600 Pump

Service Manual

CH-106817 Revision 2 December 2009

© 2009 Thermo Fisher Scientific Inc. All rights reserved.

Accela™, Chromquest™, and Xcalibur™ are trademarks of Thermo Fisher Scientific. Freon™ and Flourinert™ are
registered trademarks of DuPont. Fluorinert™ is a registered trademark of 3M. All other trademarks are the
property of Thermo Fisher Scientific and its subsidiaries.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.

The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.

Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or error-
free and assumes no responsibility and will not be liable for any errors, omissions, damage or loss that might
result from any use of this document, even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a purchaser. This
document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions
of Sale shall govern all conflicting information between the two documents.

Release history: Revision 2, December 2009

For Research Use Only. Not regulated for medical or veterinary diagnostic use by U.S. Federal Drug
Administration or other competent authorities.
 C

Contents

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .v
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi
Safety Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .vi
Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
Good Laboratory Practices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .viii
Keeping Good Records . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .viii

Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Functional Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Hardware Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Status LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Chapter 2 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Installation Checklist. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Unpacking and Inspecting the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Making the Initial Instrument Preparations. . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Connecting the Power Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Connecting the Pump to the Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Connecting the Pump to Other System Modules . . . . . . . . . . . . . . . . . . . . . . . 14
Connecting the Solvent Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Installing the Optional Dynamic Mixer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Installing the Optional Seal Wash Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Setting up Continuous Flushing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Connecting the Pump to the Autosampler. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Configuring the Pump to the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Powering On the Pump. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

Chapter 3 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33

Maintenance Schedule. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Accessing the Liquid Displacement Assembly Components. . . . . . . . . . . . . . . . 35
Replacing the Primary Piston Seals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Replacing the Secondary Piston Seals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Cleaning the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Cleaning the Pistons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Thermo Scientific 600 Pump Service Manual iii

Contents

Cleaning the Instrument Casing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Flushing the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Flushing the Piston Guide Bushings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Purging Air from the Piston Chambers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Cleaning the Displacement Chambers and the Inlet Module. . . . . . . . . . . . . . . 58
Replacing the Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

Chapter 4 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61

Diagnostic Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Troubleshooting Guides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

Chapter 5 Health Check and Calibration Procedures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71

Calibration Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Testing the Pressure Stability and Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Testing the Flow Rate Accuracy and Detecting Leaks . . . . . . . . . . . . . . . . . . . . 79
Performing the Step Gradient Validation Procedure . . . . . . . . . . . . . . . . . . . . . 84
Software Compatibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

Chapter 6 Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91

Main Body . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
Liquid Displacement Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Inlet Valve Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

Chapter 7 Replacement Parts for Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95

Cables and Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
Pump Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Miscellaneous Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98
Preventive Maintenance Kit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99
Degasser Bracket Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
Franklin and San Jose Part Numbers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111

iv 600 Pump Service Manual Thermo Scientific

 P

Preface
This manual provides you with information on how to install, maintain, and troubleshoot the
Thermo Scientific 600 Pump. The Introduction, Installation, Maintenance, Troubleshooting,
and Replacement Parts chapters are also in the customer’s 600 Pump Hardware Manual. The
Health Check and Calibration Procedures and Replacement Parts for Service chapters contain
information intended only for individuals that have been trained on servicing the 600 Pump.

You operate the 600 Pump from a computer that runs Aria™ operating system (OS) (version
1.6.3 or higher), Xcalibur™, or ChromQuest™ data system.

Several Thermo Scientific chromatography systems use the 600 Pump. Each of these systems
has specific pump installation requirements that supersede any installation procedures
described in this manual. Also, procedures for controlling the pump and performing
diagnostic procedures vary depending on the system that controls the pump. Refer to your
system manual for information on using the system features to control the pump.

Related Documentation
In addition to this guide, you will find information related to 600 Pump in the
following documents:
• Aria OS User Guide—Provides information on controlling the 600 Pump using Aria OS
when the pump is installed in an Aria or Transcend™ system.
• Accela 600 Pump Hardware Manual —Provides installation and spare parts information
when the 600 Pump is installed in an Accela™ system.
• Accela Getting Started with ChromQuest Guide—See this guide for information on
controlling the 600 Pump using Chromquest software when the pump is installed in an
Accela system.
• Accela Getting Started with Xcalibur Guide—Provides information on controlling the 600
Pump using Xcalibur software when the pump is installed in an Accela system.

Thermo Scientific 600 Pump Service Manual v

P Preface
Safety and Special Notices

Safety and Special Notices

Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.

Safety and special notices include the following:

CAUTION Highlights hazards to humans, property, or the environment. Each

CAUTION notice is accompanied by an appropriate CAUTION symbol.

CAUTION Highlights electric shock related hazards to humans. Each electric shock notice
is accompanied by the international high voltage symbol.

CAUTION Highlights chemical related hazards to humans, property, or the environment.

Each chemical notice is accompanied by the chemical caution symbol.

Note Highlights information of general interest.

Safety Information
Observe the precautions listed in this section to ensure the safe operation and longevity of the
instrument.

CAUTION When you use the 600 Pump, follow the generally accepted procedures for
quality control and method development. If you use the 600 Pump in the field of
chromatographic analysis and you observe a change in retention time of a particular
compound, in the resolution between two compounds, or in peak shape, immediately
determine the reason for the changes. Until you determine the cause of the change, do not
rely on separation results.

vi 600 Pump Service Manual Thermo Scientific

 P Preface
Environmental Conditions

CAUTION Use equipment only in the manner specified by its manufacturer, or risk
impairing the protection provided by the equipment.

CAUTION Do not service any part when operating the instrument. Do not remove the
front panel when the instrument is running. Do not run the instrument without the front
panel.

CAUTION The instrument contains voltage lines. Switch off the power and disconnect
the power cable prior to servicing the instrument. There is no need to open the pump
housing as all user serviceable components are outside of the instrument.

CAUTION Never try to repair or replace any components of the instrument not described
in this manual without the assistance of a Thermo Fisher Scientific representative. There
are no operator serviceable parts inside the power supply of the 600 Pump.

CAUTION To prevent personal injury, observe good laboratory practice when handling
solvents, changing tube lines. or both. Consult the pertinent material safety data sheets
(MSDSs) for the solvents used for HPLC analysis.

Environmental Conditions
The installation category (over voltage category) for this instrument is Category 2, which
pertains to equipment that receives its electrical power from the local level, such as an
electrical wall outlet.

The pollution degree for this instrument is Pollution Category 2 (Laboratory): Normally only
nonconductive pollution occurs. Expect temporary conductivity caused by condensation.

Observe the following environmental specifications.

CAUTION Only use the pump according to the conditions stated, or risk personal injury
or damage to the pump.

Thermo Scientific 600 Pump Service Manual vii

P Preface
Good Laboratory Practices

Table 1. Environmental specifications

Environmental Condition Recommendation
Location Indoor use only
Altitude Up to 2000 meters
Temperature 5 °C–40 °C
Maximum relative humidity 80% for temperatures up to 31 °C decreasing linearly
to 50% relative humidity at 40 °C
Voltage fluctuation limit Not to exceed ± 10% of the nominal voltage

Good Laboratory Practices

To obtain optimal performance from your LC system and to prevent personal injury or injury
to the environment, do the following:
• Keep good records.
• Read the manufacturers’ MSDSs for the chemicals you use in your laboratory.
• Remove particulate matter from your samples before you inject them into the liquid
chromatograph.
• Use HPLC-grade solvents.
• Connect the drainage tubes from the pump, autosampler, and detector to an appropriate
waste receptacle. Dispose of solvents as specified by local regulations.

Keeping Good Records

To help identify and isolate problems with either your equipment or your methodology, keep
good records of all system conditions (for example, % RSDs on retention times and peak
areas, peak shape, and resolution). At a minimum, keep a chromatogram of a typical sample
and standard mixture, well documented with system conditions, for future reference. Careful
comparison of retention times, peak shapes, peak sensitivity, and baseline noise can provide
valuable clues to identifying and solving future problems.

Chemical Toxicity
Although the large volume of toxic and flammable solvents used and stored in laboratories can
be quite dangerous, do not ignore the potential hazards posed by your samples. Take special
care to read and follow all precautions that ensure proper ventilation, storage, handling, and
disposal of both solvents and samples. Become familiar with the toxicity data and potential
hazards associated with all chemicals by referring to the manufacturers’ MSDSs.

viii 600 Pump Service Manual Thermo Scientific

 P Preface
Keeping Good Records

Solvent Requirements
Use HPLC-grade solvents that are free of particulates. Choose a mobile phase that is
compatible with the sample and column you have selected for your separation. Be aware that
some solvents are corrosive to stainless steel.

CAUTION Do not use solvents containing Freon™ and perfluorinated solvents, such as
Fluorinert™, FC-75, FC-40, and Fomblin perfluoro polyether solvents from Ausimont.
They adversely affect the Teflon™ AF degassing membrane.

Solvent Disposal
Make sure you have a solvent waste container or other kind of drain system available at or
below the benchtop level. Most solvents have special disposal requirements prohibiting
disposal directly down a drain. Follow all governmental regulations when disposing of any
chemical.

High-Pressure Systems and Leaks

LC systems operate at high pressures. There is little immediate danger from the high pressures
in an LC system. However, if a leak occurs, correct it as soon as possible. Always wear eye and
skin protection when operating or maintaining an LC system. Always shut down the system
and return it to atmospheric pressure before attempting any maintenance.

Contacting Us
There are several ways to contact Thermo Fisher Scientific for the information you need.

❖ To contact Technical Support

Phone 800-532-4732
Fax 561-688-8731
E-mail [email protected]
Find software updates and utilities to download at www.mssupport.thermo.com.

❖ To contact Customer Service for ordering information

Phone 800-532-4752
Fax 561-688-8731
Web site www.thermofisher.com

Thermo Scientific 600 Pump Service Manual ix

P Preface
Keeping Good Records

x 600 Pump Service Manual Thermo Scientific

 1

Introduction
This chapter provides an introduction to the 600 Pump.

Contents
• Functional Description
• Hardware Components
• Status LEDs
• Specifications

Figure 1. Image of the 600 Pump in Transcend pump modular housing

Thermo Scientific 600 Pump Service Manual 11

1 Introduction
Functional Description

Figure 2. Image of the 600 Pump in a Transcend pump modular housing with covers open

Functional Description
The 600 Pump is a quaternary, low-pressure mixing pump with a built-in solvent degassing
system and an automatic calibration feature. The pumping system provides optimum
performance in the flow rate range of 1 μL/min to 5 mL/min and a maximum operating
pressure of 600 bar. For ultra high performance liquid chomatography systems and
TurboFlow technology applications, the 600 Pump is an excellent choice.

The 600 Pump uses a force sensor feedback controller, which continuously calibrates valve
timing and pumping efficiency based on the measured compressibility of the solvent. This
patent-pending feature enables the 600 Pump to form accurate gradients virtually
pulsation-free; no pulse damping device is required. The force sensor technology measures
system pressure without an additional pressure transducer in the flow path, thereby omitting a
large delay volume that is cumbersome to flush.

The integral vacuum degasser offers superb solvent degassing efficiency using a built-in
vacuum membrane degasser that removes dissolved gasses from the mobile phase.

The 600 Pump, a bench-top unit, is included in several Thermo Scientific systems including
Accela and TLX systems. It uses either a modular pump housing or a system housing. The
pump is remotely controlled with a USB communication link from a computer using Aria
OS, Xcalibur, or ChromQuest software.

12 600 Pump Service Manual Thermo Scientific

 1 Introduction
Hardware Components

Hardware Components
The 600 Pump has these major components: the vacuum degasser assembly, the liquid
displacement assembly (LDA), a 65 μL static mixer, and an optional dynamic mixer. The
pump may also contain status LEDs and an optional seal wash pump for continuously
flushing the piston guide bushings.

Figure 3. Front panel of the Accela Pump.

Static mixer

Purge valve

Vacuum degasser Liquid displacement Dynamic mixer or

assembly assembly outlet adapter

The following topics describe the main components of the 600 Pump:
• Vacuum Degasser Assembly
• Liquid Displacement Assembly (LDA)
• Purge Valve
• Dynamic Mixer
• Seal Wash Pump

Thermo Scientific 600 Pump Service Manual 13

1 Introduction
Hardware Components

Vacuum Degasser Assembly

The 600 Pump contains a built-in vacuum membrane degasser that removes dissolved gasses
from the mobile phase. Dissolved gasses can cause many problems in HPLC and must be kept
to an absolute minimum for best performance. The solvent degassing system consists of four
independent channels that are maintained at a constant vacuum of approximately 50 mm Hg
absolute. Each channel has a volume of less than 500 μL, which provides superior degassing
efficiency capable of limiting dissolved oxygen to a level of only 0.8 ppm. By varying its speed,
the vacuum pump maintains a constant vacuum level. This eliminates the excess baseline
noise and drift caused by vacuum pump cycling.

Liquid Displacement Assembly (LDA)

The frame of the liquid displacement assembly (LDA) consists of two components: the LDA
body and the inlet module. The LDA body contains the inlet and outlet check valves, two
ceramic pistons and their seals, upper and lower piston chambers, and various PEEK seal
rings. The inlet module contains four high-precision proportioning valves.

As the lower piston withdraws, mobile phase from the proportioning valve assembly passes
through the inlet check valve and fills the lower piston chamber. As the lower piston returns,
the inlet check valve closes and the mobile phase is pushed through the outlet check valve and
into the upper piston chamber. Both check valves are low volume and incorporate a dual
ball-and-seat design, which ensures a positive seal with minimal delay volume.

