Biological Safety Cabinet

Class II A2
BSC-1100IIA2-X

BSC-1300IIA2-X

BSC-1500IIA2-X

USER MANUAL

Jinan Biobase Biotech Co., Ltd

Version 2019.05
 Preface
Thank you very much for purchasing our class II A2 biological safety cabinet.
Please read the “Operating Instructions” and “Warranty” before operating this unit to assure proper
operation. After reading these documents, be sure to store them securely together with the
“Warranty” within touch for future reference.

Warning: Before operating the unit, be sure to read carefully and fully understand important
warnings in the operating instructions.
Disclaimer
Biobase shall not be liable for any equipment failure or damage, or for any direct or indirect damage
that may occur during the use of the equipment.
1.Malfunction or damage due to violation of the instructions, precautions, and intended use of this
manual.
2.Malfunction or damage caused by repair or alteration of the other company.
3.Malfunction or damage caused by use instruments of other company at the same time .
4.Malfunction or damage caused by operating environment not corresponding to the specified
operating environment (power conditions, installation environment, etc).
5.Malfunction or damage caused by natural disasters such as earthquakes and floods.
6.Malfunction or damage caused by the company unaware of the movement or transfer (transport)
after installation.
Contact information
Company: Jinan Biobase Biotech Co., Ltd
Company Address: NO. 51 South Gongye Road, Jinan, China
Service Email: [email protected]
Fax: +86-531-81219804
Tel: +86-531-81219801/03
Zip code: 250000

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 CONTENT

1. Unpacking, Installation, Debugging............................................................................................... 5

1.1 Unpacking.....................................................................................................................................5
1.2 Accessories checking.................................................................................................................... 7
1.3 Installation conditions and using environment............................................................................. 8
1.4 Installation.................................................................................................................................... 8
1.5 Checking after installation.......................................................................................................... 13

2. User Instructions............................................................................................................................ 14
2.1 Functions.....................................................................................................................................14
2.1.1 Product Concept................................................................................................................... 14
2.1.2 Application Range................................................................................................................ 14
2.1.3 Working theory/Air flow pattern and protected area............................................................14
2.1.4 Protected objects...................................................................................................................15
2.1.5 TECHNICAL PARAMETERS............................................................................................ 15
2.1.6 Performance Index............................................................................................................... 16
2.2 Product structure......................................................................................................................... 17
2.2.1 Structural composition of BSC-1100IIA2-X........................................................................17
2.2.2 Structural composition of BSC-1300IIA2-X....................................................................... 18
2.2.3 Structural composition of BSC-1500IIA2-X....................................................................... 19
2.2.4 Structure introduction........................................................................................................20
2.3 Control panel...............................................................................................................................22
2.4 Remote Control &Foot switch....................................................................................................23
2.4.1 Remote control..................................................................................................................... 23
2.4.2 The use of foot switch.......................................................................................................... 25
2.5 Instructions for Operation...........................................................................................................25
2.5.1 Normal Operation Notice..................................................................................................... 25
2.5.2 Operation Process.................................................................................................................26
2.6. Daily maintenance..................................................................................................................... 27
2.6.1 Preparations before maintenance..........................................................................................27

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 2.6.2 Clean the cabinet surface......................................................................................................27
2.6.3 Overall maintenance period...............................................................................................27
2.6.4 Maintenance methods...........................................................................................................27
2.6.5 Storage conditions................................................................................................................ 28
2.7 Methods and procedures for disinfection....................................................................................29
2.8 Replacement parts list.................................................................................................................31
2.9 Wiring diagram........................................................................................................................... 34

3. Trouble shooting and Labels......................................................................................................... 36

3.1 Common faults & solution..........................................................................................................36
3.1.1 Warning and reminder.......................................................................................................... 36
3.1.2 Trouble shooting...................................................................................................................36
3.1.3 Simple accessories replacement........................................................................................... 38
3.2 Label Description........................................................................................................................41

4. Warranty......................................................................................................................................... 43

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1. Unpacking, Installation, Debugging
Please firstly check if packing box is in good condition. If the packing box is damaged, please take
photos.

1.1 Unpacking

1) Method 1: Use M8 Wrench to unpack

Picture 1

2) Method 2 Use M8 Wrench to unpack

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 Picture 2
Rapid unpacking diagram (Picture 3). Disassemble the screws shown in the below Picture, then
move the wooden pieces to right and left.

Picture 3

Rapid unpacking for BOX2

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Using ordinary scissors to cut packing tape, take off the package cover, then move up the paper box
body.

1.2 Accessories checking

Refer to the packing list and check the accessories.

Packing list (BSC-1100IIA2-X/BSC-1300IIA2-X/BSC-1500IIA2-X)
Items Model Position Quantity
Main Body Wooden box 1unit
BSC-1100IIA2-X 1pc
UV Lamp (T6 30W) Top or back of main
BSC-1300IIA2-X body 1pc

UV Lamp (T6 40W) BSC-1500IIA2-X 1pc

Base Stand Placed in two carton 1set

BSC-1100IIA2-X boxe 1pc
Fuse (10A) BSC-1300IIA2-X 1pc
BSC-1500IIA2-X 2pcs
Fuse (5A) 1pc
Placed in a transparent
Remote control (including battery) 1pc
plastic bag
Keys 2pc
User manual 1pc
Test report 1pc
Quality certification card 1pc
Power cord 1pc
Foot switch 1pc
Drain Valve 1set
Stainless steel hex cylinder head bolt 3pcs
M10×55
Stainless steel flat washer10 3pcs
Stainless steel spring washer 10 3pcs
Inner hexagon wrench 1pc
Big rubber gasket 1pc
Small rubber gasket 1pc
Front window tubular motor control rod 1pc
Buckle stopper (white) 7pcs

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1.3 Installation conditions and using environment

