SCHOOL OF BIO SCIENCES AND

TECHNOLOGY

[Link]. Biotechnology

Curriculum

(2018-2019 admitted students)

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VISION STATEMENT OF VELLORE INSTITUTE OF TECHNOLOGY

Transforming life through excellence in education and research.

MISSION STATEMENT OF VELLORE INSTITUTE OF

TECHNOLOGY

World class Education: Excellence in education, grounded in ethics and

critical thinking, for improvement of life.
Cutting edge Research: An innovation ecosystem to extend knowledge and
solve critical problems.
Impactful People: Happy, accountable, caring and effective workforce and
students.
Rewarding Co-creations: Active collaboration with national & international
industries & universities for productivity and economic development.
Service to Society: Service to the region and world through knowledge and
compassion.
VISION STATEMENT OF THE SCHOOL OF BIO SCIENCES AND TECHNOLOGY
To nurture high-quality bioengineers and science graduates with the potential to
innovate, invent and disseminate knowledge for the benefit of society and
environment.
MISSION STATEMENT OF THE SCHOOL OF BIO SCIENCES AND
TECHNOLOGY

 To create opportunities for multi-disciplinary education, training and

research in biotechnology and bio-sciences.
 To instill a spirit of innovation and creativity in young minds from across
the globe with sound research aptitude.
 To foster ethically strong biologists who effectively contribute towards
the growth of the nation.

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 [Link]. BIOTECHNOLOGY

PROGRAMME EDUCATIONAL OBJECTIVES (PEOs)

1. Graduates will be practitioners and leaders in their chosen field

2. Graduates will function in their profession with social awareness
and responsibility
3. Graduates will interact with their peers in other disciplines in
their work place and society and contribute to the economic
growth of the country
4. Graduates will be successful in pursuing higher studies in their
chosen field
5. Graduates will pursue career paths in teaching or research

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 [Link]. BIOTECHNOLOGY

PROGRAMME OUTCOMES (POs)

PO_02: Having a clear understanding of the subject related concepts and of

contemporary issues and apply them to identify, formulate and analyse complex
engineering problems

PO_06: Having problem solving ability- to assess social issues (societal, health,
safety, legal and cultural) and engineering problems

PO_08: Having a clear understanding of professional and ethical responsibility

PO_09: Having cross cultural competency exhibited by working as a member or

in teams

PO_10: Having a good working knowledge of communicating in English –

communication with engineering community and society

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 [Link]. BIOTECHNOLOGY

ADDITIONAL PROGRAMME OUTCOMES (APOs)

APO_01: Having an ability to be socially intelligent with good SIQ (Social

Intelligence Quotient) and EQ (Emotional Quotient)

APO_04: Having computational thinking (Ability to translate vast data in to

abstract concepts and to understand database reasoning

APO_05: Having Virtual Collaborating ability

APO_07: Having critical thinking and innovative skills

APO_08: Having a good digital footprint

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 [Link]. BIOTECHNOLOGY

PROGRAMME SPECIFIC OUTCOMES (PSOs)

On completion of [Link]. (Biotechnology) programme,

graduates will be able to

 PSO1: Apply the principles of molecular biology methods with

emphasis on the application of recombinant DNA technology to
animals, plants and microbial organisms

 PSO2: Manipulate living organisms and biological systems to

produce products that advance healthcare, medicine, agriculture,
food, pharmaceuticals and environmental control

 PSO3: Ability to independently carry out research and

development work to solve the practical problems

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 [Link]. BIOTECHNOLOGY

CREDIT STRUCTURE

Category-wise Credit distribution

Category Credits
University core (UC) 29
Programme core (PC) 23
Programme elective 22
(PE)
University elective (UE) 6
Bridge course (BC) 0
Total credits 80

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 [Link]. BIOTECHNOLOGY
DETAILED CURRICULUM
University Core
S. No. Course Course Title L T P J C
Code
1 BST6099 Masters Thesis 0 0 0 0 14
2 MSM5001 Biostatistics 2 0 2 0 3
3 RES5001 Research Methodology 2 0 0 0 2
4 ENG5003 English for Science and Technology - LO 0 0 4 0 2
5 FRE5001 Francais fonctionnel-TH 2 0 0 0 2
6 GER5001 Deutsch fuer Anfaenger - TH 2 0 0 0 2
7 STS4001 Essentials of Business Etiquette and problem 3 0 0 0 1
solving
8 STS4002 Preparing for Industry 3 0 0 0 1
9 SET5001 Science, Engineering and Technology 0 0 0 0 2
Project - I
10 SET5002 Science, Engineering and Technology 0 0 0 0 2
Project - II
11 SET5003 Science, Engineering and Technology 0 0 0 0 2
Project - III

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 [Link]. BIOTECHNOLOGY
Programme Core

S. No. Course Course Title L T P J C

Code
1 BST5001 Microbiology 2 0 2 4 4
2 BST5002 Advanced Biochemistry 3 0 2 0 4
3 BST5003 Cell and Molecular Biology 3 0 0 0 3
4 BST5004 Immunology 2 0 0 0 2
5 BST5009 Analytical Techniques in Biotechnology 2 0 2 4 4
6 BST5010 Genetic Engineering 2 0 0 4 3
7 BST5011 Bioinformatics 2 0 0 4 3

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 [Link]. BIOTECHNOLOGY
Programme Electives

S. No. Course Course Title L T P J C

Code
1 BST5005 Medical Diagnostics 3 0 0 0 3
2 BST5006 Tissue Engineering and Regenerative 3 0 0 0 3
Medicine
3 BST5007 Medical Biotechnology 3 0 0 0 3
4 BST5008 Industrial Biotechnology 2 0 2 0 3
5 BST6001 Cancer Biology and Therapeutics 2 0 0 4 3
6 BST6002 Stem Cell Biology 3 0 0 0 3
7 BST6003 Clinical and Translational Research 3 0 0 0 3
8 BST6004 Forensic Science and Technology 2 0 0 4 3
9 BST6005 Pharmacology and Toxicology 3 0 0 0 3
10 BST6006 Medical Informatics 2 0 0 4 3
11 BST6007 Nutraceuticals 2 0 2 4 4
12 BST6008 Marine Biotechnology 3 0 0 0 3
13 BST6009 Nanobiotechnology 2 0 0 4 3
14 BST6010 Applied Enzyme Technology 3 0 2 0 4
15 BST6011 Metabolic Engineering 3 0 0 0 3
16 BST6012 Plant Biotechnology 2 0 2 4 4
17 BST6013 Bioremediation 2 0 0 4 3
18 BST6014 Genomics and Proteomics 3 0 0 0 3
19 BST6015 Signal Transduction 2 0 0 0 2
20 BST6016 Cellular and Molecular Biophysics 3 0 0 4 4

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University Electives (12 credits)

Course Pre
[Link] Course Title L T P J C [Link]
Code requisite
Any course offered to
1 [Link] ( Subject to CGPA 0 0 0 0 0 6
Conditions)/[Link]
Programs.

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 UNIVERSITY CORE

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 BST6099 Masters Thesis
Pre-requisite As per the academic regulations Syllabus version
1.0
Course Objectives:
To provide sufficient hands-on learning experience related to the area of specialization with a
focus on research orientation

Expected Course Outcome:

At the end of the course the student will be able to
1. Formulate specific problem statements for ill-defined real life problems with reasonable
assumptions and constraints.
2. Perform literature search and / or patent search in the area of interest.
3. Design and Conduct experiments
4. Perform error analysis / benchmarking / costing
5. Synthesise the results and arrive at scientific conclusions
6. Document the results in the form of technical report / presentation
Student Learning Outcomes (SLO): 9, 20
9. Having problem solving ability- solving social issues and engineering problems
20. Having a good digital footprint
Contents
[Link] be a theoretical analysis, modeling & simulation, experimentation & analysis, prototype
design, correlation and analysis of data, software development, applied research and any other
related activities.
2. Project can be for one or two semesters based on the completion of required number of
credits as per the academic regulations.
3. Should be individual work.
4. Carried out inside or outside the university, in any relevant industry or research institution.
5. Publications in the peer reviewed journals / International Conferences will be an added
advantage

Mode of Evaluation: Periodic reviews, Presentation, Final oral viva, Poster submission
Recommended by Board of Studies 04.03.2016
Approved by Academic Council 40th AC Date 18.03.2016

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Course code Course title L T P J C
MSM5001 Biostatistics 2 0 2 0 3
Pre-requisite NIL Syllabus version
v. 1.1
Course Objectives:
1. Understand the concepts behind collection and presentation of data
2. Understand the measures of central tendency and dispersion along with related analysis
3. Interpret and analyse data using statistical tools and design experiment

Expected Course Outcome:

1. Construct tables and graphs for data presentation
2. Explain measures of central tendency and dispersion along with predicting probability features
of experiments
3. Discuss the correlation between different types of data along with related variables.
4. Test hypothesis and carry out related statistical tests including that of significance.
5. Formulate designs for experiments.
6. Analyse and interpret practically, the data acquired in biological experiments, by using
statistical methods

Student Learning Outcomes (SLO): 1, 2, 10,18

1. Having an ability to apply mathematics and science in engineering applications.
2. Having a clear understanding of the subject related concepts and of contemporary issues.
[Link] a clear understanding of professional and ethical responsibility
[Link] critical thinking and innovative skills
Module:1 Descriptive methods 5 hours
Frequency Distribution, Characteristics of a Frequency Distribution, Tabular and Graphical
Presentation of Data: Line Graphs, Bar Charts, Histograms

Module:2 Measures of central tendency 5 hours

Arithmetic Mean, Median, Mode, Selection of the Appropriate Measure of Central Tendency,
Geometric Mean, Harmonic Mean

Module:3 Measures of dispersion 3 hours

Range, quartile Deviation, Mean Deviation, Variance and Standard Deviation, Skewness and
Kurtosis.

Module:4 Probability 3 hours

Probability Definition, Rules for Calculating Probabilities, Normal Distribution

Module:5 Correlation and Regression

Correlation , Karl Pearson correlation, Rank correlation, regression analysis

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Module:6 Data analysis and interpretation 5 hours
Tests of hypothesis, Tests of significance, st udent ’ s t- test, Non-parametric test: chi-square
test, Goodness of fit, Analysis of variance. F-test

Module:7 Experimental Design 3 hours

Designed Experiments Principles of experimental design, Blocking and Extraneous
Variables. Completely Randomized Design, Randomized block design

Module:8 Contemporary issues: 3 hours

Industrial Expert lecture

Total Lecture hours: 30 hours

Text Book(s)
1. Wayne W. Daniel, Chad L. Cross,2012, 10th edition, Biostatistics: A Foundation for
Analysis in the Health Sciences, Wiley Sciences Publisher
Reference Books
1. Gupta S.P., 2010, 5th Edition, Statistical Methods, Sultan chand & Sons.

Mode of Evaluation: CAT / Assignment / Quiz / FAT / Project / Seminar

List of Challenging Experiments (Indicative)

Determination of frequency distribution for raw data for population 2 Hours

1.

Estimation of mean ,median and mode 2 Hours

2.

Estimation of SD 2 Hours
3.

Estimation of variability 2 Hours

4.

Estimation of correlation coefficient 2 Hours

5.

Estimation of regression analysis 2 Hours

6.

Hypotesis testing 2 Hours

7.

Chi-square test 2 Hours

8.
9. 2 Hours
Challenging research problems on t test
10. 2 Hours
Challenging research problems on Estimation of one way ANOVA
11. 2 Hours
Challenging research problems on Estimation of two way ANOVA

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12. 2 Hours
Challenging research problems on Designs of experiments
13. 3 Hours
Challenging research problems on Determination of frequency distribution
for raw data for population

Challenging research problems on. Estimation of mean ,median and mode 3 Hours
14.
Total Laboratory Hours 30 hours
Mode of assessment:
Recommended by Board of Studies 03-08-2017
Approved by Academic Council No. 46 Date 24-08-2017

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Course code Course title L T P J C
RES5001 Research Methodology 2 0 0 0 2
Pre-requisite Syllabus version
v. 1.0
Course Objectives:
1. Impart skills to develop a research topic and design
2. Define a purpose statement, a research question or hypothesis, and a research objective
3. Analyze the data and arrive at a valid conclusion
4. Compile and present research findings

Expected Course Outcome:

1. Explain the basic aspects of research and its ethics
2. Outline research problems, their types and objectives
3. Formulate good research designs and carry out statistically relevant sampling
4. Collect, collate, analyze and interpret data systematically.
5. Experiment with animals ethically
6. Make use of literature and other search engines judiciously for research purposes

Student Learning Outcomes (SLO): 2,8

2 Having a clear understanding of the subject related concepts and of contemporary issues
8 Having Virtual Collaborating ability
Module:1 Introduction and Foundation of Research 2 hours
Meaning, Objectives, Motivation, Utility for research. Concept of theory, empiricism, deductive
and inductive theory. Characteristics of scientific method –Understanding the language of
research.

Module:2 Problem identification and formulation 4 hours

Scientific Research: Problem, Definition, Objectives, Types, Purposes and components of
Research problem

Module:3 Research Design 4 hours

Concept and Importance in Research : Features of a good research design, Exploratory
Research Design and Descriptive Research Designs

Module:4 Sampling 6 hours

Sampling methods, Merits and Demerits. Observation methods, Sampling Errors (Type I and
Type II). Determining size of the sample. Experimental Design: Concept of Independent
&Dependent variables.

Module:5 Data analysis and Reporting 6 hours

Fundamentals of Statistical Analysis and Inference, Multivariate methods, Concepts of
Correlation and Regression; Research Reports: Structure, Components, Types and Layout of
Research report and articles, Writing and interpreting research results, Figures and Graphs

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Module:6 Animal handling 2 hours
Guidelines-animal ethical committee, animal models, various routes of drug administrations,
LD50, ED50

Module:7 Use of encyclopedias and tools in research 4 hours

Research Guides, Handbook, Academic Databases for Biological Science Discipline. Methods to
search required information effectively.

Module:8 Contemporary issues: 2 hours

Total Lecture hours: 30 hours

Text Book(s)
1. Catherine Dawson, Introduction to research methods : a practical guide for anyone
undertaking a research project, Oxford : How To Books, Reprint 2010
2. Julius S. Bendat, Allan G. Piersol, Random Data: Analysis and Measurement Procedures,
4th
Edition, ISBN: 978-1-118-21082-6, 640 pages, September 2011
3. Research in Medical and Biological Sciences, 1st Edition, From Planning and Preparation to
Grant Application and Publication, Editos: Petter Laake Haakon Benestad Bjorn Olsen,
ISBN: 9780128001547, Academic Press, March 2015
Reference Books
1. John Creswell, Research Design: Qualitative, Quantitative, and Mixed Methods
Approaches, Fourth Edition (March 14, 2013)

Mode of Evaluation: CAT / Assignment / Quiz / FAT / Project / Seminar

Recommended by Board of Studies 03.08.2017
Approved by Academic Council No. 46 Date 24-08-2017

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Course code Course Title L T P J C
ENG5003 English for Science and Technology 0 0 4 0 2
(for MCA & [Link]., programmes)
Pre-requisite Cleared EPT Syllabus version
v. 1.1
Course Objectives:
1. To enable students communicate effectively in social, academic and professional contexts thereby
enhancing their interpersonal, managerial, problem-solving, and presentation skills.
2. To facilitate students develop their listening competency and critically evaluate and review
documentaries, talks and speeches.
3. To Assist students read and comprehend News Articles and Scientific Texts; effectively interpret
tables and graphs; write and proof-read official correspondences.
Expected Course Outcome:
1. Make effective presentations and display their interpersonal skills in academic and professional
contexts.
2. Emerge as good listeners and critically evaluate oral communication.
3. Excel in reading, comprehending and interpreting technical reports, texts and data.
4. Able to write effectively in English and also display their proof-reading abilities.
5. Face real interviews and handle personal and professional conflicts effectively.
Student Learning Outcomes (SLO): 16, 18, 20
16. Good working knowledge of communicating in English.
18. Critical thinking and innovative skills.
20. Having a good digital footprint.
Module:1 Career Goals 4 hours
Short term and long term career goals
Activity: SWOT Analysis/ Comprehending speeches
Module:2 Interpersonal Skills 4 hours
Interpersonal Communication in/with Groups (Corporate Etiquette: Journey from Campus to
corporate)
Activity: Role Plays/Mime/Skit
Module:3 Listening Skills 4 hours
Listening to Documentary
Activity: Critically evaluate/Review a documentary/TED Talk
Module:4 Reading Skills 4 hours
Skimming, Scanning, Intensive & Extensive reading
Activity: Reading News Papers/Magazines/Scientific Texts
Module:5 Report Writing 4 hours
Language and mechanics of writing report
Activity: Writing a Report/Mini Project
Module:6 Study Skills 4 hours
Summarizing the report
Activity: Abstract, Executive Summary, Digital Synopsis
Module:7 Interpreting skills 4 hours

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Interpret data in tables and graphs Activity: Transcoding
Module:8 Editing Skills 4 hours
Proof Reading
Sequencing
Activity: Editing any given text
Module:9 Presentation Skills 4 hours
Oral Presentation using digital tools
Activity: Oral presentation on the given topic using appropriate non-verbal cues
Module:10 Group Discussion 4 hours
Intragroup interaction (avoid, accommodate, compete, compromise, collaborate)
Activity: Group discussion on a given topic
Module:11 Professional Skills 4 hours
Résumé Writing
Activity: Prepare an Electronic Résumé
Module:12 Skill-Gap Analysis 4 hours
Tailor your skills to suit the Job needs
Activity: Write a SoP for higher Studies/Purpose Statement for job
Module:13 Interview Skills 4 hours
Placement/Job Interview
Activity: Mock Interview
Module:14 Managerial Skills 4 hours
Official Meeting to organize events
Activity: Writing Agenda, Minutes of Meeting (video conferencing) and Organizing an event
Module:15 Problem Solving Skills 4 hours
Conflict Management & Decision Making
Activity: Case analysis of a challenging Scenario
Total Lecture hours 60 hours

Text Book(s)
1. Kuhnke, E. Communication Essentials For Dummies. (2015). First Edition. John Wiley &
Sons.
2. Hewings, M. Advanced Grammar in Use Book with Answers and CD-ROM: A Self-Study
Reference and Practice Book for Advanced Learners of English. (2013). Third Edition.
Cambridge University Press. UK.
Reference Books
1. Churches, R. Effective Classroom Communication Pocketbook. Management Pocketbooks.
(2015). First Edition. USA.
2. Wallwork, A. English for Writing Research Papers. (2016). Second Edition. Springer.
3. Wood, J. T. Communication in Our Lives. (2016). Cengage Learning. Boston. USA.
4. Anderson, C. TED Talks: The Official TED Guide to Public Speaking. (2016). First
[Link]. Houghton Mifflin. New. York.
5. Zinsser, William. On writing well. HarperCollins Publishers. 2016. Thirtieth Edition. New
York.
6. Tebeaux, Elizabeth, and Sam Dragga. The essentials of Technical Communication. 2015. First
Edition Oxford University Press. USA.

