Volume 7, Issue 8, August – 2022 International Journal of Innovative Science and Research Technology

ISSN No:-2456-2165

Formulation and Evaluation of Herbal Chocolates by

using Withania Somnifera (Ashwagandha) and
Asparagus Racemosus (Shatavari).
Kaushik Singh1, Komal Shevkar 2, Omkar Selmokar 3, Prof. Poonam Harpale4, Dr. Rajesh Oswal5
Research Scholor1,2,3 B. Pharmacy Final Year, Assistant Professor Department of Pharmaceutics4, Principal5
Genba Sopanrao Moze college of Pharmacy, Wagholi, Pune 412207

Abstract:- The chocolate is most loving food among the outstandingly as a nervine tonic. Ashwagandha comprises of
people whereas the medicine is hating substance. So, dried mature underlying foundations of Withania somnifera
objective of present study was to formulate the chocolate Donal. (Fam. Solanaceae), a perpetual bush, tracked down in
that contain drug i.e., medicated chocolate to prevent squander land, developed field and open grounds all through
various kind of disorder. Withania somnifera India.
(Ashwagandha) is an herbal drug which having several
medicinal properties like Anti stress, anti-inflammatory  Chemical constituents:
effect and nervous system effect and Asparagus Withania somnifera contain of 35 synthetic substances,
racemosus (Shatavari) is the herbal drug which having withanine is the super constituent present. It contains
medicinal properties, like prevents miscarriage, increases alkaloids and withanolides, for example, somniferine,
lactation, removes the infertility, and regulates the somnine, somniferinine, withananine, pseudo-withanine,
menstruation. Thus, we must formulate the chocolate tropine, pseudo-tropine and withaferin A.
with powder of Ashwagandha and Shatavari that gives
the desired pharmacological effect. Further, prepared  Therapeutic effect
medicated chocolate is evaluated for general appearance,  Antistress Effect
dimension, hardness, blooming test. Ashwagandha is customarily utilized as an adaptogen
which is useful in lessening pressure and uneasiness.
Keywords:- Herbal chocolate, Withania somnifera Ashwagandha seems to assist with controlling go betweens of
(Ashwagandha), Asparagus racemosus (Shatavari), stress, including heat shock proteins (Hsp70), cortisol, and
pharmacological effects. stress-enacted c-Jun N-terminal protein kinase (JNK-1) (4).
Anti-inflammatory Properties.
I. INTRODUCTION
Ashwagandha root extricate applies calming and anti-
A. General Introduction To Chocolate Drug Delivery System inflammatory impacts in HaCaT cells by repressing the
Perhaps of the most famous food all around the world is MAPK/NF κB pathways and by controlling cytokines. Debris
chocolate and it has profoundly nutritious energy, quick WEX essentially hindered mRNA articulation of provocative
digestion, and great absorbability. When consumed as a cytokines, including interleukin (IL) 8, IL 6, cancer
component of a reasonable and changed diet, chocolate can putrefaction factor (TNF α), IL 1β and IL 12, and advanced
be both a wellspring of supplements too as joy, and can the mRNA articulation of the calming cytokine changing
considered as be important for an invigorating, healthy eating development factor (TGF) β1 in HaCaT cells.
regimen. Cured chocolate detailing is generally utilized for
organization and it builds the patient's longing to consume the  Antitumor Properties
medicine. Sedated chocolate is ready by utilizing chocolate WS and its tamanolide’s been displayed to have
base and medication is integrated to arranged chocolate base. remedial potential against disease, some of them have
The sedated chocolate can be assessed for its appearance, additionally been displayed to have malignant growth
dampness content, thickness, blossoming test. Cured preventive properties. The disease battling properties have
chocolate is ready by utilizing chocolate base and the been seen with root extricates, yet in addition with leaf
medication is integrated into arranged chocolate base. As the separates which is a generally underused a piece of the
medication is consolidated inside the chocolate and the Ashwagandha plant.
medication is let out of the chocolate, it is called as Chocolate
medication drug delivery system.  Immunomodulatory
The ashwagandha root separate contained withanolides,
B. Introduction To Drug Introduction To Withania for example, withaferin A, withanolide An and withanolide
Somnifera (Ashwagandha) sulfoxide, which have solid immunomodulatory effect.
Withania somnifera (Ashwagandha) is a profoundly
respected spice in the Indian Ayurvedic clinical framework
(tonic). It is utilized for various infirmities, however most

