MODULE
5 CHEM 403L CHEMICAL BIOLOGY III – BIOMOLECULES
Qualitative Analysis of Carbohydrates
At the end of the module, the students must be able to:
1. describe the molecular structure of sugars and polysaccharides;
2. understand digestion and appreciate the complexity of the human body;
3. experiment with different foods and measure their impact on the blood sugar level;
4. identify the different color reaction tests necessary to characterize the
structural composition of the carbohydrates;
5. provide the underlying principle involved in each qualitative test of carbohydrates;
and
6. perform a Benedict test on four unknown solutions to test for the presence and
relative concentration of simple sugars.
Virtual laboratory activity/simulations/videos/readings:
Virtual Simulations 1. Labster/Carbohydrates: the sugar that feed us
2. OLabs/Qualitative Analysis of Carbohydrates (http://amrita.olabs.edu.in/?
sub=73&brch=8&sim=209&cnt=4)
3. McGraw Hill Virtual labs/ Chemical composition of cells • test for sugars
Videos General tests for Carbohydrates
1. Molisch’s Test (https://youtu.be/etE8LCWvb-U)
2. Anthrone test (https://youtu.be/SYe8Z0oP9Qk)
3. Iodine test (https://youtu.be/EBbiQw7Jolk)
Tests for reducing sugars.
4. Benedict’s Test (https://youtu.be/TDFbtEwbmz0)
5. Fehling’s Test (https://youtu.be/DMDB-pS6tks)
Specific tests for Carbohydrates
6. Seliwanoff’s Test (https://youtu.be/88hWF1-RrTQ)
7. Bial’s Orcinol Test (https://youtu.be/JboA8Ghyz5A)
8. Barfoed’s Test (https://youtu.be/yQfMqvOxPrc)
9. Ozasone Test (https://youtu.be/R2Jhyp7ZU5s)
Overall video
10. Qualitative Analysis of Carbohydrates - MeitY OLabs
(https://www.youtube.com/watch?v=ojhdTFmkY1c&t=322s)
Module 5: For teaching purposes only
Prepared and complied by: Mr. Aldrin P. Bonto/ Department of Chemistry, College of Science, UST
Post-laboratory questions
1. What are the principles behind the following qualitative tests?
a. Molisch’s test
b. Anthrone test
c. Iodine test
d. Benedict’s test
e. Fehling’s test
f. Seliwanoff’s test
g. Bial’s test
h. Barfoed’s test
i. Ozasone test
2. Please complete the following tables.
Table 1.A. General tests for Carbohydrates
Chemical test Reagents used Positive visible results Principle of detection
Molisch’s test
Anthrone test
Iodine test
Table 1.B. General tests for Carbohydrates
Molisch’s Test Anthrone Test Iodine Test
Color of the Reagent
Amylose
Glycogen
Cellulose
Table 2.A. Comparative Tests for Reducing Sugars
Chemical test Reagents used Positive visible results Principle of detection
Benedict’s Test
Fehling’s test
Table 2.B. Comparative Tests for Reducing Sugars
Chemical structure Benedict’s Test Fehling’s test
Color of Reagent N/A
Sucrose
Maltose
Lactose
Cellobiose
Glucose
Galactose
Mannose
Fructose
Ribose
Xylose
Module 5: For teaching purposes only
Prepared and complied by: Mr. Aldrin P. Bonto/ Department of Chemistry, College of Science, UST
Table 3.A. Specific tests for Carbohydrates
Chemical test Reagents used Carbohydrate Positive visible Principle of detection
identified results
Seliwanoff’s Test
Barfoed’s Test
Bial’s Test
Mucic Acid Test
Table 3.B. Specific tests for Carbohydrates
Seliwanoff’s Test Barfoed’s Test Bial’s Test Ozasone Test
Color of Reagent
Sucrose
Maltose
Lactose
Cellobiose
Glucose
Galactose
Mannose
Fructose
Ribose
Xylose
Module 5: For teaching purposes only
Prepared and complied by: Mr. Aldrin P. Bonto/ Department of Chemistry, College of Science, UST