To avoid disturbances to the safety cabinet and its operator, follow the following guidelines, while
determining a suitable location for the cabinet:
a. The distance from the plane of the aperture to any circulation space should be at least 1000 mm,
so as to preserve a zone undisturbed by anyone other than the operator.
b. Biological safety cabinets should be placed in a position where there should be no opposing wall
(or other obstruction likely to affect the airflow) within 2000 mm of the front aperture.
c. Safety cabinets should not be installed in positions where they are likely to be affected by other
items or equipment. In particular the distance to the aperture of an opposing safety cabinet, fume
cupboard, or the edge of a local exhaust ventilation outlet should not be less than 3000 mm.
d. Any room air supply diffuser should not be within 1500 mm of the front aperture.
e. Doorways should not be within 1500 mm of the aperture or within 1000 mm of the side of the
safety cabinet.
f. The position of a safety cabinet should satisfy the spatial requirements (e.g. vision, lighting and
convenience of access) of the operator and personnel working nearby. When a cabinet is installed
on a bench top, the leading edge should be flush with or slightly overhanging the edge of the
bench top.
Working environment:
(1) Only is suitable for indoor;
(2) Ambient temperature: 15℃～35℃;
(3) Relative Humidity: ≤75%;
(4) Atmospheric pressure range: 70 kPa～106 kPa;
(5) Electrical parameters: Consistent with the rated voltage of the biosafety cabinet (See 2.1.5
technical parameter performance index);
(6) Power supply need to be grounded; (Judging method: testing the fire wire and the zero line of the
power supply with multimeter, the fire wire to ground voltage should be grid voltage and the zero
line to ground voltage should be 0, otherwise the power supply ground is bad).
(7) Test the voltage stability before using, if the voltage is unstable, should use the voltage regulator,
otherwise the control panel and transformer may be easily damaged.

1.4 Installation

a. Remove all the package materials;

b. Inspect the surface of main body to make sure whether there is scratch, deformation or
uncorrelated things;

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c. Move the whole device to the final installation location;

The base stand will be packed at back of main body, please take it out before installation.

DO NOT INVERT, DISASSEMBLE OR TITILE THE CABINET during transportation.

d. The base stand assembly

Referring to Picture 4 assemble the base stand.

Picture 4
Remove the M10×55 Hexagon socket head screws, Buckle plug, M10 Stainless steel flat washer,
M10 Stainless steel spring washer, M10 Stainless steel cap nuts, referring to Picture 4 assembled
base, firmly fastening requirements.

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e. Connect base stand and main body
Refer to Picture 5, 6 to connect base stand and main body.

Picture 5
Align the mounting holes on the bottom of the cabinet with the mounting bolts, and the cabinet
slowly descending the mounting base

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 Picture 6
Take out the M10*55 Hexagon socket head screws, Flat washer 10, Spring washer 10 from the
accessory box, and fasten tightly according to Picture 6.

f. Installation of Drain valve

Picture 7

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1. Drain valve connect
2. Shim (Inner diameter*outer diameter*thicknessΦ20*Φ28*2mm)
3. Safety cabinet bottom installation holes
4. Ball coupling fastening nut
5. Rubber gasket (Inner diameter*outer diameter*thicknessΦ13*Φ19*2mm)
6. Drain valve
Take out drain valve coupling, shim, Ball nut, Rubber gasket, Drain valve, assembling from up to
down as Picture 7 illustrated.
g. Adjustment of Footmaster Caster

Picture 8
Clockwise rotate caster’ red part to low down the base feet and the height of the cabinet. Low down
all four casters can move the cabinet position. Counterclockwise rotate casters’ red part can rise the
base leg and height of cabinet. Raise all four casters can at same time can fix the cabinet. Adjust the
four Foot -masters makes the cabinet stable.
h. Foot Switch

Picture 9
Install Foot Switch as Picture 9. It’s socket is at the left top, connect the plug.
i. Installation of Water and Gas Tap（Optional）

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 Picture 10
1. Fastening Nut
2. Stainless Steel Water and Gas Taps
Take out fastening nuts, water and gas taps, installing as Picture 10.

1.5 Checking after installation

First, make sure the Voltage and frequency to be same as logo showing, and then check the follows
items with power on：
Checking Items Normal situation
Inflow 0.53±0.025m/s , downflow
Wind speed display
0.33±0.025m/s

exhaust filter 80-120Pa, downflow filter

Pressure display
80-120Pa

Fan running Normally

LED lamp Lamp lights after pressing button
UV Lamp Lamp lights after pressing button
Display screen buttons All buttons can be used
Press the socket key, multimeter testing
Socket
output supply voltage
Red pedal for glass up, black pedal for glass
Foot Switch
down

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2. User Instructions

2.1 Functions

2.1.1 Product Concept

This products belong to Class II A2 biological safety cabinet which fully meet the requirement of US
standard ANSI/NSF49:2002, European standard EN12469:2000, biological safety cabinet is a kind
of negative pressure filtration system for protecting operator, the laboratory environment and work
materials, the front opening which air flow inward have protection function for operator, the filtered
laminar flow generated by vertical HEPA can protect work materials, what’s more, the polluted air
flow become pure after processed by HEPA(ULPA) filter. When it’s used in microbiology
experiment environment filled with volatile or toxic chemical and radionuclide, suitable exhaust
hood in function have to be linked.

2.1.2 Application Range

Biological Safety Cabinet is necessary equipment in the laboratory in the search of microbiology,
biomedical, DNA recombinant, animal experiment, and biological products, especially in the
occasion that operator need to adopt protective measure, such as medical and health, pharmacy,
medical research. Our equipment provides a safety working environment which don’t have bacterial
and dust in the process of bacterial culture.

2.1.3 Working theory/Air flow pattern and protected area

Picture 11

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2.1.4 Protected objects
Biological safety cabinets (BSCs) are designed to protect the operator, the laboratory environment
and work materials from exposure to infectious aerosols and splashes that may be generated when
manipulating materials containing infectious agents, such as primary cultures, stocks and diagnostic
specimens.

