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Sterilization: Vertical Autoclaves Without Drying

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0% found this document useful (0 votes)
137 views12 pages

Sterilization: Vertical Autoclaves Without Drying

Uploaded by

samson
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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VERTICAL

AUTOCLAVES
Sterilization WITHOUT DRYING
AES SERIES CLASSIC LINE

ECONOMIC, DURABLE,
ROBUST PERFORMANCE
AND LIMITED LABORATORY
RESOURCES CONSUMPTION
Vertical autoclaves without drying
The AES Series vertical floor-standing autoclaves with
top-loading access cover the fundamental needs for general INTENDED USE
labware sterilization in many industries, educational institutions
and research facilities with the aim of increasing the productivity + STERILIZATION OF
LABORATORY WASTE
of the laboratory. A great chamber capacity together with the BAGS, PLASTICS,
CULTURE MEDIA,
optimization of resources such as water, power and operating GLASSWARE, LIQUIDS
time results in an affordable and efficient solution to manage AND METAL UTENSILS

laboratory workload.

MAIN FEATURES

ECONOMIC AND DURABLE MULTIPLE TYPES OF EASY INSTALLATION AND SAFETY FIRST
STERILIZATION CYCLES MAINTENANCE

AES Series autoclaves Several options available Every AES Series autoclave AES Series autoclaves are
are economic and robust to perform solids or liquids is a plug and play equipment equipped with several features
autoclaves with excellent sterilization. Optional flexible that do not need dedicated to ensure the safety of the
performance for general temperature probe for load installation connections. They operators. These include an
laboratory sterilization sensed sterilization of liquids, simply need a power source overpressure safety valve,
procedures. They can be used temperature holding at the and can work even without a a thermally insulated door,
either for solids and liquids end of the sterilization cycle connection to the drainage. an overtemperature safety
sterilization procedures and for culture media and manual All models include casters so thermostat, an open door
they consume limited valuable unsteaming push-button for a they can be used in different detection system and an
laboratory resources such as faster cooling phase of solids. areas of the same facility. independent safety pneumatic
water, power or operator time. system that locks the main
door while positive pressure
exists inside the sterilization
chamber.
2 VERTICAL AUTOCLAVES WITHOUT DRYING - AES SERIES
ADVANTAGES

Sterilization chamber and door made of Available special models with augmented
high quality stainless steel grade AISI-316L power to achieve faster heating and
extremely resistant to corrosion. sterilization phases.

Equipment built following all applicable Adjustable temperature holding at the end of
European Union quality, regulatory and safety the sterilization cycle between 40-80ºC (Agar
standards. mode).

Heating by powerful electric elements made of Programmable auto-start for up to 24h.


Incoloy® 825 assembled inside the sterilization
chamber and shielded by a protective grid. Optional software for sterilization data
management.
Control by a PID microprocessor with 4
predefined and 6 editable programs, adjustable Optional integrated or external printer.
by time, temperature and type of sterilization
cycle (Agar mode and/or heart temperature Plug and play equipment, no plumbing
probe control). required.

Manual steam release push-button for a faster Easy mobility, all models include casters.
cooling phase in solids sterilization cycles.

STERILIZATION
APPLICATIONS

AES Series autoclaves are intended


for the sterilization of a wide range of
liquids and solids such as culture media,
glassware, plastics, metal utensils, waste
bags and other laboratory items.
These reliable workhorses for the
general laboratory are designed for an
easy operation and include many safety
features to protect users in their daily
routine.

VERTICAL AUTOCLAVES WITHOUT DRYING - AES SERIES 3


Vertical autoclaves without drying

WORKING PRINCIPLE AES Series autoclaves provide a solution for the multiple sterilization needs of general laboratories
including liquids, culture media, biological waste, contaminated media, instruments, glassware and
other laboratory items.

The load has to be placed into the vessel’s baskets and, after manually filling the tank with purified
water, the equipment starts to heat up and purge until the set combination of sterilization time and
sterilization temperature is reached.

