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PHARM4oLOGY-
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A QualityProduc
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9nDE Standard
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Sr.
No. Experiment Page Date of Date of Remarks
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Experiment Submission
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1. To Atudy the commouly wedutru-
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meu iu experimeulalphamacalogy

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2. Tostudy the differeit roulesof drug.

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admiuirhrliom nfor auimalAtudien
mice
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3.Praloal deign wuith
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o
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speial refereuce CPCsEA.
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.To Atudy the Conmnion laboralary
auimals
S. To Atudy he auaesthoic aguf ued_
Ju auinmal Aludy.
Atudy the effet of various drug
6.To
on rabbit eye uing Ex. pharma
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SDftuare
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7To Atudy the effed of drus nJoo-

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A

molkr dotiuily_uxinq Ex. pharm Soft

H

ware.
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8To Atudy the vauia, pracedurea for

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rudening_auinmal wcomiauA Atuuin

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9 o rodeah.
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9 To study the dffeaive separion af
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Aerum and plakma,

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C

10 Td Audy the blod sampla Calle-

Hon AAmall Jaboralory QunMalk.
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xperimel to: OD1
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Aini: To siudy he co wocd Jusnuweuls Juu eperimentas paronia
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(alogy

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Reqiromu Orqan balh Rota rod, Amalqesionielu f photdmeter
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iecconvuiio moer, Coot's pole dimbing apportu,
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Roto Rod
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Actophotormclor-
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Date
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Expt. No.
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Page No..
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txperimetuo:- O1
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Aim: To
Aldyue_tonuuouly ueclJuiliumeu J experi
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meldalpuarnaCalog.

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Rafereuce :> udtazui S.k Houdlbok oexperinueuda puaanuacalogy
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Vallabla prmtaluau
4 editiou 6qe w
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KequureMOL:
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Orqau ball RalaAod.Aual geionuele-Aeu
pMntbiuel EecRs couNulaió mote CaDE'S pole
Clinbiug appaialiu
heory:+ Koa Rod : Oug de impOLau pharuatologycal
acus Í dueaud. auxity aqela
o beLgodiazepúue clau of duq
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usclee releiu properly
ue
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Acelstal muscle relastati toguluor uih taniug gfet

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H

Ato patomeor:>The Jackometrk aaivtty Cau eauuly Meu

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Lurec UIg_0re adepliatomeler ulucu

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or cells ulicu aua Cuuectedl_

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Operae3 pliatoelectic.
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cisit wta_coudtulutu e beomoLiatt
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fallug c theplaatocet s W
of byike aunmal
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COatsecorded.-fAuaclopkatormele could Lave

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eelker Lcula uluc ulucu_tluo aunmal MOves

C

Orqau bau The ue bath uand o_2u e auimal

tiue for zludyiustuadio _colled
Zludutorgau bat was diuiqued by Rudalp
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Orqon l3äl:
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A- ube

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blh
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Owex Oxyge
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Thermaxtale
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Date
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Expt. No.. Pagc No.
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Plaquu w 1904 ue crgauball especiallyCouuickaT
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a). Au cuter acte_

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nmade upoff_ Atiel gla

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b. uter CYqm mada tup affglas utu
he
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om 10 m JSD mi
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d Stie do ceepue uaor utor-acreat ar uuifum
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temperiue
e)Oxygeu tiube ulidkaluo sorves atuue uolder
f Cua_coul,oe eud of ulucl iu Cowneond o ud to 4he|
Cõufaue uawiugplayaialogicalAalt saliulie.

Eleztro couvulaimelor u us apparaa eletinaloch Ju

ue
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appliediunugh curneal Teckoclos.
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ThAougloptirAuimuletimcorical excitalion produced.._

DiES)_LmaximMal elecuo-Auock Cauvulioul are devided.
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Tue
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UMo five phausuch

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aa. auic-filialion
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b Tnuic etoui
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Clouic
ClouicCOuvuliov
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d upe W
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e. or doatl
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Recoverivg2
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C

Cobks pale climbiug apparalis 1k la employed torthe

Zludyof Outiseptic cuug.