2.1.5 TECHNICAL PARAMETERS

Model
BSC-1100IIA2-X BSC-1300IIA2-X BSC-1500IIA2-X
Parameters

Meet US standard ANSI/NSF49:2002, European standard EN12469:2000

Product Standard
Standard
Power Supply AC 220V±10% 110V±10%

Frequency 50 Hz 60Hz

External
1100×750×2250 mm 1300×750×2250 mm 1500×750×2250 mm
Size(W*D*H)

Working Zone
940×600×660 mm 1150×600×660 mm 1350×600×660 mm
Size(W*D*H)

Consumption 760W 800W 900W

Total Airflow
360m3/h 440m3/h 500m3/h
Volume

UV Lamp
30W 30W 40W
Consumption

LED lamp
12W*2 12W*2 16W*2
Consumption

Downflow
0.33±0.025m/s
Velocity

Inflow Velocity 0.53±0.025m/s

HEAP Filter 99.999％(Diameter: 0.3μm)

Noise ≤67dB(A) / EN12469≤58dB / NSF49≤61dB

Notes: (1) Electric consumption power including power which operation area needs to load (Loading
no more than 500W)
(2) Our company has right for changing the products, if we need to change and re-design, please
forgive us for not notifying you.

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2.1.6 Performance Index
1) Biological safety functions
Personnel protection, microbial colony count ≤5CFU;
Sample protection, microbial colony count ≤5CFU;
Cross contamination protection, microbial colony count ≤2CFU.
2) Leak-proof Cabinet
If cabinet pressurized to 500Pa, the pressure should be no less than 450 Pa after 30 min.
3) Integrity of HEPA Filter
Scan and detect the HEPA filter, the leakage rate at any point should not be ＞0.01%.
4) Vibration amplitude
The net vibration amplitude between frequency 10Hz and 10KHz is no more than 5μm(rms).
5) Illumination
The average illumination is no less than 650 lux, actual illumination is no less that 450 lux.
6) Mechanical performance
Structure design is reasonable, high quality materials are adopted for the cabinet.
It can resist shape global deformation caused by external force.
The working surface will not occur permanent deformation when weight put reaching 23kg.
7) Electrical properties
The voltage increases to 1390V(AC) in 5s and keep for another 5s without breakdown.
Grounding resistance ≤0.1Ω

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2.2 Product structure

2.2.1 Structural composition of BSC-1100IIA2-X

Picture 12

1. Footmaster Caster 8. UV lamp

2. LED Lamp
9. Control panel
3. Nameplate
10. Power supply lock
4. Tube motor
11. Water-proof socket
5. Fuse socket
12. Front window
6. General power fuse socket
13. Inflow grid
7. Power socket

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2.2.2 Structural composition of BSC-1300IIA2-X

Picture 13

1. Footmaster Caster 8. UV lamp

2. LED Lamp 9. Control panel
3. Nameplate 10. Power supply lock
4. Tube motor 11. Water-proof socket
5. Fuse socket 12. Front window
6. General power fuse socket 13. Inflow grid
7. Power socket

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2.2.3 Structural composition of BSC-1500IIA2-X

Picture 14
1. Footmaster Caster 8. UV lamp
2. Nameplate 9. Power supply lock
3. Power socket 10. LED Lamp
4. General power fuse socket 11. Water-proof socket
5. Fuse socket 12. Control panel
6. Blower fuse tube socket 13. Front window
7. Tube motor 14. Inflow grid

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2.2.4 Structure introduction
1) Driving System of Front Window
2) Driving system consists of tube motor, front window, hauling sash and position switch.
3) Air Filtration System
4) Air Filtration System is the most important system of BSC. It consists of blower, supply filter
and exhaust filter. The function of Air Filtration System is transferring filtered air to work
area, ensure the down flow velocity, and keep Class 100 cleanness of work area.
5) UV Light
6) UV lamp is inside work area. So UV lamp can well sterilize all space of work area.
7) LED Light
8) The BSC is equipped with LED lamp. It can make sure average illumination inside work area
which meets standard requirements.
9) Air pipe
10) Air pipe is the ventage of differential pressure sensor.

Picture 15

The air pipe should not be blocked and please do not hang anything on the pipes,

otherwise it will effect wind speed and pressure.

11) Power lock

When the power cord is connected to main power, switch the key for power lock, then the equipment
is powered on.
12) Water proof Socket

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Waterproof Sockets are located on the right side of the work area, which can be controlled by
SOCKET button.

(1) Please make sure the total load of sockets should be ≤ 500W；

(2) The waterproof socket can only be waterproof when its front cover is lowered, and the

socket can not be regarded as a waterproof socket when the front cover is opened.

13) Fuse protector：

The equipment is equipped with main power fuse, waterproof socket fuse and fan fuse (only for
BSC-1500IIA2-X). They are located near the power cord’s outlet. Fuse label is corresponding to the
relevant specifications. Please refer to 3.2.
14) LCD Display（Liquid Crystal Display）

Picture 16
Large LCD display indicates detailed key parameters, it is real-time display to reflect the equipment
working condition, such as effective working state of the filter, which is more intuitive. (Please refer
to 2.3)
15) Control of Front Window
Front window is motorized. It could be controlled by control panel. Glass door control motor for a
dedicated reversible speed motor, the mo tor has a large starting torque, smooth rotation and so on.
16) Structure
a) Biological Safety Cabinet’s both sides and back area are negative pressure air channel. And
the negative pressure keeps work area away from contamination.
b) Cabinet body is built of 1.2mm cold-rolled steel with anti-powder coating. Strong and steady.
c) Work area is fully made of stainless steel which looks beautiful and with corrosion resistance
performance.

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 d) Base stand is made of cold-rolled steel with anti-powder coating.
e) Soft touch type control panel, easy to handle and beautiful appearance.

