ” “ treating ,” and “ treatment ” refer to any
be appreciated that cannabinoid profiles do not remain 25 indicia of success in the treatment or amelioration of an
distinct from each other, or otherwise segregated, after injury, pathology , condition , or symptom ( e.g. , pain ) , includ
blending in a composition . ing any objective or subjective parameter such as abatement;
The term “ degradation ” refers to the structural and / or remission ; diminishing of symptoms or making the symp
chemical deterioration of a substance such as chlorophyll or tom , injury , pathology or condition more tolerable to the
other plant components. Degradation can include, for 30 patient; decreasing the frequency or duration of the symp
example, the alteration of chemical structure , oxidation tom or condition ; or, in some situations, preventing the onset
state , or metal-binding properties of the substance . of the symptom . The treatment or amelioration of symptoms
The term “ eluate ” refers to a solution that is collected after can be based on any objective or subjective parameter;
contacting a plant material, such as raw cannabis plant including, e.g. , the result of a physical examination .
material, with an extraction solvent. The eluate can contain 35 The terms “ a,” “ an ,” or “ the” as used herein include plural
dissolved cannabinoids as well as other compounds of referents unless the context clearly dictates otherwise .
medicinal value . The terms “ about” and “ around , ” as used herein to modify
The term " solarizing ” refers to exposing an eluate to a a numerical value , indicate a close range surrounding that
light source . Solarizing can be achieved using natural or explicit value . If “ X ” were the value , " about X ” or “ around
non -natural light sources. In some instances, the light source 40 X ” would indicate a value from 0.8x to 1.2x , preferably a
is used to improve the quality and / or palatability of the value from 0.9x to 1.1x , and, more preferably, a value from
eluate . 0.95x to 1.05x . Any reference to “ about X ” or “ around X ”
The term “ filtrate ” refers to a solution that has passed specifically indicates at least the values X , 0.95x , 0.96x ,
through a membrane or strainer of variable porousness or 0.97 % , 0.98x , 0.99x , 1.01x , 1.02x , 1.03x , 1.04x , and 1.05x .
permeability to remove either particulate matter or unwanted 45 Thus, “ about X ” and “ around X ” are intended to teach and
compounds. In the methods of the invention , an eluate is provide written description support for a claim limitation of,
passed through a filter to produce a filtrate . e.g. , " 0.98x .”
The term “ distillate ” refers to a solution that has been
concentrated by any known means of evaporation or distil II . Extraction Methods for Preparing Cannabis Oils
lation . In the methods of the invention , the filtrate is evapo- 50
rated to form the distillate . In one aspect , the present invention provides methods for
The terms “ dehydration ” and “ dehydrating ” refer to a preparing cannabis extracts and compositions containing the
process of purging or otherwise removing residual solvent extracts . The method includes eluting cannabinoids from
from the distillate . In the methods of the invention, the cannabis plant material with a solvent to produce an eluate ,
distillate can be dehydrated by methods including use of a 55 filtering the eluate with a filter to produce a filtrate, evapo
vacuum pump with or without elevating the temperature . rating the solvent from the filtrate with a distiller to produce
The term " extract ” refers to a solution that has been a distillate , and purging the distillate under conditions suf
purged or dehydrated to remove residual solvent . In the ficient to remove residual solvent, thereby producing a
methods of the invention , the extract is formed by purging cannabis oil extract. In certain embodiments , the eluate
or dehydrating the distillate using any known means in the 60 obtained from the cannabis plant material further includes
art . one or more terpenes , terpenoids, or other plant components .
The terms “ winterizing ” and “ freezing ” refer to cooling In some embodiments , the method further comprises mixing
an eluate from a cannabis plant to below ambient tempera a quantity of vitamin E with the extract to produce a
tures. In some instances , winterizing is used to remove cannabis oil composition . In some embodiments, the
unwanted or non - desirable compounds from the eluate . In 65 method further includes combining the cannabis oil or
some instances, winterizing is used to store the eluate before cannabis oil/vitamin E mixture with an essential oil or a
further processing. carrier oil to produce a cannabis oil composition .
 US 10,806,707 B2
7 8
Reference is now made to FIG . 1A , which is a method 100 Sour Tsunami; Stinky Purple; Strawberry Cough ; Sunset
of preparing the cannabis oil composition according to one Sherbert; Super Lemon Haze ; Super Silver Haze ; Sunra;
embodiment. The method 100 can include freezing cannabis Sweetooth SFV ; Tahoe OG Kush ; Thin Mints ; Tangerine
plant material 103 and solvent 105 in step 102 . Dream ; Tora Bora ; Trainwreck ; Ultraviolet; Unicorn ;
In some embodiments, the cannabis plant material 103 5 Vanilla Kush ; West Point Snow ; White Erkle ; White Rhino;
can be plant material from a cannabis indica plant. In some White Russian ; White Widow ; and Wizard's Potion .
embodiment, the cannabis plant material 103 can be plant The cannabis plant material 103 can include cannabis
material from a cannabis sativa plant. In some embodiment, flowers, buds , trichomes , leaves, stems, portions therein or
the cannabis plant material 103 can be plant material from combinations thereof. In some embodiments, the cannabis
a hybrid cannabis indica and cannabis sativa plant. In these 10 plant material consists essentially of cannabis buds . The
and other embodiments, the cannabis plant material 103 can buds can be whole buds or buds that are cut or broken into
be fresh plant matter. pieces . Step 102 can include freezing the cannabis plant
In some embodiments, the cannabis plant material is a material 103 and the solvent 105 for at least about 12 hours
strain selected from the group consisting ofAC /DC , Afghan (e.g. , about 16-24 hours ). In one or more embodiments, the
Goo , Atomic Northern Lights, Blackberry Kush , Blueberry, 15 cannabis plant material and the solvent can be frozen at a
Blueberry Kush, Blueberry Muffin Top , Blueberry OG , Blue temperature between about 0 ° C. and about -20 ° C. One
Diesel , Blue Dream , Buddha Passion , Cannatonic, Choco unexpected benefit from freezing the cannabis plant material
late Kush , Fire OG , Jilly Bean , Gran Daddy Purple, Grape 103 , the solvent 105 , or a combination thereof is the pres
Blackberry Kush, Harle OG , Harle Tsu , Harlequin , Hope ervation of valuable terpenes or other volatile molecules
Springs, Infinite Euphoria, Long Valley Royal Kush, Medi- 20 when preparing the cannabis oil extract. In addition , freez
haze , Pineapple Jack, Prize Kush , Sour Diesel , Sour Kush , ing the cannabis plant material and / or solvent can decrease
and Tahoe OG . the quantity of chlorophyll in the cannabis oils ( an unwanted
In some embodiments, the cannabis plant material is a byproduct of the process ).
strain selected from the group consisting of AC/DC , Blue In general, the plant material and /or extraction solvent are
berry, Afghan Goo , Prize Kush , Medihaze, and Cannatonic . 25 held at a particular temperature for a period of time sufficient
In a more specific embodiment, the cannabis plant material to ensure that the materials reach the temperature . One of
103 can be a strain of cannabis selected from the group skill in the art will appreciate that the length of cooling or
consisting of AC /DC , Blueberry, and Cannatonic . freezing time will depend in part on factors such as the
Further strains and hybrid strains contemplated for use in targeted freezing /cooling temperature and the quantity of
the methods of the invention include, but are not limited to : 30 materials used in the method, as well as the particular
Afgoo; Afghan Kush ; Agent Orange; AK - 47 ; Amnesia Haze ; extraction solvent and cannabis strain . Accordingly, canna
Atomic Jam ; Atomic Northern Lights ; Avidekel; BC Grape bis plant material and /or extraction solvents are typically
fruit; Belladonna; Berry White ; Blackberry British Colum held for periods of time ranging from several minutes
bia ; Blackberry Kush ; Black Romulan ; Black Queen ; Blue several hours in length. For example, cannabis plant mate
berry Kush ; Blueberry OG ; Blue Dream ; Blue Cheese ; 35 rial and / or extraction solvents can be held at a reduced
Blueberry Cheese; Blue Diesel ; Blue Dream ; Blue Jay Way ; temperature for anywhere from about 10 minutes to about 72
Blue Velvet; Boost ; Bubba Kush ; Bubble Gum ; Buddha hours prior to extraction . Cannabis plant material and / or
Passion ; BW Cookies ; Cadillac Purple; Canna Sue ; Can extraction solvents can be held at a reduced temperature for
naTsu ; Casey Jones , Charlotte's Web ; Cheese ; Cheeze; a period of from about 30 minutes to about 48 hours, or from
Cherry AK ; Cherry Cola ; Cherry Pie ; Chemdawg; Chem 40 about 1 hour to about 36 hours , or from about 4 hours to
Scout ; Chocolate Kush; Chocolope ; Chiesel ; Cinderella 99 ; about 24 hours , or from about 12 hours to about 18 hours
Cotton Candy Kush ; Critical Jack ; Death Star ; Diesel Cook prior to extraction . Cannabis plant material and / or extrac
ies ; Downtown Diesel ; Double Diesel ; Dream Kush ; Durban tion solvents can be held at a reduced temperature for a
Cookies ; Durban Poison ; Dutch Treat; Dr. Tod; Elektra ; period of about 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , or 18
Exodus; Fern Dog ; Fire OG ; Frankenstein OG ; G13 ; God's 45 hours prior to extraction . In some embodiments, cannabis
Gift; Gran Daddy Purps; Granddaddy Purple; Granny plant material and ethanol are held at around -20 ° C. for
Durkel; Grape Ape; Grape Puff; Grapefruit Rom ; Gra around a minimum of 16 hr prior to extracting the cannabis
pekush ; Grape Blackberry Kush ; Girl Scout Cookies ; Green plant material with the ethanol.
Crack ; Green Goddess; Headband; Heady Kush ; Harlequin ; Typically , the materials used in the methods of the present
Hash Plant; Hindica ; Hindu Kush ; Hopesprings; Huckle- 50 invention are cooled to temperatures below ambient tem
berry; Hubba Bubba ; Infinite Euphoria; Island Sweet Skunk; perature (i.e. , below about 25 ° C. ) prior to and / or during the
Jack Herer; Jamaican Lion ; Jamaican Skunk ; Jelly Bean ; extraction step . For example, the cannabis plant material
Jilly Bean ; Kushage; LA Confidential; Larry OG ; Lavender; and / or the extraction solvent can be held at a temperature
Lemon Haze ; Lemon Kush ; Lemon Skunk ; Liberty Haze ; ranging from about –80 ° C. to about 20 ° C. The cannabis
Lion Fire; Manawell; Mango; Mango Haze ; Maplewreck ; 55 plant material and /or the extraction solvent can be held at a
Master Kush ; Maui Waui; Misty ; Mr. Nice ; Northern Lights; temperature ranging from about –80 ° C. to about -20 ° C. , or
NYC Diesel ; OG Afgani; OG Kush ; Ol ’ Betsy ; Orange from about -20 ° C. to about 0 ° C. , or from about 0 ° C. to
Crush ; Orange Kush ; Phenom Phen ; Pineapple Express; about 4 ° C. , or from about 4 ° C. to about 20 ° C. In some
Pineapple Haze ; Pineapple Jack ; Pineapple Kush ; Pineapple embodiments, the cannabis plant material and the extraction
Thai ; Platinum Cookies ; Platinum Kush ; Pomegranate 60 solvent are held at about 0 ° C. prior to the extraction step .
Kush ; Purps; Purple Diesel ; Purple Goo ; Purple Hash Plant; In some embodiments, the cannabis plant material and the
Purple Haze ; Purple Jasmine ; Purple Kush ; Purple Nice ; extraction solvent are held at about -20 ° C. prior to the
Purple Platinum ; Purple Trainwreck ; Purple Urkle ; R4 ; extraction step . In some embodiments, the cannabis plant
Rain ; Red Raspberry Kush ; Romulan ; Royal Cookies; Sage material and the extraction solvent are held at about -23 ° C.
Diesel ; Sensi Star ; Sierra ; Sierra Purple; Silver Diesel ; 65 prior to and / or during the extraction step .
Silver Dragon ; Silver Haze ; Skywalker; Skywalker OG ; The extraction step can be conducted at temperatures
Snow Cap ; Sour Boogie ; Sour Diesel ; Sour Kush ; Sour OG ; ranging from about -80 ° C. to about 30 ° C. The extraction
 US 10,806,707 B2
9 10
step can be conducted , for example , at a temperature ranging about 4 L : 1 lb to about 8 L : 1 lb. The ratio of solvent to
from about -80 ° C. to about -20 ° C. , or from about -20 ° C. cannabis plant material can also include from about 2.5 : 1 to
to about 0 ° C. , or from about 0 ° C. to about 4 ° C. , or from about 3.5 : 1 , from about 2.3 : 1 to about 3.7 : 1 , from about
about 4 ° C. to about 20 ° C. In some embodiments, the 2.2 : 1 to about 3.8 : 1 , from about 2 : 1 to about 4 : 1 , from about
extraction step is conducted a temperature below about 0 ° C . 5 1.8 : 1 to about 4.2 , or from about 1.5 : 1 to about 4.5 : 1 . In
In some embodiments, the extraction step is conducted at some embodiments, the extraction solvent to cannabis plant
about -23 ° C. The extraction can be conducted with mate material ratio in the extraction step is about 3L : 1 lb.
rials that have been frozen or chilled as described above or In one embodiment, three liters of the solvent 105 can be
with materials at ambient temperatures. poured over one pound of the cannabis plant material 103 .
The solvent 105 can be a predominantly polar solvent. In 10 In a more specific embodiment, the solvent 105 can be
one embodiment, the solvent 105 can be an alcohol such as organic ethanol.
ethanol. The solvent 105 can also be a polar solvent derived In one or more embodiments, the eluate or menstruum
from organic sources . In a more specific embodiment, the collected from this pouring step can be poured over the same
solvent 105 can be a 95 % biodynamic ethanol. In an even cannabis plant material 103 again to elute more of the
more specific embodiment, the solvent 105 can be 190 proof 15 cannabinoids 107 from the cannabis plant material 103. This
organic grain wheat spirit. In other embodiments, the solvent pouring step can be repeated until the cannabis plant mate
105 can include organic ethers, esters , and / or ketones. In rial 103 has been poured over a total of three to six times ,
some embodiments, the solvent can include USDA certified or until the coloration of the eluate or menstruum exhibits
organic corn , grape, or cane sugar, food - grade organic hues of green due to accumulation of chlorophyll or other
alcohol , and /or biodynamic ethanol. 20 undesired plant material in the eluate .
One of skill in the art will appreciate that a large number Any number of pouring steps can be used to elute the
of organic solvents can be used for this extraction. Examples cannabinoids from the cannabis plant material during the
of organic solvents that can be used include , but are not extraction step . The number of pours can range from 1 to
limited to , acetonitrile , methanol , isopropanol , 1 - butanol , about 15 or more . For example , there can be 1 , 2 , 3 , 4 , 5 , 6 ,
2 -butanol, dichloromethane, ethyl acetate , isopropyl acetate , 25 7 , 8 , 9 , 10 , 11 , 12 13 , 14 , 15 or more pouring steps . In some
isopropyl ether, methyl tert-butyl ether, diethyl ether, embodiments, multiple pouring steps are achieved by reus
acetone , butane , hexane , heptane, and combinations thereof. ing the collected eluate or menstruum of the initial pouring
In some embodiments, isopropanol is used as the extraction step . In some embodiments, multiple pouring steps are
solvent. In some embodiments, ethyl acetate is used as the achieved by using fresh extraction solvent. In some embodi
extraction solvent. In some embodiments mixtures of 30 ments the volume of extraction solvent is altered in different
organic solvents can be used to improve the extraction pouring steps . In some embodiments, the number of pouring
process. steps is terminated before the eluate turns green, which color
The method 100 can also include eluting cannabinoids can indicate an undesirable level of chlorophyll or other
107 , such as THC and CBD , from the cannabis plant undesired plant material accumulation in the eluate .
material 103 with the solvent 105 to produce an eluate 109 35 At this point, the eluate or menstruum produced by the
in step 104. In one embodiment, step 104 can include eluting repeated pours can be filtered to yield the first eluate 111 .
the cannabinoids 107 from the cannabis plant material 107 The eluate or menstruum produced by the repeated pours
frozen in step 102 with the solvent 105 also frozen from step can be filtered by pouring through a mesh filter. In one
102 representing an eluent. The cannabis plant material 103 embodiment, the mesh filter can be a metallic filter. In some
can also be referred to as a marc . The eluate 109 can also be 40 embodiments , the mesh filter can be a membrane filter. In
referred to as a menstruum . some embodiments, the mesh filter can be a cloth or muslin
As will be discussed in more detail below, step 104 can fabric filter.
yield a first eluate 111 and a second eluate 113. For ease of The first eluate 111 can be collected in a glass or other
reference , both the first eluate 111 and the second eluate 113 container having a lid or other closing mechanism . In one
can be referred to as the eluate 109 . 45 embodiment, the glass container can be a glass jar having a
In one embodiment, step 104 can include placing the jar lid . In a more specific embodiment, the glass container
cannabis plant material 103 in a strainer or perforated filter can be a gallon- sized glass jar. In some embodiments, the
funnel over a collection receptacle . In a more specific glass container can be a 2-5 L Pyrex media bottle . The glass
embodiment, the strainer can be a colander such as a ROSLE container comprising the first eluate 111 can be closed by the
colander and the collection receptacle can be a bucket or 50 lid or other closing mechanism and stored in a freezer. In one
other type of open container. In other embodiments, the embodiment, the first eluate 111 collected in the glass
strainer can be a sieve or straining basket. In these embodi container can be stored for about 24 to about 48 hours at a
ments, the cannabis plant material 103 frozen from step 102 temperature between about 0 ° C. and about -20 ° C. After
can be placed in the strainer over the collection receptacle . this freezing step, the first eluate 111 can undergo further
Step 104 can include pouring the solvent 105 representing 55 filtration in step 108 below . In some embodiments, the first
the eluent over the cannabis plant material 103 placed in the eluate is further subjected to solarization as described below .
strainer and collecting the eluate or menstruum from this Step 104 can also include using the leftover cannabis
pouring step in the collection receptacle. plant material 103 from the pouring steps above to produce
Any amount of solvent suitable for extracting cannabi the second eluate 113. Fresh portions of the solvent can be
noids and other desired compounds can be used in the 60 poured over cannabis plant material 103 in the strainer to
methods of the invention . For example, the ratio of extrac produce the second eluate . Alternatively, the cannabis plant
tion solvent ( L ) to cannabis plant material ( lb ) in the material 103 can be removed from the strainer and placed
extraction step can range from about 0.1 L : 1 lb to about 10 into an open container. In one embodiment, the open con
L : 1 lb or more . The extraction solvent to cannabis plant tainer can be a bucket such as a polymer -based bucket . As
material ratio can be from about 0.1 L : 1 lb to about 1 L : 1 lb , 65 a more specific embodiment, the open container can be a five
from about 1 L : 1 lb to about 2 L : 1 lb , from about 1 L : 1 lb gallon plastic bucket . In this embodiment, fresh instances of
to about 2 L : 1 lb , from about 2 L : 1 lb to about 4 L : 1 lb , from the solvent 105 ( e.g. , unused solvent 105 from step 102 ) can
 US 10,806,707 B2
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be poured into the open container until the solvent 105 difference in absorption between wavelengths around 650
completely covers the cannabis plant material 103. The nm ( red ) and around 940 nm ( infrared ). The measurement of
second eluate can be subjected to solarization as described the ODD between these two wavelengths can be used to
below. determine the chlorophyll content in the cannabis extract.
Step 104 can further include soaking the cannabis plant 5 One of skill in the art will recognize that there are other
material 103 in the solvent 105 , at or below room tempera techniques available to determine the amount of chlorophyll
ture, for about 1 to about 2 hours in the open container. In remaining in extracts .
some embodiments, the plant material is left to soak without The method 100 can include solarizing the second eluate
agitation . In one embodiment, the cannabis plant material 113 in step 106. For illustrative purposes, solarizing the
103 can also be macerated while soaking in the solvent 105. 10 eluate 109 will be described with respect to the second eluate
In this embodiment, the cannabis plant material 103 can be 113 , although it should be understood that any type of eluate
macerated by agitating the cannabis plant material 103 or menstruum produced from the cannabis plant material
through mechanical or manual force such as by stirring the 103 can be solarized to remove the effects of chlorophyll
solvent 105 in the open container. The plant material can from the eluate 109 .
also be broken apart or ground into finer -sized particles . 15 Step 106 can involve exposing the second eluate 113 to
The extraction solvent can be soaked with the plant direct sunlight in order to solarize the second eluate 113. In
material before straining or the extraction solvent can be one embodiment, the glass container comprising the second
kept separate before straining. In instances where cannabis eluate 113 can be placed in direct sunlight for at least two
plant is soaked /macerated with extraction solvent, incuba hours . In other embodiments, a plasma light emitter can be
tion time can range from less than about 1 minute to more 20 used to direct light at the second eluate 113 at a light
than about 10 hours. For example, incubation time ranges intensity between about 500 to about 2000 photosynthetic
from less than 1 minute to about 10 minutes, from about 10 photon flux (PPF or umol m- as - l ) for approximately 8 to 10
minutes to about 30 minutes, from about 30 minutes to about hours .
2 hours, from about 2 hours to about 4 hours, from about 4 Solarization can be accomplished using any source of
hours to about 7 hours, or from about 7 hours to about 10 or 25 light suitable for degrading chlorophyll. The light source can
more hours. In some embodiments the extract and the plant be , for example, the sun . Another source of light used can be
material are soaked /macerated for about 2 minutes. In some non - natural light sources . Non - natural light sources can
embodiments, the extract and the plant material are soaked / include those that emit a full light spectrum in an attempt to
macerated for about 2 hours. In some embodiments , the mimic natural light, or those that only provide specific
extract and the plant material are soaked /macerated for 30 wavelengths. Non - natural light sources can also include
about 6 hours . those that vary spectral outputs and temperatures as time
After soaking the cannabis plant material 103 in the passes , or those that keep a constant spectral output and
solvent 105 , the entire contents of the open container can be temperature. In some embodiments the light source is sun
poured through a strainer, such as a ROSLE colander, and light. In some embodiments the light source is a plasma light
then filtered to yield the second eluate 113. The contents of 35 (e.g. , a Gavita Pro 300 light emitting plasma lamp equipped
the open container can be filtered using a mesh filter. In one with LUXIM STA 41.02 LiFi light source ). The plasma light
embodiment, the mesh filter can be a metallic filter. In some can be a full -spectrum plasma light including UVB light.
embodiments , the mesh filter can be a membrane filter or a The solarization step can be conducted at any temperature
fabric (e.g. , muslin ) filter. suitable for degrading, or otherwise reducing, the chloro
The second eluate 113 can be collected in a glass or other 40 phyll in the extract. Typically, solarization will be conducted
container ( e.g. , a container made of high- or low - density at a temperature ranging from about -80 ° C. to about 30 ° C.
polyethylene ) having a lid or other closing mechanism . In The solarization step can be conducted , for example, at a
one embodiment, the glass container can be a glass jar temperature ranging from about -80 ° C. to about -20 ° C. , or
having ajar lid . The second eluate 113 can be subjected to from about -20 ° C. to about 0 ° C. , or from about 0 ° C. to
solarization in step 106 prior to further filtration in step 108 45 about 4 ° C. , or from about 4 ° C. to about 20 ° C. In some
below. embodiments, the solarization step is conducted a tempera
Solarization is a process that includes exposing the can ture below about 0 ° C. In some embodiments , solarization
nabis extract to a light source to degrade any chlorophyll step is conducted at about -23 ° C.
that has collected with the cannabinoids. The solarization In all such embodiments, the solarization of the second
process can be carried out for any amount of time desired . 50 eluate 113 can cease when the color of the second eluate 113
Typically, the incubation time can range from fewer than no longer exhibits a green hue or turns from a green color to
about 5 minutes to more than about 12 hours . The solariza a yellowish - brown color. It has been discovered that the
tion time can depend on factors including, but not limited to , solarization step allows oil producers to elute more of the
the strength of the light source used . The solarization time cannabinoids 107 from the same batch of the cannabis plant
can be from about 5 minutes to about 30 minutes, or from 55 material 103 through the two - step process described above .
about 30 minutes to about 2 hours , or from about 2 hours to More specifically, the solarization step allows oil producers
about 5 hours, or from about 5 hours to about 12 hours or to make the cannabis oil extract from the second eluate 113
more . The solarization time can also depend on the desired without leaving undesirable amounts of chlorophyll into the
finished product. In some embodiments, solarization is car final product. In one embodiment, the level of cannabinoids
ried out for about 2 hours. In some embodiments, solariza- 60 107 of the first eluate 111 and the second eluate 113 are
tion is carried out for about 10 hours . In some embodiments, assayed using high -performance liquid chromatography
solarization is carried out until the extract changes from a (HPLC ) and ultraviolet ( UV ) detectors . In this embodiment,
nettle green color to a yellow -brown color. In some embodi the second eluate 113 contains about 10 % less cannabinoids
ments, solarization is carried out until the optical density 107 than the first eluate 111 .
difference ( ODD ) of the solution reaches a value indicating 65 Generally, after the solarization step , the eluate is cooled
acceptable chlorophyll levels in the cannabis extract, as to temperatures below ambient temperature ( i.e. , below
measured on a UV - vis spectrophotometer measuring the about 25 ° C. ) . For example, the eluate can be held at a
 US 10,806,707 B2
13 14
temperature ranging from about –80 ° C. to about 20 ° C. The evaporation time required will vary. In some embodiments ,
eluate can be held at a temperatures ranging from about -80 ° the filtrate is evaporated for time intervals ranging from
C. to about -20 ° C. , or from about -20 ° C. to about 0 ° C. , about 30 minutes to about 2 hours, from about 2 hours to
or from about 0 ° C. to about 4 ° C. , or from about 4 ° C. to about 4 hours , from about 4 hours to about 6 hours, from
about 20 ° C. In some embodiments, the eluate is held at 5 about 6 hours to about 8 hours, or from about 8 hours to
about 0 ° C. In some embodiments , the eluate is held at about about 10 hours . In some embodiments, the filtrate is distilled
-20 ° C. or evaporated for about 2 hours. In some embodiments , the
One of skill in the art will appreciate that the length of filtrate is distilled or evaporated for about 8 hours. In some
cooling time will depend in part on factors such as the embodiments, step 110 can include distilling the filtrate 115
targeted freezing / cooling temperature and the quantity of 10 for at least 4 hours .
materials used in the methods. Accordingly , the eluate is After evaporating the solvent from the filtrate, the distil
typically held for periods of time ranging from several late can be optionally heated above room temperature under
minutes to several hours in length . For example, eluate can controlled conditions for an additional period of time . In
be held at reduced temperatures for about 5 minutes to about some embodiments, the distillate is heated at a controlled
3 days or more . In some embodiments, the eluate can be held 15 temperature for a period of time sufficient to convert acidic
at reduced temperatures from about 5 minutes to about 1 cannabinoids to neutral cannabinoids via decarboxylation .
hour, from about 1 hour to about 5 hours , from about 5 hours The distillate, after evaporation and optional heating, is
to about 24 hours, from about 24 hours to about 48 hours , transferred to an appropriate heating flask . A condenser with
from about 48 hours to about 96 hours or more . In some recirculating chilling fluid is attached on top of the appro
embodiments, the eluate is held at reduced temperatures for 20 priate heating flask to condense oil vapors during the heating
about 24 hours . In some embodiments, the eluate is held at process.
reduced temperatures for about 48 hours. Accordingly, some embodiments of the invention provide
The second eluate 113 can be stored for about 24 to about a method for preparing a cannabis oil extract as described
48 hours at a temperature between about 0 ° C. and about above , wherein the method further includes heating the
-20 ° C. After this freezing step , the second eluate 113 can 25 distillate under conditions sufficient to form a decarboxy
undergo further filtration in step 108 below . lated distillate .
The method 100 can further include filtering the eluate After distillation and optional heating, the distillate can be
109 , including the first eluate 111 , the second eluate 113 , or optionally filtered through a solid -phase filter medium .
a combination thereof, with a filter to produce a filtrate 115 Examples of suitable solid - phase filter media include, but
in step 108. In one embodiment, step 108 includes filtering 30 are not limited to , silica gel , activated charcoal, activated
the eluate 109 using vacuum filtration . In a more specific carbon , diatomaceous earth ( Celite ) , and ion -exchange res
embodiment, step 108 can include pouring the eluate 109 ins . In some embodiments, the solid -phase filter medium is
through a Büchner funnel coupled a vacuum or side - arm silica gel . The distillate can be homogenized or otherwise
flask . In these and other embodiments , the Büchner funnel combined with a suitable solvent prior to the optional
can represent the filter. 35 filtration step . The homogenized distillate can then be added
In this embodiment, one or more pieces of filter paper can to a portion of silica gel that has been conditioned ( pre - run )
be placed in the Büchner funnel and a vacuum pump can be in a suitable filter apparatus with the same solvent as added
used to provide vacuum suction . In one embodiment, the to the distillate. Once the homogenized distillate is fully
filter paper can have a pore size of between 12-25 microm absorbed on the silica , additional solvent can be added on
eters ( um ). As a more specific embodiment, the filter paper 40 top of the settled silica . During the silica gel filtration step ,
can be a WhatmanTM ashless Grade 589 filter paper. In this the homogenized distillate and added solvent can be pulled
embodiment, two pieces of the filter paper can be placed in through the filter apparatus using a light vacuum or pushed
the Büchner funnel to filter the eluate 109 . through the filter apparatus using positive pressure applied
Step 108 can also include freezing the Büchner funnel from above. Alternatively, the homogenized distillate can
prior to pouring the eluate 109 into the funnel. In addition, 45 proceed through the apparatus via gravity filtration . The
step 108 can include wetting the filter paper with the solvent filtrate can be collected in an appropriate flask prior to
105 prior to pouring the eluate 109 into the Büchner funnel. removal of solvent via evaporation, as described above .
The filtrate 115 can be collected from the vacuum or The solvent used in homogenizing the distillate can be
side - arm flask and undergo evaporation in step 110 . any of the solvents discussed above, including ethanol, ethyl
The method 100 can further include evaporating the 50 acetate , or heptane. The ratio of solvent added can range
solvent 105 from the filtrate 115 to produce a distillate 117 from about 1 mL solvent to about 1 g of distillate ( 1 : 1 ) to
in step 110. In some embodiments, the filtrate 115 can be about 4 : 1 (mL solvent to g of distillate ) . For example , the
distilled using a distiller. In some embodiments, the filtrate ratio of solvent to distillate can be from about 1 : 1 to about
can be distilled using an evaporator. In some embodiments , 2 : 1 , from about 2 : 1 to about 3 : 1 , or from about 3 : 1 to about
the evaporator can be a rotary evaporator. In some embodi- 55 4 : 1 . In some embodiments, the ratio of solvent to distillate
ments, the distiller can include an essential oil distiller. As a is about 2 : 1 . In some embodiments, the ratio of solvent to
more specific embodiment, the distiller can be a Mega distillate is about 3 : 1 .
homeTM DA4B distiller. The filtrate 115 can be distilled by Silica gel can be added to the homogenized distillate in
separating the solvent 105 from the remainder of the filtrate any amount suitable for removing unwanted components via
115 through a selective evaporation and condensation pro- 60 filtration . Silica gel can be added , for example, in an amount
cedure . ranging from about 1 g of added silica for every 1 g of
The filtrate can be distilled or evaporated for any length homogenized distillate ( 1 : 1 ) to about 3 g of added silica for
of time , depending on the desired concentration of distillate . every 1 g of homogenized distillate (3 : 1 ) . The amount of
For example, the filtrate can be distilled or evaporated for added silica added to homogenized distillate can range from
anytime ranging from about 30 minutes to about 10 hours or 65 about 1 : 1 to about 2 : 1 , or from about 2 : 1 to about 3 : 1 . In
more . An ordinary skilled artisan will recognize that depend some embodiments the ratio of added silica to homogenized
ing on the exact method and machinery used, the exact distillate is about 2 : 1 . Additional silica gel is used as the pad
 US 10,806,707 B2
15 16
or be in the filtration step . Typically, the additional silica gel from about 1 mbar to about 10 mbar, or from about 10 mbar
is used in amounts ranging from about 3 silica for every to about 20 mbar, or from about 20 mbar to about 50 mbar,
1 g of homogenized distillate ( 3 : 1 ) to about 9 : 1 . For or from about 50 mbar to about 100 mbar, or from about 100
example, the ratio of additional silica to homogenized dis 5
mbar to about 200 mbar, or from about 200 mbar to about
tillate can range from about 3 : 1 to about 4 : 1 , from about 4 : 1 500 mbar. In some embodiments the solvent purging pres
to about 5 : 1 , from about 5 : 1 to about 6 : 1 , from about 6 : 1 to sure is about 10 mbar. In some embodiments , the solvent
about 7 : 1 , from about 7 : 1 to about 8 : 1 , or form about 8 : 1 to purging pressure is about 50 mbar. In some embodiments,
about 9 : 1 . In some embodiments , the ratio of additional the solvent purging pressure is about 100 mbar. In some
silica to distillate is about 6 : 1 . In some embodiments, the 10 embodiments, the solvent purging pressure is about 250
ratio of additional silica to distillate is about 4 : 1 . In some
embodiments the additional silica is loaded into the funnel mbar. Reduced pressures can be obtained using any suitable
apparatus including, for example, an Across International
alone . In some embodiments, the additional silica gel is Vacuum Oven (Model VO - 16050 ) or a Buchi Multivapor
loaded into the funnel with the same solvent used to homog 15 apparatus equipped with a vacuum pump. In some embodi
enize the distillate .
Accordingly, some embodiments of the invention provide ments in a
, the distillate is purged while being stirred and heated
heavy -walled flask under reduced pressure .
a method for preparing a cannabis oil extract as described During the purge /dehydration step, the distillate may be
above , wherein the method further includes heating the
optionally heated to increase the efficiency of the solvent
distillate under conditions sufficient to form a decarboxy- 20 purge . The temperature used for purging/dehydration can be
lated distillate and filtering the decarboxylated distillate to any temperature
form a decarboxylated filtrate . at or above ambient conditions. For
The method 100 can further include dehydrating or purg range from about 20during
example , heating
° C. to
the purge /dehydration step can
about 200 ° C. or more . In some
ing the distillate 117 ( after optional filtration and heating) to
instances the purge /dehydration temperature can range from
further remove any further traces of the solvent 105. In 25 about
doing so , the dehydration produces an extract 119 in step about above 20 ° C. to about 35 ° C. , or from about 35 ° C. to
50 ° C. , or from about 50 ° C. to about 65 ° C. , or from
112. Dehydration can be achieved using any known means about 65 ° C. to about 90 ° C. , or from about 90 ° C. to about
in the art including the use of a food dehydrator, evaporator, 130 ° C. , or from about 130 ° C. to about 170 ° C. , or from
or vacuum pump. In some embodiments the distillate is 30 about 170 ° C. to about 200 ° C. or more . In some embodi
placed in an open container. In some embodiments the ments, the purge /dehydration temperature is about 35 ° C. In
distillate is place in a sealed container where air pressure can some embodiments , the purge / dehydration temperature is
be lowered . about 50 ° C. In some embodiments, the purge/dehydration
In general, purging /dehydration is conducted under con temperature is about 55 ° C. In some embodiments, the
ditions sufficient to remove residual solvent from the can- 35 purge /dehydration temperature is about 70 ° C. In some
nabis oil extract . “ Residual solvent ” refers to any solvent embodiments , the purge /dehydration temperature is about
( e.g. , ethanol) used during the extraction process that 90 ° C. In some embodiments, the purge /dehydration tem
remains in the extract after the elution , solarization , filtra perature is about 110 ° C.
tion, and evaporation steps. The removal of residual solvent 40 A person of skill in the art will recognize that the time of
can be monitored , for example , by conducting the purge/ dehydration required to remove the remaining solvent will
dehydration step until the weight of the extract stops depend on the pressure and temperature of the purge!
dehydration step as well as the solvent that is being
decreasing ( indicating that all volatile solvent has been removed
removed ). In some embodiments, removing residual solvent from anywhere . Typically, the time of the purge step will range
refers to removing at least 90 % of the ethanol used in the 45 example, the timebetween about 1 hour and about 5 days . For
extraction process from the cannabis oil extract. In some about 1 day, from about 1 daycantorange
of purging from about 1 hour to
embodiments, removing residual solvent refers to removing 2 days to 3 days, or from about 3 days to2 days about , from about
at least 95 % of the ethanol used in the extraction process days. In some embodiments, the time of purging is5 aboutabout or more
from the cannabis oil extract. In some embodiments, remov 50 hours . In some embodiments , the time of purging is about182
ing residual solvent refers to removing at least 99 % of the days. In some embodiments, the time ofpurging can is about
ethanol used in the extraction process from the cannabis oil 3 days. In some embodiments, the time of purging can is
extract. about 4 days . In some embodiments, the time of purging is
In some embodiments , the dehydrator can be a food about 5 days.
dehydrator. In a more specific embodiment, the dehydrator 55 After obtaining the extract 119 , the composition of the
can be an ExcaliburTM food dehydrator. Step 112 can involve extract can be determined by a variety of the methods. For
placing the petri dishes comprising the distillate 117 into the example , a portion of the extract can be analyzed by
dehydrator. In one embodiment, the dehydrator can be set at methods including, but not limited to , liquid chromatogra
about 55 ° C. The distillate 117 can be dehydrated for at phy /mass spectrometry (LC - MS ) , gas chromatography /mass
anywhere between about 1 and about 72 hours, or longer, to 60 nance
spectrometry (GC-MS (' H),-NMR
and proton nuclear, themagnetic reso
yield the extract 119. In other embodiments, the distillate of the spectroscopy ). In addition composition
extract 119 can be organoleptically tested to ensure
117 can be dehydrated for up to about 120 hours.
In some embodiments, dehydration of residual solvent nation thereofin. taste , smell , texture, coloration, or a combi
consistency
can be achieved with vacuum pumps providing reduced 65 As an example, Table 1 below shows the amount and
pressure levels ranging from about 1 mbar to about 500 percent yields of extract 119 from varying amounts of
mbar. In some instances, solvent purging is carried about cannabis plant material 103 :
 US 10,806,707 B2
17 18
TABLE 1 vitamin E is added to the cannabis oil in an amount sufficient
to provide a viscosity of about 2500 CP.
Yield Results from Cannabis Plant Material to Extract In one preferred embodiment, the quantity of vitamin E
Cannabis Plant Amount of mixed with the extract 119 is about 30 percent weight by
Material Solvent, Extract Yield Extract Yield 5 weight (30 % w / w ) based on a total weight of the cannabis
Cannabis Plant Amount Ethanol Amount Percentage oil composition. In other embodiments, the quantity of
Material Strain ( grams) (L ) ( grams) (% ) vitamin E mixed with the extract 119 can be between about
AC / DC 680.39 7.00 42.0 6.17 30% w / w and about 50 % w/w based on the total weight of
Blueberry
Cannatonic
1315.41 13.0 79.8
41.4
6.07
6.08
the cannabis oil composition.
680.38 10.5 10 In these and other embodiments , the vitamin E 121 can be ,
but is not limited to , vitamin E derived from organic sources .
The method 100 can further include mixing a quantity of For example, the vitamin E 121 can be vitamin E derived
vitamin E 121 with the extract 119 to yield a cannabis oil from organic sunflowers. As a more specific embodiment,
composition . the vitamin E 121 can be DevaTM non - genetically modified
15
The amount of vitamin E added to the extract can depend can(non -GMO ) vitamin E from sunflowers. The vitamin E 121
on factors including the strain of cannabis plant used and the include tocopherols and tocotrienols. More specifically,
desired viscosity of the extract. The amount of vitamin E vitamin E 121 can include a - tocopherol.
added to the extract will typically range from about 0 % Step 114 can include placing a suitable vessel , such as a
beaker or petri dish , comprising the extract 119 on a hotplate
( w / w ) to about 95 % . The amount of vitamin E added to the 20 set at about 60-95° C. Step 114 can further include mixing
extract can range , for example, from about 0.5 % to about the vitamin E 121 with the extract 119 by gently stirring the
5 % , or from about 5 % to about 10 % , or from about 10% to vitamin E 121 into the extract 119 warmed on the hotplate
about 15 % , or from about 15 % to about 20% , or from about until the cannabis oil composition is homogenized. In
20 % to about 25 % , or from about 25 % to about 30 % , or from another embodiment, mixing the vitamin E 121 with the
about 30 % to about 35 % , or from about 35 % to about 40 % , 25 extract 119 can involve injecting a quantity of the vitamin E
or from about 40% to about 50 % , or from about 50 % to 121 into the extract 119. In some embodiments, mixing the
about 60 % , or from about 60 % to about 70 % or more . The vitamin E with the extract can involve adding a quantity of
amount of vitamin E added can range from about 54 % to the vitamin E into extract that is being mechanically or
about 56 % , or from about 52 % to about 58 % , or from about manually stirred and heated in a flask .
49 % to about 61 % , or from about 47 % to about 63 % , or from 30
about 46 % to about 64 % , or from about 44 % to about 66 % . III . Cannabis Oils
The amount of vitamin E added can range from about 12 %
to about 48 % , or from about 14 % to about 46 % , or from bisInoilrelated aspects , the present invention provides canna
extracts and compositions prepared by the methods
about 16 % to about 44 % , or from about 18 % to about 42 % , 35 described herein .
or from about 20% to about 40 % , or from about 22 % to A. Cannabinoids
about 38 % , or from about 24 % to about 36 % , or from about Cannabis oils of the invention can contain neutral can
26 % to about 34 % , or from about 28 % to about 32 % . In nabinoids
some embodiments , the amount of vitamin E added to the Examples, ofacidic cannabinoids, and combinations thereof.
neutral cannabinoids include , but are not lim
extract is about 15 % or more . In some embodiments, the 40 ited to : cannabigerol ( CBG) and related compounds ( e.g. ,
amount of vitamin E added is about 28 % or more . In some cannabigerol monomethyl ether, cannabigerovarin ); can
embodiments, the amount of vitamin E added is about 30 % nabichromene (CBC ) and related compounds (e.g. , ( + )
or more . In some embodiments, the amount of vitamin E cannabichromene , ( + ) - cannabichromevarin ); ( - ) - canna
added is about 44 % or more . In some embodiments, the bidiol (CBD ) and related compounds (e.g. , cannabidiol
amount of vitamin E added is about 55 % or more . 45 momomethyl ether, cannabidiol-C4, ( - )-cannabidivarin ,
In certain embodiments, vitamin E is added to the can cannabidiorcol ); cannabinodiol (CBND ) and related com
nabis oil in an amount sufficient to provide a desired pounds ( e.g. , cannabinodivarin ); Aº -tetrahydrocannabinol
viscosity level . For example, vitamin E can be added to the (THC ) and related compounds ( e.g. , Aº- tetrahydrocannabi
cannabis oil in an amount sufficient to provide a viscosity nol -CA A® -tetrahydrocannabivarin , Aº -tetrahydro - can
ranging from about 6000 CP to about 200 CP. Vitamin E can 50 nabiorcol, (-)- 48- trans-(6aR ,10aR )-48- tetrahydrocannabi
be added to the cannabis oil in an amount sufficient to nol , ( - ) - (6aS,10aR )-19 - tetrahydro -cannabinol ); cannabinol
provide a viscosity ranging from about 6000 CP to about (CBN) and related compounds ( e.g. , cannabinol-C4, can
5000 CP, or from about 5000 cP to about 4000 CP, or from nabivarin , cannabinol -C2, cannabiorcol, cannabinol methyl
about 4000 CP to about 3000 CP, or from about 3000 CP to ether ); ( 1 )-cannabitriol (CBT ) and related compounds ( e.g. ,
about 2000 cP, or from about 2000 CP to about 1000 cP, or 55 ( -) -( 9R ,10R ) -trans-10-0 - ethyl -cannabitriol, ( + )-(9R ,10R /
from about 1000 cP to about 200 CP. In certain instances, 98,10S )-cannabitriol-C3); cannabielsoin (CBE ) and related
vitamin E is added to the cannabis oil in an amount sufficient compounds (e.g. , ( 5aS ,68,9R ,9aR ) -cannabielsoin, ( 5aS , 6S ,
to provide a viscosity of less than about 3500 CP . In certain 9R ,9aR ) Cz - cannabielsoin , cannabiglendol -C3, dehydro
other instances, vitamin E is added to the cannabis oil in an cannabifuran, cannabifuran ); isocannabinoids (e.g. , (- )-A ?
amount sufficient to provide a viscosity ranging from about 60 trans-( 1R , 3R , 6R )-isotetrahydrocannabinol, (+)-AP-1,2-cis
1050 CP to about 950 CP, or from about 1100 CP to about 900 ( 1R ,3R ,6S ) -isotetrahydrocannabivarin , ( + ) - A7-1,2 - cis- ( 1S ,
cP, or from about 1150 CP to about 850 CP, or from about 3S ,6R ) -isotetrahydro -cannabivarin , ( - ) - A ’ - trans- (1R ,3R ,
1200 CP to about 800 CP, or from about 1250 CP to about 750 6R )-isotetrahydrocannabivarin ); cannabicyclol ( CBL ) and
cP, or from about 1300 cP to about 700 CP, or from about related compounds (e.g. , ( + ) - ( 1 aS ,3aR ,8bR ,8cR )-cannabi
1350 CP to about 650 CP . In some embodiments, vitamin E 65 cyclol CBL- C52 ( + ) -( las, 3aR ,SR ,ScR ) -cannabicyclo
is added to the cannabis oil in an amount sufficient to varin ); cannabicitran (CBT ) and related compounds; and
provide a viscosity of about 1000 cP . In some embodiments , cannabichromanone ( CBCN) and related compounds ( e.g. ,
 US 10,806,707 B2
19 20
cannabichromanone -C3, cannabicoumaronone ). The struc -continued
tures of various neutral cannabinoids are set forth below.

