10/27/23, 4:52 PM How to Test Defoamer for Fermentations?

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How to Test Defoamer for

Fermentations?

Defoamer for
fermentations type:
Silicone
Polyether
Fatty alcohol
Mineral oil

Defoamer for fermentations performance: (Cyclic Bubble Test)

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10/27/23, 4:52 PM How to Test Defoamer for Fermentations?

Accurately weigh 200ml of foaming medium

with a 1000ml measuring cylinder, turn on the
air bubble to the specified foam and mention
800ml and record the blank time, then add the
prepared defoamer for fermentations sample,
stir it evenly, then turn on the air bubble and
record the volume V changes with time.

Solid Content Test (instrument: oven, Surface dish)

1. Turn on the oven, set the temperature to 105°C, wash and dry the watch glass
2. Weigh a certain amount of defoamer sample on the watch glass, and record the weight of
the watch glass before and after adding the antifoam as ml and m2
3. Put the watch glass with the sample in the oven, close the oven after 1h, put the watch
glass in the desiccator and cool it, then weigh it as m3;
4. The solid content of the sample is: W=(m3-m1)/(m2-m1)*100%

Viscosity (instrument: mechanical viscometer, constant temperature water bath)

1. Turn on the water bath and set to the desired temperature

2. Wash the viscometer, add a standard amount of defoamer for fermentations sample, turn
on the circulating water, and keep the temperature constant for 15min
3. Calibrate the viscometer, select the appropriate rotor, turn on the viscometer, read and
record

Particle size: (Test D50, D90 and tail peak with a particle size analyzer)

Turn on the power of the dispersing device of the particle size analyzer and the optical system, warm up for about
30 minutes, and then start the computer. Run the laser particle size analyzer program.

Cloud point

1. If the polyether is soluble in water, use distilled water to prepare a% polyether aqueous
solution;
2. If polyether is insoluble in water, first prepare 10% M224 aqueous solution with distilled
water, and then use M224 aqueous solution to prepare% polyether solution.

Specific steps:

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10/27/23, 4:52 PM How to Test Defoamer for Fermentations?

Pour the polyether solution prepared above into a 10ml centrifuge tube and place it in a water
bath for heating. While heating the centrifuge tube, stir the polyether solution up and down with
a thermometer at the same time. After the solution begins to become cloudy, remove After
centrifuging the tube, the temperature is lowered in ice water or tap water. During the cooling
process, the polyether solution is continuously stirred up and down, and the temperature at
which the polyether solution becomes clear is recorded. This temperature is the cloud point of
the polyether.

Hydroxyl value (phthalic anhydride titration method)

Thermal stability (observing the temperature or precipitation of oil bleaching or

precipitation during heating)

Self stability (shaker method, oven method)

If you have any question, Please contact us.    

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