2.3 Control panel

Picture 17
1.UV Lamp 2. LED Lamp
3.Fan 4.Socket
5.Glass Window Up 5.Glass Window Down
7.Mute 8.Power
9.Alarm Status 10.Mute Status
11.Filter Changing Status 12.Temperature
13.System Working Time 14.Humidity
15.Glass Window Down Status 16.Glass Window Up Status
17.Foot Switch Status 18.UV Status
19.Socket Status 20.LED Lamp Status
21.Fan Status 22.Exhaust Filter Differential Pressure
23.Inflow velocity 24.Supply Filter Differential Pressure
25. Down-flow velocity 26.Filter Working Time
27.UV Lamp Working Time 28.Reservation Timing

a) LCD Screen

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The working status of the equipment and operation can be seen on the LCD screen.

b) Soft touch button.

BSC’s main functions could be executed by touch-buttons. User can operate the BSC either by
pressing the buttons on control panel or using the remote control. There are totally 8 common button
on control panel.

: The power button

: To control LED lamp

: To control UV lamp.(It works only after front window fully closed.)

: To control blower working status. (It will not work when front window is fully
closed.)

: To control socket power status.

: Press MUTE button to stop voice prompt

: Press UP button, glass window will raise.

: Press Down button, glass window will fall down.

Clock Adjustment:
Turn the power key, so machine is in standby state.
Press the light button, and then press the power button for 5 seconds. Then you see the state of clock
adjustment after a buzzer alarm.
Firstly, minute position is flashing, press UP and DOWN to adjust to present time. Then press the
MUTE button switching to hour position and adjust to present time. After that, press the light button
first, and press the power button for about 5 seconds. Data will be saved after a buzzer alarm.

2.4 Remote Control &Foot switch

2.4.1 Remote control

It is inconvenient for the users to operate from a distance. Small & light remote control is flexibly to
be used to control all the functions of the cabinet in a distance ≤6m, 30° range. The operator can

23
even carry it with themselves during experiment for convenience.
This remote control adopt specific chip which is featured with good anti-jamming performance,
longer control distance and high control precision.

Buttons of Remote Control:

1. Power (POWER)
2. Reservation Time (SUB)
3. Timer (INSTALL TIMER)
4. Confirm (CONFIRM)
5. Cancel (CANCEL)
6. Turn up (+)
7. Turn down (-)
8. Fan (FAN)
9. UV (UV)
10. Illumination (LIGHT)
11. Socket (SOCKET)
12. Mute (MUTE)
13. Front window up (UP)
14. Front window down (DOWN)

Picture 18
A. Reservation Time (SUB)
a. Connect power, open power lock, and press the reservation timing button (SUB);
b. Adjust the time (minutes) by “+” or “-” button. Press the confirmation button (CONFIRM) to
confirm; and then adjust other minutes and hours position data in the same way;
c. After the time is confirmed, the corresponding display lamp lights by selecting the function
buttons (such as UV);
d. Press the POWER button again, the reservation function starts. Reserved time starts count
down. The corresponding setting function starts when the time counts down to zero.
B. Timer (INSTALL TIMER)
a. Connect power, open power lock, press button (POWER), the corresponding display lamp
lights by selecting the function buttons (such as UV);
b. Press button (INSTALL TIMER), adjust the time (minutes) by “+” or “-” button. Press button
(CONFIRM) to confirm; and then adjust other minutes and hours position data in the same
way;
c. After the time is confirmed, the Timer function starts. When the time counts down to zero, all
the functions will be off, the cabinet will be in standby mode.
C. Application of Reservation Time

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Biological safety cabinet is equipped with special UV lamp. When turning on or turning off the
cabinet, sterilization time of UV lamp should be at least 30 minutes. In order to save the waiting time
of turning on or turning off the cabinet, we develop reservation time function. It realizes function of
automatic turning on or turning off the cabinet after the sterilization finished. Reservation time
setting range is from 0 to 99 hours and 59 minutes. This function helps operators to save time and
improve efficiency.

2.4.2 The use of foot switch

Picture 20
Press the left red switch by foot, front window goes up, press the right black switch, front window
goes down.

2.5 Instructions for Operation

2.5.1 Normal Operation Notice

（1） Make sure input voltage is correct and stable. The rated load of main power socket should be
higher than cabinet consumption. Plug must be well grounded.
（2） In order to avoid air turbulence, the operator should slightly move his arms during
experiment. Hands should stay inside the working area at least 1 minute before operating. In
order to decrease the times of arms moving into and out of the working area, prepare all the
necessary items inside the cabinet before starting experiment;
（3） Moving principles of different samples inside cabinet: When two or more samples need to be
moved, be sure that low-polluting samples move to high-polluting samples. Movement of
items should also follow the principles of slow-moving.
（4） Samples placed in parallel: Samples should be placed in the cabinet parallel to avoid
cross-contamination between samples and blocking back air grille.
（5） In order to avoid samples being sucked into the negative passage or the blower, do not place
soft and slight samples (for example: soft tissue) on the surface during experiment;
（6） The weight of items placed in the cabinet should be no more than 23Kg/25×25cm2；
（7） Avoid vibration: avoid using vibration equipment (eg centrifuges, vortex oscillator, etc.)
inside the cabinet. Vibration would cause lower cleanliness of operating area and affect
operator protection.

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（8） No flame: No flame is allowed inside the cabinet. Using of fire will lead to airflow disorder,
and filter damage. If sterilization is required during the experiment, infrared sterilizer is
highly recommended.
（9） HEPA filter life: With the usage time increasing, dust and bacteria accumulate inside HEPA
filter. Filter Resistance is getting bigger, when it reaches the maximum point, there will be
audible and visual alarm. Please replace new HEPA filter, otherwise it will affect the safety
performance of the equipment. The used filter should be processed as medical waste.
（10）There is a negative passage surrounding the work area, which is sealed strictly in the
factory. The operator is not allowed to remove or loose screws of those parts. If necessary,
please contact service personal.
（11）Front Grille is used for air intake and drain. Do not block it, otherwise it will affect airflow.
Armrest is recommended to solve this problem and reducing the operator's wrist fatigue.
（12）Long-term use of biological safety cabinets will inevitably cause pollution (e.g. HEPA filters,
corner cabinets, etc.). In order to sterilize thoroughly every 500 hours, formalin
(formaldehyde) fumigation sterilizer is recommended. After sterilization, neutralize
formaldehyde gas with ammonium hydrogen carbonate. Make sure no sterilization gas
escapes during the whole process.
（13）The maximum storage period is one year. If the period is more than one year, performance
test should be done .