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Mode of Evaluation: Mini Project, Flipped Class Room, Lecture, PPT’s, Role play, Assignments
Class/Virtual Presentations, Report and beyond the classroom activities
List of Challenging Experiments (Indicative)
1. Setting short term and long term goals 2 hours
2. Mime/Skit/ Activities through VIT Community Radio 6 hours
3. Critically evaluate / review a documentary/ Activities through VIT Community 4 hours
Radio
4. Mini Project 10 hours
5. Digital Synopsis 4 hours
6. Case analysis of a challenging Scenario 4 hours
7. Intensive & Extensive reading of Scientific Texts 4 hours
8. Editing any given text 8 hours
9. Group discussion on a given topic / Activities through VIT Community Radio 8 hours
10. Prepare a video résumé along with your video introduction and then create a 10 hours
website (in Google Sites/Webly/Wix) showcasing skills and achievements.
Total Laboratory Hours 60 hours
Mode of evaluation: Mini Project, Flipped Class Room, Lecture, PPT’s, Role play, Assignments
Class/Virtual Presentations, Report and beyond the classroom activities
Recommended by Board of Studies 22-07-2017
Approved by Academic Council No. 47 Date 24.08.2017

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Course code Course title L T P J C
FRE5001 Franicais Fonctionnel 2 0 0 0 2
Pre-requisite Syllabus version
v.1
Course Objectives:
The course gives students the necessary background to:
1. Demonstrate competence in reading, writing, and speaking basic French, including knowledge
of vocabulary (related to profession, emotions, food, workplace, sports/hobbies, classroom and
family).
2. Achieve proficiency in French culture oriented view point.

Expected Course Outcome:

The students will be able to
1. Remember the daily life communicative situations via personal pronouns, emphatic pronouns,
salutations, negations, interrogations etc.
2. Create communicative skill effectively in French language via regular / irregular verbs.
3. Demonstrate comprehension of the spoken / written language in translating simple sentences.
4. Understand and demonstrate the comprehension of some particular new range of unseen written
materials.
5. Demonstrate a clear understanding of the French culture through the language studied.

Student Learning Outcomes (SLO): 2,11

2. Having a clear understanding of the subject related concepts and of contemporary issues11 -
11. Having interest in lifelong learning
Module:1 Saluer, Se présenter, Etablir des contacts 9 hours
Les Salutations, Les nombres (1-100), Les jours de la semaine, Les mois de l’année, Les Pronoms
Sujets, Les Pronoms Toniques, La conjugaison des verbes réguliers, La conjugaison des verbes
irréguliers- avoir / être / aller / venir / faire etc.

Module:2 Présenter quelqu‟un, Chercher un(e) 9 hours

correspondant(e), Demander des nouvelles
d‟une personne.

La conjugaison des verbes Pronominaux, La Négation,

L’interrogation avec ‘Est-ce que ou sans Est-ce que’.

Module:3 Situer un objet ou un lieu, Poser des questions 9 hours

L’article (défini/ indéfini), Les prépositions (à/en/au/aux/sur/dans/avec etc.), L’article contracté,
Les heures en français, La Nationalité du Pays, L’adjectif (La Couleur, l’adjectif possessif,
l’adjectif démonstratif/ l’adjectif interrogatif (quel/quelles/quelle/quelles), L’accord des adjectifs
avec le nom, L’interrogation avec Comment/ Combien / Où etc.,

Module:4 Faire des achats, Comprendre un texte court, 8 hours

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 Demander et indiquer le chemin.
La traduction simple :(français-anglais / anglais –français)

Module:5 Trouver les questions, Répondre aux 7 hours

questions générales en français.
L’article Partitif, Mettez les phrases aux pluriels, Faites une phrase avec les mots donnés,
Exprimez les phrases données au Masculin ou Féminin, Associez les phrases.

Module:6 Comment ecrire un passage 9 hours

Décrivez :
La Famille /La Maison, /L’université /Les Loisirs/ La Vie quotidienne etc.

Module:7 Comment ecrire un dialogue 7 hours

Dialogue:
a) Réserver un billet de train
b) Entre deux amis qui se rencontrent au café
c) Parmi les membres de la famille
d) Entre le client et le médecin

Module:8 Invited Talk: Native speakers 2 hours

Total Lecture hours: 30 hours

Text Book(s)
1. Echo-1, Méthode de français, J. Girardet, J. Pécheur, Publisher CLE International, Paris 2010.
2 Echo-1, Cahier d’exercices, J. Girardet, J. Pécheur, Publisher CLE International, Paris 2010.
Reference Books
1. CONNEXIONS 1, Méthode de français, Régine Mérieux, Yves Loiseau,Les Éditions Didier,
2004.

2 CONNEXIONS 1, Le cahier d’exercices, Régine Mérieux, Yves Loiseau, Les Éditions

Didier, 2004.

3 ALTER EGO 1, Méthode de français, Annie Berthet, Catherine Hugo, Véronique M.

Kizirian, Béatrix Sampsonis, Monique Waendendries , Hachette livre 2006.

Mode of Evaluation: CAT / Assignment / Quiz / FAT

Recommended by Board of Studies 26.02.2016
Approved by Academic Council No.41 Date 17-06-2016

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Course code Course title L T P J C

GER5001 Deutsch für Anfänger 2 0 0 0 2

Pre-requisite NIL Syllabus version

v.1
Course Objectives:
The course gives students the necessary background to:
[Link] students to read and communicate in German in their day to day life
[Link] industry-ready
[Link] them understand the usage of grammar in the German Language.

Expected Course Outcome:

The students will be able to
1. Create the basics of German language in their day to day life.
2. Understand the conjugation of different forms of regular/irregular verbs.
3. Understand the rule to identify the gender of the Nouns and apply articles appropriately.
4. Apply the German language skill in writing corresponding letters, E-Mails etc.
5. Create the talent of translating passages from English-German and vice versa and To frame
simple dialogues based on given situations.

Student Learning Outcomes (SLO): 2,11

2. Having a clear understanding of the subject related concepts and contemporary issues
11. Having an interest in lifelong learning
Module:1 3 hours
Einleitung, Begrüssungsformen, Landeskunde, Alphabet, Personalpronomen, Verb Konjugation,
Zahlen (1-100), W-fragen, Aussagesätze, Nomen – Singular und Plural
Lernziel:
Elementares Verständnis von Deutsch, Genus- Artikelwörter

Module:2 3 hours
Konjugation der Verben (regelmässig /unregelmässig) die Monate, die Wochentage, Hobbys,
Berufe, Jahreszeiten, Artikel, Zahlen (Hundert bis eine Million), Ja-/Nein- Frage, Imperativ mit
Sie
Lernziel :
Sätze schreiben, über Hobbys erzählen, über Berufe sprechen usw.

Module:3 4 hours
Possessivpronomen, Negation, Kasus- AkkusatitvundDativ (bestimmter, unbestimmterArtikel),
trennnbare verben, Modalverben, Adjektive, Uhrzeit, Präpositionen, Mahlzeiten, Lebensmittel,
Getränke
Lernziel :

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Sätze mit Modalverben, Verwendung von Artikel, über Länder und Sprachen sprechen, über eine
Wohnung beschreiben.

Module:4 6 hours
Übersetzungen : (Deutsch – Englisch / Englisch – Deutsch)
Lernziel :
Grammatik – Wortschatz - Übung

Module:5 5 hours
Leseverständnis,Mindmap machen,Korrespondenz- Briefe, Postkarten, E-Mail
Lernziel :
Wortschatzbildung und aktiver Sprach gebrauch

Module:6 . 3 hours
Aufsätze :
Meine Universität, Das Essen, mein Freund oder meine Freundin, meine Familie, ein Fest in
Deutschland usw

Module:7 4 hours
Dialoge:
e) Gespräche mit Familienmitgliedern, Am Bahnhof,
f) Gespräche beim Einkaufen ; in einem Supermarkt ; in einer Buchhandlung ;
g) in einem Hotel - an der Rezeption ;ein Termin beim Arzt.
Treffen im Cafe

Module:8 2 hours
Guest Lectures/Native Speakers / Feinheiten der deutschen Sprache, Basisinformation über die
deutschsprachigen Länder
Total Lecture hours: 30 hours

Text Book(s)
1. Studio d A1 Deutsch als Fremdsprache, Hermann Funk, Christina Kuhn, Silke
Demme : 2012
Reference Books
1 Netzwerk Deutsch als Fremdsprache A1, Stefanie Dengler, Paul Rusch, Helen Schmtiz, Tanja
Sieber, 2013
2 Lagune ,Hartmut Aufderstrasse, Jutta Müller, Thomas Storz, 2012.
3 Deutsche SprachlehrefürAUsländer, Heinz Griesbach, Dora Schulz, 2011
4 ThemenAktuell 1, HartmurtAufderstrasse, Heiko Bock, MechthildGerdes, Jutta Müller und
Helmut Müller, 2010

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 [Link]
[Link]
[Link]
[Link]
[Link]

Mode of Evaluation: CAT / Assignment / Quiz / FAT

Recommended by Board of Studies 04.03.2016
Approved by Academic Council 41 Date 17.06.2016

Course code Course title L T P J C

STS 4001 Essentials of Business Etiquette and problem solving 3 0 0 0 1
Pre-requisite None Syllabus version

Course Objectives:
[Link] develop the students’ logical thinking skills
2. To learn the strategies of solving quantitative ability problems
3. To enrich the verbal ability of the students
4. To enhance critical thinking and innovative skills

Expected Course Outcome:

1. Enabling students to use relevant aptitude and appropriate language to express themselves
[Link] communicate the message to the target audience clearly
3. The students will be able to be proficient in solving quantitative aptitude and verbal ability questions of
various examinations effortlessly

Student Learning Outcomes (SLO): 7, 9, 12

[Link] computational thinking (Ability to translate vast data in to abstract concepts and to
understand database reasoning)
9. Having problem solving ability- solving social issues and engineering problems
12. Having adaptive thinking and adaptability
Module:1 Business Etiquette: Social and Cultural 9 hours
Etiquette and Writing Company Blogs and
Internal Communications and Planning and
Writing press release and meeting notes

Value, Manners, Customs, Language, Tradition, Building a blog, Developing brand message, FAQs',
Assessing Competition, Open and objective Communication, Two way dialogue, Understanding the
audience, Identifying, Gathering Information, Analysis, Determining, selecting plan, Progress check, Types
of planning, Write a short, catchy headline, Get to the Point –summarize your subject in the first
paragraph., Body – Make it relevant to your audience,

Module:2 Study skills – Time management skills 3 hours

[Link]. Biotechnology Page 26

Prioritization, Procrastination, Scheduling, Multitasking, Monitoring, working under pressure and adhering
to deadlines

Module:3 Presentation skills – Preparing presentation 7 hours

and Organizing materials and Maintaining
and preparing visual aids and Dealing with
questions

10 Tips to prepare PowerPoint presentation, Outlining the content, Passing the Elevator Test, Blue sky
thinking, Introduction , body and conclusion, Use of Font, Use of Color, Strategic presentation, Importance
and types of visual aids, Animation to captivate your audience, Design of posters, Setting out the ground
rules, Dealing with interruptions, Staying in control of the questions, Handling difficult questions

Module:4 Quantitative Ability -L1 – Number properties 11 hours

and Averages and Progressions and
Percentages and Ratios

Number of factors, Factorials, Remainder Theorem, Unit digit position, Tens digit position, Averages,
Weighted Average, Arithmetic Progression, Geometric Progression, Harmonic Progression, Increase &
Decrease or successive increase, Types of ratios and proportions

Module:5 Reasoning Ability-L1 – Analytical Reasoning 8 hours

Data Arrangement (Linear and circular & Cross Variable Relationship), Blood Relations,
Ordering/ranking/grouping, Puzzle test, Selection Decision table

Module:6 Verbal Ability-L1 – Vocabulary Building 7 hours

Synonyms & Antonyms, One-word substitutes, Word Pairs, Spellings, Idioms, Sentence completion,
Analogies

Total Lecture hours: 45 hours

Reference Books
1. Kerry Patterson, Joseph Grenny, Ron McMillan, Al Switzler (2001) Crucial Conversations: Tools for
Talking When Stakes are High. Bangalore. McGraw‐Hill Contemporary

2. Dale Carnegie, (1936) How to Win Friends and Influence People. New York. Gallery Books
3. Scott Peck. M (1978) Road Less Travelled. New York City. M. Scott Peck.

[Link]. Biotechnology Page 27

4. FACE (2016) Aptipedia Aptitude Encyclopedia. Delhi. Wiley publications
5. ETHNUS (2013) Aptimithra. Bangalore. McGraw-Hill Education Pvt. Ltd.
Websites:
1. [Link]
2. [Link]
3. [Link]
4. [Link]
5. [Link]
Mode of Evaluation: FAT, Assignments, Projects, Case studies, Role plays,
3 Assessments with Term End FAT (Computer Based Test)

[Link]. Biotechnology Page 28

Course code Course title L T P J C
STS 4002 Preparing for Industry 3 0 0 0 1
Pre-requisite None Syllabus version
1
Course  To challenge students to explore their problem-solving skills
Objectives:  To develop essential skills to tackle advance quantitative and verbal
ability questions
 To have working knowledge of communicating in English

Expected Course  Enabling students to simplify, evaluate, analyze and use functions and
Outcome: expressions to simulate real situations to be industry ready.
 The students will be able to interact confidently and use decision
making models effectively
 The students will be able to be proficient in solving quantitative
aptitude and verbal ability questions of various examinations
effortlessly

Student Learning Outcomes (SLO): 9, 10

9. Having problem solving ability- solving social issues and engineering problems
10. Having a clear understanding of professional and ethical responsibility
Module:1 Interview skills – Types of interview 3 hours
and Techniques to face remote
interviews and Mock Interview

Structured and unstructured interview orientation, Closed questions and hypothetical questions,
Interviewers' perspective, Questions to ask/not ask during an interview, Video interview¸ Recorded
feedback, Phone interview preparation, Tips to customize preparation for personal interview,
Practice rounds

Module:2 Resume skills – Resume Template and 2 hours

Use of power verbs and Types of
resume and Customizing resume
Structure of a standard resume, Content, color, font, Introduction to Power verbs and Write up,
Quiz on types of resume, Frequent mistakes in customizing resume, Layout - Understanding
different company's requirement, Digitizing career portfolio

Module:3 Emotional Intelligence - L1 – 12 hours

Transactional Analysis and Brain
storming and Psychometric Analysis
and Rebus Puzzles/Problem Solving

Introduction, Contracting, ego states, Life positions, Individual Brainstorming, Group

Brainstorming, Stepladder Technique, Brain writing, Crawford's Slip writing approach, Reverse

[Link]. Biotechnology Page 29

brainstorming, Star bursting, Charlette procedure, Round robin brainstorming, Skill Test,
Personality Test, More than one answer, Unique ways

Module:4 Quantitative Ability-L3 – 14 hours

Permutation-Combinations and
Probability and Geometry and
mensuration and Trigonometry and
Logarithms and Functions and
Quadratic Equations and Set Theory

Counting, Grouping, Linear Arrangement, Circular Arrangements, Conditional Probability,

Independent and Dependent Events, Properties of Polygon, 2D & 3D Figures, Area & Volumes,
Heights and distances, Simple trigonometric functions, Introduction to logarithms, Basic rules of
logarithms, Introduction to functions, Basic rules of functions, Understanding Quadratic Equations,
Rules & probabilities of Quadratic Equations, Basic concepts of Venn Diagram

Module:5 Reasoning ability-L3 – Logical 7 hours

reasoning and Data Analysis and
Interpretation

Syllogisms, Binary logic, Sequential output tracing, Crypto arithmetic, Data Sufficiency, Data
interpretation-Advanced, Interpretation tables, pie charts & bar chats

Module:6 Verbal Ability-L3 – Comprehension 7 hours

and Logic

Reading comprehension, Para Jumbles, Critical Reasoning (a) Premise and Conclusion, (b)
Assumption & Inference, (c) Strengthening & Weakening an Argument

Total Lecture hours: 45 hours

References Michael Farra and JIST Editors(2011) Quick Resume & Cover Letter
Book: Write and Use an Effective Resume in Just One Day. Saint Paul,
Minnesota. Jist Works
 Daniel Flage Ph.D(2003) The Art of Questioning: An Introduction to
Critical Thinking. London. Pearson
 FACE(2016) Aptipedia Aptitude [Link]. Wiley
publications
Mode of Evaluation: FAT, Assignments, Projects, Case studies, Role plays,
3 Assessments with Term End FAT (Computer Based Test)

[Link]. Biotechnology Page 30

Course code SCIENCE, ENGINEERING AND TECHNOLOGY L T P J C
PROJECT– I
SET 5001 0 0 0 0 2
Pre-requisite Syllabus Version
Anti-requisite 1.10
Course Objectives:
 To provide opportunity to involve in research related to science / engineering
 To inculcate research culture
 To enhance the rational and innovative thinking capabilities

Expected Course Outcome:

On completion of this course, the student should be able to:
1. Identify problems that have relevance to societal / industrial needs
2. Exhibit independent thinking and analysis skills
3. Demonstrate the application of relevant science / engineering principles

SLO : 14, 18 & 20

Modalities / Requirements
1. Individual or group projects can be taken up
2. Involve in literature survey in the chosen field
3. Use Science/Engineering principles to solve identified issues
4. Adopt relevant and well-defined / innovative methodologies to fulfill the specified objective
5. Submission of scientific report in a specified format (after plagiarism check)

