Measure

what is measurable
and make measurable
that which is not.
Galileo Galilei (1564-1642)

Instruction Manual and Safety Information

Abbemat 3000
Abbemat 3100
Abbemat 3200
Automatic Refractometer

Instrument Software Version: 1.00

(Original Instructions)
Instruction Manual and Safety Information
Abbemat 3000
Abbemat 3100
Abbemat 3200
Automatic Refractometer

Instrument Software Version: 1.00

(Original Instructions)
Disclaimer
This document may contain errors and omissions. If you discover any such errors or if you would like to see
more information in this document, please contact us at our address below. Anton Paar assumes no liability
for any errors or omissions in this document.

Changes, copyright, trademarks etc.

This document and its contents may be changed or amended by Anton Paar at any time without prior notice.
All rights reserved (including translation). This document, or any part of it, may not be reproduced, changed,
copied, or distributed by means of electronic systems in any form (print, photocopy, microfilm or any other
process) without prior written permission by Anton Paar GmbH.
Trademarks, registered trademarks, trade names, etc. may be used in this document without being marked
as such. They are the property of their respective owner.

Further information
Published by Anton Paar OptoTec GmbH, Germany
Printed by Anton Paar OptoTec GmbH, Germany
Copyright © 2018 Anton Paar GmbH, Graz, Austria
Address of the instrument producer: Anton Paar OptoTec GmbH
Lise-Meitner-Str. 6
30926 Seelze-Letter / Germany – Europe

Tel: +49 (0) 511 400950

Fax: +49 (0) 511 4009534
E-Mail: [email protected]
Web: www.anton-paar.com

Date: 2019-12-19

Document number: H79IB001EN-D

 Contents

Contents
1 Safety Instructions.................................................................................................................................. 7
1.1 General Safety Instructions............................................................................................................... 7
1.2 Conventions for safety messages..................................................................................................... 8
1.3 Safety Signs on the Instrument......................................................................................................... 8
2 An Overview ............................................................................................................................................ 9
3 Checking the Supplied Parts ............................................................................................................... 10
4 Functional Components....................................................................................................................... 11
4.1 Front View....................................................................................................................................... 11
4.2 Rear View ....................................................................................................................................... 11
5 Preparing the Instrument ..................................................................................................................... 12
5.1 Switching the Instrument On/Off ................................................................................................... 12
6 Putting Abbemat into Operation.......................................................................................................... 13
7 Installing Optional Input/Output Devices ........................................................................................... 15
7.1 External Keyboard, Bar Code Reader, Mouse................................................................................ 15
7.2 USB Memory Device....................................................................................................................... 15
7.3 Printer ............................................................................................................................................. 15
7.3.1 Connecting a Printer ............................................................................................................... 15
7.3.2 Installing, Editing, Deleting a Printer....................................................................................... 15
7.3.3 Setting Automatic Export ........................................................................................................ 16
7.3.4 Defining the Name and Address of the Report ....................................................................... 16
7.3.5 Defining the Logos of the Report ............................................................................................ 16
8 General Settings ................................................................................................................................... 17
8.1 Date and Time ................................................................................................................................ 17
8.2 Regional Settings............................................................................................................................ 17
8.3 Network........................................................................................................................................... 17
8.4 Instrument Name and Location....................................................................................................... 17
8.5 LIMS Settings.................................................................................................................................. 17
8.6 Unit Settings.................................................................................................................................... 17
8.7 Display Brightness .......................................................................................................................... 17
8.8 Screen Saver .................................................................................................................................. 17
9 Checking, Adjusting and Calibrating .................................................................................................. 18
9.1 Definitions ....................................................................................................................................... 18
9.2 Refractive Index Checks ................................................................................................................. 18
9.3 Adjustments .................................................................................................................................... 19
9.3.1 Setting the Water Reference .................................................................................................. 19
9.3.2 One Point Adjustments ........................................................................................................... 19
9.3.3 Calibrating the Sample Illumination ........................................................................................ 19
9.3.4 Reset to Factory Adjustment .................................................................................................. 19
10 Methods ............................................................................................................................................... 20
10.1 Changing Method Parameters ...................................................................................................... 20
10.2 Installing a Method Package......................................................................................................... 20
11 Measuring ............................................................................................................................................ 21
11.1 Automatic Temperature Control.................................................................................................... 21
11.2 Selecting a Method ....................................................................................................................... 21
11.3 Applying a Sample ...................................................................................................................... 21
11.4 Magnetic Sample Cover................................................................................................................ 21
11.5 Performing Measurements............................................................................................................ 22
11.6 Measurement Accuracy ................................................................................................................ 22

H79IB001EN-D 5
 Contents

12 Handling the Measurement Data ....................................................................................................... 23

12.1 Data Memory ................................................................................................................................ 23
12.2 Viewing Results ............................................................................................................................ 23
12.3 Print and Export Options............................................................................................................... 23
12.4 Printing or Exporting Data............................................................................................................. 23
12.5 Deleting Data ................................................................................................................................ 23
13 Upkeep and Cleaning ......................................................................................................................... 24
13.1 Cleaning Instrument Housing and Touchscreen........................................................................... 24
13.2 Cleaning the Measuring Prism...................................................................................................... 24
13.3 Drying Cartridge............................................................................................................................ 24
13.4 Storing the Instrument................................................................................................................... 25
14 Maintenance and Repair..................................................................................................................... 26
14.1 Warranty ....................................................................................................................................... 26
14.2 Repair Performed by an Authorized Anton Paar Representative ................................................. 26
15 Updates and System Information..................................................................................................... 27
15.1 Updating the Instrument Software ................................................................................................ 27
15.2 System Information ....................................................................................................................... 27
15.3 Saving an Instrument Log File ...................................................................................................... 27
15.4 Live Raw Data............................................................................................................................... 27
Appendix A: Technical Data ................................................................................................................... 28
Appendix A.1: General Technical Data ................................................................................................ 28
Appendix A.2: Wetted Parts.................................................................................................................. 28
Appendix A.3: Specifications ............................................................................................................... 29
Appendix A.4: Menu Tree .................................................................................................................. 29
Appendix B: Troubleshooting ................................................................................................................ 30
Appendix C: CE Certificate ..................................................................................................................... 31

6 H79IB001EN-D
 1 Safety Instructions

1 Safety Instructions
• Read the documentation before using the instru- • Make sure all operators are trained to use the
ment. instrument safely and correctly before starting
any applicable operations.
• Follow all hints and instructions in the documen-
tation to ensure the correct use and safe func- • In case of damage or malfunction, do not con-
tioning of the instrument. tinue operating the instrument. Do not operate
the instrument under conditions which could
• The documentation is a part of the product.
result in damage to goods or injuries or loss of
Keep it for the complete working life of the prod-
life.
uct and make sure it is easily accessible for all
persons involved with the product. If you receive • If liquid has been spilled over the instrument,
any additions or revisions to the documentation disconnect the instrument from the AC power
from Anton Paar GmbH, these must be treated supply. Clean and dry the housing of the instru-
as part of the documentation. ment. If you have a suspicion that liquid got into
the instrument, have the instrument cleaned and
checked for electrical safety by a service techni-
1.1 General Safety Instructions cian.