Serious declaration: we will take no responsibility for risks caused by improper operation and

man-made damages!

2.5.2 Operation Process

a. Connect the same power reply, as required of equipment
b. Open the power lock, LCD display lights up and alarm rings at the same time, then the machine
enters to standby status. Waiting for the operator to input button to use it.
c. Press POWER button, then the following functions are available: LED lamp. UV lamp, Fan, Mute,
Sockets, Front window up and down, Reservation timing

When front window is open or other button is pressed, the UV lamp can not be work.

d. Before doing experiment, please sterilize the cabinet for more than 30 minutes by UV lamp( the
front window must be closed)

(1) For safety of eyes and skin, people should leave room during the UV sterilization.

(2)UV lamp intensity should be tested regularly. If there is no test conditions, it should be
replaced, recommend once for three month.

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e. Please move the front window at 200mm height from the work table, turn on the fan, make sure
the experiment should be started after fan working for at least half an hour.

For operating safety, please put testing materials inside the cabinet in advance, and keep

the front window at 200mm height from the work table during operation.

After finishing the experiment, please move the front window down to the bottom, and make sure to
sterilize the cabinet by UV lamp for 30 minutes before turning off the cabinet.

2.6. Daily maintenance

Because the operating time will directly affect the judgment of maintenance needs, we recommend
the user keep a detailed record of operating time for reference.

When doing maintenance, please pay attention to cut off the power, so as to avoid electric

shock

2.6.1 Preparations before maintenance

Soap, hot water or warm water, a soft cotton cloth, dry cloth or towel, medical alcohol or other
disinfectants, 100 dilution of household bleach, abrasive household cleaners, sterile water

2.6.2 Clean the cabinet surface

1) Clean the operating area surface
Wipe the entire surface with a soft cotton cloth or towel soaked with concentrated liquid soap, then
wipe up the soap with another cotton cloth or towel soaked with clean hot or warm water, and then
wipe the surface with a dry cotton cloth or towel rapidly.
For the contaminated or dirty work surface or sump., use 70% medical alcohol or other disinfectant
to wipe.

Disinfectants used for wiping should not damage 304 stainless steel.

2) Clean the external surface and front window.

Use soft cotton cloth or towel to wipe the surface with non-abrasive household cleanser.

2.6.3 Overall maintenance period

We suggest comprehensive maintenance period is one year or 1000 working hours.

2.6.4 Maintenance methods

1) Daily or weekly maintenance

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a. Disinfect and clean operating area;
b. Clean the external surface and front window around the operating area;
c. Check the various functions of equipment;
d. Record this maintenance result
2) Monthly maintenance
a. Clean the the external surface and front window.
b. Wipe the working table, inner wall surface of operating area (excluding the wind distributing grid
of operating area) and the inner surface of glass door with 70﹪ medical alcohol or household
bleach diluted 1:100 (i.e, 0.05% sodium hypochlorite). Then wipe again with sterile water in
order to eliminate the rest chlorine.
c. Check the various functions of equipment;
d. Record this maintenance result;

3) Annual maintenance
a. Check the two conveyor belts of front window drive unit, and ensure that their tightness is
coincident.
b. Check the UV lamp and LED lamps.
c. Apply for testing the overall performance of cabinet on an annual basis to ensure the
performance safety. User is responsible for testing costs.
d. Record this maintenance result.

2.6.5 Storage conditions

Safety cabinet should be stored in a relative humidity no more than 75%, the temperature is below
40℃, in the warehouse with good ventilation performance, no acid, no alkali and no other corrosive
gases, storage period shall not exceed one year, safety cabinet for more than a year needs to
unpacked and checked. Only the tested and qualified safety cabinet can be sold.

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2.7 Methods and procedures for disinfection

Details in the After-sale service manual

Disinfection is necessary when any contaminated part of the biosafety cabinet needed for routine
maintenance, replacement filters, and performance testing, etc. Before doing certification test and gas
sterilization, all internal working surface and the exposed outer surface should be disinfected with a
suitable disinfectant. what is more, using Class II biosafety level designated medicament to sterilize
the cabinet by gases is required. Class III biosafety level designated medicament is recommended
after the use of the cabinet. The cabinet, under the risk of being polluted by the biological factor,
should be sterilized before sliding position.
The polluted working surface caused by overflowed and spilled reagents also should be sterilized.
Most of the cases that need gas sterilization use depolymerized triformol as the disinfector as noted
below, the period parameters and its validity of each model and size of the cabinet are required to be
listed before change sterilization method. The relevance of the material has connections with the
absorption and degeneration of spare decontaminant, which is the key factor of keeping the cabinet
integrated and the sterilization time. There need those alternative sterilization way in some case, such
as: slow disease virus. The sterilization method is used against the consult between the end user and
certification authority. Pointing out the given area, gas mask, safeguard procedures, corresponding
test, medical monitoring, conveying danger and training, record and reserve etc... and follow these
steps.