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Volume 7, Issue 8, August – 2022 International Journal of Innovative Science and Research Technology
ISSN No:-2456-2165
 Antioxidant Effect  Therapeutic Effects
Ashwagandha (Withania somnifera) water extricate is a  Galactogogue effect
strong cancer prevention agent and has anticancer properties The root extract of A. racemosus is used to increases
in HepG2 cells milk secretion during laction.

 Nervous System Effects  Antisecretory and antiulcer activity

It is utilized for different sorts of illness processes and Shatavari was found to remember the greater part of the
exceptionally as a nervine tonic. It has a Cognition Promoting side effects in larger part of the patients. The ulcer
Effect and is helpful in youngsters with memory deficiency recuperating impact of the medication was credited to a direct
and in advanced age individual loss of memory. It is helpful to an immediate mending impact, conceivable by potentiating
in neurodegenerative illnesses like Parkinson's, Huntington’s, inherent defensive element as it has neither antisecretory
and Alzheimer's sicknesses. It has GABA mimetic impact and movement nor stomach settling agent properties, by
was displayed to advance development of dendrites. It makes reinforcing mucosal opposition, drawing out the life
anxiolytic difference and further develops energy levels and expectancy of mucosal cells, expanding emission and
mitochondrial wellbeing. consistency of mucous and lessening H+ particle back
dissemination
 Effects on the Cardiopulmonary System
Ashwagandha-arranged as Rasayanas, and portrayed to  Antitussive effect
advance wellbeing and life span and Arjuna basically for Methanolic extract of roots showed huge antitussive
heart infirmities. coronary conduit illness, cardiovascular action. Shatavari diminishes the discharge of cough.
breakdown, hypercholesterolemia, anginal torment and can
be considered as a helpful medication for coronary corridor  Antiprotozoal activity
infection, hypertension, and ischemic cardiomyopathy. A fluid arrangement of the unrefined alcoholic
Ashwagandha demonstrate helpful to improve cardio- concentrate of the roots displayed an inhibitory impact of the
vascular perseverance and bringing down systolic circulatory development of Eintamoeba histolytica.
strain.
 Effect on uterus
C. Introduction To Asparagus Racemosus (Shatavari) Asparagus racemosus is notable for its consequences for
Asparagus racemosus is a native restorative plant of the the female conceptive framework and utilized in all female
family Liliaceae is significant for its sapogenin content the related issues like PMS. It likewise upholds further tissue and
antecedent of numerous pharmacologically dynamic steroids. fabricates blood thus it assists with eliminating fruitlessness,
It has cancer prevention agent action, hostile to abortifacient set up the belly for origination, forestalls unsuccessful labor
movement Shatvarin Antioxytoxic (shatavarin4), convulsive and goes about as a post pregnancy tonic where it assists with
to uterus, hypoglycemic, hypertensive action, anticoagulant expanding lactation and standardize the uterus, prolapse of
action, antiviral action, anticancer, antidysenteric action. Uterus and the adjusting regenerative chemicals level.