Liability Maintenance and service

• This document does not claim to address all • Service and repair procedures may only be car-
safety issues associated with the use of the ried out by authorized personnel or by Anton
instrument and samples. It is your responsibility Paar GmbH.
to establish health and safety practices and to
determine the applicability of regulatory limita- Disposal
tions. • Concerning the disposal of the instrument,
• Anton Paar GmbH warrants the proper function- observe the legal requirements in your country.
ing of the instrument only if no modifications
have been made to mechanics, electronics, Returns
firmware or software. • For repairs send the cleaned instrument to your
Anton Paar representative. Only return the
• Only use the instrument for the purpose instrument together with the filled out RMA
described in the documentation. Anton Paar (Return Material Authorization) and the form
GmbH is not liable for damages caused by the "Safety Declaration for Instrument Repairs".
incorrect use of the instrument. Please download the Safety Declaration form
• The results delivered by the instrument depend from our website www.anton-paar.com.
not only on the correct functioning of the instru- • Do not return instruments which are contami-
ment, but also on various other factors. We nated by radioactive materials, infectious agents
therefore recommend you have the results or other harmful substances that cause health
checked (e.g. plausibility tested) by skilled per- hazards.
sonnel before consequential actions are taken
based on the results. General precautions
Installation and use • Observe and adhere to your national safety reg-
ulations regarding the handling of all substances
• The instrument is not explosion-proof and associated with your measurements (e.g. use
therefore must not be operated in areas with risk safety goggles, gloves, respiratory protection,
of explosion. etc.).
• The installation procedure should only be car- • Before a measurement, check the wetted parts
ried out only by authorized personnel who are of the instrument for chemical resistance to the
familiar with the installation instructions. samples and cleaning agents used.
• Do not use any accessories or spare parts other • Take care that the liquids (samples and cleaning
than those supplied or approved by Anton Paar agents) or gases that you use are chemically
GmbH. compatible when they come into contact with

H79IB001EN-D 7
1 Safety Instructions

each other. They shall not react exothermically 1.2 Conventions for safety messages
or produce solid particles which might stick to
the sample interface.
The following conventions for safety messages are
• Before you start a measurement or cleaning used in this instruction manual:
procedure, take care that all parts, especially
the measuring cells, the hoses, and the waste
vessel, are properly connected and in good con-
dition. WARNING
• Take measures that spilled liquids cannot get Description of risk.
into plug connections or venting slots of electri- Warning indicates a hazardous situation which, if
cal appliances. not avoided, could result in death or serious
injury.
• Connect the measuring system to the AC power
supply via a safety switch located at a safe dis-
tance from the instruments. In an emergency,
turn off the power using this switch instead of
CAUTION
the power switch on the instruments. Description of risk.
• Ensure the sufficient supervision of the instru- Caution indicates a hazardous situation which, if
ment during operation. not avoided, could result in minor or moderate
injury.
Precautions for highly inflammable samples
and cleaning agents
NOTICE
• Keep potential sources of ignition, like sparks or Description of risk.
open flames, at a safe distance from the instru- Notice indicates a situation which, if not avoided,
ment. could result in damage to property.
• Place the instrument on a laboratory bench
made of fireproof material, preferably bricks,
ceramics, or stoneware. 1.3 Safety Signs on the Instrument
• Store only the minimum required amount of
sample, cleaning agents and other inflammable
materials near the instrument.
CAUTION
• Do not spill sample/cleaning agents or leave
their containers uncovered. Immediately remove Hot surface
spilled sample/cleaning agents. The measuring prism, its surrounding steel sur-
face or mounted accessories can be very hot.
• Make sure that the setup location is sufficiently Do not touch these surfaces without adequate
ventilated. The environment of the instrument protective measures.
must be kept free of inflammable gases and
vapors.
• Provide a fire extinguisher.

8 H79IB001EN-D
 2 An Overview

2 An Overview

The Abbemat 3000/3100/3200 is an automatic re- The refractive index of a dissolved substance in a
fractometer for measuring the refractive index of liq- solvent correlates with its concentration and can
uid or paste-like samples at high accuracy. therefore be used for concentration measurements.
Abbemat 3000/3100/3200 refractometer family was Many other scales can be derived from the refrac-
developed to satisfy quality control or research re- tive index, e.g. the freezing point of cooling water.
quirements with precision and ease of use.
Refractive index data is usually related to a standard
The refractive index of a pure material is a physical temperature, e.g. 20 °C or 25 °C, because the tem-
property and can be used to identify and character- perature dependence of the refractive index is spe-
ize materials. cific to each sample. Therefore, an accurate
temperature measurement of the refractometer
prism and sample is essential.

H79IB001EN-D 9
3 Checking the Supplied Parts

3 Checking the Supplied Parts

Your Abbemat was tested and packed carefully be- 1. Keep the packaging material (box, foam piece,
fore shipment. However, damage may occur during transport protection) for possible returns.
transport.
2. Check the delivery note and compare it with the
TIP: An instrument stored at low temperatures can supplied parts.
cause condensation of moisture on sensitive parts.
3. If a part is missing, contact your Anton Paar rep-
To prevent malfunctions allow the transport pack-
resentative immediately.
age and its contents to come to thermal equilibrium
prior to opening. Therefore the unopened package 4. If a part is damaged, contact the transport com-
should first be stored in a dry room at normal tem- pany and your Anton Paar representative.
perature for a few hours.
Table 3-1: Supplied Parts

Symbol Pcs. Article Description Mat. No.

