— = Bic onthe
feference
(apace tetera racic ral renee CTnorgani¢)
Himalaya publishing house, Edition -201»_, Pg Noi 1 to 310 _
| Trdian Pharmacopoeia _volume-2 _Edikion-2014
en ae ee istry:
—Pg-No +1999
lex minolo4i 5:
ee re is Science that amakes rise of the cece
Be Oe Sintcley
Lam —cuuithoaity
imoxgam ic _Sustamces 4 drugs Le. Prepac tion,
| Chemical neitune Composition, Staucture influence Ow _
| an _Caganisms_, PLCs ees
Pharxmacopoe fe + ee
[Tt is a book of divections Aan ee fox the.
tk 1S _gemenciily Publishect ty —_§
Thus pharmacopoeia is a legislation ofa
Countasy which Sets Stamdarxcds amd Obligatery GWatlty —
Imadices fox clings cea meltericilS a'Secl to prepare therm —
| @xeparcttion of medicine +
— and Yanious pharmaceutical preparcitions These gegluleifor
13 ine ay ee a
Cinricles DAN ee
aM
ee = z =
1 Oppendices may also include Stomdotd_ni_Apeealia—
—Othen Chemicals , techmiques processes ett, Fetertecl
at Slt pronase
4, Impurities :
| A Compound is Solid to be fmpuse tf ft io hawitacy
2. ©__Vynbilnibteg ee
bee a! Ih Geese. sh
=| Limit tests ane quantitative ov Semi- quamertetive
f ae to _idemtliy cmd _Contgol Smal) quantities
lof Stmpurity orhich axe likely to be present fn the
Pa licirecc, ee Oe
ae een sia: se a
aa of detaining. the Weumeis eee
as _titvattion. ‘DAVE
AIM, a es “
4] Titwerte + é z
=, The _Substernce to be determined 16 termed cS the
—titwate
4. Tad icertoxs a oe
=| Some—cluyilliany Substance erhich is tged to detect
——|-the_emd point of the tftiation 16 tewmedl athe Pndicate
| as
ae pola £
a he Aint cot hich | eee ee sic (oneal eee
feet cise the ead’ asin EEE ee
we
a a Fqurinctence point
Pe onin at csnich the cid 8 goede
| Ccpuivatent cmounts io tecmed a5 equivettence Point
ae eee Peete 20
eS hie ideci| titration SAD ith eee acer omen
—_____| arith the theoxitical end paint Tm _practite honevex
Q Small diffevence Accurs , This difference iS teemed
pS ds titration enor Be
43] Bucette
pee | The “process by cahich Ee Corn Conia ae tee a
| is accunatel determined is Collect Burst,
—__=| A bwuth is qa qua Lucitcel gle. tube (shich a tap ot
——__| One + erate see cence _Noabime ni os
ae fqutid Specially im _tita oa eee DATE
14, Pipette: Oe
ae ee ieee a
_ | 02 amoung Small _enmount of Liquide
AIM
ee Ninticneivis pinetee
=} T4185 a_precise _Jaboadtory - he ene omens
See ici Fey Polonetcice eerie Volume Of ticyuid Dt
———| typically has a single Calibration mark _and_is designed
| fox very accurate VYolime rnecisunements.
——————— eee Ss ee
_——16|_Graaduate pipette: eee
____=| Tt_ is a pipette with hid \lolume im_imctements.,
——__|smaxked__along the _tube._Tt iS 119ecl_to _clcereattely =
| emeciaune amd transfer cr Volume of liqutd fom One
Comtainex tO co there. __ ati ee
BS Vasa
comme roi | Primary peer inellce ose ae
The process _ by _cahich the Ce re of Solution —
AS ACC Lect tel ee Sy ity ee A er
+
| Tt id meagent that _is— extiemely Puce, Stcble hes no
| grater of bydaation amd _had—a_high molecule weight
a 5 € Condon} Standard Se’ Gn ra
| rina hy es
=.) Nosema ality
(A SuUbStamces Ghose active cigent Contents have been
Compaxison __agyuinst_a_ fatmary Stcngared
|_ T+ omely Weg ere eee enecectes cunt eouaivellent
| of Solute present fn One litve of Solution. Tt is ___
epaesentecl by” NA
Teacher's Signaturg = et Lhe: rmolality 2 8 Sotudigns mos ean by tHe —
| mumbex of “mates_of Solute. mT
et 1G) enxecemsecl by Mam, Eset
2 Pl Corrente Weight by Weight Naty)
P= Wiisxiop iiheve pis peccentage of Comstituent —
= Teeauived 11 = Weight of conStitumt ud = Leerght ——
see o_o taken. ee
aa ae
ae. Penton tage Weight by Volume Ct Wily) 2 __
=| Tt_i5_ defined as mass of Soliite des divided vol
Of Solution & oe we
3H, Pexcen tage. “Whthnised List Notume C4-Viy) :
| Tt ig ct measune of the Contentaction aba a Gaiam
