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GMF For CRB

The document discusses the production and application of green muscardine fungus (GMF) to manage coconut rhinoceros beetles. GMF is cultured on potato dextrose agar and corn grits, harvested when fully sporulated, then dried and packaged. It is applied to artificial breeding sites like logs filled with decomposing matter. GMF spores infect the beetles, multiplying inside and emerging to cause mummification within 10 days. Log traps are monitored over time to assess fungal effectiveness against the beetle pest.

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Vincent Tacda
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135 views

GMF For CRB

The document discusses the production and application of green muscardine fungus (GMF) to manage coconut rhinoceros beetles. GMF is cultured on potato dextrose agar and corn grits, harvested when fully sporulated, then dried and packaged. It is applied to artificial breeding sites like logs filled with decomposing matter. GMF spores infect the beetles, multiplying inside and emerging to cause mummification within 10 days. Log traps are monitored over time to assess fungal effectiveness against the beetle pest.

Uploaded by

Vincent Tacda
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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Green Muscardine Fungus Laboratory production of GMF

(Metarrhizium anisopliae)
GMF is cultured in the laboratory in
potato dextrose agar and inoculated to corn
The green muscardine fungus (GMF) is a grits for further sporulation. Once media is
naturally occurring entomopathogen that has many fully coated and has turned dark green, the
strains and can be used to manage many insect pest fungus is harvested, dried and packed.
species. One particular strain of GMF can cause
mortality in larval, pupal and adult stages of the
coconut rhinoceros beetle (Oryctes rhinoceros L.).

Four to five log traps per hectare are positioned


along the edges and center of the plantation or
near breeding areas.

Once in contact, the conidia of the fungus will


germinate on the host beetle and penetrate its
cuticle. It will multiply in the hemocoel and other
tissues causing mummification. A white mass of
fungus first appears on the surface of the
mummified body of the beetle about 10 days from
infection. It turns to green after 3 to 5 days. If coconut stumps, saw dusts and fallen logs
abound in the area and are starting to decompose,
Method of application GMF can be applied directly to these breeding
places. The media must be moist enough to
encourage fungal growth.
An artificial breeding site is made by
putting together four, cut, one meter coconut
logs. The cavity is filled to the brim with Monitoring of log traps
sawdust, coco peat, dry animal dung and
other decomposed matter. At least one Log traps can be inspected two months after
hundred grams (100g) of dried GMF is set up to determine if the beetle bred on the trap.
placed in two layers within the sawdust Infected larvae may be transferred to other log
mixture. The top is watered and covered traps to reinforce existing inoculum. The trap
with coconut fronds or banana leaves to maybe added with GMF or decomposed matter if
maintain moisture and encourage fungal needed.
growth.
Prevention of Beetle Infestation Caution ICPD TECHNOGUIDE NO. 1
 Collect and utilize coconut debris like slabs, GMF is not toxic to non-target organisms and Series of 2018
leaves, rachis, cabbage to avoid biomass humans but may cause allergy to sensitive
piling individuals.
 Scatter thinly decomposed matter on the GMF for rhinoceros beetles might not be

ground as fertilizer
Plant covercrops if intercropping is not
effective if used against other insect pests. GREEN MUSCARDINE FUNGUS
practiced (GMF) FOR MANAGEMENT OF
 Practice farm sanitation For more information, please write or call: COCONUT RHINOCEROS
Integrated Crop Protection Division BEETLES
Alternate Hosts of Rhinoceros beetle
DAVAO RESEARCH CENTER
 Agave sisalana (sisal hemp)
 Ananas comosus (pineapple) Philippine Coconut Authority
 Areca catechu (betelnut palm) Department of Agriculture
 Carica papaya (pawpaw) Bago-Oshiro, Tugbok District, Davao City
 Colocasia esculenta (taro) Tel. No. (082) 293-0113, 0115
 Elaeis guineensis (African oil palm)
Fax No. (082) 293-0571
 Lantana
 Metroxylon sagu (sago palm) E-mail : [email protected]
 Musa x paradisiaca (plantain)
 Pandanus (screw-pine)
 Phoenix dactylifera (date-palm)
 Saccharum officinarum (sugarcane)
 Wodyetia bifurcata (foxtail palm)

Other Possible Breeding Sites of Rhinoceros


Beetle

 Corn cobs
 Rice straw
 Cow manure
 Rubber stumps/logs
 Sugar cane bagasse
 Saw dusts
 Banana trunks

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