1 Abbemat 3000 173451

Abbemat 3100 173452
Abbemat 3200 173453

1 Instruction manual (English) 179373

1 External Power Supply 99780

1 Power cord USA 52656

Power cord UK 61865
Power cord Europe 65146

1 Magnetic sample cover 101659

1 Drying cartridge 107786

5 Pasteur pipette (3 mL) 47447

10 H79IB001EN-D
 4 Functional Components

4 Functional Components
4.1 Front View
1

Fig. 4-1: Front view of the Abbemat 3200

1 Drip plate
2 Magnetic sample cover (covering the sample well and the prism)
3 LCD color screen

4.2 Rear View

1 2 3 4 5 6 7 8

Fig. 4-2: Rear view of the Abbemat 3200

1 Drying Cartridge (Abbemat 3100/3200) 5 RS-232 interface

2 Ventilation openings 6 Power inlet
3 Ethernet LAN interface 7 Power switch
4 USB port for service purposes 8 Two USB ports

H79IB001EN-D 11
5 Preparing the Instrument

5 Preparing the Instrument

The Abbemat 3000/3100/3200 is designed for oper-
ation under typical laboratory conditions. To reach
5.1 Switching the Instrument On/Off
the highest possible precision for your measure-
ments, do not place the instrument:
• near a heater WARNING
• near an air conditioner • Before switching on the instrument, make
sure that the line voltage is 100 V AC to
• on a vibrating surface or close to vibrating 240 V AC, 50/60 Hz. If large voltage fluctua-
equipment tions are to be expected, a constant voltage
• in direct sunlight source (UPS) is recommended.
• The non-fused earth conductor of the power
cord (or power inlet) has to be connected to
WARNING earth.

• Ensure that the power plug is always easily

accessible so that the instrument can easily 1. Connect the power inlet of the Abbemat 3000/
be disconnected from the mains at any time. 3100/3200 with the plug of the external power
supply.
• Ensure that the ventilation through the rear
and bottom of the instrument is not blocked 2. Connect the external power supply to the mains
by any obstacles. using the power cord.
To switch the instrument on or off, use the power
switch at the rear of the instrument.
After turning on the power, the instrument needs ap-
proximately 15 minutes for temperature equilibra-
tion and internal temperature adjustments.

12 H79IB001EN-D
 6 Putting Abbemat into Operation

6 Putting Abbemat into Operation

Header Time and
date

Quick
Output access
fields area

Temperature
Input control
fields

Progress
bar
Buttons
area

Fig. 6-1: Main screen example (Abbemat 3200) can be used to enter a sample name or a user-de-
fined data field to assign additional information to
To operate the Abbemat you can use the touch-
your samples, see section 10.1.
screen, a computer mouse, an external keyboard or
a bar code reader. Abbemat 3100 and Abbemat 3200 provide a fast
and precise automatic temperature control of the
Using the Touchscreen sample, see section 11.1.

NOTICE A progress bar indicates if the instrument is cur-

rently measuring. It turns green when a measure-
• Only use clean and dry fingers to operate the ment is finished.
touchscreen.
Buttons Area
• Never use any sharp objects.
Use the buttons on the screen by directly tapping on
• Operate the touchscreen with gentle fingertip
them. If an external keyboard is connected, you can
pressure only.
also use the function keys <F1> to <F5> to operate
To select an item in a table or list, directly tap on it. the five buttons within the buttons area: <F1> corre-
If you hold your finger on a data item for a prolonged sponds to the leftmost button, <F5> corresponds to
time, a context menu will pop up. Just tap outside the rightmost button.
the menu once to exit the context menu:
<F1> or To open the main menu.
<Menu>

<F2> or To view the measured data.

<Meas. Data>

<F3> or Choose and configure the

<Method> measurement method Refractive
Index, Brix, Fructose, Glucose,
Fig. 6-2: A long tap evokes a context menu Invert Sugar or Sucrose

<F4> or To export or print the measured

Operating elements on the main screen
<Export> data.
The left part of the header displays the name of the
instrument. While navigating in menus you can see <F5> or To start a measurement
the menu path here. The right side of the header <Start>
displays current time and date.
<F5> or To abort a measurement.
The output fields display the measuring values and <Cancel>
the actual temperature of the sample. Input fields

H79IB001EN-D 13
6 Putting Abbemat into Operation

Quick access area Input control elements

.

Table 6.2: Button functions in the quick access area

Button Function
Open the message list
The general instrument status as well
as all measuring errors that have oc-
curred during the measurement are
described in this list. The button Check box
changes its appearance depending Input box
on the current status.
Drop-down box
Green OK sign
The general instrument status is free
of errors.
Yellow warning sign
The instrument has a minor problem,
for instance if there is a printer prob-
Fig. 6-1: Examples for check box, input box and drop-
lem. down box
Red error sign
The instrument has a major problem To use drop-down menus
that needs to be fixed before you con-
Tap on the drop-down box. Then select an item from
tinue with measurements.
the list.
Adjustment
See section 9.3. To use check boxes
Water Adjustment Tap on a check box to activate or deactivate the re-
See section 9.3. quired function.To enter characters into an input box
Activate the monitor mode Tap on an input box to open the on-screen key-
If you have started a measurement by board. Enter characters, numbers or special charac-
tapping <Start>, the instrument dis- ters by tapping the buttons on the screen and then
plays continuous measuring values tap <OK>.
until the measurement is finished. The
final values are frozen until the next Table 6-1: Functions of the special buttons in
measurement is started. Use this but- the on-screen keyboard
ton to switch back to monitor mode.
Monitor mode is active Deletes the character on the left
Until a measurement is started (or af- side of the cursor position.
ter a measurement is terminated by Moves the cursor position to the
tapping <Cancel>), the instrument is left or right.
in the monitor mode and shows the
current measuring values. Changes to upper case or to
special characters.
To exit dialog windows with or without saving Changes to the numeric
character mode.
You have three options to exit a dialog window:
Changes to alphabetic character
• To get directly to the main screen, tap <Home>
mode.
and decide whether you want to save or discard
changes in the following pop-up window. Changes to additional special
characters.
• To get one menu level higher and save
changes, tap <OK>. To display exponential numbers,
e.g. to enter 0.025 as
• To get one menu level higher without saving
changes, tap <Cancel> 2.5 ^-2 (only in number mode).

14 H79IB001EN-D
 7 Installing Optional Input/Output Devices

7 Installing Optional Input/Output Devices

In this chapter, you find information about the instal- To connect an RS-232 printer
lation of optional input/output devices. For detailed
information on the devices, see the respective man- 1. Connect the printer (e.g. Anton Paar Mat. No.
ual. 44737 or 93362) to the RS-232 interface (COM)
of the instrument using the cable that is supplied
NOTICE together with the printer.
If you use accessories that are not supplied by
2. If you use an RS-232 printer which is not sup-
Anton Paar, we do not guarantee the functional-
plied by Anton Paar, configure the communica-
ity and safety.
tion settings on the printer accordingly.