5 OH
OH
H CunulH

HO
HO 10
( -) -cannabidiol
[CBD]
cannabigerol
[ CBG] 15
OH OH
H CH

20

cannabidiol monomethyl ether

[ CBDM ]
cannabigerol monomethyl ether
[ CBGM ] 25
OH OH
H C 111H

30
HO HO

cannabidiol- C4
[CBD - C4 ]
cannabigerovarin
35
[ CBGV]
OH
HI 4.11?
40

HO
( - ) - cannabidivarin
[ CBDV]
45

OH
HO
H enllH
( + )- cannabichromene 50
[ CBC ]
HO
cannabidiorcol
55 [ CBD - D ]

OH
60

HO HO
( + ) - cannabichromevarin 65 cannabinodiol
[ CBCV] [ CBND ]
 US 10,806,707 B2
21 22
-continued -continued
OH
OH 5 H

HO 10
cannabinodivarin ( -) -48 -trans- ( 6aR , 10aR )-48-48
tetrahydrocannabinol
[CBND -C3] [ 48- THC ]
15

OH OH

20
sult
H

19- tetrahydrocannabinol 25 ( - ) - ( 6as , 10aR )-A9

[ THC ] tetrahydrocannabinol
[ cis - A9 - THC ]

30

H
.11H
OH

19 - tetrahydrocannabinol- C4
[ THC - C4 ]
35

40
go OH

cannabinol
(CBN )

OH 45 OH
IH
H?

50

A9-tetrahydrocannabivarin cannabinol- C4
[ THCV] [CBN- C4 ]
55

OH OH
IH
60
H

Aº -tetrahydrocannabiorcol 65 cannabivarin
[THC - C1 ] [CBN- C3 ]
 US 10,806,707 B2
23 24
-continued -continued
HO,
H?
OH
5

10 HO
cannabinol - C2 (5aS , 6S , OR, 9aR )-C3- cannabielsoin
[CBN- C2 ] [ CBE - C3 ]

OH 15

HO
20
HO
cannabiorcol cannabiglendo - C3
[ CBN - C ] [ OH - iso - HHCV - C3 ]
25

30
HO
cannabinol methyl ether dehydrocannabifuran
[ CBNM ]
OH
[ DCBF ]
35
IOH OH

40

HO
( - ) - ( 9R , 10R )-trans -cannabitriol
[ (-) -trans -CBT] cannabifuran
45 [ CBF ]
OH
OH

H
50

HO
( - ) - ( 98 , 10S )-trans -cannabitriol ( -) - A ? -trans- (1R, 3R, 6R )
[ (- ) - trans - CBT] 55 isotetrahydrocannabinol
HO
H

60 Hi
H
HO HO
( 5aS , 6S , 9R , 9aR ) -cannabielsoin 65 (+ ) - 47-1,2 - cis- ( 1R , 3R , 6S)
[ CBE ] isotetrahydrocannabivarin
 US 10,806,707 B2
25 26
-continued -continued
OH

5
H
H

HO
cannabichromanone - C3
[ CBCN - C3 ]
10
( +) -47-1,2 - cis- ( 1S , 3S , 6R )
isotetrahydrocannabivarin

15

H
20 cannabicoumaronone
[CBCON]
HO

(-) -47-1,2 -trans-(1R, 3R , 6R ) Examples of acidic cannabinoids include, but are not
isotetrahydrocannabivarin 25
limited to : cannabigerolic acid A ; cannabigerolic acid A
monomethyl ether, cannabigerovarinic acid A ; (+ )- cannabi
chromenic acid A ; ( + ) -cannabichromevarinic acid A ; can
nabidiolic acid ; cannabidivarinic acid ; Aº- tetrahydrocannab
inolic acid A ; 19 -tetrahydrocannabinolic acid B;
30 19- tetrahydrocannabinolic acid-C4 A ; 19- tetrahydrocannab
inolic acid -C4 B ; A -tetrahydro -cannabivarinic acid A;
Aº- tetrahydrocannabiorcolic acid A ; Aº- tetrahydrocan
HO nabiorcolic acid B ; ( - ) - A - trans - 6aR , 10aR )-tetrahydrocan
( + ) - ( laS , 3aR, SR , SR ) nabinolic acid A ; cannabinolic acid A ; (5aS ,6S , 9R , 9aR )
cannabicyclol 35 cannabielsoic acid A ; ( 5aS ,6S ,9R , 9aR )-cannabielsoic acid
[ CBL ] B ; (5aS ,6S , 9R ,9aR ) Cz- cannabielsoic acid B ; and ( + )
( 1aS ,3aR , 8bR , 8cR ) -cannabicyclolic acid A. The structures
of various acidic cannabinoids are set forth below.
40

OH
HO
45
(+ ) - ( 1aS , 3aR, 8bR, 8cR ) ??
cannabicyclovarin
[ CBLV]
HO

50

cannabigerolic acid A
[ ( E )-CBGA - C5 A ]
55

cannabicitran OH
[ CBT ]
OH OH
60

cannabichromanone 65 cannabigerolic acid A monomethyl ether

[ CBCN) [ ( E )-CBGAM - C5 A]
 US 10,806,707 B2
27 28
-continued -continued
OH

OH OH
5

HO H

10
cannabigerovarinic acid A
[ ( E )-CBGVA - C3 A ] OH
A9 -tetrahydrocannabinolic acid B
[ A - THCA B]
15

OH 0
20

??
H?

25
A9- tetrahydrocannabinolic acid- C4 A
HO [A9-THCA- C4 A]
OH
30
( + )-cannabichromenic acid A
[ CBCA- C5 A]
OH
H
35
H

OH
HI 4. ?
OH 40
OH
19 -tetrahydrocannabinolic acid- C4 B
HO [ 49-THCA-C4 B ]
cannabidiolic acid
[ CBDA] 45
OH
H?

OH ??
H
H 11H
50
OH
19- tetrahydrocannabivarinic acid A
[ A9 - THCVA A]
cannabidivarinic acid
55
[CBDVA ]
OH
1111H
OH
H
60
OH
H

??
19- tetrahydrocannabinolic acid A 65 19- tetrahydrocannabivarinic acid B
[ A - THCA A] [A9- THCVA B ]
 US 10,806,707 B2
29 30
-continued -continued
Online
OH
JIH 5

OH
H
H

HO
10
19- tetrahydrocannabiorcolic acid A
[ A - THCOA A] OH
(5aS , 6S , OR, 9aR ) -cannabielsoic acid B
[ CBEA B ]
15 HO
OH
vel
?H 20
H

HO
OH
25 OH
A9 -tetrahydrocannabiorcolic acid B
[A® - THCOA B ] ( 5aS , 6S , OR , 9aR )-C3- cannabielsoic acid B
[ CBEA- C3 B ]

30

OH

OH
H 35
HO

OH
( -) -48- trans- (6aR , 10aR )-48
tetrahydrocannabinolic acid A (+ ) - ( laS , 3aR, SR , 8cR ) -cannabicyclolic
[48- THCA A ] 40 [ CBLA A]