Next, the upper piston moves into the upper piston chamber, closing the outlet check valve
and forcing the mobile phase out the front of the outlet module.

Purge Valve
The purge valve is located to the left of the LDA. See Figure 3. Turning the wingnut
counterclockwise opens the purge valve.

When the purge valve is open and the solvent flow is experiencing back pressure from either
an LC column or a back pressure regulator, mobile phase exits the LDA from the left side of
the outlet module.

When the purge valve is closed, mobile phase flows out the front of the LDA assembly and
into the high-pressure tubing that connects the LDA to the mixer or outlet adapter and the
mixer to the autosampler.

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 1 Introduction
Status LEDs

Dynamic Mixer
Adding the optional dynamic mixer to the 600 Pump ensures mixing of complex or relatively
incompatible solvents and reduces the chromatographic baseline noise for applications using
proportioned mobile phases. The integrated dynamic mixer also increases the accuracy and
reproducibility of complex ternary and quaternary gradients. The dynamic mixer adds 35 μL
of gradient delay volume to the system. You can plumb the system to bypass the dynamic
mixer for mobile phases that do not require additional mixing. 600 Pumps that do not
contain the dynamic mixer have an outlet adapter installed in its place.

Seal Wash Pump

You can add a seal wash pump to the 600 Pump for continuous flushing of the piston guide
bushings. Use the seal wash pump for optimal pump performance when working with highly
concentrated buffer solutions.

As the pistons move back and forth within the displacement chambers, a small quantity of
mobile phase leaks behind the primary piston seals. With buffered mobile phases, a precipitate
(also known as creep or salt build-up) forms as the liquid fraction evaporates. The abrasion
caused by this precipitate can scratch the piston and shorten the life of the piston seals. With
the seal wash pump option, a large reservoir above the pump continuously feeds the pump
and flushes the piston guide bushings to prevent the precipitation of salt crystals behind the
primary piston seals.

Status LEDs
If the 600 Pump is housed in a modular housing, four status LEDs provide information about
the pump. Table 2 describes the four status LEDs.
Table 1. Status LEDs for the 600 Pump
LED Status Description
Power Green The pump is switched on and is receiving power.
Comm Amber The pump is not communicating with the data system PC.
Green Communication to the data system PC has been established.
Flashing green A pump program is downloading from the data system PC.

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1 Introduction
Specifications

Table 1. Status LEDs for the 600 Pump

LED Status Description
Run Amber The power is switched on, but the pump pistons are idle,
producing no flow.
Flashing amber A firmware download is in progress or an error condition has
occurred.
Green The pump pistons are moving, but a pump program is not
running. This can occur when the pump is under direct
control or a pump program has ended.
Flashing green The pump is running a pump program from a downloaded
method.
Degas Amber he degas unit is building vacuum.
Flashing amber A failure, such as a loss of vacuum, has occurred.
Green Sufficient vacuum has developed to perform
chromatography.

Specifications
The tables in this section list the pump’s physical dimensions, pump and degasser operating
specifications, and electronic specifications.
Table 2. Physical dimensions
Specification Description
Height (with tray) 178 mm
Width 355 mm
Depth 457 mm
Weight 13.8 kg

Table 3. Pump operating specifications

Specification Description
Flow rate range 1–5000 μL/min
Back pressure range 0–600 bar
Residual pulsation < 2 bar Amplitude
Flow rate accuracy ±0.5% or 1u/min whichever is greater
Gradient composition accuracy ±0.5% absolute

16 600 Pump Service Manual Thermo Scientific

 1 Introduction
Specifications

Table 3. Pump operating specifications

Specification Description
Gradient resolution 1% from 0 to 100%
Firmware version 2340e
Gradient composition range 0 to 100% from up to 4 independent feed channels
employing 2 of them at the same time
Gradient linearity 0.9999 < r < 1.0001 from 5 to 95%
Delay volume 90μL (LDA) + 65μL (static mixer)

Table 4. Degasser operating specifications

Specification Description
Channels 4
Degassing technology Gas permeation through a fluoropolymer membrane
Maximum flow rate 10 mL/min
Degassing capacity ~2 ppm residual gas at 1 mL/min
Delay volume per channel ~480 μL
Wetted surfaces PEEK, glass-filled PTFE, Teflon™ AF

Table 5. Electronic specifications

Specification Description
Outputs 5 relays (4 programmable), 2 analogue (1 for pressure)
Inputs 2 voltage actuated, 1 analogue
Communications 19200 baud, 1 start, 8 data, 1 stop, no parity and no
handshaking. Proprietary protocol/USB 1.0
Audio output Piezo buzzer

Thermo Scientific 600 Pump Service Manual 17

1 Introduction
Specifications

18 600 Pump Service Manual Thermo Scientific

 2

Installation
This chapter provides instructions for the initial installation of your 600 Pump.
Note Installation procedures vary depending on the instrument control or data system
that controls the 600 Pump. Refer to system installation documentation when applicable.

Contents
• Installation Checklist
• Unpacking and Inspecting the Instrument
• Making the Initial Instrument Preparations
• Connecting the Power Cable
• Connecting the Pump to the Computer
• Connecting the Pump to Other System Modules
• Connecting the Solvent Lines
• Installing the Optional Dynamic Mixer
• Installing the Optional Seal Wash Pump
• Setting up Continuous Flushing
• Connecting the Pump to the Autosampler
• Configuring the Pump to the Software
• Powering On the Pump

Thermo Scientific 600 Pump Service Manual 9

2 Installation
Installation Checklist

Installation Checklist
This section provides a brief summary of the steps that must be completed for the proper
installation of your 600 Pump:

❒ Unpack and inspect your instrument (see “Unpacking and Inspecting the Instrument”
on page 10).
❒ Read safety precautions in “Safety Information” on page vi.
❒ Position the pump appropriately (see “Making the Initial Instrument Preparations” on
page 11).
❒ Make back panel connections (see system installation procedures or “Connecting the
Pump to the Computer” on page 12 and “Connecting the Pump to Other System
Modules” on page 14).
❒ Connect solvent lines (see “Connecting the Solvent Lines” on page 15).
❒ Connect the power cord and turn on the instrument (see “Connecting the Power
Cable” on page 11).
❒ Configure the pump to the instrument control or data system (contact Thermo Fisher
Scientific technical support).

This 600 Pump was installed by:

(Name) (Date)

Unpacking and Inspecting the Instrument

Carefully remove the 600 Pump from the shipping container and inspect the pump and
packing for any signs of damage. If you find any damage, save the shipping materials and
immediately contact the shipping company.

The shipping container contains the items listed in Table 7. Depending on the instrument in
which the pump is used, an accessory kit that includes additional tools and tubing might be
included in the system packaging. Ensure that you have received all the items listed on the
packing list. If any items are missing, contact your Thermo Fisher Scientific field service
engineer immediately.

10 600 Pump Service Manual Thermo Scientific

 2 Installation
Making the Initial Instrument Preparations

Table 7. 600 Pump box components

Description Part number
Accela 600 Pump 50000
Normal tube kit 50015
Packaging 50016
Bag antistatic 50017
Extender tool 50061
FHexagon wrench key with pilot 50084
Documentation

Making the Initial Instrument Preparations

Place the pump on a laboratory bench as close as possible to a designated electrical outlet. Be
sure to place the system in a draft-free location away from an open window, air conditioner
vents, or other circulating air source. A stable room temperature is necessary for applications
requiring maximum detection sensitivity. Choose an area that is free from dust, moisture,
direct sunlight, strong electromagnetic fields, and physical vibrations. Allow at least 15 cm
(6 in.) of clear space between the back panel of the pump and any wall or obstruction. This
provides access to the back-panel connectors and a free flow of cooling air.

CAUTION Never connect the pump to an electrical line source that has power drops or
fluctuations of 10% below the nominal rated line voltage.

Connecting the Power Cable

The AC Adapter supplied with the 600 Pump incorporates a universal AC input switching
regulator. This allows the instrument to operate at any AC line voltage from 100 to 240 VAC
(±10%) with a line frequency range of 47 to 63 Hz. The switching regulator senses the
incoming line voltage and automatically adjusts its operation accordingly.

❖ To connect the pump to line power

1. Connect the power cord to the line power receptacle on the back panel of the pump. See
Figure 4.

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2 Installation
Connecting the Pump to the Computer

Figure 4. 600 Pump back panel showing power receptacle

Power line receptacle

2. Connect the power cord to an appropriate line power source.

Connecting the Pump to the Computer

Pump connections vary depending on the system where you installed the pump. This section
provides general instructions on connecting the USB cord to the pump and computer, and
instructions on connecting the serial cable to the pump and computer. For more information
on connecting the pump to the computer, refer to the specific system assembly instructions or
contact Thermo Fisher Scientific support.

❖ To connect the pump to the computer with a USB cable

1. Connect the series A plug of the USB cable to an available USB port on the computer.
See Figure 5.

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 2 Installation
Connecting the Pump to the Computer

Figure 5. USB plugs and sockets

Series A socket Series B socket

Series A plug Series B plug

2. Connect the series B plug of the USB cable to the USB port on the back of the 600
Pump.
Figure 6. 600 Pump back panel

RS232
connector

USB series B
socket

❖ To connect the pump to the computer using a serial cable

1. Connect one end of the serial communication cable to the pump port marked “RS232”
on the back of the 600 Pump. See Figure 6.
2. Connect the other end of the serial communication cable to the appropriate
communication port on the computer. Using the software, verify that you connected the
cable to the appropriate communication port on the computer.

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2 Installation
Connecting the Pump to Other System Modules

Connecting the Pump to Other System Modules

Depending on the system where you have installed the 600 Pump, you might want to connect
the pump to a valve interface module, autosampler, or another 600 Pump. Use either the
Accela system interconnect cable socket or the D-sub 2-pin connector on the back panel of
the pump to make these connections. Never use both connections.
Note Refer to the specific system assembly instructions or contact Thermo Fisher
Scientific support for more information before making any connections.

See Figure 7 for a description of the back panel connections. Table 8 and Table 9 describe the
Accela system interconnect cable socket and D-sub 25-pin connector.
Figure 7. Back panel connections
Accela System USB series B
interconnect cable socket
socket

RS232 connector D-sub 25-pin

connector

Table 8. Accela System interconnect cable socket pin out descriptions

Pin Description Pin Description
1 GNV 5 Pump ready
2 6V 6 Inject hold
3 Reserved 7 GND
4 Grad start 8 Reserved

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 2 Installation
Connecting the Solvent Lines

Table 9. D-sub 25 pin connector pin out descriptions

Pin Description Pin Description Pin Description
1 Rel. 1 Input 10 Dig. In2+ 19 Rel. 4 NC
2 Rel. 1 NC 11 Rel. Error In. 20 GND
3 Rel. 2 NO 12 Rel. Error NC 21 GND
4 Rel. 3 Input 13 GND 22 Dig. In1-
5 Rel. 3 NC 14 Rel. 1 NO 23 Dig. In2-
6 Rel. 4 NO 15 Rel. 2 Input 24 Rel. Error NO
7 Pressure Out 16 Rel. 2 NC 25 +5V DC
8 +24V DC 17 Rel. 3 NO
9 Dig. In1+ 18 Rel. 4 Input

Connecting the Solvent Lines

To install the solvent lines, follow these procedures
• Assembling the Solvent Reservoir Bottles
• Connecting the Solvents to the Degasser
• Priming the Degassing Membranes

Assembling the Solvent Reservoir Bottles

❖ To assemble the solvent reservoir bottles
1. Label one of the four solvent reservoir bottle caps “A.”
2. Pass one end of the inlet line through the solvent reservoir bottle cap labeled A.
3. Place a solvent reservoir filter onto the end of the inlet line. See Figure 8.
Note Use only the supplied or recommended reservoir filter: part number A-225 20
μL inlet solvent filter from Upchurch/IDEX.

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2 Installation
Connecting the Solvent Lines

Figure 8. Solvent reservoir cap assembly

Solvent reservoir bottle cap

Solvent reservoir filter

4. Insert the solvent reservoir filter and inlet line into one of the solvent reservoir bottles,
and screw the cap onto the solvent reservoir bottle until it is secure.
Note The cap is a two-piece assembly. The upper section (Figure 8) snaps onto a
threaded section. You can screw the threaded section onto the bottle and snap on the
upper section after installing the tube, or, if you are replacing existing tubing, you can
unscrew the entire cap from the bottle.

5. Position the bottle in the solvent platform, allowing the solvent inlet line to hang down
along the left side of the system.
6. Repeat step 1 through step 5 for solvents B, C, and D if applicable.

Connecting the Solvents to the Degasser

Use HPLC-grade solvents that are free of particulate matter. Always use a solvent filter with a
solvent line sinker frit in the mobile phase reservoirs.

CAUTION Do not use solvents containing Freon and perfluorinated solvents, such as
Fluorinert, FC-75, FC-40, and Fomblin perfluoro polyether solvents from Ausimont.
They adversely affect the Teflon AF degassing membrane.

CAUTION To prevent personal injury, observe good laboratory practice when handling
solvents, changing tube lines, or both. Consult the pertinent material safety data sheets for
the solvents used for HPLC analysis.

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 2 Installation
Connecting the Solvent Lines

❖ To connect the solvents to the degasser

1. Using a PEEK fitting, connect a length of 1/8 inch OD × 1/16 inch ID. Teflon
chromatography tubing from the solvent reservoir to port A on the vacuum degasser inlet.
See Figure 9.
Figure 9. View of pump showing vacuum Degasser inlet ports
Vacuum degasser inlet port A

2. Push a length of tubing through a PEEK 1/8 inch externally threaded ¼-28 fitting and
slide a ferrule over the tubing end. Cut the Teflon tubing so that the end is flat. See
Figure 10.
Figure 10. Tubing with nut and ferrule installed

Ferrule 1/4-28 Nut

3. Screw the ¼-28 fitting onto outlet port A on the vacuum degasser. Connect the other end
of the tubing to inlet valve A on the LDA. See Figure 11.