To connect an office printer via USB interface

7.1 External Keyboard, Bar Code
Reader, Mouse Connect the printer (not supplied by Anton Paar) to
a USB port on the rear side of the instrument.
Connect the external keyboard, bar code reader or
mouse (not supplied by Anton Paar) to one of the To connect an office printer via local network
two USB ports that are located on the rear of the in-
strument. You can connect your instrument and a PCL
TIP: A bar code reader can be used to fill in input compatible printer (or an Epson Stylus D120) via
boxes, for instance a sample name or a user-de- Ethernet to your local network.
fined data field.
1. Connect the instrument via Ethernet interface to
your local network.
7.2 USB Memory Device
2. Configure the network settings according to
section 8.3.
Insert a USB memory device into one of the two
USB ports that are located on the rear side of the in- 3. Connect the printer to your local network. For
strument. details, see the printer instruction manual.
TIP: TFormat the memory devices using FAT,
FAT32 or exFAT. 7.3.2 Installing, Editing, Deleting a Printer
To install a new printer/edit an existing printer
7.3 Printer
1. Tap "Menu > Export Settings > Export Man-
To print the measuring results you can use RS-232 agement" to open the printer list.
printers via RS-232 interface or office printers (PCL 2. Tap <More> and <New> to install a new printer
compatible) via USB or local network. Besides these or tap on an existing printer to edit.
hardware printers you can configure a virtual printer
named "PDF File Printer". This printer is used to ex- 3. For RS-232 printers, enter the following set-
port PDF files to USB memory devices. tings:
- Select the printer "Type" RS-232 Printer.
7.3.1 Connecting a Printer - Activate/deactivate the check box "Use as
default printer".
This section describes how to connect the different - Enter a "Name" for the printer.
printer types to your instrument. After connecting a
printer to the instrument, you have to configure this
printer (see section 7.3.2).

H79IB001EN-D 15
7 Installing Optional Input/Output Devices

4. For USB and network printers, enter the follow- 7.3.4 Defining the Name and Address of the
ing settings:
Report
- Select the required printer "Type".
- Activate/deactivate the check box "Use as 1. Tap "Menu > Export Settings > Name and
default printer". Address".
- Enter a "Name" for the printer.
- Select the paper "Format". 2. Enter the name and address for the report
- Select the "Port" USB or network. 3. Tap <OK> to save the changes.
- Activate/deactivate the check box "Color
Print".
- For network printers only: Enter the IP 7.3.5 Defining the Logos of the Report
address and IP port.
You can import logos via USB memory device and
5. For a virtual PDF file printer, enter the following use them as header or background logo in the print-
settings: er report. To be recognized by the instrument and fit
- Select the printer "Type" PDF file printer. into the document layout, the logo graphic files have
- Activate/deactivate the check box "Use as to:
default printer". - have the file format .jpg or .bmp,
- Enter a "Name" for the printer. - be located in the main directory of the USB
- Select the paper "Format". memory device,
6. Tap <OK> to save the changes. - have a maximum size of 1536 x 1024 pixel.

To print reports on RS-232 printers in english To import logos into the internal memory
language 1. Tap "Menu > Export Settings > Import
Tap "Menu > Control Panel > RS-232 Settings". Logos".
Activate the check box "Always print report in en- 2. Select the "storage location" (USB memory
glish". device).

To delete a printer 3. Select the <File Name> of the image file.

1. Tap <Menu> and select "Export Settings > 4. Tap <Place> to define the position of the image
Export Management" to open the printer list. in the internal memory.

2. Tap <More> and <Delete>. 5. Tap <OK> to load the file into the instrument.

3. Select the printer to delete. To select logos for the report

4. Tap <Delete> and <Yes>. You can select a logo for the report header and a
second logo for the report background.
7.3.3 Setting Automatic Export 1. Tap "Menu > Export Settings > Select
Logos".
The instrument can send the measurement data to
2. Use the two drop-down boxes to select one logo
the printer after each measurement:
for the header and a second logo for the back-
1. Tap "Menu > Export Settings > Automatic ground.
Export".
2. Tap <Output target> and select one of the fol-
lowing:
- <PDF Export> to file
- A printer that has been configured according
to section 7.3.
- <None> to deactivate the automatic export
3. Tap <OK> to save the changes.

16 H79IB001EN-D
 8 General Settings

8 General Settings
8.1 Date and Time 8.5 LIMS Settings
1. Tap "Menu > Control Panel > Date and Time". Abbemat 3200 can be connected to a Laboratory In-
2. Enter the current date and time. The date and formation Management System (LIMS) using the
time format depends on the regional settings. Anton Paar software LIMS Bridge. The LIMS Bridge
can be used to send remote measurement com-
mands from the LIMS to the instrument and to for-
8.2 Regional Settings ward result files from the instrument to the LIMS.

1. Tap "Menu > Control Panel > Regional Set- Abbemat 3200 does not offer any system security
tings". functions according to 21 CFR Part 11, therefore the
2. Select "Language", "Data Format" (numbers, export of checks, adjustments and audit trail via
date and time format,), and "Keyboard Layout". LIMS Bridge is not available.
3. Tap <OK>. 1. Tap "Menu > Control Panel > LIMS Settings".
4. The instrument displays "Are you sure you want 2. Activate/deactivate the check box "Run LIMS
to change the regional settings? The instrument Server on startup".
will reboot to complete this action". 3. Select the "LIMS Measurement Report Format"
5. Tap <Yes> to implement the new settings. The in compact or detail.
instrument reboots. 4. Tap <OK> twice. The instrument will reboot.
To set the instrument to english language you can TIP: To establish a connection with LIMS Bridge,
also connect a USB Keyboard and press <Ctrl>, enter the user name "lims" and the password "lims"
<Alt> and <E> simultaneously. Switch the instru- (without quotes).
ment off and on again.
For more details, see the LIMS Bridge instruction
manual.
8.3 Network
If you connect the instrument to a local network, you 8.6 Unit Settings
can use a network printer for printouts or connect to
a LIMS (see section 8.5). Tap "Menu > Control Panel > Unit Settings" and
1. Tap "Menu > Control Panel > Network". select the units for refractive index and temperature.
2. If your network server has DHCP functionality,
activate "Obtain an IP address automatically 8.7 Display Brightness
(DHCP)".
3. If your network server has no DHCP functional- Tap "Menu > Control Panel > Display Bright-
ity: ness". and select the preferred setting (from 30% to
- Deactivate "Obtain an IP address automati- 100%).
cally (DHCP)".
- Enter "IP Address", "Subnet Mask", "Default
Gateway", "Primary DNS" and "Secondary
8.8 Screen Saver
DNS". The screen saver is active by default and switches
off the display back-light after 30 minutes of
8.4 Instrument Name and Location inactivity. It does not start during measurements,
checks or adjustments. Mouse, keyboard, or touch
If you have more than one instrument and want to activities end the screen saver.
differentiate between them, you can define instru-
ment names and locations: To set the screen saver tap "Menu > Control Panel
1. Tap "Menu > Control Panel > Instrument > Screen Saver".
Name and Location".
2. Enter the "Instrument Name" and "Instrument
Location".