 Chemical Constituents  Toxic effect

Steroidal saponins, known as savarins. Savarins I to VI A. racemosus has been descrived as totally ok for long
are available. Savarino I is the significant glycoside with 3- haul use, in any event, during pregnancy and lactation.
glucose and rhamnose moieties connected to sarsapogenin. Foundational organization of higher portions of l concentrates
Oligospirostanoside alluded to as Immunoside. Polycyclic did not deliver just abnormity.
alkaloid-Aspargamine A, an enclosure type pyrrolizidine
alkaloid. Isoflavones-8-methoxy-5, 6, 4-trihydroxy  Other therapeutic effect: -
isoflavone-7-0-beta-D-glucopyranoside. Cyclic  Uterine tonic
hydrocarbon-racemosol, dihydrophenantherene. Furan  Used in PMS
compound-Racemofuran. Carbs Polysacharides, adhesive.  Removes infertility by supporting deeper tissue and builds
Flavanoids-Glycosides of quercitin, rutin and hyperoside are blood
available in blossom and natural products, Sterols-Roots  Prevents Miscarriage
additionally contain sitosterol, 4, 6-dihydryxy 2-O (- 2-  Increases lactation
hydroxy isobutyl) benzaldehyde and undecanyl cetanoate  Regulates menstruation
 Improve conception rate
 Balance reproductive hormones

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Volume 7, Issue 8, August – 2022 International Journal of Innovative Science and Research Technology
ISSN No:-2456-2165
II. MATERIALS AND METHODS

A. Materials:

Sr. no Ingredients
1 Dark chocolate
2 White compound
3 Ashwagandha
4 Shatavari
5 Sugar
Table (1): - List of ingredients

B. Method of preparation:

A. Method of preparation of chocolate by using Ashwagandha

Content F1 F2 F3 F4 F5
Dark chocolate 3gm 3gm 3.5gm 3.5gm 3.5gm
White chocolate 3.5gm 3.5gm 3gm 3gm 3gm
Sugar 2gm 1gm 2gm 2gm 3gm
Ashwagandha 0.45gm 0.5gm 0.55gm 0.6gm 0.65gm
Table (2): - Composition formulation of Ashwagandha

Water bath was set in such a way that water become hot melted chocolate base. After above step, appropriate quantity
having temperature about 50℃. Then chocolate base was of drug extract i.e., crude extract of Ashwagandha 450mg,
melted in porcelain dish till it become free flowing. On 500mg ,550mg, 600mg, 650mg were added to it and stirred
another side, sugar syrup was prepared by taking sugar in continuously. Then whole mass of chocolate base was poured
appropriate quantity with distilled water in beaker on water in a silicon chocolate mould and refrigerated till it become
bath. Then add prepared sugar syrup in required quantity into solid form approximate 3-6Hrs.

Fig (1): - Mixture of chocolate compound

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Volume 7, Issue 8, August – 2022 International Journal of Innovative Science and Research Technology
ISSN No:-2456-2165
B. Method of Preparation of chocolate

Fig (2): - Ashwagandha chocolate mould

C. Method of preparation of chocolate by using Asparagus racemosus (Shatavari)

Content F1 F2 F3 F4 F5
Dark chocolate 3gm 3.5gm 3gm 3gm 3.5gm
White chocolate 3.5gm 3gm 3.5gm 3.5gm 3gm
Sugar 2gm 1gm 2gm 2gm 3gm
Shatavari 0.45gm 0.5gm 0.55gm 0.6gm 0.65gm
Table (3): - Composition formulation of Shatavari

Water bath was set in such a way that water become hot melted chocolate base. After above step, appropriate quantity
having temperature about 50℃. Then chocolate base was of drug extract i.e., crude extract of Chiavari 450mg, 500mg
melted in porcelain dish till it become free flowing. On ,550mg, 600mg, 650mg were added to it and stirred
another side, sugar syrup was prepared by taking sugar in continuously. Then whole mass of chocolate base was poured
appropriate quantity with distilled water in beaker on water in a silicon chocolate mould and refrigerated till it become
bath. Then add prepared sugar syrup in required quantity into solid form approximate 3-6Hrs.

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Volume 7, Issue 8, August – 2022 International Journal of Innovative Science and Research Technology
ISSN No:-2456-2165
D. Method of Preparation of Chocolate

Fig (3): - Shatavari chocolate mould

III. EVALUATION D. Blooming test

Fat Bloom - At the point when the slight layer of fat
A. General Appearance precious stones structure on the outer layer of chocolate plan.
Colour This will make the chocolate lose its sparkle and a delicate
Odour white layer will show up, giving the completed article an
Taste unappetizing look. Fat blossom is brought about by the
Texture recrystallization of fat or potentially a movement of a filling
fat to the chocolate layer. Capacity at a consistent temperature
B. Dimensions will defer the presence of fat sprout.
It was measured by Vernier’s callipers.
Sugar Bloom – This is rough and irregular layer on top
C. Hardness Test of chocolate formulation. This is caused by condensation
Hardness of chocolate was measured by Monsanto (when chocolate is taken out of the refrigerator). This
Hardness Tester moisture will dissolve the sugar in the chocolate. When the
water evaporates, sugar recrystallizes into rough, irregular
crystals on surface. This results into unpleasant look.