Before sterilization, all the hydrogen chloride should be moved away from the cabinet.
There will result in carcinogenic substance-BCME when it meet with formaldehyde under the
ambient air.
a. Figure out the total volume by multiplying the height, width and depth.
b. The required weight of the triformol will be worked out through the total volume multiply
11g/m³, figuring out the weight of ammonium bicarbonate or its alternative by
chemometry.
c. It must be bubble-tight if there are exhaust pipe with the biosafety cabinet, which can be
realized at the end of the pipe or sealing at the control valve if there is valve nearby the
cabinet, it need more triformol to compensate the added volume if the length of exhaust
pipe is more than 3 meters. If the exhaust recirculation of the cabinet connect with the
exhaust system of the construction, interrupting the connection system of the biosafety
cabinet with construction and sealing (by using plastic film and plastic strips)
d. Sealing by plastic film as the waste gas is exhausted into the room from biosafety cabinet.
e. In order to urgently eliminate formaldehyde, sterilize and eliminate formaldehyde after
neutralizing, putting a tube nearby the biosafety cabinet in advance which is required to
connect with chemical smoke hood or other exhaust devices that is suitable for releasing
the harmful gas.

29
f. Put the heater, such as electric-heating frying pan which can be bought from market,
formaldehyde generator or neutralizer on the working table. Set up the temperature at
232℃～246℃, the triformol is spraying over the surface of the heater devices equably.

The autoignition temperature is paraformaldehyde at 300℃.

g. The neutralizer with the heating device is placed on the workbench. Neutralizers
(Ammonium bicarbonate or equivalent) should be isolated from the air in the cabinet
before use. The following two examples illustrate how to achieve air separation.
Eg.1
Ammonium bicarbonate or substitute is uniformly sprayed on the heated surface of the
heating device and covered with aluminum foil to prevent the carbonic acid hydrogen
ammonia or its substitute in disinfection reaction with formaldehyde. The aluminum foil
should be placed so that ammonia can be released during heating or ready to remove
aluminum foil at the beginning of the neutralization phase. Removal of aluminum foil is
not allowed when the formaldehyde leakage out of the safety cabinet of;
Eg.2
The safety cabinet with the gloves for a whole plastic film sealing. Ammonium
bicarbonate or equivalent substitutes are placed in a container in a safety cabinet. During
the neutralization phase, the disinfected personnel into the safety cabinet through the
gloves do not break the sealing system. The ammonium bicarbonate or equivalent
substitutes are removed from the sealed container and uniformly sprinkled on the heating
surface of the heating device, then the heating device is energized, the ammonium
bicarbonate or the substitute is heated to release the ammonia.
h. Putting heating plate, water beaker and hygrothermograph on the work table, do not
connect the wires to the power supply in the cabinet.
i. Sealing the front operating window with thick plastic film and plastic strips. Seal all the
area where possible to leak. Such as wire outlet, the surrounding of front operating
window and joint between plastic film and biosafety cabinet.
j. Measure the temperature and humidity inner the cabinet.
k. The humidity is at 60％～85％ when temperature is above 21℃, heating the water in the
beak up to the expected temperature and humidity.
l. Before depolymerizing the formaldehyde, strictly restrict entry to the area around
biosafety cabinet or room in accordance with related regulation and safety measures.
m. Connect the wire of the heater with socket outside the cabinet.
n. 25% formaldehyde is depolymerized open the safety cabinet fan 10 s ~ l 5 s. after
the para-formaldehyde is depolymerized by 50%, 75% and 100%, repeating the

30
 above steps. If the safety cabinet fan does not work, Use auxiliary fans to promote
air circulation in the cabinet, or prolong the disinfection time over Step P .
o. Disconnect the power supply of the heating plate and heater for the use of triformol
p. Keep the biosafety cabinet isolation at least 6 hours, it is better to leave it for a night.
q. Prepare the neutralizer, power off the heating device and the fan of biosafety cabinet until
ammonium bicarbonate wiped away, the operation of triformol is also the same, after the
decomposition of 20% ammonium bicarbonate, open the fan for 10 s ～ l 5 s, using
assisted fan or electric fan to promote the air circulation inner biosafety cabinet or
prolong the neutralization time for at least 6 h if the fan of the biosafety cabinet does not
work.
r. Keep the biosafety cabinet isolation at least 1 hour before open the sealing membrane;
s. If exhausting the formaldehyde caused by neutralization via tube, tear out the plastic
cover in the exhaust pipe, connect it with tube and seal. The plastic cover in the front of
the biosafety cabinet will be suck in and cut one or two openings make the fresh air be
into the cabinet if the tube work well, then the formaldehyde is exhausted via tube.

2.8 Replacement parts list

BSC-1100IIA2-X replacement parts list

Number Name Specification
AAQ01 Fuse 10A
AAQ02 Fuse 5A
AAQ03 Lamp holder T8 LG13-01A
AAQ05 UV Lamp T6 30W
AAQ06 LED Lamp T5 12W LED
AAQ07 UV lamp ballast 1*TL8-30W
AAQ08 Upper filter (Exhaust filter) 800*420*69mm
AAQ09 Lower filter (Supply filter) 925*470*69mm
AAQ10 Fan FS133Q
AAQ11 LCD control board (strong circuit
Control panel board, weak circuit board, display
screen)
AAQ12 Remote control (with battery)

31
AAQ13 Key selection button LA42YZP-20B
AAQ14 Glass 1034*730*6.38mm
AAQ15 Foot switch Dual pedal (line length 4 meters)

BSC-1300IIA2-X Replacement parts list

No. Designation Standard
AAP01 Fuse 10A
AAP02 Fuse 5A
AAP03 lamp holder T8 LG13-01A
AAP05 UV Lamp T6 30W
AAP06 LED lamp T5 12W LED
AAP07 UV lamp ballast 1*TL8-30W
AAP09 Upper filter (Exhaust filter) 850*420*69mm
AAP10 Downflow filter (Supply filter) 1190*470*69mm
AAP11 Fan FH355A
AAP12 LCD control board (strong circuit
Control panel board, weak circuit board, display
screen)
AAP13 Remote control (with battery)
AAP14 Key selection button LA42Y2P-20B
AAP15 Glass 1237*730*6.38mm
AAP16 Foot switch Dual pedal (line length 4 meters)