In general, neutral cannabinoids ( such as THC , CBD ,

CBG , CBN , and other neutral cannabinoids) are present in
45 the oils of the present invention ( e.g. , cannabis oil extracts
OH or compositions comprising same ) in amounts ranging from
about 0.001 % ( w / w ) to about 99 % ( w / w ). In certain embodi
?? ments, a neutral cannabinoid ( such as THC , CBD , CBG ,
CBN , or another neutral cannabinoid ) will be present in an
50 amount ranging from about 1 % ( w / w ) to about 99 % ( w / w ).
A neutral cannabinoid ( such as THC , CBD , CBG , CBN , or
cannabinolic acid A another neutral cannabinoid ) can be present, for example, in
[CBNA A] an amount ranging from about 0.01 % ( w / w ) to about 0.05 %
( w / w ), or from about 0.05 % ( w / w ) to about 0.1 % ( w / w ), or
55 from about 0.1 % ( w / w ) to about 0.2 % ( w / w ), or from about
HO 0.2 % ( w / w ) to about 0.3 % ( w /w ), or from about 0.3 % ( w / w )
to about 0.4 % ( w / w ), or from about 0.4 % ( w / w ) to about
0.5 % ( w / w ), or from about 0.5 % ( w / w ) to about 0.6 % ( w / w ),
or from about 0.6 % ( w / w ) to about 0.7 % ( w / w ), or from
60 about 0.7 % ( w / w ) to about 0.8 % ( w / w ), or from about 0.8 %
?? ( w / w ) to about 0.9 % ( w / w ), or from about 0.9 % ( w / w ) to
H about 1 % ( w / w ), or from about 1 % ( w / w ) to about 5 %
HO
( w / w ), or from about 5 % ( w /w ) to about 10 % ( w / w ), or from
about 10 % ( w / w ) to about 15 % ( w / w ), or from about 15 %
( 5aS , 6S , 9R , 9aR ) -cannabielsoic acid A 65 ( w / w ) to about 20 % ( w / w ), or from about 20% ( w / w ) to
[ CBEA A] about 25 % ( w / w ), or from about 25 % ( w / w ) to about 30 %
( w / w ), or from about 30 % ( w / w ) to about 35 % ( w / w ), or
 US 10,806,707 B2
31 32
from about 35 % ( w / w ) to about 40% ( w / w ), or from about embodiments, THC is present in an amount of about 1 , 2 , 3 ,
40 % ( w / w ) to about 45 % ( w / w ), or from about 45 % ( w / w ) 4 , 5 , 6 , or 7 % ( w / w ). In some embodiments, oils containing
to about 50 % ( w / w ), or from about 50 % ( w / w ) to about 55 % about 1-7 % ( w / w ) THC (or other ranges between about 1 %
( w / w ), or from about 55 % ( w / w ) to about 60% ( w / w ), or and about 7 % ) are prepared using the AC/ DC cannabis
from about 60 % ( w / w ) to about 65 % ( w / w ), or from about 5 strain .
65 % ( w / w ) to about 70 % ( w / w ), or from about 70% ( w / w ) In some embodiments, THC is present in an amount
to about 75 % ( w / w ), or from about 75 % ( w / w ) to about 80 % ranging from about 15 % ( w / w ) to about 18 % ( w / w ), or from
( w / w ), or from about 80 % ( w / w ) to about 85 % ( w / w ), or about 18 % ( w / w ) to about 21 % ( w / w ), or from about 21 %
from about 85 % ( w / w ) to about 90% ( w / w ), or from about ( w / w ) to about 24 % ( w / w ), or from about 24 % ( w / w ) to
90 % ( w / w ) to about 95 % ( w / w ), or from about 95 % ( w / w ) 10 about 27 % ( w / w ), or from about 27 % ( w / w ) to about 30 %
to about 99 % ( w /w ). ( w / w ), or from about 30 % ( w / w ) to about 33 % ( w / w ), or
A neutral cannabinoid ( such as THC , CBD , CBG , CBN , from about 33 % ( w / w ) to about 36 % ( w /w ), or from about
or another neutral cannabinoid ) can be present in an amount 36 % ( w / w ) to about 39 % ( w / w ), or from about 39 % ( w / w )
ranging from about 0.01 % ( w / w ) to about 1 % ( w / w ), or from to about 42 % ( w / w ), or from about 42 % ( w / w ) to about 45 %
about 0.02 % ( w / w ) to about 0.9 % ( w / w ), or from about 15 ( w / w ). In some embodiments, THC is present in an amount
0.03 % ( w / w ) to about 0.8 % ( w / w ), or from about 0.04 % ranging from about 15 % ( w / w ) to about 45 % ( w / w ), or from
( w / w ) to about 0.7 % ( w / w ), or from about 0.05 % ( w / w ) to about 18 % ( w / w ) to about 42 % ( w / w ), or from about 21 %
about 0.6 % ( w / w ), or from about 0.06 % ( w / w ) to about 0.5 % ( w / w ) to about 39 % ( w / w ), or from about 24 % ( w / w ) to
( w / w ), or from about 0.07 % ( w / w ) to about 0.4 % ( w / w ), or about 36 % ( w / w ), or from about 27 % ( w / w ) to about 33 %
from about 0.08 % ( w / w ) to about 0.3 % ( w / w ), or from about 20 ( w / w ). In some embodiments , THC is present in an amount
0.09 % ( w / w ) to about 0.2 % ( w /w ). A neutral cannabinoid of about 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ,
( such as THC , CBD , CBG , CBN , or another neutral can 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 ,
nabinoid ) can be present in an amount ranging from about or 45 % ( w / w ). In some embodiments, oils containing about
1 % ( w /w ) to about 10 % ( w / w ), or from about 2 % ( w / w ) to 15-45 % ( w / w ) THC ( or other ranges between about 15 % and
about 9 % ( w / w ), or from about 3 % ( w / w ) to about 8 % 25 about 45 % ) is prepared using a cannabis strain selected from
( w / w ), or from about 4 % ( w / w ) to about 7 % ( w / w ), or from Buddha Passion , Cannatonic , Medihaze, Harle OG , Harle
about 5 % ( w / w ) to about 6 % ( w / w ). A neutral cannabinoid Tsu , Hopesprings , Elektra, and Harlequin . In some embodi
( such as THC , CBD , CBG , CBN , or another neutral can ments, oils containing about 15-45 % ( w / w ) THC (or other
nabinoid ) can be present in an amount ranging from about ranges between about 15 % and about 45 % ) are prepared
5 % ( w / w ) to about 99 % ( w / w ), or from about 10 % ( w / w ) to 30 using the Cannatonic cannabis strain .
about 95 % ( w / w ), or from about 15 % ( w / w ) to about 90 % In some embodiments, THC is present in an amount
( w / w ), or from about 20 % ( w / w ) to about 85 % ( w / w ), or ranging from about 50 % ( w / w ) to about 53 % ( w / w ), or from
from about 25 % ( w / w ) to about 80% ( w / w ), or from about about 53 % ( w / w ) to about 56 % ( w / w ), or from about 56 %
30 % ( w / w ) to about 75 % ( w / w ), or from about 35 % ( w / w ) ( w / w ) to about 60 % ( w / w ), or from about 60% ( w / w ) to
to about 70 % ( w / w ), or from about 40 % ( w / w ) to about 65 % 35 about 63 % ( w / w ), or from about 63 % ( w / w ) to about 66 %
( w / w ), or from about 45 % ( w / w ) to about 60% ( w / w ), or ( w / w ), or from about 66 % ( w / w ) to about 69 % ( w / w ), or
from about 50% ( w / w ) to about 55 % ( w / w ). from about 69 % ( w / w ) to about 72 % ( w / w ), or from about
Typically, THC will be present in an oil of the invention 72 % ( w / w ) to about 75 % ( w / w ), or from about 75 % ( w / w )
in an amount ranging from about 1 % ( w / w ) to about 95 % to about 78 % ( w / w ), or from about 78 % ( w / w ) to about 81 %
( w / w ). Typically , THC is present in an amount ranging from 40 ( w / w ), or from about 81 % ( w /w ) to about 84 % ( w / w ), or
about 10 % ( w / w ) to about 95 % ( w / w ). THC can be present, from about 84 % ( w / w ) to about 87 % ( w / w ), or from about
for example, in an amount ranging from about 1 % ( w / w ) to 87 % ( w / w ) to about 90% ( w / w ). In some embodiments ,
about 5 % ( w / w ), or from about 5 % ( w / w ) to about 10 % THC is present in an amount ranging from about 50 % ( w / w )
( w / w ), or from about 10 % ( w / w ) to about 15 % ( w / w ), or to about 90% ( w / w ), or from about 55 % ( w / w ) to about 87 %
from about 15 % ( w / w ) to about 20% ( w / w ), or from about 45 ( w / w ), or from about 66 % ( w / w ) to about 84 % ( w / w ), or
20 % ( w / w ) to about 25 % ( w / w ), or from about 25 % ( w / w ) from about 69 % ( w / w ) to about 81 % ( w / w ), or from about
to about 30 % ( w / w ), or from about 30 % ( w / w ) to about 35 % 72 % ( w / w ) to about 78 % ( w / w ). In some embodiments,
( w / w ), or from about 35 % ( w / w ) to about 40% ( w / w ), or THC is present in an amount of about 50 , 51 , 52 , 53 , 54 , 55 ,
from about 40 % ( w / w ) to about 45 % ( w / w ), or from about 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ,
45 % ( w / w ) to about 50 % ( w / w ), or from about 50% ( w / w ) 50 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 ,
to about 55 % ( w / w ), or from about 55 % ( w / w ) to about 60 % 88 , 89 , or 90% ( w / w ). In some embodiments, oils containing
( w / w ), or from about 60 % ( w /w ) to about 65 % ( w / w ), or about 50-90 % ( w / w ) THC (or other ranges between about
from about 65 % ( w / w ) to about 70% ( w / w ), or from about 50% and about 90 % ) are prepared using a cannabis strain
70 % ( w / w ) to about 75 % ( w / w ), or from about 75 % ( w / w ) selected from Blueberry, Afghan Goo , Infinite Euphoria ,
to about 80 % ( w / w ), or from about 80 % ( w / w ) to about 85 % 55 Snowcap , Blackberry Kush , Sour Kush , Blue Diesel , and
( w / w ), or from about 85 % ( w / w ) to about 90% ( w / w ), or Prize Kush . In some embodiments, oils containing about
from about 90 % ( w / w ) to about 95 % ( w /w ). THC can be 50-90% ( w / w ) THC ( or other ranges between about 50% and
present in an amount ranging from about 5 % ( w / w ) to about about 90% ) are prepared using the Blueberry cannabis
95 % ( w / w ), or from about 10 % ( w / w ) to about 90 % ( w / w ), strain .
or from about 15 % ( w / w ) to about 85 % ( w / w ), or from about 60 Typically , CBD will be present in an oil of the invention
20 % ( w / w ) to about 80 % ( w / w ), or from about 25 % ( w / w ) in an amount ranging from about 0.1 % ( w / w ) to about 99 %
to about 75 % ( w / w ), or from about 30 % ( w / w ) to about 70 % ( w / w ). In some embodiments, CBD is present in an amount
( w /w ), or from about 35 % ( w / w ) to about 65 % ( w / w ), or ranging from about 0.1 % ( w / w ) to about 80 % ( w / w ). CBD
from about 40 % ( w / w ) to about 60% ( w / w ), or from about can be present, for example, in an amount ranging from
45 % ( w / w ) to about 55 % ( w / w ). 65 about 0.1 % ( w / w ) to about 1 % ( w / w ), or from about 1 %
In some embodiments, THC is present in an amount ( w / w ) to about 5 % ( w / w ), or from about 5 % ( w / w ) to about
ranging from about 2 % ( w / w ) to about 4 % ( w / w ). In some 10% ( w / w ), or from about 10 % ( w / w ) to about 15 % ( w / w ),
 US 10,806,707 B2
33 34
or from about 15 % ( w / w ) to about 20 % ( w / w ), or from about from about 60 % ( w / w ) to about 65 % ( w / w ), or from about
20 % ( w / w ) to about 25 % ( w / w ), or from about 25 % ( w / w ) 65 % ( w / w ) to about 70 % ( w / w ), or from about 70 % ( w / w )
to about 30 % ( w / w ), or from about 30 % ( w / w ) to about 35 % to about 75 % ( w / w ), or from about 75 % ( w / w ) to about 80 %
( w / w ), or from about 35 % ( w / w ) to about 40% ( w / w ), or ( w / w ). In some embodiments , CBD is present in an amount
from about 40 % ( w / w ) to about 45 % ( w / w ), or from about 5 from about 50 % ( w / w ) to about 80 % ( w / w ), or from about
45 % ( w / w ) to about 50 % ( w / w ), or from about 50% ( w / w ) 53 % ( w / w ) to about 77 % ( w / w ), or from about 56 % ( w / w )
to about 55 % ( w / w ), or from about 55 % ( w / w ) to about 60 % to about 74 % ( w / w ), or from about 59 % ( w / w ) to about 71 %
( w / w ), or from about 60 % ( w / w ) to about 65 % ( w / w ), or ( w / w ), or from about 62 % ( w / w ) to about 68 % ( w / w ). In
from about 65 % ( w / w ) to about 70% ( w / w ), or from about some embodiments, CBD is present in an amount of about
70 % ( w / w ) to about 75 % ( w / w ), or from about 75 % ( w / w ) 10 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 ,
to about 80 % ( w / w ). CBD can be present, for example , in an 67 , 68 , 69 , or 70 % ( w / w ). In some embodiments, oils
amount ranging from about 5 % ( w / w ) to about 80 % ( w / w ), containing about 50-80% ( w / w ) CBD (or other ranges
or from about 10 % ( w / w ) to about 75 % ( w / w ), or from about between about 50 % and about 80 % ) are prepared using a
15 % ( w / w ) to about 70 % ( w / w ), or from about 20% ( w / w ) cannabis strain selected from Cannatonic , Harle OG , Harle
to about 65 % ( w / w ), or from about 25 % ( w / w ) to about 60 % 15 Tsu , and AC/DC . In some embodiments, oils containing
( w / w ), or from about 30 % ( w / w ) to about 55 % ( w / w ), or about 50-80% ( w / w ) CBD (or other ranges between about
from about 35 % ( w / w ) to about 50% ( w / w ). 50% and about 80 % ) are prepared using the AC/DC can
In some embodiments , CBD is present in an amount nabis strain .
ranging from about 0.1 % ( w / w ) to about 0.2 % ( w / w ), or Typically , CBG will be present in an oil of the invention
from about 0.2 % ( w / w ) to about 0.6 % ( w / w ), or from about 20 in an amount ranging from about 0.1 % ( w / w ) to about 80 %
0.6 % ( w / w ) to about 1 % ( w / w ), or from about 1 % ( w / w ) to ( w / w ). In some embodiments , CBG is present in an amount
about 1.4 % ( w / w ), or from about 1.4 % ( w / w ) to about 1.8 % ranging from about 0.1 % ( w / w ) to about 0.2 % ( w / w ), or
( w / w ), or from about 1.8 % ( w / w ) to about 2.2 % ( w / w ), or from about 0.2 % ( w /w ) to about 0.6 % ( w /w ), or from about
from about 2.2 % ( w / w ) to about 2.6 % ( w / w ), or from about 0.6 % ( w / w ) to about 1 % ( w / w ), or from about 1 % ( w / w ) to
2.6 % ( w / w ) to about 3 % ( w / w ), or from about 3 % ( w / w ) to 25 about 1.4 % ( w / w ), or from about 1.4 % ( w / w ) to about 1.8 %
about 3.4 % ( w / w ), or from about 3.4 % ( w / w ) to about 3.8 % ( w / w ), or from about 1.8 % ( w / w ) to about 2.2 % ( w / w ), or
( w / w ), or from about 3.8 % ( w /w ) to about 4.2 % ( w / w ), or from about 2.2 % ( w / w ) to about 2.6 % ( w / w ), or from about
from about 4.2 % ( w / w ) to about 4.6 % ( w / w ), or from about 2.6 % ( w / w ) to about 3 % ( w / w ), or from about 3 % ( w / w ) to
4.6 % ( w / w ) to about 5 % ( w / w ). In some embodiments , CBD about 3.4 % ( w / w ), or from about 3.4 % ( w / w ) to about 3.8 %
is present in an amount ranging from about 0.2 % ( w / w ) to 30 ( w / w ), or from about 3.8 % ( w / w ) to about 4.2 % ( w / w ), or
about 5 % ( w / w ), or from about 0.6 % ( w / w ) to about 4.6 % from about 4.2 % ( w /w ) to about 4.6 % ( w /w ), or from about
( w / w ), or from about 1 % ( w / w ) to about 4.2 % ( w / w ), or 4.6 % ( w / w ) to about 5 % ( w / w ). In some embodiments, CBG
from about 1.4 % ( w /w ) to about 3.8 % ( w /w ), or from about is present in an amo nt ranging from about 0.2 % ( w / w ) to
1.8 % ( w / w ) to about 3.4 % ( w / w ), or from about 2.2 % ( w / w ) about 5 % ( w / w ), or from about 0.6 % ( w / w ) to about 4.6 %
to about 3 % ( w / w ). In some embodiments , CBD is present 35 ( w / w ), or from about 1 % ( w / w ) to about 4.2 % ( w / w ), or
in an amount of about 0.1 , 0.2 , 0.4 , 0.6 , 0.8 , 1 , 1.2 , 1.4 , 1.6 , from about 1.4 % ( w / w ) to about 3.8 % ( w / w ), or from about
1.8 , 2 , 2.2 , 2.4 , 2.6 , 2.8 , or 3 % ( w / w ). In some embodiments, 1.8 % ( w / w ) to about 3.4 % ( w / w ), or from about 2.2 % ( w / w )
oils containing about 0.1-5 % ( w / w ) CBD (or other ranges to about 3 % ( w / w ). In some embodiments, CBG is present
between about 0.1 % and about 5 % ) are prepared using a in an amount of about 0.1,0.2 , 0.4 , 0.6 , 0.8 , 1 , 1.2 , 1.4 , 1.6 ,
cannabis strain selected from Blueberry and Prize Cush . In 40 1.8 , 2 , 2.2 , 2.4 , 2.6 , 2.8 , or 3 % ( w / w ). In some embodiments ,
some embodiments, oils containing about 0.1-5 % ( w / w ) oils containing about 0.1-5 % ( w / w ) CBG (or other ranges
CBD ( or other ranges between about 0.1 % and about 5 % ) between about 0.1 % and about 5 % ) are prepared using a
are prepared using the Blueberry cannabis strain . cannabis strain selected from AC/DC , Cannatonic , and
In some embodiments, CBD is present in an amount Blueberry.
ranging from about 25 % ( w / w ) to about 30% ( w / w ), or from 45 In general, acidic cannabinoids ( such as THCA , CBDA ,
about 30 % ( w / w ) to about 35 % ( w / w ), or from about 35 % CBGA, and other acidic cannabinoids) are present in the oils
( w / w ) to about 40 % ( w / w ), or from about 40 % ( w / w ) to of the present invention ( e.g. , cannabis oil extracts or
about 45 % ( w / w ), or from about 45 % ( w / w ) to about 50 % compositions comprising same) in amounts ranging from
( w / w ), or from about 50 % ( w / w ) to about 55 % ( w / w ). In about 0.001 % ( w / w ) to about 80 % ( w / w ). In some embodi
some embodiments, CBD is present in an amount ranging 50 ments, an acidic cannabinoid ( such as THCA , CBDA ,
from about 25 % ( w / w ) to about 55 % ( w / w ), or from about CBGA , or another acidic cannabinoid ) will be present in an
28 % ( w / w ) to about 52 % ( w / w ), or from about 31 % ( w /w ) amount ranging from about 0.001 % ( w / w ) to about 50 %
to about 49 % ( w / w ), or from about 34 % ( w / w ) to about 46 % ( w /w ). An acidic cannabinoid ( such as THCA , CBDA ,
( w / w ), or from about 37 % ( w / w ) to about 43 % ( w / w ). In CBGA, or another acidic cannabinoid ) can be present, for
some embodiments, CBD is present in an amount of about 55 example, in an amount ranging from about 0.01 % ( w / w ) to
30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , about 0.05 % ( w / w ), or from about 0.05 % ( w / w ) to about
46 , 47 , 48 , 49 , or 50% ( w / w ). In some embodiments, oils 0.1 % ( w / w ), or from about 0.1 % ( w / w ) to about 0.2 % ( w / w ),
containing about 25-55 % ( w / w ) CBD (or other ranges or from about 0.2 % ( w / w ) to about 0.3 % ( w / w ), or from
between about 25 % and about 55 % ) are prepared using a about 0.3 % ( w / w ) to about 0.4 % ( w / w ), or from about 0.4 %
cannabis strain selected from Cannatonic , Medihaze , and 60 ( w / w ) to about 0.5 % ( w / w ), or from about 0.5 % ( w / w ) to
Harlequin . In some embodiments, oils containing about about 0.6 % ( w / w ), or from about 0.6 % ( w / w ) to about 0.7 %
25-55 % ( w / w ) CBD ( or other ranges between about 25 % ( w / w ), or from about 0.7 % ( w / w ) to about 0.8 % ( w / w ), or
and about 55 % ) are prepared using the Cannatonic cannabis from about 0.8 % ( w / w ) to about 0.9 % ( w / w ), or from about
strain . 0.9 % ( w / w ) to about 1 % ( w / w ), or from about 1 % ( w / w ) to
In some embodiments, CBD is present in an amount 65 about 5 % ( w / w ), or from about 5 % ( w / w ) to about 10 %
ranging from about from about 50 % ( w / w ) to about 55 % ( w / w ), or from about 10 % ( w / w ) to about 15 % ( w / w ), or
( w / w ), or from about 55 % ( w / w ) to about 60% ( w / w ), or from about 15 % ( w / w ) to about 20 % ( w / w ), or from about
 US 10,806,707 B2
35 36
20 % ( w /w ) to about 25 % ( w / w ), or from about 25 % ( w / w ) octahydroazulen - 5 -yl ]propan - 2 -ol ]; a -humulene [ ( 1E , 4E ,
to about 30 % ( w / w ), or from about 30 % ( w / w ) to about 35 % 8E )-2,6,6,9 - tetramethylcycloundeca - 1,4,8 - triene ];
( w / w ), or from about 35 % ( w / w ) to about 40% ( w / w ), or B -humulene [(1E ,5E ) -1,4,4 -trimethyl-8 -methylidenecy
from about 40 % ( w / w ) to about 45 % ( w / w ), or from about cloundeca - 1,5 -diene ]; y -humulene [(1Z,6E ) -1,8,8 -trimethyl
45 % ( w / w ) to about 50% ( w / w ), or from about 50% ( w / w ) 5 5 -methylidenecycloundeca - 1,6 - diene); D - limonene [(4R )-1
to about 55 % ( w / w ), or from about 55 % ( w / w ) to about 60 % methyl - 4 -prop - 1 -en - 2 -ylcyclohexene ); L - limonene [( 4S ) -1
( w / w ), or from about 60 % ( w /w ) to about 65 % ( w / w ), or methyl - 4 -prop - 1 - en - 2 -ylcyclohexene ]; ( - ) - linalool [ ( 3R ) -3 ,
from about 65 % ( w / w ) to about 70% ( w / w ), or from about 7 -dimethylocta -1,6 -dien - 3 -ol ]; ( + ) - linalool [ ( 3S ) -3,7
70 % ( w / w ) to about 75 % ( w / w ), or from about 75 % ( w / w ) dimethylocta - 1,6 -dien - 3 -ol ]; C -myrcene [2 -methyl-6
to about 80 % ( w / w ). 10 methylideneocta - 1,7 -diene ]; B -myrcene [ 7 -methyl- 3
An acidic cannabinoid ( such as THCA , CBDA , CBGA , or methylideneocta - 1,6 -diene ); nerol [(2Z )-3,7 -dimethylocta
another acidic cannabinoid ) can be present in an amount 2,6 - dien - 1 - ol ] ; cis -nerolidol [(6Z )-3,7,11- trimethyldodeca
ranging from about 0.01 % ( w / w ) to about 1 % ( w / w ), or from 1,6,10 -trien - 3 -ol ]; trans -nerolidol [ ( CE ) -3,7,11
about 0.02 % ( w / w ) to about 0.9 % ( w / w ), or from about trimethyldodeca - 1,6,10 - trien - 3 -ol ]; a - ocimene [(3E )-3,7
0.03 % ( w / w ) to about 0.8 % ( w / w ), or from about 0.04 % 15 dimethylocta - 1,3,7 - triene]; B - ocimene | (3E ) -3,7
( w / w ) to about 0.7 % ( w / w ), or from about 0.05 % ( w / w ) to dimethylocta - 1,3,6 - triene ]; p - cymene [ 1 -methyl - 4-1
about 0.6 % ( w / w ), or from about 0.06 % ( w / w ) to about 0.5 % methylethyl )benzene); a -phellandrene [2-methyl-5-propan
( w / w ), or from about 0.07 % ( w / w ) to about 0.4 % ( w / w ), or 2 -ylcyclohexa - 1,3 -diene ); B -phellandrene [3 -methylidene
from about 0.08 % ( w / w ) to about 0.3 % ( w / w ), or from about 6 -propan - 2 -ylcyclohexene ); cis -phytol [ ( Z , 7R , 11R) -3,7,11 ,
0.09% ( w / w ) to about 0.2 % ( w / w ). An acidic cannabinoid 20 15 -tetramethylhexadec -2 -en -1-ol]; trans-phytol [ (E , 7R,
( such as THCA , CBDA , CBGA , or another acidic cannabi 11R )-3,7,11,15 - tetramethylhexadec - 2 - en - 1 -ol]; ( - )- a
noid) can be present in an amount ranging from about 1 % pinene [ (15,5S )-4,6,6 - trimethylbicyclo [3.1.1 ]hept - 3 - ene ];
( w / w ) to about 10 % ( w / w ), or from about 2 % ( w / w ) to about ( - ) - 3 -pinene [ ( 18,5S ) -6,6 -dimethyl - 4 -methylidenebicyclo
9 % ( w / w ), or from about 3 % ( w / w ) to about 8 % ( w / w ), or [3.1.1 ]heptane ); ( + ) - a -pinene [(1R ,5R )-4,6,6 - trimethylbicy
from about 4 % ( w /w ) to about 7 % ( w / w ), or from about 5 % 25 clo [ 3.1.1 ] hept - 3 - ene) ; ( + ) - 3 - pinene [(1R ,5R ) -6,6 -dimethyl
( w / w ) to about 6 % ( w / w ). 4 -methylidenebicyclo [ 3.1.1 ]heptane ]; ( - ) -pulegone [(5S )-5
An acidic cannabinoid ( such as THCA , CBDA , CBGA , or methyl - 2 -propan - 2 -ylidenecyclohexan - 1 - one ]; (+)
another acidic cannabinoid ) can be present in an amount pulegone [(5R )-5 -methyl- 2 -propan - 2 -ylidenecyclohexan -1
ranging from about 5 % ( w / w ) to about 50 % ( w / w ), or from one ); C -terpinene [1-methyl-4-propan -2-ylcyclohexa - 1,3
about 8 % ( w /w ) to about 48 % ( w / w ), or from about 10 % 30 diene ); 6 - terpinene [5 -methyl-2 -propan - 2-ylcyclohexa- 1,3
( w / w ) to about 46 % ( w / w ), or from about 12 % ( w / w ) to diene ); y - terpinene [1 -methyl-4 -propan - 2- ylcyclohexa - 1,4
about 44 % ( w / w ), or from about 14 % ( w / w ) to about 42 % diene ); a - terpineol [ 2- (4 -methylcyclohex -3 -en - 1 -yl)propan
( w / w ), or from about 16 % ( w / w ) to about 40% ( w / w ), or 2 - ol ] ; y -terpineol [ 1-methyl-4 - propan -2 -ylidenecyclohexan
from about 18 % ( w / w ) to about 38 % ( w / w ), or from about 1 - ol ] ; ( + ) -valencene [( 3R ,4S,5R )-4a ,5 -dimethyl-3 -prop - 1
20 % ( w / w ) to about 36 % ( w / w ), or from about 22 % ( w / w ) 35 en - 2 -yl-2,3,4,5,6,7 -hexahydro - 1H -naphthalene); and
to about 34 % ( w / w ), or from about 24 % ( w / w ) to about 32 % combinations thereof.
( w / w ), or from about 26 % ( w / w ) to about 30% ( w / w ). An In some embodiments, the invention provides an oil
acidic cannabinoid ( such as THCA , CBDA , CBGA , or containing one more terpenes selected from B -myrcene,
another acidic cannabinoid ) can be present in an amount linalool, a - terpineol, ß - caryophyllene, B -caryophyllene
ranging from about 50 % ( w / w ) to about 75 % ( w / w ), or from 40 oxide, a - humulene, valencene , cis -nerolidol, guaiol ,
about 52 % ( w / w ) to about 74 % ( w / w ), or from about 54 % a - eudesmol, B - eudesmol, y -eudesmol, and a -bisabolol . In
( w / w ) to about 72 % ( w / w ), or from about 56 % ( w / w ) to some embodiments, the oil contains one or more terpenes
about 70 % ( w / w ), or from about 58 % ( w / w ) to about 68 % selected from linalool , ß -caryophyllene, ß -caryophyllene
( w / w ), or from about 60 % ( w / w ) to about 66 % ( w / w ), or oxide, a -humulene, cis -nerolidol, guaiol , a -eudesmol, and
from about 62 % ( w / w ) to about 64 % ( w / w ). 45 a - bisabolol.
A. Terpenes In general, terpenes are present in the oils of the invention
As noted above, cannabis oils of the present invention in total amounts ranging from about 0.1 % ( w / w ) to about
( e.g. , cannabis oil extracts or compositions comprising 15 % ( w / w ). For example , the total terpene content can range
same) generally contain at least one terpene compound. from about 0.1 % ( w / w ) to about 0.2 % ( w / w ), or from about
Terpenes are hydrocarbon compounds having carbon skel- 50 0.2 % ( w / w ) to about 0.3 % ( w / w ), or from about 0.3 % ( w / w )
etons derived from isoprene ( i.e. , CH2 =C ( CH ) 2CH=CH2 ) . to about 0.4 % ( w / w ), or from about 0.4 % ( w / w ) to about
Carbon atoms in the terpene backbone can bear oxygen 0.5 % ( w / w ), or from about 0.5 % ( w / w ) to about 0.6 % ( w / w ),
substituents such as hydroxyl, oxo , and carboxy groups . or from about 0.6 % ( w / w ) to about 0.7 % ( w / w ), or from
Terpenes present in the cannabis oils of the invention about 0.7 % ( w / w ) to about 0.8 % ( w / w ), or from about 0.8 %
include , but are not limited to , C? hemiterpenes, C10 55 ( w / w ) to about 0.9 % ( w / w ), or from about 0.9 % ( w /w ) to
monoterpenes , Cis sesquiterpenes, C20 diterpenes , and com about 1 % ( w / w ). The total terpene content can range from
binations thereof. Examples of terpenes include, but are not about 1 % ( w / w ) to about 2 % ( w / w ), or from about 2 % ( w / w )
limited to : ß -caryophyllene [( 1R ,40,9S )-4,11,11- trimethyl to about 3 % ( w / w ), or from about 3 % ( w / w ) to about 4 %
8 -methylene- bicyclo (7.2.0 ) undec - 4 -ene ]; B -caryophyllene ( w / w ), or from about 4 % ( w / w ) to about 5 % ( w / w ), or from
oxide ; citronellol [ 3,7 - dimethyl -6 - octen - 1 -ol ]; a - eudesmol 60 about 5 % ( w / w ) to about 6 % ( w / w ), or from about 6 % ( w / w )
[ 2- [(2R , 4aR )-4a, 8- dimethyl- 2,3,4,5,6,8a -hexahydro - 1H to about 7 % ( w / w ), or from about 7 % ( w / w ) to about 8 %
naphthalen -2 -yl]propan -2 - ol ]; B - eudesmol [ 2 - [ ( 2R , 4aR , ( w / w ), or from about 8 % ( w / w ) to about 9 % ( w / w ), or from
8aS ) -4a -methyl-8 -methylidene - 1,2,3,4,5,6,7,8a -octahy about 9 % ( w / w ) to about 10% ( w / w ), or from about 10 %
dronaphthalen - 2 - yl ) propan - 2 -ol ]; y - eudesmol [2 -[(2R ,4aR ) ( w / w ) to about 11 % ( w / w ), or from about 11 % ( w / w ) to
4a, 8 -dimethyl-2,3,4,5,6,7 -hexahydro -1H -naphthalen - 2 -yl) 65 about 12 % ( w / w ), or from about 12 % ( w / w ) to about 13 %
propan - 2 - ol ] ; geraniol [( 2E ) -3,7 - dimethylocta -2,6 - dien - 1 ( w / w ), or from about 13 % ( w / w ) to about 14 % ( w / w ), or
ol ] ; guaiol [ 2 -[ (3S,5R ,8S )-3,8 - dimethyl- 1,2,3,4,5,6,7,8 from about 14 % ( w / w ) to about 15 % ( w / w ).
 US 10,806,707 B2
37 38
In some embodiments, the invention provides oils with ranging from about 0.9 % ( w / w ) to about 1.2 % ( w / w ), or
total terpene content ranging from about 0.1 % ( w / w ) to from about 1.2 % ( w / w ) to about 1.5 % ( w / w ), or from about
about 1 % ( w / w ), or from about 0.2 % ( w / w ) to about 0.9 % 1.5 % ( w / w ) to about 1.8 % ( w / w ), or from about 1.8 % ( w / w )
( w / w ), or from about 0.3 % ( w / w ) to about 0.8 % ( w / w ), or to about 2.1 % ( w / w ), or from about 2.1 % ( w / w ) to about
from about 0.4 % ( w / w ) to about 0.7 % ( w / w ), or from about 5 2.4 % ( w / w ), or from about 2.4 % ( w / w ) to about 2.7 % ( w / w ),
0.5 % ( w / w ) to about 0.6 % ( w / w ). In some embodiments, the or from about 2.7 % ( w / w ) to about 3 % ( w / w ).
total terpene content ranges from about 1 % ( w / w ) to about In some embodiments, the invention provides oils
15 % ( w / w ), or from about 2 % ( w / w ) to about 13 % ( w / w ), or wherein ß - caryophyllene oxide is present in an amount
from about 3 % ( w / w ) to about 11 % ( w / w ), or from about 4 % ranging from about 0.1 % ( w / w ) to about 0.2 % ( w / w ), or
( w / w ) to about 9 % ( w / w ), or from about 5 % ( w / w ) to about 10 from about 0.2 % ( w / w ) to about 0.3 % ( w / w ), or from about
7 % ( w /w ). 0.3 % ( w / w ) to about 0.4 % ( w / w ). In some embodiments, the
In some embodiments, the invention provides oils invention provides oils wherein ß -caryophyllene oxide is
wherein linalool is present in an amount ranging from about present in an amount ranging from about 0.4 % ( w / w ) to
0 % ( w / w ) to about 0.1 % ( w / w ), or from about 0.1 % ( w / w ) about 0.6 % ( w / w ), or from about 0.6 % ( w / w ) to about 0.9 %
to about 0.2 % ( w / w ), or from about 0.2 % ( w / w ) to about 15 ( w / w ), or from about 0.9 % ( w / w ) to about 1.2 % ( w / w ), or
0.3 % ( w / w ), or from about 0.3 % ( w / w ) to about 0.4 % ( w / w ). from about 1.2 % ( w / w ) to about 1.5 % ( w / w ), or from about
In some embodiments, the invention provides oils wherein 1.5 % ( w / w ) to about 1.8 % ( w / w ), or from about 1.8 % ( w / w )
linalool is present in an amount ranging from about 0.4 % to about 2.1 % ( w / w ), or from about 2.1 % ( w / w ) to about
( w / w ) to about 0.6 % ( w / w ), or from about 0.6 % ( w / w ) to 2.4 % ( w / w ), or from about 2.4 % ( w / w ) to about 2.7 % ( w / w ),
about 0.9 % ( w / w ), or from about 0.9 % ( w / w ) to about 1.2 % 20 or from about 2.7 % ( w / w ) to about 3 % ( w / w ).
( w / w ), or from about 1.2 % ( w / w ) to about 1.5 % ( w / w ), or In some embodiments, the invention provides oils
from about 1.5 % ( w / w ) to about 1.8 % ( w / w ), or from about wherein guaiol is present in an amount ranging from about
1.8 % ( w / w ) to about 2.1 % ( w / w ), or from about 2.1 % ( w / w ) 0.0 % ( w / w ) to about 0.1 % ( w / w ), or from about 0.1 % ( w / w )
to about 2.4 % ( w / w ), or from about 2.4 % ( w / w ) to about to about 0.2 % ( w / w ), or from about 0.2 % ( w / w ) to about
2.7 % ( w / w ), or from about 2.7 % ( w / w ) to about 3 % ( w / w ). 25 0.3 % ( w / w ), or from about 0.3 % ( w / w ) to about 0.4 % ( w / w ),
In some embodiments, the invention provides oils or from about 0.4 % ( w / w ) to about 0.5 % ( w / w ), or from
wherein ß -caryophyllene is present in an amount ranging about 0.5 % ( w / w ) to about 0.6 % ( w / w ), or from about 0.6 %
from about 0.2 % ( w /w ) to about 0.3 % ( w /w ), or from about ( w / w ) to about 0.7 % ( w / w ). In some embodiments, the
0.3 % ( w / w ) to about 0.4 % ( w / w ), or from about 0.4 % ( w / w ) invention provides oils wherein guaiol is present in an
to about 0.5 % ( w / w ), or from about 0.5 % ( w / w ) to about 30 amount ranging from about 0.7 % ( w / w ) to about 0.9 %
0.6 % ( w / w ), or from about 0.6 % ( w / w ) to about 0.7 % ( w / w ), ( w / w ), or from about 0.9 % ( w / w ) to about 1.2 % ( w / w ), or
or from about 0.7 % ( w /w ) to about 0.8 % ( w / w ), or from from about 1.2 % ( w / w ) to about 1.5 % ( w / w ), or from about
about 0.8 % ( w / w ) to about 0.9 % ( w / w ), or from about 0.9 % 1.5 % ( w / w ) to about 1.8 % ( w / w ), or from about 1.8 % ( w / w )
( w / w ) to about 1.0% ( w / w ), or from about 1.0% ( w / w ) to to about 2.1 % ( w /w ), or from about 2.1 % ( w / w ) to about
about 1.1 % ( w / w ). In some embodiments , the invention 35 2.4 % ( w / w ), or from about 2.4 % ( w / w ) to about 2.7 % ( w / w ),
provides oils wherein ß - caryophyllene is present in an or from about 2.7 % ( w / w ) to about 3 % ( w / w ).
amount ranging from about 1.1 % ( w / w ) to about 1.5 % In some embodiments , the invention provides oils
( w / w ), or from about 1.5 % ( w / w ) to about 1.8 % ( w / w ), or wherein B -eudesmol is present in an amount ranging from
from about 1.8 % ( w / w ) to about 2.1 % ( w / w ), or from about about 0.0% ( w / w ) to about 0.1 % ( w / w ), or from about 0.1 %
2.1 % ( w / w ) to about 2.4 % ( w / w ), or from about 2.4 % ( w / w ) 40 ( w / w ) to about 0.2 % ( w / w ), or from about 0.2 % ( w / w ) to
to about 2.7 % ( w / w ), or from about 2.7 % ( w / w ) to about 3 % about 0.3 % ( w / w ), or from about 0.3 % ( w / w ) to about 0.4 %
( w /w ). ( w / w ), or from about 0.4 % ( w / w ) to about 0.6 % ( w / w ). In
In some embodiments, the invention provides oils some embodiments, the invention provides oils wherein
wherein a -humulene is present in an amount ranging from B - eudesmol is present in an amount ranging from about
about 0.1 % ( w / w ) to about 0.2 % ( w / w ), or from about 0.2 % 45 0.6 % ( w / w ) to about 0.9 % ( w / w ), or from about 0.9 % ( w / w )
( w / w ) to about 0.3 % ( w / w ), or from about 0.3 % ( w / w ) to to about 1.2 % ( w / w ), or from about 1.2 % ( w / w ) to about
about 0.4 % ( w / w ), or from about 0.4 % ( w / w ) to about 0.5 % 1.5 % ( w / w ), or from about 1.5 % ( w / w ) to about 1.8 % ( w / w ),
( w / w ), or from about 0.5 % ( w / w ) to about 0.6 % ( w / w ). In or from about 1.8 % ( w / w ) to about 2.1 % ( w /w ), or from
some embodiments, the invention provides oils wherein about 2.1 % ( w / w ) to about 2.4 % ( w / w ), or from about 2.4 %
a -humulene is present in an amount ranging from about 50 ( w / w ) to about 2.7 % ( w / w ), or from about 2.7 % ( w / w ) to
0.6 % ( w / w ) to about 0.9 % ( w / w ), or from about 0.9 % ( w / w ) about 3% (w/w).
to about 1.2 % ( w / w ), or from about 1.2 % ( w / w ) to about In some embodiments, the invention provides oils
1.5 % ( w / w ), or from about 1.5 % ( w / w ) to about 1.8 % ( w / w ), wherein a -bisalobol is present in an amount ranging from
or from about 1.8 % ( w / w ) to about 2.1 % ( w / w ), or from about 0.0% ( w / w ) to about 0.1 % ( w / w ), or from about 0.1 %
about 2.1 % ( w / w ) to about 2.4 % ( w / w ), or from about 2.4 % 55 ( w / w ) to about 0.2 % ( w / w ), or from about 0.2 % ( w / w ) to
( w /w ) to about 2.7 % ( w /w ), or from about 2.7 % ( w / w ) to about 0.3 % ( w / w ), or from about 0.3 % ( w / w ) to about 0.4 %
about 3 % ( w / w ). ( w / w ), or from about 0.4 % ( w / w ) to about 0.5 % ( w / w ), or
In some embodiments , the invention provides oils from about 0.5 % ( w /w ) to about 0.6 % ( w /w ), or from about
wherein cis -nerolidol is present in an amount ranging from 0.6 % ( w / w ) to about 0.7 % ( w / w ), or from about 0.7 % ( w / w )
about 0.1 % ( w / w ) to about 0.2 % ( w / w ), or from about 0.2 % 60 to about 0.8 % ( w / w ), or from about 0.8 % ( w / w ) to about
( w / w ) to about 0.3 % ( w / w ), or from about 0.3 % ( w /w ) to 0.9 % ( w / w ), or from about 0.9 % ( w / w ) to about 1.0% ( w / w ),
about 0.4 % ( w / w ), or from about 0.4 % ( w / w ) to about 0.5 % or from about 1.0 % ( w / w ) to about 1.1 % ( w / w ). In some
( w /w ), or from about 0.5 % ( w / w ) to about 0.6 % ( w / w ), or embodiments , the invention provides oils wherein a -bisa
from about 0.6 % ( w / w ) to about 0.7 % ( w / w ), or from about lobol is present in an amount ranging from about 1.1 %
0.7 % ( w / w ) to about 0.8 % ( w / w ), or from about 0.8 % ( w / w ) 65 (w / w ) to about 1.5 % ( w / w ), or from about 1.5 % ( w / w ) to
to about 0.9 % ( w / w ). In some embodiments , the invention about 1.8 % ( w / w ), or from about 1.8 % ( w / w ) to about 2.1 %
provides oils wherein cis - nerolidol is present in an amount ( w / w ), or from about 2.1 % ( w / w ) to about 2.4 % ( w / w ), or
 US 10,806,707 B2
39 40
from about 2.4 % ( w / w ) to about 2.7 % ( w / w ), or from about B -caryophyllene in an amount ranging from about 0.5 %
2.7 % ( w / w ) to about 3 % ( w / w ). ( w / w ) to about 0.7 % ( w / w ). In some embodiments, the
In some embodiments the invention provides a cannabis cannabis oil prepared from the Cannatonic cannabis strain
oil prepared from the AC /DC cannabis strain , wherein the further contains a - humulene in an amount ranging from
oil contains: THC in an amount ranging from about 1 % 5 about 0.3 % ( w / w ) to about 0.4 % ( w / w ). In some embodi
( w / w ) to about 3 % ( w / w ); CBD in an amount ranging from ments, the cannabis oil prepared from the Cannatonic can
about 58 % ( w / w ) to about 66 % ( w / w ); CBG in an amount nabis strain further contains cis -nerolidol in an amount
ranging from 2 % ( w /w ) to about 4 % ( w / w ); and CBN in an ranging from about 0.1 % ( w / w ) to about 0.3 % ( w / w ). In
amount ranging from about 0.05 % ( w / w ) to about 0.15 % some embodiments, the cannabis oil prepared from the
( w / w ). In some embodiments, the cannabis oil prepared 10 Cannatonic cannabis strain further contains guaiol in an
from the AC/ DC cannabis strain further contains CBDA in amount ranging from about 0.2 % ( w / w ) to about 0.4 %
an amount ranging from about 0.2 % ( w / w ) to about 0.9 % ( w / w ). In some embodiments, the cannabis oil prepared
( w / w ). from the Cannatonic cannabis strain further contains
In some embodiments, the cannabis oil prepared from the a - eudesmol in an amount ranging from about 0.1 % ( w / w ) to
AC/DC cannabis strain further contains linalool in an 15 about 0.3 % ( w / w ). In some embodiments, the cannabis oil
amount ranging from about 0.1 % ( w / w ) to about 0.3 % prepared from the Cannatonic cannabis strain further con
( w / w ). In some embodiments, the cannabis oil prepared tains B -eudesmol in an amount ranging from about 0.1 %
from the AC/DC cannabis strain further contains ( w / w ) to about 0.2 % ( w / w ). In some embodiments, the
ß -caryophyllene in an amount ranging from about 0.5 % cannabis oil prepared from the Cannatonic cannabis strain
( w / w ) to about 0.9 % ( w / w ). In some embodiments , the 20 further contains a - bisabolol in an amount ranging from
cannabis oil prepared from the AC/DC cannabis strain about 0.1 % ( w / w ) to about 0.3 % ( w / w ). In some such
further contains ß - caryophyllene oxide in an amount ranging embodiments, the cannabis oil prepared from the Canna
from about 0.01 % ( w / w ) to about 0.3 % ( w /w ). In some tonic cannabis strain contains terpenes in a total amount
embodiments , the cannabis oil prepared from the AC/DC ranging from about 0.5 % ( w / w ) to about 3.5 % ( w / w ).
cannabis strain further contains a -humulene in an amount 25 In some embodiments the invention provides a cannabis
ranging from about 0.2 % ( w / w ) to about 0.5 % ( w / w ). In oil prepared from the Blueberry cannabis strain , wherein the
some embodiments, the cannabis oil prepared from the oil contains: THC in an amount ranging from about 60 %
AC/DC cannabis strain further contains cis - nerolidol in an ( w / w ) to about 80% ( w / w ); CBD in an amount ranging from
amount ranging from about 0.1 % ( w / w ) to about 0.3 % about 0.5 % ( w / w ) to about 2.5 % ( w / w ); CBG in an amount
( w / w ). In some embodiments, the cannabis oil prepared 30 ranging from 1 % ( w / w ) to about 2 % ( w / w ); and CBN in an
from the AC /DC cannabis strain further contains guaiol in amount ranging from about 0.5 % ( w / w ) to about 1.5 %
an amount ranging from about 0.3 % ( w / w ) to about 0.7 % ( w / w ). In some embodiments, the cannabis oil prepared
( w / w ). In some embodiments, the cannabis oil prepared from the Blueberry cannabis strain further contains THCA
from the AC/DC cannabis strain further contains a - eudes in an amount ranging from about 0.1 % ( w / w ) to about 0.5 %
mol in an amount ranging from about 0.4 % ( w / w ) to about 35 ( w / w ). In some embodiments, the cannabis oil prepared
0.5 % ( w / w ). In some embodiments, the cannabis oil pre from the Blueberry cannabis strain further contains CBDA
pared from the AC/ DC cannabis strain further contains in an amount ranging from about 0.01 % ( w / w ) to about 0.3 %
B - eudesmol in an amount ranging from about 0.3 % ( w / w ) to ( w / w ). In some embodiments, the cannabis oil prepared
about 0.4 % ( w / w ). In some embodiments, the cannabis oil from the Blueberry cannabis strain further contains CBGA
prepared from the AC/DC cannabis strain further contains 40 in an amount ranging from about 0.1 % ( w / w ) to about 0.5 %
y - eudesmol in an amount ranging from about 0.1 % ( w / w ) to ( w / w ).
about 0.2 % ( w / w ). In some embodiments, the cannabis oil In some embodiments, the cannabis oil prepared from the
prepared from the AC/DC cannabis strain further contains Blueberry cannabis strain further contains linalool in an
a - bisabolol in an amount ranging from about 0.8 % ( w / w ) to amount ranging from about 0.3 % ( w / w ) to about 0.4 %
about 1.1 % ( w /w ). In some such embodiments, the cannabis 45 ( w / w ). In some embodiments, the cannabis oil prepared
oil prepared from the AC/DC cannabis strain contains from the Blueberry cannabis strain further contains a -ter
terpenes in a total amount ranging from about 4 % ( w / w ) to pineol in an amount ranging from about 0.1 % ( w / w ) to about
about 7% ( w /w ). 0.2 % ( w / w ). In some embodiments, the cannabis oil pre
In some embodiments the invention provides a cannabis pared from the Blueberry cannabis strain further contains
oil prepared from the Cannatonic cannabis strain , wherein 50 ß - caryophyllene in an amount ranging from about 0.7 %
the oil contains: THC in an amount ranging from about 2 % ( w / w ) to about 1.0 % ( w / w ). In some embodiments , the
( w / w ) to about 40% ( w / w ); CBD in an amount ranging from cannabis oil prepared from the Blueberry cannabis strain
about 30 % ( w / w ) to about 70% ( w / w ); CBG in an amount further contains ß -caryophyllene oxide in an amount ranging
ranging from 1 % ( w / w ) to about 4 % ( w / w ); and CBN in an from about 0.1 % ( w / w ) to about 0.2 % ( w / w ). In some
amount ranging from about 0.01 % ( w / w ) to about 2 % ( w / w ). 55 embodiments , the cannabis oil prepared from the Blueberry
In some embodiments, the cannabis oil prepared from the cannabis strain further contains a -humulene in an amount
Cannatonic cannabis strain further contains CBDA in an ranging from about 0.4 % ( w / w ) to about 0.6 % ( w / w ). In
amount ranging from about 0.01 % ( w / w ) to about 0.3 % some embodiments, the cannabis oil prepared from the
( w / w ). In some embodiments, the cannabis oil prepared Blueberry cannabis strain further contains valencene in an
from the Cannatonic cannabis strain further contains CBGA 60 amount ranging from about 0.1 % ( w / w ) to about 0.2 %
in an amount ranging from about 0.07 % ( w / w ) to about 0.3 % ( w / w ). In some embodiments , the cannabis oil prepared
( w /w ). from the Blueberry cannabis strain further contains cis
In some embodiments, the cannabis oil prepared from the nerolidol in an amount ranging from about 0.4 % ( w / w ) to
Cannatonic cannabis strain further contains linalool in an about 0.6 % ( w / w ). In some embodiments, the cannabis oil
amount ranging from about 0.1 % ( w / w ) to about 0.3 % 65 prepared from the Blueberry cannabis strain further contains
( w / w ). In some embodiments, the cannabis oil prepared a - eudesmol in an amount ranging from about 0.1 % ( w / w ) to
from the Cannatonic cannabis strain further contains about 0.2 % ( w / w ). In some such embodiments , the cannabis
 US 10,806,707 B2
41 42
oil prepared from the Blueberry cannabis strain contains or more added essential oils selected from a lavender
terpenes in a total amount ranging from about 3 % ( w / w ) to essential oil and a lemongrass essential oil .
about 5 % ( w / w ). In some embodiments, the cannabis oil extract includes
Experimental procedures for determining the cannabinoid one or more added essential oils selected from Sweet Orange
and terpene composition of the strains of cannabis used in 5 (Citrus sinensis spp ) , Peppermint (Mentha piperita spp ) ,
the disclosure herein can be performed using known tech Lemon (Citrus limon spp ) , Lavender ( Lavendula angustifo
niques in the art. The extract, any aliquot taken during the lia spp ) and Vanilla ( Vanilla planifolia spp ) . In some
extraction procedure , or the plant material itself can be used embodiments, the cannabis oil extract includes Vitamin E
in any of the quantitative analysis techniques used . Those and one or more essential oils selected from Sweet Orange
techniques include, but are not limited to , liquid chroma- 10 (Citrus sinensis spp ) , Peppermint (Mentha piperita spp ) ,
tography, mass spectrometry, and gas chromatography. A Lemon (Citrus limon spp ) , Lavender ( Lavendula angustifo
person of skill in the art will recognize that there are many lia spp ) and Vanilla ( Vanilla planifolia spp ) .
other techniques available to determine cannabinoid and Other essential oils that can be used in the compositions
terpene composition of the cannabis strains used herein . of the invention include, but are not limited to : Agarwood ;
B. Essential Oils and Other Additives 15 Agarwood Attar; Ahibero ; Allspice ; Almond, bitter ; Amber
In certain embodiments, one or more essential oils are Oil ; Ambrette Seed ; Amyris; Angelica Root ; Angelica Seed ;
added to the extracted cannabis oil to provide properties Aniseed ; Anise; Anise ( star); Armoise (Mugwort ); Artemisia
such as improved palatability . Essential oils can also provide vestita ; Asafoetida; Bakul; Balsam of Peru Oil ; Balsam of
antioxidant and preservative properties in the cannabis oil Peru Resin ; Balsamite ; Baobab Oil ; Basil , Sweet ct Lina
compositions . The identity and amount of the essential oil ( s ) 20 lool ; Basil , Sweet ct LinaloolOrganic ; Basil , Sweet ct
added can depend in part on factors including the strain of Methyl Chavicol Organic ; Bay ; Beeswax ; Bergamot;
cannabis that has been extracted and the desired organolep Birch ; Boldo ; Boronia ; Black Cumin ; Black Currant Bud ;
tic properties. In general, the amount of total essential oils Blue Lotus Attar; Broom ; Buchu ; Bupleurum ( Bupleurum
added to a cannabis extract will range from about 0.01 % fruticosum ); Buddha wood ; Butter; Cabreuva ; Cade ; Caju
( w / w ) to about 10% ( w / w ) or more . The total amount of 25 put; Calamus; Calendula ; Camomile (or Chamomile ); Cam
essential oils added can range, for example , from about phor; Cananga ; Cangerana; Cape Chamomile (Ericephalus
0.01 % ( w / w ) to about 0.5 % ( w / w ), or from about 0.5 % punctulatus) S. Africa, Wild Harvest; Cape May ; Caraway ;
( w / w ) to about 1 % ( w / w ), or from about 1 % ( w / w ) to about Caraway ; Cardamom ; Carnation ; Carrot Seed ; Cascarilla ;
2 % ( w / w ), or from about 2 % ( w / w ) to about 3 % ( w / w ), or Cassia ; Cassie ; Catnip ; Cedar (Cedrus ) India ; Cedarwood ;
from about 3 % ( w / w ) to about 4 % ( w / w ), or from about 4 % 30 Cedarwood , Atlas Organic; Cedarwood, Himalayan ;
( w / w ) to about 5 % ( w / w ), or from about 5 % ( w / w ) to about Cedarwood , Texas; Cedarwood, Virginia ; Celery leaf, Cel
6 % ( w / w ), or from about 6 % ( w / w ) to about 7 % ( w / w ), or ery Seed ; Chamomile , Blue ; Chamomile; Chamomile ,
from about 7 % ( w / w ) to about 8 % ( w / w ), or from about 8 % Roman ( Anthemis nobilis ); Champa Attar (Michelia cham
( w /w ) to about 9 % ( w / w ), or from about 9 % ( w / w ) to about paca ) India ; Champaca; Chaste tree ; Cilantro ; Cinnamon ;
10 % ( w / w ). In some embodiments, the amount of total 35 Cinnamon Bark; Cistus; Cistus (Cistus ladaniferus) Cor
essential oils added is about 0.05 % ( w / w ). In some embodi sica ; Citronella ; Clary Sage Absolute; Clary Sage, Bulgaria ;
ments , the total amount of essential oils added is about 1.7 % Clary Sage , Russia; Clary Sage , USA ; Clementine; Clove ;
( w / w ). In some embodiments, the total amount of essential Clove Bud ; Cacao ; Coconut Pulp ; Coffee Bean Oil ; Cognac ,
oils added is about 2.5 % ( w / w ). The % ( w / w ) values Green ; Coleus ; Combava ( fruit or leaf); Copaiba ; Coriander ;
indicated are based on the amount of essential oil added to 40 Coriander Seed ; Cucumber Hydrosol; Cumin ; Cumin Seed ;
the amount of total cannabis extract ( including vitamin E or Cypress Leaf, Cypress, Blue ; Davana ; Dill ; Elemi ; Euca
additives other than the essential oil , if applicable ) . lyptus, Blue Gum ; Eucalyptus, Blue Mallee; Eucalyptus,
In some embodiments, the cannabis oil extract includes Lemon ; Fennel ( Foeniculum vulgare) Bulgaria ; Fennel,
one or more added essential oils selected from bergamot Sweet ; Fenugreek ; Fern ( sweet) ; Fleabane ; Fir Needle ; Fir,
essential oil , blood orange essential oil , neroli essential oil , 45 Balsam ; Fir, Douglas ; Fir, Silver ; Fragonia ; Frankincense ,
peppermint essential oil , and spearmint essential oil . In some India ; Frankincense, Somalia ; Frankincense Frereana;
embodiments , the cannabis oil extract includes Vitamin E Frankincense , Oman ; Frankincense, Oman ; Frankincense,
and one or more essential oils selected from bergamot Somalia ; Galangal; Galbanum ; Geranium ; Geranium ,
essential oil , blood orange essential oil , neroli essential oil , Egypt; Geranium , Rose ; Geranium , South Africa; Ghandi
peppermint essential oil , and spearmint essential oil . 50 root; Ginger; Ginger Lily ; Ginger, Fresh ; Gingergrass (Cym
In some embodiments, the cannabis oil extract includes bopogon martinii) ; Goldenrod ; Grapefruit, Pink ; Grapefruit,
one or more added essential oils selected from bergamot Ruby Red ; Grapefruit, White ; Hay ; Helichrysum , Albania ;
essential oil , blood orange essential oil , and neroli essential Helichrysum , Croatia ; Hina Attar, India; Hop ; Hyssop
oil . In some embodiments, the cannabis oil extract includes Decumbens ; Hyssop ; Immortelle ; Jasmine Absolute , Egypt ;
Vitamin E and one or more added essential oils selected 55 Jasmine Absolute , India ; Jasmine Concrete ; Jasmine ; Jas
from bergamot essential oil , blood orange essential oil , and mine Sambac ; Jatamansi, (Nardostachs jatamansi); Juniper;
neroli essential oil . Juniper Berry ( Juniperus communis ); Juniper Leaf/ Berry ;
In some embodiments , the cannabis oil extract includes Kaffir Lime ; Kava Kava; Labdanum ; Larch needle; Laurel
one or more added essential oils selected from peppermint ( Laurus nobilis ) Corsica ; Laurel Leaf, Lavandin , Grosso ;
essential oil and spearmint essential oil . In some embodi- 60 Lavender — High Elevation ; Lavender — Wild; Lavender
ments, the cannabis oil extract includes Vitamin E and one Absolute ; Lavender Hydrosol; Lavender, Bulgaria ; Laven
or more added essential oils selected from peppermint der, France; Lavender, Maillette ; Leleshwa; Lemon ; Lemon
essential oil and spearmint essential oil . Tea Tree ; Lemon verbena; Lemongrass; Lentisque ( Pistacia
In some embodiments, the cannabis oil extract includes lentiscus) Corsica; Lime ; Lime Essence Oil ; Lime , Dis
one or more added essential oils selected from a lavender 65 tilled ; Liquidambar ( Styrax ); Longoza; Lotus Absolute ,
essential oil and lemongrass essential oil . In some embodi Pink ; Lotus Absolute, White; Lovage leaf, Lovage root ;
ments, the cannabis oil extract includes Vitamin E and one Magnolia flower; Mandarin ; Mandarin , Green ; Mandarin ,
 US 10,806,707 B2
43 44
Red ; Mandarin , Yellow ; Mango ginger; Marjoram ; Marula porrum , Allium sativum , Allium schoenoprasum , Allium
oil ; Melissa ; Mint; Mint, Himalayan (Mentha arvensis ); tuberosum , Allspice , Almond , Alnus glutinosa , Alnus rubra,
Mitti Attar; Motia Attar (Jasmine sambac) India ; Mugwort; Aloe ferox, Aloeroot, Aloes , Aloe Vera , Aloysia triphylla ,
Mustard ; Myrrh ; Myrtle , Green ; Myrtle (Myrtus Commu Alpine Strawberry, Alpinia Officinarum , Althaea, Althaea
nis ); Nagarmotha (Cypriol ); Neem ( Azadirachta indica ) 5 Officinalis, Aluka , Alumroot, Amara Aromatica, Amaracus,
India; Neroli; Niaouli ; Nutmeg ; Nut grass ; Oakmoss Abso Amaranth , Amaranthus Hypocondriacus, Amber Touch
lute ; Oakwood; Opopanax , Sweet Myrrh ( Commiphora and -heal, Ambroise, Ambrose, Amburana, America
guidotti); Orange, Blood ; Orange , Sweet ; Orange, Wild ; Hodoimo, American Aloe , American Angelica, American
Orange Blossom ; Orange Essence Oil ; Orange , Bitter Ash, American Aspen, American Basswood , American Bay
Green; Orange, Bitter Red ; Oregano ; Orris Butter ; Osman- 10 berry, American Bee Balm , American Beech , American
thus Absolute ; Palmarosa ; Palmarosa, Nepal ; Palmarosa , Sri Bugleweed, American Carob, American Cranesbill, Ameri
Lanka ; Palo Santo (Bursera graveolens ); Palo Santo ; can Cress , American Dill , American Dogwood , American
Patchouli; Absolute ; Patchouli, Dark ; Patchouli, Light; Ginseng , American Ground Lily, American Groundnut,
Patchouli, Sri Lanka ; Pennyroyal; Pepper, Black ; Pepper American Linden , American Mandrake, American Melissa ,
corn , Pink ; Peppermint, Chocolate ; Peppermint, France; 15 American Saffron , American Sanicle , American Sarsapa
Peppermint, India ; Peppermint, USA ; Petitgrain Absolute ; rilla, American Sloe , American Spikenard , American Upland
Petitgrain Bigarade; Petitgrain sur Fleurs ; Petitgrain , Man Cotton , American Valerian , American Winter Cress , Ameri
darin ; Pimento ; Pine ; Pinion Juniper Co -distillation , Colo can Wormroot, American Wormseed , Amla , Ammi Visnaga,
rado , Wild Harvest; Pinon Pine ( Pinus edulis) Colorado, Anacardium Occidentale, Ananas Comosus, Anchusa Offi
Wild Harvest; Pitta blend (Lavender, Rose Geranium , Ruh 20 cinalis, Andiroba, Andrographis, Andrographis paniculata ,
Khus ); Plai ; Pomegranate Seed ; Rhododendron (Rhododen Anemone, Anemone Pulsatilla, Anemopsis californica,
dron anthopogon ); Rhododendron Leaf; Rosalina; Rose ; Anethum Graveolens, Angelica, Angelica Archangelica,
Rose Attar ; Rose de Mai Absolute ; Rose de Mai Concrete ; Angelica Sinensis, Angelica Tree , Angostura, Angostura
Rose de Mai Organic Extract; Rose geranium ; Rose Hip trifoliata, Anise, Aniseed , Aniseed Stars , Anise Fern , Anise
Seed ; Rose Otto , Bulgaria ; Rose Otto , Turkey; Rose Otto , 25 Hyssop , Anise Plant, Annatto , Annona muricata , Annona
White Organic; Rose vetiver ; Rosemary Antioxidant; reticulata , Annual Marjoram , Anthemis Nobilis, Anthoxan
Rosemary ct Cineole ; Rosemary ct Verbenone ; Rosewood ; thum nitens, Anthriscus cerefolium , Antilles Cherry, Apios
Rue; Ruh Khus ( Vetiveria zizaniodes ); Saffron Attar , India ; americana , Apium Graveolens, Apple, Apple Mint, Apple
Sage ; Samphire (Cristhmum maritimum ) Corsica ; Sandal of-Peru , Apricot Vine, Apsidium , Aralia Racemosa , Arbe a
wood ; Sandalwood , New Caledonia ; Sandalwood , Austra- 30 suif, Arberry, Arboloco , Arbor Vitae, Arbutus, Arbutus men
lian — Premium ; Sandalwood ( Santalum spicatum ), Austra ziesii, Arbutus Uva Ursi, Archangel,Archangelica, Archan
lia ; Sandalwood Oil , Royal Hawaiian ( Santalum gelica officinalis, Arctium Lappa, Arctostaphylos Uva Ursi,
paniculatum ); Sandalwood , Royal Hawaiian ; Sassafras; Ardraka, Argan , Argania, Argania spinosa, Argemone Mexi
Savitri Rose Perfume; Sea Buckthorn ; Seaweed ; Sierra cana , Argentine, Aristolochia serpentaria , Aristotelia chil
Juniper ( Juniperus occidentalis ); Spearmint; Spearmint 35 ensis, Aritha , Arjaka, Arjuna, Armoracia Rusticana , Arm
(Mentha Spicata ) Israel; Spikenard ; Spikenard , Green ; strong, Arnica , Arnica Flowers, Arnica Montana , Amica
Spruce , Black ; Spruce ( Picea mariana ) Canada ; St. John's Root , Aromatic Sumac , Aromatic Wintergreen , Arrowroot,
Wort 2 ; St. John's Wort ( Hypericum perforatum ) Bulgaria ; Artemisia , Artemisia Abrotanum , Artemisia Absinthium ,
Tagetes; Tamanu ( Foraha ) Oil ; Tangelo ; Tangerine ; Tanger Artemisia capillaris, Artemisia Dracunculus, Artemisia Tri
ine Murcott; Tansy ; Tansy, Blue ; Tarragon ; Tea Tree; Tea 40 dentata, Artemisia Vulgaris, Artocarpus altilis, Artocarpus
Tree ( Leptospermum citratum ), Lemon Scented ; Tea Tree heterophyllus, Arugula, Asafoetida, Asclepias Tuberosa ,
(Melaleuca alternifolia ) South Africa; Thuja ; Thyme; Ascophyllum nodosum , Ash , Ashwaganda, Asian Ginseng,
Thyme ct Linalool ; Tobacco ; Tonka Bean ; Tuberose; Tulsi, Aspalathus Linearis, Asparagus Cochinchinensis, Aspara
Holy Basic Oil ( Ocimum sanctum ); Turmeric ; Vanilla; gus racemosus, Asparagus Root , Asperula Odorata , Aspilia ,
Vanilla Bourbon ; Verbena; Vetiver Double Distilled ; 45 Aspilia Mossambicensis, Ass - ear, Asthma Plant, Asthma
Vetiver, El Salvador ; Vetiver, Haiti ; Vetiver, Sri Lanka ; Weed , Astragalus, Astragalus Membranaceus, Atropa bel
Violet Leaf; White Fir (Abies concolor) ; White Lotus Attar, ladonna, Auld Wife's Huid , Autumn Crocus, Avena Sativa,
White Sage ( Salvia apiana ); Wild Carrot, Corsica ; Winter Avens, Averrhoa carambola, Avocado, Ayak Chichira, Ayuk
green ; Wintergreen; Yarrow ; Yarrow , Blue ; Ylang Ylang; Willku, Azadirachta Indica, Azafran, Babchi Seeds , Bacc ,
Yuzu ; and combinations thereof. 50 Bachelor's -button , Bacopa Monniera, Bahama Berry, Baical
The compositions of the invention can also include one or Skullcap, Bai Guo, Bai Mu Erh , Ba Ji Tian , Baldina ,
more herbal extracts of Abas, Abele, Abies balsamea , Balinghoy, Ballota nigra, Balm Mint, Balm of Gilead ,
Absinthe, Absinthium , Acacia , Acacia spp . , Acai Berries, Balmony, Balsam Copaiba , Balsam Fir, Balsam of Gilead ,
Acerola , Achillea Millefolium , Achiote , Aconite, Aconitum Balsam of Peru , Balsam Tree, Bank Cress, Banisteriopsis
Napellus , Acorus, Acorus calamus, Acorus gramineus, 55 caapi vine , Baobab , Baptisia , Baptisia Tinctoria, Barbados
Adansonia digitata , Adder's Mouth , Adderwort, Adiantum Aloe, Barbados Cherry, Barbarea verna , Barberry, Barbary
capillus -veneris, Aesculus Hippocastanum , Aframomum Fig , Bardana, Barley, Barosma Betulina, Barren Strawberry,
melegueta , African Geranium , African Ginger, Agastache Barun, Basil , Basil Thyme, Basin Sagebrush , Basketbush ,
foeniculum , Agave , Agnus Castus , Agrimonia Eupatoria , Basswood , Bastard Cardamom , Bastard Saffron , Bast Tree,
Agrimony, Agropyron Repens, Ague Grass , Ague Root, 60 Bauple Nut , Bayawas , Bayberry, Bayberry Bush , Bayberry
Ague Tree, Agueweed, Ajamoda, Ajave Seeds, Ajenjo, Wax Tree , Bay Laurel, Beaked Parsley, Bean of India, Bean
Ajowan , Ajuga Reptans, Ajvain , Ajwan, Ajwain , Akebia , Trefoil, Bearberry, Bearbind , Beard Lichen , Bear's Foot ,
Akebia quinata, Alaskan Ginseng , Alchemilla Vulgaris , Bear's - grape, Bear’s -paw , Bear's Weed , Beaumont Root ,
Alchomea Species , Alder, Alder Buckthorn , Alder Dog Beauty Leaf, Bee Balm , Bee Bread , Beech , Beechdrops,
wood, Alecost , Alehoof, Aletris, Aletris Farinosa, Alexan- 65 Beech Wheat, Bee Plant, Bee Sage , Bee's Nest , Beggar's
drian Laurel, Alexandrian Senna , Alfalfa , Algarroba, Buttons, Belladonna, Belle Isle Cress , Bellyache Root ,
Alkanet, Allheal , Alligator Pepper, Allium cepa , Allium Benjamin Bush , Benzoin Gum , Benzoin Tree, Berberidis ,
 US 10,806,707 B2
45 46
Berberis Aquifolium , Berberis Vulgaris, Berberry, Bergamot Castor Oil Plant, Catalonian Jasmine , Catchweed , Catha,
Mint, Bergamot Orange, Bertholletia Excelsa , Betel , Beth Catha edulis, Catmint, Catnep , Catnip , Catrup , Cat's Claw ,
root , Betony, Betula alba, Betula pendula , Bhang, Bian Xu , Cat’s - foot, Cat’s -play, Catswort, Cat Thyme, Catuaba, Cau
Bible Hyssop, Bible - leaf, Big Sagebrush , Bilberry, Billy lophyllum Thalictroides, Cayenne, Ceanothus Americanus,
goat Clover, Biltmore Ash , Bindweed , Bird's - foot, Bird's 5 Cedar Nut, Celandine , Celery , Centaurea Cyanus, Centau
Nest , Birthroot, Birthwort, Biscuits , Bishop's Weed, Bistort, rium Erythraea, Centaury, Centella Asiatica , Century Plant,
Bitter Aloe, Bitter Ash , Bitter Bark , Bitter Dock , Bitter Leaf, Cephaelis Ipecacuanha, Cerasee, Ceratonia Siliqua , Cereso,
Bitter Melon , Bitter Nightshade, Bitter Orange , Bitter Cetraria Islandica , Chaga Mushroom , Chai Hu , Chamael
Orange Peel , Bitter Quassia , Bittersweet, Bitter Trefoil, irium Luteum , Chamomile, Chanca piedra, Chandan , Chang
Bitter Wood, Bitterworm , Bixa orellana, Black Alder, Black 10 Pu , Chaparral, Charapilla , Chaste Berry, Chaste Tree , Chat,
Alder Tree, Blackberry, Black Cherry, Black Choke , Black Chaulmoogra, Checkerberry , Cheeseflower, Cheese Rennet,
Chokeberry, Black Cohosh , Black Cohush , Blackcurrant, Cheiranthus cheiri, Chelidonium majus, Chelone glabra,
Black Dogwood , Black Ginger, Black Haw , Black Henbane, Chenopodium Ambrosioides, Chen - Pi, Cherry Birch , Cher
Black Horehound, Black Locust , Black Mustard , Black vil , Chia , Chian, Chien, Chiang, Chickweed, Chicory, Chih
Pepper, Black Root , Black Sampson, Black Sanicle, Black 15 ma , Chi -hsueh -ts’ao , Chilean Wineberry, Chilgoza , Chili
Snakeroot, Black Stinking Horehound, Black Tany, Black Pepper, Chimaphila umbellata , China Root, Chin -ch'iao
thom , Black Thyme, Black Walnut, Black Whortleberry, mai , Chinese Angelica, Chinese Cabbage , Chinese Chives ,
Blackwort, Bladder Cherry, Bladder Fucus , Bladderpod , Chinese Foxglove , Chinese Ginseng , Chinese Gold Thread ,
Bladderwrack , Blazing Star, Blessed Herb, Blessed Thistle , Chinese Lantern, Chinese Licorice , Chinese Mustard , Chi
Blind Nettle, Bloodroot, Blood Vine, Bloodwort, Blooming 20 nese Nettle , Chinese Star Anise, Chinese Wolfberry, Chink ,
Sally, Blow Ball , Blue Balm , Blueberry, Bluebottle, Blue Chionanthus Virginicus, Chirayata, Chiretta, Chittembark ,
Cohosh , Blue Curls, Blue Dandelion, Blue Flag , Blue Giant Chives , Chocolate, Chocolate Root , Chocolate Vine, Choke
Hyssop , Blue Ginseng, Blue Gum , Blue Gum Tree, Blue Cherry, Chondrus Crispus, Christmas Tree , Chrysanthe
Iris, Blue Mountain Tea , Blue Pimpemel, Blue Rocket , mum , Chrysanthemum Balsamita, Chrysanthemum Ciner
Blue - Sailors , Blue Skullcap, Blue Violet, Blunt - leaved 25 ariifolium , Chrysanthemum Morifolium , Chuan Xin Liang,
Dock , Bodhi Tree, Bofareira, Bogbean, Bo He , Bola , Chuchupate, Church Steeples, Cicely, Cichorium Intybus,
Boldina , Boldo , Boldoa , Boldu, Boldus, Boneset , Bookoo , Cilantro , Cimicifuga , Cimicifuga Racemosa , Cinchona, Cin
Borage , Borago Officinalis, Boswellia carteri, Bo - Tree, chona Bark , Cinchona spp , Cingulum Sancti Johannis, Cin
Bottlebrush , Bouncing Bet, Bourtree , Bowman's Root , Box nabar Root , Cinnamomum camphora, Cinnamomum zey
berry, Boxwood , Brahmi, Bramble, Brandy Mint, Brassica 30 lanicum , Cinnamon , Cinnamonwood, Cinquefoil, Cirsium
alba , Brassica juncea , Brassica nigra, Brassica oleracea , Vulgare, Citroengrass, Citrus aurantium , Citrus bergamia ,
Brassica rapa Pekinensis, Brazilian Ginseng, Brazil Nut, Citrus ichangensis x Citrus reticulata var. austera, Citrus
Breadfruit, Bread Wheat, Bride's Button , Bridewort, limon , Citrus retic uta , Citrus thyme, City Avens, Clary,
Brigham Tea, Brindall Berry, Brindle Berry, Brinton Root , Clary Sage , Clear Eye , Cleavers, Clematis Stem , Clove ,
British Myrrh , Broad - leaved Dock , Bromelain , Brook Bean , 35 Clove Garlic, Clover, Clover Broom , Clove Root , Clown's
Brooklime , Broom , Broom Flowers , Broom Tops, Broom Woundwort, Clubfoot Moss , Club Moss , Cnicus benedictus,
Tea - Tree, Brown Mustard, Brownwort, Bruisewort, Bryonia Coca , Coca Shrub , Cocashweed , Cochlearia officinalis,
Alba , Bryony , Buchu, Buckbean, Buckeye, Buckler - leaved Cocklebur, Cockle Buttons , Cocoa , Codonopsis, Codonop
Sorrel, Buckthorn , Buckwheat, Bucku , Buddha Fruit, Buf sis pilosula , Coffea arabica , Coffee, Coffeeweed , Coicis ,
falo Herb , Bugbane, Bugbane Squawroot, Bugle , Bugle- 40 Coix , Coix Lachryma - jobi, Cola nitida, Colchicum , Colchi
weed , Bugloss , Bu Gu Zhi , Bugwort, Bull Flower, Bullock's cum autumnale, Coleus, Coleus forkolil, Coleus Forskohlii,
Heart, Bull’s Heart, Bunny's Ears, Bupleurum , Bupleurum Colewort, Colicroot , Colla , Collinsonia canadensis, Colo
Chinense , Bur, Burage, Burdock , Burdock Burrs, Burren rado Cough Root , Coltsfoot, Colt’s -tail , Comfrey, Commi
Myrtle, Burr Marigoldt, Burrs, Burr Seed , Bush Nut, Butch phora molmol, Commiphora mukul, Commiphora opobal
er's Broom , Butterbur, Butterfly Weed , Butternut, Butter 45 samum , Common Alder, Common Alkanet, Common Anise ,
Winter, Butterwort, Butterweed , Buttons, Caban Cherry, Common Amica, Common Ash , Common Balm , Common
Cabbage , Cabbage Palm , Cabbage Rose , Cacao , Cacari, Barberry , Common Basil , Common Blue Violet, Common
Cajeput Tree, Cajueiro , Calabar Bean , Calamint, Calamintha Broom , Common Buckthorn , Common Buckwheat, Com
Nepeta , Calamus, Calendula, Calendula Officinalis, Cali mon Bugle , Common Burnet, Common Caraway, Common
fornia Poppy, Calluna Vulgaris, Calophyllum inophyllum , 50 Centaury , Common Chamomile, Common Club Moss ,
Caltrop , Calumba, Cambodian Mint, Camel Grass , Cammel Common Cotton , Common Dock , Common Dill , Common
lia sinensis, Camocamo , Camphor, Camphor Tree, Camp Fennel, Common Fenugrec, Common Flax , Common Fox
totheca Acuminata , Camu Camu, Canabis, Canada Balsam , glove , Common Hazel , Common Holly, Common Hop ,
Canada Root , Canada Tea , Canadian Fleabane , Canaigre , Common Horehound, Common Hyssop , Common Jasmine ,
Cananga odorata , Cancerosa , Caner Root , Cancer Tree, 55 Common Juniper, Common Lavender, Common Lime ,
Candle Berry , Cane Ash , Canistel Fruit, Cankerwort, Can Common Madder, Common Mallow, Common Marjoram ,