t a ued to udy coquative-functio_Mauty a

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Cook Palo
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vi
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Cimbiuy Appardut
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i
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Cedritug
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Aalgeriomele
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Rexul laboratory appliaert

ed u he pharmotaJo9y uwore
Studied 9 ulere
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Date.
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Expt. No.. Page No..
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ebU
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rApaueJo Couditio Junul dluu? Joaviy_audib
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cm
apparas has au eipeimouda
W cluamlper25X2SX2S

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LT he lorgid a sod ptooi ntlosure.-

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Coatuifuge: Rapidly ralalug) Coxaiior that _opplie_couatugalL
ofur_perpeudi-
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Aepaude
fone to ita couleutdrpically
-fluid anus. applyiug2 a
spaiu
paudiallyIhhin
CulaTb aleaof
Aualqesiomelir:
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Rapid aud prèikesueg aí aualheic. cluriug2 poperties
M

Small Jaboratorie auuimali.

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A

Orid iu Lat plale eAualgesionTalir has beeudeveloped

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or lasiug ihue plaw, -

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ua o a MeniuremPL oiT aumals rauitauce.

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trourparel
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Result sue dahoralory applinueo ued u due plharnia
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Audied
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Coloqy_ulare
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txpenieul uo.02
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-fim:To Atudy e diffedh rouWe df drug odnuuwh alin Jn uce,
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nuce
s Hopdiuo
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Auua pantoues
adniuixtrin
of dny
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Date
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Expt. No. Page No.-
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=
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txpenret vo:-02
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Aim o Aiudy
e differCut rauo o dug adniniulaaliu d
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nice

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Keferuce!> kulkau St Nallali Prakauau uaudbt experinuaal
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puamacaloqy ane uo
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Kequiremeul:Auinal Dlice geliM Cauda, elc

Priuiple:> Tue e_aud duaioa adioL of_ duq depeud aw

oule off adnuuikfias, e.q. ullkeua druq giveu
byulAveuou_ule le effec s AuL atareucuuly cr
Compcuud to_cral adnuuutaticu off druq uleu ake.
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ipto 1 uuL Awuw
le effec.
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Route: 1).Tuoveum
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2). xal
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3. Tuapesidaueal
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Proedure:
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aumadsQud uun»bered uem o

W
1)._ueiglut e ueJail.
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2).Dividee acn> uto dlueegrepe eacu ofCouuULing 5 nice

C

for uile peidtaueol.iutiaveuous aud ora oulesaf

o
aduuitati
e
3Tukaperidouealiujeilim umade obdome uulkeue
*

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(
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Ora adiuuralian oí druqa
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uluaneuou adnuuuhaim W
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ot cru
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RexultThe differendOul ofdruy .a.dnuuutrabon M

nicewone udied.
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Date
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Expt. No. Page No.
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a tue doraalJau
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N _used for dicNCLGL adnuwa
ora mouuo by
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tia for roule uald Le Jkucc nnuclk auud
ucedte o
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plate uefecdiug Le douque audqgenlPUs

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thusAaltica u ha moulh

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).NatRie Jlu ime c dujectio aud time of aet afJeep.
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u
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tuuieJlue aumal Aleeps._pu uutgu backAo uat ukein
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t_regau ccuicuueuit uult oyer Jo uunnad
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poime.

5 Nate tuo tinne ol recoveN ud calculate dho_duialian CT

actio

6). Compare dlue oT A actia aud dtuatia ot_AlLep_duc te

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piusborbital radùum uhengivo by dffercut rOule af adniuw
M

aticn
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H

Result:Tue ditferet rOulo of drug aduTtiatiau nuce

P

ulere iudied._
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Ex peimAdnDO?
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Fefereue
Pratsca deigu forauinat Tuclies illpecia1
fim
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Date
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Expt. No.
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Page No.
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Experimout uo
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03
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Aim:>_Palora deiqu for auinialtudiu llApecial rqerenee
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CPCSA

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RefèrevuLe i> cultaui S.. uaudbaok dd expeumeulal _pharnia(logy
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publied by allabuprokahau uh edticu 2012)
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TheoryCPCSEA: Comnittee fer
Auperviiouo
ue
purpoic ar
CGUdral auel
experimovitk ou aunmals
Tu Lomnuittee ha differud aplicaticu i auimal proure-
nmeul

Aumal procuremout:2-AU aumal nut be aquired Jawtulle

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a
M

porhe CPCSEAguideliuO
Atcallh
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suvellaureprmgram forAcreeig uconiug)2

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auurmalsuould b0 cauied Cutto aieK auma auali.

