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CAPE Lab #1 - Oxygen Uptake With Respirometer. HHS. T. Smith

1) The document describes a lab experiment to investigate the rate of oxygen uptake of germinating seeds using a simple respirometer. The apparatus includes soda lime stones, germinating bean seeds, a wire gauze, dye, a capillary tube, and a ruler. 2) The method involves placing soda lime stones and seeds in a syringe barrel, attaching a capillary tube filled with dye, and measuring the distance the dye moves over time, which indicates oxygen uptake. 3) Results would be recorded in a table, and a graph of distance vs. time would be plotted and the gradient calculated. The control experiment repeats the method without soda lime. Calculations, conclusions, and

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Rocheal Whittingham
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917 views4 pages

CAPE Lab #1 - Oxygen Uptake With Respirometer. HHS. T. Smith

1) The document describes a lab experiment to investigate the rate of oxygen uptake of germinating seeds using a simple respirometer. The apparatus includes soda lime stones, germinating bean seeds, a wire gauze, dye, a capillary tube, and a ruler. 2) The method involves placing soda lime stones and seeds in a syringe barrel, attaching a capillary tube filled with dye, and measuring the distance the dye moves over time, which indicates oxygen uptake. 3) Results would be recorded in a table, and a graph of distance vs. time would be plotted and the gradient calculated. The control experiment repeats the method without soda lime. Calculations, conclusions, and

Uploaded by

Rocheal Whittingham
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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LAB #

SKILL A/I/ MM/ ORR

Title: Respiration and oxygen uptake

Aim: To investigate the rate of oxygen uptake of germinating seeds using a simple
respirometer

Apparatus/Materials: soda lime stones, dye, germinating bean seeds, wire gauze, dye,
capillary tube, ruler

Method:

1. Place a few soda lime stones into the barrel of a syringe and cover with a disc of wire
gauze. Label the syringe A. Soda lime absorbs carbon dioxide gas.
2. Add 3-4 germinating seeds into the barrel of the syringe. Insert the plunger of the
syringe and allow the plunger to just touch the seeds. Note that the seeds were surface
sterilized before germination.
3. Attach 1mm diameter capillary tube to the end of the syringe
4. Carefully dip the end of the capillary tube into the dye
5. Slowly and carefully pull up the drop of dye a short distance into the capillary tube by
releasing the syringe plunger
6. Place a ruler alongside the capillary tube to measure the distance moved by the dye
during the experiment.
7. Record the starting position of the dye as the initial position of the dye
8. Record the distance the dye moves at two-minute intervals for 10 minutes
9. Repeat the experiment
10. Calculate the average movement of the dye by combining the results from the two
experiments
11. Set up a control experiment. Set up the experiment in a syringe labelled B without
adding soda lime stones.
12. Record all results in a suitable table
13. Plot a graph using the results, distance in mm vs time in seconds. Calculate the
gradient of the graph.

Results: Written in an appropriate table with descriptive title in capital letters and underline.

Discussion
1. Without coating any numerical values explain how the
respirometer measures oxygen uptake. 1
2. Explain the importance of removing the testa before
placing it inside the plunger. 1

3. Calculate the volume of O;

- use of formula 1

- accurate data calculated to 1hr. 1

- correct answer with appr. units 1

4. Correct assumptions of data used in calculation 1

5. correct conclusion about the validity of assumption 1

Background Information

Explain what is respiration, the types that exist and the relevance of each 2

Explanation of the results 2

Suitable inferences made about the role of the soda lime base on the 2
control

Limitations:

Explain what conditions could not be controlled and therefore qualify as a limitation. 2

Total: 15 Scale to 12 marks

 Skill (ORR)

Lab sequence (1 mark)

Method (2 marks)

--Past tense -1
--Grammar -1

Results Table (3 marks)

--Appropriate title (1 mark)

--Layout of results (1 mark)

--Units (1 mark)

Graph( 6 marks)

--Title (1 mark)

--Scale (1 mark)

--Appropriate choice of Axes (1 mark)

Along with units (1 mark)

Correct plotting of points (2 marks)

MM

Criteria  Maximum Marks Teacher’s  Moderator’s


Allotted  Score 
Score 

Apparatus /utensils used were clean 1

Correct Reagents used in the experiment.  1

Total amount of KOH was approximately to 1


2ml. 

Recording accurate date from the meniscus of 1


measuring cylinder

Reading measuring cylinder  at eye level.  1

Pouring of KOH without spill 1

Carefully placing the germinating seeds inside 1


of the respirometer

Carefully attaching the capillary tube to the 1


syringe

Dipping the capillary tube in dye without 1


spilling  

Pulling down of dye to a suitable level in the 1


capillary tube

Staging the ruler without disturbing the dye  1

Reading of ruler or capillary tube to record 1


initial and final position of the dye. 

TOTAL  12 Marks 

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