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2 Installation
Connecting the Solvent Lines

Figure 11. View of pump showing vacuum degasser outlet ports

Degasser outlet Inlet valve A

port A connection

Note Use the supplied extender tool to tighten the plastic connectors (see Figure 12).
Be careful not to over tighten them, or risk damaging the threads.

Figure 12. Tightening plastic connectors using the extender tool

Extender tool

4. Repeat step 1 through step 3 to connect additional solvent lines. Use ports B, C, and D.
5. Using the supplied plugs, plug all unused ports. Press the ports in by hand.
6. Continue with “Priming the Degassing Membranes” on page 19.

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 2 Installation
Connecting the Solvent Lines

Priming the Degassing Membranes

Prime the degassing membranes as part of the initial pump installation procedure.
CAUTION The next procedure primes the degassing membranes by pulling solvent
through a syringe. Do not push the solvent into the degasser. Pushing the solvent might
generate excessive pressure and rupture the degassing membrane. The maximum
recommended pressure on the membrane is 10 Bar.

❖ To prime the degassing membranes

1. Remove the tubing connected to inlet valve A.
Figure 13. 600 Pump front panel showing inlet valve A
Inlet valve A
connection

2. Connect a 10 mL syringe to the end of the tubing you removed in step 1.

3. Draw approximately 5 mL solvent into the syringe.
4. Disconnect the syringe and reconnect the tubing to inlet valve A.
5. Repeat step 1 through step 4 to prime solvents B, C, and D through the degasser if
applicable.

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2 Installation
Installing the Optional Dynamic Mixer

Installing the Optional Dynamic Mixer

An optional 35 ml dynamic mixer is available for all 600 Pumps. See Figure 14. To install the
dynamic mixer, see the product insert provided with the mixer or the following instructions.
Figure 14. View of pump showing the dynamic mixer
Dynamic mixer Dynamic mixer
inlet port outlet port to autosampler

To install the dynamic mixer, you must have these tools:

Tool Use
1/4 in. open-end wrench Disconnect the high-pressure lines
#2 Phillips head screwdriver Disconnect the chassis from the housing
2.5 mm ball driver wrench Disconnect the screws that secure the blind outlet module
or the dynamic mixer to the front panel of the pump

❖ To install the dynamic mixer

1. Turn off the power to the pump.
2. Unplug the pump from line power.

CAUTION To prevent electrical shock, turn off the pump and disconnect it from line
power before you install the dynamic mixer.

3. Using a 1/4 in. open-end wrench, disconnect the static mixer from the blind outlet
module (see Figure 15 and Figure 16).

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 2 Installation
Installing the Optional Dynamic Mixer

4. Disconnect the tubing between the pump and the autosampler, using a 1/4 in. open-end
wrench, disconnect the high-pressure solvent line from the blind outlet module.
Figure 15. View of the static mixer connection to the blind outlet module
Static mixer connection to Blind outlet
the blind outlet module module

Figure 16. View of the static mixer disconnected from the blind outlet module
Static mixer

5. Using a #2 Phillips head screwdriver, remove the screw that secures the metal plate
covering the LED board, and then remove the metal plate (see Figure 17).

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2 Installation
Installing the Optional Dynamic Mixer

Figure 17. View of the pump with the doors open

Screw that secures the Four screws that secure the housing
metal plate (See step 5) to the chassis (See step 7)

6. Disconnect the LED cable from the left door of the pump.
7. Using a #2 Phillips head screwdriver, remove the four screws that secure the pump chassis
to the housing, and then pull the chassis out of the housing (see Figure 17).
8. Using a 2.5 mm hex ball driver, remove the two screws (with washers) that secure the
blind outlet module to the front panel of the pump (see Figure 18). Set the screws and
washers aside. You use them in step 12 to secure the dynamic mixer to the pump.
Figure 18. View of the back side of the front panel

Screws that secure the blind outlet module to

the front panel of the pump

9. Remove the blind outlet module from the front panel of the pump (see Figure 19).

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 2 Installation
Installing the Optional Dynamic Mixer

Figure 19. View of the blind outlet module being removed from the front panel
Blind outlet module

10. Using a 1/4 in. open-end wrench, disconnect the static mixer from the LDA
(see Figure 20).
Figure 20. view of the connection between the LDA and the static mixer
Connection between LDA and
static mixer

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2 Installation
Installing the Optional Dynamic Mixer

11. Insert the dynamic mixer into the port vacated by the blind outlet module
(see Figure 21).
Figure 21. View of the dynamic mixer inserted into the port vacated by the blind outlet
module
Dynamic mixer

12. From the back side of the front panel (see Figure 22), secure the dynamic mixer with the
screws that you removed in step 8 on page 22.
Figure 22. View of the dynamic mixer from inside the pump chassis

Screws that
secure the
dynamic mixer
to the pump

13. Using a 2.5 mm hex ball driver, tighten the screws that secure the dynamic mixer to the
pump (see Figure 22).

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 2 Installation
Installing the Optional Dynamic Mixer

14. Push the pump chassis back into the housing, and then secure the chassis to the housing
with the four screws that you removed in step 7 on page 22.
15. Reconnect the LED cable to the LED board.
16. Reconnect the metal plate that covers the LED board. Using a #2 Phillips head
screwdriver, secure the metal plate to the left door of the pump.
17. Reinstall the static mixer as follows:
a. Using a 1/4 in. open-end wrench, reconnect the static mixer to the LDA.
b. Using a 1/4 in. open-end wrench, connect the static mixer to the inlet of the dynamic
mixer (see Figure 23).
Figure 23. View of the static mixer connected to the LDA and the dynamic mixer
LDA outlet Static mixer Dynamic mixer inlet

18. Connect the outlet of the dynamic mixer to the inlet of the autosampler’s heat exchanger
(see “Connecting the Pump to the Autosampler” on page 31).

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2 Installation
Installing the Optional Seal Wash Pump

Installing the Optional Seal Wash Pump

If your applications use highly buffered mobile phases, Thermo Fisher Scientific recommends
that you install the optional seal wash pump.

To install the optional seal wash pump, you must have these tools:

Tool Use
#2 Phillips head screwdriver Disconnect the LED cover plate from the inside of the
pump’s left door and the chassis from the housing
2.5 mm hex ball driver Disconnect the screws that secure the cover plate to the
front panel and secure the seal wash pump to the front
panel

❖ To install the optional seal wash pump

1. Turn off the power to the pump.
2. Unplug the pump from line power.
3. Using a 2.5 mm hex ball driver, remove the two screws that secure the blind cover to the
front panel of the pump (see Figure 24). Set the screws aside. Use these screws to secure
the seal wash pump to the front panel of the pump.
Figure 24. View of the blind cover removed from the pump

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 2 Installation
Installing the Optional Seal Wash Pump

4. With the cable connector facing up (see Figure 25), slide the seal wash pump into the
front panel of the pump (see Figure 26).
Figure 25. Seal wash pump
Cable connector

Figure 26. View of the seal wash pump placed into the front panel of the pump

Seal wash pump

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2 Installation
Installing the Optional Seal Wash Pump

5. Using the screws that you removed in step 3 on page 26, secure the seal wash pump to the
front panel of the pump (see Figure 27).
Figure 27. View of the seal wash pump secured to the front panel of the pump
Screws that secure the seal wash pump

6. Using a 2.5 mm hex ball driver, tighten the screws.

CAUTION To prevent electrical shock, turn off the pump and disconnect it from line
power before you install the seal wash pump.

7. Using a #2 Phillips head screwdriver, remove the screw that secures the metal plate
covering the LED board, and then remove the metal plate (see Figure 17 on page 22).
8. Disconnect the LED cable from the left door of the pump.
9. Using a #2 Phillips head screwdriver, remove the four screws that secure the pump chassis
to the housing, and then pull the chassis out of the housing (see Figure 17).

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 2 Installation
Installing the Optional Seal Wash Pump

10. Using the cable supplied with the seal wash pump, connect the seal wash pump to
Valve E on the Controller PCB (see Figure 28).
Figure 28. View of the connection between the seal wash pump and the Controller PCB
Cable connection to
seal wash pump

Cable connection to
Controller board

11. Push the pump chassis back into the housing, and then secure the chassis to the housing
with the four screws that you removed in step 9 on page 28.
12. Reconnect the LED cable to the LED board.
13. Reconnect the metal plate that covers the LED board. Using a #2 Phillips head
screwdriver, secure the metal plate to the left door of the pump.
14. Remove the inner wash line from the guide bushings (see Figure 29).

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2 Installation
Setting up Continuous Flushing

Figure 29. View of the guide bushings with the inner wash line removed
Inner wash line removed

15. To set up the seal wash pump for continuous flushing, go to the next procedure.

Setting up Continuous Flushing

If your pump is equipped with a seal wash pump, you can set up continuous flushing of the
piston guide bushings. This is recommended if you use mobile phases with a high
concentration of salts. For the following procedure, see Figure 30.

❖ To set up continuous flushing

1. Connect a reservoir of distilled water to the wash pump inlet.
2. Connect the upper wash tubing of the lower guide bushing to the wash pump outlet.
3. Connect the lower wash tubing of the upper guide bushing to a waste reservoir.

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 2 Installation
Connecting the Pump to the Autosampler

Figure 30. View of pump showing wash pump connections

Wash pump Wash pump Connection to
outlet inlet waste

Connecting the Pump to the Autosampler

❖ To connect the 600 Pump to the autosampler
1. Using a high-pressure fitting, connect a length of high-pressure tubing to one of the
following ports:
• Dynamic mixer outlet port if one is installed
• Outlet adapter port

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2 Installation
Configuring the Pump to the Software

Figure 31. Dynamic mixer outlet

Dynamic mixer outlet

2. Connect the other end of the tubing to the appropriate port on the autosampler injection
valve. Contact technical support for information on the autosampler injection valve
connections.

Configuring the Pump to the Software

The 600 Pump can be configured to communicate with Aria OS, Xcalibur, and
ChromeQuest software. Contact Thermo Fisher Scientific technical support for more
information on performing this procedure.

Powering On the Pump

❖ To turn on the pump
1. Verify that the power switch at the front of the unit is in the Off position.
2. Connect the power cord. See “Connecting the Power Cable” on page 11.
3. Connect the pump to the computer. See “Connecting the Pump to the Computer” on
page 12.
4. Connect the solvent lines. See “Connecting the Solvent Lines” on page 15.
5. Connect the pump to the autosampler. See “Connecting the Pump to the Autosampler”
on page 31.
6. Press the power On button. If your pump has status LEDs, the Power LED turns solid
green.
7. Configure the pump to the software. See “Configuring the Pump to the Software” on
page 32.

32 600 Pump Service Manual Thermo Scientific

 3

Maintenance
To perform preventive and corrective maintenance procedures for the 600 Pump, you must
have access its components. This chapter also describes how to remove and dismantle the
LDA and basic maintenance procedures.

CAUTION Read all safety precautions before performing any maintenance procedures on
the 600 Pump. See “Safety Information” on page vi.

CAUTION The instrument contains voltage lines. Switch off the power and disconnect
the power cable prior to servicing the instrument. There is no need to open the pump
housing, however, as all user serviceable components are outside of the instrument.

Contents
• Maintenance Schedule
• Accessing the Liquid Displacement Assembly Components
• Replacing the Primary Piston Seals
• Replacing the Secondary Piston Seals
• Cleaning the Check Valves
• Cleaning the Pistons
• Cleaning the Instrument Casing
• Flushing the Pump
• Flushing the Piston Guide Bushings
• Purging Air from the Piston Chambers
• Cleaning the Displacement Chambers and the Inlet Module
• Replacing the Fuses

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3 Maintenance
Maintenance Schedule

Maintenance Schedule
To ensure the 600 Pump functions properly, perform the preventive maintenance procedures
at regularly scheduled intervals. Perform the corrective maintenance procedures when you
observe poor performance or when you have been instructed to do so by a Thermo Fisher
Scientific service representative.

Table 10 lists the minimum recommended frequencies for performing the maintenance
procedures described in this manual. However, the volume and type of mobile phases that
flow through the pump affect the how often you should perform these procedures. If
warranted, increase the frequency of these procedures.
Table 10. Maintenance frequency
Procedure Frequency
Replacing the Primary Piston Seals Every 6 to 12 months depending on usage
Replacing the Secondary Piston Seals Every 6 to 12 months depending on usage
Cleaning the Check Valves Every 6 to 12 months if you use buffered mobile
phases
Cleaning the Pistons Every 6 to 12 months depending on usage
Cleaning the Instrument Casing As needed
Flushing the Pump Once per day if you use buffered mobile phases
and you do not have the seal wash pump installed
Flushing the Piston Guide Bushings Once per day if you use buffered mobile phases
and you do not have the seal wash pump installed
Purging Air from the Piston Chambers As needed
Cleaning the Displacement Chambers As needed
and the Inlet Module
Replacing the Fuses As needed

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 3 Maintenance
Accessing the Liquid Displacement Assembly Components

Accessing the Liquid Displacement Assembly Components

Perform the procedures described in this section to access the check valves, piston seals,
displacement chambers, and outlet module.

Once you have accessed the liquid displacement assembly (LDA) components, perform all the
necessary maintenance procedures that require access to components before reassembling the
LDA. The following procedure require access to the LDA components:
• “Replacing the Secondary Piston Seals” on page 53.
• “Cleaning the Check Valves” on page 55.
• “Cleaning the Pistons” on page 56.
• “Cleaning the Displacement Chambers and the Inlet Module” on page 58.