nabis Sativa, Cape Aloe, Cape Gooseberry, Caperberry, Common Nettle, Common Oats , Common Onion , Common
Caperbush , Capers , Capon's Tail, Capparis spinosa, Cap Parsley, Common Periwinkle , Common Privet, Common
saicin, Capsella Bursa -Pastoris, Capsicum , Capsicum Rue, Common Sage , Common Sagebrush , Common Sea
Annuum , Capsicum chinense , Carambola, Carapa guianen- 60 Buckthom , Common Stinging Nettle , Common Strawberry,
sis, Caraway, Caraway Seed, Cardamom , Cardamom Seeds , Common Sundew , Common Thistle, Common Thyme,
Cardamon , Carduus Marianus, Carica Papaya , Carob, Common Wheat, Common White Jasmine, Common Wil
Carolina Jasmine , Carom , Carony Bark, Carpenter's -herb , low, Common Wormwood, Compass Plant, Compass Weed,
Carpenter's - square , Carpenter's Weed , Carrageen , Carrot, Compositae, Conium Maculatum , Consormol, Consump
Carthamus tinctorius, Carum Carvi, Cascara , Cascara 65 tive's Weed, Convallaria majalis, Convolvulus sepium , Cool
Buckthorn , Cascara Sagrada, Caseweed , Cashew Nut Shells , Tankard, Copaiba , Copal , Copaifera Species , Coptidis, Cop
Cassava , Cassia Senna, Castanea Sativa , Castor Bean Plant, tis, Coptis chinensis, Coptis Rhizome, Cordyceps,
 US 10,806,707 B2
47 48
Cordyceps sinensis, Coriander, Coriandrum sativum , Corn , Evening Star, Evergreen , Eye Balm , Eyebright, Eyeroot,
Comelian Tree, Cornflower, Cornish Lovage , Corn Mint, Fagopyrum Esculentum , Fagus Grandifalia, Fah Tolai, Fairy
Corn Poppy, Corn Rose , Comsilk , Cornus Florida, Corsican Cup , Fairy's Glove , False Acacia , False Box , False Chamo
Mint, Corsican Pepper, Corydalis, Corydalis Rhizome, mile , False Jasmine , False Saffron , False Unicorn , False
Corydalis Yanhusuo, Corylus avellana, Costmary, Cotton , 5 Valerian , False White Cedar, Featherfew , Featherfoil,
Cotton Thistle , Couch Grass, Coughroot, Coughweed , Feather Geranium , Febrifuge Plant, Felon Herb , Felonwort,
Coughwort, Countryman's - treacle, Cowbloom , Cow Cher Female Fern, Fennel, Fenugreek, Ferula Asafoetida , Fetid
vil , Cow Clover, Cow Cress , Cowgrass, Cowplant, Cowslip , Horehound , Fever Bush , Feverfew , Fever Grass, Fever Tree ,
Crampbark, Crampweed, Cranberry, Cranberry Bush , Cran Feverwort, Fiber, Ficus religiosa , Field Balm , Field Pansy,
berry Tree , Cranesbill, Crataegus Monogyna , Crataegus 10 Field Poppy, Field Pumpkin , Field Sorrel, Figwort, Filipen
Oxyacantha, Crataeva, Crataeva nurvula , Cream Of Tartar dula Ulmaria , Fir, Fir Balsam , Fireweed , Fir Pine , Fish
Tree, Creathnach , Creeping Charlie, Creeping Thyme, Creo fuddle, Five - fingers, Five - leaf, Flag Lily, Flagroot , Flanders
sote Bush , Crocus sativus, Crosswort, Croton Lechleri , Poppy, Flannelleaf, Flat - leaved Parsley, Flax , Flax Seed ,
Crowberry, Crow Corn , Cuban Oregano , Cubeb Pepper, Flax Weed, Fleabane, Flea Seed , Flesh and Blood , Fleur
Cuckoo's Cap, Cucurbita Pepo , Culantrillo , Culver's 15 de - lis , Flinders Rose , Florentine Iris , Florida Dogwood ,
Physic, Culver's Root , Cumaru , Cumaruzeiro, Cumin , Florida Fishpoison Tree, Flower de - luce, Flowering Dog
Cuminum cyminum , Curacao Aloe, Curare, Curcuma longa , wood , Flowering Willow, Flowery Knotweed, Foeniculum
Curcuma zedoaria , Cure -all, Curled Dock , Curled Mint, Vulgare, Folk's Glove , Food Of The Gods , Forsythia, For
Curly Parsley, Curry - leaf tree, Curry Tree , Cuscus , Cuscuta sythia suspensa , Fo Ti , Foxberry, Fox Geranium , Foxglove ,
Epithymum , Cusparia Bark , Custard Apple, Cutleaf Bugle- 20 Fox Grape , Foxtail, Fragaria ananassa, Fragaria vesca ,
weed , Cutweed, Cydonia Oblonga, Cymbopogon Citratus, Fragrant Balm , Fragrant Giant Hyssop , Fragrant Sumac,
Cypripedium Pubescens, Da Huang , Dalcini, Dalmatian Iris, Frankincense, Fraxinus Americana, Fraxinus Excelsior,
Dalmation Insect Flower, Dalmation Pellitory, Dalmatian French Basil , French Lilac , French Parsley, French Rose ,
Sage , Damiana , Dandelion , Dang Gui, Danish Dill , Dan French Sorrel, French Tarragon , French Thyme, Friar's Cap ,
Shen, Daruharidra , Da suan, Datura Stramonium , Daucus 25 Fringe Tree, Fritillaria , Fritillaria Thunbergii, Frog Plant,
carota, Deadly Nightshade, Deadmen's Bells , Dead Nettle , Fucus Vesiculosus, Fu - ling, Fuller's -herb, Fumaria offici
Dead -Rat Tree, Death - flower, Deerberry, Desert Cactus , nalis, Fumitory, Gagroot , Galangal, Galega officinalis, Gali
Desert Oregano , Desert Tea, Devil's -apple, Devil's Bit , pea officinalis, Galium Aparine, Galium Odoratum , Galium
Devil's - bones , Devil's Cherries , Devil's Claw, Devil's verum , Gambooge, Gan cao , Gandana, Ganja, Gan - jiang,
Club , Devil's Dung , Devil's - eye , Devil's Guts, Devil's 30 Ganoderma Lucidum , Gao Liang , Garabato , Garcinia, Gar
Herb , Devil's Plague, Dew of the Sea , Dhup, Digitalis cinia cambogia, Garcinia gummi-gutta , Garcinia Kola , Gar
Purpurea , Di Huang, Dill , Dillisk , Dillseed, Dillweed , Dilly, den Angelica, Garden Balm , Garden Basil , Garden Burnet,
Dilsk Dilo Oil Tree, Dioscorea Villosa, Diosma Betulina, Garden Cham nile , Garden C1 vil , Garden Chicory, Gar
Dipsacus Sylvestris, Dipteryx Odorata , Divale , Djamboe, den Dill , Garden Heliotrope, Garden Hyssop , Garden Lav
Doda , Dodan, Doadni, Dodder, Dog Brier, Dog Grass, Dog 35 ender, Garden Loosestrife, Garden Marigold , Garden Mint,
Rose , Dog's Mercury, Dog Tree, Dogwood, Dong Chong Garden Myrrh, Garden or Green Purslane, Garden Patience,
Xia Cao , Dong Quai , Dovefoot, Drago , Dragon's Blood , Garden Rosemary, Garden Rue, Garden Sage , Garden
Dragon's mugwort, Dragonwort, Dropsy Plant, Drosera Thyme, Garden Violet, Garlic, Garlic Chives , Garlic Sage ,
Rotundifolia, Dryopteris filix -mas, Ducks Foot , Dulse , Gaultheria Procumbens, Ge - gen, Gelsemium Sempervirens ,
Dutch Clover, Dwale , Dwarf Juniper, Dwarf Nasturtium , 40 Genista, Genista Tinctoria, Gentian , Gentiana Lutea, Gera
Dwayberry, Dyeberry, Dyer's Broom , Dyer's Greenweed, nium Maculatum , Geranium Robertianum , Geraniums, Ger
Dyer's Madder, Dyer's -saffron, Dysphania ambrosioides, man Chamomile, Germander, German Mustard, German
Early Winter Cress , Earth -smoke, Easter Flower, Easter Rue, German Tarragon , German Thyme, German Valerian ,
Giant, Eau -de -cologne Mint, Echinacea, Echinacea Angus Geum urbanum , Ghaap, Gill Run Over, Ginger, Ginkgo,
tifolia , Egg Fruit, Egg Wrack , Egyptian Privet, Eight-homed 45 Ginkgo biloba , Ginkgo Nut , Ginny Grains, Ginny Papper,
Anise , Eight-horns, Ela , Elaci , Elder, Elder -berry, Elder Ginseng, Glechoma Hederacea, Glossy Buckthom , Glycine
flower, Elecampane, Eletteria Cardamomum , Eleuthero , max , Glycyrrhiza Glabra, Goathead, Goat’s Rue, Goatweed,
Eleutherococcus Senticosus, Elk Mint, Emetic Herb , Goat Wort, Gold Coin Grass, Golden Aspen , Goldenberry,
Enandi, Endive, English Alder, English Balm , English Cat Golden Flower of Mary, Golden Loosestrife, Golden Rag
nip , English Chamomile, English Hawthorn , English Holly, 50 wort, Golden Root , Goldenrod , Goldenseal, Golden Senecio ,
English Hop , English Mandrake, English Serpentary, Eng Gold Melissa , Goldy Star, Goosefoot, Goose Grass , Goose
lish Thyme, English Valerian, English Violet, English Wall wort, Gorikapuli, Gospel Tree, Gossypium Hirsutum , Gotu
flower, Epazote , Ephedra, Ephedra Nevadensis, Ephedra Kola , Gourmet Parsley, Goutweed , Gow Choy, Graines,
Sinica , Epifagus virginiana , Epilobium Angustifolium , Epi Grains Of Paradise , Gramineus, Grape, Grape Vine, Grass ,
medium, Epimedium grandiflorum , Equisetum Arvense, 55 Grass Burdock, Gravelroot, Graviola, Graybeard, Grease
Erigeron canadensis, Eriodictyon Califomicum , Eruca vesi wood , Great Burdock , Greater Burnet, Greater Cardamom ,
caria sativa , Erythraea Centaurium , Erythroxylum Catuaba, Great Morel, Great Nettle, Great Stinging Nettle, Great Wild
Erythroxylum Coca , Eschscholzia Californica, Espinheira Valerian , Greek Hayseed, Green Ginger, Green Ozier, Green
Santa , Estragon , Ethiopian Cumin , Eucalyptus, Eucalyptus Tea, Grifola frondosa , Grindelia, Grindelia Camporum ,
Globulus, Eugenia Carophyllata, Eupatorium , Eupatorium 60 Groats, Ground Berry, Ground Cherry, Ground Holly,
Perfoliatum , Eupatorium Purpureum , Euphorbia Hirta , Ground Ivy, Ground Juniper, Ground Lemon , Ground Lily,
Euphrasia Officinalis, European Alder, European Angelica, Groundnut, Ground Pine , Ground Raspberry, Grouse Berry,
European Ash , European Barberry, European Black Alder, Guaiac , Guaiacum , Guajacum , Guaiacum officinale,
European Buckthom , European Centaury, European Chest Gualtheria procumbens, Guarana , Guasai, Guava Tree ,
nut, European Cowslip , European Dill , European Elder, 65 Guelder Rose , Guggul, Gui, Guinea Grains, Guinea Pepper,
European Holly, European Hop , European White Water lily, Guinea Seeds , Gum Bush , Gum Guggulu , Gum Myrrh Tree,
European Willow , Euterpe Oleracea , Evening Primrose , Gumplant, Gurmabooti, Gurmar, Gymnema, Gymnema syl
 US 10,806,707 B2
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vestre , Gynostemma, Gynostemma pentaphyllum , Gypsy Cure, King's -cure - all, Kinnikinnick, Kiryat, Klamath Weed ,
weed , Gypsywort, Habanero Pepper, Hackmatack , Hai Knitback , Knitbone, Knotgrass , Knotted Kelp , Knotted
ts’ao , Hamamelis Virginiana , Handflower, Happy Tree, Marjoram , Knotted Wrack , Knotty Brake, Knotweed , Kola
Hapusha, Hardock , Hareburr, Hare's Ear Root , Harpago Nut, Korean Ginseng, Kua - lou , Kuawa, Kudzu, Kuei,
phytum procumbens, Hartshorn Plant, Harvest Lice , Hasa- 5 Kumari, Kumaru , Kuo - lao, K’u -tou , Ku Ts’ai, Lactucarium ,
bis , Hashish , Hatomugi, Haw , Hawaii Nut, Hawkweed, Lactuca Virosa, Ladder - to -heaven , Ladies ’- delight, Ladies '
Hawthorn , Haymaids , Hazelnut Tree , Heal - All , Heart Of Seal , Lady Bleeding , Lady's Bedstraw , Lady's Slipper,
The Earth , Heartsease , Heather, Hebanthe Paniculata , Lady's Mantle, Lady’s -washbowl, Lai - ei -ts’ao , Lamb Mint,
Hedge Bind Weed, Hedge Fumitory, Hedge Maid , Hedion Lamium Album , Lammint, Lang - tu, Langue de Boeuf, Lapa
dilla , Helianthus Annuus , Heliotrope, Hellweed , Helmet 10 cho , Lappa, Lappa Minor, Large Fennel, Large- leaved Ger
Flower, Helonias Root , Hemlock , Hemp, Henbane, Henna, mander, Larrea Tridentata , Latherwort, Laurus Nobilis, Lav
Herb Bennet, Herb -of-Grace, Herb of St. Barbara, Herb of ender, Lavender Giant Hyssop , Lavandula officinalis, Lawn
The Angels, Herb -of -the - cross, Herb Robert, Hercules Chamomile, Lawsonia inermis Leafcup , Lebanese
Woundwort, He Shou Wu, Hibiscus, Hibiscus Sabdariffa, Oregano, Leeks , Lemon, Lemon Balm , Lemon Thyme,
Hieracium Pilosella , Hierochloe odorata, High Angelica , 15 Lemon Verbena, Lemongrass, Lentinus Edodes, Leonurus
High Mallow , Hill Berry, Hina , Hind Heal , Hineheel, Hing , Cardiaca , Leopard's Bane, Lepidium meyenii , Leptandra,
Hini, Hippophae rhamnoides, Hip Rose , Hoarhound , Hock Leptandra Virginica , Leptospermum scoparium , Lesser
heal , Hodoimo , Hoelen, Hog Apple , Hog Bean , Hog Cran Indian Cress , Lesser Periwinkle , Le -ts’ao , Lettuce Opium ,
berry, Hogweed , Holigold , Holly, Holy Basil, Holy Ghost Levisticum Officinale, Lian Qiao , Licorice , Licorice Mint,
Plant, Holy Herb, Holy Grass, Holy Thistle , Honey Plant, 20 Licorice Root , Life Root , Lignum Vitae, Ligusticum Porteri ,
Honeysuckle, Hoodia , Hoodia pilifera , Hood Weed , Hood Ligustrum Vulgare, Lily Convalle, Lily of the Valley,
wort, Hook -heal, Hopniss , Hops , Hops Vine, Horehound, Limaosinho , Limeblossom , Lime Flowers , Lime Mint, Lime
Horny Goatweed, Horse Balm , Horse Chestnut, Horsefly Tree , Linden , Linden Flower, Lindera benzoin , Ling Chi,
Weed, Horseheal, Horse Mint, Horseradish , Horse Savin , Ling -t’ung, Ling Zhi, Link , Linseed, Lint Bells , Linum
Horsetail, Horse Thistle , Horseweed, Ho She Wu, Ho Shou 25 Usitatissimum , Lion's Ear, Lion's Foot , Lion's Tail, Lion's
Wu, Hot Mint, Hsia -ku - ts’ao , Hsiao -hui -hsiang, Hsieh -tzu Tart, Lion's Tooth , Lippia graveolens, Lipstick Tree ,
ts’ao , Hua - Hsian, Huang Qi , Huang Quin, Huarango , Hu Liquorice, Live - Forever, Live - Long , Liver Lily, Liverwort,
chin -ts’ao, Huckleberry, Hu - lu -ba , Hu - lu -pa , Humulus Lizard's Tail, Lobelia , Lobelia Inflata , Longevity Herb ,
Lupulus, Hungarian Chamomile, Huo Ma Ren , Hurrburr, Lonicera Caprifolium , Lonicera Japonica, Lonicera Spp ,
Hurtleberry , Hurtsickle , Husk Cherry, Hydnocarpus, Hyd- 30 Loodroot, Loosestrife, Lophophora williamsii, Lotus, Lotus
nocarpus kurzii , Hydrangea, Hydrangea Arborescens, Comiculatus, Lousewort, Lovage , Love Apples, Love in
Hydrastis Canadensis, Hyoscyamus Niger, Hypericum , Winter, Love - lies -bleeding, Love Persley, Love Vine,
Hypericum Perforatum , Hyssop , Hyssopus Officinalis, Ice Luceme , Lu Hui , Lungwort, Luole , Lychee , Litchi chinensis,
land Lichen , Iceland Moss , I - chi - kao , Ignatia Amara , Igna Lycium , Lycium Chinense, Lycium Fruit, Lycopodium , Lyco
tius Bean, Ilang - Ilang, Ilex Aquifolium , Ilex Paraguariensis, 35 podium clavatum , Lycopus americanus, Lysimachia chris
Illicium verum , Ill - scented Sumac , Imburana De Cheiro , tinae , Lysimachia vulgaris, Lythrum salicaria, Ma Bian Cao ,
Incensier, Indian Apple, Indian Arrowroot, Indian Balm , Macadamia Nut, Macadamia spp . , Maca , Maca - Maca ,
Indian Balmony, Indian Bedellium , Indian Borage , Indian Mace , Macochihua, Madagascar Periwinkle, Mad Apple,
Bread, Indian Chickweed , Indian Corn , Indian Dye , Indian Madder, Madder Root , Madderwort, Mad Dog , Madweed ,
Elm , Indian Gentian, Indian Ginseng , Indian Gooseberry, 40 Madrone Tree , Ma huang, Maidenhair Fern , Maidenhair
Indian Lotus, Indian Mustard , Indian Nettle, Indian Nut, Tree, Maid's - hair, Maino , Maitake , Maize , Maka , Malabar
Indian Olibanum , Indian Paint, Indian Pennywort, Indian Cardamom , Malabar Plum , Malabar Tamarind, Mal- dos
Pink, Indian Plant, Indian Plume , Indian Potato , Indian Red sete -dias , Male Fern , Mallow, Malpighia species , Malus
Paint, Indian Root , Indian Sage , Indian Shamrock , Indian Communis, Malva Sylvestris, Mamey Sapote, Manac, Man
Snakeroot, Indian Tobacco , Indian Tree, Indigo Broom , 45 dioc , Mandragora, Mandragora Officinarum , Mandrake,
Inonotus obliquus, Inula Helenium , Ipecac , Ipecac Shrub , Mangosteen Oil Tree, Manihot esculenta , Manioc, Manioc
Ipio , Iporoni, Iporuru, Iris, Iris Florentina , Iris Germanica , Root , Manna Grass, Man - t’ien -hsing, Manuka, Manuka
Irish Broom , Iris pallida, Irish Moss , Iris Versicolor, Italian Myrtle, Manuka Tree , Manzanilla , Maqui, Maramar,
Bumet, Italian Cress, Italian Jasmine , Italian Lovage , Italian Maranta Arundinaceae, Maranta Starch , Marapuama,
Pimpemel, Ivory Plum , Jaborandi, Jackfruit, Jack Tree , Jak, 50 Mare’s Tail, Marigold, Maroochi Nut, Marrubium , Marru
Jacob's Chariot, Jacob's - staff, Jacón , Jamaican Dogwood , bium Vulgare , Marshmallow , Marsh Marigold , Marsh Pars
Jamaica Pepper, Jamaica Sorrel, Jambu, Jambul, Jamestown ley, Marsh Trefoil, Marsh Woundwort, Marum , Marvel,
Weed , Japanese Catnip , Japanese Grapefruit, Japanese Mary Bud , Mary Golde, Mary Gowles , Mary Jane, Mary
Horseradish , Japanese Mint, Japanese Mushroom , Japanese land Pink , Mary's Grass, Mary's Mantle , Mary's Thistle ,
Seaweed , Jasmin, Jasmine , Jasmini Flos , Jasminum spp . , 55 Master of the Woods, Masterwort, Mastic , Mate , Matricaria
Jateorhiza Palmata , Jaundice Berry , Jaundice Root , Java Chamomilla , May, Mayapple, May Blossom , Maybush ,
Pepper, Java Plum , Jersey Tea , Jerusalem Oak , Jessamine, May Lily, Maypop , Maytenus, Maytenus Species , May Tree,
Jesuit's Balsam , Jesuit Tea , Jew's -harp Plant , Jiaogulan, Meadow Clover, Meadow Eyebright, Meadow Saffron ,
Jicara , Jimsonweed , Jing Jie , Jin Qian Cao , Jin Yin Hua , Meadow Sage , Meadowsweet, Mealberry, Medicago Sativa ,
Job's Tears, Joe Pye Weed , Juglans cinerea , Juglans Nigra, 60 Mei - ts’ao , Melaleuca, Melaleuca Altemifolia , Melegueta
Juglans Regia , Johnny - jump -up , Johnswort, Joint Fir , Ju Pepper, Melilot , Melilotus Officinalis, Melissa, Melissa Offi
Hua , Juniper, Juniper Bark , Juniper Berry, Juniper Bush , cinalis, Melmot Berry, Mentha haplocalyx, Mentha piperita ,
Jupiter's Bean, Juniperus Communis , Kachur, Kalmegh, Mentha pulegium , Mentha requienii , Mentha suaveolens,
Kamoteng Kahoy, Kanma, Kan - ts'ao , Kappa, Katphala , Mentha spicata, Menthol Mint, Mentha x piperita citrata ,
Kava Kava , Kelp , Kelpware , Kemangen , Keyflower, Key of 65 Menyanthes Trifoliata , Mercurialis Perennis, Mescal ,
Heaven , Khas Khas, Khat, Khella , Kiawe, Kidney Stone Meshasringi , Mesquite, Methi, Mexican Mint, Mexican
Tree, King Of Bitters , King's - clover, King's Crown, King's Oregano, Mexican Poppy, Mexican Potato , Mexican Tea ,
 US 10,806,707 B2
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Mexican Thyme, Mexican Wild Yam , Mexico Seed , Middle Pigeon's Grass, Pignoli, Pigweed , Pikake, Pill -Bearing
Comfrey, Mignonette Tree , Mi-kan , Milfoil, Milk Ipecac , Spurge, Pilocarpus Microphyllus, Pilosella , Pimbina,
Milk Thistle , Milkwort, Millefoil, Mint, Mints , Miracle Pimenta Dioica , Pimenta Officinalis, Pimpinella Anisum ,
Herb , Miracle Tea, Mistletoe , Mitchella Repens, Mi- tieh Pine, Pine Nut , Pineapple, Pineapple Strawberry, Pineapple
hsiang, Mi -ts’ao , Moccasin Flower, Mogo , Molina , Mo Li 5 Verbena , Pinkroot, Pink Rose , Pinon Nut, Pinus spp . , Pinus
Hua , Momordica Charantia , Monarda Didyma, Monkey Strobus, Pinus Sylvestris, Pinyon Pinenut, Piper betle, Piper
Bread Tree , Monkshood, Monk's Pepper, Moonflower, cubeba , Piper Methysticum , Piper Nigrum , Pipe Tree , Pip
Moon Grass, Moose Elm , Morinda , Morinda citrifolia , sissewa , Pistachio , Pistacia vera , Piscidia piscipula , Pissa
Morinda officinalis, Mormon Tea, Moroccan Ironwood, bed , Pistachio , Plantago Major, Plantago Psyllium , Plan
Mortification Root , Morus Nigra , Mother -of- thyme, Moth- 10 tago Seed , Plantain , Plectranthus amboinicus , Pleurisy Root ,
er’s -heart, Motherwort, Moujean Tea , Mountain Amica, Plum Rose , Podophyllum Peltatum , Poet's Jasmine , Pogos
Mountain Ash , Mountain Aspen , Mountain Balm , Mountain temon cablin , Poha Berry, Poison Ash , Poison Flag , Poison
Berry, Mountain Box , Mountain Cranberry, Mountain Daisy, Parsley, Poison Tobacco , Pokeroot, Pokeweed , Polar Plant,
Mountain Grape, Mountain Holly, Mountain Mint, Moun Polygala Senega , Polygonatum Multiflorum , Polygonum
tain Savory, Mountain Strawberry, Mountain Tobacco , 15 Aviculare, Polygonum Bistorta, Polygonum Multiflorum ,
Mountain Tea, Mouse - ear, Mugwort, Muira Puama, Polygonum odoratum , Polypodium Vulgare, Polypody, Poor
Mujonso , Mulberry, Mullein , Murraya koenigii, Muscatel Man's Ginseng , Poor -man’s - treacle, Poplar, Popotillo ,
Sage , Mu - Su , Mu Tong, Mu - yao , Myrciaria dubia , Myrica, Populus alba , Populus Tremuloides, Poria , Poria cocos,
Myrica Cerifera, Myricae Cortex, Myristica Fragrans, Portulaca Oleracea , Pot , Potato Bean , Potency Wood ,
Myroxylon Balsamum , Myroxylon Pereirae, Myrrh , Myr- 20 Potentilla Anserina , Potentilla Erecta , Potentilla Reptans,
rhis odorata , Myrtle , Myrtle Pepper, Myrtus communis , Potenzholz, Pot Marigold, Pouteria sapota , Pouteria campe
Nagara , Naidi , Naked Ladies, Napa Cabbage , Nappa , Nar chiana , Prairie Smoke , Prickly Ash, Prickly Lettuce, Prickly
row Dock, Narthex, Nashia inaguensis, Nasturtium Offici Pear Cactus, Prickly Poppy, Priest’s -crown, Prim , Primrose ,
nale, Naughty Man's Cherries, Neem , Nelumbo nucifera, Primula Veris, Prince's Feather, Prince’s -pine, Privet,
Nenuphar, Nep , Nepeta Cataria , Nerveroot, Nettle, Nettle 25 Prosopis pallida syn . Prosopis limensis, Provence Rose ,
Flowers , New England Pine , New Jersey Tea , New Zealand Prunella , Prunella Vulgaris, Prunus Amygdalus, Prunus
Tea Tree, Niando , Nicotiana Rustica , Nightshade, Night Dulcis, Prunus Serotina, Prunus Spinosa, Psidium Guajava,
Willow Herb , Nine Hooks , Nine Joints , Nip , Nira , Niu Bang, Psoralea, Psoralea corylifolia, Psoralea Fruit, Psyllium , Pty
Noble Chamomile, Noble Yarrow , Nodding Wakerobin , chopetalum ovata , Pueraria Lobata , Puff Ball , Pu gong
Noni , Normandy Cress, Northern Pine , Northern Spicebush , 30 ying , Pukeweed , Pu -kung -ying, Pulmonaria Officinalis, Pul
Northern White Cedar, Norwegian Kelp , Nosebleed , Nut satilla , Pumpkin , Pumpkin Pine, Puncture Vine, Purging
meg , Nux Vomica, Nymphaea Alba , Oak, Oats , Ocimum Buckthorn , Purple Angelica , Purple Betony, Purple Clover,
basilicum , Ocimum tenuiflorum , Oenothera Biennis , Ohio Purple Coneflower, Purple Foxglove , Purple Leptandra ,
Curcuma , Oil plant, Oilnut, Oil Nut Tree, Old English Purple Loosestrife, Purple Medic, Purple Passionflower,
Lovage, Old -maid's -nightcap, Old -maid's -pink, Old Man , 35 Purple Rocket, Purplestem Angelica , Purslane, Pygeum ,
Old -man’s -beard, Old Man's Nightcap, Old Man's Pepper, Pygeum Africanum , Pyrethrum , Pyrola Umbellata , Quack
Old woman, Olea Europaea, Olibanum , Olive , Omam , Grass, Quaking Aspen , Quassia , Quassia Bark , Quassia
Omum , One -berry, Onion , Opium Poppy, Oplopanax hor Wood , Quebra Pedra , Queen Annes Lace , Queen Of The
ridus, Orange, Orange Mint, Orange Root , Ordeal Bean , Meadow , Queensland Nut, Queen's Delight, Queen’s -root,
Oregano, Oregano Brujo , Oregon Grape, Oriental Garlic , 40 Quercus alba , Queue de Lezard , Quickbeam , Quick - set,
Oriental Mustard , Oriental Poppy , Origanum majorana , Quince , Quinine Tree , Quinsy Berries, Quiverleaf, Race
Origanum syriacum , Origanum vulgare, Orpine, Orris Root , Ginger, Racoon Berry, Rainbowweed , Rashona, Raspberry,
Osha, Osterick , Oswego Tea , Our -Lady’s -bedstraw , Our Raspberry Leaf, Rat’s Tail, Rattlebush , Rattleroot, Rattle
Lady’s -tears, Oval Buchu, Owler, Oxadoddy, Ox Balm , Ox snake Root , Rattleweed, Rau Ram , Rauwolfia , Rauwolfia
Heart, Ox -tongue , Pacific Madrone, Pacific Yew , Padmaka, 45 Serpentina, Red Alder, Red Balm , Red Bearberry, Red
Paeonia Officinalis, Paico , Paigle , Palisade Pine , Palma bearded , Red Bergamot, Redberry Tea, Red Bush Tea , Red
Christi, Palmaria Palmata , Palmetto , Panax Ginseng, Panax Clover, Redcole , Red Dulse , Red Eyebright, Red Legs , Red
Quinquefolium , Panay, Pansy, Papaver orientale, Papaver Paint Root , Red Pollom , Red Poppy, Red Puccoon , Red
Rhoeas, Papaver Somniferum , Papaya , Paper Birch , Raspberry, Red Robin , Red Root , Red Root Sage , Red Rose ,
Papoose Root , Paracress, Paraguay Tea , Pareira, Parell, 50 Red Sage , Red Sorrel, Red Sunflower, Red Tea , Red Tril
Parietaria Officinalis, Pariswort, Parsley, Parsnip , Partridge lium , Red -veined Dock , Reefer, Rehmannia , Rehmannia
berry, Pasque Flower, Passiflora, Passiflora Incamata , Pas Glutinosa, Reishi , Rhamnus Cathartica, Rhamnus Frangula,
sion Flower, Passions , Passion Vine, Pastinaca sativa, Rhamnus Purshiana, Rheumatism Root , Rheumatism Weed ,
Patchouli, Patience Dock, Patience Herb, Pau d'Arco , Paull Rheum Palmatum , Rhodiola , Rhodiola sacra , Rhubarb ,
inia Cupana , Pausinystalia Yohimbe, Pauson , Peepal Tree, 55 Rhus trilobata, Ribes Nigrum , Ribwort, Richweed , Ricinus
Pei-ma, Peking Cabbage, Pelargonium , Pelargonium Communis, Rimed scutatus, Ritha, Robinia Pseudoacacia ,
sidoides , Pelargonium species, Pellitory, Pellitory Of The Rock Brake, Rock Fern , Rockweed , Roman Chamomile ,
Wall, Pembina, Pennyroyal, Peony, Pepperidge Bush , Pep Roman Cumin , Roman Fennel, Rooibos , Root Of The Holy
permint, Pepperweed, Perfume Tree, Periploca Of The Ghost, Roquette, Rosa Canina, Rosa Centifolia, Rosa Gal
Woods, Persea Americana , Persian Berries, Persicaria odo- 60 lica , Rose, Rose Apple , Roselle , Rosemary , Rosemary Plant,
rata, Personata , Peruvian Balsam , Peruvian Bark, Peruvian Rose -noble , Rose Root , Rosin Rose , Rosmarinus Officinalis,
Ginseng , Peruvian Ground Cherry, Petasites Hybridus, Rosy Periwinkle , Rou Dou Kou , Round Buchu, Round
Petokal, Petroselinum Crispum , Peumus Boldus, Peyote , leaved Dock, Round - leafed Mint, Round- leaved Sorrel,
Pfaffia paniculata , Philanthropos, Phudina , Phyllanthus, Rowan Tree, Royal Herb , Royal Jasmine , Rub Cherry, Rubia
Phyllanthus emblica, Phyllanthus niruri, Physalis Alkek- 65 tinctoria , Rubus Fructicosus, Rubus Idaeus, Rucola, Rue,
engi, Physalis Peruviana L. , Physic Root, Physostigma Rugula, Rumara, Rumex Acetosella , Rumex Crispus, Rumex
venenosum , Phytolacca Americana , Picrasma Excelsa , Hymenosepalus, Rumex Obtusifolius, Running Club Moss ,
 US 10,806,707 B2
53 54
Ruscus Aculeatus, Russian Chamomile , Russian Mustard, Stitchwort, St. John's Bread , St. John's Grass, St. John's
Rustic's Treacle , Ruta Graveolens, Sabal, Saccharum off Plant, St. John's Wort, St. Josephwort, Stonecrop, Stone
cinarum , Sacred Bark , Sacred Basil , Sacred Fig , Sacred Root , Strawberry, Stork's Bill , Strawberry Tomato , Straw
Lotus, Sacred Plant, Sacred Sage , Sacred Tree, Sacred Water berry Tree, Striped Alder, Strophanthus, Strophanthus Gra
Lotus, Safflower, Saffron , Sagackhomi, Sage , Sage - leaved 5 tus, Strychnine, Strychnine Tree, Strychnos nux - vomica,
Germander, Sage Of Bethlehem , Sake, Salad Burnet, Salad Styrax Benzoin , Succory, Sudanese Tea, Sugar Cane , Sui
Chervil, Salad Rocket, Salai Gugal, Salix Alba, Saloip , Hoi , Suma , Su Nanesi , Sundew , Sunflower, Sunkfield ,
Salvia , Salvia apiana, Salvia hispanica, Salvia Miltiorrhiza , Sunthi, Surasa , Suterberry, Swallowwort, Swamp Cedar,
Salvia Officinalis, Salvia Sclarea , Sambucus Nigra , Samp Swamp Root , Sweating Plant, Sweet Almond, Sweet Balm ,
son Root , Sandalwood, Sandberry, Sangre de Drago , Sangue 10 Sweet Basil , Sweet Bay, Sweet Birch , Sweet Bracken , Sweet
de Drago , Sanguinaria, Sanguinaria Canadensis, San Brake, Sweet Bugle , Sweet Cane, Sweet Chervil, Sweet
guisorba Minor, Sanguisorba Officinalis, Sanicle , Sanicula Chestnut, Sweet Cicely, Sweet Clover, Sweet Coltsfoot,
Europaea, Sanicula Marilandica , Santalum Album , Sapin , Sweet Cumin , Sweet Dock, Sweet Elm , Sweet Fennel,
Sapindus mukorossi, Saponaria Officinalis, Sapote, Sarapia, Sweet Fern , Sweet Flag , Sweet Flag Rhizome, Sweet Gold
Sarepta Mustard , Sarothamnus Scoparius, Sarpagandha, 15 enrod, Sweet Grass, Sweet Iris, Sweet Lavender, Sweet
Sarsaparilla, Sassafras, Sassafras Albidum , Satan's Apple, Licorice , Sweet Luceme, Sweet Marjoram , Sweet Myrrh ,
Satavar, Satinflower, Satureja hortensis, Satureja montana , Sweet Root , Sweet Rush , Sweet - scented Geranium , Sweet
Saventaro , Savory , Saw Palmetto , Saxifrax, Scabish, Scab Tea Vine, Sweet Tongue, Sweet Violet, Sweetweed , Sweet
wort, Scarlet Bergamot, Scarlet Monarda, Scarlet Sage , Wood , Sweet Woodruff, Swertia, Swertia chirayita, Swine
Scarweed , Scented Fern , Scented Sumac, Schisandra, 20 Snout, Symphytum Officinale, Symplocarpus Foetidus, Syr
Schisandra Chinensis, Schizonepeta , Schizonepeta tenuifo ian Oregano, Syzygium cumini, Syzygium jambos , Tabe
lia , Scopolia , Scopolia camiolica , Scotch Broom , Scotch Fir, buia Spp . , Tagara, Taheebo , Ta -huang, Tailed Cubebs, Tailed
Scotch Heather, Scotch Pine , Scots Pine, Scouring Rush , Pepper, Tailwort, Tall Nasturtium , Tallow Shrub, Tall Speed
Scrofula Plant, Scrophularia Nodosa , Scurfy Pea , Scurvy well , Tall Veronica, Tamanu Nut Tree, Tamarind, Tamarin
Grass , Scurvy Weed, Scutellaria Baicalensis , Scutellaria 25 dus Indica, Tamus, Tanacetum Parthenium , Tanacetum Vul
Lateriflora , Sea Buckthom , Sea Lettuce Flakes, Sea Oak , gare, Tang Kuei , Tanners Bark , Tanner's - dock , Tan Shen ,
Sea Onion , Sea Parsley, Seaweed , Seawrack , Sedum Tele Tansy, Tap Aloe , Tapioca -root, Taraxacum Officinale, Tar
phium , Self -Heal, Seneca Snakeroot, Senecio Aureus, Sen ragon , Tartar Root , Tarweed, Taxus Brevifolia , Teaberry,
eca Grass, Senega , Senega Root , Senna, Sereh , Serenoa Teasel, Tea Tree, Te Limon , Telltime, Tenuifolia , Terminalia
Repens, Serpentary, Serpyllum , Setwall, Shameface , Shan- 30 Arjuna, Tetterberry , Tetterwort, Teucrium marum , Teucrium
cha , Sha - ren , Shan - yao , Shatamull, Shatapushpa , Shatavari, scorodonia , Thali, Theobroma Cacao , Thom Apple, Thorn
Shave Grass, Shea Tree, Sheepberry, Sheep Sorrel, Sheng Poppy, Thomtree , Thorny Burr, Thoroughwort, Thousand
Di , Sheng Di Huang, Sheng Ti Huang , Shen - jiang, Shep leaf, Thousand -seal, Three - leaved Caper, Three - leaved
herd's Knot, Shepherd's Needle , Shepherd's Purse , Shi Nightshade, Throatwort, Thuja , Thuja Occidentalis, Thumb,
Chang Pu , Shield Fern, Shih - lo , Shihuahuaco , Shiitake, 35 Thunberg Fritillaria Bulb , Thyme, Thymus citriodorus,
Shiny Asparagus , Shirokikurage, Shoofly, Short Buchu, Thymus Serpyllum , Thymus Vulgaris, Tian Men Dong,
Short - leaved Buchu, Shovelweed , Shu Di Huang, Shu Ti Tibetan Rhodiola , T’ien - shih - li , Ti Huang , Ti Huang Chiu ,
Huang, Siberian Ginseng, Sicklewort, Silkweed , Silver Tilia Americana , Tilia Cordata , Tilia Europea, Tipton Weed ,
Birch, Silver Fir, Silver Leaf, Silver - leaf Poplar, Silver Mint, Toad Flax , Tobacco Wood, Tokal, Tomillo, Tom Thumb
Silver Pine , Silver Poplar, Silver Tree - ear Fungus, Silver- 40 Nasturtium , Tongue Grass, Tonka , Tonka Bean , Tonka Bean
weed, Silybum , Silymarin, Simply Jack , Sinapis alba , Skull Tree, Tonquin Bean, Toothache Plant, Toothache Tree, Tra
cap , Skunkbush , Skunkbush Sumac , Skunk Cabbage, Slip chyspermum ammi, Treacle Mustard, Tree Moss , Tree of
pery Elm , Sloe , Smallage , Smallanthus sonchifolius , Small Joy, Tree of Life, Tree's Dandruff, Trefoil, Trembling Aspen ,
Nasturtium , Smelling - stick , Smilax Uti Lis , Smooth Cicely, Trembling Poplar, Tremella fuciformis, Tribulus, Tribulus
Smooth Strophanthus, Snakebite , Snake Lily, Snake Root , 45 terrestris, Tricolor Garlic, Trifolium pratense, Trifolium
Snakeweed, Snapping Hazelnut, Snap - Wood, Snowball repens, Trigonella , Trigonella Foenum -Graecum , Trillium ,
Tree , Snowdrop Tree, Snowflake, Snowflower, Snow Fun Trillium Erectum , Triticum aestivum , Tropaeolum minus,
gus , Soap Berry, Soapnut, Soapwort, Soft Pine , Solanum Tormentil, True Angostura, True Chamomile , True Laven
Dulcamara , Soldier's Cap , Soldier's Woundwort, Solidago der, True Oregano, True Sage , True Taragon , True Unicorn
Canadensis, Solidago Virgaurea, Solis Sponsa , Solomon's 50 Root , Tse - lan , Tuber Root , Tuckahoe, Tulasi, Tulsi, Tumeric
Seal , Solsequia, Son -before - the - father, Sorbus Aucuparia , Root , Tuna Cactus , Turkey Burrseed , Turkish Oregano,
Sour Dock , Sour Grass, Soursop, Sour Weed, Southern Turmeric, Turnera Diffusa, Turtlebloom , Turtlehead , Tussi
Ginseng, Southemwood, Sowberry , Soy, Soya , Soybean , lago Farfara, Twak , Twinflower, Tzu -mo - lo , Uassi , Ulmus
Spanish Chamomile, Spanish Chestnut, Spanish Jasmine, Rubra , Uma, Umbrella Plant, Umcka, Umckaloabo, Una De
Spanish Thyme, Spearmint, Speedwell, Spiceberry, Spice- 55 Gato , Uncaria Tomentosa, Undaria pinnatifida, Upland Cot
bush , Spicewood, Spicy Wintergreen , Spigelia Marilandica , ton , Upland Cress, Upland Cranberry, Uppagi , Upside
Spike Lavender, Spiked Loosestrife, Spikenard, Spilanthes Down Tree, Urginea Maritima, Urtica Dioica , Usnea , Usnea
acmella, Spoonwood, Spoonwort, Spotted Alder, Spotted spp . , Uva Ursi, Vaccinium Macrocarpon , Vaccinium Myr
Thistle , Spring Cress, Spring Wintergreen, Square Stalk , tillus, Valerian , Valeriana Officinalis, Vandalroot, Vanilla ,
Squawbush , Squaw Root , Squaw Tea , Squaw Vine, Squill, 60 Vanilla fragrans, Vanilla Grass, Vanilla planifolia , Varuna,
Stachys Officinalis, Stachys Palustris, Stagbush, Staghom , Vegetable Antimony, Vegetable Marrow , Vegetable Sulfur,
Stanchgrass, Star Anise, Starbloom , Star Flower, Star Fruit, Vegetable Tallow , Vegetable Wax , Venus' Basin , Venus ' -hair
Star Grass, Starweed, Starwort, Stellaria , Stellaria Media , Fern , Verbascum Thapsus, Verbena , Verbena Officinalis,
Stickwort, St. Ignatius Bean , Stillingia Sylvatica, Stingnose, Vermont Valerian, Vemonia Amygdalina, Veronica Bec
Stinking Benjamin , Stinking Christopher, Stinging Nettle, 65 cabunga, Veronica Officinalis, Vervain , Vetiver, Vetiveria
Stingless Nettle, Stinking Nightshade, Stinking Roger, zizanioides , Vetivert, Vibumum Opulus, Viburnum Prunifo
Stinking Rose , Stinking Weed , Stinking Willie , Stinkweed, lium , Vietnamese Cilantro, Vietnamese Coriander, Vietnam
 US 10,806,707 B2
55 56
ese Mint, Vinca Minor, Vinca Rosea , Vine , Viola Odorata , lis) , Bacteroides lipolyticum , Bacteroides succinogenes,
Viola Tricolor, Violet -bloom , Virginia Bugleweed, Virginia Bifidobacterium ssp . (e.g. , Bifidobacterium animalis lactis,
Dogwood , Virginia Prune, Virginia Skullcap , Virginia Sna Bifidobacterium bifidum , Bifidobacterium infantis, Bifido
keroot, Virginia Water Horehound , Viscum Album , Visnaga, bacterium lactis, Bifidobacterium longum , Bifidobacterium
Vitellaria paradoxa, Vitex, Vitex Agnus - Castus, Vitis 5 animalis, Bifidobacterium breve ), Brevibacterium lipolyti
Vinifera , Vomitroot, Wachsgag1, Wakame, Wake Robin , cum , Brevibacterium stationis, Enterococcus faecium , Kur
Waldmeister, Wallflower, Walnut, Wasabi, Wasabia thia zopfii, Lactobacillus ssp . ( e.g. , Lactobacillus acidophi
japonica , Wasei, Water Bugle , Watercress, Water Dragon , lus, Lactobacillus brevis, Lactobacillus bulgaricus,
Water Flag , Water Horehound, Water Hyssop , Water Lily, Lactobacillus casei, Lactobacillus delbrueckii LE , Lactoba
Water Mint, Water Pimpernel, Water Shamrock , Water 10 cillus gasseri, Lactobacillus helveticus, Lactobacillus lacris,
Thistle , Wattle, Wax Cluster, Wax Dolls , Wax Myrtle, Way Lactobacillus paracasei , Lactobacillus plantarumtarum ,
Bennet, Weeping Ash , Weeping Forsythia , Wheatgrass, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacil
Whinberry, Whippoorwill's - shoe, Whistling Thorn , White lus salivarius, Lactobacillus sporogenes ), Myrothecium ver
Archangel, White Ash , White Birch , White Bird's - eye , rucaria , Pseudomonas calcis, Pseudomonas dentrificans,
White Bryony, White Cedar, White Ceremonial Sage , White 15 Pseudomonas fluorescens, Pseudomonas glathei, Phanero
Chamomile , White Clover, White Deadnettle , White Endive , chaete chrysosporium , Saccharomyces boulardii, Saccharo
White Flower De Luce , White Horehound , White Jelly - leaf, myces cerevisiae , Streptococcus thermophilus, Streptomyces
White Muer, White Lotus, White Mustard , White Nettle, fradiae, Streptomyces cellulosae, Streptomyces griseoflavus,
White Pine , White Poplar, White Sage , White Tansy, and combinations thereof.
Whitethom , White Tree -ear, White Turmeric , White Walnut, 20 Homeopathic remedies can also be included in cannabis
White Willow , Whitten Tree, Whorlywort, Whortleberry, oil compositions prepared according to the invention .
Whorts, Wild Allspice , Wild Angelica , Wild Black Cherry, Examples of suitable homeopathic remedies and indications
Wild Brier, Wild Bryony, Wild Carrot, Wild Celery, Wild that can be treated with homeopathic remedies include , but
Chamomile, Wild Chicory, Wild Cotton , Wild Crocus, Wild are not limited to , adrenocorticotropic hormone ( ATCH ),
Endive, Wild Fennel, Wild Geranium , Wild Hops , Wild 25 Abies Canadensis, Abies Nigra, Abrotanum , Absinthium ,
Indigo, Wild Iris , Wild Lemon, Wild Lettuce, Wild Man Acacia Arabica , Acalypha Indica , Acetaldehyde, Acetanili
drake, Wild Marjoram , Wild Oats , Wild Opium , Wild Pansy, dum , Aceticum Acidum , Acetylsalicylicum Acidum , Achy
Wild Parsnip, Wild Passionflower, Wild Pieplant, Wild Rhu ranthis Calea , Aconite , or Aconitum Nap , Aconitum Ferox,
barb , Wild Rye, Wild Snakeroot, Wild Strawberries, Wild Aconitum Lycoctonum , Aconitum Napellus, Aconitum ,
Succory, Wild Sunflower, Wild Sweetsop , Wild Tansy, Wild 30 Radix , Acorus Calamus, or Calamus, Actaea Spicata Acry
Teasel, Wild Thyme, Wild Tobacco, Wild Valerian, Wild late , Actaea Rac , or Cimicifuga, Actaea Spic , Adamas ,
Vine, Wild Yam , Willow , Willow Herb , Windflower, Wind Adelheidsquelle, Adenosinum Cyclophosphoricum , Adeps
Root , Wineberry, Winter Berry, Wint bloom, Winter Suillus , Adipose Tissue , Adonis Vemalis, Adrenal Cortex ,
Cherry, Winter Clover, Wintergreen, Winterlien , Winter Adrenal Gland, Adrenalinum , or Epinephrine, Adrenocorti
Marjoram , Winter Savory, Winter Thyme, Winterweed , 35 cotrophin , Aesculinum , Aesculus Camea , Flos , Aesculus
Witches ' -moneybags, Witchgrass , Witch Hazel, Witch's Glabra , Aesculus Hippocastanum , Aesculus Hippocastanum
Bells , Withania, Withania Somnifera , Wolfsbane, Wolfs Flos , Aethiops Antimonialis, Aethiops Mercurialis -Minera
Claw, Woman's Long Hair, Wood Betony, Woodbine , Wood lis , Aethusa Cynapium , Agaricinum , Agaricus Campanula
Boneset, Wood Ear Fungus, Woodland Germander, Wood tus, Agaricus Campestris, Agaricus Citrinus, Agaricus Eme
land Strawberry, Wood Licorice , Woodruff, Wood Sage , 40 ticus , Agaricus Muscarius, Agaricus Pantherinus, Agaricus
Wood Strawberry, Wood Turmeric, Wood Vine, Woodward , Phalloides, Agaricus Procerus, Agaricus Semiglobatus,
Woody Nightshade, Woolly Mint, Woolly Thistle, Worm Agaricus Stercorarius, Agave Americana, Agave Tequilana,
Grass, Wormseed, Wormweed , Wormwood , Woundwort, Agnus Castus, Agraphis Nutans, Agrimonia Eupatoria ,
Wu - pa -ho, Wu -wei - zi, Wycopy, Xiao -hue -xiang, Xi Shu , Xu Agrimonia Eupatoria, Flos , Agrimonia Odorata , Flos ,
Ku Cao , Yacon , Yacuma, Yang -Mei, Yape , Yarrow , Yasmin , 45 Agrostemma Githago, Ailanthus Glandulosus, Aletris
Yasti Madhu , Yawroot, Yellow Bedstraw , Yellow Cedar, Farinosa , Alfalfa, Alisma Plantago, Allium Cepa, Allium
Yellow Dock, Yellow Eye , Yellow Ginseng , Yellow Indian Sativum , Alloxanum , Alnus Glutinosa , Alnus Serrulata , Aloe
Paint, Yellow Indian Shoe , Yellow Indigo, Yellow Jasmine, Socotrina, Alstonia Constricta , Alstonia Scholaris, Althaea
Yellow Jessamine , Yellow Lark's Heels , Yellow Locust , Officinalis, Alumen, or Alum , Alumina , Alumina Silicata ,
Yellow Loosestrife, Yellow Melilot , Yellow Mustard, Yellow 50 Aluminum Metallicum , Aluminum Muriaticum , Ambra Gri
Paint Root , Yellow Poppy, Yellow Puccoon , Yellow Rocket, sea , Ambrosia Artemisiaefolia, Ammi Visnaga, Ammonia
Yellowroot, Yellow Thistle , Yellow Vine, Yerba , Yerba cum Gummi, Ammonium Aceticum , Ammonium Benzoi
Buena , Yerba Mansa , Yerba Manza, Yerba Santa , Yin -hsing, cum , Ammonium Bromatum , Ammonium Carbonicum ,
Yin Yang Huo , Ylang Ylang Tree , Yohimbe , Yohimbine , Ammonium Causticum , Ammonium Citricum , Ammonium
Yucca , Yucca spp . , Yueh -kuei, Yuma, Yu Mi Shu, Yuzu , Yuyu 55 lodatum , Ammonium Muriaticum , Ammonium Nitricum ,
Chonta, Zaatar, Zacate Limon , Zanthoxylum Americanum , Ammonium Phosphoricum , Ammonium Picricum , Ammo
Zea Mays, Zedoary, or Zingiber Officinale. nium Tartaricum , Ammonium Valerianicum , Ammonium
Probiotics can also be included in cannabis oil composi Vanadium , Amorphophallus Rivieri, Ampelopsis Quinque
tions prepared according to the invention . Examples of folia, Amygdala Amara , Amygdalae Amarae Aqua,
suitable probiotics include, but are not limited to , Acineto- 60 Amygdalae Amarae Oleum , Amygdalus Persica , Amyl
bacter calcoaceticus, Arthrobacter agilis, Arthrobacter cit Nitrosum , Anacardium Occidentale, Anacardium Orientale,
reus, Arthrobacter globiformis, Arthrobacter luteus, Arthro Anagallis Arvensis, Ananassa , Anas Barbariae Hepatis Et
bacter simplex, Azotobacter chroococcum , Azotobacter Cordis Extractum , Anatherum Muricatum , Anchusa Offici
paspali, Azospirillum brasiliense, Azospirillum lipoferum , nalis, Anemone Nemorosa, Anemopsis Califomica, Anethum
Bacillus ssp . ( e.g. , Bacillus brevis, Bacillus coagulans, 65 Graveolens, Angelica Archangelica, Angelica Atropurpurea ,
Bacillus laterosporus, Bacillus marcerans, Bacillus pumi Angelica sinensis, Radix , Angophora Lanceolata , Angustura
lus, Bacillus polymyxa, Bacillus sphaericus, Bacillus subti Vera, Anhalonium Lewinii , Anilinum , Anilinum Sulphuri
 US 10,806,707 B2
57 58
cum , Anisum , Anthemis Nobilis, Anthemis Pyrethrum , toreum , Catalpa Bignonioides , Caulophyllum Thalictroides,
Anthoxanthum Odoratum , Anthracinum ( anthrax ), Antimo Causticum , Ceanothus Americanus , Cedron, Celtis Occiden
nium Arsenicicum , Antimonium Crudum , Antimonium talis, Cenchris Contortrix , Centaurea Tagana, Centaurium
Iodatum , Antimonium Muriaticum , Antimonium Oxydatum , Umbellatum , Flos , Cephalanthus Occidentalis, Cerasus Vir
Antimonium Sulphuratum Aureum , Antimonium Tartari- 5 giniana , Ceratostigma Willmottianum , Flos , Cereus Bon
cum , Antipyrinum , Apatite, Apiolum , Apis Mellifica, Apis plandii, Cereus Serpentinus, Cerium Oxalicum , Cetraria
Venenum Purum , Apium Graveolens, Apocynum Androsae Islandica, Chamomilla , Cheiranthus Cheiri, Chelidonium
mifolium , Apocynum Cannabinum , Apomorphinum , Apo Majus, Chelidonium Majus, Radix, Chelone Glabra, Che
morphinum Muriaticum , Aqua Marina , Aquilegia Vulgaris , nopodii Glauci Aphis, Chenopodium Anthelminticum , Che
Aralia Hispida , Aralia Quinquefolia, Aralia Racemosa , Ara- 10 nopodium Vulvaria , Chimaphila Maculata, Chimaphila
nea Diadema , Arbutinum , Arbutus Andrachne, Areca Cat Umbellata , Chininum Arsenicicum , Chininum Arsenico
echu, Argemone Mexicana, Argentum Cyanatum , Argentum sum , Chininum Muriaticum , Chininum Purum , Chininum
Iodatum , Argentum Metallicum , Argentum Muriaticum , Salicylicum , Chininum Sulphuricum , Chionanthus Virgi
Argentum Nitricum , Argentum Oxydatum , Argentum Phos nica , Chloralum , Chloramphenicolum , Chlorinum , Chloro
phoricum , Aristolochia Clematitis , Aristolochia Milhomens, 15 forum , Chlorpromazinum , Cholera , Cholesterinum , Choli
Aristolochia Serpentaria , Amica Montana, Amica Montana, num , Chromicum Acidum , Chromium Kali Sulphuricum ,
Radix , Arsenicum Album , Arsenicum Bromatum , Arseni Chromium Oxydatum , Chromium Sulphuricum , Chrysan
cum Iodatum , Arsenicum Metallicum , Arsenicum Sulphu themum Leucanthemum , Chrysarobinum , Cicer Arietinum ,
ratum Flavum , Arsenicum Sulphuratum Rubrum , Artemisia Cichorium Intybus, Cichorium Intybus, Flos , Cicuta Macu
Vulgaris, Arum Dracontium , Arum Italicum , Arum Macula- 20 lata , Cicuta Virosa, Cimex Lectularius , Cimicifuga Rac
tum , Arum Triphyllum , Arundo Mauritanica, Asafoetida , emosa , Cina, Cinchona Officinalis, Cinchonium Sulphuri
Asarum Canadense, Asarum Europaeum , Asclepias Curas cum , Cineraria Maritima, Cineraria Maritima, Succus ,
savica , Asclepias Incamata, Asclepias Syriaca , Asclepias Cinnamomum , Cistus Canadensis, Citricum Acidum , Citrus
Tuberosa , Asclepias Vincetoxicum , Asclepias Vincetoxicum Decumana , Citrus Limonum , Citrus Vulgaris, Clematis
Folia , Asimina Triloba, Asparagus Officinalis, Asperula 25 Erecta, Clematis Virginiana , Clematis Vitalba, Flos , Clema
Odorata , Astacus Fluviatilis, Asterias Rubens, Astragalus tis Vitalba , Folia , Cobaltum Metallicum , Cobaltum Muriati
Menziesii, Atropinum , Atropinum Sulphuricum , Aurum Bro cum , Cobaltum Nitricum , Coccinella Septempunctata , Coc
matum , Aurum Iodatum , Aurum Met , Arum Mur, Aurum culus Indicus, Coccus Cacti , Cochlearia Armoracia ,
Muriaticum Kalinatum , Aurum Muriaticum Natronatum , Cochlearia Officinalis, Coenzyme A , Coffea Cruda , Coffea
Aurum Sulphuratum , Avena Sativa , Aviaire, Azadirachta 30 Tosta, Colchicinum , Colchicum Autumnale , Colibacillinum ,
Indica , Bacillinum of Burnet, Badiaga , Baja, Balsamum Collinsonia Canadensis, Colocynthinum , Colocynthis ,
Peru , Baptisia Tinctoria , Barosma Cren, Baryta Acetica , Colostrum , Comocladia Dentata , Conchiolinum , Condur
Baryta Carbonica, Baryta Iodata, Baryta Mur ica , BCG , ango , Coniinum , Coniinum Bror im , Conium Maculatum ,
Belladonna , Belladonna , Radix , Bellis Perennis, Benzinum , Convallaria Majalis, Convolvulus Arvensis, Copaiva Off
Benzinum Dinitricum , Benzoicum Acidum , Benzoinum , 35 cinalis, Corallium Rubrum , Corallorhiza Odontorhiza, Cori
Berberinum , Berberis Aquifolium , Berberis Vulgaris, Ber aria Ruscifolia, Cornus Altemifolia , Cornus Circinata ,
beris Vulgaris, Fructus, Beryllium Metallicum , Beta Vul Comus Florida , Cortisone Aceticum , Corydalis Canadensis,
garis, Betainum Muriaticum , Betula Pendula , Cortex , Cotyledon Umbilicus, Coumarinum , Crataegus Oxyacan
Betula Pendula, Folia , Bismuthum Metallicum , Bismuthum tha, Cresolum , Crocus Sativus, Crotalus Cascavella, Crota
Oxydatum , Bismuthum Subnitricum , Bixa Orellana, Blatta 40 lus Horridus, Croton Tiglium , Crotonchloralum , Cubeba
Americana, Blatta Orientalis, Boldo , Boletus Luridus, Bole Officinalis, Cucurbita Citrullus, Cucurbita Pepo . Flos ,
tus Satanas, Bombyx Processionea , Borago Officinalis, Cucurbita Pepo, Semen, Culex Musca, Cuphea Petiolata ,
Borax, Boricum Acidum , Botulinum , Bovista , Brassica Cupressus Australis, Cupressus Lawsoniana , Cuprum Ace
Napus, Bromelain , Bromium , Bromus Ramosus , Flos , ticum , Cuprum Ammonio -Sulphuricum , Cuprum Arsenico
Brucinum , Bryonia Alba , Bufo Rana , Bunias Orientalis, 45 sum , Cuprum Carbonicum , Cuprum Metallicum , Cuprum
Buthus Australis, Butyricum Acidum , Buxus Sempervirens, Muriaticum , Cuprum Nitricum , Cuprum Oxydatum Nigrum ,
Cacao , Cactus Grandiflorus, Cadmium Bromatum , Cad Cuprum Sulphuricum , Curare , Cyclamen Europaeum ,
mium Iodatum , Cadmium Metallicum , Cadmium Muriati Cydonia Vulgaris, Cynara Scolymus , Cynodon Dactylon,
cum , Cadmium Sulphuratum , Cadmium Sulphuricum , Caf Cypripedium Pubescens, Cysteinum , Cytisus Scoparius ,
feinum , Cahinca , Cajuputum , Caladium Seguinum , 50 Damiana, Daphne Indica, Datura Arborea, Datura Metel,
Calcarea Acetica, Calcarea Arsenicica, Calcarea Car DDT, Delphininum , Derris Pinnata, Dichapetalum , Dictam
bonica , Calcarea Caustica , Calcarea Fluorica , Calcarea nus Albus, Digitalinum , Digitalis Purpurea , Digitoxinum ,
Hypochlorata , Calcarea Hypophosphorosa , Calcarea Dioscorea Villosa , Dioscoreinum , Diphtherinum , Diphthe
Iodata , Calcarea Lactica , Calcarea Muriatica, Calcarea rotozinum , Diptherinum , Diptherotoxinum , Dirca Palustris,
Oxalica , Calcarea Phosphorica, Calcarea Picrata , Calcarea 55 DNA , Dolichos Pruriens, Doryphora Decemlineata , Draba
Silicata, Calcarea Sulphurica, Calendula Officinalis, Cal Vema, Drosera Rotundifolia, DTTAB ( Diptheria ), Duboisia
luna Vulgaris, Flos , Calotropis Gigantea, Caltha Palustris, Myoporoides, Dulcamara, Dulcamara, Flos , Dysentery, E.
Camphora, Camphora Monobromata, Camphoricum Aci Coli, Ear, Labyrinth of ( inner ear ), Ear, Middle , Eberthinum ,
dum , Canchalagua, Candida Albicans, Candida Parapsilo Echinacea Angustifolia, Echinacea Purpurea, Elaeis
sis, Canine Dapp, Cantharidinum , Cantharis, Capsicum , 60 Guineensis , Elaps Corallinus, Elaterium , Embryo Suis ,
Capsicum Annuum , Carbo Animalis , Carbo Vegetabilis, Car Emetinum , Enterotoccinum , Eosinum Natrum , Ephedra Vul
bolicum Acidum , Carboneum , Carboneum Chloratum , Car garis, Epigaea Repens, Epilobium Palustre, Epiphegus Vir
boneum Hydrogenisatum , Carboneum Oxygenisatum , Car giniana , Equisetum Arvense , Equisetum Hyemale, Eranthis
boneum Sulphuratum , Carcinosinum , Cardiospermum , Hyemalis, Erechtites Hieracifolia , Erigeron Canadensis,
Carduus Benedictus, Carduus Marianus, Carpinus Betulus, 65 Eriodictyon Califomicum , Erodium , Eryngium Aquaticum ,
Flos , Cartilago Suis , Carum Carvi, Cascarilla , Cassada, Eryngium Maritimum , Erythraea Centaurium , Eschscholtzia
Castanea Sativa, Flos , Castanea Vesca, Castor Equi, Cas Califomica, Eserinum , Etherum , Ethylicum , Ethylum Nitri
 US 10,806,707 B2
59 60
cum , Eucalyptol , Eucalyptus Globulus, Eugenia Caryophyl Kali Permanganicum , Kali Phosphoricum , Kali Picricum ,
lata , Eugenia Jambosa, Euonymus Atropurpureus, Euony Kali Silicatum , Kali Sulphuricum , Kali Tartaricum , Kali
mus Europaeus, Eupatorium Aromaticum , Eupatorium Telluricum , Kalmia Latifolia , Kamala , Karaka, Karwinskia
Cannabinum , Eupatorium Perfoliatum , Eupatorium Pur Humboldtiana , Kino Australiensis, Kousso , Kreosotum ,
pureum , Euphorbia Amygdaloides, Euphorbia Corollata, 5 Laburnum Anagyroides, Lac Caninum , Lac Defloratum , Lac
Euphorbia Cyparissias, Euphorbia Hypericifolia , Euphor Felinum , Lac Matemum , Lac Vaccinum , Lacerta Agilis,
bia Lathyris, Euphorbia Pilulifera , Euphorbium Offici Lachesis Mutus, Lachnanthes Tinctoria , Lacticum Acidum ,
narum , Euphrasia Officinalis, Eupion, Eyebright herb, Fag Lactuca Virosa, Lamium Album , Lapis Albus, Lappa Major,
opyrum Esculentum , Fagus Sylvatica , Fagus Sylvatica, Flos , Larix Decidua, Flos , Lathyrus Cicera, Lathyrus Sativus,
Fel Tauri, Ferrum Aceticum , Ferrum Arsenicicum , Ferrum 10 Latrodectus Katipo , Latrodectus Mactans, Laurocerasus ,
Bromatum , Ferrum Carbonicum , Ferrum Citricum , Ferrum Lecithin granules, Lecithin potenized, Ledum Palustre,
Cyanatum , Ferrum lodatum , Ferrum Lacticum , Ferrum Lemna Minor, Leonurus Cardiaca, Lepidium Bonariense ,
Metallicum , Ferrum Muriaticum , Ferrum Pemitricum , Fer Leptandra Virginica , Lespedeza Capitata , Levico , Levisti
rum Phosphoricum , Ferrum Picricum , Ferrum Sulphuricum , cum Officinale, Levomepromazinum , Liatris Spicata, Lilium
Ferrum Tartaricum , Ferula Glauca, Ficus Religiosa , Filix 15 Tigrinum , Limulus, Linaria Vulgaris, Linum Catharticum ,
Mas , Foeniculum Vulgare , Folliculinum , Formalinum , For Linum Usitatissimum , Lithium Benzoicum , Lithium Broma
mica Rufa, Formicum Acidum , Fragaria Vesca , Franciscea tum , Lithium Carbonicum , Lithium Muriaticum , Lobelia
Uniflora , Fraxinus Americana, Fraxinus Excelsior, Fuchsi Cardinalis, Lobelia Erinus, Lobelia Inflata, Lobelia Pur
num , Fucus Vesiculosus, Fumaria Officinalis, Fumaricum purescens, Lobelia Syphilitica , Lobelinum , Lolium Temu
Acidum , Funiculus Umbilicalis Suis , Galanthus Nivalis, 20 lentum , Lonicera Caprifolium , Flos , Lonicera Pericly
Galega Officinalis, Galium Aparine, Gallicum Acidum , Gal menum , Lonicera Xylosteum , Lophophytum Leandri,
phimia Glauca , Gambogia, Garlic, Gaultheria Procumbens, Luesinum , Luffa Operculata , Lupulinum , Lycopersicum
Gelsemium Sempervirens, Genista Tinctoria , Gentiana Cru Esculentum , Lycopodium Clavatum , Lycopus Virginicus,
ciata , Gentiana Lutea , Gentiana Quinqueflora , Gentianella Lysimachia Nummularia , Lyssin , Lyssinum , Macrotinum ,
Amarella, Flos , Geranium Maculatum , Geranium Rober- 25 Magnesia Carbonica , Magnesia Muriatica , Magnesia Oxy
tianum , Geum Rivale, Geum Urbanum , Ginkgo biloba , data , Magnesia Phosphorica , Magnesia Sulphurica, Magne
Glandula Suprarenalis Suis , Glechoma Hederacea , Glonoi sium Metallicum , Magnolia Glauca, Magnolia Grandiflora,
num , Glycerinum , Glycogenum , Glycyrrhiza Glabra , Malaria Off., Malus Pumila , Flos , Mancinella , Mandragora
Gnaphalium Leontopodium , Gnaphalium Polycephalum , Officinarum , Manganum Aceticum , Manganum Carboni
Gnaphalium Uliginosum , Gonotoxinum , Gossypium Herba- 30 cum , Manganum Metallicum , Manganum Muriaticum ,
ceum , Granatum , Graphites , Gratiola Officinalis, Grindelia , Manganum Oxydatum Nativum , Manganum Oxydatum
Guaco , Guaiacum , Guarea Trichilioides, Guatteria Gaumeri, Nigrum , Manganum Phosphoricum , Manganum Sulphuri
Gunpowder, Gymnocladus Canadensis, Haematoxylon cum , Mangifera Indica , Marrubium Vulgare, Matico , Mat
Campechianum , Haemophilus Infl. B , Hair Bulb , Pilo Seba thiola Graeca, Medorrhinum (Gonorrheal virus ), Medulla
ceous Zone , Hamamelis Virginiana , Haronga Madagas - car- 35 Ossis Suis , Medusa , Melastoma Ackermani, Melilotus Alba ,
iensis , Hedeoma Pulegioides , Hedera Helix, Hekla Lava , Melilotus Officinalis , Melissa Officinalis, Menispermum
Helianthemum Nummularium , Flos , Helianthus Annuus, Canadense, Mentha Piperita , Mentha Pulegium , Mentha
Heliotropium Peruvianum , Helix Tosta , Helleborus Foeti Viridis, Mentholum , Menyanthes Trifoliata, Mephitis
dus, Helleborus Niger, Helleborus Viridis, Heloderma, Mephitica, Mercurialis Perennis , Mercurius Aceticus, Mer
Helonias Dioica , Hepar Suis , Hepar Sulphuris Calcareum , 40 curius Auratus, Mercurius Bromatus, Mercurius Corrosivus ,
Hepar Sulphuris Kalinum , Hepatica Triloba, Hepatitis A , Mercurius Cum Kali Iodatus, Mercurius Cyanatus, Mercu
Hepatitis B , Hepatitis C , Heracleum Sphondylium , Herpes rius Dulcis, Mercurius Iodatus Flavus, Mercurius Iodatus
Zoster, Hippozaeninum , Hippuricum Acidum , Hirudinum , Ruber, Mercurius Methylenus, Mercurius Nitricus, Mercu
Histaminum Hydrochloricum , Hoang - Nan, Hoitzia Coc rius Praecipitatus Albus, Mercurius Praecipitatus Ruber ,
cinea , Holarrhena Antidysenterica, Homarus, Hottonia 45 Mercurius Solubilis, Mercurius Sulphocyanatus, Mercurius
Palustris, Flos , Humulus Lupulus, Hura Brasiliensis, Hura Sulphuratus Ruber , Mercurius Sulphuricus, Mercurius
Crepitans, Hydrangea Arborescens, Hydrastininum Muri Vivus, Methylene Blue , Mezereum , Millefolium , Mimosa
aticum , Hydrastis Canadensis, Hydrocotyle Asiatica , Pudica, Mimulus Guttatus, Flos , Mitchella Repens,
Hydrocyanicum Acidum , Hydrofluoricum Acidum , Hydro Momordica Balsamina , Mononucleosis, Monotropa Uni
phis Cyanocinctus, Hydrophyllum Virginianum , Hyoscya- 50 flora , Morbillinum (Measles ) , Moschus, Mucosa Nasalis
minum , Hyoscyaminum , Hydrobromatum , Hyoscyamus Suis , Mullein Essence , Murex Purpurea , Muriaticum Aci
Niger,Hypericum Perforatum , Hypothalamus, Iberis Amara , dum , Musa Sapientum , Mygale, Myosotis Arvensis, Myrica
Ichthyolum , Ignatia Amara , Ilex Aquifolium , Ilex Aquifo Cerifera, Myristica Sebifera, Myrrha, Myrtus Communis,
lium , Flos , Ilex Paraguariensis, Illicium Anisatum , Impa Nabalus Serpentarius, Nadidum , Naja Tripudians, Naph
tiens Glandulifera , Flos , Imperatoria Ostruthium , Indigo, 55 thalinum , Narceinum , Narcissus, Pseudo-, Narcissus, Nar
Indium Metallicum , Indolum , Influenzinum , Inula Hele cotinum , Nasturtium Aquaticum , Natrum Arsenicicum ,
nium , Iodium , Iodoformum , Ipecacuanha, Ipomoea Stans, Natrum Bicarbonicum , Natrum Bromatum , Natrum Car
Iridium Metallicum , Iris Florentina, Iris Foetidissima, Iris bonicum , Natrum Fluoratum , Natrum Hypochlorosum ,
Germanica, Iris Tenax, Iris Versicolor, Jacaranda Caroba, Natrum Lacticum , Natrum Muriaticum , Natrum Nitricum ,
Jalapa , Jasminum Officinale, Jasper, Jatropha Curcas, Jatro- 60 Natrum Nitrosum , Natrum Oxalaceticum , Natrum Phos
pha Urens, Jequirity, Jonesia Asoca , Juglans Cinerea, Jug phoricum , Natrum Pyruvicum , Natrum Salicylicum , Natrum
lans Regia, Juglans Regia , Flos , Juncus Effusus, Juniperus Silicofluoricum , Natrum Sulphuratum , Natrum Sulphuri
Communis, Juniperus Virginiana, Justicia Adhatoda, Kali cum , Natrum Sulphurosum , Negundo, Nepenthes , Nepeta
Aceticum , Kali Arsenicosum , Kali Bichromicum , Kali Bro Cataria , Niccolum Carbonicum , Niccolum Metallicum ,
matum , Kali Carbonicum , Kali Causticum , Kali Chloricum , 65 Niccolum Sulphuricum , Nicotinamidum , Nicotinum , Nitri
Kali Chromicum , Kali Cyanatum , Kali Ferrocyanatum , Kali Spiritus Dulcis, Nitricum Acidum , Nitrogenum Oxygena
Iodatum , Kali Muriaticum , Kali Nitricum , Kali Oxalicum , tum , Nitromuriaticum Acidum , Nosode Kit , Nosode - Select
 US 10,806,707 B2
61 62
your own, Nuclear Radiation , Nuphar Luteum , Nux bucus Nigra , Sanguinaria Canadensis, Sanguinarinum
Moschata , Nux Vomica , Nymphaea Odorata , Ocimum Basi Nitricum , Sanicula , Santoninum , Saponaria Officinalis,
licum , Ocimum Canum , Ocimum Sanctum , Oenanthe Cro Saponinum , Sarcode - Select your own organ remedy, Sarco
cata , Oenothera Biennis, Olea Europaea , Flos , Oleander , lacticum Acidum , Sarracenia Purpurea, Sarsaparilla, Sas
Oleum Animale, Oleum Carvi, Oleum Morrhuae , Oleum 5 safras Officinale, Scammonium , Scarlatinum , Secale - Ergot
Ricini , Oleum Santali, Olibanum , Oniscus , Ononis Spinosa , Schinus Molle , Scilla Maritima, Scleranthus Annuus , Flos ,
Onopordum , Onosmodium Virginianum , Oophorinum , Scolopendra , Scolopendrium Vulgare , Scopolaminum Hyd
Opuntia Vulgaris , Orchitinum , Oreodaphne Califomica , robromidum , Scrophularia Nodosa , Scutellaria Lateriflora ,
Origanum Majorana, Omithogalum Umbellatum , Secale Comutum , Secale -Ergot, Sedum Acre , Selenium
Omithogalum Umbellatum , Flos, Oroticum Acidum , Oscil- 10 Metallicum , Sempervivum Tectorum , Senecio Aureus, Sene
lococcinum , Osmium Metallicum , Ostrya , Ova Tosta , Ovi cio Jacobaea , Senega Officinalis, Senna , Sepia, Serum
Gallinae Pellicula , Oxalicum Acidum , Oxalis Acetosella, Anguillae, Serum Anticolibacillaire , Serum De Yersin ,
Oxydendrum Arboreum , Oxytropis Lambertii, Paeonia Offi Serum Equi, Shigella , Silica Marina , Silicea , Silphium Lac
cinalis, Palladium Metallicum , Paloondo , Pancreas Suis , iniatum , Sinapis Alba, Sinapis Arvensis, Flos , Sinapis Nigra,
Pancreatinum , Paraffinum , Parathormonum , Parathyroid, 15 Sinusitisinum , Sium Latifolium , Skatolum , Skookum
Paratyphoidinum B , Pareira Brava , Parietaria Officinalis, Chuck , Slag , Solaninum , Solanum Arrebenta, Solanum
Paris Quadrifolia, Paronichia Illecebrum , Parotidinum Carolinense , Solanum Mammosum , Solanum Nigrum , Sola
(Mumps ), Parthenium , Passiflora Incamata , Pastinaca num Oleraceum , Solanum Tuberosum , Solidago Virgaurea,
Sativa , Paullinia Pinnata , Paullinia Sorbilis, Pecten, Pedicu Sparteinum Sulphuricum , Spigelia Anthelmia, Spigelia
lus Capitis , Penicillinum , Penthorum Sedoides, Pepsinum , 20 Marilandica, Spilanthes Oleracea , Spinacia , Spiraea Ulma
Perhexilinum , Persea Americana , Pertussinum ( Whooping ria, Spiranthes Autumnalis, Spongia Encephalitis, Spongia
Cough) , Petiveria Tetrandra , Petroleum , Petroselinum Sati Tosta , Stachys Betonica , Stannum Iodatum , Stannum Metal
vum , Phallus Impudicus, Phaseolus, Phellandrium Aquati licum , Staphyloccoccus Aureus, Staphylococcinum , Staphy
cum , Phenacetinum , Phenobarbitalum , Phloridzinum , Phos lotoxinum , Staphysagria , Stellaria Media , Sterculia Acumi
phoricum Acidum , Phosphorus, Physalis Alkekenge, 25 nata , Stibium Metallicum , Sticta Pulmonaria, Stigmata
Physotigma Venenosum , Phytolacca Decandra, Pichi , Picri Maidis , Stillingia Sylvatica, Stramonium , Streptococcinum ,
cum Acidum , Picrotoxinum , Pilocarpinum , Pilocarpinum Strontium Bromatum , Strontium Carbonicum , Strontium
Muriaticum , Pilocarpinum Nitricum , Pilocarpus, Pimenta Nitricum , Strophanthus Hispidus, Strophanthus Sarmento
Officinalis, Pimpinella Saxifraga , Pinus Lambertiana , Pinus sus , Strychninum , Strychinum Arsenicicum , Strychinum
Sylvestris, Pinus Sylvestris, Flos , Piper Methysticum , Piper 30 Nitricum , Strychninum Phosphoricum , Strychninum Sul
Nigrum , Piperazinum , Piscidia Erythrina, Pituitarum Poste phuricum , Succinicum Acidum , Succinum , Sulphanilami
rium , Pix Liquida, Placenta Totalis Suis , Plague , Plantago dum , Sulphonalum , Sulphur, Sulphur Hydrogenisatum , Sul
Major, Plata us , Platinum Metallicum , Platinum Muriati phur Iodatum , Sulphuricum Acidum , Sulphurosum Acidum ,
cum , Plectranthus Fruticosus, Plumbago Littoralis, Sumbul, Symphoricarpus Racemosus, Symphytum Offici
Plumbum Aceticum , Plumbum Carbonicum , Plumbum 35 nale , Syphilinum ( Luesinum ), Syzygium Jambolanum ,
Chromicum , Plumbum Iodatum , Plumbum Metallicum , Tabacum , Tamus Communis, Tanacetum Vulgare, Tanghinia
Pneumococcinum , Podophyllinum , Podophyllum Peltatum , Venenifera , Tannicum Acidum , Taraxacum Officinale,
Polio , Polygonum Punctatum , Polygonum Sagittatum , Poly Taraxacum Officinale, Radix , Tarentula Cubensis, Tarentula
porus Officinalis, Polyporus Pinicola , Populus Candicans, Hispana, Tartaricum Acidum , Taxus Baccata , Tellurium
Populus Tremula , Flos , Populus Tremuloides, Potentilla 40 Metallicum , Teplitz , Terebinthina, Tetanotoxinum , Tetra
Anserina , Pothos Foetidus, Primula Obconica , Primula dymite , Teucrium Marum , Teucrium Scorodonia , Thallium
Veris, Primula Vulgaris, Proteus Bulgaris, Proteus Vulgaris, Metallicum , Thaspium Aureum , Thea sinensis, Theobromi
Prunus Cerasifera, Flos , Prunus Padus, Prunus Spinosa , num , Theridion , Thiaminum Hydrochloricum , Thioproper
Prunus Virginiana , Psorinum , Ptelea Trifoliata , Pulex Irri azinum , Thiosinaminum , Thlaspi Bursa -Pastoris, Thuja
tans, Pulsatilla Niger, Pulsatilla Nuttalliana , Pyrethrum 45 Lobbi , Thuja Occidentalis, Thymolum , Thymus Serpyllum ,
Parthenium , Pyridoxinum Hydrochloricum , Pyrogenium Thyroidinum , Tilia Europaea , Titanium Metallicum , Tongo ,
sepsis, Pyrus Americana, Quassia Amara , Quebracho, Tormentilla , Torula Cerevisiae, Toxicophis Pugnax, Trades
Quercus Glandium Spiritus, Quercus Robur, Quercus Robur , cantia Diuretica, Tribulus Terrestris, Trifolium Pratense,
Flos , Quillaja Saponaria , Radium Bromatum , Ranunculus Trifolium Repens, Trillium Pendulum , Trimethylaminum ,
Acris, Ranunculus Bulbosus, Ranunculus Ficaria , Ranun- 50 Triosteum Perfoliatum , Triticum Repens, Tropaeolum
culus Glacialis, Ranunculus Repens, Ranunculus Scelera Majus, Tuberculinum , Tuberculinum Residuum , Tussilago
tus, Raphanus Sativus, Ratanhia, Rauwolfia Serpentina , Farfara , Tussilago Fragrans, Tussilago Petasites , Typhoidi
Reserpinum , Resina Laricis, Resorcinum , Rhamnus Califo num , Ulex Europaeus, Flos , Ulmus Fulva , Ulmus Procera ,
mica , Rhamnus Cathartica, Rhamnus Frangula, Rhamnus Flos , Upas Tieute , Uranium Nitricum , Urea, Uricum Aci
Purshiana , Rheum Officinale, Rhodium Metallicum , Rho- 55 dum , Urtica Crenulata , Urtica Dioica , Urtica Urens, Usnea
dodendron Chrysanthum , Rhus Aromatica , Rhus Diversi Barbata , Ustilago Maidis, Uva - Ursi herb , Uva - Ursi,
loba , Rhus Glabra, Rhus Toxicodendron , Rhus Venenata , V.A.B.-BCG , Vaccinium Myrtillus, Vaccinotoxinum , Valeri
Riboflavinum , Ricinus Communis, RNA, Robinia Pseudoa ana Officinalis, Vanadium Metallicum , Varicella enus Mer
cacia , Rock Water, Rosa Canina, Rosa Canina , Flos , Rosa cenaria (Chicken Pox ) , Variolinum ( Smallpox) , Veratrinum ,
Damascena , Rosmarinus Officinalis, Rubella (German 60 Veratrum Album , Veratrum Nigrum , Veratrum Viride, Ver
Measles ) , Rubeola ( Measles ) , Rubia Tinctorum , Rumex bascum Thapsus, Verbena Hastata, Verbena Officinalis, Ver
Acetosa , Rumex Crispus, Rumex Obtusifolius, Russula Foe bena Officinalis, Flos , Veronica Beccabunga , Veronica Offi
tens , Ruta Graveolens, Sabidilla , Sabal Serrulata, Sabina , cinalis, Vesicaria, Vespa Crabro , Viburnum Opulus,
Saccharinum , Saccharum Lactis, Saccharum Officinale, Viburnum Prunifolium , Vinca Minor, Viloa Odorata , Viola
Salicinum , Salicylicum Acidum , Salix Alba , Salix Nigra , 65 Tricolor, Vipera Berus, Viscum Album , Vitamin B12 , Vita
Salix Purpurea, Salix Vitellina, Flos , Salmonella, Salol , min K , Vitis Vinifera, Flos , Wiesbaden, Wyethia Hele
Salvia Officinalis, Samarskite , Sambucus Canadensis, Sam nioides, X -Ray, Xanthoxylum Fraxineum , Xerophyllum
 US 10,806,707 B2
63 64
Asphodeloides, Yohimbinum , Yucca Filamentosa , Zincum ( w / w ), or from about 30 % ( w / w ) to about 35 % ( w / w ), or
Aceticum , Zincum Bromatum , Zincum Carbonicum , Zin from about 35 % ( w / w ) to about 40 % ( w / w ), or from about
cum Cyanatum , Zincum Gluconicum , Zincum Iodatum , 40% ( w / w ) to about 45 % ( w / w ), or from about 45 % ( w / w )
Zincum Metallicum , Zincum Muriaticum , Zincum Oxyda to about 50% ( w / w ), or from about 50 % ( w / w ) to about 55 %
tum , Zincum Phosphoratum , Zincum Picricum , Zincum 5 (w / w ), or from about 55 % ( w / w ) to about 60% (w / w ), or
Sulphuricum , Zincum Valerianicum , Zingiber Officinale, from about 60 % ( w / w ) to about 65 % ( w / w ), or from about
and combinations thereof. 65 % ( w / w ) to about 70 % ( w / w ), or from about 70 % ( w / w )
Flower essences can also be included in cannabis oil to about 75 % ( w / w ), or from about 75 % ( w / w ) to about 80 %
compositions prepared according to the invention . Examples ( w / w ), or from about 80 % ( w / w ) to about 85 % ( w / w ), or
of suitable flower essences include, but are not limited to , 10 from about 85 % ( w /w ) to about 90 % ( w /w ), or from about
Acacia , Actaea , Agrimony, Alpine Lily, Angel's Trumpet, 90% ( w /w ) to about 95 % ( w / w ). A carrier oil can be present
Aloe Vera, Angelica, Basil , Apricot, Arnica Beech , Aspen , in an amount ranging from about 5 % ( w / w ) to about 95 %
Avocado, Beech , Bee Balm , Black Cohosh , Baby Blue Eyes , ( w / w ), or from about 10 % ( w / w ) to about 90% ( w / w ), or
Black- Eyed Susan, Blackberry , Bloodroot, Calendula , from about 15 % ( w / w ) to about 85 % ( w / w ), or from about
Bleeding Heart,California Fuchsia, California Pitcher Plant, 15 20% ( w / w ) to about 80% ( w / w ), or from about 25 % ( w / w )
Borage, Buttercup , California Wild Rose, California Poppy , to about 75 % ( w / w ), or from about 30 % ( w / w ) to about 70 %
CallaLily, Cerato , Canyon Dudleya , Chamomile , Cayenne , ( w / w ), or from about 35 % ( w / w ) to about 65 % ( w / w ), or
Cedar, Chaparral, Centaury, Centaurium erythraea or Cen from about 40 % ( w / w ) to about 60 % ( w / w ), or from about
taurium umbellatum , Cerato , Cherry Plum , Chestnut Bud , 45 % ( w / w ) to about 55 % ( w / w ).
Corn , Dandelion , Chicory, Cinquefoil, Coffee , Coreopsis, 20 In some embodiments, the invention provides a cannabis
