PP603 Molecular approaches for improving physiological traits 2+1

Course leader: Dr. Viswanathan Chinnusamy

Course instructors: Dr. Pranjal Yadava & Dr. M. Nagaraj Kumar
Theory: Monday 10:15 AM & Friday 11:15 PM
Practical: Friday 2:15-5:00 pm
Date Topic Theory/ Instructor
Practical
1. 28-01-2022 Introduction to the Course T Dr. Viswanathan
Chinnusamy
2. 28-01-2022 Bioinformatics – sequence analysis, structure analysis P Dr. Pranjal Yadava
3. 29.01-2021 Phenotyping: Importance and approaches for gene T Dr. Viswanathan
mapping and molecular genetics. Chinnusamy
4. 31-01-2022 Transgenic approach in improving physiological T Dr. M. Nagaraj
processes- Introduction to Kumar
GMOs and application in crop improvement
5. 04-02-2022 The concept of molecular markers, various types of T Dr. Viswanathan
dominant and co-dominant marker systems Chinnusamy
6. 04-02-2022 Molecular biology - genomic/plasmid DNA isolation, P Dr. Pranjal Yadava
RNA isolation.
7. 14-02-2022 Concept of QTL mapping/GWAS & Strategies for QTL T Dr. Viswanathan
introgression and Marker Assisted Selection (MAS) Chinnusamy
8. 18-02-2022 Allele mining & its applications in crop improvement T Dr. Viswanathan
Chinnusamy
9. 18-02-2022 Molecular markers P Dr. Viswanathan
Chinnusamy
10. 19-02-2021 Genome editing approaches T Dr. Viswanathan
Chinnusamy
11. 21-02-2022 Application of genome editing for crop improvement T Dr. Viswanathan
Chinnusamy
12. 25-02-2022 Identification of candidate genes for various physiological T Dr. M. Nagaraj
process associated with specific traits (such as stress Kumar
tolerance) and their potential benefits in transgenic crops.
13. 25-02-2022 Full length and truncated gene cloning P Dr. M. Nagaraj
Kumar
14. 26-02-2022 Cloning: Principles and concepts T Dr. M. Nagaraj
Kumar
15. 28-02-2022 Gene mining, sequence T Dr. Pranjal Yadava
structure & function analysis using bioinformatics tools
16. 04-03-2022 Map based cloning of novel genes and alleles T Dr. Pranjal Yadava
17. 04-03-2022 Genotyping by using chip and sequencing P Dr. Pranjal Yadava
18. 05-03-2022 Mid-semester exam Dr. Viswanathan
Chinnusamy
19. 07-03-2022 Transformation of crop plants – Principles & Methods T Dr. M. Nagaraj
Kumar
20. 11-03-2022 Cloning strategies: promoters, gene stacking, functional T Dr. M. Nagaraj
validation Kumar
21. 11-03-2022 Vector construction with specific promoter, gene stacking P Dr. Pranjal Yadava
and transient assays
22. 12-03-2022 Approaches for gene cloning and characterization T Dr. Viswanathan
Chinnusamy
23. 14-03-2022 Selection and analysis of transformants T Dr. M. Nagaraj
Kumar
24. 21-03-2022 Functional Evaluation of transgenic plants by T Dr. M. Nagaraj
physiological conditions Kumar
25. 25-03-2022 Biosafety and Regulation of GMOs T Dr. Pranjal Yadava
26. 25-03-2022 Transformation in model system and in crops- P Dr. M. Nagaraj
agrobacterium mediated & particle gun Kumar
27. 28-03-2022 Registration of Transgenic Agricultural Organisms T Dr. Pranjal Yadava
28. 01-04-2022 Methods to detect GMOs from agricultural products T Dr. Pranjal Yadava
29. 01-04-2022 Design of gRNA & Sequence analysis of genome edited P Dr. Viswanathan
plants Chinnusamy
30. 04-04-2022 Molecular approaches for improving nutrient use T Dr. Pranjal Yadava
efficiency- Case studies
31. 08-04-2022 Molecular approaches for improving Yield- Case studies T Dr. Pranjal Yadava
32. 08-04-2022 Molecular analysis of genome edited plants P Dr. Viswanathan
Chinnusamy
33. 11-04-2022 Molecular approaches for improving water use efficiency- T Dr. Viswanathan
Case studies Chinnusamy
34. 18-04-2022 Molecular approaches for improving Flood tolerance- Case T Dr. Pranjal Yadava
studies
35. 22-04-2022 Molecular approaches for improving growth and T Dr. M. Nagaraj
development- Case studies Kumar
36. 22-04-2022 Evaluation of transgenics – semi quantitative and P Dr. M. Nagaraj
quantitative RT-PCR Kumar
37. 25-04-2022 Molecular approaches for improving combined stress T Dr. M. Nagaraj
tolerance- Case studies Kumar
38. 29-04-2022 Rapid Generation Advancement and its application T Dr. Viswanathan
Chinnusamy
39. 29-04-2022 Southern, northern, western blotting, ELISA P Dr. M. Nagaraj
Kumar
40. 02-05-2022 Small RNA Analysis T Dr. Pranjal Yadava
41. 06-05-2022 Epigenome and epialleles for crop improvement T Dr. Viswanathan
Chinnusamy
42. 06-05-2022 Functional assays for gene validation- Physiological assays P Dr. M. Nagaraj
Kumar
43. 07-05-2021 Functional assays for gene validation- Biochemical assays P Dr. M. Nagaraj
Kumar
44. 09-05-2022 Assignment – Presentation by students Dr. M. Nagaraj
Kumar & Dr.
Pranjal Yadava
45. 13-05-2022 Assignment – Presentation by students Dr. M. Nagaraj
Kumar & Dr.
Pranjal Yadava
46. 13-05-2022 Practical exam
47. 20-05-2022 Final Theory exam