Removing the Liquid Displacement Assembly

Remove the LDA to access the piston seals, which you replace during regularly scheduled
preventive maintenance.

❖ To remove the liquid displacement assembly

1. Turn off the power to the pump.
2. Unplug all four inlet valves.
Figure 32. View of the pump with the inlet valves connected

Inlet valves

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3 Maintenance
Accessing the Liquid Displacement Assembly Components

3. Disconnect all four solvent lines.

Figure 33. View of the pump with inlet valves removed and solvent lines connected

Solvent lines

Figure 34. View of the pump with the solvent lines disconnected

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 3 Maintenance
Accessing the Liquid Displacement Assembly Components

4. Using a wrench, unscrew the static mixer at the outlet of the LDA and at the inlet of the
outlet module.
Figure 35. View of the pump with the static mixer

LDA outlet Static mixer Outlet module inlet

5. Using a 2.5 mm hex tool, loosen the two 2.5 M screws on the Z-arm cover and remove
the cover.
Figure 36. View of the pump with the Z-arm cover in place

Z-arm cover M3 2.5 mm screws

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3 Maintenance
Accessing the Liquid Displacement Assembly Components

6. Using a 4mm hex tool, loosen the two clamping screws by three or four turns.
Figure 37. View of the pump showing the two clamping screws

7. Pull out the two piston retaining springs.

Figure 38. View of the pump showing the location of the two piston retaining springs

Piston retaining springs

8. Gently push the piston heads to the left so that they do not contact the Z-arms.

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 3 Maintenance
Accessing the Liquid Displacement Assembly Components

Figure 39. View of the pump with the Z-arms and pistons not touching

Piston head Z-arm

9. Using a 4 mm hex tool, loosen the three LDA attachment screws.

Figure 40. View of the pump showing the location of the LDA attachment screws.

Inlet valve D LDA attachment

screws

CAUTION When loosening the screw near the inlet valves, do not touch or apply
force on inlet valve D to prevent breakage.

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3 Maintenance
Accessing the Liquid Displacement Assembly Components

10. Gently pull the LDA off the two guide pins. Do not let the pistons touch the Z-arms.
Figure 41. Removing the LDA from the pump

Guide pins Piston Z-arm

Dismantling the Liquid Displacement Assembly

Dismantle the LDA as part of the procedure to replace the piston seals or if your Thermo
Fisher Scientific service representative instructed you to do so as part of a corrective or
troubleshooting procedure.

❖ To disassemble the LDA

1. Remove the piston back flush tubes by gently pulling them off.
Figure 42. View of the LDA showing the location of the piston back flush tubes

Piston back
flush tubes

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 3 Maintenance
Accessing the Liquid Displacement Assembly Components

2. Put the LDA onto a level surface. Using the 4 mm hex tool, loosen the fastening screws
that secure the holding hooks until you release the holding hooks.
Figure 43. View of the LDA showing the location of the holding hooks

fastening screws

3. Carefully remove the pistons, guide bushings, and holding hooks from the LDA body.

Figure 44. LDA with the pistons, guide bushings, and holding hooks removed

Holding hook

Piston

Guide
bushings

4. Using the 4 mm hex tool, loosen the compression lid set screw. Remove the compression
lid by sliding it out (see Figure 45).

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3 Maintenance
Accessing the Liquid Displacement Assembly Components

Figure 45. LDA with and without the compression lid

5. Slide out the outlet module, check valves, and displacement chambers.
Figure 46. Sliding out the check valves, displacement chambers, and outlet modules

Check Check Outlet module

valve valve

Displacement Displacement
chamber chamber

Reassembling the Liquid Displacement Assembly

Reassemble the LDA after replacing the piston seals or completing a corrective or
troubleshooting procedure that requires you to disassemble it.

❖ To reassemble the LDA

1. Place the LDA on a clean, level surface.
2. Verify that a check valve is facing the appropriate direction, and insert it into the main
body of the LDA. See Figure 47 and Figure 48.
3. Insert the lower displacement chambers into the main body of the LDA.

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 3 Maintenance
Accessing the Liquid Displacement Assembly Components

4. Verify that the remaining check valve is facing the appropriate direction and insert it into
the main body of the LDA.
5. Insert the upper displacement chamber into the main body of the LDA.
6. Insert the outlet module into the main body of the LDA.
Figure 47. Inserting the displacement chambers, check valves, and outlet module into the LDA

Check Check Outlet module

valve valve

Displacement Displacement
chamber chamber

Figure 48. Check valve orientation

7. Carefully slide the compression lid into the LDA main body (see Figure 49). If the
compression lid does not fit, verify that the parts are installed correctly.

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3 Maintenance
Accessing the Liquid Displacement Assembly Components

Figure 49. Reinstalling the compression lid

8. Using the 4 mm hex tool, firmly tighten the compression lid screw.
Figure 50. Tightening the compression lid screw

9. If you have not already done so, insert the pistons into the holding hook bores and
bushing guides. Align the piston wash tubes so that they protrude away from the holding
hook. See Figure 70.

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 3 Maintenance
Accessing the Liquid Displacement Assembly Components

10. Carefully insert the piston into the bore of the displacement chamber and seal. At the
same time, tighten the holding hook using the 4 mm hex key. Repeat the same procedure
for the second piston unit.
Figure 51. Inserting the piston/guide bushing/holding hook assembly

11. Firmly tighten both holding hooks.

Figure 52. Tightening the holding hooks

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3 Maintenance
Accessing the Liquid Displacement Assembly Components

Reinstalling the Liquid Displacement Assembly onto the Pump

Replace the LDA onto the pump after you have completed the routine or corrective
maintenance procedure that required you to remove it.

❖ To replace the LDA onto the pump

1. Using the 4 mm hex tool, loosen the two clamping screws by three or four turns.
Figure 53. Loosening the two clamping screws

2. Align the pistons in such a way that they do not touch the Z-arms while you insert them.
Do not insert the piston springs at this time.
3. Reinstall the piston back flush tubes.
Figure 54. LDA with the piston back flush tubes reinstalled

Piston back
flush tubes

4. Place the LDA onto the drive unit using the two alignment pins as a guide (see
Figure 55).

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 3 Maintenance
Accessing the Liquid Displacement Assembly Components

Figure 55. Replacing the LDA onto the pump

Alignment pins

5. Tighten the three LDA attachment screws.

CAUTION When tightening the screw near the inlet valve, do not touch or apply
force on inlet valve D.

Figure 56. Tightening the LDA attachment screw near the inlet valve

Inlet valve D LDA attachment screws

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3 Maintenance
Accessing the Liquid Displacement Assembly Components

6. Insert the piston springs. The lip of the spring faces toward the right.
Figure 57. View of the pump with the piston springs installed

Piston retaining springs

7. Using the 4 mm hex tool, tighten the two clamping screws.

Figure 58. Tightening the two clamping screws

8. Replace the Z-arm cover and tighten the screws using a 3 mm hex key (see Figure 59).

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 3 Maintenance
Accessing the Liquid Displacement Assembly Components

Figure 59. View of the pump with the Z-arm cover replaced

M3 2.5 mm
screws

9. Carefully replace the static mixer.

Figure 60. View of the pump with the static mixer replaced

Static mixer

10. Replace the solvent lines (see Figure 61).

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3 Maintenance
Accessing the Liquid Displacement Assembly Components

Figure 61. View of the pump with the solvent lines replaced

Solvent lines

11. Connect the inlet valves.

Figure 62. View of the pump with the inlet valves reconnected

Inlet valves

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 3 Maintenance
Replacing the Primary Piston Seals

Replacing the Primary Piston Seals

Replace the two primary piston seals with new piston seals as part of a regularly scheduled,
preventive maintenance routine or if you detect a leak from the LDA. Thermo Fisher
Scientific suggests replacing the primary piston seals every 6 to 12 months, depending on your
pump usage.

❖ To replace the primary piston seals

1. Remove the LDA from the pump. See “Accessing the Liquid Displacement Assembly
Components” on page 35.
2. Dismantle the LDA. See “Dismantling the Liquid Displacement Assembly” on page 40.
3. Remove the piston from one of the holding hooks and guide bushings.
Figure 63. Piston, holding hook, and guide bushings assembly

Piston

Guide bushings

Holding hook

4. Insert the piston into the primary seal no more than 3 mm. The primary seal is located
inside the displacement chamber.
Figure 64. Displacement chamber with piston inserted

Displacement chamber

Piston

5. Gently remove the seal by pulling on the piston while applying side force. Do not allow
the piston to touch any metal parts.
6. Remove the primary piston seal from the piston and discard the primary piston seal.
7. Insert the piston into the guide bushing and slide the replacement seal over the piston.
Ensure that the replacement seal is flush with the guide bushing and the piston protrudes
out of the seal by 6 mm.

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3 Maintenance
Replacing the Primary Piston Seals

Figure 65. View of piston, guide bushing, and replacement piston seal

piston seal
6 mm

8. Insert the piston into the bore of the displacement chamber. Do not apply any side load
onto the seal during this process.
Figure 66. Using the guide bushing and piston to insert the piston seal

9. Hold down the guide bushing and slide the piston up until it is out of the seal.
Figure 67. Sliding the piston out of the seal

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 3 Maintenance
Replacing the Secondary Piston Seals

10. Remove the guide bushing/piston assembly from the displacement chamber.
Figure 68. Removing the bushing/piston assembly from the displacement chamber

11. Repeat step 1 through step 8 to replace the primary piston seal of the second
displacement chamber with a new piston seal.
12. Continue with one of the following:
• Perform other procedures that require access to the LDA. See “Accessing the Liquid
Displacement Assembly Components” on page 35.
• Rebuild the LDA and replace it onto the pump. See “Reassembling the Liquid
Displacement Assembly” on page 42 and “Reinstalling the Liquid Displacement
Assembly onto the Pump” on page 46.

Replacing the Secondary Piston Seals

Replace the two secondary piston seals as part of a regularly scheduled, preventive
maintenance program or if a Thermo Fisher Scientific representative has instructed you to do
so as part of a corrective or troubleshooting procedure.

❖ To replace the secondary piston seals

1. Remove the LDA from the pump. See “Accessing the Liquid Displacement Assembly
Components” on page 35.
2. Dismantle the LDA. See “Dismantling the Liquid Displacement Assembly” on page 40.
3. Carefully guide the piston through the bore of the holding hook. See Figure 69.
4. Carefully slide a new secondary piston seal over the piston with the spring facing away
from the holding hook.

Thermo Scientific 600 Pump Service Manual 53

 Figure 69. Holding hook with piston and seal

Piston seal spring

5. Remove the secondary piston spring from the piston guide bushing.
6. Carefully slide the piston guide bushing over the piston and align the piston wash tubes
so that they face away from the holding hook.
Figure 70. Proper alignment of the piston wash tubes

Piston wash
tubes

7. Gently push the new secondary piston seal into its groove by pushing the guide bushing
up toward the end of the holding hook.
 3 Maintenance
Cleaning the Check Valves

Figure 71. Holding hook/piston/guide bushing assembly

8. Repeat step 4 through step 6 for the remaining secondary piston seal.
9. Continue with one of the following:
• Perform other procedures that require access to the LDA. See “Accessing the Liquid
Displacement Assembly Components” on page 35.
• Rebuild the LDA and replace it onto the pump. See “Reassembling the Liquid
Displacement Assembly” on page 42 and “Reinstalling the Liquid Displacement
Assembly onto the Pump” on page 46.

Cleaning the Check Valves

If you use buffers with high concentrations of salts, clean the check valves as part of a regularly
scheduled, preventive maintenance procedure every 6 to 12 months depending on your usage.
Clean the check valves if they are not functioning properly or if you experience residual
pulsation that is too high.

CAUTION Do not use an ultrasonic bath to clean the check valves. Sonicating the check
valves can damage their small ruby balls.

❖ To clean the check valves

1. Remove the LDA from the pump body. See “Accessing the Liquid Displacement
Assembly Components” on page 35.
2. Dismantle the LDA. See “Dismantling the Liquid Displacement Assembly” on page 40.
3. Using a 10 mL syringe with a very short piece of silicon tubing at the Luer exit, gently
flush the check valves with 10 mL distilled water.
4. Repeat step 3 with 10 mL of isopropanol.
5. Verify that fluid flows freely through the check valve. If you cannot achieve free flow
through the check valve, replace it with a new one.

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3 Maintenance
Cleaning the Pistons

6. Continue with one of the following:

• Perform other procedures that require access to the LDA. See “Accessing the Liquid
Displacement Assembly Components” on page 35.
• Reassemble the LDA and replace it onto the pump. See “Reassembling the Liquid
Displacement Assembly” on page 42 and “Reinstalling the Liquid Displacement
Assembly onto the Pump” on page 46.

Cleaning the Pistons

Clean the pistons as part of a regularly scheduled preventive maintenance procedure or if you
observe precipitate on them.

❖ To clean the pistons

1. Remove the LDA from the pump body. See “Accessing the Liquid Displacement
Assembly Components” on page 35.
2. Dismantle the LDA. See “Dismantling the Liquid Displacement Assembly” on page 40.
3. Moisten a soft tissue with isopropanol and wipe the pistons with it.
4. Continue with one of the following:
• Perform other procedures that require access to the LDA. See “Accessing the Liquid
Displacement Assembly Components” on page 35.
• Reassemble the LDA and replace it onto the pump. See “Reassembling the Liquid
Displacement Assembly” on page 42 and “Reinstalling the Liquid Displacement
Assembly onto the Pump” on page 46.