BSC-1500IIA2-X Replacement parts list

No Designation Standard
AAJ01 Fuse 10A
AAJ02 Fuse 5A
AAJ03 Lamp holder T8 LG13-01A
AAJ05 UV Lamp T6 40W

32
AAJ06 LED Lamp T5 16W LED
AAJ07 UV lamp ballast 1*TL8-36W
AAJ09 Upper filter (Exhaust filter) 920*420*69mm
AAJ10 Downflow filter (Supply filter) 1380*470*69mm
AAJ11 Fan FH320
AAJ12 LCD control board (strong circuit
Control panel board, weak circuit board, display
screen)
AAJ13 Remote control (with battery)
AAJ14 Key selection button LA42Y2P-20B
AAJ15 Glass 1419*720*6.38mm
AAJ16 Foot switch Dual pedal (line length 4 meters)

33
 1 2 3 4 5 6 7 8
BSC-1100IIA2-X Wiring diagram/BSC-1300IIA2-X Wiring diagram

D T&H SENSOR D

DOOR OPEN DETECTION

BLACK（Φ0.5）

WINDOW H

WINDOW L
BLACK（Φ1）
T SA LL AB V U

BLACK（Φ0.5）

YELLOW（Φ0.5）

BLACK（Φ0.5）

GREEN（Φ0.5）
WHITE（Φ0.5）

RED（Φ0.5）
UV LAMP WHITE（Φ0.5）
BLUE（Φ1）

）5.0Φ（DER
）5.0Φ（DER

）5.0Φ（ETIHW

）5.0Φ（KCALB

）5.0Φ（EULB
Window
H
L

GND

VCC
H T
UV Ctrl

DNG
GREEN（Φ1） up

Foot
LAMP
BLACK（Φ1 ） down
C BLUE（Φ1） C
BLACK（Φ1 ）

NAIJNA
MCU EIGHT CORE CABLE
LCD
TRANS-IN
GREEN（Φ1） L
LAMP 1 2V/0 .5A

34
BLUE（Φ1） N EX-FAN
GREEN（Φ1 ）
L 1 8B2-EF GREEN（Φ1 ）
N TRANS-OUT SPEAK
L

VU
N
SIXTEEN CORE CABLE
L

L TEKCOS THGIL
SOCKET
FUSE3 N
RED（Φ1） RED（Φ1） SPEAKER
L
5A N
BLUE（Φ1）
YELLOW GREEN（Φ1.0） BROWN（Φ1.0）
）1Φ（DER

DOWN
ROTOM ROOD
BLUE（Φ1.0） DOWN-FAN N UP

N
L N
B SOCKET B
YELLOW GREEN（Φ1.0）
BLUE（Φ1） BLACK（Φ1） BROWN（Φ1）
YELLOW（Φ1） BLUE（Φ1）
FUSE2 DOOR MOTOR
BROWN（Φ1.0）
L

WAVE FILTER
10A DOWN FAN
2.9 Wiring diagram

POWER POWER LOCK

YELLOW GREEN（Φ1.0）
YELLOW GREEN（Φ1.0）
BLUE（Φ1.0）
N
A A
1 2 3 4 5 6 7 8
 1 2 3 4 5 6 7 8
T&H SENSOR

DOOR OPEN DETECTION

FOOT SWITCH UP

FOOT SWITCH DOWN

BLACK（Φ0.5）

WINDOW H

WINDOW L
BLACK（Φ1）
T SA LL AB V U

D
BLACK（Φ0.5） D
WHITE（Φ0.5）

YELLOW（Φ0.5）

BLACK（Φ0.5）

GREEN（Φ0.5）
UV LAMP WHITE（Φ0.5）

RED（Φ0.5）
BLUE（Φ1）

）5.0Φ（DER
）5.0Φ（DER

）5.0Φ（ETIHW

）5.0Φ（KCALB

）5.0Φ（EULB
1 3
2

Window
H
L

GND

VCC
H T
UV Ctrl
GREEN（Φ1）
LAMP

DNG
BLUE（Φ0.5）
BLUE（Φ1）
RED（Φ0.5）
up

Foot
BSC-1500IIA2-X Wiring diagram

BLACK（Φ1 ）
down
BLACK（Φ0.5）

NAIJNA
BLACK（Φ1 ）
MCU EIGHT CORE CABLE
LCD
GREEN（Φ1）
LAMP
C BLUE（Φ1） TRANS-IN C
L
EX-FAN 1 2V/0 .5A
N
GREEN（Φ1 ）
L GREEN（Φ1 ）
1 8B2-EF
N TRANS-OUT SPEAK
L

VU
N

35
SIXTEEN CORE CABLE
L

L TEKCOS THGIL
SOCKET
N
FUSE3 RED（Φ1）
RED（Φ1） SPEAKER
L
5A N
BLUE（Φ1）
YELLOW GREEN（Φ1.0） BROWN（Φ1.0）
）1Φ（DER

DOWN
ROTOM ROOD
BLUE（Φ1.0） DOWN-FAN N UP

N
L N
SOCKET

YELLOW（Φ1）
YELLOW GREEN（Φ1.0）
B B
FUSE4
10A BLUE（Φ1） BLACK（Φ1） BROWN（Φ1）

YELLOW（Φ1）

BLUE（Φ1）
FUSE2 DOOR MOTOR
BROWN（Φ1.0） BROWN（Φ1.0）
L

WAVE FILTER
10A DOWN FAN
POWER POWER LOCK
YELLOW GREEN（Φ1.0）
BLUE（Φ1.0） BLUE（Φ1.0）
N
A A
1 2 3 4 5 6 7 8
3. Trouble shooting and Labels

3.1 Common faults & solution

3.1.1 Warning and reminder

Digital display of pressure difference, digital velocity display, audible and visual alarm system.
1) Over safety height alarm for front window
There will be audio and visual alarm when front window is lifting over safety height. Same time
LCD display will twinkle exclamation mark. Then just adjust the height of the front window.(Front
window height setting value is 200mm).
2) HEPA filter pressure difference alarm
There will be audio and visual alarm if pressure of air supply filter or exhaust filter can’t meet
present value, at the same time LCD display will twinkle exclamation mark. Remind the operator to
replace the filter immediately to protect the operator’s safety.
3) Velocity fluctuation alarm
There will be audio and visual alarm if the inflow velocity and down flow velocity below 20% of the
standard value, namely, inflow velocity below 0.42m/s, down flow velocity below 0.26m/s, at the
same time LCD display will twinkle exclamation mark to remind the operator pay attention.