DIGITAL MICROPROCESSOR AES SERIES PROGRAMS


Digital microprocessor with 6 push-buttons AES Series autoclaves have 10 programs, from P0 to P9, and the first four are predefined
for an easy programming and parameters and protected.
selection.

PREDEFINED PROGRAMS
Program Sterilization temperature Sterilization time Program mode
Nº ºC min Agar mode or Heart probe
P0 115 60 SOL/LIQ-1
P1 121 30 SOL/LIQ-1
P2 133 20 SOL/LIQ-1
P3 121 20 SOL/LIQ-1

The rest of the programs are editable with the following parameters settings:
• Sterilization temperature.
• Sterilization time.
• Sterilization controlled by main chamber temperature probe or both main chamber temperature
probe plus heart temperature probe.
• Sterilization with temperature holding at the end of the cycle (Agar mode).

STANDARD AES SERIES STERILIZATION CYCLE


HEATING PHASE STERILIZATION PHASE COOLING PHASE
• In this initial step, the powerful heating • Upon reaching the set sterilization • After sterilization phase finishes, natural
elements assembled at the bottom of the temperature inside the chamber the cooling begins and an acoustic beep will
sterilization chamber heat up dramatically, sterilization phase begins, accurately sound when a safety temperature is reached
transferring energy to water to produce sustaining the temperature throughout the and the door can be opened.
saturated steam throughout the chamber. duration of this phase.
• In solid programs, discharge can be manually
• To shorten the duration of this step, RAYPA • This crucial step is controlled by a PT-100 forced through a push-button to reduce the
offers special models with increased Class A temperature probe located within the duration of the cooling phase.
power, a feature of particular interest for chamber. As an option for liquids sterilization • If Agar mode is activated, the equipment
autoclaves operating in laboratories with processes, this phase can be regulated by a will hold the preprogrammed temperature
high workloads. flexible PT-100 Class A temperature probe indefinitely, selectable between 40 and 80ºC.
located inside a sample.

Sterilization cycle graph

A Heating phase
B Sterilization phase
C Cooling phase

Temperature
Pressure

4 VERTICAL AUTOCLAVES WITHOUT DRYING - AES SERIES


FUNCTIONS DISPLAYED
BY THE ALPHANUMERIC
PROG. Nº
LCD SCREEN CURRENT
P0 - P9
The alphanumeric screen apart from STERILIZATION
showing the standard sterilization TIME
PROGRAM
parameters also shows current sterilization
MODE
phase and several visual alerts, including
warning or failure messages. The available SOL/LIQ-1
languages include English, Spanish, French SOL/LIQ-2 CURRENT
and Catalan. For other languages please AGAR-1 STERILIZATION
contact us. TEMPERATURE
AGAR-2

LOADING CAPACITIES

ERLENMEYER FLASKS
Autoclave model AES-28 AES-50 AES-75 AES-110 AES-150
Usable chamber dimensions Ø x H mm 300 x 440 300 x 710 400 x 600 400 x 850 500 x 780
Usable capacity L 31 50 75 110 153
Total baskets 2 3 3 4 4
250 ml
Total units per basket 7 7 12 12 20
(Ø 85 x 143 mm)
Total units 14 21 36 48 80
Total baskets 2 3 2 3 4
500 ml
Total units per basket 4 4 8 8 14
(Ø 105 x 183 mm)
Total units 8 12 16 24 56
Total baskets 1 1 2 3 3
1000 ml
Total units per basket 1 1 4 4 8
(Ø 131 x 230 mm)
Total units 1 1 8 12 24
Total baskets 1 1 1 1 1
2000 ml
Total units per basket 1 1 3 3 5
(Ø 166 x 280 mm)
Total units 1 1 3 3 5