Cleaning the Instrument Casing

If the 600 Pump casing becomes dirty, clean it with a soft cloth lightly moistened with a mild
detergent solution. Do not use any type of abrasive pad, scouring powder, or solvent.

Flushing the Pump

If you use buffered mobile phases, flush the pump daily with a non-buffered mobile phase.
This helps prevent particulates from sticking in the check valves, which might cause pressure
problems. To flush the mobile phase through the pump, use your instrument control
software.

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 3 Maintenance
Flushing the Piston Guide Bushings

Flushing the Piston Guide Bushings

As the pistons move back and forth within the displacement chambers, a small quantity of
mobile phase leaks behind the primary piston seals. With buffered mobile phases, a
precipitate (also known as creep or salt build-up) forms as the liquid fraction evaporates. The
abrasion caused by this precipitate can scratch the piston and shorten the life of the
piston seals.

If you use mobile phases that contain a high concentration of salts and you do not have the
seal wash pump, flush the piston seals at least once a day. The seal wash pump is an optional
pump that periodically flushes the piston guide bushings.

❖ To flush the piston guide bushings

1. Connect a 10 ml syringe filled with distilled water to the bottom of the outer tubing.
Figure 72. Syringe connected to the guide bushings for flushing

2. Gently flush the water through the system.

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3 Maintenance
Purging Air from the Piston Chambers

Purging Air from the Piston Chambers

Air trapped in the piston chambers causes an excessive variation in the back pressure. If you
observe excessive variation in the back pressure, follow this procedure to remove the air.

❖ To purge air from the piston chamber

1. Open the purge valve by turning the purge valve wing nut counterclockwise.
Figure 73. View of pump showing purge valve.

Purge valve wing nut

2. Connect a water line to the purge valve.

3. Set the pump flow rate to 1mL/min.
4. Use the instrument control software to pump the water through the LDA for a period of
10 to 15 minutes.
5. Turn off the pump.
6. Close the purge valve.
7. Check the pressure variation to determine if removing the trapped air stabilized the
back pressure.

Cleaning the Displacement Chambers and the Inlet Module

If the primary piston seals have started to decompose and you observe black, fibrous particles
in the chamber, clean the displacement chambers.

❖ To clean the displacement chambers and the inlet module

1. Remove the liquid displacement assembly (LDA) from the pump. See “Accessing the
Liquid Displacement Assembly Components” on page 35.

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 3 Maintenance
Cleaning the Displacement Chambers and the Inlet Module

2. Dismantle the LDA. See “Dismantling the Liquid Displacement Assembly” on page 40.
3. Pry the piston seals out of the bores of the displacement chambers:
a. Carefully insert a piston into the hole in the center of the seal without pushing the
piston further into the bore of the displacement chamber.
b. Gently pry the seal out of the bore.
4. Using a needle, graphite pencil, or equivalent tool, carefully remove the PEEK film seals.
Be careful not to scratch the displacement chambers as you remove the film seals, as this
might cause leaking.
Note Both ends of the lower displacement chamber, the lower end of the upper
displacement chamber, and the bottom of the outlet module contain a PEEK film seal
for a total of four film seals.

5. Place the displacement chambers and the inlet module in a container of water and place
the container in an ultrasonic bath for 10 minutes.
6. Place the displacement chamber and the inlet module in a container of acetone and place
the container in an ultrasonic bath for 10 minutes.
7. Install the PEEK film seals if they are not damaged; otherwise replace them with
new ones.
8. Replace the primary and secondary piston seals. See “Replacing the Primary Piston Seals”
on page 51 and “Replacing the Secondary Piston Seals” on page 53.
9. Continue with one of the following:
• Perform other procedures that require access to the LDA. See “Accessing the Liquid
Displacement Assembly Components” on page 35.
• Reassemble the LDA and place it onto the pump. See “Reassembling the Liquid
Displacement Assembly” on page 42 and “Reinstalling the Liquid Displacement
Assembly onto the Pump” on page 46.

Thermo Scientific 600 Pump Service Manual 59

3 Maintenance
Replacing the Fuses

Replacing the Fuses

Replace the pump fuses if you suspect that the current fuses are not functioning properly.

❖ To replace the fuses

1. Unplug the pump from the power outlet.
2. Using a flat head screwdriver, push the fuse holder lock to the right and pull out the
fuse box.
Figure 74. Pump back panel showing fuse holder

Fuse holder

Fuse holder lock

3. Remove the fuses and replace with 5×20mm, 2A, 250V, TL, slo-blo fuses.
4. Plug the pump power cord into the power outlet.

60 600 Pump Service Manual Thermo Scientific

 4

Troubleshooting
This chapter provides procedures for several diagnostic tests and troubleshooting guides for
the pump and degasser.

Contents
• Diagnostic Tests
• Error Messages
• Troubleshooting Guides

Diagnostic Tests
Run the tests in this section if you want to validate the pump’s performance or if you suspect
the pump is not functioning properly.

Testing the Flow Rate

Run this test to compare the actual flow rate with the flow rate you assigned to the pump.

❖ To measure the actual flow rate

1. Connect a 40 bar restrictor cartridge to the LDA outlet. Align the restrictor so that the
flow is in the direction of the arrow marked on the restrictor.

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4 Troubleshooting
Diagnostic Tests

Figure 75. LDA attached to a restrictor

LDA outlet restrictor

2. Fill a mobile phase reservoir with HPLC-grade methanol and connect it to inlet
channel A.
3. Use the instrument control system to set channel A to 100%.
4. Set the flow rate to 1mL/min.
5. Do one of the following:
• Connect the other end of the restrictor cartridge to an instrument that measures flow
rate.
• Connect the other end of the restrictor cartridge to a length of tubing and place the
tubing into an empty, 10 ml graduated cylinder.
6. Start the pump.
7. Wait 2 minutes.
8. Stop the pump.
9. If you do not have a flow rate meter, measure the fluid in the graduated cylinder. The
actual flow rate equals the measured volume divided by 2.
10. Compare the actual flow rate with the set flow rate. Under these conditions, the accuracy
of the flow should be ±0.5%.

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 4 Troubleshooting
Diagnostic Tests

11. If your flow rate is unstable or inaccurate, see Table 11.

Table 11. Reasons for unstable flow rates
Possible reason for unstable flow rate Correction
Damaged or dirty check valve Clean the check valves. See “Cleaning the
Check Valves” on page 55.
Worn out primary piston seals Replace the seals. See “Replacing the Primary
Piston Seals” on page 51.
Unstable load (column) Use a back pressure restrictor or another
column.

Testing the Residual Pulsation

This test measures the pump pulsation.

❖ To measure the pump pulsation

1. Connect a 40 bar restrictor cartridge to the LDA outlet. Align the restrictor so that the
flow is in the direction of the arrow marked on the restrictor.
Figure 76. LDA attached to a restrictor

LDA outlet restrictor

2. Fill a mobile phase reservoir with HPLC-grade methanol and connect it to inlet
channel A.
3. Open the purge valve.
4. Set the pressure unit to bar in the instrument control software (See the instrument
control software manual).
5. Verify that the pressure indicated by the instrument control software is 0.

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4 Troubleshooting
Diagnostic Tests

6. Set channel A to 100%.

7. Set the flow rate to 500 μL/min.
8. Start the pump.
9. Wait 1 minute.
10. Close the purge valve.
11. Wait 5 minutes to allow the pump pressure to equilibrate.
12. Observe the pressure graph for pressure fluctuations. The pressure standard deviation
should be less than 1 bar.

Testing the Pump Proportioning Accuracy

This procedure tests the pump’s ability to accurately proportion the mobile phases to the four
channels.

❖ To test the pump proportioning accuracy

1. Plumb your system to bypass the autosampler. Connect a 40 bar restrictor cartridge to the
LDA outlet.
2. Connect the other end of the cartridge to a UV detector.
Figure 77. LCL attached to a flow restrictor

3. Fill a mobile phase reservoir bottle with HPLC-grade methanol and connect it to inlet
channels A and B.
4. Fill a mobile phase reservoir bottle with HPLC-grade methanol spiked with 0.5% acetone
and connect it to inlet channels C and D.
5. Set each channel to 25%.

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 4 Troubleshooting
Diagnostic Tests

6. Open the purge valve.

7. Set the flow rate to 1000 μL/min and start the pumps.
8. Wait 2 minutes.
9. Close the purge valve.
10. Wait 2 minutes.
11. Set channel A to 100%.
12. Wait until the system is equilibrated (that is until the baseline is stable.
13. Set the detector to zero at 254 nm. Use an analytical flowcell.
14. Stop the pump.
15. Use Table 12 to create a method to run a step gradient.
16. Run the method and acquire the data using a UV detector at 254nm UV.
Table 12. Gradient file for quaternary pumps
Flow Rate
Time %A %B %C %D
(μL/min)
0 100 0 0 0 1000
5 100 0 0 0 1000
10 0 0 0 100 1000
15 0 0 0 100 1000
15.1 20 0 0 80 1000
20 20 0 0 80 1000
20.1 40 0 0 60 1000
25 40 0 0 60 1000
25.1 60 0 0 40 1000
30 60 0 0 40 1000
30.1 80 0 0 20 1000
35 80 0 0 20 1000
35.1 100 0 0 0 1000
40 0 100 0 0 1000
45 0 0 100 0 1000
50 0 0 100 0 1000
50.1 0 20 80 0 1000
55 0 20 80 0 1000

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4 Troubleshooting
Diagnostic Tests

Table 12. Gradient file for quaternary pumps

Flow Rate
Time %A %B %C %D
(μL/min)
55.1 0 40 60 0 1000
60 0 40 60 0 1000
60.1 0 60 40 0 1000
65 0 60 40 0 1000
65.1 0 80 20 0 1000
70 0 80 20 0 1000
70.1 0 100 0 0 1000
75 0 100 0 0 1000

17. Measure the height of the steps from baseline to top.

Figure 78. Step gradient profile for testing compositional accuracy

18. Perform the calculations in Table 13.

Table 13. Step gradient calculation
Step% Calculation
20% ((E [cm] + J [cm])/2)*100)/ (A [cm] + F [cm])/2) = ________________
40% ((D [cm] + I [cm])/2)*100)/ (A [cm] + F [cm])/2)= ________________
60% ((C [cm] + H [cm])/2)*100)/ (A [cm] + F [cm])/2) = _______________
80% ((B [cm] + G [cm])/2)*100)/ (A [cm] + F [cm])/2) = _______________

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 4 Troubleshooting
Error Messages

19. Compare your results for each step gradient with the pass criteria in Table 14.
Table 14. Pass criteria for gradient test.
Gradient forming performance Pass criteria
20% Step Height: _______% 19.5–20.5%
40% Step Height: _______% 39.5–40.5%
60% Step Height: _______% 59.5–60.5%
80% Step Height: _______% 79.5–80.5%
20% Step Height: _______% 19.5–20.5%
40% Step Height: _______% 39.5–41.5%
60% Step Height: _______% 59.5–60.5%
80% Step Height: _______% 79.5–80.5%

20. If any value falls outside the range, call technical support.

Error Messages
Table 15 describes the pump-related error messages that might appear in the system control
software event log or status area.
Table 15. Error messages
Error message Description
Low Pressure Error The system pressure has fallen below the set minimum pressure.
High Pressure Error The system pressure has risen above the set maximum pressure.
Index Error The system expected an index signal that never arrived. Physical
problem with the hardware or connectors: Contact an
authorized dealer.
Motor Error The motor is malfunctioning. Indicates that too much torque is
required to drive the pump accurately.
Position Error The system was unable to maintain accurate positioning of the
motor. Restart the pump.
Pressure Sensor Error The pressure transducer is malfunctioning.
Solenoid Error A proportioning valve’s solenoid has been disconnected or is
defective. Physical problem with the hardware or connectors:
Contact an authorized dealer.

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4 Troubleshooting
Troubleshooting Guides

Troubleshooting Guides
This section provides troubleshooting guides for the pump and degasser.

Pump Troubleshooting Guide

Table 16 describes symptoms you might observe with the pump, possible causes for the
symptom, and corrective actions.

Table 16. Pump Health Check/Troubleshooting Guide

Symptom Possible cause Solution
No response when power is Power cord not properly Place cord correctly.
switched on installed
Defective power cord Replace cord.
Blown fuse Replace the fuse.
No connection to software Communication cable not Re-install the cable.
firmly installed
Defective communication cable Replace the cable.
Wrong or corrupted firmware Download the current
firmware to the pump.
Flow unstable/pulsation Check valve failure Clean/replace the check
valve. See “Cleaning the
Check Valves” on page 55.
Immiscible solvents Test/change the solvent
system.
Insufficient degassing Use helium degassing.
Solvent feed compromised Clogged frit or insufficient
pressure from the solvent
reservoir.
Defective seals Replace the primary piston
seals. See “Replacing the
Primary Piston Seals” on
page 51.
No flow from the pump Valve failure Clean/replace the check
valve. See “Cleaning the
Check Valves” on page 55.
Pressure error Check the pressure limits.

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 4 Troubleshooting
Troubleshooting Guides

Table 16. Pump Health Check/Troubleshooting Guide

Pump will not start or stop Incorrect wiring Check the wiring.
remotely
Incorrect software/gradient Check the method and the
table settings method options.