3.1.2 Trouble shooting

Please confirm whether the power is connected or not, whether the power cord is obvious damaged
or not, whether the fuse is good or not, and whether the power locks are in the open state or not
before the fault diagnosis.
Faults Check parts Measures

Circuit Check circuit

LED lamp doesn’t
work LED tube Change it
Control panel Change it
Front window, LED lamp and Check the front window, LED lamp and the blower is
blower open or not.

Lamp holder Tube and lamp holder is connected securely.

UV lamp doesn’t work
Circuit Check circuit

UV lamp Change it

36
 Micro Switch Check if Micro Switch is broken

Control panel Change it

Make sure the power connects well and the fuse is

well
Check if the button is broken
Button doesn’t work Control panel
Make sure the connecting wire is connected well

Change control panel

Front window Front window is open or not, blower works only

when the front window is open
Micro Switch Check if Micro Switch is broken or works fine
Blower doesn’t work Blower If blower is broken, change it

Circuit Check circuit

Control panel Change it

Socket fuse Check if socket fuse is broken

Socket Check if socket is broken

No electricity in socket
Circuit Check circuit

Control panel Change it

Pressure or air speed Gas circuit Check whether gas circuit has dropped, is broken, or
displayed incorrectly jammed
Control panel Change it

Circuit Check circuit

Motor of front window Check front window motor

Front window doesn’t
work
Transmission part Check transmission connection and lead rail

Control panel change it

Foot switch doesn’t Circuit Check circuit

work
Control panel Change it

Remote control Check if the Remote control is broken or not, and if

Remote control doesn’t there’s electric in the battery
Connection cable Check whether main control panel and display board
work
is connected well.
Control panel Change it

Power supply Check power supply connects well

No electricity in
Power wire Check whether power wire has obvious damage
equipment
Fuse Check if the fuse is good

37
 Power key Check if power key is open, is broken or not

Transformer Check whether the transformer works normally

Control panel Change it

Connection winding Connection winding displacement

displacement
Display doesn’t work Display screen Display screen

Control panel Control panel

Micro switch Check whether the micro switch is good, and it works
normally or not.
No alarm Circuit Check whether connection circuit of micro switch is
good.
Control panel Change it

NOTES

(1) The above electrical parts must be operated by a qualified electrician in safety conditions
(cutting off power supply). The other parts are not allowed to remove; otherwise the user
should take responsibility by them;
(2) When failures are not occur, and the operator can’t solve, please notify our maintenance
department immediately. For your safety, please do not maintain equipment by yourself;
(3) The maintenance of this equipment is undertaken by trained and recognized technicians;
(4) If you need to order parts, contact the agent or our technical service department, and please
indicate the model and serial number of the cabinet purchased.

3.1.3 Simple accessories replacement

1) Replace the fuse
Socket, fan fuse (only BSC-1500IIA2-X has fan fuse) are located in the top operation panel. When
replace them, turn off the power and disconnect plug, use a Phillips screwdriver counterclockwise
pressing screwed fuse holder, remove the fuse out and replace a new fuse, and then clockwise
pressing screwed fuse holder; FireWire fuse is located in the side of the cabinet operation panel, take
out of the fuse holder using a slotted screwdriver and replace with a new fuse, and then press it back.

38
 Picture 20

2) Replace LED light

When replacing lights, make sure that the power is off, open the operation panel like shown in
Picture 21, use the control panel support frame (fixed in the inside position of the control panel as
shown), then like Picture 22 shown screwing the tube 90°, take the correspondence type of lamp, put
it to the lamp holder and screw 90 °to the right position.

Picture 21

39
 Picture 22

3) Replace the UV lamp

UV lamp should be replaced regularly according to the frequency of use, when using UV lamps
reach to the time of 600 hours, we recommend to replace the lamp. When replacing, first make sure
the power is off, and then screw the bulb 90 ° and take it off , then take the correspondence type of
lamp, and put it to the lamp holder and and screw 90 ° in reverse direction. After replacing the UV
lamp, it needs to keep pressing the button of UV for about five seconds when the machine stays
standby.

Picture 23

40
3.2 Label Description

1)Biological hazard label (Picture 24)

Picture 24
2)Fuse label (Picture 25)

Picture 25

Note:

a.10A power fuse label

b. Operating area 5A socket fuse labels

c.10A blower fuse label (for BSC-1500IIA2-X)

3）Ground label

Picture 26

4）Glass door super elevation warning label

Picture 27

41
5）UV lamp alarm label

Picture 28

6）Load requirements label

Picture 29

7）Exhaust filter upstream label

Picture 30

8) Downflow filter upstream label

Picture 31

42
4. Warranty
1) Warranty is 1 years from EX-factory date (excluding consumable accessories, UV and LED
lamp, fuse).
2) We will take no responsibility for risks caused by improper operation and man-made damages.
3) After the expiration of warranty, our company is also responsible for repairs, but the
corresponding maintenance cost should be charged.
4) Life time of biosafety cabinet is 8 years from production date on the label.
5) We can provide equipment drawings and necessary technical data for maintenance companies or
personnel trained by our company.
Warranty declaration:
One-year Warranty, Life-long Maintenance.

BIOBASE GROUP
Add:No.51 South Gongye Road,Jinan,China
Tel:0086-531-81219801
Fax:0086-531-81219804
E-mail:[email protected]
Web:www.biobase.cc

43