BIOTECHNOLOGY
CATEGORY L T P CREDIT
BTT312 ANIMAL & PLANT CELL TECHNOLOGY
PEC 2 1 0 3
Preamble: Required to know the structure and function of plant and animal cell
Prerequisite:Knowledge of fundamentals in plant and animal cell biology
Course Outcomes: After the completion of the course the student will be able to
Disseminate and inculcate knowledge of various plant and animal tissue culture
CO 1 techniques.
CO 2 Analyse different medias for cell culture.
CO 3 Integrate biological and engineering principles in gene transfer methods
CO 4 Appraise the aspectsof various products obtained through cell culture technologies.
Mapping of course outcomes with program outcomes
PO 1 PO 2 PO 3 PO 4 PO 5 PO 6 PO 7 PO 8 PO 9 PO PO PO
10 11 12
CO 1 - - - - - 2 3 2 - - - 3
CO 2 - - - - - 2 3 2 - - - 3
CO 3 - - - - - 2 3 2 - - - 3
CO 4 - - - - - 2 3 2 - - - 3
Assessment Pattern
Bloom’s Category Continuous Assessment Tests End Semester Examination
1 2
Remember 10 10 10
Understand 20 20 20
Apply 20 20 70
Analyse
Evaluate
Create
Mark distribution
Total Marks CIE ESE ESE Duration
150 50 100 3 hours
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Continuous Internal Evaluation Pattern:
Attendance : 10 marks
Continuous Assessment Test (2 numbers) : 25 marks
Assignment/Quiz/Course project : 15 marks
End Semester Examination Pattern: There will be two parts; Part A and Part B. Part A
contain 10 questions with 2 questions from each module, having 3 marks for each question.
Students should answer all questions. Part B contains 2 questions from each module of
which student should answer any one. Each question can have maximum 2 sub-divisions
and carry 14 marks.
Course Level Assessment Questions
Course Outcome 1 (CO1): Disseminate and inculcate knowledge of various plant and animal
tissue culture techniques
1. State the applications of micropropagation.
2. Give the details about the herbicide tolerant plants.
3. Explain the concept of somaclonal variation.
Course Outcome 2 (CO2): Analyse different medias for cell culture
1. Justify the role of serum in cell culture techniques.
2. Give the details about the protoplast technology.
3. Recall the role of carbon dioxide in animal cell culture.
Course Outcome 3(CO3):Integrate biological and engineering principles in gene transfer
methods
1. Explain the process of transgenic plantproduction.
2. Explain the process of coat protein mediated resistance.
3. Describe the embryonic stem cells and their applications.
Course Outcome 4 (CO4): Appraise the aspectsof various products obtained through cell
culture technologies
1. Demonstrate the production of cell-culture based vaccine.
2. Give the details about the herbicide tolerant plants.
3. Describe the process of embryo rescue
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Model Question Paper
Total Pages:
Reg No.:______________ Name:________________________
APJ ABDUL KALAM TECHNOLOGICAL UNIVERSITY
SIXTH SEMESTER B. TECH DEGREE EXAMINATION ________ ____ 20__
Course Code: BTT312
Course Name: ANIMAL & PLANT CELL TECHNOLOGY
Max. Marks: 100 Duration: 3 Hours
PART A
Answer all questions, each carries 3 marks.
1 a) What are the different sources of serum for animal cell culture?
b) Explain the significance of embryonic stem cells.
c) Explain the role of carbon dioxide in cell culture.
d) What is the significance of cell bank?
e) List out the applications of biodiesel
f) Name three microorganisms used for bioethanol production.
g) Give a note on Butanol fuel mixtures
h) What is the common feed stock materials used in biogas production?
i) Give a note on auto hydrolysis.
j) Brief about cellulose saccharification
PART B
Answer any one full question from each module. Each carries 14 marks.
2 a) Explain the chemical, physical and metabolic functions of different (14)
constituents of culture medium.
OR
3 a) Justify the role of carbon dioxide in animal cell culture (7)
b) Describe the role of BSS (7)
4 a) Elaborate the methods used for measurement of viability of cell culture. (14)
OR
5 a) What are the methods for Identification of specific cell lines? (7)
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b) Explain the role of collagenase in tissue disaggregation. (7)
6 Explain the methods for production of vaccines. (14)
OR
7 a) Elaborate the significance of embryonic stem cells. (8)
b) Justify the role of animals as bioreactors (6)
8 Explain the role of protoplast technology in crop improvement. (14)
OR
9 a) a) What are pathogen free plants (7)
b) b) Explain the significance of somaclonal variation. (7)
10 Explain CPMR in detail. (14)
OR
11 Elaborate the role of plasmids in crop improvement (14)
****
Syllabus
Module 1
Introduction to animal tissue culture: Laboratory design, aseptic conditions, methodology
and media; balanced salt solution and simple growth medium. Brief discussion on the
chemical, physical and metabolic functions of different constituents of culture medium. Role
of carbon dioxide. Role of serum and supplements. Serum & protein free defined media and
their applications; Equipment and materials for animal cell culture technology
Module 2
Cell culture: Basic techniques of mammalian cell culture in vitro; disaggregation of tissue,
primary culture establishment; maintenance of cell culture. Cell lines- characteristics and
routine maintenance. Measurement of viability and cytotoxicity. Cultured cells- Biology and
characterization: Characteristics of cultured cells, Cell bank, Measurement of growth
parameters of cultured cells, Cell adhesion, Cell proliferation and differentiation,
Identification of specific cell lines
Module 3
Biotechnological applications in animal improvement: Organ and histotypic cultures, three-
dimensional culture - Tissue engineering, applications of animal cell culture- Stem cell
cultures, embryonic stem cells and their applications. Cell culture-based
vaccines.Improvements of animals using transgenic approach with specific examples,
animals as bioreactors. Monoclonal antibodies and their commercial production.