BOTTLES
Autoclave model AES-28 AES-50 AES-75 AES-110 AES-150
Usable chamber dimensions Ø x H mm 300 x 440 300 x 710 400 x 600 400 x 850 500 x 780
Usable capacity L 31 50 75 110 153
Total baskets 2 3 3 4 4
250 ml
Total units per basket 10 10 19 19 30
(Ø 70 x 143 mm)
Total units 20 30 57 76 120
Total baskets 2 3 2 3 4
500 ml
Total units per basket 7 7 12 12 20
(Ø 86 x 182 mm)
Total units 16 24 24 36 80
Total baskets 1 1 2 3 3
1000 ml
Total units per basket 2 2 8 8 14
(Ø 101 x 203 mm)
Total units 2 2 16 24 42
Total baskets 1 1 1 1 1
2000 ml
Total units per basket 1 1 4 4 8
(Ø 136 x 260 mm)
Total units 1 1 4 4 8

*All data on loading capacities of these tables are non-binding guidance to help you choose your autoclave model. The total units per basket and per model have been calculated using standard baskets, for special loads
that require custom baskets please contact us.

VERTICAL AUTOCLAVES WITHOUT DRYING - AES SERIES 5


Vertical autoclaves without drying

Accessories

INTEGRATED BASKETS CRANE


Reference ELEV-CLAV
Dimensions L x D x H mm 800 x 300 x 2100 Intended use
• Powerful lift system with adjustable
Power W 480
arm to assist the movement of heavy
Weight Kg 40 loads into the autoclave.
For autoclaves with the following
79, 115 and 175
chamber volumes L Features
• Ease of use.
Max. load Kg 30
• Compatible with 79L, 115L and 175L
Voltage V 230 vertical autoclaves. Contact us for
Frequency Hz 50/60 other models.
*Must be installed in our facilities. • Up to 200 degrees of rotation.

Safety
• Emergency stop button.
• Motor with auto braking system.

MOBILE BASKETS CRANE


• Electrically operated crane made of stainless steel to assist the loading and unloading of
heavy loads up to 50 Kg.
• Push-button operation control for ease of use.
• With swiveling casters for more maneuverability.
Reference: MOB-LIFT

6 VERTICAL AUTOCLAVES WITHOUT DRYING - AES SERIES


Accessories

STAINLESS STEEL WIRE BASKETS


Reference CV-28 CV-75S CV-75 CV-150S CV-150M
Exterior
270 x 185 370 x 180 370 x 265 470 x 190 470 x 235
Ø x H mm
Dimensions
Interior
260 x 180 360 x 175 360 x 260 460 x 185 460 x 230
Ø x H mm
For 33 L 2 - - - -
autoclaves 55 L 3 - - - -
with the
79 L - 3 2 - -
following
chamber 115 L - 4 3 - -
volumes 175 L - - - 4 3

STAINLESS STEEL LIQUIDS COLLECTOR TRAY FOR WIRE BASKETS


Reference TR-270 TR-370 TR-470
Exterior
240 x 50 320 x 50 420 x 50
Ø x H mm
Dimensions
Interior
238 x 48 318 x 48 418 x 48
Ø x H mm
CV-28  - -
For the following wire
CV-75S & CV-75 -  -
baskets models
CV-150S & CV-150M - - 

UNPERFORATED STAINLESS STEEL BASKETS FOR LABORATORY


WASTE STERILIZATION
Reference CCI-28 CCI-75S CCI-75 CCI-150S CCI-150M
Exterior
270 x 185 370 x 180 370 x 265 470 x 190 470 x 235
Ø x H mm
Dimensions
Interior
260 x 180 360 x 175 360 x 260 460 x 185 460 x 230
Ø x H mm
For 33 L 2 - - - -
autoclaves 55 L 3 - - - -
with the
79 L - 3 2 - -
following
chamber 115 L - 4 3 - -
volumes 175 L - - - 4 3

STAINLESS STEEL “SCHIMMELBUSCH” DRUM FOR MEDICAL


INSTRUMENTS STERILIZATION
Reference TBE-24x16 TBE-34x24 TBE-48x24
Exterior
240 x 165 340 x 240 480 x 240
Ø x H mm
Dimensions
Interior
230 x 155 330 x 230 470 x 230
Ø x H mm
33 L 2 - -
55 L 4 - -
For autoclaves
with the following 79 L - 2 -
chamber volumes
115 L - 3 -
175 L - - 3