Student Assessment : Periodical reviews, oral/poster presentation

Recommended by Board of Studies 17-08-2017
Approved by Academic Council No. 47 Date 05-10-2017

[Link]. Biotechnology Page 31

Course code SCIENCE, ENGINEERING AND TECHNOLOGY L T P J C
PROJECT– II
SET 5002 0 0 0 0 2
Pre-requisite Syllabus Version
Anti-requisite 1.10
Course Objectives:
 To provide opportunity to involve in research related to science / engineering
 To inculcate research culture
 To enhance the rational and innovative thinking capabilities

Expected Course Outcome:

On completion of this course, the student should be able to:
4. Identify problems that have relevance to societal / industrial needs
5. Exhibit independent thinking and analysis skills
6. Demonstrate the application of relevant science / engineering principles

SLO : 14, 18 & 20

Modalities / Requirements
6. Individual or group projects can be taken up
7. Involve in literature survey in the chosen field
8. Use Science/Engineering principles to solve identified issues
9. Adopt relevant and well-defined / innovative methodologies to fulfill the specified objective
10. Submission of scientific report in a specified format (after plagiarism check)

Student Assessment : Periodical reviews, oral/poster presentation

Recommended by Board of Studies 17-08-2017
Approved by Academic Council No. 47 Date 05-10-2017

[Link]. Biotechnology Page 32

Course code SCIENCE, ENGINEERING AND TECHNOLOGY L T P J C
PROJECT– III
SET 5003 0 0 0 0 2
Pre-requisite Syllabus Version
Anti-requisite 1.10
Course Objectives:
 To provide opportunity to involve in research related to science / engineering
 To inculcate research culture
 To enhance the rational and innovative thinking capabilities

Expected Course Outcome:

On completion of this course, the student should be able to:
7. Identify problems that have relevance to societal / industrial needs
8. Exhibit independent thinking and analysis skills
9. Demonstrate the application of relevant science / engineering principles

SLO : 14, 18 & 20

Modalities / Requirements
11. Individual or group projects can be taken up
12. Involve in literature survey in the chosen field
13. Use Science/Engineering principles to solve identified issues
14. Adopt relevant and well-defined / innovative methodologies to fulfill the specified objective
15. Submission of scientific report in a specified format (after plagiarism check)

Student Assessment : Periodical reviews, oral/poster presentation

Recommended by Board of Studies 17-08-2017
Approved by Academic Council No. 47 Date 05-10-2017

[Link]. Biotechnology Page 33

 PROGRAMME CORE

[Link]. Biotechnology Page 34

 Course code Course title L T P J C
BST5001 Microbiology 2 0 2 4 4
Pre-requisite Nil Syllabus version
v. 1.1
Course Objectives:
1. Relate insight on the various aspects of Microbiology
2. Study the microbes present in various ecological niches
3. Inculcate the practical knowledge to the students in the field of microbiology

Expected Course Outcome:

1. Defining the evolution, physiological and growth aspects of microbes
2. Identifying the knowledge on the various medically important bacteria
3. Formulating the central dogma of viral infection and its types
4. Categorizing the knowledge in fungal infections
5. Interpreting the importance of various microbes from different ecological niches
6. Combining the studies on communication between bacteria and designing new methodologies in
quorum sensing and metagenomics
Student Learning Outcome (SLO): 2,11
2. Having a clear understanding of the subject related concepts and of contemporary issues
11. Having interest in lifelong learning
Module:1 Basics in microbiology: 4 hours
Classification of microbes - polyphasic taxonomy, microbial cell structures, microbial growth and
metabolism

Module:2 Bacteriology: 6 hours

Human microbiota, Epidemiology, pathogenesis, treatment and prophylaxsis – wound infections,
Upper and lower respiratory tract infections, Gastro intestinal infections and genito-urinary tract
infections, sexually transmitted diseases, antibiotic resistance.

Module:3 Virology: 3 hours

Medically important viruses – RNA and DNA viruses (Note: Two viruses in each type)

Module:4 Mycology 5 hours

Superficial, systemic and opportunistic, Vector borne diseases - Malarial parasite, Leishmaniosis
and Filariasis

Module:5 Microbes in Biome: 3 hours

Extremophiles and its types, application of microbes from various ecological niches –
Hydrothermal vent, unexplored forest soil and marine sediments

Module:6 Industrial microbiology: 4 hours

Industrially important microbes and its applications, Production of various fermented products –
Enzymes, cheese, wine and beer, probiotics and its bioactive compounds in human benefits

[Link]. Biotechnology Page 35

 Module:7 Recent developments in Microbiology: 3 hours
Biofilm and quorum sensing, Quorum quenching, Metagenomics for bioprospective approach –
Next-generation sequencing and pyrosequencing

Module:8 Contemporary issues: 2 hours

Total Lecture hours: 30 hours

Text Book(s)
1. Willey, J., 2014. Prescott, Harley and Klein's microbiology - 9th international ed./Joanne M.
willey, linda M. sherwood, christopher J. woolverton. New York: McGraw-Hill Higher
Reference Books
Education.
1. Ananthanarayan and JayaramPaniker (2013), “Text book of Medical Microbiology”, 9th
edition. Orient Longman publications.
2. Cappuccino, J.G. and Sherman, N., 2013. Microbiology: a laboratory manual (10th Edition).
Pearson/Benjamin Cummings.
3. Recent publications in the relevant field
List of Challenging Experiments (Indicative)
1. Isolation and purification of bacteria from various environments 2 hours
2. Isolation and purification of fungus from various ecological niches – Slide 2 hours
culture technique and hyphal tip method
3. Morphological characterization of the bacteria by staining and Non-staining 2 hours
Methods
4. Morphological characterization of the fungi by LPCB staining 2 hours
5. Biochemical characterization of bacteria by IMViC, catalase, oxidase, TSI, 2 hours
carbohydrate fermentation tests
6. Screening of the bacterial isolate for various traits like bioactive compound 2 hours
Production
7. Screening for pigment producing fungi 2 hours
8. Mass production of the effective bacteria and fungi 2 hours
9. Extraction and confirmation of the screened trait 2 hours
10. Detection of candidate gene responsible for the specific trait 2 hours

Total Laboratory Hours 20 hours

Mode of Evaluation: CAT/Assignments/Quiz/Project/FAT
Project „J‟ component
Recommended by Board of Studies 03-08-2017
Approved by Academic Council No.46 Date 24-08-2017

[Link]. Biotechnology Page 36

Course code Course title L T P J C
BST5002 Advanced Biochemistry 3 0 2 0 4
Pre-requisite Nil Syllabus version
v.1.1
Course Objectives:
1. Explain the basic molecular properties of biological molecules
2. Demonstrate how the structure of biological molecules dictates function
3. Illustrate how regulatory systems maintain homeostasis in biological system

Expected Course Outcome:

1. Relate the key principles of biochemistry at an advanced level
2. Translate the importance of biological macromolecules and their role in living systems
3. Summarize an advanced level of understanding about the structure and function relationship of
proteins
4. Interpret the correct folding of proteins for their right function
5. Compare the role of lipids in biological systems and movement of molecules across membranes
6. Illustrate how the enzymes catalyse reactions as well as the enzyme kinetics
7. Compile the different metabolic pathways, their interconnections and their regulation in living
organisms
Student Learning Outcomes (SLO): 2 ,11
2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Carbohydrates and Glycobiology:
5 hours
Classification of carbohydrates, glycosaminoglycans, proteoglycans, sialic acid, lectins,
carbohydrate arrays, glycans in health and disease, glycomimetics, Applications of
glycoconjugates.

Module 2 Protein Structure and Function: 6 hours

Classification of amino acids and titration curves; biologically important peptides; Proteins- levels
of organization; Ramachandran's plot; Structure and function of Mb, Hb and collagen.

Module:3 Protein Folding and Characterization: 5 hours

Protein folding- molecular chaperones, thermodynamics of folding and unfolding, models of
protein folding, misfolding diseases, protein purification and structure investigation, sequencing,
and solid phase Merrifield peptide synthesis.

Module:4 Membrane Transport and Nucleic acids: 6 hours

Classification of lipids; lipid bilayers, micelles, liposomes, membrane structure and assembly,
transport of molecules across membrane-channels and pumps, model membrane systems and their
applications; Structure of DNA and RNA, DNA reassociation kinetics, chromatin.

[Link]. Biotechnology Page 37

Module:5 Enzyme Kinetics: 5 hours
Properties of enzymes, classification, Michaelis Menten equation, kinetic parameters, Lineweaver
-Burk plot, factors affecting enzyme activity, enzyme inhibition, multisubstrate reactions, enzyme
units.

Module:6 Molecular Basis of Enzyme Catalysis and 5 hours

Regulation:
Different catalytic strategies, Mechanism of RNase, chymotrypsin, carbonic anhydrase and
lysozyme; Regulation of enzymes by- allosteric control, covalent modification, proteolytic
cleavage and isoenzymes.

Module:7 Regulation of Metabolic Pathways and 11 hours

Bioenergetics:
Metabolism; Glycolysis, TCA cycle, gluconeogenesis, glycogen metabolism & their regulation;
Fatty acid biosynthesis and oxidation; Biosynthesis of purine & pyrimidine nucleotides; Over
view of amino acid biosynthesis & urea cycle. Bioenergetics-Thermodynamic principles,
structure & function of individual complexes of electron transport chain (ETC) in mitochondria,
oxidative phosphorylation, shuttle systems, Inhibitors of ETC, uncouplers. Photosynthetic
electron transport & regulation.

Module:8 Contemporary issues: 2 hours

Industry expert lecture on recent advances in biochemistry and contemporary issues

Total Lecture hours: 45 hours

Text Book(s)
1. David Lee Nelson, Michael M. Cox. Lehninger Principles of Biochemistry. WH
Freeman;7th ed. (2017).
2 Jeremy M. Berg, John L. Tymoczko, LubertStryer. Biochemistry.7 th Edition, Palgrave
MacMillan (2011)
3 Donald Voet, Judith G. Voet. Biochemistry. 4th Edition, Wiley India Pvt Ltd (2011)
Reference Books
1. Christopher K. Mathews, Kensal E. van Holde, Dean R. Appling, Spencer J. Anthony-Cahill
Biochemistry. Prentice Hall; 4th Edition (2012).
2. Reginald H. Garrett, Charles M. Grisham. Biochemistry. Brooks Cole 5th Edition (2012).
th
David Bender, Kathleen M. Botham, Robert Murray. Harpers Illustrated Biochemistry. 29
Edition, McGraw-Hill Medical Publishing (2012).
3. Thomas M. Devlin, Textbook of Biochemistry with Clinical Correlations. 7th Edition, John
Wiley & Sons (2010).
4 Robert A. Horton, Principles of Biochemistry.5th Edition, Pearson Educacion (2011).

[Link]. Biotechnology Page 38

5 Richard A Harvey, Denise R Ferrier, Lippincott’s Illustrated Reviews Biochemistry. 5th
Edition, Lippincott Williams and Wilkins (2010).
6 Philip W. Kuchel and G.B. Ralston. Schaum's Outline of Biochemistry, Third Edition Int.
Ed., McGraw-Hill Book Co (2011).
th
7 Trudy McKee, James R. McKee Biochemistry: The Molecular Basis of Life, 6 Edition
Oxford University Press, (2015).

Mode of Evaluation: CAT / Assignment / Quiz/ FAT

Recommended by Board of Studies 03-08-2018
Approved by Academic Council No.46 Date 24-08-2017

[Link]. Biotechnology Page 39

 Course code Course title L T P J C
BST5003 Cell and Molecular Biology 3 0 0 0 3

Pre-requisite Nil Syllabus version

v. 1.1
Course Objectives:
1. This course will outline students to the dynamic relationships between cell structure and the
biochemical reactions that are necessary for cell growth, differentiation, survival and death with an
emphasis on eukaryotic cells
2. Familiarization of students with the experimental approaches used in molecular biology principles.
3. Inferring the concepts and mechanisms related to cell and molecular biology

Expected Course Outcome:

[Link] the Concepts of Cell and Molecular biology to apply in various research areas
[Link] hypotheses and select, adapt and conduct molecular and cell-based experiments to either
confirm or reject the hypotheses.
3. Attain a basic conceptual knowledge how gene expression is regulated at different levels, how
tissue-specific expression is achieved and exemplify how gene expression can be manipulated and
studied experimentally
[Link] and build the principles and techniques of molecular biology which prepares students for
further education and/or employment in teaching and basic research.
5. Inspect relevant information from research publications dealing with issues of cell and molecular
biology and assess and relate the information to the context of cell biology
6. Compile an appreciation for all levels of biological organization, including the molecular, cellular,
organismal, and systems levels.

Student Learning Outcomes (SLO): 2,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
11. Having interest in lifelong learning
Module:1 Introduction 6 hours
Overview of cellular organelle and their functions- comparison between plant and animal cells; 1
Cell wall; Plasma membrane; Modification of plasma membrane and intracellular junctions;
Protoplasm.
Mitochondria; Chloroplast; ER; Golgi complex; Lysosome, endosome; Ribosome; Centriole; Nucleus.
Module:2 Membrane transport 6 hours
Overview, membrane dynamics, ATP-powered pumps, non-gated ion channels, movement of water,
trans- epithelial transport, voltage-gated ion channels; Membrane trafficking: Endocytosis and
exocytosis.
Module:3 The cytoskeleton 6 hours
Dynamics of actin assembly, myosin-powered cell movements, cell locomotion, intermediate
filaments.
microtubule organization and dynamics, kinesin and dynein-powered movements.

[Link]. Biotechnology Page 40

 Module:4 Genes and Chromosomes 6 hours
Molecular definition of a gene, chromosomal organization of genes and noncoding DNA, mobile
DNA,functional rearrangements in chromosomal DNA, organizing cellular DNA into chromosomes,
organelle DNAs, the interrupted gene, genome evolution, telomeres, nucleosomes, transposition.

Module:5 DNA replication 6 hours

The chemistry of DNA synthesis, the mechanism of DNA polymerase, the replication fork,
initiation of DNA replication- helicase, termination of replication- topoisomerase, telomerase.

Module:6 Transcription and its regulation 7 hours

Prokaryotic and eukaryotic transcription-initiation, elongation and termination; regulation of
transcription; RNA splicing and processing-spliceosome, alternative splicing, RNA editing, mRNA
transport, catalytic RNA. Regulation of gene expression: epigenetic gene regulation, regulatory
RNAs- riboswitches, CRISPR,
miRNA, long non-coding RNAs, RNA interference.
Module:7 Translation and its regulation 6 hours
Messenger RNA, transfer RNA, ribosome; Initiation, elongation and termination; post-
translational modification in cellular functioning; regulation of translation.

Module:8 Contemporary issues: 2 hours

Industry experts lectures in the field of Cell and Molecular Biology

Total Lecture 45 hours

hours:
Text Book(s)
1. Molecular Biology of the Gene. Seventh Edition (2013). James D. Watson, Tania A. Baker,
Stephen
2. Molecular Cell Biology, Eighth Edition (2013). Harvey Lodish, Arnold Berk. W. H. Freeman
P. Bell. Pearson. Cold Spring Laboratory Press, NY, USA.
Co.,
Reference Books
1. USA.
Molecular Biology of the Cell. Sixth Edition (2014). Bruce Alberts, Alexander Johnson, Julian
Lewis,
Mode of Evaluation: CAT / Assignments / Quiz / FAT
et al. Garland Science. UK.
Recommended by Board of Studies 03-08-2017
Approved by Academic Council No.46 Date 24-08-2017

[Link]. Biotechnology Page 41

Course code Course title L T P J C
BST5004 Immunology 2 0 0 0 2
Pre-requisite NIL Syllabus version
v.1.1
Course Objectives:
1. Simplify the major components of the immune system.
[Link] the molecular and cellular basis of the development and function of the immune
system in states of health and disease.
3. Extend the potential applications and principles of translational and clinical research related to
the field of immunology
4. Design the skills necessary for the critical analysis of contemporary literature on topics related
to health and diseases.

Expected Course Outcome:

[Link] and discuss the role of the immune cells in health and disease.
[Link] the basic mechanisms that regulate immune responses and maintain tolerance
Summarize how the innate and adaptive immune system collaborate to fight infections
3. Translate the understanding of basic mechanisms into identification of biological, clinical and
therapeutic implications
4. Construct the knowledge of immunology into clinical decision-making through case studies.
5. Justify the experiments and techniques employed in relevant fields of immunological research
and disease diagnosis.
Student Learning Outcomes (SLO): 2,11
2. Having a clear understanding of the subject related concepts and of contemporary issues
11. Having interest in lifelong learning
Module:1 Introduction to the Immune System: 4 hours
Overview of the immune system, Innate and Adaptive immunity, Hematopoiesis, hematopoietic
growth factors and regulation. Cells and organs of the immune system. Stem cells and its clinical
uses.

Module 2 Molecular Immunology: 6 hours

Antigens, structure of antigen and its different types. Antibody structure and types. Antigen
processing and presentation, mechanism of antigen recognition

Module:3 Major Histocompatibility Complex: 3 hours

MHC organization – Class I, II and III and MHC restriction. Complement system, pathways.
Biological consequences and diseases, Cytokines
Module:4 Cellular immunology: 5 hours
Biology of T and B-lymphocytes, T helper cells, Cytotoxic T cells, Signal transduction

[Link]. Biotechnology Page 42

molecules associated with membrane immunoglobulin and T cells. Importance of co-stimulatory
molecules involved in B and T cell activation.
Module:5 Immunopathology: 3 hours
Autoimmunity and autoimmune disorders, Hypersensitivity reactions, transplantation and tumor
Immunology, immunotherapy for tumors and autoimmune disorders, immunodeficiency diseases.

Module:6 Therapeutic Immunology: 4 hours

Vaccines, active and passive immunization, DNA and plant based vaccines, AIDS vaccine,
Recombinant antigen as vaccine, Monoclonal antibodies and their use in diagnosis.

