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CRP Test: A Quick Guide

The CRP Latex test is used to qualitatively and semi-quantitatively detect C-Reactive Protein (CRP) in serum. It uses latex particles coated with anti-CRP antibodies that will agglutinate in the presence of CRP. A positive result indicates a CRP level of ≥ 6 mg/L. The test can also be run quantitatively by performing serial dilutions of the serum sample and determining the highest dilution that still causes agglutination, allowing approximation of the actual CRP concentration. Quality controls with positive and negative sera should be included with each test run.

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167 views3 pages

CRP Test: A Quick Guide

The CRP Latex test is used to qualitatively and semi-quantitatively detect C-Reactive Protein (CRP) in serum. It uses latex particles coated with anti-CRP antibodies that will agglutinate in the presence of CRP. A positive result indicates a CRP level of ≥ 6 mg/L. The test can also be run quantitatively by performing serial dilutions of the serum sample and determining the highest dilution that still causes agglutination, allowing approximation of the actual CRP concentration. Quality controls with positive and negative sera should be included with each test run.

Uploaded by

Housam Elswirki
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C-Reactive Protein (CRP) Test

The CRP Latex test is a rapid slide agglutination test for the qualitative
and semi-quantitative detection of C-Reactive Protein in serum. The
reagent containing particles coated with specific anti-human C-Reactive
protein antibodies, agglutinates in the presence of CRP in the patient’s
serum.

PROCEDURE: -

Qualtitative test

1. Bring the test reagents and samples to room temperature.


2. Place 1 drop (50 µL) of the serum under test into one of the circles
on the card. Dispense 1 drop of positive control serum and 1 drop
of negative control serum into two additional circles.
3. Add 1 drop of CRP-Latex Reagent to each circle next to the sample
to be tested.
4. Mix the contents of each circle with a disposable stirrer while
spreading over the entire area enclosed by the ring. Use separate
stirrers for each mixture.
5. Rotate the slide means of a mechanical rotator (100 r.p.m.) for a
period of 2 minutes.

Reading and interruption

1. Examine macroscopically for the presence or absence of clumps or


agglutination within 1 minute
2. Positive results indicate a level of ≥ 6 mg /

Limitation of the procedure

High CRP level over 1500 mg/L may cause false Negative results due to
prozone effect repeat the test with 20 µL sample.

Quantitative test

1. Mix the CRP latex reagent vigorously.


2. Add 50 µL of 9 g/L saline to circles 2,3,4 and 5.
3. Add 50 µL of patient sample to circles 1,2.
4. Mix the saline and sample in circle 2.
5. Transfer 50 µL from circle 2 to the saline in circle 3.
6. Perform seria (dilution) in the same manner until the last circle.
7. Using the stirrer spread the diluted sample over the entire of
each circle starting at circle 5 and working backward to the neat
sample in circle 1.

Quality control

Each run of tests should be validated with appositive and


negative control.

Reading and interruption

- Negative result – no agglutination. This would indicate a level od


CRP below 3 mg/dl.
- Normal level in adult is up to 6 mg/L.
- Positive sera may be tittered. To titrate make serial two- fold
diluent in 9 g/L saline as indicated in the quantitative test
procedure.

For example

The serum titer is defined as the highest dilution showing


macroscopic agglutination.

The approximate CRP concentration in the sample may obtained by


multiplying the titer by the limit of sensitivity 6mg/L.

Dilution CRP mg /L
Neat 6
1:2 12
1:4 24
1:8 48
1:16 96

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