| fna Solution Tt iS yepaesented as the yatio of the
Molume of the Solute to the {otal volume of the Solu ton
}rnmiltl ptieal by tC ________ es
os Bel Acid. Gase _Thation: ae eee
| hem the meutyalisation seaction is frvotved ee
| the titacatlon is _tewmed cls _Acid- base ox neutralisation
ee | tivation cate
Mc! Reda» ‘Titiatinn 2
=| Th fnvolves eduction yeaction Tk ts a titaation in _
| eohich the Analyte — And Thaant Seact thyow Qn
| oxidation -tecluctton, xenctton DATE
AIM
Involved Certain organic yeagents Such 45 €thylene =
| Compie% _estth vyercious metal fons amd is thus asec
——_} fox _amalyaia of these metal jong __
ee Be cintatien Tinato
These _fnvolve the formation of the precipitate |
|
Teachers Sipping ene DATE
AIM Mo p of Act C 7
WM oncenticiel Vudvecuioale ack
Veale bs Mol. Wt. 36.5-
ee Ndainchipaic an d_ Contains mot less then 45.0 pextent—
ls cr _amnxe than 440 pexcent Wiis of Hel
| Category, + Pharmacertical aid Oe ae a
Se DESerin tion = SO cn aaa
fee doux a ae _ nea eee
dentification » Shae ie See
__ (A When acide to_potason. peemamgiamade chloxine
ES evinlved uses SAIN
|
By Ti gives the reactinnS of chlowides ££
Se fests =
i |Aasente +: Mix 109 tith hom! of ascites cmd tm of
ee taemous. ‘Chiovide Solution Ast, the yesulling Solution
See | Comolies with the limit test fow Arsenic C1 ppm).
Beas metals :- €vapatate 5.5 - omj-t0_daeyneas-on a
| eatexbath acid 2 ami_of dilute acetic cicid to the
| Residue and adel toater to_amake 25 om1_.The esitting—
— | Sctusfon complies totth the imit test for heen metals
| Method A CS ppm). :
Teachor’s Signature DATE
Int
| Bromide. and Taclide: Dilute Sm cofth 10m! of cater,
add 1m! of Chloxeform And_, caopuise with Constant
1 GShaking __Chloximated lime Solution ,+he Chloxofoxrn
p —_ loyex does mot become hyoron O71 violeb —___
i
ee chinaine = Tb 15 ml add san aoaWaceen
| free water a ml of aio percent Wu Solution of
| potassium iodide cmd _o.5 ml of Staxch Sotttion_cv
| cillers to Stamd fn the ceuck for 2 ominute Any blue
Colour _pyoducecl_chisappears on the cicleition of 0.
of 0.07 M ee eee J ae
| ite + Dilute 4 mt with tom! of water nd cielel
Pee | 005 mt OF oxi Chisaide Golution_clnd—1ml ce
Lo.oi mM fodine .The Coloux_of the todime iS not __
Gmpletely 1S charg ecl___— 05 ee
ee Sipmieteer Pies mg of Sli
bicwrboncte , evaporite to daymess Ona _codltesc ath
‘amd _dissetwe the kesidue in 1 mb of clis tilled esciter
| The %eoul tng — Solution Complies — oith the limit test |
coy oes (C20 ppm)
ere tienc On “evapexation > — _Not 5 awe © n Opes ene
determined on too 4 by eveipasating See onc
| crater bath cmd eee oot tN DATE
—+
AIM
coith 1M Sodium hydaoxide—
4
Solution as Pndicator
5 :
susfng ometheyl! aied =
0.036496 9 of HCl
Pe Teterage = Store ine boppered coniciners of glass
Paani 0 ther ingext material at a temperccitine not
exCeecti ng So"
‘Teacher's Signature DATE
__AM | To _pexfoxm Limit test fos chiogide inthe qfven
Som ple 95 Tndicm phormatanseia-2eai,
Gils te R Ghats!” (phar aa siical Chen eee ieee
| Himalaya _pitblishing house, olitme= T__ Page No:
Requirement: Dilite [4Nos, distilled goo tex_0.2 8 Aging Sal
| Standard Solution of Chloride Test Solittion of Chloride —
fee | Dri of Nesslers cylinder amd lass Yad __
Theos} The Itmit test fox _chioride as been based on the —
[Staple ‘deactton between Silver Nitaate and cautie |
Chioyide to obtain Sflvex Chipxide conic iS —
| tngoluble _im dilute ita’e aetd os
-| The Silver Chipaide pacdicedt. a a
| dilute mitaic acid makes the test Solution turbid the
€ntent of tuibidity depending .ye6n_the Gmount of
Chloride _cind the Same Volume of dilute nftalc acta
ee atised fn the test Solution i ‘
| TP the turbidity fom the Sampte_has been ieace
ae. Standexd turbidity ithe Germple 29ill ass the
emits beet amd wee Sexo
ee cy “Ago Aflac os, agei & os AIM.