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P

QuramtiuO Stabilizaliau aud Separatiou> Ourauliue i

L

ueuty
L

Beparaünoi_
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recieved auinad frm uM aliedy u_uefacilitup Ltil_e
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Lualtu aud passiubly microbial Aldu of he uCul recievee W

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aumals have beeu olatemuuecl.
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C

Auuma care audTecdauica Peraonal Nunat Ccal p2auanwu

requireochuical auel
uubaudAy Suppo ALDaLal migld omploy peopla Ier
baLle fomal aud djob aiiug? uue
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Date
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Expt. No.. Page No.
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effeative umplomdalion oÍ ue_peg.an
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fuma Experimeudtatiauuvalviug Hazarcdau ayei :4 1uidiui

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Auould uav
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paioe cenuua experimeatati h hazardou agukTatilu
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iaal Committee Llleso member are bucuuledgahleabout hazoj
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Jduagulk.
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Duriug of epenmezt No oumalAluoudd be ued for expeinieu
lalicafer more fuan 3 ycar ltss
adequale CTficati pmvided.

Puyaical restrait: pluyaical_resltai er

auinal for exa-
a
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miuaia Callectia of samples aud
M

Vaity of cluer cliuidlaa,

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Fudlianal_areaM:> She aud Latur ot atacility ui difer-

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uue wnatlerarea to Aeparale Aenice

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functiau arcpostuble Cr uecceLor

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L
L

Puaical Faciiitie:>
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Kuldiug hould _beAoledecdto facilitale cfiieut
mettnal
R

yqieuce_Operaio4f auimaly-factitie,durable.
A

aul
C

maictuc put fire reÍKlant,

oImdorc slLculd bC uidc uOugldofacilitale s

mavemu
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Expt. No0.
Page No.
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iiiesAudh as ualer iues draiu pipe aud electneal cauno di
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ould retarabl be acceuble lucugAevice paues
paels
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iMauls room_dorsshaudbe utvemiue aud du prml

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Exterior wuudow are uat recommeuded fur mal oumal fad -|
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itig.
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m
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flourMuld be n
moliureprTot audAlLA_pratL

al Auould be freeof Cractes wealed,_

EuviDuMeut:
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Tempratire ad Humidty Codrad:Tdeay Capabitity Aueld be
M

povidedto illow vaiaia

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lu uthemuge otapprmx 18°C 29C

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H

Veuctilatiou: Tu or u buildiuguew
reuOValiug exictiug
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the Vedilatian of she auinmQb_primary utlosurs.

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Noue Contini : he facilidy Siould be providod itle uaue

W
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E
ree uvioumo uoue (OTulkaduimpataut
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R
A

CCLLSideratta udosiquuug/ du auumaly facility,

C

Aumal Hubaudary :>

Cogiugor housiug Aem 4me caqug aIom ea

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mbztuporlead elcneu
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|S0cial euvironmd":> ueoial eUAOumeud ucludos all dc

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uuteradkiau amouq udividual c a qaup
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u-
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Fwd :>fumasAuould be fed palatable (outamivaled
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aud uluilieualty Odcguot
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Waer: Ordiuasly umals Auould uave Cdiuos acceto
fres patable Acaufomuualed_diitiug_tuater accar
duua ueurparlicular requremau,

Stoudardoperatiug_procedures Guideliuek2eutuke Aual!

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maiilau SoPz des
M

crilbivq procedures motuecla adopted ll eaarcdto animal

R

wsbaud
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H

aud-Luthauaia ia
P

Auaethesia
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L

Anaertheia Uu coüliauy
da he aclúavcmruul_T ile
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Yesulty DZUd eclalive ualguic aud
W

Quodheia
W
E

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Eharaia :> t Ju euorted tlo veuli tare Q auumal

C

requiredto be sactiÄtd outom-=

zation of experimou

Bree diug aud TMgic For uiliatu a Calouy Hic

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breediug Stockmul be_ procurcd rom CPCSEA reaulered
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Aim: xIludyLie Comia_labeadai auumanls,
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Taricity Sludies ReTinatoualy
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Local-Auacsilpic
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louse ( My- musules )_

Characdernatics
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Datc
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Nory Amall Uu AIne.
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Ccounuic ud eaiy lo uaudle aud maiilaiu
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Deied romJue _uild mouse mus mucudus