Degasser Troubleshooting Guide

Table 17 describes symptoms you might observe with the degasser, probable causes of the
symptoms, and corrective actions.
Table 17. Degasser Health Check/Troubleshooting Guide
Symptom Probable cause Solution
Yellow status LED is on Pump is in start up If vacuum pump speed
steadily, pump is running, and phase or system’s continues to rise for an
RPM seems high. degassing demand has extended period of time (as
increased. heard by the pitch of the
stepper motor), it might
indicate a potential fault
condition.
Yellow status LED is flashing Possible leak. Contact your service
approximately 1 second off, 1 representative.
second on. Vacuum pump is
not running.
Yellow status LED is flashing Possible sensor or Contact your service
approximately 2 seconds off, 1 control board fault. representative.
second on. Vacuum pump is
not running.
Power and Vacuum LEDs Due to the design of the Monitor the UV absorbance of
illuminate green, but the pump and degasser, the non-degassed methanol at 215
pump cannot be heard. pump is very silent at nm versus degassed methanol
low RPMs, even though coming through the degasser.
vacuum is good and Proper performance of the
degassing is normal. degasser should decrease the
UV absorbance of the
methanol significantly.

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4 Troubleshooting
Troubleshooting Guides

Table 17. Degasser Health Check/Troubleshooting Guide

Bubbles move through the Loose fitting or fittings Tighten the input and output
output tubing. fittings.
No solvent flow. If a buffer was left in the Prime/purge the pump head.
degasser for some time
after use, it might plug Use a different channel, or
the degasser. connect the channel to a beaker
of the solvent without the
buffer. Draw the solvent
through the channel to dissolve
the buffer. Do not push the
solvent through the channel. If
this flushing action does not
work, contact your service
representative.

70 600 Pump Service Manual Thermo Scientific

 5

Health Check and Calibration Procedures

Perform the procedures in this section only if you have been trained to service the 600 Pump.

Calibration Tools
Use the tools described in the following table when performing the procedures described in
this chapter.
Table 18.
Description Used for... Picture Part number Quantity
Force sensor calibration wizard Force sensor
downloaded from calibration
http://support.flux.ch/milestones/200
9/04/index.html
Set manometer reference Force sensor Flux part 1
calibration number 50098
includes the following items:
• 50090 Manometer Adapter,
• 50092 Manometer Reference
• 50093 Cable Converter RS485
• 50099 Certificate Of Compliance
• 50164 Assembly Manometer
Adapter
Hypersil Gold 30x2.1 5μm Force sensor 1
calibration

Transfer volume calibration wizard Transfer volume

downloaded at calibration
http://support.flux.ch/milestones/200
9/04/index.html

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5 Health Check and Calibration Procedures
Calibration Tools

Table 18.
Description Used for... Picture Part number Quantity
GJC HPLC liquid flowmeter Transfer volume Flux part 1
calibration number 2000,
model number.
5025000

RS232 input/output cable Transfer volume Supplied with 1

calibration all new
flowmeters
Step Gradient Verification Wizard Step gradient
downloaded at verification
http://support.flux.ch/milestones/200
9/04/index.html
Knauer Smartline UV Detector Step gradient Flux part 1
verification number A5055
www.knauer.net

Knauer Analytical Flow Cell 10mm Step gradient Flux part 1

path length verification number A4061

Table 19. Accessories

Manufacturer Part Description Part Number Pcs. Contact information
Vici-Jour PEEK Tubing 1/16" 0.75mm ID, 10m, JR-T-6003-M10 1 www.vici-jour.com/
Green
Vici-Jour Fitting, PEEK, one piece fingertight, long, R-5507-5 1 www.vici-jour.com/
10-32 Set of 5 Pcs.
Vici-Jour Pre-Cut SS Tubing 1/16" 0.13mm ID JR-T-97020 2 www.vici-jour.com/
300mm, Red
Vici-Jour Nut, SS, 1/16", 10-32, Set of 5 Pcs. JR-MZN1-5 1 www.vici-jour.com/
Vici-Jour Ferrule, SS, 1/16" JR-ZF1S6-5 1 www.vici-jour.com/

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 5 Health Check and Calibration Procedures
Testing the Pressure Stability and Accuracy

Table 19. Accessories

Manufacturer Part Description Part Number Pcs. Contact information
Thermo Fisher Column Hypersil Gold 30x2.1; 5um 25005-032130 1 www.thermo.com
Scientific
Upchurch Back pressure Regulator P-455, 1000 PSI p-455 1 www.upchurch.com
Farnell Farnell Null Modem Cable Assembly 1702772 1 www. farnell.com

Table 20. Complete part list

Manufacturer Part Description Part Number Pcs. www
Vici-Jour PEEK Tubing 1/16" 0.75mm ID, 10m, JR-T-6003-M10 1 www.vici-jour.com/
Green
Vici-Jour Fitting, PEEK, one piece fingertight, long, JR-5507-5 1 www.vici-jour.com/
10-32 Set of 5 Pcs.
Vici-Jour Pre-Cut SS Tubing 1/16" 0.13mm ID JR-T-97020 2 www.vici-jour.com/
300mm, Red
Vici-Jour Nut, SS, 1/16", 10-32, Set of 5 Pcs. JR-MZN1-5 1 www.vici-jour.com/
Vici-Jour Ferrule, SS, 1/16" JR-ZF1S6-5 1 www.vici-jour.com/
Thermo Fisher Column Hypersil Gold 30x2.1; 5um 25005-032130 1 www.thermo.com
Upchurch Back pressure Regulator P-455, 1000 PSI p-455 1 www.upchurch.com
Farnell Farnell Null Modem Cable Assembly 1702772 1 www. farnell.com
Flux Keller LEO 1 Manometer Special Version 50092 1
1200 bar, extra RS485 connector
Flux Manometer Adapter, Inlet and Outlet Port 50090 1
for LEO 1 Manometer
Flux RS485 to USB Manometer Cable 50093 1
GJC GJC HPLC Liquid Flow meter, Model Nr. 2000 1 www.gjcinstruments.c
Instruments 5025000 om
Knauer Knauer Smartline UV Detector 200 A5055 1 www.knauer.net
Knauer Knauer Analytical Flow Cell 10mm path length A4061 1 www.knauer.net _

Testing the Pressure Stability and Accuracy

Perform the Thermo Rheos Series Pump Force Sensor Calibration wizard to calibrate force
sensors (pressure signal) and check residual pulsation of the pump.

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5 Health Check and Calibration Procedures
Testing the Pressure Stability and Accuracy

❖ To perform the Thermo Rheos Series Pump Force Sensor Calibration wizard
1. Connect the Accela 600 Pump outlet to the inlet of the Keller LEO 1 Manometer.
Figure 79. Manometer attached to LDA outlet

2. Connect the reference manometer to a USB port of the computer.

3. Connect the reference manometer to the column. Use Hypersil Gold 30x2.1 5um
Thermo PN 25005-032130.
4. Connect the degasser inlet A to a 2-propanol (isopropanol, IPA) reservoir.
5. Connect the pump to the computer using USB communication.
6. Turn on the pump.
7. Start the Thermo Rheos Series Pump Force Sensor Calibration Wizard. The wizard home
page appears.

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 5 Health Check and Calibration Procedures
Testing the Pressure Stability and Accuracy

8. At the wizard home page, click Next. The following window appears.

9. Click on the Pump ID drop down menu, select the pump you want to calibrate, and
click Next. The following window appears.

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5 Health Check and Calibration Procedures
Testing the Pressure Stability and Accuracy

10. Click on the Manometer ID drop down menu, select the manometer you want to use,
and click Next. The following window appears.

11. Enter the following values into the appropriate fields.

Table 21.
Field Value
Flow Rate for 1st data point 500ul/min
Flow Rate for 2nd data point 2000ul/min
Inlet valve A (IPA)
Equilibration time 180s
Acquisition time 60s
Pressure SD 2bar
Min Scaling Coefficient 60%
Max Scaling Coefficient 140%
Pressure Deviation 5%
Minimum Pressure 75

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 5 Health Check and Calibration Procedures
Testing the Pressure Stability and Accuracy

12. Click Next. The following window appears.

13. Unscrew the two M3x40 mm locking screws and remove the Z-arm cover.

Z-arm cover M3 2.5 mm screws

14. Loosen the two clamping screws by three or four turns.

Purge valve

Clamping screws

Piston springs

15. Open the purge valve.

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5 Health Check and Calibration Procedures
Testing the Pressure Stability and Accuracy

16. Remove the two piston springs and click Next. The following window appears.

17. Close the purge valve.

18. Insert both piston springs.
19. Tighten the clamping spring screws.
20. Reinstall the Z-Arm cover and click Next. When the calibration is complete, the
“Calibration successfully completed” message appears.

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 5 Health Check and Calibration Procedures
Testing the Flow Rate Accuracy and Detecting Leaks

21. Click Next.The following window appears.

22. In the Operator name field, type your name and click Next. The following
window appears.

23. Click Finish. The window closes.

Testing the Flow Rate Accuracy and Detecting Leaks

The Thermo Rheos Series Transfer Volume Calibration Wizard calibrates transfer volume
(Flow Rate Accuracy) and checks the pump for leaks.

❖ To perform the transfer volume calibration wizard

1. Connect the 600 Pump outlet to the column. Use Hypersil Gold 30x2.1 5um Thermo
PN 25005-032130.

Thermo Scientific 600 Pump Service Manual 79

5 Health Check and Calibration Procedures
Testing the Flow Rate Accuracy and Detecting Leaks

2. Set the GC Flow meter to Terminal mode by doing the following, or see the GJC
flowmeter manual page 6-9.
a. Disconnect the flow meter power.
b. Unscrew the flow meter cover.
c. Reset Dil Switch 7 to off.

d. Replace the front cover.

3. Connect the column outlet to the GJC flow meter inlet.
Figure 80. LDA outlet attached to column and flow meter

4. Connect the GJC flow meter to the computer (RS232).

5. Connect degasser inlet A to a 2-propanol (isopropanol, IPA) reservoir.
6. Connect the pump to the computer using USB communication.
7. Turn on the pump.
8. Turn on the flow meter.

80 600 Pump Service Manual Thermo Scientific

 5 Health Check and Calibration Procedures
Testing the Flow Rate Accuracy and Detecting Leaks

9. Start the Thermo Rheos Series Transfer Volume Calibration Wizard. The wizard home
page appears.

10. Click Next. The following window appears.

Thermo Scientific 600 Pump Service Manual 81

5 Health Check and Calibration Procedures
Testing the Flow Rate Accuracy and Detecting Leaks

11. Click on the Pump ID drop down menu, select the pump you want to calibrate, and
click Next. The following window appears.

12. Click on the Flow Meter ID drop down menu, select the flow meter you want to use, and
click Next. The following window appears.

13. Enter the following values into the appropriate fields.

Table 22.
Field Entry
Flow rate for 1st data point 250ul/min
Flow rate for second data point 1750ul/min
Measurements per data point 4
Measurements for equilibration 4
Inlet A (IPA)

82 600 Pump Service Manual Thermo Scientific

 5 Health Check and Calibration Procedures
Testing the Flow Rate Accuracy and Detecting Leaks

Table 22.
Field Entry
Pressure SD allowed 5
Flow Rate Precision 0.5%

14. Click Next. The following window appears.

15. Click Next. When the calibration is complete, the “Calibration successfully completed”
message appears.

Thermo Scientific 600 Pump Service Manual 83

5 Health Check and Calibration Procedures
Performing the Step Gradient Validation Procedure

16. Click Next.The following window appears.

17. In the Operator Name field, type your name and click Next. The following
window appears.

18. Click Finish. The window closes.

Performing the Step Gradient Validation Procedure

The Thermo Rheos Series Step Gradient Validation Wizard checks the proportioning valves
and validates gradient step height. To download the wizard, use the following address:
http://support.flux.ch/milestones/2009/04/index.html.

To perform the step gradient validation:

84 600 Pump Service Manual Thermo Scientific

 5 Health Check and Calibration Procedures
Performing the Step Gradient Validation Procedure

1. Connect the Accela 600 Pump Outlet to the back pressure restrictor. Use restrictor type
1000 PSI Upchurch P-455.

2. Connect the Column Outlet to the Knaur K-200 inlet.

3. Connect the Knaur K-200 detector to the Computer (RS232).
4. Connect Degasser Inlet A and B to Water.
5. Connect Degasser Inlet C and D to Water +0.5% Acetone.
6. Connect the Pump to the Computer using USB communication.
7. Switch on the Pump.
8. Switch on the Knaur Detector.
9. Start the Thermo Rheos Series Step Gradient Validation Wizard. The following window
appears.

Thermo Scientific 600 Pump Service Manual 85

5 Health Check and Calibration Procedures
Performing the Step Gradient Validation Procedure

10. Click Next. The following window appears.

11. In the Pump ID field, select the pump you want to validate and click Next. The following
window appears.

86 600 Pump Service Manual Thermo Scientific

 5 Health Check and Calibration Procedures
Performing the Step Gradient Validation Procedure

12. In the Detector field, select Micro UV Detector K-200 and click Next. The following
window apepars.

13. Enter the following information.

Field Description
Inlet Combination No. 1 Enter the first valve combination to be
tested.
Validate match Select this field if you want to also validate
the second valve pair.
Inlet combination No. 2 Enter the second valve combination to be
tested.
Note This field is only active when
Validate Match has been checked.
Purge all inlets Select this field if you want to purge all
inlets.
Purge time, sec.: Enter the purge time in seconds.
Purge flow rate, μl/min: Enter the purge flow rate in μL/ minute.
Auto zero detector before validation Select this field if you want the wizard to
automatically zero the detector before the
validation. We recommend that you select
this field.
Equilibration time, sec. Enter the equilibration time in seconds.
Acquisition time, sec. Enter the acquisition time in seconds.
Pressure SD, bar Enter the pressure standard deviation limit
in bar.
Max, intensity mAU Enter the maximum intensity limit in
mAU.