H79IB001EN-D 17
9 Checking, Adjusting and Calibrating

9 Checking, Adjusting and Calibrating

9.1 Definitions TIPS:
• Different batches of the same reference sub-
Checking stance can vary in their refractive index.
Although this difference usually is small, checks
Checking the correct state of operation of an instru- and adjustments have to be performed with the
ment by measuring a sample of exactly known mea- exact value of the standard. Especially when
surement properties and comparing the result with receiving new substances, pay attention to the
the expected values. refractive index.
Adjusting • Always check the expiration date of the refer-
ence liquids.
Enabling correct measurements in the future by
measuring a sample of exactly known measurement • Use a new and clean pipette for every measure-
properties (standard) and adjusting the instrument’s ment.
constants in a way that the known correct results are • Use the reference liquids immediately after
found by the instrument. opening the vial. Close the vial between mea-
surements.
Calibrating
• Used liquid must not be returned into the vial,
Calibrations are checking procedures which are car- except for disposal of the whole vial.
ried out using certified standards. By comparing the
measured result with the standard reference value, • When calibrating manually, perform a series of
you can validate the quality of your measurements. at least three measurements and take the arith-
metic average of the results.

WARNING 9.2 Refractive Index Checks

Before applying any sample or cleaning liquid to With refractive index checks, carried out in regular
your instrument make sure that: intervals, you can ensure a high and stable accura-
cy of your refractive index and concentration mea-
• All safety instructions concerning the use of
surements.
chemicals and the use of inflammable chemi-
cals are met (see section 1). Perform the check at the defined reference tem-
perature of the reference liquid. Compare the mea-
• No source of ignition exists close to the
sured refractive index with the reference value.
instrument.
A water check should be performed every day be-
• Sufficient ventilation is guaranteed.
fore starting measurements. Perform additional wa-
• All wetted parts of the instrument are resis- ter checks on demand depending on your judgment,
tant (see appendix A.2). e.g. when you get a series of unexpected results.
If the check failed, take corrective actions until the
check is valid again:
CAUTION
• Check the reference liquid quality
Hot surface
The measuring prism and its surrounding steel • Clean the measuring prism thoroughly. Alcohol,
surface can get very hot. Do not touch these sur- acetone or a solvent suitable for the samples
faces without adequate protective measures. measured before could be useful.
• If above actions do not help to perform a valid
check, perform an adjustment.

18 H79IB001EN-D
 9 Checking, Adjusting and Calibrating

9.3 Adjustments 4. Enter the reference temperature of the refer-

ence liquid.
5. Enter the name of the reference substance.
9.3.1 Setting the Water Reference
6. Enter reference value of the reference liquid.
The setting of the water reference has to be per- 7. Activate/deactivate a reminder. Define a time
formed, if the water check failed and using fresh interval in days for periodical adjustments.
pure water and cleaning of the measuring prism did
not help. 8. Press <Start>. The adjustment procedure can
be aborted any time by pressing <Cancel>.
To set the water reference preferably use ultra pure
water (alternatively deionized or double distilled wa- 9. Clean and dry the measuring prism (see
ter). section 13.2).

TIPS: 10.Apply the reference liquid to the measuring

prism.
• The adjustment has to be done at measuring
temperature, e.g. 20.00 °C. 11. Attach the sample cover to avoid evaporation
and to prevent ambient light from entering the
• Perform the setting of the water reference when optical system.
changing the measuring temperature.
12.Tap < >. The old and new values will be dis-
• If the temperature changes by more than 3 °C played once the adjustment measurement is fin-
the water reference has to be set. ished.

To set the water reference 13.Choose to <Reject> or to <Accept> this adjust-

ment.
1. Clean and dry the measuring prism (see
section 13.2)
2. Tap < >.
9.3.3 Calibrating the Sample Illumination
3. Apply pure water, attach the sample cover and If the water adjustment is not satisfactory and read-
press <OK>. ings are not stable, a calibration of the sample illu-
mination has to be performed. For this calibration
4. Wait until the button shows < > again. The
the measuring prism has to be absolutely clean and
adjustment procedure can be aborted any time
dry.
by pressing <Cancel>.
TIP: If the ambient temperature is close to the dew
point (high relative humidity), the calibration of the
9.3.2 One Point Adjustments sample illumination should be performed at a higher
set temperature, e.g. 30 °C in order to avoid dew on
If all results across the entire measuring range are the measuring prism.
systematically too high or too low, this deviation can
be corrected by performing a one point adjustment 1. Tap <Menu> and select "Service > Calibrate
(Abbemat 3100/3200 only) Sample Illumination".

TIPS: 2. Clean the prism thoroughly according to

section 13.2.
• A one point adjustment modifies the axis inter-
cept of the internal calibration curve. 3. Confirm with <Yes> and follow the instructions
on the screen.
• The adjustment has to be done at the certified
reference temperature of the standard, e.g.
20.00 °C. 9.3.4 Reset to Factory Adjustment
To perform a one point adjustment You can reset the adjustment of the instrument to
the factory adjustment as follows:
1. Press < >. A window displays information
about the last adjustment. 1. Tap <Menu> and select "Service > Reset to
Factory Adjustment".
2. Press < > to set the adjustment parameters.
2. Confirm with <Yes>.
3. Select "One Point Adjustment".