Crab Apple , Chrysanthemum , Clematis, Desert Dandelion, oil composition comprising a cannabis oil ( e.g. , a cannabis
Deerbrush , Cosmos , Dill , Elm , Evening Primrose, Dog oil extract prepared according to the methods described
wood, Easter Lily, Eucalyptus, Fairy Lantern , Echinacea , herein ) and hemp seed oil as a carrier oil . In some such
Fawn Lily, Fig , Filaree, Gentian, Goldenrod, Forget-Me embodiments, the cannabis oil is present in the cannabis oil
Not , Golden Ear Drops, Golden Yarrow , Fuchsia , Garlic , 25 composition in an amount ranging from about 7 % ( w / w ) to
Gorse , Honeysuckle , Heather Hombeam , Hibiscus, about 70 % ( w / w ). In some such embodiments, hemp seed oil
Hound's Tongue, Holly , Impatiens, Indian Paintbrush , is present in the cannabis oil composition in an amount
Larch , Lily , Indian Pink , Larkspur, Iris, Iris douglasiana / Iris ranging from about 30 % ( w / w ) to about 95 % ( w / w ). In some
versicolor, Lady's Slipper, Cypripedium parviflorum /Cy embodiments, the cannabis oil composition further com
pripedium reginae, Lotus, Lavender, Love-Lies-Bleeding, 30 prises vitamin E. In some such embodiments, the ratio of the
Mariposa Lily, Madia , Magnolia, Milkweed , Mallow, hemp seed oil to the vitamin E is around 200 : 1 by weight.
Mimulus, Manzanita, Morning Glory, Motherwort, Moun In some such embodiments, vitamin E is present in the
tain Pennyroyal, Mustard, Mountain Pride, Nasturtium , cannabis oil composition in an amount ranging from about
Mugwort, Nicotiana , Noni , Oak , Olive , Pine , Orange, 0.2 % ( w / w ) to about 0.5 % ( w / w ). The composition contain
Oregon Grape, Pansy, Passion Flower, Pear, Petunia , Pink 35 ing cannabis oil , hemp seed oil , and vitamin E can be
Angel's Trumpet, Pink Monkeyflower, Penstemon , Pink administered orally via a gelatin capsule such as a vegetarian
Yarrow Achillea millefolium var. rubra , Peppermint, Poison gel capsule .
Oak, Pomegranate, Queen Anne's Lace , Pretty Face , In some embodiments, the invention provides a cannabis
Quince , Purple Monkeyflower, Rabbitbrush , Quaking Grass , oil composition comprising about 9 % ( w / w ) cannabis oil ,
Red Chestnut, Red clover, Rescue Remedy, Rock Rose , 40 about 90.5 % ( w /w ) hemp seed oil , and about 0.5 % ( w /w )
Sacred Datura , Sagebrush , Scarlet Pimpemel, Rock Water vitamin E. In some such embodiments, the cannabis oil
Solarized spring water, Saguaro , Rosemary, Rose , Saint composition is formulated in a vegetarian gel capsule for
John's Wort, Scarlet Monkeyflower, Shasta Daisy, Scleran oral administration .
thus, Shooting Star, Scotch Broom , Snapdragon, Squash , In some embodiments, the invention provides a cannabis
Self -Heal , Star of Bethlehem , Star Thistle , Sweet Chestnut , 45 oil composition comprising about 33.3 % ( w / w ) cannabis
Star Tulip, Strawberry, Sun Cup , Sweet Pea , Sticky Mon oil , about 66.4 % ( w / w ) hemp seed oil , and about 0.3 % ( w / w )
keyflower, Tansy, Sunflower, Thyme, Tiger Lily, Trillium , vitamin E. In some such embodiments, the cannabis oil
Violet, Walnut, Trumpet Vine, Vervain , Water Lily, Water composition is formulated in a vegetarian gel capsule for
Violet, Vine, White Chestnut, Wild Oat , Wild Rose , Yellow oral administration .
Star Tulip, Willow , Yerba Santa , Yarrow , Yucca, Zinnia , and 50 In some embodiments, the invention provides a cannabis
combinations thereof. oil composition comprising about 66.7 % ( w / w ) cannabis
In certain embodiments, additional carrier oils are added oil , about 33.2 % ( w / w ) hemp seed oil , and about 0.2 % ( w / w )
to the cannabis oils . Examples of carrier oils include , but are vitamin E. In some such embodiments, the cannabis oil
not limited to , almond oil ; aloe vera oil ; apricot kernel oil ; composition is formulated in a vegetarian gel capsule for
avocado oil ; argan oil ; calendula oil ; carrot seed oil ; castor 55 oral administration .
oil ; coconut oil ; evening primrose oil ; fish oils and oils rich
in omega - 3 fatty acids (e.g. , algae , krill, flaxseed ); grape IV. Pharmaceutical Compositions and Methods of
seed oil ; hazelnut oil ; hemp seed oil ; jojoba oil ; macadamia Administration
oil ; olive oil ; raspberry seed oil ; sesame oil ; sunflower oil ;
walnut oil ; wheatgerm oil , and combinations thereof. 60 The cannabis oil extracts described herein are useful in
When added, a carrier oil will typically be present in an the manufacture of a pharmaceutical composition or a
amount ranging from about 1 % ( w / w ) to about 95 % ( w / w ). medicament for treating a number of conditions including ,
A carrier oil can be present, for example, in an amount but not limited to , cancer, headaches , vertigo , body aches ,
ranging from about 5 % ( w / w ) to about 10 % ( w / w ), or from and glaucoma.
about 10 % ( w / w ) to about 15 % ( w / w ), or from about 15 % 65 Pharmaceutical compositions or medicaments for use in
( w / w ) to about 20 % ( w / w ), or from about 20 % ( w / w ) to the present invention can be formulated by standard tech
about 25 % ( w / w ), or from about 25 % ( w / w ) to about 30 % niques or methods well-known in the art of pharmacy using
 US 10,806,707 B2
65 66
one or more physiologically acceptable carriers or excipi toothpastes. Liquid preparations for oral administration can
ents . Suitable pharmaceutical carriers are described herein be prepared by conventional means with pharmaceutically
and in , e.g. , " Remington's Pharmaceutical Sciences ” by E. acceptable additives, for example, suspending agents, for
W. Martin . Cannabis oil extracts can be formulated for example, sorbitol syrup , cellulose derivatives, or hydroge
administration by any suitable route , including, but not 5 nated edible fats; emulsifying agents , for example, lecithin ,
limited to , orally, topically, nasally, rectally, vaginally, pul xanthan gum , or acacia ; non - aqueous vehicles, for example,
monary, parenterally ( e.g. , intravenously, subcutaneously, almond oil , sesame oil , hemp seed oil , fish oil , oily esters ,
intramuscularly, etc. ), and combinations thereof. In some ethyl alcohol , or fractionated vegetable oils ; and preserva
embodiments, the cannabis oil is diluted in a liquid, e.g. , a tives , for example , methyl or propyl - p -hydroxybenzoates or
carrier oil. will
given case The depend
most suitable
in part route
on theofcondition
administration in any 10 sorbic
being treated acid .The preparations can also contain buffer salts,
flavoring, coloring, and / or sweetening agents as appropriate.
as well as the response of the subject to the particular route Typical formulations for topical administration include
of treatment. creams, ointments , sprays , lotions , hydrocolloid dressings,
In certain embodiments, cannabis oil compositions as
described herein are administered via a vaporizer or like 15 and patches, as well as eye drops, ear drops, and deodorants.
device as described , for example, in U.S.Pat . No.8,915,254 ; Cannabis oils can be administered via transdermal patches
as described , for example, in U.S. Pat . Appl. Pub . No.
U.S. Pat . Appl. Pub . No. 2014/0060552 ; U.S. Pat . No.
8,488,952 ; and U.S. Pat . Appl. Pub . No. 2015/0040926 . 2015/0126595 and U.S. Pat. No. 8,449,908 . Formulation for
Compositions for pulmonary administration also include , rectal or vaginal administration is also contemplated. The
but are not limited to, dry powder compositions consisting 20
of the powder of a cannabis oil described herein , and the
cannabis oils can be formulated, for example, as supposi
tories containing conventional suppository bases such as
powder of a suitable carrier and / or lubricant. The composi cocoa butter and other glycerides as described in U.S. Pat.
tions for pulmonary administration can be inhaled from any Nos . 5,508,037 and 4,933,363 . Compositions can contain
suitable dry powder inhaler device known to a person skilled other solidifying agents such as shea butter, beeswax , kokum
in the art . In certain instances, the compositions may be 25 butter, mango butter, ilipe butter, tamanu butter, carnauba
conveniently delivered in the form of an aerosol spray from wax , emulsifying wax , soy wax , castor wax , rice bran wax ,
pressurized packs or a nebulizer, with the use of a suitable and candelila wax . Compositions can further include clays
propellant, for example, dichlorodifluoromethane, trichloro (e.g. , Bentonite, French green clays , Fuller's earth , Rhassoul
fluoromethane, dichlorotetrafluoroethane, carbon dioxide , or clay, white kaolin clay ) and salts ( e.g. , sea salt , Himalayan
other suitable gas . In the case of a pressurized aerosol , the 30 pink salt , and magnesium salts such as Epsom salt) .
dosage unit can be determined by providing a valve to The compositions set forth herein can be formulated for
deliver a metered amount. Capsules and cartridges of, for parenteral administration by injection, for example by bolus
example, gelatin for use in an inhaler or insufflator can be injection or continuous infusion . Formulations for injection
formulated containing a powder mix of the compound ( s) and can be presented in unit dosage form , for example, in
a suitable powder base , for example, lactose or starch . 35 ampoules or in multi - dose containers, optionally with an
For oral administration , a pharmaceutical composition or added preservative. Injectable compositions are preferably
a medicament can take the form of, e.g. , a tablet or a capsule aqueous isotonic solutions or suspensions , and suppositories
prepared by conventional means with a pharmaceutically are preferably prepared from fatty emulsions or suspensions.
acceptable excipient. Preferred are tablets and gelatin cap The compositions may be sterilized and / or contain adju
sules comprising the active ingredients ), together with ( a ) 40 vants , such as preserving, stabilizing, wetting or emulsifying
diluents or fillers, e.g. , lactose , dextrose , sucrose , mannitol, agents, solution promoters, salts for regulating the osmotic
maltodextrin , lecithin , agarose, Xanthan gum , guar gum , pressure, buffers, and / or other ingredients. Alternatively, the
sorbitol, cellulose (e.g. , ethyl cellulose , microcrystalline compositions can be in powder form for reconstitution with
cellulose ), glycine, pectin, polyacrylates and/ or calcium a suitable vehicle , for example, a carrier oil , before use . In
hydrogen phosphate, calcium sulfate, ( b ) lubricants, e.g. , 45 addition, the compositions may also contain other therapeu
silica , anhydrous colloidal silica , talcum , stearic acid , its tic agents or substances . The compositions are prepared
magnesium or calcium salt (e.g. , magnesium stearate or according to conventional mixing , granulating or coating
calcium stearate ), metallic stearates, colloidal silicon diox methods, respectively, and contain about 0.1 to 75 % , pref
ide , hydrogenated vegetable oil , corn starch , sodium benzo erably about 1 to 50 % , of the cannabis oils .
ate , sodium acetate and / or polyethyleneglycol; for tablets 50 In general, subjects receiving a cannabis oil composition
also ( c ) binders, e.g. , magnesium aluminum silicate , starch orally are administered doses ranging from 1-2000 mg of
paste, gelatin , tragacanth , methylcellulose , sodium car cannabis oil . A small dose ranging from 1-20 mg will
boxymethylcellulose , polyvinylpyrrolidone and / or hydroxy typically be administered orally when treatment of a condi
propyl methylcellulose ; if desired (d) disintegrants, e.g. , tion such as cancer is initiated , and the dose will be increased
starches (e.g. , potato starch or sodium starch) , glycolate , 55 (e.g. , doubled) over a period of days or weeks until the
agar, alginic acid or its sodium or potassium salt , or effer maximum dose is reached . As a non - limiting example, a
vescent mixtures; ( e) wetting agents, e.g. , sodium lauryl cannabis oil of the invention can be administered orally to
sulfate, and / or ( f) absorbents, colorants, flavors and sweet a cancer patient at a dose of about 15 mg on days 1-4 of a
eners. Tablets can be either uncoated or coated according to treatment program ; then at a dose of about 30 mg on days
methods known in the art. The excipients described herein 60 5-8 of the treatment program ; then at a dose of about 60 mg
can also be used for preparation of buccal dosage forms and on days 9-12 of the treatment program ; then at a dose of
sublingual dosage forms (e.g. , films and lozenges) as about 125 mg on days 13-16 of the treatment program ; then
described , for example , in U.S. Pat . Nos . 5,981,552 and at a dose of about 250 mg on days 17-20 of the treatment
8,475,832 . Formulation in chewing gums as described, for program ; then at a dose of about 500 mg on days 21-24 of
example, in U.S. Pat. No. 8,722,022 , is also contemplated . 65 the treatment program ; then at a dose of about 1000 mg on
Further preparations for oral administration can take the day 25 and subsequent days of the treatment program .
form of, for example, solutions , syrups, suspensions, and Further doses ( typically in the range of about 1-5 mg of
 US 10,806,707 B2
67 68
cannabis oil per dose ) can be administered via vaporizer from about 30 : 1 to about 1:30 . The THC : CBD weight ratio
throughout the treatment program . can range from about 25 : 1 to about 1:25 , or from about 20 : 1
Increasing doses can be administered using vegetarian gel to about 1:20 , or from about 16 : 1 to about 1:16 , or from
capsule formulations as described herein . As a non- limiting about 12 : 1 to about 1:12 , or from about 8 : 1 to about 1 : 8 , or
example , gel capsules with a 15 - mg dose ofcannabis oil can 5 from about 6 : 1 to about 1 : 6 , or from about 5 : 1 to about 1 : 5 ,
be administered on the first 12 days of treatment ( 1 , 2 , or 4 or from about 4 : 1 to about 1 : 4 , or from about 2 : 1 to about
capsules per day ; capsule fill containing 9 % (w /w ) cannabis 2 : 1 . In some embodiments , a blended cannabis oil compo
oil , 90.5 % ( w / w ) hemp seed oil , 0.5 % ( w / w ) vitamin E) . sition for treatment of an immune condition will have a
Then , gel capsules with a 120 -mg dose of cannabis oil can THC : CBD weight ratio of about 4 : 1 . In some embodiments,
be administered on days 17-20 of treatment ( 1 or 2 capsules 10 a blended cannabis oil composition for treatment of an
per day; capsule fill containing 33.3 % ( w / w ) cannabis oil , immune condition will have a THC : CBD weight ratio of
66.4 % ( w / w ) hemp seed oil , 0.3 % ( w / w ) vitamin E ) . Gel about 1 : 2 . In some embodiments , a blended cannabis oil
capsules with a 480 - mg dose of cannabis oil can be admin composition for treatment of an immune condition will have
istered on days 21-28 of treatment ( 1 or 2 capsules per day ; a THC : CBD weight ratio of about 1:20 .
capsule fill containing 66.7 % ( w / w ) cannabis oil , 33.2 % 15 Blended cannabis oil compositions for treatment of con
( w / w ) hemp seed oil , 0.2 % ( w / w ) vitamin E) . From day 29 ditions affecting the nervous system ( e.g. , neurodegenerative
onward, two gel capsules with a 480 - mg dose of cannabis disorders, epilepsy, pain , sleep , anxiety, psychological dis
oil can be administered ( capsule fill containing 66.7 % ( w / w ) orders) will typically have THC : CBD weight ratios ranging
cannabis oil , 33.2 % ( w / w ) hemp seed oil , 0.2 % ( w / w ) from about 30 : 1 to about 1:30 . The THC : CBD weight ratio
vitamin E) . Further doses can be administered via vaporizer 20 can range from about 25 : 1 to about 1:25, or from about 20 : 1
throughout the treatment program . to about 1:20 , or from about 16 : 1 to about 1:16 , or from
about 12 : 1 to about 1:12 , or from about 8 : 1 to about 1 : 8 , or
V. Blended Cannabis Oil Compositions from about 6 : 1 to about 1 : 6 , or from about 5 : 1 to about 1 : 5 ,
or from about 4 : 1 to about 1 : 4 , or from about 2 : 1 to about
In another aspect , the invention provides cannabis oil 25 2 : 1 . In some embodiments, a blended cannabis oil compo
compositions having a defined profile of cannabinoids and / sition for treatment of a condition affecting the nervous
or terpenes, which compositions are prepared by blending system will have a THC : CBD weight ratio of about 4 : 1 . In
two or more of the cannabis oils described above . The some embodiments , a blended cannabis oil composition for
cannabinoid profile refers to the number, identity, and quan treatment of a condition affecting the nervous system will
tity of cannabinoids in a cannabis oil ; likewise , the terpene 30 have a THC : CBD weight ratio of about 1 : 1 . In some
profile refers to the number, identity , and quantity of ter embodiments, a blended cannabis oil composition for treat
penes in the oil . Blending of two or more cannabis oils can ment of a condition affecting the nervous system will have
provide compositions with properties and effects that are a THC : CBD weight ratio of about 1:20 .
distinct from cannabis oils prepared from single cannabis A blended cannabis oil composition of the invention can
strains . As a non - limiting example, breast cancer patients 35 contain any ratio of cannabinoids and / or terpenes suitable
can benefit from administration of CBD and CBG . A com for treating a given indication or otherwise eliciting a
position containing a cannabinoid profile, designed to ben desired response in a subject to whom the composition is
efit a particular breast cancer patient population , can be administered . For example, blended cannabis oil composi
obtained by blending complementary cannabis oils prepared tions containing predominantly neutral cannabinoids will
from a cannabis strain rich in CBD ( see , e.g. , Gallily, et al . 40 typically have THC : THCA : CBD : CBDA weight (or molar )
Pharmacology & Pharmacy, 2015 ; 6 : 75-85 ) and a cannabis ratios ranging from about 20 : 0.01 : 1 : 0.01 to about 1 : 0.01 :
strain rich in CBG ( see , e.g. , Fournier, et al . Planta Med 20 : 0.01 . Blended cannabis oil compositions containing pre
1987 ; 53 (3 ) : 277-280 ) . Furthermore, the aggregate proper dominantly acidic cannabinoids will typically have THC :
ties of terpene mixtures can contribute meaningfully to the THCA : CBD : CBDA weight ( or molar) ratios ranging from
entourage effects of cannabis - based medicinal extracts in 45 about 0.01 : 20 : 0.01 : 1 to about 0.01 : 1 : 0.01 : 20 . The THC :
the treatment of conditions such as pain , inflammation , THCA : CBD : CBDA weight ratio can range from about 8 : 1 :
depression , anxiety, addiction , epilepsy, cancer ( including, 1 : 0.1 to about 1 : 0.1 : 8 : 1 ; or from about 6 : 1 : 1 : 0.1 to about
but not limited to , breast cancer and glioblastoma) , fatty 1 : 0.1 : 6 : 1 ; or from about 4 : 1 : 1 : 0.1 to about 1 : 0.1 : 4 : 1 ; or from
cysts , fungal infections, and bacterial infections. See , e.g. , about 2 : 1 : 1 : 0.1 to about 1 : 0.1 : 2 : 1 ; or from about 1 : 10 : 1 : 10
Russo , et al . British Journal of Pharmacology, 2011 ; 163 : 50 to about 1 : 10 : 0.1 : 1 ; or from about 1 : 4 : 0.1 : 1 to about 0.1 : 1 :
1344-1364 . 1 : 4 . In certain embodiments , the blended cannabis oil com
A blended cannabis oil composition of the invention can position has a THC : THCA : CBD : CBDA weight ratio of
contain any ratio of cannabinoids and / or terpenes suitable about 4 : 1 : 1 : 0.1 .
for treating a given indication or otherwise eliciting a Blended oil compositions having a high amount of THC
desired response in a subject to whom the composition is 55 (e.g. , a composition with a THC : CBD ratio around 20 : 1 ) can
administered . For example, blended cannabis oil composi be particularly useful for treating severe pain and inflam
tions for treatment of cancer will typically have THC : CBD mation , as well as for inducing sleep . Blended oil compo
weight ratios ranging from about 10 : 1 to about 1:10 . The sitions with a THC : CBD ratio around 4 : 1 can also have
THC : CBD weight ratio can range from about 8 : 1 to about sleep - inducing and anti - inflammatory effects. The anti-in
1 : 8 , or from about 6 : 1 to about 1 : 6 , or from about 5 : 1 to 60 flammatory effect has been observed to increase cumula
about 1 : 5 , or from about 4 : 1 to about 1 : 4 , or from about 2 : 1 tively over time in certain subjects.
to about 2 : 1 . In some embodiments, a blended cannabis oil Blended oil compositions with high levels of THC can
composition for treatment of cancer will have a THC :CBD induce or impair cognition in certain subjects, particularly in
weight ratio of about 4 : 1 . the absence of gradual attenuation of the subject to the
Blended cannabis oil compositions for treatment of 65 neurological effects of the compositions. Blended oil com
immune conditions (e.g. , Lyme disease , autoimmune con positions with a THC : CBD ratio around 2 : 1 can therefore be
ditions ) will typically have THC : CBD weight ratios ranging used to elicit the strong effects of high - THC compositions
 US 10,806,707 B2
69 70
while preventing anxiety or other side effects due to the ing a first cannabis oil preparation having a first cannabinoid
softening effect of CBD . CBD can also enhance the effects profile, a second cannabis oil preparation having a second
of THC in a synergistic fashion . In certain oncology patients cannabinoid profile , and a third cannabis oil preparation
and seizure patients, blended oil compositions with a THC : having a third cannabinoid profile.
CBD ratio around 1 : 1 can provide symptom relief with less 5 In certain embodiments, the invention provides a compo
cognitive alteration than blended oil compositions with a sition containing a first cannabis oil preparation having a
THC : CBD ratio around 4 : 1 . first cannabinoid profile , a second cannabis oil preparation
Blended oil compositions with a THC : CBD ratio around having a second cannabinoid profile, a third cannabis oil
1 : 2 can be useful for treating patients who require THC for preparation having a third cannabinoid profile, and a fourth
effective symptom mood
want to experience control-altering
, but cannot
effectstolerate or do notor 10 cannabis oil preparation having a fourth cannabinoid profile.
( e.g. , euphoria
anxiety ) associated with high - THC compositions. Depend In some such embodiments, at least 50 % of the total THC in
ing on the particular subject, a THC : CBD ratio around 1 : 2 the composition is present in the combined cannabinoid
can be sleep - inducing, calming , mood - elevating, and mildly profile of the first cannabis oil preparation , the second
to moderately appetite - inducing. Blended oil compositions 15 cannabis oil preparation , and the third cannabis oil prepa
with a THC : CBD ratio around 1 : 4 can be used to incorporate ration; and at least 50 % of the total CBD in the composition
THC as an anti- inflammatory agent that contributes syner is present in the fourth cannabinoid profile . In some such
gistically to alleviation of symptoms by CBD . embodiments, at least 75 % of the total THC in the compo
Blended oil compositions having a high amount of CBD sition is present in the combined cannabinoid profile of the
(e.g. , a composition with a THC : CBD ratio around 1:20) can 20 first cannabis oil preparation , the second cannabis oil prepa
be useful for treating inflammation as well as chronic pain ration , and the third cannabis oil preparation; and at least
including , but not limited to , back pain, fibromyalgia, and 75 % of the total CBD in the composition is present in the
migraine. In certain instances , high- CBD blended oil com fourth cannabinoid profile .
positions can be used to supplement or replace NSAIDs or In some embodiments, the invention provides a compo
other over -the - counter pain medications and anti- inflamma- 25 sition containing four cannabis oil preparations wherein the
tory medications. High - CBD compositions can also be used first cannabis oil preparation is prepared from a Cannabis
to reduce anxiety, enhance serotonin , and elicit focusing indica strain , the second cannabis oil preparation is prepared
and / or calming effects . The anti - spasticity properties of from a Cannabis sativa strain, the third cannabis oil prepa
high - CBD blended oil compositions also makes them useful ration is prepared from the Blueberry cannabis strain, and
for treating Parkinson's disease , Huntington's disease , mul- 30 the fourth cannabis oil preparation is prepared from the
tiple sclerosis, and other neurodegenerative diseases . AC/DC cannabis strain . In some such embodiments, the
In some embodiments, the blended cannabis oil compo ratio of the first, second, third, and fourth cannabis oil
sition includes a CBD component derived from the AC/DC preparations is about 55 : 10 : 10 : 25 by weight. In some such
cannabis strain and a THC component derived from a embodiments, the composition further contains vitamin E.
Cannabis sativa strain . In some embodiments, the blended 35 In some such embodiments , the ratio of the first, second ,
cannabis oil composition includes an oil prepared from the third , and fourth cannabis oil preparations is about 60:10 :
AC/DC cannabis strain , as describe herein , and an equal part 10:20 by weight. In some such embodiments, the THC : CBD
( i.e. , 1 : 1 w/w ) of oil prepared from a Cannabis sativa strain ratio is around 4 : 1 .
( e.g. , Infinite Euphoria ). In some such embodiments, the In some such embodiments, the ratio of the first, second ,
blended cannabis oil composition further includes vitamin 40 third , and fourth cannabis oil preparations is about 45:10 :
E. In some such embodiments, the blended cannabis oil 10:35 by weight. In some such embodiments , the THC : CBD
composition further includes vitamin E and one or more ratio is around 2 : 1 .
essential oils selected from bergamot essential oil , blood In some such embodiments, the ratio of the first, second ,
orange essential oil , sweet orange essential oil , lemon essen third , and fourth cannabis oil preparations is about 30:10 :
tial oil , vanilla essential oil , neroli essential oil , lemongrass 45 10:50 by weight. In some such embodiments , the THC : CBD
essential oil , lavender essential oil , peppermint essential oil , ratio is around 1 : 1 .
and spearmint essential oil . In some such embodiments, the In some such embodiments, the ratio of the first, second ,
blended cannabis oil composition further includes vitamin E third, and fourth cannabis oil preparations is about 10:10 :
and one or more essential oils selected from bergamot 10:70 by weight. In some such embodiments, the THC : CBD
essential oil , blood orange essential oil , neroli essential oil , 50 ratio is around 1 : 2 .
peppermint essential oil , and spearmint essential oil . In some such embodiments, the ratio of the first, second ,
In some embodiments, the blended cannabis oil compo third, and fourth cannabis oil preparations is about 5 : 5 : 5 : 85
sition includes a CBD component derived from the Medi by weight. In some such embodiments, the THC : CBD ratio
haze cannabis strain and a THC component derived from a is around 1 : 4 .
Cannabis indica strain . In some embodiments, the blended 55 In some embodiments , the first cannabis oil composition
cannabis oil composition includes an oil prepared from the is prepared from a Cannabis indica strain selected from the
Medihaze cannabis strain and an equal part ( i.e. , 1 : 1 w/ w ) group consisting of Gorilla Glue ; Blueberry Smooth , Black
of oil prepared from a Cannabis indica strain (e.g. , Afghan Lime Reserve, Purple Trainwreck , Dream Kush, Blue Die
Goo , Prize Kush, or Blueberry ). In some such embodiments , sel , Purple OG , Skywalker OG , Dream Kush , Nepalese
the blended cannabis oil composition further includes vita- 60 Kush , Purple Hashplant, Bubble Gum , MK Ultra , Prize
min E. In some such embodiments , the blended cannabis oil Kush , Chocolate Kush , Afgoo, OG Kush , Afghan Kush ,
composition further includes vitamin E and one or more Blueberry Kush , Blackberry Kush , Bubba Kush, Northern
essential oils selected from lavender essential oil and lem lights, Granddaddy purple, OG -Kush , and Girl Scout Cook
ongrass essential oil . ies .
The blended cannabis compositions can further contain 65 In some embodiments, the first cannabis oil composition
additional cannabis oil preparations. In some embodiments , prepared from the Cannabis indica strain contains THC in
for example, the invention provides a composition contain an amount ranging from about 45 % to about 90 % ( e.g. , from
 US 10,806,707 B2
71 72
49.7 % to 86.35 % ) and CBD in an amount ranging up to up to about 0.2 % ( e.g. , from 0.0 % to 0.14 % ) ; and / or
about 5.0% ( e.g. , from 0.0% to 3.57 % ) . terpinolene in an amount ranging up to about 0.05 % ( e.g. ,
In some embodiments, the first cannabis oil composition from 0.0 % to 0.002 %% ) ; and / or isobomeol in an amount
further contains linalool in an amount ranging from about ranging from about 0.05 % to about 0.50 % (e.g. , from 0.09 %
0.001 % to about 0.005 % ( e.g. , from 0.001 % to 0.36 % ) ; 5 to 0.15 % ) ; and /or guaiol in an amount ranging from about
and / or ß - caryophyllene in an amount ranging from about 0.001 % to about 0.500% ( e.g. , from 0.004 % to 0.29 % ) ;
0.001 % to about 2.5 % ( e.g. , from 0.002 % to 1.22 % ) ; and / or and / or geraniol in an amount ranging up to about 0.5 % ( e.g. ,
caryophyllene oxide in an amount ranging from about from 0.0 % to 0.003 % ) ; and / or y -terpinene in an amount
0.001 % to about 1.0 % (e.g. , about 0.002 % to 0.66 % ) ; and / or ranging from about 0.001 % to about 0.005 % ( e.g. , from
a -humulene in an amount ranging from about 0.01 % to 10 0.001 % to 0.002 % ) ; and / or cis - ocimene in an amount rang
about 1 % ( e.g. , from 0.02 % to 0.49 % ) ; and /or a -bisabolol in ing from about 0.001 % to about 0.050 % (e.g. , from 0.002 %
an amount ranging from about 0.005 % to about 0.5 % ( e.g. , to 0.013 % ) ; and / or camphene in an amount ranging up to
from 0.008 % to 0.43 % ) ; and / or a - terpineol in an amount about 0.002 % (e.g. , from 0.0 % to 0.001 % ) ; and / or B -pinene
ranging from about 0.05 % to about 0.5 % (e.g. , from 0.08 % in an amount ranging from about 0.001 % to about 0.250 %
to 0.15 % ) ; and / or endo - fenchyl alcohol in an amount rang- 15 (e.g. , from 0.001 % to 0.157 % ) ; and / or j3 -myrcene in an
ing from about 0.04 % to about 0.4 % (e.g. , from 0.06 % to amount ranging from about 0.001 % to about 0.500 % ( e.g. ,
0.15 % ) ; and / or terpinolene in an amount ranging from about from 0.002 % to 0.19 % ) ; and / or a - terpinene in an amount
0.001 % to about 0.025 % ( e.g. , from 0.001 % to 0.01 % ) ; ranging from about 0.001 % to about 0.050% ( e.g. , from
and / or guaiol in an amount ranging from about 0.001 % to 0.001 % to 0.002 % ) ; and /or a -pinene in an amount ranging
about 1.0 % (e.g. , from 0.007 % to 0.33 % ) ; and / or cis- 20 from about 0.001 % to about 0.100 % (e.g. , from 0.002 % to
ocimene in an amount ranging from about 0.001 % to about 0.023 % ) ; and / or 3 - carene in an amount ranging from about
0.05 % (e.g. , from 0.005 % to 0.014 % ) ; and / or 1 -pinene in an 0.001 % to about 0.100 % (e.g. , from 0.003 % to 0.016 % ) ;
amount ranging from about 0.005 % to about 0.1 % ( e.g. , and / or ß -eudesmol in an amount ranging up to about 0.250 %
from 0.021 % to 0.049 % ) ; and / or J -myrcene in an amount (e.g. , from 0.0 % to 0.18 % ) ; and /or a - eudesmol in an amount
ranging from about 0.001 % to about 0.01 % ( e.g. , from 25 ranging from about 0.05 % to about 1.0 % (e.g. , from 0.09 %
0.003 % to 0.006 % ) ; and / or a - terpinene in an amount rang to 0.53 % ) ; and / or y -eudesmol in an amount ranging from
ing from about 0.001 % to about 0.01 % (e.g. , from 0.001 % about 0.1 % to about 0.5 % ( e.g. , from 0.14 % to 0.24 % ) ;
to 0.002 % ) ; and / or a -pinene in an amount ranging from and / or valencene in an amount ranging from about 0.1 % to
about 0.001 % to about 0.01 % (e.g. , from 0.002 % to about 0.5 % ( e.g. , from 0.1 % to 0.16 % ) ; and / or cis - neridol in
0.007 % ) ; and / or 3 - carene in an amount ranging up to about 30 an amount ranging from about 0.005 % to about 1.0 % ( e.g. ,
0.005 % ( e.g. , from 0 % to 0.003 % ) ; and /or ß -eudesmol in an from 0.01 % to 0.56 % ) ; and / or trans - neridol in an amount
amount ranging from about 0.005 % to about 0.250 % ( e.g. , ranging from about 0.001 % to about 0.500% ( e.g. , from
from 0.09 % to 0.16 % ) ; and / or a - eudesmol in an amount 0.003 % 0.22 % ) ; and / or isopulegol in an amount ranging
ranging from about 0.050 % to about 0.500% (e.g. , from from about 0.001 % to about 0.100 % (e.g. , from 0.005 % to
0.12 % to 0.32 % ) ; and / or y -eudesmol in an amount ranging 35 0.048 % ) ; and / or cymene in an amount ranging from about
from about 0.050 % to about 0.500 % ( e.g. , from 0.07 % to 0.001 % to about 0.050 % (e.g. , from 0.003 % to 0.036 % ) .
0.29 % ) ; and / or cis - neridol in an amount ranging from about In some embodiments, the third cannabis oil composition
0.1 % to about 1.0 % (e.g. , from 0.15 % to 0.58 % ) ; and / or prepared from the Blueberry cannabis strain contains THC
trans - neridol in an amount ranging from about 0.001 % to content in an amount ranging from about 50 % to about 85 %
about 0.100 % (e.g. , from 0.002 % to 0.06 % ) ; and / or isop- 40 (e.g. , from 530.98 % to 81.4 % ) and CBD in an amount
ulegol in an amount ranging from about 0.01 % to about ranging from about 0.1 % to about 10 % ( e.g. , from 0.14 % to
0.10 % ( e.g. , from 0.016 % to 0.054 % ) ; and / or cymene in an 60.63 % ).
amount ranging from about 0.001 % to about 0.050 % ( e.g. , In some embodiments, the third cannabis oil composition
from 0.004 % to 0.025 % ) . further contains linalool in an amount ranging from about
In some embodiments, the second cannabis oil composi- 45 0.001 % to about 0.200% ( e.g. , from 0.001 % to 0.15 % ) ;
tion is prepared from a Cannabis sativa strain selected from and / or ß -caryophyllene in an amount ranging from about
the group consisting of Sour Boggle , Blue Dream , Jilly 0.001 % to about 1.0% (e.g. , from 0.002 % to 0.77 % ) ; and /or
Bean , Infinite Euphoria , Dream Queen, Sour Diesel , and caryophyllene oxide in an amount ranging from about
Green Crack . 0.001 % to about 0.200% ( e.g. , from 0.002 % to 0.16 % ) ;
In some embodiments, the second cannabis oil composi- 50 and / or a -humulene in an amount ranging from about
tion prepared from the Cannabis sativa strain contains THC 0.001 % to about 0.75 % (e.g. , from 0.001 % to 0.49 % ) ;
in an amount ranging from about 45 % to about 85 % ( e.g. , and / or a -bisabolol in an amount ranging from about 0.005 %
from 49.6 % to 81.4 % ) and CBD in an amount ranging up to to about 0.5 % ( e.g. , from 0.011 % to 0.25 % ) ; and / or a -ter
about 2.5 % ( e.g. , from 0.0% to 1.9 % ) . pineol in an amount ranging up to about 0.1 % (e.g. , from
In some embodiments, the second cannabis oil composi- 55 0.0 % to 0.07 % ) ; and / or terpinolene in an amount ranging up
tion further contains linalool in an amount ranging from to about 0.001 % (e.g. , from 0.0 % to 0.001 % ) ; and / or isobor
about 0.001 % to about 1.0 % (e.g. , from 0.001 % to 0.93 % ) ; neol in an amount ranging up to about 0.2 % ( e.g. , from 0.0 %
and / or ß -caryophyllene in an amount ranging from about to 0.15 % ) ; and / or guaiol in an amount ranging up to about
0.001 % to about 2.0 % ( e.g. , from 0.002 % to 0.96 % ) ; and / or 0.01 % ( e.g. , from 0.0 % to 0.009 % ) ; and / or geraniol in an
caryophyllene oxide in an amount ranging from about 60 amount ranging up to about 0.005 % ( e.g. , from 0.0 % to
0.001 % to about 0.050% ( e.g. , from 0.002 % to 0.42 % ) ; 0.003 % ) ; and / or cis -ocimene in an amount ranging from
and / or a -humulene in an amount ranging from about about 0.001 % to about 0.01 % (e.g. , from 0.003 % to
0.001 % to about 1.0 % ( e.g. , from 0.001 % to 0.49 % ) ; and / or 0.007 % ) ; and / or camphene in an amount ranging up to about
a -bisabolol in an amount ranging from about 0.001 % to 0.01 % ( e.g. , from 0.0% to 0.009 % ) ; and / or B -pinene in an
about 2.5 % ( e.g. , from 0.005 % to 1.17 % ) ; and / or a - terpineol 65 amount ranging from about 0.001 % to about 0.1 % ( e.g. ,
in an amount ranging up to about 0.1 % (e.g. , from 0.0 % to from 0.001 0.065 % ) ; and / or B -myrcene in an amount rang
0.07 % ) ; and / or endo - fenchyl alcohol in an amount ranging ing from about 0.001 % to about 0.01 % (e.g. , from 0.001
 US 10,806,707 B2
73 74
0.009 % ) ; and / or a -terpinene in an amount ranging up to Medi - Haze , Charlotte's Web, Sour Tsunami, Abacus,
about 0.005 % ( e.g. , from 0.0 % to 0.001 % ) ; and /or a -pinene Ringo's Gift, Cannatonic , Harlequin , and Buddha Passion
in an amount ranging from about 0.001 % to about 0.01 % for preparation of the fourth cannabis oil composition .
( e.g. , from 0.002 % to 0.007 % ) ; and / or 3 - carene in an The blended oil compositions can be diluted with sun
amount ranging from about 0.001 % to about 0.01 % ( e.g. , 5 flower oil or another suitable carrier oil , as described above ,
from 0.003 % to 0.008 % ) ; and / or a - eudesmol in an amount to provide a diluted cannabis oil composition. For example,
ranging up to about 0.1 % (e.g. , from 0.0% to 0.09 % ) ; and /or a diluted cannabis oil composition can contain total can
cis -neridol in an amount ranging from about 0.01 % to about nabinoids in amounts ranging from about 500 mg to about
0.5 % (e.g. , from 0.043 % to 0.19 % ) ; and /or trans - neridol in
an amount ranging from about 0.001 % to about 0.05 % ( e.g. , 10 20,000 mg in volumes ranging from about 0.5 fluid ounces
from 0.003 % to 0.012 % ) ; and /or isopulegol in an amount cannabismLoil) tocomposition
( i.e. , 15 about 2.5 fluid ounces (i.e. , 75 mL ) . A diluted
can contain the total cannabinoids
ranging from about 0.001 % to about 0.1 % (e.g. , from in a concentration of around
0.005 % to 0.048 % ) ; and /or cymene in an amount ranging 100 mg /mL , or 300 mg/mL . 10Themgoil /mL , or 20 mg /mL , or
concentrate can be
from about 0.001 % to about 0.2 % ( e.g. , from 0.003 % to further diluted with sunflower oil or another suitable carrier
0.173 % ) . 15
In some embodiments , the Blueberry strain can be oil by a factor of 2 , 5 , 10 , 25 , or higher to aid oral
replaced with a strain selected from the group consisting of administration of a desired dose .
When added , a carrier oil will typically be present in a
Blackberry Kush , Bubba Kush , Northern lights, Granddaddy
purple, OG -Kush , Sour Diesel , Blue Dream , Green Crack , diluted cannabis oil composition in an amount ranging from
and Girl Scout Cookies for preparation of the third cannabis 20 about 1 % ( w / w ) to about 95 % (w /w ).A carrier oil can be
oil composition . present, for example, in an amount ranging from about 5 %
In some embodiments , the fourth cannabis oil composi ( w / w ) to about 10 % ( w / w ), or from about 10% ( w / w ) to
tion prepared from the ACDC cannabis strain contains THC about 15 % ( w / w ), or from about 15 % ( w / w ) to about 20 %
in an amount ranging from about 1 % to about 40 % ( e.g. , ( w / w ), or from about 20 % ( w / w ) to about 25 % ( w / w ), or
from 20.4-370.60 % ) and CBD in an amount ranging from 25 from about 25 % ( w / w ) to about 30 % ( w / w ), or from about
about 25 % to about 75 % (e.g. , from 29.10-72.60% ) . 30% ( w / w ) to about 35 % ( w / w ), or from about 35 % ( w / w )
In some embodiments, the fourth cannabis oil composi to about 40% ( w / w ), or from about 40 % ( w / w ) to about 45 %
tion further contains linalool in an amount ranging from ( w / w ), or from about 45 % ( w / w ) to about 50% ( w / w ), or
about 0.01 % to about 0.25 % (e.g. , from 0.042 % to 0.19 % ) ; from about 50 % ( w / w ) to about 55 % ( w / w ), or from about
and / or ß -caryophyllene in an amount ranging from about 30 55 % ( w /w ) to about 60 % ( w / w ), or from about 60 % ( w / w )
0.05 % to about 1.0 % (e.g. , from 0.086 % to 0.66 % ) ; and / or to about 65 % ( w / w ), or from about 65 % ( w / w ) to about 70 %
caryophyllene oxide in an amount ranging from about ( w / w ), or from about 70 % ( w / w ) to about 75 % ( w / w ), or
0.001 % to about 0.5 % ( e.g. , from 0.002 % to 0.42 % ) ; and /or from about 75 % ( w / w ) to about 80 % ( w / w ), or from about
a -humulene in an amount ranging from about 0.01 % to 80% ( w / w ) to about 85 % ( w / w ), or from about 85 % ( w / w )
about 0.5 % (e.g. , from 0.028 % to 0.38 % ) ; and/or a - bisab- 35 to about 90% ( w / w ), or from about 90 % ( w / w ) to about 95 %
olol in an amount ranging from about 0.005 % to about 1.0 % ( w / w ). A carrier oil can be present in an amount ranging
( e.g. , from 0.011 % to 0.99 % ) ; and / or a - terpineol in an from about 5 % ( w / w ) to about 95 % ( w / w ), or from about
amount ranging from about 0.05 % to about 0.2 % (e.g. , from 10% ( w / w ) to about 90 % ( w / w ), or from about 15 % ( w / w )
0.08 % to 0.1 % ) ; and / or endo - fenchyl alcohol in an amount to about 85 % ( w / w ), or from about 20 % ( w / w ) to about 80 %
ranging from about 0.05 % to about 0.2 % (e.g. , from 0.08 % 40 ( w /w ), or from about 25 % ( w / w ) to about 75 % ( w / w ), or
to 0.1 % ) ; and / or isobomeol in an amount ranging from about from about 30 % ( w / w ) to about 70 % ( w / w ), or from about
0.05 % to about 0.5 % ( e.g. , from 0.08 % to 0.2 % ) ; and / or 35 % ( w / w ) to about 65 % ( w / w ), or from about 40 % ( w / w )
guaiol in an amount ranging from about 0.005 % to about to about 60% ( w / w ), or from about 45 % ( w / w ) to about 55 %
0.5 % (e.g. , from 0.01 % to 0.28 % ) ; and / or geraniol in an ( w / w ).
amount ranging up to about 0.2 % (e.g. , from 0.0 % to 45
0.102 % ) ; and / or B - pinene in an amount ranging from about VI . Examples
0.005 % to about 0.05 % (e.g. , from 0.015 % to 0.028 % ) ;
and / or B -myrcene in an amount ranging from about 0.001 % The following examples are offered to illustrate, but not
to about 0.1 % (e.g. , from 0.002 % to 0.06 % ) ; and /or a - ter to limit , the claimed invention .
pinene in an amount ranging up to about 0.01 % ( e.g. , from 50
0.0 % to 0.005 % ) ; and / or a -pinene in an amount ranging Example 1. Compositions Containing Cannabis Oil
from about 0.001 % to about 0.05 % (e.g. , from 0.002 % to and Vitamin E
0.02 % ) ; and / or B - eudesmol in an amount ranging from about
0.01 % to about 0.2 % ( e.g. , from 0.06 % to 0.12 % ) ; and / or Reference is now made to FIG . 2 , which is a graph
a - eudesmol in an amount ranging from about 0.05 % to 55 depicting the viscosities of cannabis oil compositions as a
about 0.5 % (e.g. , from 0.08 % to 0.43 % ) ; and / or valencene function of vitamin E percentage in the cannabis oil com
in an amount ranging from about 0.05 % to about 0.2 % ( e.g. , positions , according to one or more embodiments . As indi
from 0.08 % to 0.18 % ) ; and / or cis -neridol in an amount cated in FIG . 2 , the viscosities of the cannabis oil compo
ranging from about 0.01 % to about 1.0% ( e.g. , from 0.027 % sitions can be measured in centipoise (CP ) and the % w/w of
to 0.63 % ) ; and / or trans - neridol in an amount ranging from 60 vitamin E 121 can be based on the total weight of the
about 0.01 % to about 0.5 % ( e.g. , from 0.015 % to 0.22 % ) ; cannabis oil composition .
and / or isopulegol in an amount ranging from about 0.01 % to FIG . 2 depicts the results of viscosity experiments con
about 0.1 % (e.g. , from 0.012 % to 0.075 % ) ; and / or cymene ducted by mixing vitamin E 121 of various quantities with
in an amount ranging from about 0.01 % to about 0.05 % the extract 119. In these experiments , the total weight of the
( e.g. , from 0.011 % to 0.025 % ) . 65 cannabis oil composition was set at approximately 25.00
In some embodiments, the ACDC strain can be replaced grams. As indicated in Table 2 below , the % w/w of the
with a strain selected from the group consisting of Harle Tsu , vitamin E 121 ranged from 10% w/w to 50 % w / w :
 US 10,806,707 B2
75 76
TABLE 2 less than 3000 cP . In yet another preferred embodiment, the
cannabis oil composition has a viscosity of less than 2000
Amounts of Vitamin E and Extract in Cannabis Oil Composition cP .
Used in Viscosity Experiments
All cannabis oil compositions were subjected to organo
Total cannabis Oil 5 leptic analysis after addition of vitamin E , including tests
Vitamin E Vitamin E Amount
( grams)
Extract
Amount ( grams)
Composition
( grams)
designed to ascertain the taste , smell , and ease of inhaling
% w /w the cannabis oil compositions. Based on this organoleptic
10 2.50 22.50 25.00 analysis, it was discovered that vitamin E concentrations
20 5.00 20.00 25.00 around 30 % w / w provide useful viscosity levels while
30
40
7.50
10.00
17.50
15.00
25.00
25.00
10 preserving the gustatory or aromatic qualities of the extract
50 12.50 12.50 25.00 119 .
Reference is now made to FIG . 3 , which is a graph
depicting THC and CBD perc ges in cannabis oil com
The purpose of the experiments was to determine a positions made from three strains of cannabis plant material
preferred quantity of vitamin E 121 that will reduce the 15 103 , according to one or more embodiments. In one embodi
viscosity of the cannabis oil composition yet preserve the ment, the cannabis oil composition can be made from
gustatory or aromatic qualities of the extract 119. Moreover, cannabis plant material comprising the AC/ DC cannabis
the preferred quantity of vitamin E 121 should also provide strain . In another embodiment, the cannabis oil composition
beneficial reductions in viscosity while also not displacing can be made from cannabis plant material comprising the
too much of the cannabinoids 107 in the cannabis oil 20 Blueberry cannabis strain . In yet another embodiment, the
composition . cannabis oil composition can be made from cannabis plant
As indicated above , cannabis oil is often highly viscous , material
The
comprising the Cannatonic cannabis strain .
three cannabis strains were selected for their varying
making it difficult to work with and load into new delivery levels of cannabinoids
devices such as vaporizers, E -cigarettes, or pens. As will be 25 was selected to represent107. For example, the AC/DC strain
discussed in more detail below, one unexpected benefit of CBD and low levels of THC . Alsostrains
cannabis with high levels of
, for example, the Blue
mixing the vitamin E 121 with the extract 119 is reducing the berry strain was selected to represent cannabis strains with
viscosity of the cannabis oil composition and making the high levels
cannabis oil composition conducive for loading or packing example, theof THC and low levels of CBD . Also yet another
Cannatonic strain was selected to represent
into modern day vaporizers, E -cigarettes, or pens .
The viscosity experiments were conducted using a vis 30 cannabis strains with moderate levels CBD and THC .
As depicted in FIG . 3 , cannabis oil compositions of
cometer. For example, the viscometer can be a falling ball uniform volume were made with 30% w / w vitamin E 121
viscometer. More specifically, the falling ball viscometer can mixed with extract 119 produced from the AC/ DC , Blue
be a PDVdi - 120 Portable Falling Ball Viscometer from berry, and Cannatonic strains of cannabis plant material 103 .
Stony Brook
sitions ScientificTM
were first heated on.Each of the atcannabis
a hotplate oil compo-
approximately 95 ° 35 UV
These cannabis oil compositions were assayed using HPLC
C. and mixed with a glass stirring rod . Aliquots of the cannabis to determine the level of cannabinoids 107 in each
cannabis oil compositions were then transferred into the performedoiloncomposition . In addition , HPLC -UV was also
extracts with no vitamin E 121 to determine
falling ball viscometer and falling times in seconds were the level of cannabinoids 107 in such extracts.
measured at 45.8 ° C. Results of the viscosity experiments 40 While the mixing of extract 119 with vitamin E 121
are presented in Table 3 below : reduced the amount of cannabinoids detected in the canna
TABLE 3 bis oil compositions relative to the extracts, 30 % w/w of
vitamin E 121 was discovered to reduce the viscosity of the
Falling Times as a Functions of Vitamin E % w /w cannabis oil compositions made from all three strains.
45 Moreover, organoleptic analysis of each cannabis oil com
Vitamin E % w / w Falling Times ( s )
ViscosityFalling
( CP ) , calculated
Times
using position revealed that 30 % w / w of vitamin E 121 also did
not have an adverse effect on the aromatic or gustatory
10
20
333.1
272.1
5868.73
4794.00
qualities of the extract 119 in the cannabis oil composition .
30
40
200.9
159.3
3539.56
2806.63
50 Example 2. Preparation of Cannabis Oils
50 83.7 1474.67
Preparation of AC/DC Cannabis Oil .
2 pounds of cannabis plant material ( strain : AC/DC ) and
The viscosity of each cannabis oil composition was 17 L of EtOH were stored for 24 hours at -18 ° C. prior to
calculated using the applicable falling time in Table 3 above 55 extraction . The 2 pounds were split between two colanders ,
and Equation 1 below : which were placed on top of a collection vessel . 3 L of cold
EtOH was poured evenly over each pound ( 6 L total ) of plant
Viscosity ( CP ) =9.1463 cm - s - 2 * (Needle Density - Ap material and collected in the vessel below . After dripping for
proximate Fluid Density ) * Falling Time Equation 1 :
about 2 min , the resulting solution was poured over the same
In the above Equation 1 , Needle Density=2.9263 g * cm- 3 60 plant material and collected and repeated 3 more times ( 5
and Approximate Fluid Density = 1.0000 g * cm - 3. As shown total pours for over each colander ). After the final pour, the
in FIG . 2 and Table 3 above , an increase in the % w / w of material was left to drip into the collection vessel for 20 min .
vitamin E 121 by 10 % corresponds to an approximate 1000 The solution was then filtered through the Chemex filter
cP decrease in the viscosity of the cannabis oil composition . ( ~ 300 micron ) into a collection flask . This combined solu
In one preferred embodiment, the cannabis oil composi- 65 tion was poured into a glass jar with airtight lid and left
tion has a viscosity of less than 3500 CP . In another preferred underneath a plasma light source for 15 minutes and then
embodiment, the cannabis oil composition has a viscosity of stored at -18 ° C. for 20 hours (Batch 2-1A) .
 US 10,806,707 B2
77 78
The plant material from the colanders was placed in a Preparation of Blueberry Cannabis Oil .
5 - gallon bucket and covered with 11 L of cold EtOH and left 2 pounds of cannabis plant material ( strain : Blueberry )
to sit for 2 hours . The material was filtered through the and 14 L of EtOH were stored for 24 hours at -18 ° C. prior
Chemex filter into a collection flask . This combined solution to extraction. The 2 pounds were split between two colan
was poured into a glass jar with airtight lid and left under- 5 ders , which were placed on top of a collection vessel . 3 L of
neath a plasma light source for 18 hours . Following expo cold EtOH was poured evenly over each pound ( 6 L total )
sure to light, the solution was stored at -18 ° C. for 20 hours of plant material and collected in the vessel below. After
( Batch 2-1B ) . dripping for about 2 min, the resulting solution was poured
The 2-1A and 2-1B batches were independently filtered over the same plant material and collected and repeated 4
while cold through a Buchner funnel with Whatman 150 mm 10 more times ( 6 total pours for over each colander ). After the
filter paper in place into a filtration flask while under vessel for ,20theminmaterial
final pour
. The
was left to drip into the collection
solution was then filtered through the
vacuum . The 2-1A batch solution was split between two Chemex filter ( ~300 micron) into
Megahome distillers . The distillers were turned on and the combined solution was poured into aa collection glass jar with
flask . This
airtight
distillate was collected in a glass receiving vessel
complete. Upon completion, the oil in the distiller was
until 15 lid and then stored at -18 ° C. for 20 hours ( 2 - A )
.
immediately transferred into a tared, glass petri dish and 5 - gallon The plant material from the colanders was placed in a
placed into the vacuum oven at 46 ° C. and 27 mbar for 87 to sit forbucket and covered with 8 L of cold EtOH and left
2 hours . The material was filtered through the
hours ( the pressure was checked daily and brought back Chemex filter into a collection flask . This combined solution
down to 21-27 mbar as necessary ). The 2-1B batch solution 20 was poured into a glass jar with airtight lid and left under
was split between three Megahome distillers . The distillers neath a plasma light source for 18 hours . Following expo
were turned on and the distillate was collected in a glass sure to light, the solution was stored at -18 ° C. for 20 hours
receiving vessel until complete . Upon completion , the oil in (2-3B ) .
the distiller was immediately transferred into a tared , glass The A and B batches were independently filtered while
petri dish and placed into the vacuum oven at 44 ° C. and 22 25 cold through a Buchner funnel with Whatman 150 mm filter
mbar for 120 hours . paper in place into a filtration flask while under vacuum . The
Preparation of Prize Kush Cannabis Oil . 2 -A batch solution was split between two Megahome dis
3 pounds of cannabis plant material ( strain : Prize Kush ) tillers. The distillers were turned on and the distillate was
and 21 L of EtOH were stored for 24 hours at -18 ° C. prior collected in a glass receiving vessel until complete. Upon
to extraction . The 3 pounds were split between three colan- 30 completion, the oil in the distiller was immediately trans
ferred into a tared, glass petri dish and placed into the
ders, which were placed on top of a collection vessel . 3 L of vacuum
cold EtOH was poured evenly over each pound ( 9 L total ) pressure was oven at 46 ° C. and 21 mbar for 143 hours ( the
of plant material and collected in the vessel below . After mbar as necessary checked daily and brought back down to 21-27
). The 2-3B batch solution was split
dripping for about 2 min, the resulting solution was poured 35 between four Megahome
over the same plant material and collected and repeated 4 turned on and the distillate was distillers. The distillers were
more times ( 6 total pours for over each colander ). After the vessel until complete. Upon collected in a glass receiving
final pour, the material was left to drip into the collection distiller was immediately transferred into a,tared
completion the oil in the
, glass petri
vessel for 20 min . The solution was then filtered through the dish and placed into the vacuum oven at 47 ° C. and 27 mbar
Chemex filter ( ~300 micron) into a collection flask . This 40 for 69 hours.
combined solution was poured into a glass jar with airtight
lid and then stored at -18 ° C. for 20 hours ( 2-2A) . Example 3. Preparation of Cannabis Oils
The plant material from the colanders was placed in a
5 - gallon bucket and covered with 12 L of cold EtOH and left A first extract (Extract A) is prepared according to the
to sit for 2 hours . The material was filtered through the 45 following procedure.
Chemex filter into a collection flask . This combined solution Ethanol ( EtOH ; Alchemical Solutions organic 190 proof
was poured into a glass jar with airtight lid and left under neutral grain wheat spirits ) and raw cannabis material are
neath a plasma light source for 18 hours. Following expo stored in at -10 ° C. for at least 16 hr, or for other time
sure to light, the solution was stored at -18 ° C. for 20 hours
periods as necessary . In certain instances , ethanol and raw
(2-2B) . 50 cannabis material are used at room temperature. Three liters
The 2-2A and 2-2B batches were independently filtered of alcohol are typically used for each pound of raw cannabis
while cold through a Buchner funnel with Whatman 150 mm material.
filter paper in place into a filtration flask while under Cold ( or room temperature) raw cannabis material is
vacuum . The 2-2A batch solution was split between two placed in a tabletop Buchner funnel (18 " -36 " diameter;
Megahome distillers . The distillers were turned on and the 55 Bel- Art) with perforated , creped white cellulose filter paper
distillate was collected in a glass receiving vessel until in place . Ethanol -compatible tubing ( e.g. , Tyron Tyoprene)
complete . Upon completion, the oil in the distiller was is attached to the funnel drain spigot and connected to a
immediately transferred into a tared , glass petri dish and collection media bottle . 1-3 lb of raw cannabis material is
placed into the vacuum oven at 47 ° C. and 27 mbar for 72 typically used with an 18 " funnel; 2-5 lb of raw cannabis
hours (the pressure was checked daily and brought back 60 material is typically used with a 24 " funnel; and 4-10 lb of
down to 21-27 mbar as necessary ). The 2-2B batch solution cannabis material is typically used with a 36 " funnel. 1-5 L
was split between four Megahome distillers. The distillers (e.g. , 3 L ) of EtOH per lb of raw cannabis material is poured
were turned on and the distillate was collected in a glass evenly over the raw cannabis material while collecting the
receiving vessel until complete . Upon completion , the oil in ethanolic eluate (“ menstruum ” ) in a 2-5 L media bottle . The
the distiller was immediately transferred into a tared , glass 65 menstruum is collected and re -poured over the cannabis
petri dish and placed into the vacuum oven at 47 ° C. and 27 material 3-6 times . Re-pouring is stopped before the men
mbar for 72 hours . struum turns from yellow - toned to green .
 US 10,806,707 B2
79 80
If necessary, the menstruum is filtered through a Chemex an appropriate funnel ( 6 : 1 ratio, i.e. , 600 g silica to 100 g
300 -um mesh stainless steel filter into a Chemex glass flask . oil ) , which is positioned on a vacuum flask , and is wetted
The menstruum is then transferred to a media bottle with with EtOH . The homogenized oil ( optionally with silica gel)
screw cap and stored at -10 ° C. for no less than 18 hr. is placed on top of the silica pad and is pulled using light
A second extract ( Extract B ) is prepared using the mate 5 vacuum until all solution is absorbed on silica . EtOH is
rial (“ marc” ) remaining from the procedure described above . gently poured ( 1000 mL for 100 g of oil ) on top of the silica
Marc from extract A remains in the tabletop Buchner funnel, gel and is pulled through with vacuum . The filtrate is
and the funnel spigot is closed to prevent draining. Cold (or collected in a flask and concentrated on the rotavapor at 40 °
room temperature ) EtOH is poured over the marc remaining
in the Buchner funnel in an amount sufficient to completely 10 bath temperature and 100 mbar vacuum pressure .
C.
cover the marc (e.g. , 4-5 L per lb ) . The marc is soaked The extract is placed into an Across International Vacuum
( “ macerated ” ) for a period of time typically ranging from a oven set to 46 ° C./115° F. for no less than 12 hours and no
few minutes to about 6 hours ( e.g. , 2 hr ). If raw cannabis more than 5 days . Alternatively , the extract is transferred to
material is used without prior preparation of Extract A , the appropriate glass vials for use with a Buchi multivapor
maceration step is typically conducted for less than 1 hr 15 apparatus set to 50-70 ° C. under reduced pressure between
( e.g. , less than 15 min) . After the maceration step , the funnel 10-100 mbar for a specified time . Once excess EtOH has
valve is opened to separate the marc from the menstruum been fully evaporated , the extract is organoleptically ana
and menstruum is collected in a suitable vessel . lyzed for determination of complete EtOH removal.
The menstruum is filtered through a Chemex mesh stain
less steel filter into a Chemex glass flask ( this step is optional 20 Example 4. Cannabinoid and Terpene Content of
depending upon amount of marc in menstruum after mac Cannabis Oils
eration step ). The filtered menstruum is poured from the
Chemex flask back into a media bottle with screw cap .
For extract B , menstruum , while still in media bottles , is The cannabinoid content and terpene content of cannabis
optionally exposed to sunlight (~ 2 hr) or plasma light (~ 8-10 25 oils prepared according to the methods of the invention was
hr ). Menstruum is solarized until the nettle green color shifts studied . Cannabinoid content was determined using liquid
to yellow brown . After solarization, the media bottle is chromatography /mass spectrometry ( LC - MS ) , and terpene
placed into a freezer for 18-48 hr. content was determined using gas chromatography with
An appropriately sized cellulose filter paper is placed on flame ionization detection ( GC - FID ) .
top of the perforated filter of the table - top Buchner funnel. 30
The vacuum tubing, which is connected to a vacuum , is TABLE 4
attached to the Buchner funnel. Menstruum , that is still in
the media bott is removed from freezer. The filter paper is Neutral cannabinoid content of cannabis oils
wetted using EtOH . The vacuum pump is turned on and the
valve is opened to the vacuum pump . Just before the extract 35 Cannabinoid content (mg
is poured into the funnel, approximately half of an inch of Cannabis cannabinoid per gram oil )
EtOH is poured into the funnel quickly followed by the Example Strain THC CBD CBG CBN
extract.
The filtered menstruum is poured into an evaporation 2-1A AC / DC 26.3 642.4 28.8 0.7
flask (no more than -60 % full ). A B - 491 rotavapor is 40 2-1B AC / DC 38.4 594.1 28.5 1.2
prepared by turning on the heating bath to around 50 ° C. 2-2A Prize Kush 675.6 1.0 38.7 2.5
( e.g. , 20-60 ° C. ) and turning on a F - 105 recirculating chiller 2-2B Prize Kush 655.6 5.0 32.6 14.9
with the water temperature set to 5º C. The evaporation flask 2-3A Blueberry 790.4 7.8 11.7 8.5
is secured to the R - 215 rotavapor and the rotation rate of the ?? AC/ DC
Afghan
26.3 659.8 24.6 0.9
evaporation flask is set to around 150 rpm ( e.g. , 30-300 45 4B
Goo
686.6 10.8 30.1 13.4
rpm ). The vacuum gradient is initiated using a V - 855 5A Blackberry 773.8 10.7 22.5 9.2
vacuum controller. The vacuum pressure is maintained Kush
around 125 mbar (e.g. , 50-300 mbar ). Once the liquid has 6A Blue Diesel 744.5 26.4 27.7 6.8
stopped condensing, the vacuum is released and the rotation 50 7A Buddha 282.1 484.8 17.7 3.1
of the evaporation flask is slowly stopped . Passion
The resulting oil from the evaporation step is optionally SA Cannatonic 366.0 319.9 15.5 18.5
transferred to an appropriately sized round -bottom flask (no 9A Cannatonic 25.8 668.2 18.9 0.3
more than ~ 40 % full) with a magnetic stir bar in the oil for 10A Cannatonic 186.2 525.1 30.7 2.9
heating . The round - bottom flask containing the evaporated 11A Girl Scout
Cookies
677.2 0.0 59.0 6.3
oil /liquid is placed in an OptiTherm reaction block on an 55 12B Harle OG 31.2 659.3 26.1 2.8
IKA stirring hotplate . A condenser is attached to the top of 13A Harle Tsu 200.4 539.4 34.3 3.0
the round - bottom flask and the recirculating chiller is turned 14A Harlequin 209.1 569.2 29.9 2.2
on . Once the condenser is turned on, stirring and heating is 14B Harlequin 171.3 511.2 24.8 6.1
initiated . The oil is heated at around 120 ° C. ( e.g. , 60-150 ° 15A Infinite 745.0 11.8 48.4 9.1
C. ) for between 5 min and 24 hr ( e.g. , 1 hr) depending on the 60 Euphoria
temperature of the heating block and the desired ratio of 16A Medihaze 214.7 493.7 31.8 3.3
acidic to neutral cannabinoids. 17B Medihaze 217.7 450.2 24.5 5.9
Prior to optional silica gel filtration , the oil is homog 18B Prize Kush 707.8 5.1 34.9 11.4
enized in EtOH ( or EtoAc or Heptane; 1 : 2 ratio , i.e. , 100 g 19A Sour Kush 737.7 12.4 16.2 5.9
oil to 200 mL solvent). Around 2 parts silica gel is combined 65 19A Blueberry 639.1 24.2 13.3 13.5
with 1 part oil /EtOH mixture, and the resulting slurry is
concentrated on a rotavapor. A silica gel pad is prepared in
 US 10,806,707 B2
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TABLE 5 TABLE 5 - continued
Acidic cannabinoid content of cannabis oils Acidic cannabinoid content of cannabis oils
Cannabinoid content 5
Cannabinoid content
Cannabis (mg cannabinoid per gram oil) Cannabis (mg cannabinoid per gram oil )
Example Strain THCA CBDA CBGA Example Strain THCA CBDA CBGA
2-1A AC/DC 0.0 2.6 0.0 11A Girl Scout Cookies 0.0 0.0 0.0
2-1B AC / DC 0.0 8.2 0.0 9.2 1.6
2-2A Prize Kush 0.0 0.0 0.0
12B Harle OG 0.0
10 13A Harle Tsu 0.0 45.9 3.8
2-2B Prize Kush 0.0 0.0 1.8
2-3A Blueberry 0.0 0.5 0.0 14A Harlequin 0.0 4.1 1.1
?? AC / DC 0.0 5.7 0.0 14B Harlequin 0.0 10.5 1.4
4B Afghan Goo 3.3 0.3 4.4 15A Infinite Euphoria 0.0 0.0 1.3
SA Blackberry Kush 1.9 0.3 2.9 16A Medihaze 0.0 1.2 2.6
6A Blue Diesel 2.8 0.4 6.6 15 17B
16B Medihaze 0.0 10.4 0.0
?? Buddha Passion 0.0 25.6 1.9 Prize Kush 6.2 0.3 6.6
SA Cannatonic 0.0 2.6 0.8 18A Sour Kush 2.2 0.3 3.0
9A Cannatonic 0.0 0.2 2.3 19A Blueberry 4.4 2.9 4.0
10A Cannatonic 0.0 1.8 0.0