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Study of cdrugs alug C CNS)_

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Characteritiu:
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tut Aample.
conue iu abiouut
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Respisclony Fati HoTo 11mivte.
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Date
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Page No.-
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idal Nolunie 0.Gnl
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L

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Piocelfor diab mliu3
Sudytue efect of uypotuennua
Poas tarch elaled to lueimmuralogy caucer aue joLvalogY Suclies

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erntz.4AEARn
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quuc viraloqy
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| Bio auay of prosaqlaudw
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life spa 3to S Years.
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-
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Sudy ai vilanún
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or orycalaqu
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Docule auma-Adult uciglki 1:S3.0 f
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S19 as Clha earvesso
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uuere Aludiec
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e puammacolog.
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Expenméed uo, ops
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Aimi2oAudy e auaestuetic ageul ued iu auintal ude
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eqDix tlnlettuor kelbniue
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A

A
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onumauly used (aberatoryAuastuesia

EHAyle (orboniuo
Peutabarbita
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3) Phenobarbital Sodiw
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uParaldebyde
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s)Icolomiue uydrocllaride.

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dilalias Qud pulmouauy CoLYecliou. Lcoulractiou

Disadyaztage :DogTt iat.Luueu

61
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salutdM 1.2 nd ip
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Etans
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Cal 6. Satülic 2:1 mlm
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Resull i Te auacatu.aticageul wed u_auimal dudy
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xpeunw uo- 06
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eye
Jhe eflet of various druq Ou rabbu
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Him: To study

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Kquiremont Rabbit. eye droppe, rabbil uoJder drug Aaluiioa
0-3 7 Adenalu. 27. Puysatignuuue. 17. fAtropie
amdl 17. phedriue
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Expenmui uo: 06
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Aimi To study ue efled variou druq au rabbil eYe _uIug
L

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ol

L
EXplham softtuware.

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Rcereuce i> ulkuui C.E. Llaudbaut attpennMOudal _pluarniacola-
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H editi Vall ablu_prakaskau.
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C
Requremeu: eye droppera
Kabbit rabbi Lolde drugAaliücu
0-1.Adreualiue 21. Puysastigniue 1/, AHro -
piue auel 11Epluedriue J.

Theory:+

ae ued far tueir

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Pruuple: A large Lumber i drugs
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docal cian d e Rye a ye dopsroupsioxx

o
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o Ahlomu_ud craiu age o
aqecus
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Ltetaeu s cleeteated preAMure.

Coustriaioof pupil by oppastlaActios
h uill ucreasing
draage aud veduce ufralar presue.

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(oawd ofor

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Saline Nornial Preol
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Eptaediue Dilatia Present Pre et

Atrpluue Diuaion Abrut Abred

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iu
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Proedure:
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1).Platetuerabbite bou ceepiua he heac uAide,

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Observele size o_pupi
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lulil fu dropsof autkopliue, Aal, u
Coujuctian over pernod a
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Hhe rabbi aud leH eye's
rabbit dould AeNe a Coutrol
Kocordthepiupillan siza _ligut Fel,lex _aud corueal refleu after
Lnuu o drug wtuUatioa audtabulale obaervato
Repeat o expeimout mk playAasligniue_aud epte driue
aud fuer clrug
A

A
M

Result:The effect of yaliou druq o tabbit eye uiug2

R

-Ex pham coft uare uas zludiedAucceufully.

A

A
H

H
P

P
L

L
L
E

E
W

W
E

E
R

R
A

A
C

Teccher's Signature:.
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 Page 36 of 47
CAREWELL PHARMA - A FAMILY OF LEARNING
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A

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M

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R

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txperimeul no.07
A

A
H

H
P

P
Aim: To udy Jhe effecd of druge oAJoco rm
Dir aivpb
L

L
UAin Ex- pMasm Aofiua.