Module:7 Immunotechnology: 3 hours

ELISA, Immunoelectrophoresis, Immuno-blotting, Immunohistochemistry and
Immunofluorescence

Module:8 Contemporary Issues: 2 hours

Industry expert lectures on contemporary issues

Total Lecture hours: 30 hours

Text Book(s)
1. Janis Kuby (2013), Immunology, 7th edition. By Owen, Punt and Stranford Textbook. W.H
freeman and company.
Reference Books
1. Chapel H, Haeney M, Misbah S and Snowden N,(2014) Essentials of Clinical Immunology
6th Edition, Wiley Blackwell
Kenneth Murphy and Casey Weaver (2016), Janeway’ s Immunobiology – The Immune
2. system in Health and disease, 9th edition, Garland Science Publishing (Taylor and Francis
Group).
Abbas AK, Lichtman AH, Pillai S (2011) Cellular and molecular immunology, 8th edition,
3. Elsevier Health Sciences

Mode of Evaluation: Assignments, Seminars, Continuous assessment tests and Final

assessment test.

Recommended by Board of Studies 03-08-2017

Approved by Academic Council No.46 Date 24-08-2017

[Link]. Biotechnology Page 43

Course code Course title L T P J C
BST5009 Analytical Techniques in Biotechnology 2 0 2 4 4

Pre-requisite Nil Syllabus version

v.1.0
Course Objectives:
1. Interpret the various analytical techniques used in life sciences
2. Compare the application of various analytical techniques in biotechnology and related fields
[Link] the scientific understanding of various analytical techniques and interpretation of
results

Expected Course Outcome:

1. Relating the key concepts of buffers, HH equation and its applications
2. Outlining the importance of chromatography methods for separation and purification of analytes
and establish an advanced level of understanding about HPLC and GC instruments and their
applications in research and development
3. Elaborating the importance of electrophoretic procedures for the separation of biological
molecules mainly proteins and nucleic acids
4. Examining the importance of centrifugal separation methods in life sciences.
5. Justify the importance of electron and light microscopic techniques for the investigation of
finer details of both cellular and non cellular structures
6. Classifying different spectroscopic techniques to understand and elucidate the structure,
mechanism of biological molecules

Student Learning Outcomes (SLO): 2 ,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
11. Having interest in lifelong learning
Module:1 Electrochemical Techniques: 3 hours
Acid base theories, pH, Buffers and preparation, Henderson-Hasselbach equation, pH meter,
standard hydrogen electrode.

Module:2 Chromatography Techniques-I: 4 hours

Classification of chromatographic methods, Principle, methodology and applications of paper, thin
layer, gel permeation, ion exchange and affinity chromatography. Performance parameters.

Module:3 Chromatography Techniques –II: 3 hours

Principle, Instrumentation and applications of High Performance Liquid Chromatography (HPLC)
and Gas Chromatography (GC). Different types of columns and detectors used in HPLC and GC.

Module:4 Electrophoretic Techniques: 4 hours

Electrophoresis-basic principles, Principle, procedure and applications of paper, cellulose acetate,
polyacrylamide and agarose gel electrophoresis, electro-blotting, isoelectric focusing; 2D PAGE,
immuno electrophoresis, capillary electrophoresis and Pulsed Field Gel electrophoresis (PFGE).

Module:5 Sedimentation and Centrifugation 4 hours

[Link]. Biotechnology Page 44

 Techniques:
Basic principles of sedimentation, types of rotors, types of centrifuges, Types of centrifugal
separation- differential centrifugation, density gradient centrifugation; Principle, instrumentation
and applications of preparative and analytical ultracentrifuge.

Module:6 Microscopy: 5 hours

Properties of light, important concepts in microscopy, Basic principle, instrumentation and
applications of - Bright field, Dark field, Phase contrast, Fluorescence and Confocal, Principle,
instrumentation and application of- Transmission electron microscope, Scanning electron
microscope, Atomic force microscope.

Module:7 Spectroscopy: 5 hours

Electromagnetic radiation, Transitions in spectroscopy, UV-Visible spectroscopy- electronic
transitions, absorption laws, Instrumentation of Single, double beam spectrophotometers;
Spectrofluorimetry, chromophores, auxochrome, absorption bands, Applications. Principle,
instrumentation and application of –Flame Spectrophotometer, Atomic Absorption Spectrometer,
Infra-red spectrophotometer. Over view of – NMR, ESR and MS.

Module:8 Contemporary issues: 2 hours

Total Lecture hours: 30 hours

Text Book(s)
1. Principles and Techniques of Biochemistry and Molecular Biology (2010) 7th Edition by
Keith Wilson.
2. Physical Biochemistry: Principles and Applicationsby David Sheehan 2 nd Edition, John
Wiley & Sons (2009)
Reference Books
1. Biochemistry Laboratory: Modern Theory and Techniques, Rodney F. Boyer, Pearson
Prentice Hall; 2nd Edition (2010).
2. Bioanalytical Techniques, Sekhar Talluri, I.K. Internationlal Publishing House Pvt. Ltd.
(2012)
3. Biochemical methods of analysis: Theory and applications. Saroj Dua and Neera Garg, Alpha
Science Intl Ltd; 1 st Edition (2010)
List of Challenging Experiments (Indicative)
1. Preparation of a buffer using HH equation and determination of buffer 2 hours
capacity.
2. Separation and identification of plant pigments by Thin Layer 2 hours
Chromatography (TLC).
3. Separation of Plant Pigments by silica column chromatography (using silica 3 hours
gel-G).

[Link]. Biotechnology Page 45

4. Separation of proteins by gel filtration chromatography (GFC). 3 hours

5. Purification of lysozyme from egg white by ion exchange chromatography 4 hours

(IEC).
6. Separation of proteins by SDS polyacrylamide gel electrophoresis (SDS- 2 hours
PAGE).

7. Isolation of Genomic DNA and analysis by Agarose gel electrophoresis. 4 hours

8. Verification of Beer - Lambert’s law by UV-Vis spectrophotometer. 2 hours

9. Determination of sodium by flame photometry. 2 hours

10. Isolation of plasmid DNA. 4 hours

11. Demonstration- HPLC, GC, SEM, TEM, AAS, FTIR. 2 hours

Total Laboratory Hours 30 hours

Mode of Evaluation: CAT / Assignments / Quiz / FAT
Recommended by Board of Studies 03-08-2017
Approved by Academic Council No. 46 Date 24-08-2017

[Link]. Biotechnology Page 46

 Course code Course title L T P J C
BST5010 Genetic Engineering 2 0 0 4 3
Pre-requisite Syllabus version
v. 1.1
Course Objectives:
1. Outlining the methods and techniques involved in manipulation of DNA, RNA and Proteins.
2. Maximize the enthusiasm to know recent developments in the subject.
3. Make students interpret the ethical and environmental problems associated with genetic
engineering.

Expected Course Outcome:

1. Translate the key concepts, facts, and theories relevant to gene modification.
2. Create enthusiasm to know the recent developments in the subject.
3. Illustrate the contemporary issues in related field.
4. Build consciousness about the environmental health.
5. Justify the relevance of scientific hypothesis and scientific methodologies.
6. Able to discover scientific knowledge for betterment of life.
2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Student Learning Outcomes (SLO): 2,11
2. Having a clear understanding of the subject related concepts and of contemporary issues
11. Having interest in lifelong learning
Module:1 Introduction to genetic engineering 2 hours
Importance and outline of recombinant DNA technology, organization of genes in the
genome, gene expression.

Module:2 Enzymes in genetic engineering 4 hours

Endo- and exonucleases, DNase, RNase; Restriction endonucleases- types, and mechanism of
action;restriction modification- methylases; other enzymes- methyl transferases, phosphatases,
polynucleotide kinase, polynucleotide phosphorylase; Ligases - types and mechanism of
action, linker, adaptor,homopolymer tailing, ligation with DNA topoisomerase; Polymerases-
types and mechanism of action;reverse transcriptase.

Module:3 Vectors in genetic engineering 4 hours

Role of promoters and terminators; plasmids- types of plasmids; Bacteriophages- lytic,
lysogenic; M13 phage, cosmids. cloning vectors- pBR322, pUC8, pGEM3Z; insertion- and
replacement vectors; phage P1vector system; artificial chromosomes- BAC, YAC; Cloning
vectors for higher plants- Agrobacterium;plant virus based vectors; cloning vectors for animals,
viruses as cloning vectors for mammals.

Module:4 Cloning strategies 4 hours

[Link]. Biotechnology Page 47

 Cloning cDNA in plasmid vectors, cloning cDNA in bacteriophage vectors, and construction
of cDNA
library. Construction of genomic libraries. Advanced cloning strategies: multigene cloning and
gateway cloning, Ligation independent cloning (LIC), Gibson DNA assembly, Circular
polymerase extension cloning. Bioinformatic tools and molecular cloning softwares for gene
design and cloning.
Module:5 Gene expression analysis; Issues in transgene expression.
Transformation 3 hours
Preparation of competent cells of bacteria; selection for transformed cells physical, chemical
and biological methods of gene transfer: physical- microinjection, electroporation, biolistic,
ultrasound; chemical- calcium phosphate precipitation method, PEI, dendrimers; biological-
liposome mediated; transfection, electroporation, transformation of protoplasts.

Module:6 Selection and screening of recombinants 4 hours

Genetic selection, identification of recombinants- insertional inactivation, screening for recombinants
lac selection, screening for blue white colonies; Marker genes- endogenous selectable marker
genes, dominant selectable marker genes, reporter genes. DNA sequencing by enzymatic and
chemical methods: Maxam and Gilbert procedure, Sanger’s chain-termination method; shotgun
approach, clone contig approach to genome sequencing, automated pyrosequencing.
Module:7 Techniques & Applications of genetic 7 hours
Polymerase chain reaction
engineering (PCR) and its applications; Types of PCR and their applications-
multiplex, nested, inverse, real time, quantitative, hot-start, touchdown; Methods of nucleic
acid hybridization-
Southern, Northern and Western blotting techniques; Labelling of DNA, RNA and proteins by
radioactive isotopes, non-radioactive labelling, autoradiography. Site-directed mutagenesis, exon
cloning, chromosome walking and jumping, EMSA, RNase A protection assay, DNAse I
foot printing assay, microarrays,
Chromatin immunoprecipitation assay. Applications: Gene cloning in medicine (Insulin, Blood
clotting factor VIII); Genetic engineering for human gene therapy; Industrial applications of rDNA
technology.

Module:8 Contemporary Issues: 2 hours

Industry expert lectures on contemporary issues
Total Lecture hours: 30 hours

Text Book(s)
1. Principles of Gene Manipulation and Genomics, Seventh Edition (2014) S.B. Primrose,
S.B. and
2. Gene Twyman,
R.M. Cloning and
Blackwell
DNA Analysis:
PublishingAn
Company,
Introduction.
Oxford,
Seventh
UK. Edition (2016) T.A. Brown,
Wiley
Reference Books
Blackwell.
1. Molecular Cloning. A Laboratory Manual. Volume 1-3. Fourth Edition (2013) Michael R
Green and
Mode of Evaluation: CAT / Assignments / FAT
Joseph Sambrook. Cold Spring Harbor Laboratory Press, New York, USA.
Recommended by Board of Studies 03-08-2017
Approved by Academic Council No. 46 Date 24-08-2017

[Link]. Biotechnology Page 48

Course code Course title L T P J C
BST5011 Bioinformatics 2 0 0 4 3

Pre-requisite Syllabus version

v.1.1
Course objectives:
1. Spell the concepts and applications of Bioinformatics
2. Demonstrate the bioinformatics skills to solve biological problems
3. Model the Biological databases
4. Construct and evaluate open access biological databases and sequence alignment algorithm
5. Demonstrate about the heuristic algorithms, phylogenetic analysis and structure prediction
6. Simplify the knowledge on the latest trends in new drug discovery
Expected Course Outcome:
1. Infer the solutions to basic bioinformatics problems discuss the use of bioinformatics in
addressing a range of biological questions
2. Distinguish how bioinformatics methods can be used to relate sequence, structure and function
3. Categorize central bioinformatics data and information resources
4. Model the principles and algorithms of pairwise and multiple alignments, and sequence database
searching
5. Relate pattern matching in bio molecular sequences
6. Justify how evolutionary relationships can be inferred from sequences (phylogenetics)
7. Experiment with basic principles of hidden Markov models and their application in sequence
analysis.

Student Learning Outcomes (SLO): 2 ,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Bioinformatics database and Resources: 4 hours
File formats (Genbank, Uniprot, PDB), NCBI, RCSB, DDBJ, GenBank, Uniprot-KB,
PDB,KEGG, EMP, ExPASY server, GCG utilities, Sequence formats, R package, EMBOSS,
Genome Browser, iGEM, Bioconductor

Module:2 Sequence analysis: 4 hours

Measure of sequence similarity; identity and homology, Concept of homologs, orthologs and
paralogs. Scoring matrices- PAM, BLOSUM, Sequence Alignment-Needleman Wunsch
algorithm, Smith Waterman algorithm, Use of sequence alignments for analysis or nucleic acids
and protein sequence, NGS analysis

Module:3 Taxonomy and Phylogeny: 4 hours

Data types used in taxonomy and phylogeny, Phylogenetic trees, Algorithms like maximum

[Link]. Biotechnology Page 49

parsimony, UPGMA, 16s rRNA typing, Probabilistic models of evolution.
Module:4 Drug Design: 4 hours
Target identification and validation, Analog based drug design- Pharamcophore and QSAR,
Docking, De novo drug design-Fragment placements, sequential grow, connection methods

Module:5 Big Data Informatics: 4 hours

Data processing, Parallelism in storage, Open source big data tools, Bioinformatics Challenges

Module:6 Structural Bioinformatics: 4 hours

Conceptual model of protein structure, protein structure prediction and modelling – Homology
Modeling, Threading, Ab initio- Protein Structure Visualization, Comparison and Classification,
CAPRI, Protein Structure Initiative, Computational Proteomics

Module:7 Applications: 4 hours

Virtual screening, Pharmacogenomics, Antisense Technology

Module:8 Contemporary issues: 2 hours

Industry expert lecture on contemporary issues

Total Lecture hours: 30 hours

Text Book(s)
1. Bioinformatics- a practical guide to the analysis of Genes and Proteins by Baxevanis, A.D.
and Francis Ouellellette, B.F., Wiley India Pvt Ltd. 2016.
Reference Books
1. Bioinformatics- sequence and Genome analysis by Mount D., Cold Spring Harbor Laboratory
Press, New York, 2014.
2. Bioinformatics- From Genomes to Therapies, Vol 1-3, Wiley Inc., 2016.

Mode of Evaluation: CAT / Assignments / FAT

Recommended by Board of 03-08-2017
Studies
Approved by No. 46 Date: 24-08-2017
Academic Council

[Link]. Biotechnology Page 50

 PROGRAMME ELECTIVES

[Link]. Biotechnology Page 51

Course code L T P J C
BST5005 Medical Diagnostics 3 0 0 3 3
Pre-requisite Nil Syllabus version
v 1.1
[Link] the knowledge about various types of specimen received in the diagnostic laboratory
and the purpose for which they are sent
[Link] Interpret the procedures carried out in different laboratories
[Link] illustrate the molecular diagnostic and imaging tools to assist the clinical diagnosis.

1. To justify the importance of diagnostic tools and different laboratories

2. To summarize the basis of clinical and haematological tests
3. To infer clinical significance of urine and faeces analysis
4. To construct the techniques in histopathological laboratory.
5. To organize the knowledge of common infectious and non-infectious diseases
6. To explain the basis of common imaging techniques and their applications
Student Learning Outcomes (SLO): 2 ,11
2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Introduction: 4 hours
History of diagnostics, importance of medical diagnostics, diagnostic tools.

Module 2 Fields of medical diagnostics 5 hours

Histopathology, serology, biochemistry, haematology and microbiology.

Module:3 Hematological investigation 6 hours

Blood composition, blood sample collection and smear preparation, Differential cell counts –
RBC, WBC, Platelets, hemoglobin estimation, erythrocytic sedimentation rate (ESR), Blood
platelet count by hemocytometer, and testing of blood glucose using glucometer.

Module:4 Urine and feces analysis: 6 hours

Formation and composition of urine, Physical characteristics of urine, sample collection, detection
of abnormal constituents. Fecal formation and stool sample preparation for microscopic
evaluation.

Module:5 Histopathology: 6 hours

Biopsy sample collection, tissue sample processing, embedding, sectioning, staining and
mounting, histopathological techniques – Hematoxylin & Eosin (H&E) staining and
immunohistochemistry.

[Link]. Biotechnology Page 52

Module:6 Infectious diseases and Non - infectious 7 hours
Diseases
Types, causes and molecular basis of the disease, symptoms and diagnosis of infectious disease
(Example: tuberculosis, typhoid and malaria) and non- infectious disease (Example: diabetes
(Type I and II), rheumatoid arthritis)

Module:7 Diagnostics using medical imaging 9 hours

Diagnostic radiography, Electrocardiogram (ECG), Ultrasound, Sonography, Positron emission,
tomography (PET), Magnetic resonance imaging (MRI) and Computerized tomography (CT)
Scan. Applications of medical imaging in the detection and diagnosis of cancer, Recent trends in
the field of medical diagnostics, Guest lectures by experts on recent updates

Module:8 Contemporary issues: 2 hours

Expert lecture from clinician, academician and industry

Total Lecture hours: 45 hours

Text Book(s)
1. Guyton A.C. and Hall J.E. 2010, Textbook of Medical Physiology, Saunders
Reference Books
1. Prakash, G. 2012, Lab Manual on Blood Analysis and Medical Diagnostics, S. Chand and Co.
Ltd.

2 Macleod J. 2013, Davidson’s Principles & Practice of Medicine: A textbook for students and
doctors’ 22nd Edition. Churchill Livingstone.
Mode of Evaluation: CAT / Assignment /Quiz / Project/FAT
Project: „J‟ component
Recommended by Board of Studies 03-08-2017
Approved by Academic Council No. 46 Date 24-08-2017

[Link]. Biotechnology Page 53

Course code Course title L T P J C
BST5006 Tissue Engineering and Regenerative Medicine 3 0 0 0 3
Pre-requisite Nil Syllabus version
v.1.1
Course Objectives:
1. Relate the use of Biomaterials and cell culture in Tissue Engineering
2. Illustrate the significance of scaffold design
3. Distinguish the role of stem cell technology in TE and RM.
Expected Course Outcome:
1. Outline the biological requirement for designed tissue engineering systems
2. Categorize the biomaterials suitable for intended tissue applications
3. Summarize the drug delivery modes in tissue engineering
4. Justify and fabricate the scaffolds for growing biological materials using conventional methods
and advanced manufacturing technologies
5. Model tissue regeneration process implying the stem cells and growth factors
6. Design and construct artificial organ upon patient’s need.