VERTICAL AUTOCLAVES WITHOUT DRYING - AES SERIES 7


Vertical autoclaves without drying

Accessories

STAINLESS STEEL CYLINDERS FOR PETRI DISHES STERILIZATION


Reference CEP-1027 CEP-1041 CEP-1427 CEP-1441
Exterior
Dimensions 100 x 270 100 x 410 140 x 270 140 x 410
Ø x H mm
Maximum
number dishes / 10 18 10 18
Petri dishes cylinder
Diameter Ø mm 80 80 120 120
33 L 4 4 2 2
For
autoclaves 55 L 8 4 4 2
with the
79 L 16 8 10 5
following
chamber 115 L 24 16 15 10
volumes
175 L 28 14 16 8

STAINLESS STEEL CYLINDERS FOR PIPETTE STERILIZATION


Reference CEPP-726 CEPP-740 CEPP-1025 CEPP-1435
Exterior
70 x 260 70 x 400 100 x 250 140 x 350
Ø x H mm
Dimensions
Interior
60 x 250 60 x 390 90 x 240 130 x 340
Ø x H mm
33 L 11 11 6 6
For
autoclaves 55 L 22 11 12 12
with the
79 L 42 21 20 10
following
chamber 115 L 63 42 30 20
volumes
175 L 90 30 51 34

STAINLESS STEEL TRAY SUPPORT WITH HEIGHT ADJUSTABLE


TRAYS*
Reference SRA-1 SRA-5 SRA-2 SRA-3 SRA-4 SRA-2-3 SRA-4-3
Dimensions Ext.
270x390 270x680 370x560 370x810 470x740 370x190 470x250
Ø x H mm
Max. number
8 14 11 16 15 4 5
trays / support
Ref. TSRA-1/5 TSRA-1/5 TSRA-2/3 TSRA-2/3 TSRA-4 TSRA-2/3 TSRA-4
Trays Dim.
252x20 252x20 356x20 356x20 454x20 356x20 454x20
Ø x H mm
33 L 1 - - - - - -
For
autoclaves 55 L - 1 - - - - -
with the
79 L - - 1 - - 3 -
following
BSRA
chamber 115 L - - - 1 - 4 - Set of 30 individual
volumes fastening clips.
175 L - - - - 1 - 3

*The purchase of a tray support includes a set of 3 trays and 9 fastening clips. Likewise, the purchase of a tray includes a set of 3 fastening clips.

8 VERTICAL AUTOCLAVES WITHOUT DRYING - AES SERIES


Accessories

FLEXIBLE “HEART” TEMPERATURE PROBE PT-100 CLASS A


• After installing this accessory, the temperature regulation of the sterilization cycle can either
be controlled by the main chamber temperature sensor or both the main chamber temperature
sensor and the temperature sensor of the flexible heart temperature probe.
• The temperature control by the flexible heart temperature probe is especially advantageous for
processes involving the sterilization of large volumes of liquids, where the sterilization process
is regulated by both the temperature achieved in the center of the liquid sample as well as the
temperature achieved in the sterilization chamber. Furthermore, should the autoclave be opened
at chamber temperatures higher than 80ºC there is a risk of liquids boiling over which can be
avoided if the temperature of the sample is controlled throughout the sterilization procedure.
• Must be installed in our facilities.
Reference: PT-2

EXTERNAL TEMPERATURE PROBE ADAPTER INTEGRATED THERMAL PRINTER

• External adapter for continuous • Prints program number, cycle


validation processes that number, temperature, date
allows the access of an and hour of the run and error
external probe (Ø 3-6 mm) to messages.
obtain a temperature reading • Selectable printing cadence
independent from that of the between 10 and 240 seconds.
equipment microprocessor.
• Must be installed in our facilities.
• It is located on the door of the
autoclave. Reference: IT

• Must be installed in our facilities. Consumable:


Paper: PAPER-IT
Reference: EXT-TP

TABLE TOP DOT MATRIX PRINTER INTEGRATED DOT MATRIX PRINTER

• Prints program number, cycle • Prints program number, cycle


number, temperature, date number, temperature, date
and hour of the run and error and hour of the run and error
messages. messages.
• Used with RS-232 connection. • Selectable printing cadence
• Selectable printing cadence between 10 and 240 seconds.
between 10 and 240 seconds. • Must be installed in our facilities.
Reference: ITS References: IT/M
Consumables: Consumables:
Paper: PAPER-ITS, Ribbon: 70945 Paper: PAPER-ITS, Ribbon: 70934

VERTICAL AUTOCLAVES WITHOUT DRYING - AES SERIES 9


Vertical autoclaves without drying

Accessories

PREMIUM CASTERS (2 WITH BRAKES) TRANSPORT TROLLEY

• Although all AES Series • Auxiliary trolley to assist the


autoclaves include casters, this loading and unloading of the
accessory offers the option of autoclave.
upgrading them with a more • Built in chromed iron and plastic.
resistant and higher quality
casters that include brakes. • The surface of each shelf is
textured to prevent the load
• Enables an easier movement of from shifting.
the equipment between different
locations. • Rubber coated wheels to reduce
noise.
• Must be installed in our facilities.
• Dimensions (LxDxH):
Reference: 4WHBR 730 x 490 x 700 mm.
Reference: TR-TR

CONDENSATES BOTTLE CABLE GLANDS

• 12L bottle with tap to collect • Installation of up to 8 cable


condensates during purge glands within the sterilization
phase and also to collect water chamber walls to enable
while cleaning the sterilization external temperature probe
chamber. Useful in situations access in multiple locations
where no easy drainage is for autoclave calibration and
available. validation procedures.
Reference: TANK-AE • These ports can either be of 2 or
4 mm of diameter.
References:
PRENSACLAV (8 holes ø 2mm),
PRENSACLAV2 (8 holes ø 4mm).

SW7000 SOFTWARE STERILIZATION CONTROL TAPE

• Communication software • Class 1 indicator for steam


between the equipment and sterilization. The change of color
the PC that allows the real-time indicates that the materials have
and posterior visualization and been processed, without being a
registry of each cycle. Cycles guarantee of proper sterilization,
can also be exported to Excel or additional methods are needed
printed. such as biological indicators
• Connection to PC via RS-232. (EN ISO 11138).
• Supplied with a RS-232 cable, • Tape roll of 50 m x 19 mm.
an USB stick that includes the Reference: TEST-CT
software and installation drivers
and a RS-232 to USB adapter. 20 min 121ºC
Color change.
Reference: SW7000

10 VERTICAL AUTOCLAVES WITHOUT DRYING - AES SERIES


TECHNICAL SUMMARY OF AES SERIES AUTOCLAVES
Recommended setting General laboratory
Equipment placement Floor-standing
General classification
Load direction Top-loading
Chamber profile Round
Liquids and culture media 

Laboratory waste bags 


Recommended type of load
Glassware 

Plastics 

Method to generate steam Heating elements


Sterilization technology features
Type of purge Gravity displacement
Transfer of data RS-232 

Integrated printer O
Batch printers
External printer O
Sterilization chamber volume 33 - 175 L
External building material AISI-304
Sterilization chamber material AISI-316L
Heating elements material Incoloy® 825
Gasket material Silicone rubber
Sterilization chamber and door
Min.- max. sterilization temperature 100 - 134°C
specifications
Maximum pressure (above atmospheric pressure) 2,1 Barg
Mechanism to open the door Manual wheel
Direction in which the door opens Lateral
Automatic locking with pressure 

Thermally insulated door 

Screen display Digital LCD


Screen size 2 lines x 16 digits
User interface and
Total number of available programs 10
microprocessor
Automatic microprocessor control 

Timer start 

Agar mode (temperature holding after cycle ends 40-80ºC) 

Special cycles and process Solids fast cooling



optimization (manual push-button for a faster cooling phase)
Temperature regulation by heart probe O
Agar mode 40 - 80ºC
Temperature of sterilization phase 100 - 134ºC
Adjustable cycle parameters
Duration of sterilization phase 1 - 250 min
Temperature regulation by heart probe On/Off
Multiple-use water sterilization chamber capacity 3 - 9,5 L
Flexible heart temperature probe O
Standard casters 