L
E

E
W

W
E

E
R

R
Obzervauoue
A

A
C

C
CLucuege i ativit = Pror drug(doconolor aliinPot dnugx1
Pr rug

S.uo | Daxe LocomOt1M

Paxl druug
Chauge
activiy
u
fre-dru
A

A
Veluicle 13031 26
M

2 Smg ta 13S 64. .8?.68

R

3mg F lo.02
A

3mg 6
H

12.+83
P

S 3ma
L

L
L
E

E
W

W
E

E
R

R
A

A
C

C
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R

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 Page 37 of 47
CAREWELL PHARMA - A FAMILY OF LEARNING
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A

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Datc
M

M
Expt. No..
Page No.
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R
A

A
Experimut un:a 0t
H

H
P

P
Aim: o tudxue effectof drug ou Jocomeler aduyu
L

L
L
iup Ex- pharm Soltare

E
W

W
KeferuUce cullcaru SK, Haudbwt of xperinieudal pharniacology
E

E
edittic 2012Nalab Prakasiau.
R

R
A

A
C

C
Regurmeuts Aumal : plice
Apparatu Aatepuorme, drug

Theory :
Priudple DTl of (NS dia aflect Jo cametor gdivibe D
A

A
M

1). timuaubAA (dffive ample, amine tc.

:
Rarbitutalos alcuoa t.
R

2)Deprameuds
A

Lecometer activity = ude of wace fulueu.

H

H
P

CoLurtructiou:
L

L
L

u
E

E
Sin bui- pkalo diode
W

four digit digilaldispla W

E

E
Couduicliw1g rocu -for ilucorpoFatiug elecric sluock of upto
R

ra.
A

100 Va for adivtiuy_

C

Jalorciug Gpre opërales oM pMalo electric cel0 Couuetted

Lu Uruul wwtu aCouuler

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 Page 38 of 47
CAREWELL PHARMA - A FAMILY OF LEARNING
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A

A
Date.
M

M
Expt. N0.- Page No0.
R

R
A

A
H

H
Procedure:
P

P
L

L
L
1).Luleigbtle aLunialu aud uumber tlem

E
W

W
2 uM ouulue Rquipmau aud make Aure all PTclo cells ualdui
3Place udvidual cage
E

E
mouieLu_activüty tor dipuläled ime
R

R
Qud aud uato e baal activity Sme for al auinnay.
A

A
). 1uededtue drugchdorpusonaziue L doxe: 3 mg l1k.p
C

C
S).Calculate /. docreae u malor activity.

Result:>Tue plecdofdrug ou Joconiator aclny iug Ex-

puam Saftuarg uaAtuclied AuCceriully.
A

A
M

M
R

R
A

A
H

H
P

P
L

L
L
E

E
W

W
E

E
R

R
A

A
C

C
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R

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 Page 39 of 47
CAREWELL PHARMA - A FAMILY OF LEARNING
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A

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M

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R

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txpeimnt mo- 02
A

A
H

H
}he vanOuA proceciures
or reuderuiug auimal
P

P
Aim i To ludy et
otrod
L

L
wCOucIous Atuiug

L
E

E
W

W
E

E
R

R
A

A
C

C
A

A
M

M
R

R
A

A
H

H
P

P
L

L
L
E

E
W

W
E

E
R

R
A

A
C

C
A

A
M

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R

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 Page 40 of 47
CAREWELL PHARMA - A FAMILY OF LEARNING
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A

A
Date
M

M
Expt. No.
Page No.
R

R
A

A
ExpeiMeul uoa 08
H

H
P

P
Aim: To sudy ue VOiOyprocedures forFeudenug: aunual
L

L
L
UMCOuAiou suiuy of rodeul.

E
W

W
Refereuce :> kud karui S. Houdbmk of -€xpeimézdal Pharniacaloc
E

E
eciticu 2012
R

R
Vallablu Prakasua.
A

A
C

C
Theory 2 Uiug?_Chenuica aud plyaical eudhauauia

Ethauauia :s Itu tho at ot ududiug unau doatu iu a

QuinmalAacrificiug -the expenimeulal ouimal after
after
Le by geulasprocedure Coauuiug miinial of pysica aud
Stenug Callec euulu auauia
A

A
M

Muthods:uere are kof tuo_motluoda..

R

R
A

1).Cenical: Tuhalou reaqed- rLer ualonatertumue chloroforn

H

H
P

P
L

Tujectable_ageud g Celantiue Chorouydiatel

L
E

E
W

2) Puyaical i_ W
E

E
R

R
A

Cervica DiloTaio-
C

Decapitatiou
ui
PiHLug

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 Page 41 of 47
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A

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Date.
M

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Expt. No. Page No.
R

R
A

A
H

H
Cerviual Duloaiau :
P

P
L

L
Advailage : It

L
doesLiat chenically coutanuivate liue uis

E
Tecuuque luduce rapid Jau of couuuauueu4.
W

W
E

E
Decapfatiau: T cau be uued for rodculk aud Anall rabbit
R

R
A

A
researchotf iuqs.
C

C
Advazdage : T doe al cuenically coula ceuau coutaniviate
uueHis techuique udute rapid Jau lka of
CouCiOuuuos.