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Volume 7, Issue 8, August – 2022 International Journal of Innovative Science and Research Technology
ISSN No:-2456-2165
IV. RESULT AND DISCUSSION

A. Result for Ashwagandha: -

 Organoleptic evaluation: -

Sr.no Parameters Observation

1 Colour Dark Brown
2 Odour Chocolate with no brunt, no smoky smell
3 Taste sweet
4 Texture Smooth and even
Table (4): - Organoleptic evaluation parameter for Ashwagandha

 Dimensions: -
Height: - 1.3 cm
Diameter: - 2.7 cm

 Hardness test: -

Content (Ashwagandha) Result (Hardness)

F1 10.2 ±2 kg/cm2
F2 10.45 ±2 kg/cm2
F3 10.6 ±2 kg/cm2
F4 10.5 ±2 kg/cm2
F5 10.45 ±2 kg/cm2
Table (5): - Hardness Parameter for Ashwagandha

 Blooming test: -

Test Result
Fat Bloom No
Sugar Bloom No
Table (6): - Blooming test parameter for Ashwagandha

B. Results For Shatavari

 Organoleptic evaluation: -

Sr.no Parameters Observation

1 Colour Dark Brown
2 Odour Chocolate with no brunt, no smoky smell
3 Taste sweet
4 Texture Smooth and even
Table (7): - Organoleptic evaluation parameter for Shatavari

 Dimensions
Height: - 1.3 cm
Diameter: - 2.8 cm

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Volume 7, Issue 8, August – 2022 International Journal of Innovative Science and Research Technology
ISSN No:-2456-2165
 Hardness test: -

Content (Shatavari) Result (hardness)

F1 13.6 ±2 kg/cm2
F2 13.8 ±2 kg/cm2
F3 13.6 ±2 kg/cm2
F4 13.5 ± 2 kg/cm2
F5 13.65 ±2 kg/cm2
Table (8): - Hardness Parameter for Shatavari

 Blooming test

Test Result
Fat Bloom No
Sugar Bloom No
Table (9): - Blooming test parameter for Shatavari

V. CONCLUSION [7]. Kumar, S., Mehla, R. K., & Dang, A. K. (2008). Use
of shatavari (Asparagus racemosus) as a
In the current review advancement of natural chocolate galactopoietic and therapeutic herb–a
by utilizing Ashwagandha and Shatavari was done. By review. Agricultural Reviews, 29(2), 132-138.
utilizing arranged powder of Ashwagandha and Shatavari [8]. Sikandan A, Shinomiya T, Nagahara Y. Ashwagandha
home grown chocolates was ready and assessed for outward root extract exerts anti-inflammatory effects in HaCaT
presentation, aspect, hardness, blossoming test. cells by inhibiting the MAPK/NF-κB pathways and by
regulating cytokines. Int J Mol Med. 2018
From above concentrate on we presumed that the Jul;42(1):425-434. doi: 10.3892/ijmm.2018.3608.
chocolates give smooth and velvety surface to the detailing Epub 2018 Apr 2. PMID: 29620265.
and are great for covering the horrendous taste related with [9]. Palliyaguru, D. L., Singh, S. V., & Kensler, T. W.
the medications and the medication which are utilized in the (2016). Withania somnifera: From prevention to
portion range are protected and can be taken with no gamble treatment of cancer. Molecular nutrition & food
of aftereffects. research, 60(6), 1342–1353.
[10]. Ahmed, W., Mofed, D., Zekri, A. R., El-Sayed, N.,
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