Thermo Scientific 600 Pump Service Manual 87

5 Health Check and Calibration Procedures
Performing the Step Gradient Validation Procedure

Field Description
Intensity SD, mAU Enter the intensity standard deviation
limit in mAU.
Step height deviation, % Enter the percent step height deviation
limit.
Intensity drift, mAU Enter the intensity drift limit in mAU.
Correlation Enter the correlation value. The expected
minimum is 0.999.

14. Click Next. The following window appears.

15. Click Next. The following window appears.

88 600 Pump Service Manual Thermo Scientific

 5 Health Check and Calibration Procedures
Software Compatibility

16. Click Next. The following window appears.

17. In the Operator name field, type your name and click Next. The following window
appears.

18. Click Finish. The wizard closes.

Software Compatibility

Table 23.
Pump Type Software Compatibility Firmware
TMO 600 Pump Aria OS 2332e
Accela 600 Xcalibur

Thermo Scientific 600 Pump Service Manual 89

5 Health Check and Calibration Procedures
Software Compatibility

Table 23.
Pump Type Software Compatibility Firmware
Transcend 600 Chromquest
Janeiro II 3.0
Firmware Manager Tool
Pump Identification Wizard
Force Sensor Calibration
Wizard
Transfer Volume Calibration
Wizard
Gradient Validation Wizard

Table 24. TMO 600 Pump

Type Compatibility
Drive unit, cam version/Label on drive unit V72 XXXX (XXXX=Serial Number)
SN range 92000-
Main board version/Label on main board Rheos Controller II - NPG

90 600 Pump Service Manual Thermo Scientific

 6

Replacement Parts
This chapter contains a list of replaceable parts and their corresponding part numbers. To
place an order, contact your local Thermo Fisher Scientific support representative or contact
technical support.

Main Body
The replaceable parts listed in Table 25 correspond to the items listed in Figure 81.
Table 25. Main assembly parts
Item Quantity Description Part number
1 1 Degassing bracket assembly Accela 50038
2 1 Screw ISO 4762 M3×8 50026
3 1 Cover purge pump Accela 600 50025
4 1 LDA Accela 600 50007
5 1 Mixer static Accela 600 50009
6 1 Placeholder Accela 600 50010
7 1 Piston spring stainless steel 20008
8 1 Z-Arm cover Accela 600 50087
9 1 Zyl I-6kt Sch A2/BN 00611 M3×40 90072
10 1 Front panel Accela 600 5022
11 1 Drip tray Accela 600 50027
12 1 Cable valve D Accela 600 50014
13 1 Inlet Module Accela 600 50006
14 1 Cable valve C Accela 600 50013
15 1 Cable valve B Accela 600 50012
16 1 Cable valve A Accela 600 50011

Thermo Scientific 600 Pump Service Manual 91

6 Replacement Parts
Liquid Displacement Assembly

Figure 81. Main pump—front view

(1) Degassing bracket (2) Screw ISO (3) Cover (4) LDA (5) Mixer static
4762 M3×8 purge pump
(6) Placeholder

(7) Piston spring

(8) Z-Arm cover

(9) Zyl I-6kt sch
A2/BN 00611
M3×40

(10) Front panel

(16) Cable valve A (12) Cable valve D

(14) Cable
valve C
(13) Inlet Module (11) Drip tray
(15) Cable valve B

Liquid Displacement Assembly

The replaceable parts listed in Table 26 correspond to the items in Figure 82.
Table 26. LDA Parts
Item Quantity Description Part number
1 1 Outlet module complete Accela 600 50065
2 5 UHP PEEK sealing ring 20095
3 1 Displacement chamber upper 50070
4 2 Check valve gamma titan 10071
5 1 Displacement chamber lower 50069
6 1 UHP LDA main body 10377
7 2 Piston unit (S-finish) ceramic 10004
8 2 Holding hook 50068

92 600 Pump Service Manual Thermo Scientific

 6 Replacement Parts
Liquid Displacement Assembly

Table 26. LDA Parts

Item Quantity Description Part number
9 2 Secondary piston seal PTFE 90002
10 1 Guide bushing 50071
11 2 Primary piston seal GFP 90189
12 1 Adapter LDA Accela 600 50067
13 1 Compression lid including set screw 20002

Figure 82. Liquid displacement assembly

(1) Outlet module complete

(2) UHP PEEK sealing ring

(3) Displacement
chamber upper
(4) Check valve gamma
titan

(5) Displacement
chamber lower

(6) UHP LDA main body

(7) Piston unit

(S-finish) ceramic

(8) Holding hook

(9) Secondary piston seal PTFE

(10) Guide bushing

(13) Compression (12) Adapter LDA (11) Primary
lid including set piston seal

Thermo Scientific 600 Pump Service Manual 93

6 Replacement Parts
Inlet Valve Assembly

Inlet Valve Assembly

The replaceable parts listed in Table 27 correspond to the items in Figure 83.
Table 27. Inlet valve assembly parts
Item Quantity Description Part number
1 1 Flow brake Accela 600 50076
2 1 O-ring 2.69×1.14 50073
3 1 Parts for valve fixation 50079
4 1 Valve Accela 600 50008

Figure 83. Inlet valve assembly

(2) O-ring (3) Parts for

2.69×1.14 valve fixation (4) Valve
(1 ) Flow brake

94 600 Pump Service Manual Thermo Scientific

 Preface
Cables and Connectors

Replacement Parts for Service

The parts listed in this section are intended for individuals that have been trained on servicing
the 600 Pump.

Cables and Connectors

The table in this section lists the cables used on the 600 Pump and their part numbers.
Table 28.
Description Picture Part number Quantity
Intercommunications Cable RS232 10383 1

Cable Valve A Accela 600 50011 1

Cable Valve B Accela 600 50012 1

Cable Valve C Accela 600 50013 1

Cable Valve D Accela 600 50014 1

Thermo Scientific 600 Pump Service Manual 95

Preface
Cables and Connectors

Table 28.
Description Picture Part number Quantity
Connector AC with Cable Accela 600 50029 1

Cable D-Sub25 50083 1

Cable DC Power Accela 600 50048 1

Cable RS232 Accela 600 50050 1

Cable USB Accela 600 50051 1

Cable Interface Board Accela 600 50052 1

96 600 Pump Service Manual Thermo Scientific

 Preface
Pump Components

Table 28.
Description Picture Part number Quantity
Cable Interface Board Degasser Accela 50054 1
600

Cable Valve E Accela 600 50004 1

Witch with Bowden Cable Accela 600 50044 1

Cable DMS Accela 600 50055 2

Pump Components
The components in the following table refer to the items in Figure 84. Other pump
components appear in “Miscellaneous Parts” on page 98 and Chapter 6, “Replacement
Parts.”
Table 29. Pump components
Item number Quantity Description Part number
1 1 Degassing bracket assembly Accela 600 50038
2 1 Tray complete Accela 600 50039
3 1 Drive unit Accela 600 50001
4 1 Controller Accela 600 50002
5 1 UHP LC interface board holder Accela 600 50036
6 1 UHP LC interface board holder Accela 600 50035
7 1 Power supply Accela 600 50045

Thermo Scientific 600 Pump Service Manual 97

Preface
Miscellaneous Parts

Figure 84. Pump component parts for service

(6) UHP LC interface board

holder Accela 600
(7) Power supply
(5) UHP LC interface board Accela 600
holder Accela 600

(4) Controller Accela 600

(3) Drive unit Accela 600

(2) Tray complete Accela 600

(1) Degassing bracket

assembly Accela 600

Miscellaneous Parts
The following table describes components not pictured in Figure 84.
Table 30. Miscellaneous parts
Description Picture Part number Quantity
Static mixer assembly 5009 1

600 Pump extender tool 1

98 600 Pump Service Manual Thermo Scientific

 Preface
Preventive Maintenance Kit

Preventive Maintenance Kit

The parts in the following table are included in the preventive maintenance kit. They refer to
the items shown in Figure 85.
Table 31. Preventive maintenance kit
Item Quantity Description Part number
4 2 Check valve gamma titan 10071
7 2 Piston unit (S-finish) ceramic 10004
9 2 Secondary piston seal PTFE 90002
11 2 Primary piston seal GFP 90189

Thermo Scientific 600 Pump Service Manual 99

Preface
Preventive Maintenance Kit

Figure 85.

(4) Check valve gamma

titan

(7) Piston unit

(S-finish) ceramic

(9) Secondary piston seal PTFE

(13) Compression (11) Primary

lid including set piston seal

100 600 Pump Service Manual Thermo Scientific

 Preface
Degasser Bracket Assembly

Degasser Bracket Assembly

Item Quantity Description Part number

110 1 Board degasser 50102
30 1 Degasser chamber 50103
90 1 Degasser vacuum pump assembly 90002

Thermo Scientific 600 Pump Service Manual 101

Franklin and San Jose Part Numbers

Basel P/N Basel Description SJ P/N SJ Description Franklin P/N

PISTON,1/8,TZP,SURVEYOR MS
10004 Piston unit (S-Finish) ceramic 00201-11324 PUMP,LDA
VALVE,CHECK,SURVEYOR
10071 Check valve (gamma) Titan 00110-05110 MSPUMP,LDA GRADIEN
Proportionating valve (+) Type VALVE,PROPORTIONING,"+"
10088 Press-fitting 00109-03-0004 TYPE MS PUMP CH-953025
Piston Guide Bushing BUSHING, PISTON GUIDE,SRVYR
10097 Titanium/Ruby 00201-11330 MS PUMP,LDA
GEARBOX, MS PUMP, NEW, UP
10112 Rheos 2000-A Drive Unit 00950-01-00077 TO S/N 68073
Rheos Controller I for Rheos PCB,Cont,MS
10121 2000-A 00950-01-00074 Pump,to68073,Servc.Repl.ONLY CH-952940
Quaternary LDA complete, ASSY, LIQUID DISPLACE.W/O
10125 latest revision 00201-11342 COILS,SRVYR
Coil Unit for Proportioning COIL,PROPORTIONING
10158 Valve PEEK/Copper 00201-11344 VALVE,SRVYR MS PUMP CH-952941
Pulse Dampener DAMPENER,PULSE,3ul DEAD
10205 Titanium/Ruby/PEEK 00201-11340 VOLUME,TI,SRVYR
ASSY,PRESSURE SENSOR
10226 Pressure Sensor complete 00201-11338 W/CABLE,SRVYR PUMP CH-952942
10227 M-Pump, OEM unit 60053-30001 PUMP, SRVYR MS,FLUX OEM,PH2
Proportioning Valve Tool
10229 Titanium 60053-01017 TOOL, PROP VALVE MSPUMP
SCREW,NYLON,PROP VALVE
10255 Nylon screw for coil unit 00109-04-00005 COIL, MS PUMP
UHP Version Guide Bushing
10268 Rev. 1.0 (Ti/Ceramic) 00950-01-00127 BUSHING, GUIDE, ACCELA PUMP
Piston UHP version PISTON, SAPPHIRE, REV1.0,
10269 Sapphire/SS Rev. 1.0 00950-01-00126 ACCELA PUMP CH-953026
Proportioning Valve (+) Type VALVE,PROP,MS
10300 1/4 by 28 Fitting 00110-01-00007 PUMP,+TYPE,1/4-28THRD CH-953324
10313 Degassing Bracket Assembly CH-953458
Rheos Controller II for Rheos PCB,Cont,MS
10329 2000-B 00950-01-00075 Pump,68074+,Servic.Repl.ONLY

102 600 Pump Service Manual Thermo Scientific

 Rheos Controller II UHP ASSY, PCB, CONTROLLER II,
10373 Version 00950-01-00132 ACCELA PUMP
UHP Pulse Dampener Module ASSY, PRESS XDCR/DAMPENER,
10374 Titanium/Ruby/PEEK 00950-01-00112 ACCELA PUMP
10377 Main Body LDA UHP LDA Main Body CH-953479
UHP Version Primary piston SEAL, HIGH PRESSURE, GFP,
10382 seal, Fluoroloy 00950-01-00129 ACCELA PUMP CH-953325
CBL
Intercommunications Cable AY,RS232,INTERNAL,DEGASSER
10383 RS232 60157-63004 BD
UHP LDA Rev. 1.0 complete ASSY, LIQUID DISPLACEMENT,
10389 (Sapphire pistons) 00950-01-00111 ACCELA PUMP
GEARBOX, MS PUMP, NEW, S/N
10410 Rheos Drive Unit Version II 00950-01-00079 68074 +
UHP Crossover Tubing Accela TUBE, CROSSOVER, ACCELA
10462 Version 00950-01-00113 PUMP
UHP Drive Unit Version UHP GEARBOX, NEW, V412, REV1.0,
10463 V412 Rev. 1.0 00950-01-00114 ACCELA PUMP
SCREW, SET, Z-ARM/PISTON,
10465 ZHP Z-Arm/Piston Set Screw 00950-01-00115 ACCELA PUMP
UHP LDA Inlet Module MODULE, INLET, LDA, ACCELA
10466 quaternary 00950-01-00119 PUMP
UHP Lower Displacement CHAMBER, DISPLACEMENT,
10467 chamber, Rev. 1.0 00950-01-00121 LOWER,ACCELA PUMP
UHP Upper Displacement CHAMBER,DISPLACEMENT,UPPE
10469 chamber 00950-01-00123 R,ACCELA PUMP
MODULE, OUTLET, ACCELA
10470 UHP Outlet Module complete 00950-01-00124 PUMP
UHP Version Secondary SEAL, WASH, POLYETHYLENE,
10471 Piston seal PTFE 00950-01-00128 ACCELA PUMP CH-953326
10958 Mixer Dynamic 35ul 00109-99-00037
Inlet Check Valve Assy (Dual VLV,CHK, IN, DUAL BALL,
10968 Ball Version) 00950-01-00273 ACCELA PUMP,RoHS CH-953327
Outlet Check Valve Assy (Dual VLV,CHK,OUT,DUAL BALL,
10969 Ball Version) 00950-01-00274 ACCELA PUMP,RoHS CH-953328
Rheos 2000 OEM Accessory KIT,TUBING, No-Ox, SURVEYOR
11026 Kit 00950-01-00021 MS PUMP
External Power Supply for POWER SUPPLY RHEOS 2000
11027 Rheos 2000-B PUMP F CH-952939
15000 Thermo Transcend CH-953303