Other specifications Premium casters with brakes O


Pressure gauge 

Electric customization (115-230M V/230-400T V) O


Special models with augmented power O
Services Third-party qualification (IQ/OQ/PQ) O
: Standard O: Optional

VERTICAL AUTOCLAVES WITHOUT DRYING - AES SERIES 11


TECHNICAL DATA

Specifications
Reference AES-28 AES-50 AES-75 AES-110 AES-150
Total/usable chamber volume L 33/31 55/50 79/75 115/110 175/153
Usable chamber dimensions Ø x H mm 300 x 440 300 x 710 400 x 600 400 x 850 500 x 780
Sterilization chamber water tank volume L 3 3 5,5 5,5 9,5
Exterior dimensions L x D x H mm 505 x 580 x 1110 505 x 580 x 1290 610 x 700 x 1185 610 x 700 x 1435 750 x 820 x 1400
Loading height mm 795 975 870 1120 1085
Power W 2000 or 3200 3200 or 6000 3200 or 6000 4500, 6000 or 9000 6000 or 9000
Gross weight Kg 75 90 115 135 230
230V (1P+N) 16A
Voltage* V 230V (1P+N) 16A 230V (1P+N) 16A 400V (3P+N) 32A 400V (3P+N) 32A
or 400V (3P+N) 32A
Frequency Hz 50/60 50/60 50/60 50/60 50/60
*Other voltages available under request. Special models with augmented power may operate with other voltages.

Safety features General features


• Safety valve. Adjustable sterilization
100 - 134ºC
temperature
• Safety thermostat with manual rearm for the heating elements.
• Pneumatic door blocking system while positive pressure exists inside the Adjustable sterilization time 1 - 250 min
sterilization chamber.
• Open door sensor. Max. pressure 2,1 Barg
• Thermally insulated door. Fully automatic microprocessor control by either
• Heating elements cover. Sterilization control system chamber temperature probe or flexible heart
• Several visual and acoustic safety and warning alarms. temperature probe
Air purge system Gravity displacement

Regulations External building material AISI-304 stainless steel

All our AES Series autoclaves are designed to comply with the strictest Sterilization chamber material AISI-316L stainless steel
international directives and standards, including the following regulations: Heating elements material Incoloy® 825
• EN-61010-1 Safety requirements for electrical equipment for
Gasket material Silicone rubber
measurement, control and laboratory use. Part 1: General requirements.
• EN-61010-2-040 Part 2-040: Requirements for laboratory autoclaves. Connection to PC RS-232
• EN-61326 Electrical equipment for measurement, control and laboratory
Connection to printer RS-232 or integrated
use. EMC requirements.
• AD 2000 Merkblatt Pressure vessels. Number of programs 10 (4 preset and 6 user free)
• 2014/35/UE Low voltage. Programmable auto-start Up to 24 h
• 2014/30/UE Electromagnetic compatibility.
• 2014/68/UE Pressure equipment. Screen type LCD display

Opening door mode Horizontal swiveling door with blocking wheel

Self-control of obtained values (Tº & t)


Monitoring of sterilization vs programmed values. Cycle is automatically
parameters interrupted if obtained values differ from
CLICK! programmed values
ACCESS
THE AES Pressure display Pressure gauge on control panel
SERIES
VIDEO Water is directly poured into the sterilization
Water management
chamber
+ info

Drainage connection operated by an independent


Drainage system drainage valve on control panel for manual
release of sterilization chamber water tank
Included standard casters, optional upgrade to
Casters
premium casters with brakes
REV 06.2021

Find out more about our AES Series autoclaves on our Youtube
Channel

Installation guide available under request, please contact us.

Avinguda del Vallès, 322


Pol. Ind. “Els Bellots” [email protected]
08227 Terrassa (Barcelona) Spain www.raypa.com Tel. +34 937 830 720 R. ESPINAR, S.L.

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