TABLE 6
Terpene content of cannabis oils
Terpene content mg
( terpene per gram oil )
Example B -myrcene linalool Q -terpineol B -caryophyllene a - humulene valencene cis - nerolidol
2-1A 0.0 2.0 0.0 8.1 4.3 0.0 2.3
2-1B 0.0 1.8 0.0 5.7 2.9 0.0 1.8
2-2A 0.6 0.6 0.0 5.3 1.8 0.0 8.1
2-2B 0.0 3.2 0.0 4.3 1.7 0.0 0.0
2-3A 0.0 3.4 1.6 9.2 5.2 1.3 5.5
?? 0.0 1.6 0.0 5.3 2.4 0.0 1.3
4B 0.0 4.0 0.0 2.9 0.0 0.0 0.0
5A 0.0 2.2 0.0 5.4 2.6 0.0 3.4
6A 0.0 2.9 0.0 10.6 4.4 0.0 3.2
7A 0.0 2.6 0.0 4.7 1.8 0.0 4.0
8A 0.0 0.0 0.0 0.0 0.0 0.0 0.0
9A 0.0 1.9 0.0 6.1 3.3 0.0 2.2
10A 0.0 2.0 0.0 5.3 3.1 0.0 1.9
11A 0.0 0.0 0.0 2.6 1.1 0.0 3.3
12A 0.0 0.0 0.0 0.0 0.0 0.0 0.0
13A 0.0 2.3 0.0 4.7 2.4 0.0 1.5
14A 0.0 2.1 0.0 4.9 2.2 0.0 1.7
14B 0.0 1.9 0.0 4.6 2.0 0.0 0.0
15A 0.0 2.5 0.0 4.5 1.8 0.0 2.6
16A 0.0 1.7 0.0 5.0 2.8 0.0 3.2
16B 0.0 2.1 0.0 2.9 1.5 0.0 0.0
17B 0.0 3.7 0.0 3.7 1.4 0.0 0.0
18A 0.0 2.4 0.0 8.8 4.4 0.0 5.5
19A 0.0 3.2 0.0 7.5 4.2 0.0 4.8