Student Learning Outcomes (SLO): 2,11

2. Having a clear understanding of the subject related concepts and of contemporary issues 11.
[Link] interest in lifelong learning
Module:1 Introduction to Tissue Engineering and 3 hours
Regenerative Medicine:
Introduction to Tissue Engineering and Regenerative Medicine. Principles of tissue development
and organization. Biology of Regeneration, Basis of Regenerative Medicine, Strategies of
Regenerative Medicine

Module 2 Mammalian cell sources, cell 6 hours

culture conditions:
Mammalian Cell sources, cell culture conditions: cell container, medium, protocols, 3D culture,
Extracellular matrix. Introduction to Stem cells, cell reprogramming, SCNT

Module:3 Biomaterials Classification: 6 hours

Bioinert, biodegradable, bioactive. Types of biomaterials, smart material, drug delivery, cell-
material interaction

Module:4 Scaffold fabrication 6 hours

Scaffolds for Tissue Engineering, Classification of scaffold materials - examples, criteria for ideal
scaffold, control of architecture, Scaffold design and fabrication techniques

Module:5 Clinical applications: 8 hours

Musculoskeletal tissue engineering, Cardiovascular tissue engineering, Neural tissue engineering.

[Link]. Biotechnology Page 54

Skin tissue engineering, Expert lecture or Review of present status of clinical tissue engineering

Module:6 Bioartificial Organs 8 hours

Artificial tissue and artificial skeleton. Three dimensional cell culture and tissue growth, 3D
printing of tissue, cells and organs. Bioartificial heart, Bioartificial kidney.

Module:7 Tissue regeneration: 6 hours

Tissue Regeneration Driven by Growth Hormones, Stem Cells as source in regeneration of
tissues, Therapeutic Applications: Tissue Therapy, Drug-vaccine-viral delivery in RM.

Module:8 Contemporary Issues: 2 hours

Industry expert lectures on contemporary issues

Total Lecture hours: 45 hours

Text Book(s)
1. Principles of Regenerative Medicine , Anthony Atala, Robert Lanza, James A. Thomson,
Robert M. Nerem, 2010 Academic Press
2 Principles of Tissue Engineering (Fourth Edition) Robert Lanza, Robert Langer, Joseph
Vacanti, 2014, Academic Press
Reference Books
1. Tissue Engineering for Artificial Organs: Regenerative Medicine, Smart Diagnostics and
Personalized Medicine, Anwarulhassan, 2017, Wiley – VCH Press

2. Regenerative Medicine and Tissue Engineering. Jose A. Andrades, InTech, 2013

Mode of Evaluation: CAT / Assignments / Quiz / FAT

Recommended by Board of Studies 03-08-2017
Approved by Academic Council No. 46 Date 24-08-2017

[Link]. Biotechnology Page 55

 Course code Course title L T P J C
BST5007 Medical Biotechnology 3 0 0 0 3
Pre-requisite Nil Syllabus version
v. 1.1
Course Objectives:
1. Demonstrate the advances in medical biotechnology field
2. Compare the biology, diagnostics and treatment opportunities for various diseases
3. Critically analyze the techniques that can be used for diagnostics and treatment

Expected Course Outcome:

1. The students will be able to define the basic of various diseases.
2. The students will be able to interpret the prognostic and diagnostic methods and techniques to identify
markers.
3. The students will be able to conclude a clear picture on various biomolecules and molecular
therapeutic approaches
4. The students will be able to compile the prophylaxis method, medical information database
[Link] students will be able to model the treatment strategies and how to screen compounds for a
particular bioactivity.

Student Learning Outcomes (SLO): 2,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 The Biology of disease 8 hours
Infectious diseases, inflammatory diseases, the molecular basis of Senescence and cell death,
Neurodegenerative diseases and Chromosome abnormalities.

Module:2 Prognosis and Diagnostics 6 hours

Immunodiagnostics, genetic diagnosis, protein markers and identification of disease specific
markers, microarrays, automated workstations, genetic testing-neonatal screening.

Module:3 Therapeutics 6 hours

Monoclonal antibodies, Therapeutic proteins in the treatment of various diseases, Cytokines,
hormones, gene therapy, Basic approaches and applications of gene therapy in cancer and genetic
disorders.

Module:4 Prophylaxis 6 hours

Vaccines and production, cancer vaccines, medical information databases, prebiotics and probiotics.

Module:5 Disease treatment strategies 6 hours

Rational drug design, important criteria in drug designing, High-throughput compound screening,
various new developments in drug delivery and antisense RNA technology

[Link]. Biotechnology Page 56

 Module:6 Clinical approach 6 hours
Animal models of human diseases including, Cancer, Neurodegenerative diseases and diabetes,
Clinical Trials.

Module:7 Ethics and regulations 5 hours

Ethics and regulations in clinical research, licensing procedures in India, Intellectual property rights
and patents in biotechnology

Module:8 Contemporary issues 2 hours

Recent trends in medical biotechnology lectures by industry experts

Total Lecture hours: 45 hours

Text Book(s)
1. Medical Biotechnology, Bernard R. Glick, T.L. Delovitch, Cheryl L. Pattern, ASM Press, 2014.
2. Biotechnology and Medical Sciences, Firdos Alam Khan, CRC Press, 2014.
Reference Books
1. Medical Biotechnology, Firdos Alam khan, Academic Press, 2013.
2. An Introduction to human diseases: Pathology and Pathophysiology correlations. Leonard V.
Crowley, 2012.
3. Apoptosis: Physiology and Pathology, John C Reed and Douglas R. Green, Cambridge
University Press, 2011.
Mode of Evaluation: CAT / Assignments / Quiz / FAT

Recommended by Board of Studies 03-08-2017

Approved by Academic Council No.46 Date 24-08-2017

[Link]. Biotechnology Page 57

 Course code Course title L T P J C
BST5008 Industrial Biotechnology 2 0 2 0 3

Pre-requisite Nil Syllabus version

v. 1.1
Course Objectives:
[Link] insight on the various aspects biological systems and harnessing of biomolecules
[Link] various optimization parameters and strain improvement
[Link] the practical knowledge on bioreactors and fermentation process

Expected Course Outcome:

1. Discover the development of industrial biotechnology
2. Formulate the knowledge on mathematical solutions for industrial problems
3. Demonstrate the optimization parameter and sterility maintenance
4. Experiment with the techniques involved in strain improvement
5. Infer the importance of bioreactor and the processes of fermentation along with economics and cost
benefit
6. Construct a novel biosystems for upstreaming and down streaming process
Student Learning Outcomes (SLO): 2 ,11

2. Having a clear understanding of the subject related concepts and of contemporary issues.
[Link] interest in lifelong learning
Module:1 Introduction to products and 4 hours
fermentation processes:
Historical overview; chronological development; recent advances

Module:2 Process calculations: 3 hours

Dimensions, variables and system of units; Basics in chemical and stoichiometric
calculations and dimensional analysis; Ideal gas law, ideal mixtures and solution, Dalton’s Law
of Additive pressures, Amagot’s Law of Additive volumes. Mass transfer and diffusion
limitations. Heat transfer- equipment,mechanism and calculations; Thermal death kinetics

Module:3 Media standardization and sterilization: 4 hours

Nutrient requirements and their optimization (classical and statistical); Sterilization: physical and
chemical treatment; modes of sterilization operation: batch and continuous; sterilization in
industries.

Module:4 Strain Improvement: 5 hours

Kinetics of growth, growth curve; mutation, selection and genetic recombination. Case studies
in strain improvement for production of organic acids and amino acids.

Module:5 Bioreactor operation& monitoring: 4 hours

[Link]. Biotechnology Page 58

 Basic instrumental components of a bioreactor; bioreactor configurations - types of bioreactors,
modes of operation of bioreactors – continuous stirred tank reactor, batch reactor, fed batch reactor,
stirred tank reactor with recycle and reactors in series; reactors for plant cells and animal cells,
immobilized reactors.
Module:6 Monitoring of Bioprocess: 4 hours
Process variable measurement and control – temperature, gas and liquid flow, pressure, agitator,
pH, biomass, foam formation, weight, dissolved oxygen, inlet and exhaust gas, redox and
carbon dioxide; probes for sampling and control; sensors; on-line data analysis for
measurement of parameters; basics of computer application in bioprocess.

Module:7 Downstream processing, economics and ethics: 4 hours

Technical feasibility, process development, Environmental safety and Health considerations,
Marketability of the product, Capital investments, Plant overheads and depreciation, Profitability
Analysis, Patents and
Copyrights
Module:8 Contemporary issues: 2 hours
Industry expert lectures on contemporary issues

Total Lecture hours: 30 hours

Text Book(s)
1. Industrial Biotechnology: Sustainable Growth and Economic Success, Eds. W
Soetaert, EJ Vandamme, 2010, Wiley-VCH
2. Shuler ML and Kargi F., Bioprocess Engineering: Basic concepts, 2nd Edition,
Prentice Hall,Engelwood Cliffs, 2017.

Reference Books
1. Stanbury RF and Whitaker A., Principles of Fermentation Technology, Butterworth-
Heinemann,
List of Challenging2016
Experiments (Indicative)
1. Statistical optimization of media: Plackett-Burman Design and RSM 2 hours
2. Strain improvement of Aspergillus niger 2 hours
3. Setting up shake flask culture; growth curve & determination of growth by 2 hours
Spectroscopy
Analysis of growth, C & N and production profile
4. 2 hours
5. Demonstration of Bioreactor (Application demo) 2 hours
6. Optimization of parameters for scale up of (3) based on results of (4) 2 hours
7. Extraction of products/ metabolites 2 hours
8. Purification by dialysis 2 hours
9. Purification by chromatographic procedures 2 hours
10. Characterization by relevant bio-analytical technique 2 hours
Total Laboratory Hours 24 hours
Mode of Evaluation: CAT / Assignments / Quiz / FAT
Recommended by Board of Studies 03-08-2017
Approved by Academic Council No. 46 Date 24-08-2017

[Link]. Biotechnology Page 59

Course code Course title L T P J C
BST6001 Cancer Biology and Therapeutics 2 0 0 4 3
Pre-requisite Syllabus version
v.1.1
Course Objectives:
1 Building the ability to be socially intelligent with good SIQ (Social Intelligence Quotient) and
EQ (Emotional Quotient)
2 Having a clear understanding of the subject related concepts and of contemporary issues
3 Developing problem solving ability- solving personal and social issues
4 Combining a clear understanding of professional and ethical responsibility in facing the
outcome and treating the disease
5 Adopting critical thinking and innovative skills
6 Having a good digital footprint and Virtual Collaborating ability
7 Solving problem by computational thinking (Ability to translate vast data in to abstract
concepts, and to understand database reasoning)
Expected Course Outcome:
1. Outline and summarize the process of pathology, progression of cancer
2. Identify the etiological mechanism responsible for cancer, available diagnostic, treatment
methodologies
3. Make the students to discover with the products or processes which are ultimately aimed in
preventing or treating cancer cost effectively
4. Appraise the utility of different analytical techniques that can be used to study the outcome of
the disease
5. Construct Omics based methodologies for qualitative and qualitative analysis for the prediction
and application for the treatment

Student Learning Outcomes (SLO): 2 ,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Overview and Origin of cancer Hours 4
Introduction, Defining cancer, Current hallmarks of cancer, Carcinogens (environmental pollutants,
chemical, viral, radiation), cellular transformation into cancer, Methods in studying carcinogenesis, Cancer
as a complex metabolic disorder

Module 2 Tumorigenesis, Oncogene activation, Tumour Hours 4

suppressor inactivation
Discovery of oncogenes and genetic abnormalities, Multi-step tumorigenesis process, Role of tumor
suppressor genes inactivation and oncogene activation in tumor pathogenesis

Module:3 Cell cycle Dysregulation Hours 4

Cell cycle and regulatory proteins, checkpoint measures during DNA damage (involvement of Rb, p53
proteins), Cell cycle modulation in cancer. Apoptosis and Altered Signalling Pathways- Apoptosis,
Mechanism, defective apoptotic/cell proliferative mechanisms leading to cancer: Pathways regulating

[Link]. Biotechnology Page 60

tumor initiation and/or its progression

Module:4 Angiogenesis and Metastasis Hours 4

Angiogenesis, Hypoxia, Mechanism, Current targeting strategies, Metastasis - Proposed theory and
mechanisms, epithelial to mesenchymal transition, interaction of cancer cells with normal cells, clinical
interventional measures.

Module:5 Cancer Stem cells, Cell of Origin, Hours 5

Chromosomal Abnormalities
Cancer Stem cells, clinical implications and targeting CSCs, Insight into genomic instability, tissue
specificity and cells of origin, mouse models in studying cancer origin, Senescence, immortalization,
ageing andTelomere dysregulation in cancer, Chromosomal Modifications, Cancer Epigenetics

Module:6 Cancer Therapeutics Hours 4

Chemotherapy, Surgery and Radiation Therapy; Mechanism, FDA approved Anticancer drugs and FDA
approval procedures, Cancer immunotherapy, Therapeutic Screening: Role of cell lines, chemically
induced models, knockout mouse model, Xenograft models, patient-derived xenografts (PDXs), Futuristic
cancer therapeutics approaches

Module:7 Cancer Diagnosis, Risk assessment, Prognosis Hours 4

Screening methods; conventional and new visualization Techniques - Histopathology, X-ray, CT-Scan,
MRI, PET; Molecular Screening and early detection - Cytogenetics, molecular cytogenetics and array
based techniques;Cancer markers: promises and challenges, genomic and proteomic technologies in
targeting cancer.

Module:8 Cancer Informatics (Industry Expert‟s Hours 1

Lectures):
High throughput sequencing technologies to detect genetic alterations in cancer, Current
approaches in genomics and proteomics contributing towards cancer prognosis and early
detection, Cancer Databases: The Cancer Genome Atlas, METABRIC

Total Lecture hours: Hours 30

Text Book(s)
1. Text Books and articles: The Biology of Cancer – Robert Weinberg. Edition – 2nd
ISBN:9780815342205 - 2013

Reference Books
1. 1. Cancer Sourcebook, Edited by Edited by Karen Bellenir, Omnigraphics, Inc., 2011, ISBN
978-0- 7808-1145-4

[Link]. Biotechnology Page 61

2. 2. Cancer cell signalling / edited by Amanda Harvey, John Wiley & Sons, Ltd, 2013, ISBN
978-1-119- 96757-6 (pbk.)

3. 3. The Tumor Microenvironment, Edited by Rebecca G. Bagley, 2010, ISBN 978-1-4419-

6614-8 eISBN 978-1-4419-6615-5, DOI 10.1007/978-1-4419-6615-5, Springer New York
Dordrecht Heidelberg London

Mode of Evaluation: Use of technology in teaching, lecture by industry experts, Written

examinations, Projects and assignments
Recommended by Board of Studies 03.08.2018
Approved by Academic Council No.46 Date 24.08.2017

[Link]. Biotechnology Page 62

Course code Course title L T P J C
BST6002 Stem Cell Biology 3 0 0 0 3

Pre-requisite Knowledge of cell biology is desirable. Syllabus version

v. 1.1
Course Objectives:
1. Students will recall and relate the facts and concepts pertaining to this course.
2. Students will combine the cellular, molecular and epigenetic aspects pertaining to stem cells
3. Students will discover the importance of optimization of stem cell culture conditions for
effective stem cell therapy

Expected Course Outcome:

1. To Compare different types of stem cells including induced pluripotent stem cells and the extent
of their plasticity as well as the basics
2. To Improve the mechanistic interpretation of the cellular and molecular players in stem cell
self-renewal, proliferation and differentiation
3. To better assess and interpret the basis for the origin, metastatic potential, cause for relapse and
the state-of-the-art in cancer treatment strategies (at the cancer stem cell level)
4. To be able to illustrate and demonstrate the use of appropriate media components for the
proliferation and differentiation of the different types of stem cells
5. To be able to prioritize and rephrase the current state-of-the-art issues and challenges in terms
of stem cell therapy

Student Learning Outcomes (SLO): 2 ,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Introduction to stem cell Biology 5 hours
Basic definitions and terminologies; Self-renewal and pluripotency; Quiescence vs senescence;
Embryonic and adult stem cells; Stem cell debate, politics and ethics

Module:2 Stem cell niche, epigenetic regulation of stem 6 hours

cell fates and nuclear reprogramming
Stem cell niche – Role and molecular characterization; Epigenetic regulation – Histone and DNA
modifications, ATP-dependent chromatin modeling; Nuclear reprogramming – Somatic cell
nuclear transfer and induced pluripotent stem cells

Module:3 Signal transduction pathways 6 hours

Canonical and non-canonical Wnt signaling; Sonic hedghog signaling; TGF- and FGF signaling;
BMP and Notch signaling.

Module:4 Heamatopoietic stem cells 6 hours

HSC subpopulations and the niche, lineage commitment; HSC therapy success stories

Module:5 Mesenchymal stem cells 6 hours

[Link]. Biotechnology Page 63

 Immunomodulatory role and engraftment potential; Therapeutic uses

Module:6 Cancer stem cells 6 hours

Stem cell models; Cancer metastasis and relapse; Novel cancer drugs

Module:7 Stem cell culture protocols 6 hours

Feeder-dependent protocol; Feeder-independent protocol; Feeder-free protocol

Module:8 Stem cell therapies 4 hours

Advantages and constraints; Current status of stem cell-based therapies

Total Lecture hours: 45 hours

Text Book(s)
1. Essentials of Stem Cell Biology. 2nd edition. (2009). Academic Press USA.