H79IB001EN-D 19
10 Methods

10 Methods
The Abbemat refractometers are used in all indus- 4. Enter a <Hold Time> (see section 11.6).
tries, from pharmaceuticals, chemicals, flavors and
5. Add a user-defined data field to every measure-
fragrances to beverages and food. Your instrument
ment by activating the check box. You can also
is delivered with a set of predefined methods cover-
enter a name (e.g. "Batch Number") for this
ing the most common applications.
field, which is displayed on the main screen.
In close cooperation with our customers Anton Paar
6. Define mandatory data input for the sample
collects and develops continuously new methods
name or the user defined field:
and applications. Benefit from the constant growing
method know-how and contact Anton Paar for an - Choose between <Not Mandatory>, <Man-
updated list of available methods. datory - Keep Value> and <Mandatory -
Clear Value>.
- With the option <Clear value> the input field
10.1 Changing Method Parameters is cleared every time when opened. The
option <Keep Value> preserves the last input
Each method contains the following parameters: value.
- Mandatory fields are marked with a red line
• Method name
on the main screen and have to be com-
• Hold time, see section 11.6 pleted before a measurement can be per-
formed.
• User defined field
7. Tap <OK>.
• Mandatory data input
With the user defined field you can assign additional
information to your samples. Typical examples are 10.2 Installing a Method Package
a filling line number, a tank number or a batch iden-
tification. In close cooperation with our customers Anton Paar
collects and develops continuously new methods
Furthermore, the clearing of input fields after each
and applications. Benefit from the constant growing
data recording can be enabled, and data input can
method know-how and contact Anton Paar for an
be defined as mandatory. If activated, a measure-
updated list of available methods.
ment is only possible after data is entered in the cor-
responding input fields, ensuring that no “blank data 1. Load the method pack onto your USB memory
sets” are recorded. device.
2. Insert the USB memory device into a USB port
To configure a method
of your instrument.
1. Tap <Method>.
3. Tap "Menu > Service > Update > Install
2. Tap the settings icon < > located next to the Method Package".
method or empty slot you would like to change.
4. Select the "USB memory device".
3. To change the method in this slot use the drop-
5. Select a method package file.
down box <Method>.
6. Confirm with <OK>.

20 H79IB001EN-D
 11 Measuring

11 Measuring
In this chapter, the filling of samples and the mea-
suring procedure are described.
11.2 Selecting a Method
Tap <Method> on the main screen to open the
methods list and select a method (see section 10).
WARNING
Before applying any sample or cleaning liquid to
your instrument make sure that:
11.3 Applying a Sample
• All safety instructions concerning the use of
chemicals and the use of inflammable chemi-
cals are met (see section 1).
• No source of ignition exists close to the
instrument.
• Sufficient ventilation is guaranteed.
• All wetted parts of the instrument are resis-
tant (see appendix A.2).

CAUTION
Hot surface
The measuring prism and its surrounding steel
surface can get very hot. Do not touch these sur-
faces without adequate protective measures.
The Abbemat prism has a circular, polished measur-
ing surface (prism) on which the sample has to be
11.1 Automatic Temperature Control placed. Liquids as well as pasty samples can be
measured. Pasty samples should be applied with a
Abbemat 3100 and Abbemat 3200 are equipped spatula. Samples of low surface tension such as al-
with an automatic peltier temperature control provid- coholic solutions cover the prism and the surround-
ing a fast and precise automatic temperature control ing sample well. Therefore a higher volume is
of the sample. necessary to reach the required filling height of at
least 1 mm. For convenient dosage of liquid sam-
Tip: It is recommended to perform measurements ples, polyethylene pipettes are recommended.
with automatic temperature control, because the
sample temperature is one of the major external in-
fluencing parameters in refractive index measure- 11.4 Magnetic Sample Cover
ments.
The temperature control mode of the Abbemat 3100 The magnetic sample cover should always be used.
is selected with the radio buttons <20 °C/68 °F> or The O-ring seals the measuring area so that the
<25 °C/77 °F>. Disable the temperature control with evaporation of volatile substances is reduced.
the button <-- °C/°F>:

101659
The temperature control mode of the Abbemat 3200
can be activated by using the check box. Tap <Set
Temperature> to enter the required set tempera-
ture: 92654

H79IB001EN-D 21
11 Measuring

11.5 Performing Measurements 11.6 Measurement Accuracy

1. Before starting a measurement, check that: The Abbemat 3000/3100/3200 determines the va-
lidity of a measurement based on different criteria,
- the measuring prism is clean (section 13.2)
which are checked during the measurement pro-
and dry
cess.
- the correct sample name is entered
- the method settings are as required If temperature control is enabled (Abbemat 3100/
(section 10.1) 3200 only), the instrument adjusts the temperature
- suitable cleaning liquids are available of the sample to the set temperature. After tempera-
(section 13.2) ture equilibration a Hold Time period starts (default
5 s). This additional time for the adaption of the
2. Apply the sample so that the whole prism is cov-
sample temperature improves the stability of the re-
ered. The required sample volume is approxi-
fractive index measurement.
mately 1 mL. The filling height should be at least
1 mm above the prism surface. Make sure that Highly viscous samples for instance might require a
no air bubbles are formed on the prism surface. longer time for temperature equilibration inside the
liquid. Extending the hold time can help compensat-
3. Attach the sample cover to avoid evaporation
ing this lag.
and to prevent ambient light from entering the
optical system. When the hold time has passed and the sample
temperature remains stable, three different parame-
4. After the sample has been applied on the prism,
ters determine the outcome of a measurement (see
some time is required until the sample has
table 11-1).
attained the prism temperature and a constant
result is displayed. • Max. temperature deviation: The maximum
allowable deviation of the sample temperature
Tip: The smaller the sample volume, the shorter
to give a valid measurement. Active during the
the stabilization time. Samples of good thermal con-
whole measurement process if temperature
ductivity yield stable readings in about 15 to 20 sec-
control is active.
onds, while samples of poor thermal conductivity
such as syrups and other solutions of high viscosity • Stable time: During this period the refractive
may require one minute or more. index must be stable to give a valid measure-
ment.
5. Press <Start> and wait until the measurement is
finished. The progress bar shows the progress • Max. refractive index deviation: The maxi-
of the measurement. When the measurement is mum allowable deviation of the refractive index
finished, the progress bar turns to green. The within the stable time interval to give a valid
condition output field shows the message measurement.
"valid".
If the refractive index is not stable within the Time-
6. The result values are saved in the data memory out interval of 120 s, the measurement will be abort-
and can be viewed, printed, exported or deleted. ed.
7. Clean and dry the measuring prism (see
section 13.2) and measure the next sample.