Duadvaudage: Tustecluuque iy bo dutreutul to_cuunals.

A

9 Auual ma be uuod a
M

Stuiug by blow lo the uead

By te ue of
R

u-_peLelraliug captive hald

we O
A

by tha electicCurret
H

H
P

Pituiuq JJu xed for procedure Jo euuure dleatl af

L

umal uad Las beeu

L

reuderec_ cOuuuQus
E

E
by aluer meau
W

W
E

E
Rault The vaiou_procedue for reuderiug auinual u
R

R
A

Coucio ATuUug olrodoulj xludied.

C

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 Page 42 of 47
CAREWELL PHARMA - A FAMILY OF LEARNING
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A

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M

M
R

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A

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H

H
sexum Ond plama
etfeive Aeparatinn of
P

P
Am To study 4he
L

L
L
E

E
W

W
E

E
R

R
A

A
(Oulaiuiuo
cOulaiuiug
KPgvintmort:- Hume blod Aample, Naruouer ube
C

C
:appropriad
i cauguwa, Aeradogical pipoleu of AuAiug aud
Valume,Crywal bncldop cntfuge
,

rolvr
A

A
M

M
R

R
A

A
H

H
P

P
L

L
L
E

E
W

W
E

E
R

,
A

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C

:
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 Page 43 of 47
CAREWELL PHARMA - A FAMILY OF LEARNING
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A

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Datc
M

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Expt. No..
Page No.
R

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A

A
ExpennM d uo:a 09
H

H
P

P
Tm:3o ud uus atfedlive eparalic o srum aud
L

L
plamia.

L
E

E
RefereuCL : Prulocalu for_prepaialiau
W of blbud
plainua Cud Serum

W
WWU. au -Org. com procedue lorAeparaluaia_plauma
u
E

E
aucd_Aerum
R

R
A

A
C

C
Requremany _Humau blodamplovauutaiuer tubes CotUUIg_
auulicoaqulau. seralogical pipetteal appropviao
Valume Croviat beucdalop ceutnfuge ulh_suIug Aud Yalor.

emy Blmd planua ueiquide iquide_compaueu of hlnd i

mluichtho blbcla ceils are supudod 1 mates
abou
A

A
o1 of dotal blmd_yalunie C901_udCouiai
M

dimolved p/aleius glucae_aud nuueral ious

R

R
A

Seum dL cleatueu planua of fetwer protiesprotieu ave

H

ACme Cousiderdasuderjaciug subtauces AoMe_

u
P

HelsaA uey vead

utl veaqed audMere by ield uu
L

L
L

accuFO rOSUlts.
E

E
W

Procedlure: W
E

E
R

R
A

Separatioa of pluma
C

1). Callectue blodJd puple top EDTA tube aud cordni-

uge 206D RPM2 tor 20 nuib.

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 Page 44 of 47
CAREWELL PHARMA - A FAMILY OF LEARNING
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Date.
A

A
Expt. No..
M

M
Page N0.
R

R
A

A
H

H
2).NHrCeidbilugatio UKiug
tleau npelletecuui.qu0lace
P

P
plainia iuto :S mi-Eppeudro ubeJabelled uillu.
L

L
LLactiugLLumber

L
Ludplaima.

E
W

W
3).Tecze ummediall d eoC u freezer.
E

E
R

R
B.Separati on_df Serum:
A

A
C

C
1).Called 1ond ulkale bladcuiugseum Aepaater ube fscTI.

2.-ALlouw Aample to clot for1 uour_a romtemperlure.

3CelHfuge for 10 nuud appxoxiniadcy 1000 RPDi.