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Module 4
Plant tissue culture: History of plant tissue culture, aseptic conditions, methodology, media.
Different types of culture- callus cultures, meristem cultures, anther culture, embryo
culture, micro propagation, protoplast culture, somaclonal variation, synthetic seeds.
Methods of plant tissue preservation and applications
Module 5
Gene Transfer methods: Agrobacterium tumefaciens mediated transfer- techniques of
transferring agronomically important genes using Ti plasmid, Ri plasmid. Methods of direct
gene transfer. Production of transgenic plants. Development of herbicide resistance-
glyphosate, phospinothricin, sulfonyl urea, atrazine resistant transgenic plants. Virus
resistance: Coat protein mediated resistance. Cell suspension cultures and bioreactor
technology, secondary metabolites, Plant-derived vaccines, plant bodies. Purification of
plant products and its applications. Germplasm conservation
Text Books
1. H S Chawla, Introduction to Plant Biotechnology, Science Publishers, 2002
2. Basic Cell Culture: A Practical Approach. J.M. Davis, Oxford University Press, oxford.
3. Culture of Animal Cells: A Manual of Basic Technique. R. Ian Freshney Wiley-Liss.
4. Hammond J, McGarvey P, Yusibov V (Eds), Plant Biotechnology -New Products and
Applications, Springer, 1999.
5. Tong-Jen Fu, Gurmeet Singh, Wayne R. Curtis (Eds), Plant Cell and Tissue Culture for
the production of Food Ingredients, Springer Science & Business Media, 1999.
Reference Books
1. Rian Freshney, Culture of Animal Cells: A Manual of Basic Technique and Specialized
Applications, 6/e, Wiley-Blackwell, 2010.
2. John R W Masters (Ed.), Animal Cell Culture - A Practical Approach, 3/e, Oxford
University Press, 2000.
3. Jackson JF, Linskens HF (Eds.), Genetic Transformation of Plants, Springer, 2003.
4. M K Razdan, Introduction to Plant tissue culture, Science Publishers, 2003.
5. Gene VII. B. Lewin. Oxford University Press, New York
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Course Contents and Lecture Schedule
No Topic No. of
Lectures
1 Introduction to animal tissue culture
1.1 Laboratory design, aseptic conditions, methodology and media;
Balanced salt solution and simple growth medium 3
1.2 Brief discussion on the chemical, physical and metabolic functions of
different constituents of culture medium.
1.3 Role of carbon dioxide. Role of serum and supplements 1
1.4 Serum & protein free defined media and their applications
1.5 Equipment and materials for animal cell culture technology 3
2 Cell culture
2.1 Basic techniques of mammalian cell culture in vitro; disaggregation of 2
tissue
2.2 Primary culture establishment; maintenance of cell culture. Cell lines- 2
characteristics and routine maintenance. Measurement of viability
and cytotoxicity.
2.3 Cultured cells- Biology and characterization: Characteristics of
cultured cells, Cell bank 1
2.4 Measurement of growth parameters of cultured cells , Cell adhesion
2.5 Cell proliferation and differentiation, Identification of specific cell lines 1
3 Biotechnological applications in animal improvement
3.1 Organ and histotypic cultures,Three-dimensional culture - Tissue 2
engineering, applications of animal cell culture
3.2 Stem cell cultures, embryonic stem cells and their applications. Cell 2
culture-based vaccines
3.3 Improvements of animals using transgenic approach with specific 2
examples
3.4 Animals as bioreactors. Monoclonal antibodies and their commercial 2
production.
4 Plant tissue culture
4.1 History of plant tissue culture, aseptic conditions, methodology, 1
media, techniques of callus cultures
4.2 Meristem cultures, anther culture, embryo culture, micropropagation 2
4.3 Protoplast culture, somaclonal variation, synthetic seeds 2
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4.4 Methods of plant tissue preservation and applications 1
5 Gene Transfer methods
5.1 Agrobacterium tumefaciens mediated transfer- techniques of 2
transferring agronomically important genes using Ti plasmid, Ri
plasmid
5.2 Methods of direct gene transfer. Production of transgenic plants. 1
5.3 Development of herbicide resistance- glyphosate, phospinothricin, 2
sulfonyl urea, atrazine resistant transgenic plants.
5.4 Virus resistance: Coat protein mediated resistance, Cell suspension 1
cultures and bioreactor technology
5.5 Secondary metabolites, Plant-derived vaccines, plant bodies 1
5.6 Purification of plant products and its applications, Germplasm 1
conservation