2. Stem Book. [Link]

3. Recent peer-reviewed papers.

Reference Books
1. Yanhong S., Dennis C.O. (Ed.) Stem Cell Research & Therapeutics. Springer. New Delhi.
2010.
2. Vemuri M., Stem Cell Assays. Humana Press, NJ. 2010.
3. Newton [Link] Cell Research. Viva Books Pvt. Ltd. Delhi. 2008.
Mode of Evaluation: Use of technology in teaching, lecture by industry experts, Written examinations,
Projects and assignments
Recommended by Board of Studies 03.08.2017
Approved by Academic Council No. 46 Date 24.08.2017

[Link]. Biotechnology Page 64

Course code Course title L T P J C
BST6003 Clinical and Translational Research 3 0 0 0 3
Pre-requisite Nil Syllabus version
v.1.1
Course Objectives:
1. To construct an overview and examples of how basic science and clinical observations lead to
translational research.
2. To motivate the next generation of investigators who will lead cutting edge clinical research
into the future.
3. To illustrate the students about the safety, risk assessment and adverse reactions to drug in order
to develop problem solving capabilities.
Expected Course Outcome:
1. Rephrase the general ethical frameworks, along with specific ethical principles underpinned by
those frameworks, in the context of current developments in biotechnology, clinical practice, and
the ethical oversight of research on humans.
2. Build methodologically robust and statistically valid clinical research protocols.
3. Judge the clinical research procedure that complies with highest national and international legal,
regulatory and scientific standards.
4. Translate, evaluate and enhance clinical research protocols, ensuring the highest quality
research output.
5. Outline the principles of good clinical practice, how to conduct a clinical trial, how drugs are
developed and how to manage regulatory documents.
6. Combine the needs for patients, academic partners and industry to complete valid clinical
research programmes.

Student Learning Outcomes (SLO): 2,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Introduction: 3 hours
Definitions, bench-to-bedside concepts, debate, politics and ethics

Module 2 Regulations and regulatory bodies: 5 hours

Regulations related to the use of drug, gene and cell-based products, Regulatory governance in the
US, Europe and Asia

Module:3 Good laboratory practices(GLPs) and Good 7 hours

Manufacturing practices (GMPs):
Biosafety levels, quality assurance, SOPs and documentations, GLPs associated with drug, gene
and cellbased products, Facility organization, personnel training, quality assurance,
documentations, manufacturing and validation, GMP associated with drug, gene and cell-based
products

Module:4 Translational research: 8 hours

Bench to bedside, T1, T2, T3 and T4 phases, Treatment approaches using Imaging tools,

[Link]. Biotechnology Page 65

Nanotechnology, Small molecules (Chaperones), Gene-based therapies, Innovative services and
Informatics tools; Health Street model.

Module:5 Human Based Systems for Translational 8 hours

Research:
Translational Research in Pharmacology and Toxicology Using Precision-Cut Tissue Slices,
Modelling the Human Respiratory System: Approaches for in vitro Safety Testing and Drug
Discovery, Survival analysis, “Body-on-a-Chip’ Technology and Supporting Microfluidics,
Human micro-dosing.

Module:6 Preclinical trials and Clinical trials: 7 hours

Small and large animal models, animal facility clearance, care and maintenance of animals,
Clinical trials- Phase – I, II, III and IV clinical trials, Investigational new drug/device applications.

Module:7 Risk assessment, mitigation and emerging 5 hours

topics
Safety risk and adverse reactions to drug, gene and cell-based products; Consequences and
mitigation, Drug scenario in India, Rational use of medicine in India. Pharmacovigilance –
challenges in India, Standard treatment guidelines and essential medicine list, Invited talks by
industry experts, policy makers and medical practitioners.

Contemporary issues: 2 hours

Module:8
Industry expert lectures on contemporary issues

Total Lecture hours: 45 hours

Text Book(s)
1. Clinical and Translational Science: Principles of Human Research, 2nd Edition (2016). Edited
by David Robertson, Gordon H. Williams.
Reference Books
1. Good Manufacturing Practices for Pharmaceuticals, 6th edition (2016), edited by Joseph D.
Nally.

2. Various WHO guidelines and various e-reference material from regulatory authorities and
scientific bodies
Mode of Evaluation: Continuous assessment and Final Assessment test.
Recommended by Board of Studies 03-08-2017
Approved by Academic Council No. 46 Date 24-08-2017

[Link]. Biotechnology Page 66

Course code Course title L TP J C
BST6004 Forensic Science and Technology 2 0 0 4 3
Pre-requisite Syllabus version
v.1.1
Course Objectives:
1. Know and explain the functioning of national and international legal systems
2. Identify the steps to be taken during any investigations
3. Familiarise the students with available technology, shortage, and improvements in evidence
analysis. Thus simplify the products or processes ultimately aimed at speedy and in cost effective
manner
4. Interpret and judge different analytical techniques to be used during evidence analysis5.
5. Compile the databases for qualitative and qualitative analysis, and for the prediction during the
investigations
Expected Course Outcome:

[Link] a clear outline about the subject related concepts and of contemporary issues
2. Develop an ability to be socially intelligent, with good SIQ (Social Intelligence Quotient) and
EQ (Emotional Quotient)
3. Utilizing computational thinking (Ability to translate vast data in to abstract concepts and to
understand database reasoning)
4. Perceived with the problem solving ability, thereby solving the individual and social problems
5. Building a Virtual Collaborating ability
6. Combining the critical thinking and innovative skills

Student Learning Outcomes (SLO): 2,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Introduction to Forensic Science 5 hours
History and Significance, Experts involved and procedures in crime scene investigative, Forensic
laboratories: National and Global laboratories, Body farms: Recent advances.

Module 2 Crime Scene Investigation, and 4 hours

Instrumentation:
Evidences: Physical Evidence, collection protocols, Documentation, Chain of Custody,
Instrumentation in Forensic Analysis.

Module:3 Forensic Fingerprinting 6 hours

Principle, Types, Fingerprint lifting techniques, Modus Operandi Sheet preparation, Fingerprint
Recorders: Biometric system in detecting individual variation, Optical, Capacitance-based and
other types of fingerprint recorders

Module:4 Impression Evidences, Documents, and other 6 hours

Evidences in Forensic Analysis
Impression based evidence: Principle, Tool markings, Tire, Footwear markings and associated databases,
Hand writing analysis, Question documents, Polymers and Fiber.

[Link]. Biotechnology Page 67

Module:5 Forensic ballistic procedures 5 hours
Types, application, procedures: internal, external and terminal ballistics, and identification of firearms,
databases in ballistic analysis.

Module:6 Serology and Toxicology in Forensic Evaluation 6 hours

Serological analysis (blood, saliva, semen etc), Blood Splatter- Origin of impact study, Abusive Drug
types, CSA- schedules, Poisons and analysis, Pathology and DNA fingerprinting in Forensics- Time of
death analysis; Entomology and pathology in death analysis, Bite-mark analysis, Forensic Medicine, DNA
fingerprinting: RFLP and PCR (VNTR, STR-CODIS) based profiling

Module:7 Forensic Photography and Digital Criminalistics: 6 hours

SLR-camera, Digital camera, CCTV in forensic analysis, Camera techniques for evidence visualization,
Forensic Facial Reconstruction, Cyber Forensics: Computer, Mobile phone data analysis, Ethical hacking,
drones, remote surveillance in forensic investigations, and Corporate crimes, deception detection tests
(DDT): polygraph, narco-analysis and brain-mapping.

Module:8 Forensic and Legal proceedings in India 2 hours

Legal proceedings in forensics, CSI in India, and Case studies.

Total Lecture hours: 46 hours

Text Book(s)
1. Criminalistics: An Introduction to Forensic Science, 11/E, Richard Saferstein, ISBN-10:0133458822 •
ISBN-13: 9780133458824, 2015 • Prentice Hall.

Reference Books
1. Forensic DNA Typing, 2nd Edition, Biology, Technology, and Genetics of STR Markers, J Butler,
2005, Imprint: Academic Press, eBook ISBN: 9780080470610, Print Book ISBN: 9780121479527,
Pages: 688
Introduction to Criminalistics: The Foundation of Forensic Science, 2009, by Barry A.J. Fisher,
2. William J. Tilstone, Catherine Woytowicz, Elsevier Academic Press USA, 2009.

3. Hendry Lee’s Crime Scene Handbook, H.C. Lee, T. Palmbach, M.T. Miller (Academic Press),
Published: June 2001, ISBN: 978-0-12-440830-2
Mode of Evaluation: Assignments, Continuous assessment tests and Final assessment test.

Recommended by Board of Studies 03.08.2017

Approved by Academic Council No.46 Date 24.08.2017

[Link]. Biotechnology Page 68

Course code Course title L T P J C

BST6005 Pharmacology and Toxicology 3 0 0 0 3

Pre-requisite Syllabus version
v. 1
Course Objectives:
1. Enhancing the basic pharmacological principles within the field of pharmacokinetics and -
dynamics
2. Compare different classes of receptors which interact with drugs, and describe intracellular
transduction mechanisms coupled to some of these receptors
3. Recognize some of the common manifestations of poisonings as they may present clinically

Expected Course Outcome:

1. The students may acquire the knowledge on study of drug and routes of administration
2. Analyse drug distribution between the compartments of the body and understand the factors
affecting the pharmacokinetics of drug in its therapeutic regimen.
3. Acquire knowledge on molecular & biochemical aspects of drug actions, receptors, drug
receptor interactions, factors modifying drug effects.
4. Appreciate the toxicity caused when chemicals interfere with physiologic functions;
5. Apply their knowledge of toxicology to the methods used to detect and monitor toxicity
6. The students get to know alternate methods / models for assessing toxicity
Student Learning Outcomes (SLO): 2 & 11
2. Having a clear understanding of the subject related concepts and of contemporary issues 11.
[Link] interest in lifelong learning
Module: 1 General Pharmacology and Pharmacokinetics 6 hours
History of Pharmacology. Routes of Drug administration, Absorption, Distribution and
Metabolism. Elimination of drugs: Concept of renal clearance and excretion of drugs –
biological half – life, area under curve. Bio-availability of drug products.

Module: 2 Pharmacodynamics 6 hours

Introduction, Receptor theory, Enzyme interactions, physico-chemical interactions. Ionic fluxes
second messengers and G proteins. Dose-effect relationships of drugs (graded and quantal
response) therapeutic index, potency and efficacy competitive and non-competitive antagonists &
agonists. Mechanism of action of general anesthetic agents and analgesic. Mechanisms of adverse
drug effects.

Module: 3 Design and Development of Drugs 6 hours

a) Drug discovery process: Principles, Techniques and Strategies used in new drug discovery.
Regulations for laboratory animal care and ethical requirements. b) Bioassays: Basic principles of
bioassays, official bioassays and experimental models
c) Pre-clinical and clinical models employed in the screening of new drugs

Module: 4 Clinical Pharmacology 6 hours

a) Definition and scope of clinical pharmacology, Evaluation of drugs in experimental and
clinical trials. b) Drug therapy monitoring and differences in drugs response. b) Patient

[Link]. Biotechnology Page 69

counseling and interviewing techniques, Improving patient compliance and patient monitoring.

Module: 5 Molecular pharmacology 6 hours

a) Molecular mechanism of drug action: Receptor occupancy and cellular signalling systems such
as G-proteins, cyclic nucleotides, calcium and phosphatidyl inositol. Ionic channels and their
modulators. b) Endogenous bioactive molecules: Different kinds of endogenous bioactive
molecules and its roles (Cytokines, neuropeptides, steroids, nitric oxide, phosphodiestrase
enzyme and protein kinase C).

Module: 6 Toxicology 5 hours

a) History and Exposure routes – Food, Water, Air and Skin. b) Alternative to animal screening
procedure. Models of study drug metabolism. Adverse drug reactions and drug interactions.

Module: 7 Cytotoxicity 5 hours

a) Molecular mechanisms of Cell death- reactive intermediates and detoxification of radicals.
Methods of establishing level of necrosis. Determining covalent binding and LPO.
Determination of LD50, ED50, and TD50. b) Genetic toxicity – Mutagenicity, Biological
relevance of mutations. Types of mutations – Gene, Structural and Genome mutations.
Mutagenicity tests.

Module: 8 Metals toxicology 5 hours

a) Metal metabolism and toxicity. Cellular and Molecular Mechanism of Metal toxicity. b)
Nutrition and toxicology – Influence of Dietary substance and Nutrients in Xenobiotics.
Inhalation toxicology – Deposition of Inhalated Materials (Gases, Vapors & Particles) its
metabolism

Total Lecture hrs 45 hours

Mode : Use of technology in teaching, lecture by industry experts
Evaluation : Written examinations, Projects and assignments
Text Books and articles:
1. Humphrey p. Rang. Rang & amp Dale’s Pharmacology, 7th edition 2011.
2. Curtis Klaassen, John B. Watkins III. Casarett & Doull’s essentials of Toxicology, 3rd Edition,
2015.
Reference books and articles:
1. Lynn Wecker, Lynn Crespo, Genorge Dunaway, Carl Faingold and Stephanie Watts. Brody’s
Human Plarmacology, Elsevier 5th Edition 2010.
2. Stan Bardal, Jason Waechter, and Doug Martin. Applied Pharmacology. Elsevier 2011.
3. Ernest Hodgson. A Textbook of Modern Toxicology, 4th Edition 2011.
4. Laurence Brunton, Bruce A. Chabner, Bjorn Knollman. Goodman and Gilman’s the
Pharmacological Basis of Therapeutics, 12th Edtion, McGraw Hill Education (2011)
5. R.S. Satoskar, [Link], Nirmala N. Rege, R.R. Satoskar. Pharmacology and
Pharmacotherapeutics 20th Revised Edition, Popular Prakashan (P) Ltd (2014)
6. Bertram G. Katzung, Susan B. Masters, Anthony J. Trevor, Basic and Clinical pharmacology
12th edition, McGraw-Hill Medical (2015) 1. Karen E. Stine. Thomas M. Brown. Principles of
Toxicology. 3rd Edition CRC Press (2015)
Recommended by Board of Studies 03.08.2017
Approved by Academic Council No.46 24.08.2017

[Link]. Biotechnology Page 70

Course code Course title L T P J C
BST6006 Medical Informatics 2 0 0 4 3
Pre-requisite Syllabus version
v.1.1
Course Objectives:
[Link] understanding of current issues and developments in the medical field, provide basic
skills for managing information
[Link] identify opportunities for the application of informatics principles to medical health research
and practice
3. Distinguish the various types of health care information including data, source, knowledge and
importance of technology and standards.
Expected Course Outcome:
1. Interpret network systems and their application in medical laboratories;
2. Identify the issues surrounding the security of digital information especially as it relates to
patient healthcare information;
3. Examine the application of image analysis software and expert systems/decision support
systems in the healthcare and medical research environment;
4. Perform basic software functions using word processing, spread sheet, database, presentation
and image analysis programs, and
5. Demonstrate the need for standards in the formatting, distribution and storage of medical
information.

Student Learning Outcomes (SLO): 2 ,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Overview of Medical Informatics 4 hours
The Goals of Medical Informatics, Why computers in healthcare? Systems Design Considerations
for the Clinical User.

Module 2 Electronic Health Records: 4 hours

Integrated Practice Management Systems, The Paper-based Medical Record, Current Use of
HER in Healthcare Settings, Vist A (VA EHR System)

Module:3 Clinical support Systems: 4 hours

Hospital Information Systems, Clinical Information Systems, Laboratory Information Systems,
Pharmacy Information Systems.

Module:4 Introduction to Medical Networks: 4 hours

Introduction to Medical Network Design & Development and its applications.

Module:5 Emergence of Medical Informatics: 4 hours

[Link]. Biotechnology Page 71

Medical informatics as a Discipline, Predominant Architectures and Practices.

Module:6 Informatics Issues in Virtual 4 hours

Healthcare, Telemedicine:
e-Health, Virtual Healthcare Delivery System, Issues in Telemedicine: Real-time, Store-
and- forward

Module:7 Medical Informatics, Clinical 4 hours

Decision Making and recent technologies
Measuring Quality and Outcomes, Standards and Quality Improvement and Evidence-Based
Medicine. Integrating the personal health record with mobile technology, implantable chips,
personalized medicine, genome based medical care.

Module:8 Contemporary issues: 2 hours

Industry expert lectures on contemporary issues

Total Lecture hours: 30 hours

Text Book(s)
[Link], [Link]; Musen, M.A. Handbook of Medical Informatics 1st ed. 2014.

2. Computers In Medicine, Tata McGraw Hill, 2015.

3. Davidson, P., Best Practice Series: Healthcare Information Systems, Auerbach Publications,
2015
4. Medical Informatics. A Primer: Mohan Bansal, Tata McGraw Hill, 2013
5. Slack, Cyber medicine: How Computing Empowers Doctors and Patients for Better Health
Care, Jossey-Bass, Revised 2011
6. Ellis, Technology and the Future of Health Care, Preparing for the Next 30 Years, Jossey-
Bass, 2016
Reference Books
1. Bryan Bergeron, Bioinformatics Computing (2012), a complete guide to Bioinformatics for
Molecular biologists and life scientist.
2. Medical Informatics. A Primer: Mohan Bansal, Tata McGraw Hill, 2013

[Link]. Biotechnology Page 72

 Mode of Evaluation: Continuous assessment and Final Assessment test

Recommended by Board of Studies 03-08-2017

Approved by Academic Council No.46 Date 24-08-17

[Link]. Biotechnology Page 73

Course code Course title L T P J C
BST6007 Nutraceuticals 2 0 0 4 3
Pre-requisite Nil Syllabus version
v.1.1
Course Objectives:
1. Build knowledge on the function of nutraceutical compounds to manage various diseases
2. Deduct the marketing potential of nutraceutical compounds related to health management
3. Identify various types of nutraceuticals and molecular mechanism behind the choice of a
specific nutraceutical compound.
Expected Course Outcome:
1. Understand the functional aspect of nutraceutical compounds
2. Extend the importance of nutraceuticals in relation to health
3. Discuss the various processing methods of nutraceuticals
4. Select various sources of nutraceuticals from plant, animal, microbes, and marine origin
5. Predict the diverse nutraceutical compounds involved in disease prevention
6. Decide the safety issues, regulatory policies, health claims, and clinical trials in using
nutraceuticals

Student Learning Outcomes (SLO): 2 ,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Introduction to nutraceuticals and functional 3 hours
foods:
Definition, concept of nutraceuticals; classification of nutraceuticals, dietary supplements,
fortified foods, functional foods; scope involved in the industry- Indian and global scenario.

