PERHITUNGAN

1. Kurva Standar Xilosa

KURVA STANDAR XILOSA

0.9
0.8
f(x) = 0.13 x − 0.02
0.7 R² = 1
0.6
Absorbansi

0.5
0.4 Linear ()
0.3
0.2
0.1
0
1 2 3 4 5 6 7
Kadar Xilosa (μmol)

2. Kurva Standar BSA

KURVA STANDAR BSA

2
1.8
1.6 f(x) = 0.04 x + 0.34
R² = 1
1.4
Absorbansi

1.2
1
0.8 Linear ()
0.6
0.4
0.2
0
0 5 10 15 20 25 30 35 40 45
Kadar BSA (μg.ml-1)

A. Ekstrak Kasar
1. Aktivitas Enzim (U/mL)
V total = 800 µl
V enzim = 100 µl
FP =1
 t = 60 menit
- Kadar Xilosa Kontrol
Absorbansi Kontrol (Ak) = 0.352
y = 0.1255x - 0.02
0.352 = 0.1255x - 0.02
0.372 = 0.1255x
x = 2.96 μmol
x = [k] = 2.96 μmol
- Kadar Xilosa Sampel
Absorbansi Sampel (As) = 0.368
y = 0.1255x - 0.02
0.368 = 0.1255x - 0.02
0.388 = 0.1255x
x = 3.09 μmol
x = [S] = 3.09 μmol
- Aktivitas Total Enzim

[ S ] −[ k ] x fp x( Vtotal )
Aktivitas total = Venzim
t

[ 3.09 ] −[ 2.96 ] μmol x 1 x ( 800 μ L )

= 100 μ L
60 menit
0.13 μmol x 1 x 8
=
60 menit
1.04 μ mol
= = 0.01733 U
60 menit
2. Kadar Protein
Pers. Garis → y = 0.0368x + 0.337
Gradien (m) = 0.0368
Intersep (C) = 0.337
FP =1
Absorbansi = 0.514
 |±|C xfp
- Kadar Protein =
m
0.514 ± 0.337 x 1
=
0.0368
= 4.809 µg/mL
= 0.002809 mg/mL

B. Fraksinasi pada Fraksi 10

1. Aktivitas Enzim (U/mL)
V total = 800 µl
V enzim = 100 µl
FP =1
t = 60 menit
- Kadar Xilosa Kontrol
Absorbansi Kontrol (Ak) = 0.037
y = 0.1255x - 0.02
0.037 = 0.1255x - 0.02
0.057 = 0.1255x
x = 0.454 μmol
x = [k] = 0.454 μmol
- Kadar Xilosa Sampel
Absorbansi Sampel (As) = 0.103
y = 0.1255x - 0.02
0.103 = 0.1255x - 0.02
0.123 = 0.1255x
x = 0.980 μmol
x = [S] = 0.980 μmol
- Aktivitas Total Enzim

[ S ] −[ k ] x fp x( Vtotal )
Aktivitas total = Venzim
t
 [ 0.980 ] −[ 0.454 ] μmol x 1 x ( 800 μ L )
= 100 μ L
60 menit
0.526 μmol x 1 x 8
=
60 menit
4.208 μ mol
= = 0.0701333 U
60 menit
2. Kadar Protein
Pers. Garis → y = 0.0368x + 0.337
Gradien (m) = 0.0368
Intersep (C) = 0.337
FP =1
Absorbansi = 0.567
|±|C xfp
- Kadar Protein =
m
0.567 ±0.337 x 1
=
0.0368
= 6.25 µg/mL
= 0.00625 mg/mL

C. Dialisat Enzim
1. Aktivitas Enzim (U/mL)
V total = 800 µl
V enzim = 100 µl
FP =1
t = 60 menit
- Kadar Xilosa Kontrol
Absorbansi Kontrol (Ak) = 0.356
y = 0.1255x - 0.02
0.356 = 0.1255x - 0.02
0.376 = 0.1255x
x = 2.996 μmol
x = [k] = 2.996 μmol
 - Kadar Xilosa Sampel
Absorbansi Sampel (As) = 0.389
y = 0.1255x - 0.02
0.389 = 0.1255x - 0.02
0.409 = 0.1255x
x = 3.259 μmol
x = [S] = 3.259 μmol
- Aktivitas Total Enzim

[ S ] −[ k ] x fp x( Vtotal )
Aktivitas total = Venzim
t

[ 3.259 ] −[ 2.996 ] μmol x 1 x ( 800 μ L )

= 100 μ L
60 menit
0.263 μmol x 1 x 8
=
60 menit
2.104 μ mol
= = 0.035067 U
60 menit
2. Kadar Protein
Pers. Garis → y = 0.0368x + 0.337
Gradien (m) = 0.0368
Intersep (C) = 0.337
FP =1
Absorbansi = 0.514
|±|C xfp
- Kadar Protein =
m
0.514 ± 0.337 x 1
=
0.0368
= 4.81 µg/mL
= 0.00481 mg/mL