Table 11-1: Accuracy for the validation of measurements and adjustments

Measurement Adjustment

Max. temperature deviation 0.005 °C

(Abbemat 3100/3200 only)

Stable time 5s 15 s

Max. refractive index deviation

with temperature control 0.00002 nD 0.00001 nD
without temperature control 0.00003 nD 0.000025 nD

22 H79IB001EN-D
 12 Handling the Measurement Data

12 Handling the Measurement Data

12.1 Data Memory Data can be exported in three different file formats:
• MS Excel spreadsheet (.xls)
Your instrument can store 2500 result records. After
• Text (tabulator separated values)
1000 measurements, the instrument issues a re-
minder to clear the memory. If the memory is full, no • PDF (portable document format)
further measurements are possible. Make sure to
Three different report types are available for printout
free memory space in time.
and export:
• Compact: The most important data of a single
12.2 Viewing Results measurement.
• Detail: All available data of a single result on a
Your instrument supports two forms of result visual-
single page.
ization:
• Table: Multiple measurements in table form.
• In the multiple sample view you can see a num-
ber of results listed in a table.
• In the single sample view you can see a more 12.4 Printing or Exporting Data
detailed view of one result at a time.
To print the measuring results you can use RS-232
Activating the multiple sample view printers via RS-232 interface or office printers (PCL
1. Tap <Meas. Data> to open the multiple sample compatible) via USB or local network. Besides these
view. hardware printers you can configure a virtual printer
named "PDF File Printer". This printer is used to ex-
2. Use the scrollbar to scroll through the list. port PDF files to USB memory devices. For the con-
figuration of printers see section 7.3.
Activating the single sample view
1. Tap <Meas. Data> to open the multiple sample
1. Tap <Meas. Data> to open the multiple sample view.
view.
2. Tap <More> and then tap <Export>.
2. Tap on a sample result to open the single sam-
ple view. 3. Select the items you wish to print or export and
then tap <Export>.
3. Use the <up> or <down> button to see the previ-
ous or next result. 4. Use the drop-down boxes “Output Target”,
“Reports”, “Storage Location” and “File Name”
to define the export settings.
12.3 Print and Export Options 5. Tap <OK>.

The instrument supports three kinds of printouts:

• Continuous printouts using an RS-232 printer
12.5 Deleting Data
with paper roll.
We recommend deleting your result data regularly
• Report printouts using an office printer (USB or after a successful data export or printout.
local network).
1. Tap <Meas. Data> to open the multiple sample
• Report printouts to PDF files (USB flash drive). view.
2. Tap <More> and <Delete>.
3. Select the items to delete or tap <Select all>.
4. Tap <Delete>.

H79IB001EN-D 23
13 Upkeep and Cleaning

13 Upkeep and Cleaning

To ensure the high accuracy of your measurements,
employ a regular cleaning routine and store the in-
13.2 Cleaning the Measuring Prism
strument under the recommended conditions.
1. Pure tissue paper is best suited for wiping off
the samples. Do not use wet wipes or coated tis-
sues. Use a new tissue for each cleaning step.
WARNING Avoid touching the prism with your fingers or
cleaning the prism with a part of the tissue that
Before applying any sample or cleaning liquid to
was touched before.
your instrument make sure that:
2. The prism surface must be clean and dry before
• All safety instructions concerning the use of the sample is applied.
chemicals and the use of inflammable chemi-
3. Occasionally cleaning of the prism with water or
cals are met (see section 1).
another suitable solvent such as ethanol or ace-
• No source of ignition exists close to the tone may be necessary.
instrument.
• Sufficient ventilation is guaranteed.
• All wetted parts of the instrument are resis- 13.3 Drying Cartridge
tant (see appendix A.2).
A drying cartridge at the rear of the measuring unit
(Abbemat 3100/3200 only) protects the optical sys-
tem from moisture. The cartridge is equipped with a
CAUTION
humidity indicator behind the window in the center.
Hot surface This allows you to check the status of the cartridge
The measuring prism and its surrounding steel without the need to remove it.
surface can get very hot. Do not touch these sur-
faces without adequate protective measures. NOTICE
• Check the status of the drying cartridge at
least once a year.
NOTICE
To protect the surface of your instrument never • Don’t exchange the cartridge unless it needs
use: exchanging.
• Highly nonpolar solvents (e.g. toluene, • Always install a drying cartridge. An unsealed
hexane, solvent naphtha) optical system might result in damage to the
• Strong acids or bases (e.g. nitric acid, instrument.
sulfuric acid, hydrochloric acid, caustic soda)
• Strong mechanical action (e.g. steel brush). When the desiccant in the cartridge saturates with
moisture, the indicator changes its color from blue to
pale pink. If the blue color disappears and the indi-
13.1 Cleaning Instrument Housing and cator begins to show a tinge of pink, the cartridge
must be replaced:
Touchscreen
To clean the instrument housing or the touch screen,
use a soft tissue that can be wetted with an ethanol
water mix or warm water, if necessary with some
mild cleaning agent added (pH < 10).

Cleaning frequency for ventilation openings

1 2 3
All ventilation openings must be cleaned at least
Fig. 13-1: Humidity indicator of drying cartridge
once a year with a vacuum cleaner. Reduce the vac-
uum power to avoid high fan speeds. Avoid touching 1 Dry, new cartridge
the fan blades in order to protect the fan bearing and 2 Replace
3 Saturated, replace immediately
blades.

24 H79IB001EN-D
 13 Upkeep and Cleaning

To replace a drying cartridge

1. Unscrew the old cartridge counterclockwise
using the transparent, protective cap of the new
cartridge as a screwdriver.
2. Remove any residue around the threaded hole
of the housing.
3. Check the correct position of the gasket of the
fresh cartridge.
4. Screw the cartridge into the hole.

13.4 Storing the Instrument

Always clean and dry the prism before storing the in-
strument for more than one day. For a storage that
lasts less than one day, you can leave a few drops
of distilled or demineralized water on the prism and
attach the sample cover.

H79IB001EN-D 25
14 Maintenance and Repair

14 Maintenance and Repair

14.1 Warranty 14.2 Repair Performed by an Autho-
To keep the warranty1 on the instrument valid, ob-
rized Anton Paar Representative
serve the instructions given in section 13, "Upkeep
and Cleaning". In case your instrument needs repair, contact your
local Anton Paar representative, who will take care
The instrument does not require any periodic main- of the necessary steps. If your instrument needs to
tenance by Anton Paar service engineers to retain be returned, request an RMA (Return Material Au-
the warranty. thorization Number). It must not be sent without the
RMA and the filled “Safety Declaration for Instru-
The following wear and tear parts are generally ment Repairs”. Please make sure it is cleaned be-
excluded from the warranty: fore return.
• Drying cartridge TIP: Find the contact data of your local Anton Paar
• Fuses representative on the Anton Paar website (http://
www.anton-paar.com) under “Contact”.
• Hoses in direct contact with sample (if applica-
ble, i.e. for flow cells or peristaltic pumps)
• O-rings in direct contact with sample (if applica-
ble, i.e. for flow cells)
• Fluid connectors in direct contact with sample (if
applicable, i.e. UNC connectors for flow cells)
• Button cell (real-time clock)
The real-time clock of the device is powered by an
internal lithium button cell while the device is
switched off. When the button cell is depleted, the
real-time clock sets the date of the instrument to
January 1, 2000 on start-up. If date and time are not
set manually, incorrect time stamps will be written to
reports.
To ensure continuous operation, it is recommended
to replace the button cell every five years as a pre-
ventive measure. Contact your local Anton Paar
representative for a replacement of the button cell.