TABLE 7
Terpene content of cannabis oils
Terpene content (mg terpene per gram oil)
caryophyllene other
Example oxide guaiol y - eudesmol B -eudesmol a - eudemsol a - bisabolol terpenoids TOTAL
2-1A 1.3 6.8 1.7 3.8 4.9 10.2 16.9 62.3
2-1B 3.0 3.9 1.6 3.4 4.7 8.0 6.0 42.6
2-2A 0.0 0.0 0.0 0.0 0.0 0.9 1.2 18.6
2-2B 1.7 0.0 0.0 0.0 2.6 0.0 0.0 13.6
2-3A 0.0 0.0 0.0 0.0 0.0 0.0 14.2 40.4
?? 0.0 6.8 1.7 3.9 4.9 8.3 15.9 52.1
4B 0.0 1.5 0.0 0.0 4.0 0.0 1.7 14.2
5A 0.0 2.3 0.0 0.0 1.7 0.0 10.4 . 27.9
6A 0.0 2.2 0.0 0.0 1.6 0.0 8.7 33.6
7A 0.0 0.0 0.0 0.0 0.0 0.0 6.0 19.2
8A 0.0 2.1 0.0 0.0 1.4 1.9 4.1 9.4
 US 10,806,707 B2
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TABLE 7 - continued
Terpene content of cannabis oils
Terpene content mg
( terpene per gram oil).
caryophyllene other
Example oxide guaiol y -eudesmol B - eudesmol a - eudemsol a - bisabolol terpenoids TOTAL
9A 0.0 0.0 0.0 0.0 1.7 0.0 3.2 18.4
10A 0.0 3.1 0.0 1.4 2.3 2.6 9.8 31.6
11A 0.0 0.0 0.0 0.0 0.0 1.6 0.0 8.6
12A 0.0 2.1 0.0 1.5 2.8 0.0 2.1 8.5
13A 0.0 2.3 0.0 0.0 2.0 2.4 7.6 25.1
14A 0.0 3.2 0.0 1.6 1.9 2.5 9.5 29.6
14B 1.8 2.4 0.0 0.0 3.2 2.5 4.1 22.5
15A 0.0 2.5 0.0 0.0 2.3 0.0 6.4 22.6
16A 0.0 2.3 0.0 0.0 2.1 1.7 8.6 27.4
16B 0.0 0.0 0.0 0.0 3.9 0.0 1.5 11.9
17B 0.0 0.0 0.0 0.0 2.1 0.0 0.0 10.9
18A 0.0 0.0 0.0 0.0 0.0 2.2 7.8 31.0
19A 1.7 0.0 0.0 0.0 1.5 0.0 8.9 31.8