A

A
M

u)Usiug cloau_pipette Jecuiquoaliguat 210 UL ot. serwmÚuto

R

Aobelled Chyovial.
A

A
H

5Tmmidcall -freezeviak af serum d Go'Creezer

P

P
L

Result: uesepataliou ot_Arum_aiudplaina ludied

L
E

E
Auccessullkp.
W

W
E

E
R

R
A

A
C

Teacher's Signcture
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 Page 45 of 47
CAREWELL PHARMA - A FAMILY OF LEARNING
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Expenmeul uo:- 116
H

H
P

P
tie btoocd Aomple Collecliu JA Mad
Aim: Totudy
L

L
Jaboraor auumali

L
E

E
W

W
E

E
Observaioy :
R

R
A

A
.
C

C
Ainial S
uart :Dtedium Lou
SDeciea Auutheia Anethesia, Auctth eaia
ice13 Lsottrauce) Xylazauet Felanune | Xytaime+ Celanar
Haleitioie4(mgd 16omg urm)C16mgt6omgimlip)

Rat 14 Yyleriuue+ Kdanine xylovnet Lelan

(smg+16omg um) |(16mgt60onmgumlir)
A

A
aafulaoe eriwe 4+tanmelaue+ölauim
M

Cuiuea p1
(ualalion) (2mgt 8omg um)(umg+1omgu
R

R
A

Rabbiy Jaatulanauce Xyloines kolanuze Xylazie+ kelari

H

(uinalatian) (Smg+IS 30mg ym)Smg +tong.im)

P

P
L

L
L
E

E
W

W
E

E
R

R
A

A
C

C
A

A
M

M
R

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Date. ** *
A

A
Expt. N0.. Page N0.
M

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R

R
Experimut uo:a10p
A

A
H

H
P

P
im:> To tielyue blod amle calleTlin uuAmal Aab-
L

L
OraonN OLLu mals.

L
E

E
W

W
Refereuce Para Surumau, R. Raveccelrau aud R. vera-Nad lacH
E

E
Aample callectiau u mallaborator aumal,20t
R

R
A

A
Requirema: Auúnmal,_auaothetic aqeuts cla gloVeSur
C

C
qical hlêde blmd sample Callecliou_uubes veedlo
Capillarube.

geueral_priuiple of blood Callectiu

of bloocd Coleclioushould be discibed u
A

A
Te nieuUMocl
approved by ue Luaitute_ auinnal ptlic
M

he pratoca
R

Comnuttee.
A

ILAuould Jeartpaulul
he aud siresrfull.Rlocd ample
H

may be coleted uder auaetuLia.

P

Aclequatetraiuiuq Fequired for hlod circulaticm uiug-

L

aUy_nmeiLocd J awyApeuies.
L

uuthdrouiu trom veuou art-

E

E
Tugeueral blmcl Aompleds
W

eia blmd veueu ou ueut cluamber. W

E

E
CareSuould be talce fo alder aud obeie auina.
R

R
A

A
C

Procedure:
Afor dlorsal Pedal Veiu:

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Datc
A

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Expt. No..
M

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Page No.-
R

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A

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1.Tue auinual u lecep ul a relraJne.
H

H
P

P
2Hald the luud fat around Gele+he medial clorial_pedal veua
L

L
u dotarecl ou tap of the fool..

L
3). Cleauthe Foot ulith_abialule alcholuol

E
auud eutdueLe_clorAdS-
W

W
pedal veiw _uutlk 23Cr I2
CueRdle
E

E
R

R
BforTau vuiu:s
A

A
C

C
1| Cepl
eouumal u_restraiuer, tenperalure [2uto 21°C
2 DoT6 Hub heTailit MCLy_resull uu_leukocxtais Tt Hhe
Veiu d Co vuible heu dip the tail u_wam watur[uo'c
3 Kefore the 3onivntey of expurinioutal apply Jocal auacttioticC
Cream ou tail.
u). uAer ue 23 uee dile unto_blod Veuet auedcollect ttesampte
A

A
capillary tuhe.
M

ing
5 asu tlueeraiuer to avoid CrO yeticuu.
R

R
A

A
H

Fortail Suip Louly for nuicel

P

P
L

.Apply Local auaciheuia ou tal.

L

a
E

E
2). Make cul of 1 mm froMipof lail_Acadpel blede.
W

3) Callectue s0m(le. W
E

E
u) top the blmd fAlow by dabbiug? uedau dip
R

R
A

A
C

Result :4The blod Aample Callecli uAMall JaboratorY

auinal uuk siudied success.tull.

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