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Bacteria Culturing & Gram Staining

This lesson plan is about bacteria culturing and gram staining. Students will collect bacteria samples from around the school and culture them in petri dishes. After incubating the samples for three days, students will observe the bacteria and determine their shape and elevation. Next, students will gram stain their bacteria to identify whether each sample is gram positive or gram negative under a microscope. The goal is for students to understand how to manipulate microorganisms and learn about their properties, growth, and role in infectious diseases.

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125 views1 page

Bacteria Culturing & Gram Staining

This lesson plan is about bacteria culturing and gram staining. Students will collect bacteria samples from around the school and culture them in petri dishes. After incubating the samples for three days, students will observe the bacteria and determine their shape and elevation. Next, students will gram stain their bacteria to identify whether each sample is gram positive or gram negative under a microscope. The goal is for students to understand how to manipulate microorganisms and learn about their properties, growth, and role in infectious diseases.

Uploaded by

api-662932195
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© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Teacher: Karem Vidaurri Subject: Principles of Biomedical Science Date: 04/17/2023

SLE/Objective Lesson Title Materials


3.1.6 Study and manipulate
microorganisms to
Bacteria Culturing and Gram 

Petri dish (with bacteria)
Q-tips
understand properties Staining  Inoculation loop
(growth and behavior) and  Ethanol
their role in infectious Warmup/Bell Activity  Iodine
diseases Students will look at the bacteria they  Water
Unit have cultured and determine their  Crystal Violet
shape and elevation on their lab  Safrin
Unit 3: Outbreaks and worksheet.  Pipettes
Medical Emergencies
 Microscope Slide
Vocabulary  Safety googles.
- Gram positive  Gloves
-Gram Negative
-Bacteria Proactive
-Petri dish Students will have to gram stain their
-Incubate bacteria to determine what the bacteria
-Bacilis is.
-Cocci
-Spirilla

Activities
Students will be allowed to go around the school collecting bacteria from different areas in the school. They
will swab the desired area with a Q-Tip for 30 seconds, then swab their bacteria on their petri dish and
incubate the bacteria for 3 days.
Students will look at their petri dish and describe/draw the way it looks in their lab notebook. Students will
now go through a step-by-step process on how to gram stain their bacteria. After they gram-stain their
bacteria they will place their slide under a microscope and determine what bacteria is growing around the
school.

Instructional Strategies Assessment


 Students will be in their table groups. Students will have to prepare their microscope slide through gram
 Students will have 10 minutes to go staining and be able to determine the bacteria by looking at it
around the school and swab for bacteria under a microscope.
samples.
 Students will prepare their petri dish and
incubate their bacteria for 3 days.
 Students will observe the bacteria they
cultured and determine the shape and
elevation.
 Students grab some bacteria from their
petri dish with their inoculation loop and
place it on their microscope slide.
 Students will now begin the gram staining
process.

Notes

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