Thermo Scientific 600 Pump Service Manual 103

 Compression Lid including set LID, COMPRESSION, W/SET SCR,
20002 screw 00950-01-00125 ACCELA PUMP
ELEMENT, BACKING RING,
20007 Backing Element Titanium 00109-03-00002 V1.1,MS PUMP LDA
20008 Piston Spring 00950-01-00116
Wing screw for Valve Spindle, SCREW, WING, PURGE, ACCELA
20013 SS 00950-01-00117 PUMP
RING,SEALING, PROPORTIONING
20083 Sealing ring PTFE 00201-11320 VALVE,SRVYR
SEAL,CUP,PEEK,SRVYR MS
20084 PEEK cup seal 00107-18116 PUMP,LDA GRDNT
20095 UHP PEEK sealing ring 00950-01-10013 SEAL,PACE,PEEK, MS PUMP LDA CH-952744
CARTRIDGE,OUTLET MS PUMP
20096 Outlet cartridge 00109-03-00003 LDA
SEAL,FILM,OUTLET
20120 PEEK sealing ring 00107-18112 CARTRIDGE,SRVYR PUMP"
Displacement Chamber CHAMBER, PISTON,V1.1 MS
20145 revision 1.1 00109-03-00001 PUMP LDA
PTFE Film Seals SEAL,PTFE,PISTON CHAMBER
20223 (Displacement Chamber) 60053-01020 ,MS PUMP LDA
600 Pump
50000 00109-99-00042
Controller Accela 600 PCB, CONTROL, NEW, ACCELA
50002 00950-01-00296 600 PUMP,RoHS
Cable Valve E Accela 600 CBL AY, PROP VALVE, ACCELA
50004 00950-01-00289 600 PUMP,RoHS
Screw ISO 7045 UNF
10-32x3/8" SCW, PN, PH10-32X3/8SS, SEMS,
50005 00415-03206 INT
Inlet Module Accela 600
50006 Inlet Module, Accela 600 CH-953472
LDA Accela 600 ASSY, LDA, ACCELA 600 PUMP,
50007 00950-01-00294 RoHS
Valve Accela 600 VALVE, PROPORTIONING,
50008 00950-01-00285 ACCELA 600 PUMP
Mixer Static Accela 600 MIXER,STATIC, 65uL, ACCELA 600
50009 00950-01-00292 PUMP,RoHS
Placeholder Accela 600 PLACEHOLDER, DM, ACCELA 600
50010 00950-01-00293 PUMP,RoHS

Thermo Scientific 600 Pump Service Manual 104

 Cable Valve A Accela 600
50011 00950-01-00289
Cable Valve B Accela 600
50012 00950-01-00289
Cable Valve C Accela 600
50013 00950-01-00289
Cable Valve D Accela 600
50014 00950-01-00289
Rivet Snap Black RIVET, SNAP, PLK SUPPORT
50021 313018GLIDE
Front Panel Accela 600
50022 Front Panel Accela 600 CH-953460
Nut ISO 4032 UNC 6-32x1/4"
50023 00460-16321 NUT HEX-KEP 6-32, ZP, RoHS
50025 Cover Purge Pump Accela 600 CH-953466
Screw ISO 4762 M3x8
50026 Screw, ISO 4762, M3 X 8 CH-953467
Drip Tray Accela 600
50027 Drip Tray, Accela 600 CH-953459
Screw ISO 7045 UNC
6-32x5/8" SCW, PN, PH6-32x5/8, SEMS, INT,
50028 00415-63215 SS
Connector AC With Cable
Accela 600
50029 60157-63014 CBL AY, AC POWER INTERNAL
FUSEHOLDER,
Fuse Holder Accela 600 DRAWER;5x20mm,2POLE,SAFE,R
50030 2109-0740 oHS
Fuse FUSE, IEC, 2.0A, 250V, SLO-BLO,
50031 5120-0025 5x20mm, RoHS
Screw ISO 7046 UNC
50032 6-32x1/4" 2851-0022 SCR, 6-32X1/4 FHP, BK, SS
Fan Accela 600
50033 60157-63002 CBL, AY, FAN, DIABLO, UHPLC
Screw ISO 7045 UNC 6-32x1
1/4" SCREW, 6-32X1.25, PH, PHIL,
50034 2816-4213 KEP, SS

Thermo Scientific 600 Pump Service Manual 105

 UHP LC Interface Board
Accela 600 ASSY,PCB, UHPLC INTERFACE
50035 60157-61000 BOARD
UHP LC Interface Board
Holder Accela 600 UHP LC Interface Board
50036 60157-10003 Holder,Accela 600 CH-953474
Screw ISO 7045 UNC
6-32x1/4"
50037 00415-63204 SCW PN PH 6-32X1/4 SS SEMS
50039 Tray Complete Accela 600 CH-953478
Switch With Bowden Cable
Accela 600
50044 00302-99-00010 CABLE, BOWDEN, ACCELA
Power Supply Accela 600 PWR SUPPLY, 24V, 2.5A, 60W,
50045 00012-01-00013 ROHS
Cable DC Power Accela 600 CBL AY, UHPLC,PUMP, DC
50048 60157-63000 POWER SUPPLY
Cable RS232 Accela 600 CBL AY,RS232 INTERNAL,
50050 60157-63012 INTERFACE BD
Cable USB Accela 600
50051 00302-99-00009 CBL,USB2.0, A TO B, 12" UL, RoHS
Cable Interface Board Accela
600 CBL AY, COMM AND GRADIENT
50052 60157-63010 LED
Cable Interface Board
Degasser Accela 600
50054 60157-63008 CBL AY, DEGASSER LED
Cable DMS Accela 600 CBL AY, FORCE SENSOR, ACC
50055 00950-01-00290 600 PUMP,RoHS
TOOL, FITTING EXTENDER,
50061 Extender Tool Accela 600 00725-01-00023 ACCELA 600 PUMP
Cover Power Supply Accela
50062 600 00201-99-00105 Enclosure, ASTEC LPS65, RoHS
50063 Mixer Dynamic Accela 600 00950-01-00298 Mixer Dynamic Accela 600
Outlet Module Complete MODULE, OUTLET, ACCELA 600
50065 Accela 600 00950-01-00284 PUMP
50067 Adapter LDA Adapter, LDA, Accela 600 CH-953480
50068 Holding Hook Holding Hook CH-953485

Thermo Scientific 600 Pump Service Manual 106

 CHAMBER,DISP, LWR, ACCELA
50069 Displacement Chamber Lower 00950-01-00281 600 PMP,RoHS
CHAMBER, DISP,UPPER,ACCELA
50070 Displacement Chamber Upper 00950-01-00282 600 PUMP,RoHS
BUSHING, GUIDE, ACCELA 600
50071 Guide Bushing 00950-01-00283 PUMP
O-RING, 2.69mm X 1.14mm,
50073 O-Ring Ø2.69x1.14 00950-01-00286 PERFLUOR
BRAKE, FLOW, INLET MOD,
50076 Flow Brake Accela 600 00950-01-00287 ACCELA 600 PUMP
KIT,PROP VLV ATTACH,ACCELA
50079 Parts For Valve Fixation 00950-01-00288 600 PUMP, RoH
CBL AY,DIGITAL I/O, ACCELA 600
50083 Cable D-Sub25 00950-01-00291 PUMP,RoHS
50086 Screw ISO 4762 M3x4 00605-01-00024
50087 Z-Arm Cover NPG Z-Arm Cover, Accela 600 CH-953462
50101 Set Seal Wash Pump 00960-01-00023 Set Seal Wash Pump
PCB, Degas Controller, Accela (all
50102 Board Degasser 00950-01-00260 Accela family pumps)
Chamber, Vacuum, Mini, Stented
50103 Degasser Chamber 00108-01-00018 (Accela 600 pump and beyond)
Vacuum Pump (used for all Thermo
Degasser Vacuum Pump degassers, regardless of product
50104 Assembly 00960-01-00003 family)
Cable Communication CTC
50157 PAL 00950-01-00299 Cable Communication CTC PAL
GEARBOX, NEW, ACCELA 600
50158 Drive Unit Without Controller 00950-01-00295 PUMP, RoHS
SEAL,PISTON,SECONDARY,SRVY
90002 Secondary piston seal PTFE 00107-18114 R MS PUMP,LDA CH-952742
90052 Eluent filter (PEEK/PTFE) FILTERS ELUENT F CH-952835
Zyl I-6Kt 0 Sch A2/BN 00611 Zyl 1-6Kt 0 Sch A2/BN 00611 M3 X
90072 M3x40 40 CH-953461
SEAL PRIMARY PISTON HIGH
90189 Primary piston seal GFP PERF F CH-952741

Thermo Scientific 600 Pump Service Manual 107

Preface
Franklin and San Jose Part Numbers

108 600 Pump Service Manual Thermo Scientific

 Preface
Franklin and San Jose Part Numbers

Thermo Scientific 600 Pump Service Manual 109

Preface
Franklin and San Jose Part Numbers

110 600 Pump Service Manual Thermo Scientific

 I

Index
A inlet module 58
instrument casing 56
Accela system interconnect cable 14
pistons 56
Accuracy, testing 73
computer, connecting 12
autosampler, connecting 31
connections
low-pressure solvent lines 15
B USB cable 12
back panel Connector AC with Cable Accela 600 96
clearance requirements 11 continuous flushing 5, 30
computer connections 12
connecting the power cables 11 D
connecting to other modules 14
D-sub 25-pin connector 14 degasser
connecting solvents 16
description 2, 4
C priming membranes 16
Cable DC Power Accela 600 96 Troubleshooting Guide 69
Cable DMS Accela 600 97 detecting leaks 79
Cable D-Sub25 96 D-sub 25-pin connector 14
Cable Interface Board Acela 600 96 dynamic mixer 20
Cable Interface Board Degasser Accela 600 97 description 5
Cable RS232 Accela 600 96 installing 20
Cable USB Accela 600 96
Cable Valve A Acceka 600 95 E
Cable Valve B Accela 600 95 electronic specifications 7
Cable Valve C Accela 600 95 environmental conditions vii
Cable Valve D Accela 600 95
Cable Valve E Accela 600 97
cables
F
Accela system interconnect 14 film seals, removing 59
packing location 12, 14 flow rate
USB 12 testing 61
Calibration wizard 74 flow rate, accuracy 79
check valves Force Sensor technology 2
accessing 35 fuses, replacing 58
cleaning 55
checklist, installation 10 H
cleaning
high pressure error 67
check valves 55
displacement chambers 58 Hypersil Gold 30x2.1 5μm 71

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I over voltage category vii

index error 67
inlet module P
cleaning 58 physical dimensions 6
description 4 piston chambers 4
installation piston guide bushings, flushing 30, 57
checklist 10 piston seals, accessing 35
serial communication cable 12
pistons, cleaning 56
unpacking 10
platform, solvent 16
instrument casing, cleaning 56
Pollution Category 2 vii
Intercommunications Cable RS232 95
position error 67
power cable, connecting 11
K pressure sensor error 67
Keller LEO 1 Reference Manometer 71 Pressure Stability, testing 73
primary pistion seals, replacing 51
L proportioning accuracy, testing 64
proportioning valves 4
LDA 4
pulsation, tesing 63
accessing components 35
assembling 42 pump
dismantling 40 connecting 31
installing 46 environmental requirements 11
removing 35 powering on 32
seal wash (figure) 27
LDA body 4
Troubleshooting Guide 68
LED, status 5
purge valve 4
liquid displacement assembly (LDA) 4
Liquid Flowmeter 72
low pressure error 67 Q
quaternary pump, functional description 2
M
maintenance schedule 34 R
modular pump housing 2 replacement parts
motor error 67 inlet valve assembly 94
LDA 92
main body 91
O residual pulsation 63
operating specifications, degasser 7 RS232 Input/Output Cable 72
operating specifications, pump 7
outlet module, accessing 35

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S V
safety information vi vacuum degasser
seal wash pump 5 description 2
figure 27 vacuum degasser, description 4
installing 26 valve, purge 4
secondary piston seals, replacing 53
shipping container, contents 10 W
solenoid error 67
Witch with Bowden Cable Accela 600 97
solvent
platform 16
reservoir filter 16
solvent lines
connecting 15
solvent requirements ix
solvent reservoir bottles, assembling 15
specifications
degasser operating 7
electronic 7
physical dimensions 6
pump operating 6
system housing 2

T
Thermo Rheos Series Pump Force Sensor Calibration wizard
74
Thermo Rheos Series Transfer Volume Calibration Wizard
79
Tools, calibration 71
transfer volume calibration wizard 79
troubleshooting guide
degasser 69
pump 68

U
unpacking your pump 10
USB cable 12, 13
USB port 13

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114 600 Pump Service Manual Thermo Scientific

 Index: W

Thermo Scientific 600 Pump Service Manual 115

Index: W

116 600 Pump Service Manual Thermo Scientific

 Index: W

Thermo Scientific 600 Pump Service Manual 117

Index: W

118 600 Pump Service Manual Thermo Scientific