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FC 106 Experiment 20 CRIM3 2B

The document describes preliminary tests that can be used to detect potential poisons in specimens. It lists microscopic examination of specimens to identify foreign substances and note color changes. Specific colors could indicate certain poisons such as black for phenols or red for carbon monoxide. Flame tests are also described to detect elements like barium, copper, or lithium based on flame color. While preliminary tests can indicate potential poisons, they are not conclusive and further analysis is required to identify specific toxic compounds. Proper collection and preservation of specimens like blood, urine, and organs is necessary for accurate analysis.

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340 views3 pages

FC 106 Experiment 20 CRIM3 2B

The document describes preliminary tests that can be used to detect potential poisons in specimens. It lists microscopic examination of specimens to identify foreign substances and note color changes. Specific colors could indicate certain poisons such as black for phenols or red for carbon monoxide. Flame tests are also described to detect elements like barium, copper, or lithium based on flame color. While preliminary tests can indicate potential poisons, they are not conclusive and further analysis is required to identify specific toxic compounds. Proper collection and preservation of specimens like blood, urine, and organs is necessary for accurate analysis.

Uploaded by

Earl John
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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College of Criminology

University of Luzon

Experiment No. 20
Preliminary Test for Poison

OBJECTIVE: To know the different preliminary test which must be done to in a systematic
toxicological analysis of the specimen.

MATERIALS: Internal organs, glass tubing, evaporating dish, nichrome wire and hand lens.

PROCEDURE:

MICROSCOPIC EXAMINATION: Place the specimen-submitted on an evaporating dish and


take note of the presence of any foreign substance, solid, or liquid. Note the
color and examine if the material is uniformly colored or its different parts
distinguishable by difference in color in the light of the following possibilities.

BLACK COLORATION ………….( O – phenol, M – phenol, P – phenol )


RED COLORATION ……………. ( Carboxyhemoglobin poisoning )
BROWNISH COLORATION …. ( Homicide poisoning, Ex. Aluminum phosphide )
YELLOWISH COLORATION … ( Chromite compounds and sulfur poisoning )
GREEN COLORATION …. ( Cyanobacteria poisoning, Ex. Anatoxin, guanitoxin )
VIOLET COLORATION …..( Hypostasis poisoning, Ex. Carbon monoxide and
cyanide poisoning )
BLUE COLORATION ………… ( Nitrate and nitrite poisoning )

If crystalline substances are present whether found in the stomach content or as a pure
substance, take notes of its behavior toward heat and its melting point. The color in the flame
test should also be taken into consideration. Reaction with litmus paper must be noted. Acidic
reaction is suggestive of the presence of a free acids, acid salts or readily hydrolyzed salts.
Alcoholic reaction indicates the presence of caustic alkali or ammonia.

OBSERVATION: Give the result of your observation obtained under the different preliminary
test. Tabulate below:

BLUISH FLAME INDICATES.. ( Cesium, Arsenic, Selenium, Mercury, Antimony )


BLUISH GREEN INDICATES …………... ( Barium and Copper )
BROWN INDICATES …………………….. ( Rubidium )
CARMINE RED INDICATES ……………. ( lithium and strontium )
YELLOW RED INDICATES ……………... ( Calcium )
VIOLET INDICATES ……………………… ( Potassium )
YELLOW INDICATES ……………………. ( Sodium and Iron )
College of Criminology
University of Luzon

CONCLUSION:

1. What is the importance of making the preliminary test?

It is used to determine the presence of poisons and other potentially toxic agents in blood,
urine, or other bodily substances.

2. Are preliminary tests conclusive in the presence or absence of poison? Explain.

No, because there is no specific substance that causes poison. On the other hand compounds
containing toxic, and it is not conclusive in presence.

3. How would you collect and preserve the specimen? Explain.

Urine and blood 30ml put preservative with sodium chloride, 30 grams to preservative kidney.
Stomach contained lungs, liver to collect 30 grams and put in the refrigerator with controlled
temperature.
College of Criminology
University of Luzon

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