1 For detailed information please see the general terms of delivery (GTD) on the Anton Paar website
(http://www.anton-paar.com).

26 H79IB001EN-D
 15 Updates and System Information

15 Updates and System Information

15.1 Updating the Instrument Software 15.3 Saving an Instrument Log File
1. Load the latest instrument software onto your You can save an instrument log file to a USB mem-
USB memory device. ory device. This file contains the last operating steps
and may help during trouble shooting.
2. Insert the USB memory device into a USB port
of your instrument. To save the instrument log file
3. Tap "Menu > Service > Update > System
1. Tap "Menu > Service > System Information"
Update".
to open the configuration overview table.
4. Select the <USB memory device> and the <File
2. Insert a USB memory device into a USB port
Name> of the new firmware.
and press <Save Logfile>.
5. Press <OK>. and follow the instructions on the
3. Accept the automatically generated file name or
screen.
enter a new one and press <OK>. The instru-
ment log file is now saved to the main directory
15.2 System Information of the USB memory device.

In this menu, you find information about the config-

uration of the hardware and firmware. The instru-
15.4 Live Raw Data
ment type, serial number and firmware versions are
Tap "Menu > Service > Live Raw Data" to get a
shown.
live view of sensor signals including raw data.
To view hardware and firmware configuration
1. Tap "Menu > Service > System Information"
to open the configuration overview table.
2. Use the <Up> and <Down> keys to scroll
through the table.

H79IB001EN-D 27
Appendix A: Technical Data

Appendix A: Technical Data

Appendix A.1: General Technical Data
Dimensions (W x D x H) 228 mm x 300 mm x 97 mm
Weight 3803 g (Abbemat 3000)
3950 g (Abbemat 3100/3200)
680 g (Power supply with cable)
Mains voltage From 100 VAC to 240 VAC, 50/60 Hz
Power consumption Max. 120 VA
Power inlet according to IEC/EN 60320-1/C14, protection class I
AC adapter output DC 24 V, max. 5 A
Housing material:
Front, top & side cover Styrene/Butadiene
Back, bottom, drip plate, measuring area Stainless steel
Measuring prism Synthetic Sapphire
Environmental conditions (EN 61010) Indoor use only
Ambient temperature +10 °C to +35 °C (+50 °F to +95 °F)
Air humidity 10 % to 90 % relative humidity, non-condensing
Light source LED, average life time of 100 000 h
Wavelength 589.3 nm
Touchscreen display 145 mm (5.7"), 640 x 480 pixel
Memory 2500 measuring values
Interfaces • 2 x USB 2.0
• 1 x USB 2.0 type B (service)
• 1 x Ethernet/LAN
• 1 x RS-232

NOTICE
• Only connect devices to the interfaces that comply with PELV (protective extra-low voltage) accord-
ing to EN 61140 or with SELV (safety extra-low voltage) according to EN 60950.
• Only connect USB devices with a maximum current load of 500 mA to the USB ports.

Appendix A.2: Wetted Parts

The following parts are in contact with samples and cleaning liquids:
Measuring prism Synthetic Sapphire
Sample well (surrounding the prism) Stainless steel
Drip plate (surrounding the sample well) Stainless steel
Seal between prism and sample well FFKM
Seal between sample well and drip plate FFKM (Abbemat 3100/3200)
FKM (Abbemat 3000)
Center inlay of magnetic sample cover PPSU
O-ring of magnetic sample cover FKM

28 H79IB001EN-D
 Appendix A: Technical Data

Appendix A.3: Specifications

Abbemat 3200 Abbemat 3100 Abbemat 3000
Measuring range [nD] 1.30 to 1.72 1.30 to 1.66
Resolution [nD] 0.0001
Uncertaintya) [nD] +/-0.0001
Temperature control range [°C] 15b) to 60 20b) or 25 -
Resolution [°C] 0.01 °C 0.01 °C 0.01 °C
Uncertainty of temperature +/-0.05
a)
measurement [°C]
Stability 0.0025 °C -

a) at refractometric standard conditions (T=20 °C, λ=589 nm, ambient temperature=23 °C).
b) at an ambient temperature of max. 35 °C

Appendix A.4: Menu Tree

Level 1 Level 2 Level 3
Control Panel Date and Time
Regional Settings
Network
Instrument Name and Location
LIMS Settingsa)
Unit Settings
Display Brightness
Screen Saver
RS-232 Settings
Export Settings Export Management
Automatic Export
Name and Address
Select Logos
Import Logos
Service Update System Update
Install Method Package
System Information
Reset to Factory Adjustment
Calibrate Sample Illumination
Reset Unique Sample ID
Live Raw Data
Maintenance Login (password)
Unlock Service

a) Abbemat 3200 only

H79IB001EN-D 29
Appendix B: Troubleshooting

Appendix B: Troubleshooting
Table 15-1: Display of unstable results
Problem Cause Correction

Sedimentation on the prism Sample contains particles, e.g. Filtrate the sample
fibers

Sample is hygroscopic or The sample is absorbing Use a sample cover with O-ring
contains a solvent of low vapor humidity or gas from the air or seal
pressure solvent is evaporating from the
sample

Residue of previously measured The samples will mix slowly Clean the prism thoroughly
sample has remained on prism before measuring

Table 15-2: Measuring problems

Problem Cause Correction

The instrument cannot get a Refractive index of the sample Measure sample with its
measuring result is out of range refractive index in range of
instrument

Table 15-3: Temperature Problems

Problem Cause Correction

The instrument is overheated Insufficient air circulation Ensure unhindered air

circulation at the ventilation
openings

Ambient temperature too high Lower the room temperature

Frequent and fast changes Extend the times between

between high and low prism different prism temperatures
temperatures

Defective cooling fan Contact your local Anton Paar

representative

Malfunction of internal Contact your local Anton Paar

temperature control representative

Prism temperature is not stable Temperature control is Switch temperature control on

switched off

Internal Peltier temperature Ensure unhindered air

control is not reaching the set circulation at the ventilation
temperature openings; if required, lower
room temperature

Malfunction of internal Contact your local Anton Paar

temperature control representative

30 H79IB001EN-D
 Appendix C: CE Certificate

Appendix C: CE Certificate

H79IB001EN-D 31