Module 2 Importance of nutraceuticals: 4 hours

Food pyramid, nutritional assessment, recommended dietary intake, glycemic index, basal
metabolic rate, nutraceuticals in fruits, vegetables and grains with health benefits, nutraceuticals in
relation to sports and exercise. Emerging concepts in nutraceuticals: Life style changes -
Nutraceutical transition and its implications. Enhancing quality and bioavailability of
nutraceuticals. Nutrigenomics.
Module:3 Extraction, analysis, physiology, processing of 4 hours
nutraceuticals:
Nutraceutical extraction and isolation; nutraceutical analysis; absorption, disposition, metabolism,
and elimination of nutraceuticals.

Module:4 Nutraceuticals of plant and animal origin: 6 hours

Phytochemicals as nutraceuticals- sources and applications in preventive medicine; animal
metabolites sources and applications in preventive medicine; protein and peptide‐ based
nutraceuticals, lipid‐ based nutraceuticals

[Link]. Biotechnology Page 74

Module:5 Microbial and marine nutraceuticals: 4 hours
Concept, applications of prebiotics and probiotics as nutraceutical agents, microbial nutraceuticals
and their applications, marine nutraceuticals and their applications.

Module:6 Nutraceuticals in disease prevention: 4 hours

Nutraceuticals for- cardiovascular health, HIV and cancer risk reduction, bone and joint health,
diabetes, hypertension, hypercholesterolemia, immune system, oxidative stress, cognitive
function, anti-ageing, maternal and infant health, gut health, reproductive health.

Module:7 Marketing, regulation, health claims and 3 hours

clinical trials and Emerging concepts in
nutraceuticals
Assessment of safety and efficacy of functional foods and ingredients, regulatory issues and health
claims, use of animal models and pre-clinical and clinical trials involved. Life style changes –
Nutraceutical transition and its implications. Enhancing quality and bioavailability of
nutraceuticals. Nutrigenomics.

Module:8 Contemporary Issues: 2 hours

Industry expert lectures on contemporary issues

Total Lecture hours: 30 hours

Text Book(s)
1. Debasis Bagchi, Harry G. Preuss, Anand Swaroop. Nutraceuticals and Functional Foods in
Human Health and Disease Prevention. 2015. CRC Press.

Reference Books
1. Yoshinori Mine, Eunice Li-Chan, and Bo Jiang. Bioactive Proteins and Peptides as
Functional Foods and Nutraceuticals. 2010. Blackwell Publishing Ltd.

Mode of Evaluation:
Continuous Assessment Tests 1, 2; Quiz 1, 2; Assignment, Term End Examination
Recommended by Board of Studies 03-08-2017
Approved by Academic Council No. 46 Date 24-08-2017

[Link]. Biotechnology Page 75

Course code Course title L T P J C
BST6008 Marine Biotechnology 3 0 0 0 3

Pre-requisite Nil Syllabus version

v. 1
Course Objectives:
1. Explain the scope and challenges in the field of marine biotechnology.
[Link] better knowledge about marine ecosystems, biodiversity & taxonomy, tools and
techniques used and role of marine organisms in biogeochemical cycles.
3. Realize the biotechnological importance and to explore the potential of marine organisms for
human betterment.
4. Develop the students’ skills to take up employment, to pursue research as well as to become an
entrepreneur in marine biotechnology field.
Expected Course Outcome:
1. Develop knowledge about the importance, opportunities and challenges in the field of marine
biotechnology
2. Compare about the various marine ecosystems, their characteristics and biodiversity
3. Implementing various tools and techniques used for sample collection, isolation of micro and
macro organisms and to study their taxonomy.
4. Analyze the importance and role of marine organisms in biogeochemical cycles and global
climate change
5. Discuss and elaborate about various marine pathogenic microbes and their transmission to land
6. Retrieving knowledge on process of drug discovery from marine organisms and various assays
and techniques related to it
7. Utilize marine organisms for food, fuel, agriculture, environment, cosmetics, industrial
feedstock etc.
8. Demonstrate the various techniques and tools necessary for studying marine microbial diversity
and its applications
Student Learning Outcomes (SLO): 2,11
2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Scope and Challenges in marine 8 hours
biotechnology:
Global and Indian scenario; Demand for marine bio products; market value; marine bio-product
based industries; marine bio-economy; Marine socio-economics; Entrepreneurship; International
and Indian policies; Marine biotechnology parks in various states; R&D institutions, centres and
consultation services.

Module:2 Marine Ecology: 5 hours

Benthic and Pelagic Zone; Photic, dysphotic and aphotic zones- importance and their significance.
Biological divisions of the sea- estuaries and backwaters, lagoons, mangroves, coastal waters,

[Link]. Biotechnology Page 76

inshore, offshore, deep sea/oceanic zone.

Module:3 Biological Resources and taxonomy: 5 hours

Sampling, cultivation and taxonomy of organisms. Metagenomics. Flora, Fauna, Bacteria, fungi,
algae and archaea. Extremophilic microorganisms; Fisheries and other aquatic potential.

Module:4 Marine Biogeochemical cycles: 5 hours

Role of marine organisms in carbon, nitrogen, phosphorous and sulphur cycles

Module:5 Marine microbial pathogens: 5 hours

Microbial pathogens in marine environment - diversity, sources and detection of pathogens in
recreational water, impact of harmful algal blooms, microbial pathogens of seafood.

Module:6 Marine Pharmacology: marine products: 6 hours

Marine derived drugs in preclinical and clinical trials- FDA and EMEA approved marine derived
drugs, their use and mode of action. Screening of drugs High-throughput Screening Assays (HTS).
Bioassays- Enzyme assays, cytotoxicity assay; antimicrobial assay; DNA laddering assay;
Apoptosis assays.

Module:7 Marine Bioprospecting: 6 hours

Marine organisms for Biofuels and bioenergy, Bioremediation, Biofouling, Biosurfactants. Marine
natural products as cosmetics-cosmeceuticals, algotherapy; Thalassotherapy; Enzymes; food,
supplement, nutrition and energy drinks. Marine algae as fish feed, manure and fertilizers.

Module:8 Recent Developments in marine and Aquatic 5 hours

Biotechnology
Total Lecture hours: 30 hours

Text Book(s)
1. Frontiers in Marine Biotechnology [Hardcover] Peter Proksch and Werner E.G. Müller (Eds.)
Taylor & Francis; 1 edition (2006)
Reference Books
1. Aquaculture Microbiology and Biotechnology, Volume Two. Didier Montet and, Ramesh C.
Ray (Eds.) Science Publishers; 1 edition (2011).
2. Introduction to Marine Biology. George Karleskint, Richard Turner, and James Small (Eds.)
Brooks Cole; 3 edition (2009).
3. Bioactive Marine Natural Products [Paperback]. Dewan S. Bhakuni and, D.S. Rawat (Eds.))
Springer; Softcover, (2010)
4. John Paul, Marine Microbiology, Elsevier. (1999)
5. Munn and Munn,. Marine Microbiology: Ecology and Applications. BIOS, Scientific
Publisher, (1996)
6. Rheinheimer, G., Aquatic Microbiology-an Ecological Approach, Blackwell Scientific
Publications (1980)
Mode of Evaluation: Continuous assessment and Final Assessment test

[Link]. Biotechnology Page 77

Recommended by Board of Studies 03-08-2017
Approved by Academic Council No. 46 Date 24-08-2017

[Link]. Biotechnology Page 78

 Course code Course title L T P J C
BST6009 Nanobiotechnology 2 0 0 4 3

Pre-requisite Nil Syllabus

versionv. 1.1
Course Objectives
1. To motivate students grasp the basics of nanotechnology.
2. Get exposed to potential applications of nanobiotechnology
3. Ability to assimilate existing and new concepts, methodologies and research results and apply them in an
academic or industrial research environment.
4. Ability to reflect on ethical and scientific problems related to this field.

Expected Course Outcome:

1. To enable them grasp basic concepts and theories of the subject
2. Implement the application of analytical techniques in examining nanostructures/ particles
3. Outline and tell the scope of bio macromolecules in nanotechnology
4. To enable students appreciate the potential of nano biotechnology in consumer applications and diagnostics
5. To develop necessary foundation for training in research
6. Formulate risk assessment strategies in usage of nanostructures/ particles in various applications.

Student Learning Outcomes (SLO): 2 ,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Nanomaterials -biology interface: 4 hours
Advances, development, timelines and overview; nanomaterials in biotechnology: Carbon Nano
Tubes (CNTs), Quantum Dots (QDs), metallic nanoparticles.

Module:2 Bio macro molecules in nano biology: 5 hours

Self-assembly of lipids, proteins; static and dynamic self-assembly; DNA based nanostructures/
devices, size distribution.

Module:3 Biogenic and biomimetic nanoparticles: 4 hours

Plant, microbial routes; types, synthetic procedures; magnetosomes; ferritins.

Module:4 Characterization of biologically relevant 3 hours

nanoparticles and structures:

Zeta potential; Dynamic light scattering; UV-visible and fluorescence spectroscopy, SERS. FTIR; XRD,
EDAX; Microscopy: SEM, TEM, AFM; SPR based imaging; nano secondary ion mass spectrometry
(NanoSIMS).

[Link]. Biotechnology Page 79

Module:5 Nanotechnology in food and agriculture: 4 hours
Food quality monitoring; adulteration; food packaging; food grade nanomaterials and safety assessment.

Module:6 Nanotechnology in environment: 4 hours

Environmental sensors for monitoring pollutants. Nanomaterials based remediation approaches.

Module:7 Nanotechnology in health care applications 4 hours

and toxicity effects of nanomaterials:
Tissue engineering, targeted drug delivery- nano-diagnostics; evaluation of nanoparticles (NPs) in animal
models, cellular uptake.

Module:8 Nanomaterials in consumer products: 3 hours

Cosmetics, toothpaste; environmental release. Cellular interaction and fate of nanoparticles in environment.

“ J” COMPONENT:

Total Lecture hours: 30 hours

Text Book(s)
1. The Nanobiotechnology Handbook, YubingXie,2012, CRC Press, Taylor Francis group

2. Nanobiotechnology: Concepts, applications and perspectives, eds. CM Niemeyer,

CAMirkin, 2005, Wiley-VCH Verlag GmbH & Co., KgaA, Weiheim.

3. Nanobiotechnology II: More Concepts and Applications, eds. CA Mirkin, CM Niemeyer,

2007, Wiley-VCH Verlag GmbH & Co., KgaA, Weiheim.

Reference Books
1. Nanotechnology: An introduction to nanostructuring techniques, eds. M Kohler,
WFritzsche, Wiley-VCH Verlag GmbH & Co. KgaA, Weiheim.

2. Relevant articles from Web and recent review articles from peer reviewed scientific journal.

Mode of Evaluation: Continuous assessment and Final Assessment test

Recommended by Board of Studies 03.08.2017

Approved by Academic Council No.46 Date 24.08.2017

[Link]. Biotechnology Page 80

Course code Course title L T P J C
BST6010 Applied Enzyme Technology 3 0 2 0 4
Pre-requisite Nil Syllabus
v. 1.1
version

Course Objectives:
1. To learn kinetics of enzymatic reactions and to understand its catalysis process
2. To analyse the effects of parameters affecting enzyme kinetics and formulate methods to evaluate
enzyme kinetics in homogeneous and heterogeneous systems;
3. To know the technologies of production of industrial enzymes and understand medically important
enzymes.

Expected Course Outcome:

1. Demonstrate the understand of the enzyme and its catalysis process
2. Students would be confident in preparing Immobilizing enzymes and understand its kinetics behavior
3. Students are able to say about production of industrial enzymes and understand its application in
Industry and other fields.
4. Students can interpret about different kinds of medically important enzymes and how are they
diagnosed in the clinical settings.

Student Learning Outcomes (SLO): 2 ,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Introduction and history of Enzymes: 5 hours
Classification of enzyme;Types of enzymes- Constitutive enzyme, induced enzymes,
Intracellular and Extracellular enzymes
Module:2 Enzyme kinetics: 7 hours
Factors affecting rates of enzyme catalyzed reactions, concept of Brigs Haldene Modification
on M-M
Module:3 Classification and kinetics of multi-substrate reactions: 6 hours
Enzyme Inhibition and
reactions:drug discovery. .
Module:4 Purification and Characterization: 6 hours
Production and purification of crude enzyme extracts from plant, animal and microbial sources;
methods of characterization of enzymes
Module:5 Enzyme Immobilization: 6 hours

[Link]. Biotechnology Page 81

Physical and chemical techniques for enzyme immobilization with examples. Advantages
and disadvantages of different immobilization techniques. Effect of solute partition and diffusion
on the kinetics of immobilized enzymes.
Module:6 Applications of immobilized enzymes: 5 hours
Enzyme reactors - Continuous flow reactors, Packed bed reactors, Continuous flow stirred tank
reactors, Fluidized bed reactors
Module:7 Enzymes in medicine: 6 hours

Enzymes in diagnosis - GOD, Urease, LDH, ALP, CK, SGPT & OT. Enzyme in therapy- α -
amylase, bromalain, rennin, papain, catalase, streptokinase and urokinase. Application of enzymes
in food, pharmaceutical and other industries; Enzymes for analytical and diagnostic applications
Module:8 Enzyme Biosensors 5 hours

Types of Biosensors; design of enzymeelectrodes and their application as biosensors in

industry, healthcare and environment. Novel enzymes from natural resources, Modified
enzymes- enzyme engineering, semisynthetic enzymes, abzymes and synzymes
Total Lecture hours: 30 hours

Text Book(s)
1. Enzymes by Palmer Horwood Publishing Series. 2001

2. Fundamentals of Enzymology by Price and Stevens Oxford University Press. 2002

Reference Books
1 Biocatalysts and enzyme technology, Klaus Buchholz, Volker Kasche, Uwe Theo Bornscheuer,
Published by Wiley-VCH, 2005.
2. Wiseman, A: Handbook of Enzyme Biotechnology, 3rd Edition, Ellis Horwood Publication,2010
3. Enzymes: Biochemistry, Biotechnology, Clinical Chemistry by Trevor Palmer Horwood
Publishing House, Chichester, England, 2001.
4. Practical enzymology. By Hans Bisswanger. Wiley Publication. 2nd Edition, 2011

List of Challenging Experiments (Indicative)

1. .Extraction of acid phosphatase from sweet
2 To study time course of the reaction catalyzed by alkaline phosphatase (EC [Link])
3 To examine the effect of enzyme concentration on the rate of an enzyme catalyzed reaction

[Link]. Biotechnology Page 82

4 To examine the effect of pH on activity of alkaline phosphatase
5 To study the effect of substrate concentration on activity of ALP and determine the Km and
Vmax of the reaction
6 To determine temperature optima for Alkaline phosphatase
7 The hydrolysis of sucrose by yeast β-Fructofuranosidase
8 Estimation of lipase activity
9 Determination of LDH in liver tissues
10 Determination of SOD and Catalase in hemolysate
Total Laboratory Hours:15
Mode of Evaluation: Continuous assessment and Final Assessment test.
Recommended by Board of Studies 03.08.2017
Approved by Academic No. 46 Date 24-08-2017
Council

[Link]. Biotechnology Page 83

BST6011 Course title L T P J C
Metabolic Engineering
Pre-requisite NIL Syllabus version
v.1.1
Course Objectives:
1. To provide a basic knowledge about strategic manipulation of metabolism
2. To provide quantitative perspective of metabolic regulations and developing metabolic models
3. To demonstrate metabolic network construction and reconstruction
Expected Course Outcome:
1. Apply knowledge of mathematics, science, and engineering
2. Integrate modern biology with engineering principles
3. Identify, formulate, and solve biochemical engineering problems
4. Analyze flux to identify nodal control
5. Model enzyme kinetics and metabolic fluxes along with control
6. Design metabolic models to represent metabolic networks in single cells and at the
organ level

Student Learning Outcomes (SLO): 2 , 11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Basics of metabolic engineering 6 hours
Cellular metabolism, order and molecularity of the reactions, stoichiometry of cellular reactions,
reaction rates, dynamic mass balances, yield coefficients

Module 2 Metabolic pathway analysis and regulation 8 hours

Metabolic pathways databases, Overview of enzyme activity and concentration; global control
regulation; Limiting accumulation of end-products

Module:3 Basics of metabolic flux analysis 7 hours

Concept of Nodal points, Linear and Branched pathways, Determined, over determined and
undetermined systems; sensitivity analysis, Fiatflux software for MFA

Module:4 Methods for Metabolic Flux and Control 6 hours

analysis
Direct flux determination, enumeration of metabolite isotopomers (NMR and MS), carbon
metabolite balances, Flux control coefficients

Module:5 Methods for Metabolic control analysis 6 hours

Determination of flux control coefficients, concentration control coefficients

[Link]. Biotechnology Page 84

Module:6 Metabolic design: 5 hours
Synthetic Biology, Design of Genetic circuits, Recent developments in Metabolic design,
Constraint based Genome scale models

Module:7 Metabolic engineering in Practice: 5 hours

Randomized and targeted strain development strategies, Applications of Metabolic Control
Analysis

Module:8 Contemporary issues: 2 hours

Lecture by Industrial Expert

Total Lecture hours: 45 hours

Text Book(s)
1. George Stephanopoulos, Aristos A Aristidou, Jens Nielsen (2005) Metabolic Engineering -
Principles and Methodologies. Academic Press Inc.

