Experiment No 1
Good Laboratory practices and safety measures
Chemicals Required
Glass ware Quantity
Equipment
Experiment No 2
Study of Microscope
Chemicals Required
Glassware Quantity
Equipment
Microscope 4
Experiment No 3
Preparation of Nutrient agar
Chemical requirement:
1. Powdered nutrient agar (50g)
2. Distilled water (Quantity sufficient)
3. Cotton (I pack)
Glassware Quantity
1. Conical flask 10
2. Aluminum foil 1 pack
3. Sterilized Petri plates 10
Equipment:
1. Autoclave 1
2. Laminar flow hood 1
3. Weighing balance 2
Experiment No 4
Preparation of Nutrient broth
Chemical requirement:
1. Nutrient broth powder (50g)
2. Distilled water (Quantity sufficient)
Glassware Quantity
Conical flask (100ml) 10
Beakers 10
Glass Tubes 10
Falcon tubes 10
Stirrer 10
Equipment:
Autoclave 1
Laminar flow hood 1
Weighing balance 2
Experiment No 5
Enumeration of microorganism
Chemical requirement:
1. Nutrient agar powder (50g)
2. Tap/ pond water sample (10ml)
Glassware Quantity
Sterile petri plates 10
Pipette 5
Marking pen 7
Pipette bulb 7
Mechanical pump 7
Equipment:
Water bath 4
Autoclave 1
Laminar flow hood 1
Colony counter 5
Incubator 1
Experiment No 6
Catalase test
Chemical requirement:
1. Bacterial colony
2. H2O2 (20ml)
Glassware: Quantity
Glass slides 10
Wooden applicator sticks 10
Test tube 10
Equipment:
Laminar flow hood 1
Spirit lamp 5
Experiment No 7
Coagulase test
Chemical requirement:
1. Bacterial sample
2. Distilled water (Quantity sufficient)
3. Blood Plasma
Glassware: Quantity
Glass slides 10
Wax pencil 5
Test tubes 10
Holder 10
Spirit lamp 5
Equipment:
Laminar flow hood 1
Spirit lamp 5
Incubator 1
Experiment No 8
Identification of bacteria by Gram staining
Chemical requirement:
1. Methanol (10ml)
2. Crystal violet dye (10ml)
3. Distilled water (Quantity sufficient)
4. Bacterial colony
5. Safranin (10ml)
6. Iodine (10ml)
Glassware: Quantity
Glass slides 10
Bunsen burner 5
Equipment:
Microscope 4
Laminar flow hood 1
Slide Stand 2
Experiment No 9
Microbiology of air
Chemical requirement:
1. Nutrient agar (50g)
Glassware: Quantity
Flasks 100ml 10
Petri plates 10
Beakers 100ml 10
Inoculating loop 5
Equipment:
Autoclave 1
PH meter 3
Laminar flow hood 1
Incubator 1
Experiment No 10
Microbiology of Water
Chemical requirement:
1. Agar (50g)
2. Water sample
3. Distilled water (Quantity sufficient)
Glassware Quantity
Flasks 100ml 10
Beakers 100ml 10
Petri plates 10
Inoculating loop 5
Equipment:
Autoclave 1
Laminar flow hood 1
Weighing balance 4
Incubator 1
Experiment No 11
Fungal Staining
Chemical requirements
1. Lactic acid (70ml)
2. Phenol (70ml)
3. Glycerol (140ml)
4. Cotton blue/Aniline blue (0.2g)
5. Distilled water (Quantity sufficient)
6. Sabouraud dextrose agar (SDA) (50g)
Glassware Quantity
Dropper 5
Inoculating loop 5
Glass slides 10
Cover slips 10
Beakers 100ml 10
Pipette 5
Graduated cylinder 100ml 10
Equipment:
Autoclave 1
Laminar flow hood 1
Compound light microscope 7
Incubator 1
Experiment No 12
Microscopic test for malaria
Chemical requirement:
1. Cedar wood oil (20ml)
2. Romanowsky stains (10ml)
3. Buffered distilled water (Quantity sufficient)
4. Distilled water (Quantity sufficient)
Glassware Quantity
Glass slides 10
Applicator sticks 10
Cover slips 10
Disposable needles 10
Gloves 14
Pipette 5
Equipment:
Laminar flow hood 1
Compound light microscope 7