20
Example 5. Activation of Cannabinoid Receptors cyclic adenosine monophosphate ( CAMP ) . CNR1 activation
by Cannabis Oils of the Invention and CNR2 activation by cannabis oils of the invention were
characterized using a cAMP competitive inhibition immu
Activation of cannabinoid receptors CNR1 ( also known noassay (HitHunter® , DiscoverRX ). In the assay, a fragment
asof the
CB1invention
) and CNR2 (also known as CB2) by cannabis oils 25 B - galactosidase ( B - gal) enzyme donor ( ED) is conjugated
was characterized using a ß -arrestin GPCR with CAMP, which competes with cellular cAMP ( resulting
assay ( PathHunter® , DiscoverRX ) specific for either recep from GPCR activation) for binding to an anti -CAMP anti
tor. The assay is a cell - based functional assay that quanti body. When GPCR activation results in high levels of
tatively measures GPCR activation through ß -arrestin cellular CAMP, the anti -CAMP antibody becomes saturated
recruitment to activated GPCRs . GPCR activity is moni- 30 allowing for the ED - CAMP complex to complement with the
tored by detecting the interaction of B - arrestin with the B - gal acceptor (EA) . The complex forms an active enzyme
activated GPCR using B - galactosidase ( B - gal ) enzyme frag that hydrolyzes a substrate to produce a chemiluminescent
ment mplementation. cts of the assay are described , signal that is directly proportional to the amount of CAMP in
for example, in U.S. Pat . Nos . 6,342,345 ; 7,135,325 ; and the cells . Aspects of the assay are described , for example, in
8,101,373 . Activation of CNR1 and CNR2 by cannabis oils 35 U.S. Pat . Nos . 4,708,929 ; 4,956,274 ; 5,244,785 ; 5,444,161 ;
prepared according to the methods was observed using the 5,604,091 ; and 5,643,734 . Activation of CNR1 and CNR2
B -arrestin assay, as summarized in Table 8 below. by cannabis oils prepared according to the methods was
Activation of CNR1 and CNR2 is accompanied by the observed using the cAMP assay, as summarized in Table 8
release of second messenger signaling molecules including below .
TABLE 8
GPCR Activation Activity of Cannabis Oils Determined
by Cell-Based Assays
CNR1 CNR2

CAMP EC50 B -Arrestin CAMP EC50 B -Arrestin

Example Strain (UM ) EC50 (UM ) (UM ) EC50 (UM )
THC Control 0.00102 3.05 0.279 > 16.7
11 -OH Control 0.0108 > 16.7 0.571 > 16.7
THC
CBD Control > 16.7 > 16.7 > 16.7 > 16.7
2-1A AC /DC 0.117 > 16.7 > 16.7 > 16.7
2-1B AC / DC 0.0791 > 16.7 > 16.7 > 16.7
2-2A Prize Kush 0.00661 > 16.7 0.245 > 16.7
2-2B Prize Kush 0.00572 > 16.7 0.199 > 16.7
2-3A Blueberry 0.00311 > 16.7 0.252 > 16.7
?? AC/DC 0.159 > 16.7 > 16.7 > 16.7
4B Afghan Goo 0.00453 > 16.7 0.193 > 16.7
5A Blackberry Kush 0.00459 1.63 0.308 > 16.7
6A Blue Diesel 0.00527 > 16.7 0.277 > 16.7
7A Buddha Passion 0.0114 > 16.7 > 16.7 > 16.7
SA Cannatonic 0.0111 > 16.7 > 16.7 > 16.7
9A Cannatonic 0.0133 > 16.7 >16.7 > 16.7
10A Cannatonic 0.0107 > 16.7 > 16.7 > 16.7
11A Girl Scout Cookies 0.00582 > 16.7 0.190 > 16.7
12A Harle OG 0.0902 > 16.7 > 16.7 > 16.7
13A Harle Tsu 0.208 > 16.7 > 16.7 > 16.7
14A Harlequin 0.0245 > 16.7 > 16.7 > 16.7
14B Harlequin 0.0211 > 16.7 > 16.7 > 16.7
15A Infinite Euphoria 0.00725 > 16.7 0.160 > 16.7
 US 10,806,707 B2
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TABLE 8 - continued
GPCR Activation Activity of Cannabis Oils Determined
by Cell- Based Assays
CNR1 CNR2

CAMP EC50 B -Arrestin CAMP EC50 B - Arrestin

Example Strain (UM ) EC50 (UM ) (UM ) EC50 (UM )
16A Medihaze 0.0202 > 16.7 > 16.7 > 16.7
16B Medihaze 0.0248 > 16.7 > 16.7 > 16.7
17B Prize Kush 0.00405 > 16.7 0.340 > 16.7
18A Sour Kush 0.00374 > 16.7 0.185 > 16.7
19A Blueberry 0.00176 6.76 0.504 > 16.7

15
Example 6. Preparation of Blended Oil ACDC preparation and the Blueberry preparation . The can
Compositions nabinoid content and the terpene content of the blended oil
compositions is summarized in Table 9 , Table 10 , and Table
Cannabis extracts were prepared as described above, 11. The extracts were weighed in a sterile glass beaker and
using a C. sativa strain ( Infinite Euphoria ), C. indica strains 20 heated on a hot plate set to 70-110 ° C. in order to reduce
(Agoo , Girl Scout Cookies ) , ACDC , and Blueberry. Blended viscosity and facilitate mixing. The oil blends were stirred
oil compositions were prepared by combining the C. sativa between 5 minutes and 30 minutes with a glass stir rod until
preparation and / or the C. indica preparation ( s ) with the homogenous.
TABLE 9
Blended oil composition - cannabinoid and terpene content of component extracts
and blended oil composition .
C. indica C. sativa Blueberry ACDC Blended oil
preparation preparation preparation preparation composition
20-1 20-2 20-3 20-4 20
Component % ( w /w ) % ( w /w ) % ( w /w ) % (w / w ) % ( w /w )
THC 82.3 % 71.5 % 68.8 % 2.8 % 32.0 %
??? 1.1 % 0.5 % ND 68.7 % 41.0 %
CBG 2.5 % 4.9 % ND 2.5 % 2.7 %
CBN 0.3 % 0.5 % 0.5 % ND 0.5 %
THCA ND 0.3 % 3.7 % ND ND
CBDA ND ND ND 0.6 % 0.5 %
CBGA ND ND 0.4% ND 0.2 %
total THC 82.3 % 71.8 % 72.0 % 2.8 % 32.0 %
total CBD 1.1 % 0.5 % ND 69.2 % 41.5 %
total cannabinoids 86.2 % 0.0 % 73.4 % 74.6 % 77.0 %
B - caryophyllene 0.4 % 2.3 % 0.7 % ND 0.4 %
a -humulene 0.2% 0.8 % 0.4% ND 0.2 %
guaiol 0.2 % ND ND 0.2% 0.4%
linalool 0.2 % 0.7 % 0.2 % 0.6 % 0.1 %
caryophyllene oxide ND 0.4 % 0.2 % 0.3 % 0.1 %
a -terpineol ND ND 0.1 % ND ND
endo - fenchyl alcohol ND ND ND ND ND
a- bisabolol 0.1 % ND 0.2 % 0.3 % 0.5 %
a- eudesmol 0.1 % ND 0.2 % 0.2 % 0.3 %
B -eudesmol 0.1 % ND ND 0.2 % 0.2 %
y - eudesmol ND ND ND 0.2 % ND
isoborneol ND ND ND 0.5 % 0.1 %
unknown 0.8 % ND ND ND ND
monoterpenes
unknown 2.5 % ND 1.2 % 0.1 % 1.0 %
sesquiterpenes
total terpenes 4.6 % 4.2 % 4.0 % 2.6 % 3.4 %
Mass 16.46 g 6.79 g 6.65 g 35.64 g 65.64 g

TABLE 10
THC -rich blended oil composition -cannabinoid and terpene content of
component extracts and blended oil composition .
C. indica C. sativa Blueberry ACDC Blended oil
preparation preparation preparation preparation composition
30-1 30-2 30-3 30-4 30
Component % ( w /w ) % ( w /w ) % (w / w ) % (w /w ) % (w /w )
THC 82.3 % 71.5 % 68.8 % 2.3 % 61.1 %
CBD 1.1 % 0.5 % ND 58.6 % 7.3 %
 US 10,806,707 B2
87 88
TABLE 10 - continued
THC -rich blended oil composition -cannabinoid and terpene content of
component extracts and blended oil composition .
C. indica C. sativa Blueberry ACDC Blended oil
preparation preparation preparation preparation composition
30-1 30-2 30-3 30-4 30
Component % ( w /w ) % ( w /w ) % (w /w ) % ( w /w ) % ( w /w )
CBG 2.5 % 4.9% ND 1.9 % 1.9 %
CBN 0.3 % 0.5 % 0.5 % ND 0.8 %
THCA ND 0.3 % 3.7 % 0.9 % 0.6 %
CBDA ND ND ND 0.4% 0.2 %
CBGA ND ND 0.4 % 0.3 % 0.4 %
total THC 82.3 % 71.8 % 72.0 % 3.3 % 61.6 %
total CBD 1.1 % 0.5 % ND 59.0 % 7.5 %
total cannabinoids 86.2 % 0.0 % 73.4 % 0.0 % 72.2 %
B - caryophyllene 0.4 % 2.3 % 0.7 % 0.1 % 0.3 %
a -humulene 0.2 % 0.8 % 0.4 % 0.0 % 0.1 %
guaiol 0.2 % ND ND 0.0 % 0.2 %
linalool 0.2 % 0.7 % 0.2 % 0.1 % 0.1 %
caryophyllene ND 0.4 % 0.2 % ND ND
oxide
a - terpineol ND ND 0.1 % ND ND
endo - fenchyl ND ND ND ND ND
alcohol
a- bisabolol 0.1 % ND 0.2 % ND 0.2 %
a- eudesmol 0.1 % ND 0.2 % ND 0.2 %
B - eudesmol 0.1 % ND ND ND ND
y - eudesmol ND ND ND ND ND
isoborneol ND ND ND ND 0.1 %
unknown 0.8 % ND ND ND ND
monoterpenes
unknown 2.5 % ND 1.2 % ND 0.5 %
sesquiterpenes
total terpenes 4.6 % 4.2 % 4.0 % 0.4 % 1.8 %
Mass 52.13 g 8.58 g 8.58 g 15.92 g 85.21 g

TABLE 11 TABLE 11 - continued

35
CBD - rich blended oil composition - cannabinoid and terpene content of CBD -rich blended oil composition - cannabinoid and terpene content of
component extracts and blended oil composition . component extracts and blended oil composition .
C. indica Blueberry ACDC Blended oil C. indica Blueberry ACDC Blended oil
preparation preparation preparation preparation preparation preparation preparation preparation
40-1 40-2 40-3 40 40 40-1 40-2 40-3 40
Component % ( w /w ) % ( w /w ) % ( w /w ) % ( w /w ) Component % ( w /w ) % ( w /w ) % ( w /w ) % ( w /w )
THC 70.0 % 68.8 % 2.6 % 11.8 % total terpenes 3.9 % 4.0 % 4.3 % 3.5 %
CB 0.9 % ND 63.5 % 55.1 % Mass 4.96 g 12.61 g 107.92 g 125.49 g
CBG 5.1 % ND 2.0 % 2.7 %
CBN 0.3 % 0.5 % ND 0.3 %
THCA 1.9 % 3.7 % ND ND 45
CBDA ND ND 1.2 % 0.7 % Example 7. In Vitro Inhibition of Cancer Cell
CBGA 1.3 % 0.4 % ND 0.2 % Growth Using Blended Cannabis Oil Compositions
total THC 71.7 % 72.0 % 2.6 % 11.8 %
total CBD
total
0.9 %
79.6 %
ND
73.4 %
64.5 %
69.3 %
55.8 %
70.8 %
Brain cancer cell lines ( SF - 268 , SF - 295 , SF - 539 , SNB
cannabinoids 50 19 , SNB - 75 , U251 ) and breast cancer cell lines (MCF7 ,
B 1.7 % 0.7 % 0.4 % 0.5 % BT - 549, MDA - MB - 231 , MDA - MB - 468 , T -47D , HS 578T )
caryophyllene from the NC1-60 Human Tumor Cell Lines Screen were
a -humulene 0.9 % 0.4 % 0.2 % 0.2 % grown for three days in the presence of blended oil compo
guaiol ND ND 0.6 % 0.4 % sitions 20 , 30 , and 40 to determine the effects of the
linalool
caryophyllene
0.2 % 0.2 % 0.1 % ND compositions on cancer cell proliferation . Blended oil com
oxide
ND 0.2 % 0.1 % ND
55 positions were tested over a range of compositions , in
a - terpineol ND 0.1 % ND ND addition to single -strain composition 50 ( containing 64.1 %
endo- fenchyl ND ND ND ND CBD and 2.9 % THC ) , prepared from ACDC , and single
alcohol strain composition 60 ( containing 2.42 % CBD and 63.9 %
a- bisabolol 0.2 % 0.2 % 0.7 % 0.6 % THC ), prepared from Blueberry .
a- eudesmol ND 0.2 % ND 0.3 % 60 Percentage of growth inhibition was calculated using the
B - eudesmol ND ND
ND
ND
ND
0.2 % following parameters :
y - eudesmol ND ND
( Ti - Tz )/ ( C - Tz )x100 when Ti > Tz
isoborneol ND ND 0.1 % ND
unknown ND ND ND ND ( Ti - Tz ) > / Tzx100 when Ti < Tz
monoterpenes
unknown ND 1.2 % 2.1 % 1.2 % 65 Ti = absorbance after drug treatment
sesquiterpenes TzFabsorbance at time zero
C = absorbance of control growth .
 US 10,806,707 B2
89 90
Growth inhibition resulting from treatment with the 12. The composition of embodiment 11 , wherein
blended oil compositions and single - strain oil compositions at least 50 % of the total THC in the composition is present
in shown in FIGS . 4A , 5A , 6A , 7A , 8A , 9A , 10A , 11A , 12A , in the combined cannabinoid profile of the first can
13A , 14A , and 15A . Growth inhibition using mixtures of nabis oil preparation, the second cannabis oil prepara
A9 - THC and CBD having the same concentration of the oils 5 tion , and the third cannabis oil preparation, and
( THC : CBD Mix 20 , THC : CBD Mix 30 , THC : CBD Mix 40 , at least 50 % of the total CBD in the composition is present
THC : CBD Mix 50 , THC : CBD Mix 60 ) was also studied . in the fourth cannabinoid profile.
See , FIGS . 4B , 5B , 6B , 7B , 8B , 9B , 10B , 11B , 12B , 13B , 13. The composition of embodiment 12 , wherein
14B , and 15B . In addition , growth inhibition using isolated at least 75 % of the total THC in the composition is present
cannabinoids ( A8 THC , 19 THC , CBD , and 11Hy A9 THC ) 10 in the combined cannabinoid profile of the first can
was examined . See , FIGS . 4C , 5C , 6C , 7C , 8C , 9C , 10C , nabis oil preparation , the second cannabis oil prepara
11C , 12C , 13C , 14C , and 15C . Oils and compound mixtures tion , and the third cannabis oil preparation , and
were diluted in DMSO and added to cell culture in the at least 75 % of the total CBD in the composition is present
concentrations indicated in FIGS . 4-15 . At the highest 15 in the fourth cannabinoid profile.
concentration of oils tested , ( 30 uM) the percentage of 14. The composition of any one of embodiments 2-13 ,
growth inhibition ranged between -40 % and -80% . In the further comprising AP -tetrahydrocannabinolic acid
majority of experiments conducted, stronger cancer cell (THCA) , cannabidiolic acid (CBDA) , or a combination
growth inhibition was observed for cannabis oil composi thereof.
tions than isolated cannabinoids or mixtures of isolated 20 15. The composition of any one of embodiments 2-14 ,
cannabinoids. At the highest concentration of mixed can further comprising one or more cannabinoids selected
nabinoids tested , ( 30 uM) the percentage of growth inhibi from the group consisting of cannabigerol (CBG) ,
tion nged between 20 % and -50 % . The results indicate cannibinol ( CBN ) , and cannabigerolic acid (CBGA ).
that isolated cannabinoids do not provide the maximum 16. The composition of embodiment 1 , wherein the first
benefit associated with plant extracts containing an entou- 25 cannabis oil preparation is prepared from the AC/DC
rage of cannabinoids and terpenes. cannabis strain , and wherein the second cannabis oil
preparation is prepared from a Cannabis sativa strain .
VII . Exemplary Embodiments 17. The composition of embodiment 16 , wherein the ratio
of the first cannabis oil preparation to the second
Exemplary embodiments provided in accordance with the 30 cannabis oil preparation is about 1 : 1 by weight.
presently disclosed subject matter include , but are not lim 18. The composition of embodiment 1 , wherein the first
ited to , the claims and the following embodiments : cannabis oil preparation is prepared from the Medihaze
1. A composition comprising a first cannabis oil prepa cannabis strain , and wherein the second cannabis oil
ration having a first cannabinoid profile and a second 35 preparation is prepared from a Cannabis indica strain .
cannabis oil preparation having a second cannabinoid 19. The composition of embodiment 18 , wherein the ratio
profile . of the first cannabis oil preparation to the second
2. The composition of embodiment 1 , wherein the com cannabis oil preparation is about 1 : 1 by weight.
position comprises Aº -tetrahydrocannabinol ( THC ) 20. The composition of any one of embodiments 16-19 ,
and ( - ) -cannabidiol ( CBD ) . further comprising one or more essential oils selected
40 from the group consisting of bergamot essential oil ,
3. The composition of embodiment 1 or embodiment 2 , blood orange essential oil , neroli essential oil , lemon
wherein the ratio of THC to CBD ranges from about essential oil , peppermint essential oil , and spearmint
30 : 1 to about 1:30 weight. essential oil .
4. The composition of embodiment 3 , wherein the ratio of 21. The composition of any one of embodiments 16-19 ,
THC to CBD is about 4 : 1 by weight. 45 further comprising one or more essential oils selected
5. The composition of embodiment 3 , wherein the ratio of from the group consisting of Sweet Orange ( Citrus
THC to CBD is about 1 : 1 by weight. sinensis spp ) , Peppermint (Mentha piperita spp ) ,
6. The composition of embodiment 3 , wherein the ratio of Lemon ( Citrus limon spp ) , Lavender ( Lavendula
THC to CBD is about 1 : 2 by weight . angustifolia spp ) and Vanilla ( Vanilla planifolia spp ) .
7. The composition of embodiment 3 , wherein the ratio of 50 22. The composition of any one of embodiments 16-19 ,
THC to CBD is about 1:20 by weight. further comprising one or more essential oils selected
8. The composition of any one of embodiments 2-7 , from lavender essential oil and lemongrass essential
wherein greater than 50 % of the total THC in the oil .
composition is present in the first cannabinoid profile, 23. The composition of any one of embodiments 16-19 ,
and greater than 50 % of the total CBD in the compo- 55 further comprising vanilla essential oil .
sition is present in the second cannabinoid profile . 24. The composition of any one of embodiments 16-23 ,
9. The composition of embodiment 8 , wherein at least further comprising vitamin E.
75 % of the total THC in the composition is present in 25. The composition of embodiment 11 , wherein the first
the first cannabinoid profile, and at least 75 % of the cannabis oil preparation is prepared from a Cannabis
total CBD in the composition is present in the second 60 indica strain , the second cannabis oil preparation is
cannabinoid profile. prepared from a Cannabis sativa strain , the third can
10. The composition of any one of embodiments 2-7 , nabis oil preparation is prepared from the Blueberry
further comprising a third cannabis oil preparation cannabis strain , and the fourth cannabis oil preparation
having a third cannabinoid profile. is prepared from the AC /DC cannabis strain .
11. The composition of embodiment 10 , further compris- 65 26. The composition of embodiment 25 , where the ratio of
ing a fourth cannabis oil preparation having a fourth the first, second, third , and fourth cannabis oil prepa
cannabinoid profile. rations is about 55 : 10 : 10 : 25 by weight.
 US 10,806,707 B2
91 92
27. The composition of embodiment 25 or embodiment appreciate that certain changes and modifications may be
26 , further comprising vitamin E. practiced within the scope of the appended claims . In
28. A method for treating cancer, the method comprising addition , each reference provided herein is incorporated by
administering a composition according to any one reference in its entirety to the same extent as if each
claims 1-27 to a subject in need thereof. 5 reference was individually incorporated by reference.
A number of embodiments have been described . Never What is claimed is :
theless, it will be understood that various modifications may 1. A gel capsule consisting essentially of two or more of
be made without departing from the spirit and scope of the a cannabis
claimed invention . The flowcharts depicted in the figures do oil consistingsativa oil , a cannabis indica oil , and a cannabis
essentially of 50 % to 80% by weight canna
not require the particular order shown, or sequential order, to 10 bidiol and 1 % to 10 % by weight tetrahydrocannabinol; and
achieve desirable results . In addition, other steps may be
provided , or steps may be eliminated , from the described two or more of bergamot essential oil , blood orange essential
oil , neroli essential oil , lemon essential oil , peppermint
flows, and other components may be added to , or removed essential
from , the described composition . Accordingly, other oil , sweetoilorange , spearmint essential oil , lemongrass essential
embodiments are within the scope of the following claims . 15 vanilla essential oilessential
.
oil , lavender essential oil , and
It may be appreciated that the various method steps may 2. The gel capsule of claim 1 , wherein the gel capsule is
be performed in any order. The steps may also be merged formulated for oral administration.
with each other, may perform overlapping functions, or may
be coupled with other steps not shown to be connected in the formulated forcapsule
3. The gel of claim 1 , wherein the gel capsule is
pulmonary administration .
figures. Accordingly, the specification, the drawings, or a 20 4. A method for
combination thereof may be regarded in an illustrative rather thereof, the methodtreating Lyme disease in a human in need
consisting essentially of administering a
than a restrictive sense .
Although the foregoing invention has been described in therapeutically
to the human
effective amount of a gel capsule of claim 1
in need thereof.
some detail by way of illustration and example for purposes
of clarity of understanding, one of skill in the art will