2. H. Sahm, C. Wandrey, Metabolic Engineering, 2014, Springer Berlin Heidelberg.

Reference Books
1. S. Sen, L. Datta and S. Mitra (2018) Machine Learning and IoT: A Biological Perspective,
CRC Press, Taylor and Francis Group

2. Michael E Wall, (2012) Quantitative Biology: From Molecular to Cellular Systems, Chapman
& Hall.
Arul Jayaraman, Juergen Hahn (2009) Methods in Bioengineering: Systems Analysis of
3.
Biological Networks, Artech House Publishers.
Mode of Evaluation: Continuous assessment (Digital assignments, Online quiz,
Examination)
Recommended by Board of Studies 03.08.2017
Approved by Academic Council No.46 Date 24.08.2017

[Link]. Biotechnology Page 85

 Course code Course title L T P J C
BST6012 Plant Biotechnology 2 0 2 4 4

Pre-requisite Nil Syllabus version

v. 1.1
Course Objectives:
1. To equip students with knowledge on basic tools and principles of plant biotechnology for
crops resources research and development
2. To introduce students to practical applications of plant biotechnology in agriculture
3. Understanding of biotechnological processes has also applicative value in pharmaceutical and
food industry, in agriculture and in ecology
Expected Course Outcome:
1. Execute the basics of the physiological and molecular processes that occur during plant
growth
2. Students will acquire a comprehensive knowledge on plant tissue culture, methods of gene
identification, engineering of the identified genes and genetic manipulation of plants and in the
field of Plant biotechnology.
3. Learn the various components involved in developing transgenic plants
4. Compare and apply molecular marker technology in plant breeding
5. In addition, this course will provide an overview of plant biotechnology in applications such
as producing drugs and vaccines for pharmaceutical industry and creating GM crops for better
human nutrition.
6. Practice tissue culture techniques and get employed in a plant biotechnology based industry.

Student Learning Outcomes (SLO): 2 ,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Introduction to plant biotechnology: 2 hours
Basic concepts and history of biotechnology; need, scope, outline of plant biotechnology.

Module:2 Plant tissue culture: 2 hours

Media and culture conditions- basal media, growth regulators; micro propagation; culture types and
their uses- callus and somatic embryo culture, cell suspension cultures, pollen/anther culture, protoplast
culture, meristem culture; regeneration methods of plants in culture-organogenesis, somatic
embryogenesis; hairy root cultures, artificial seeds.

Module:3 Production of transgenic plants: 4 hours

Plant genetic transformation, modes of gene delivery in plants- particle bombardment,
electroporation, microinjection, Agrobacterium mediated gene transfer, Ti and Ri plasmids, screening
and selection of transformants, marker free transgenics, Bt crops, golden rice.

[Link]. Biotechnology Page 86

 Module:4 Molecular markers and mapping techniques 5 hours
in plant improvement:

RFLP maps, RAPD markers, STS, microsatellite, SCAR, SSCP, QTL mapping, high throughput
genotyping, synteny mapping, plant DNA barcoding, gene pyramiding, RNAi, CRISPR-Cas and
microRNA systems in plant biotechnology.

Module:5 Transgenics in crop improvement: 5 hours

Production of high yielding varieties, resistance to herbicides, resistance to pests and diseases,
manipulating male sterility, tolerance to abiotic stresses, manipulating food quality- prolonging shelf life,
improved nutritional quality.

Module:6 Applications of transgenic plants in industry: 5 hours

Production of plant secondary metabolites, metabolic engineering, plants as factories for production of
industrial enzymes, biodegradable plastics, biopharming and nutraceuticals, edible vaccines,
plantibodies, peptide expression in plants, biofuels
Module:7 Impact of plant biotechnology: 5 hours
Risk factors and regulations related to genetically modified crops (GMC), Intellectual property rights of
GM crops, Transgenic plants – International and National status, biohazards of rDNA technology.

Module:8 Contemporary issues: 2 hours

Industry expert lectures on contemporary issues
Total Lecture hours: 30 hours

Text Book(s)
1. Roberta Smith. Plant Tissue Culture: Techniques and Experiments. Academic Press, 2012
2. Singh B.D. Plant Biotechnology, Kalyani Publishers, 2014.
Reference Books
1. Kole, C., Michler, C., Abbott, A.G., Hall, T.C. (Eds.) Transgenic Crop Plants: Volume 1:
Principles and Development. Springer. 2010.
2. Kole, C., Michler, C., Abbott, A.G., Hall, T.C. (Eds.) Transgenic Crop Plants: Volume 2:
Utilization and Biosafety. Springer. 2010.

List of Challenging Experiments (Indicative)

1. Study on the design and structure of a plant tissue culture laboratory and 2 hours
greenhouse
2. Aseptic techniques - wet sterilization, filter sterilization, irradiation, 2 hours
chemical sterilization and laminar airflow chamber
3. Preparation of stock solutions of basal medium, organic supplements and 2 hours
plant growth regulators; preparation of plant tissue culture media

[Link]. Biotechnology Page 87

4. Surface sterilization of explants, inoculation and micro propagation of plants 3 hours
5. Zygotic embryo culture, leaf bit, root bit, shoot tip, nodal and microspore 3 hours
culture
6. Sub culturing and development of friable calli 2 hours
7. Preparing a suspension culture from friable calli and plotting the growth 3 hours
curve

8. Protoplast isolation and fusion 3 hours

9. Direct/indirect organogenesis; Shooting and rooting 3 hours
10. Isolation of plant genomic DNA 3 hours
11. Co-culturing of explant and callus; floral dip with Agrobacterium 2 hours
tumefaciens; studying carrot tissue proliferation on transformation with T
DNA; screening of transformed tissue
12. Hardening techniques; growing in coco peat trays; secondary hardening 2 hours

Total Laboratory Hours 30 hours

Mode of Evaluation: Continuous assessment (Digital assignments, Online quiz, Examination)

Recommended by Board of Studies 03.08.2014

Approved by Academic Council No.46 Date 24.08.2014

[Link]. Biotechnology Page 88

 Course code Course title L T P J C
BST6013 Bioremediation 2 0 0 4 3

Pre-requisite Nil Syllabus version

v. 1.1
Course Objectives:
1. Relate the various aspects of pollution sources and microbial resistance to understand
degradation pathways
2. Summarize the microbial and non-microbial degradation processes
3. Build knowledge on the applied aspects of bioremediation
Expected Course Outcome:
1. Defining the sources of pollution and EM technology.
2. Put the the knowledge on microbial resistance towards toxic compounds and degradation
Relating
pathway.
3. Selecting the various remediation technologies for the bioremoval of toxic compounds..
4. Analyzing the knowledge in the fungal and algal biodegradation.
5. Inferring the importance of plant microbe interaction in bioremediation through metabolomics
and importance of GMO’s and bioreactors in bioremediation
6. Designing a novel study with the knowledge and nuances gained from the above modules
Student Learning Outcomes (SLO): 2,11
2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Basics and terminologies in bioremediation: 4 hours

Sources of pollution, Physico-chemical parameters of the pollutants, Screening for effective

microbe for the bioremoval of toxic compounds, Qualitative and Quantitative analysis of the toxic
compounds from polluted site

Module:2 Metabolism of Biodegradation: 6 hours

Microbial and plant resistance towards toxic compounds, metal microbe interactions, Detection of
candidate genes and enzymes involved in the process of degradation – Aromatic and aliphatic
compounds, Application of KEGG pathway in bioremediation

Module:3 Bioremediation: 6 hours

Bioremoval of toxic compounds like heavy metals, pesticides, hydrocarbons and other xenobiotic
compounds, In-situ – Bioaugmentation, Bioventing and others technologies, Ex-situ – solid waste
management (Landfarming, composting and Biopiles).

Module:4 Non-bacterial Biodegradation: 3 hours

Fungal Biodegradation and Phycodegradation, Biodegradation in biofuel production, Co 2
Sequestration

[Link]. Biotechnology Page 89

 Module:5 Plant microbe interactions in biodegradation: 3 hours
Phyto remediation and its types, rhizoremediation strategy and processes, case study in the
removal of heavy metals and other toxic compounds (Chernobyl accident) onsite
Module:6 Bioreactors and Genetically Modified 3 hours
Organisms (GMO):
Aerobic and anoxic type bioreactor for biodegradation - solid, liquid and air (slurry, batch
and continuous processes), Application of GMO’s in Bioremediation

Module:7 Metabolomics studies: 3 hours

Superbugs as super savers, engineered enzymes, products and biosensors involved in
biodegradation, Metabolomics in bioremediation, Recent advances in bioremediation.

Module:8 Contemporary issues: 2 hours

Total Lecture hours: 30 hours

Text Book(s)
1. Ralph Mitchell and Ji-Dong Gu. 2010. Environmental Microbiology, 2nd edition, Wiley and
Blackwell, Inc. .
Reference Books
1. Ralph Mitchell and Ji-Dong Gu. 2010. Environmental Microbiology, 2nd edition, Wiley and
Blackwell, Inc.
2. M. N. V. Prasad, 2016. Bioremediation and Bioeconomy, Elsevier.

Mode of Evaluation :Continuous assessment (Digital assignments, Online quiz, Examination)

Recommended by Board of Studies 03-08-2017

Approved by Academic Council No. 46 Date 24-08-2017

[Link]. Biotechnology Page 90

 Course code Course title L T P J C
BST6014 Genomics and Proteomics 3 0 0 0 3
Pre-requisite Nil Syllabus version
v. 1.1
Course Objectives:
This course aims to provide you with the knowledge and research skills associated with
functional genomics and proteomics.
Expected Course Outcome:
1. At the end of the course student should be able to discuss about the Genome biology.
2. The student would be able to apply the tools available in the open source to the specific research
problems and projects
3. The students can interpret the data obtained from high through studies.
4. Communicate on the recent developments in the genomics and proteomics, and its application in
human disease biology.
5. Discuss about the emerging areas of biology that use the basics of Genomics and Proteomics.
[Link] effectively in interdisciplinary teams
Student Learning Outcomes (SLO): 2 ,11
2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Genome overview: 7 hours
Structure and organization of genomes, Genome size, Sequence complexity, Modern Genome
Sequencing, Genome mapping, Human Genome project, Chromosome landmarks, Genetic
variations, Physical maps

Module:2 Genomics: 6 hours

Gene identification and annotation, Functional and comparative genomics, DNA and disease
association, Epigenomics

Module:3 Proteome overview: 7 hours

Definition, Worm proteome, Fly proteome, Strategies for protein identification, Protein
sequencing, Peptide Mass Fingerprinting

Module:4 Proteomics: 5 hours

Protein Engineering, Protein Chip technology, Cancer Proteomics, Antibody microarrays, Protein
modifications

Module:5 Bridging Genomics and Proteomics: 5 hours

Domain architecture, Sequence-structure mapping, Protein folding, forces and interactions,
Regulomes, Stimulomes and Phenome, Secretome

Module:6 Applications I: 5 hours

Metagenomics, Toxicogenomics, Gene therapy, Glycobiology

[Link]. Biotechnology Page 91

 Module:7 Applications II: 5 hours
Proteomics in plant genetics and breeding, Diagnostic Proteomics, New approaches to therapy,
Future of the field

Module:8 Contemporary issues: 5 hours

Total Lecture hours: 45 hours

Text Book(s)
1. S.B. Primrose and R.M. Twyman, [Link] of Genome analysis and Genomics. 7th
edition, Blackwell publishing.

2. Principles of Proteomics. R. Tymann, 2nd edition, Garland Science, 2013

Reference Books
1. Cancer Genomics and Proteomics: Methods and Protocols, Narendra Wajapeyee, 2014,
Springer New York.
Recommended by Board of Studies 03-08-2017
Approved by Academic Council No. 46 Date 24-08-2017

[Link]. Biotechnology Page 92

Course code Course title L T P J C
BST6015 Signal Transduction 2 0 0 0 2
Pre-requisite Syllabus version
v.1.1
Course Objectives:
1. Explain the process of disease progression in terms of cellular signalling
2. Discuss the aetiology, available diagnostics, and treatment methodologies for varying disorders
3. Develop the products or processes which are ultimately aimed in interfering and thus treating
the disease cost effectively
4. Distinguish the utility of different analytical techniques that can be used to study the outcome of
the disease
5. Device Omics based methodologies for qualitative and qualitative analysis for the prediction
and application while treatment
Expected Course Outcome:

1. Building the ability to be socially intelligent with good SIQ (Social Intelligence Quotient) and
EQ (Emotional Quotient)
2. Having a clear understanding of the subject related concepts and of contemporary issues
3. Developing problem solving ability both for solving personal and social issues
4. Adopting critical thinking and innovative skills
5. Solving problem by computational thinking (Ability to translate vast data in to abstract
concepts, and to understand database reasoning)
Student Learning Outcomes (SLO): 2 ,11
2. Having a clear understanding of the subject related concepts and of contemporary issues.
[Link] interest in lifelong learning
Module:1 Introduction to Signal Transduction 2 hours
Signals and receptors, Importance and overview of signal transduction

Module 2 General principles and mechanisms 2 hours

Principles and mechanisms of various signaling molecules, second messengers, signaling
networks information flow, computation and decision making

Module:3 Signaling Receptors 3 hours

G-protein and GPCR, receptor tyrosine kinases and activation of Ras, TGFβ, cytokine receptors
and JAK/STAT pathway, calcium signalling.

Module:4 Signaling Pathways 5 hours

MAP Kinase, PI3K-PKB/Akt pathway, mTOR signalingCyclic AMP pathway, Wntsignaling,
Hedgehog signaling, Notch signaling.

Module:5 Signaling processes: 5 hours

Toll-Like receptor signaling, immunoreceptor signaling, signaling by nuclear receptors, Hippo
pathway, signaling pathways that control cell proliferation, signaling pathways that regulate cell
division, signaling in control of cell growth and metabolism.

[Link]. Biotechnology Page 93

Module:6 Signaling Mechanisms: 5 hours
Signaling networks that regulate cell migration, signaling pathways in cell polarity, cell fate and
embryonic patterning, signaling by sensory receptors, synaptic signaling in learning and memory,
signaling in muscle contraction.

Module:7 Signaling in diseases: 5 hours

Cell signaling and stress responses, signaling in innate immunity and inflammation signal
transduction in cancer, signal transduction in diabetes, obesity, cardiovascular system

Module:8 Advanced techniques to visualize the 3 hours

signaling molecules
Cellsignaling assays, imaging techniques, FRET, confocal, flow cytometry, fluorescent tags,
single-molecule tracking

Total Lecture hours: 30 hours

Text Book(s)
1. 1. Signal Transduction: Principles, Pathways, and Processes (2014). Lewis Cantley, Tony
Hunter, Richard Sever, Jeremy Thorner. Cold Spring Harbor Laboratory Press, NY, USA.
Reference Books
1. Signal Transduction. Third Edition (2016). Ijsbrand M. Kramer. Academic Press, USA.

Mode of Evaluation: Use of technology in teaching, lecture by industry experts, Written

examinations, Projects and assignments
Recommended by Board of Studies 03.08.2017
Approved by Academic Council No.46 Date 24.08.2017

[Link]. Biotechnology Page 94

Course code Course title L T P J C
BST6016 Cellular and Molecular Biophysics 3 0 0 4 4
Pre-requisite Nil Syllabus version
v.1.1
Course Objectives:
1. Develop knowledge on various single molecule biophysical techniques
2. Discuss biophysics of the neural system, and radioactivity
3. Explain X-ray crystallography, NMR, and other biophysical techniques and their applications.
Expected Course Outcome:
1. Determine the molecular interactions, thermodynamics, protein folding.
2. Explain the principle and applications of various biophysical methods/techniques.
3. Choose the single molecule biophysical techniques.
4. Discuss various aspects of neuro biophysics.
5. Deduct the different types of radioactivity applied in biological system, and their applications.
6. Evaluate the applied aspects of biophysics.

Student Learning Outcomes (SLO): 2 ,11

2. Having a clear understanding of the subject related concepts and of contemporary issues
[Link] interest in lifelong learning
Module:1 Introduction to Biophysics: 6 hours
Molecular orbitals and covalent bonds, molecular interactions in macromolecular structures,
thermodynamics, protein folding, simulating macromolecular structure-energy minimization,
molecular dynamics.

Module 2 X-ray Crystallography: 7 hours

Symmetries, point groups, space groups, growing crystals of biological molecules, solving
macromolecular structures by X-ray diffraction- structure factor, phase problem, molecular
replacement, Patterson function, multiple isomorphous replacement, anomalous dispersion,
refinement of the structure.
Module:3 Nuclear Magnetic Resonance Spectroscopy: 5 hours
Spin-spin interaction, relaxation and NOE, measuring the spectrum, one-dimensional NMR of
macromolecules, 2D-FT NMR.

Module:4 Principles and applications of other 6 hours

biophysical techniques:
Dynamic light scattering, small angle X-ray scattering (SAXS), Raman scattering, circular
dichroism, isothermal titration calorimetry (ITC), differential scanning calorimetry (DSC), surface
plasmon resonance (SPR)

Module:5 Single molecule biophysics: 8 hours

[Link]. Biotechnology Page 95

Why study single molecules-Applications-Interferometric scattering microscopy (iSCAT),
FIONA- Fluorescence Imaging at One Nanometre Accuracy, TIRFM-Total Internal Reflection
Fluorescence Microscopy, FRET- Forster Resonance Energy Transfer, Optical Trap/Tweezers,
and
Scanning Tunnelling Microscopy (STM).

Module:6 Neuro biophysics: 6 hours

Brain: parts, lobes, functions; Sleep, Human memory- encoding, storage and retrieval, Human
memory- forgetting, artificial neural networks: feed forward nets, recurrent networks,
unsupervised learning, reinforcement learning, back propagation, applications of neural networks.

Module:7 Radiation biophysics: 5 hours

Radiation definition, units; Radioactivity- rate of radioactive decay, measurement of radioactivity,
applications of radio isotopes, biological effects of ionizing radiations, Positron Emission
Tomography (PET), use of radiation in diagnosis and analysis.

Module:8 Contemporary Issues: 2 hours

Industry expert lectures on contemporary issues

Total Lecture hours: 45 hours

Text Book(s)
1. Molecular Biophysics for the Life Sciences (2013). Norma Allewell, Linda Narhi, Ivan
Rayment. Springer Publications.

2. Fundamentals of Biophysics (2014). Andrey Rubin. Wiley Publications.

Reference Books
1. Biophysics (2010). Vasantha Pattabhi, N. Gautham. Alpha Science Intl Ltd, USA

Mode of Evaluation:
Continuous Assessment Tests 1, 2; Quiz 1, 2; Assignment, Term End Examination
Recommended by Board of Studies 03-08-2017
Approved by Academic Council No. 46 Date 24-08-2017

[Link]. Biotechnology Page 96