Table S3.
Primers used for Realtime PCR and RT-PCR
Gene name Primers Product length
Mouse Epcam GAGTCCCTGTTCCATTCTT 250 bp
TCTCCTTTATCTCAGCCTTC
Mouse E-Cadherin GTCTCCTCATGGCTTTGC 228 bp
CTTTAGATGCCGCTTCAC
Mouse Twist1 AGCGGGTCATGGCTAACG 162 bp
GGACCTGGTACAGGAAGTCGA
mouse pri-miR-141 GGTCAGTTCCTTCAGCAGTG 185 bp
CCAAATGTGTTTCCAAATCT
mouse pri-miR-200a CCGAAGACAGGCTGATGATA 201
GTGACAATCCACTTCTACTC
mouse pri-miR-429 ACTGCCCTGCTGATGGATGT 170 bp
ACAGTTGTAACAGATGGATT
mouse pri-miR-200c TGATCTTGAAGGTGGACTGG 284 bp
CACTGGATTGGAGGAGGG
Mouse Zeb2 GAGCTTGACCACCGACTC 223 bp
TTGCAGGACTGCCTTGAT
mouse pri-miR-200b tgcagggctttctgctgttgtcc
cacccatatcgccaaggctaact
Mouse Foxc2 TCGACCCGGACTCCTACAA 255 bp
GGCTCAGCGTCTCCACCTT
mouse beta-actin CTGTCCCTGTATGCCTCTG 218 bp
ATGTCACGCACGATTTCC
Mouse DSG2 ACGGGCAGATAACAGACA 273 bp
GGGTCACAATCCCTTCAT
Mouse DSP GCAACAAGCCCATTATCC 203 bp
GCAAGAAAGGTCCACAGC
Mouse Pkp2 GCAGCCGAAGTAGGAAAC 216 bp
CCACCAATGTGGGTATCG
Mouse Cldn3 GGCGGCTCTGCTCACCTTA 116 bp
CGTACAACCCAGCTCCCATC
Mouse cldn4 GGGAATCTCCTTGGCAGTC 190
GGCGAGCATCGAGTCGTA
Mouse Grhl2 TTTGGTCCAACACCGTCTA 162 bp
CACTGGCAGCCCATACTT
Mouse GAPDH GTTGTCTCCTGCGACTTCA 184 bp
GGTGGTCCAGGGTTTCTTA
Mouse cldn7 GGGAGATGACAAAGCGAAGA 208 bp
CAGAAGGACCAGAGCAGACC
Mouse Gjb3 AGCATCGCCAGAAGCACG 278 bp
GGCAGAAGCGCCTACCAT
Mouse ocln ATCCACCTATCACTTCAGA 292 bp
TAATCTCCCACCATCCTC
Mouse Tjp2 AAGTTCCCTGCCTACGAG 189 bp
ATTCAACCGAACCACTCC
Mouse Wnt7A GGCAACCTGAGCGACTGT 457 bp or 302 bp
AGTAATTGGGTGACTTCTCG
Mouse Snai1 GTCGTCCTTCTCGTCCACC 391 bp
GGCCTGGCACTGGTATCTC
Mouse Snai2 TCCCATTAGTGACGAAGA 204 bp
CCCAGGCTCACATATTCC
Mouse Axin2 AGCCTAAAGGTCTTATGTGG 287 bp
ATGGAATCGTCGGTCAGT
Mouse Esrp1 TATTGAATCACCAGGGACG 269 bp or 257 bp
TTCGGTAGGAATCACAGC
Mouse CD44 GAAGGGCGAGTATAGAAC To detect
AGATGCCAAGATGATGAG alternative
splicing
Mouse CTNND1 GAACGAGGAAGAGGAAGA 612 bp, 494 bp,
GTACAGAAGGTGGTTGTGAT 205 bp
Mouse Enah AGGTGGAGGGAATACTGG 333 bp, 396 bp
GCACTTGGCTGTGATGAA
Mouse MAP3K7 CTGCCACAAACGACACTA 328 bp, 247 bp
GTCCCAGTAACAGTCAAGTC
Mouse ID2 GGTGGACGACCCGATGAGT 268 bp
TGCCTGCAAGGACAGGATG
Mouse Zeb1 CCATACGAATGCCCGAACT 386 bp
ACAACGGCTTGCACCACA
Mouse TCF4 CACAACGGAGCGATGGGTA 195 bp
GGGTGGGTTCAAGTCAGG
Human Wnt7A AGGGCAACCTGAGCGACTG 585 bp
GGGCGTACTGGTGGGTGTT
Human beta Actin CACCAACTGGGACGACAT 189 bp
ACAGCCTGGATAGCAACG
Human GAPDH GGATTTGGTCGTATTGGG 205 bp
GGAAGATGGTGATGGGATT
Human E-Cadherin CTGAGAACGAGGCTAACG 289 bp
TTCACATCCAGCACATCC
Human CD24 TACCCACGCAGATTTATT 162 bp
AGAGTGAGACCACGAAGA
Human Epcam ATCGTCAATGCCAGTGTA 288 bp
CTCGCTCAGAGCAGGTTA
Human Grhl2 CTTGACATTGCCGATTAC 224 bp
ATGGGTTTATTGCTACGATT
Human pri-miR-200a CTTACCGGACAGTGCTGGAT 72 bp
GCGGGTCACCTTTGAACATC
Human pri-miR-200b AGCTTCCCAGCGAGTCCCA 133 bp
TCCGCCGTCATCATTACCAG
Human pri-miR-200c GTGTTTGGGTGCGGTTGG 83 bp
AGGCGATGGATGTTGCTGAC
Human pri-miR-141 CCACCCAGTGCGATTTGT 234 bp
CGGGAGCCATCTTTACCAG
Human vimentin CCAGGCAAAGCAGGAGTC 213 bp
CGAAGGTGACGAGCCATT
Human Zeb1 GTGGCGGTAGATGGTAAT 254 bp
CTGTTTGTAGCGACTGGA
Human Zeb2 ACCAGCCCTTTAGGAGTT 272 bp
AGACCGACAGGCGGAATA
Human Sani1 CCCCAATCGGAAGCCTAA 261 bp
CCTTTCCCACTGTCCTCAT
Human Snai2 TCCTGGTCAAGAAGCATT 274 bp
GAGGAGGTGTCAGATGGA
Human Twist1 CGGGAGTCCGCAGTCTTA 161 bp
GCTTGAGGGTCTGAATCTTG