|Add _‘lo.mi_of Standard Gol” 11. Add 10 mi of Test Solution —
Peet in messlex Cylinden im nessler cylinder
P| Addo m1 of dil.mitvicacid. |2. Add ip mt of dil mftaic acta,
Make up the volume upto 3. Make up the volume upto |
oA! toith cistillecl oater | 50 ml _esith distitled crater,
coe Hl
Qbsexvatlon :- The Opalesence fn the 4e5+ Solution 1 less, than —
——_| Standaxd Selucion— Lc eee ae
Add_1-ml_of 0.1 nosmal Agia _t. Add devel of 0.1 no%ma) AgNox,
—___f} Stiy_twell till Cpalesence |S Stix tell Lill Opalesence ..
The Opelesence in the +¢9t Solutfon (6 More than
| Standard solution. sae s :
_ The given test Sample does not Comply
test of chiortde as per Imdian pharmacopoeia =2o1ly__
amit
Tin 720s 9 : {ieee
Gexclusign The qiven test Semple Cumali¢s limit test of Chiedlde
ae
| 5 pex Tmdicrn QhosemaCopne tet = 20M gp ge
Teacher's Signature ;_____— DATE
fe lta
AM | Limit test of Sulphate (Gs pecs Dodie plsnecn (tenet
Releaens 0. ce icra eon tn eee aed Cameaganic),—
oe Himatayer a Se
——_—_—_—_——
eT alpetae nate ineec) Sea a eeeee ere soem
Gee | Ethamalic | Salltian. Beium Chloride Solution , Acetic =
| cicid. Distilled cscter. Test Solution -1_, Test Solution-2_—
_Theowy + The Iimit test fox the Sulphate. iS canted out onthe
____| basis of the reaction between Out On the bart
—_____| Chloyide and Goluble Sulphates in presence of dilute -
poe hydaochlowic acid Then, the Comparison _of the twebidt ty:
—_____| producecl by a given crmount of the SubStemce isdone.
—____| With a Standard turbidity Obteimed froma known
Onomount of Sulphcte cond Senme Volume of cflute -
ee oe ea acid have been —acided to both the Solutions
| The bevritim chloride tesi Solution in the TP. has been
| *eplaced by Sarton Sulphate Reagent Gohich is having
| barium Chloxide , Sulphate -Fyee alcohol and a Solution
—__| of potassium Sulphate. Pe lass fin Sulphate has been added
| 40 fnewxease the Sensitivity of the test. The fonfc -_
— Concentrations fn tne Beagent - has been 50 Adjusted
fee | tacit the Soltibility product of bariiim Sulphate gets
letceeded amd the NOcy Small crmount of barctun Sulphate
—__| present in the yengent acts cS 4 Seeciing Agent fee
——_| precipitattan of | baxfumt Sulphate, if Sulphate (S present
|_ tm the Substance tmder 4eSt. Alcoho| heles to _paevent.
—__| Supex- Satunation, anid thus Produces c.mede umf fox a DATE
AIM
____|Opalescence.
——_ iO turbidity that ia less them the Stamdend
The _SubStamce _p fanete
———+|_In_pexforming these _t¢6t6_._ it becomes essential
te folloco the directions cshicnaxe indicated by the—
Be dion Dhowmatnnety
= = fs a
er Bee SOs Bac Hel, Basa, +2017 me
SS ee ——
SE Stan Aasweal unten) eater tran
ta | Add a 25 4 Wy Bevrtum= 1 Add mt o54- Wy Banitrm = |
ce |
| Chloxide olution _____ Chlpxide Solution
2 Add 1.5 ml ethanolic Solution 2 Add 1.5 ml e4thamolic Solution.
Stamd for 1 -mfuute _ 3. Stand foy 1 omfnute _
Add 15 ml Standend Solution t._Add. 15 ml Standand Solution,
fee | | Solution a | Goluition
| Add 0.145 ml, 5M acetic_aci @ [6 Add_0.15.ml._oM Aceticacia |
| Drie upto so-mi with __|6._ Dilute upto _So-ml wit
avo iede tsb Ect led cence: DATE
AIM |
an “Compare Cpatescence. Panduced In tne. test Sottion —
ith thet of Stamdemd Solution zh
Be iy beg a
Chsexvation + The Opdtescence producecl_fn “the_teSt Solution —
Ge 1S lege than that of Atemdeind= Golteiay
Conclusion the qiven test Sample Comply _caith the Glomndand:
| Beainn fox limit test eS ee Imdran =
| A@hasmacopoeia =26 Lt. sa
Qhaexvatlon:- The. pci produced itn Gielen renee
emote them that of 5 tanderd Solute, —_____—
Gonclustdn: ete given Test Sam pl RAR Se rraaltics aulisn.
| the Standand'S fox limit test of a
| Indian _phvenecopacia.- 2G sh a Limit. PP e ako ( mncicin ae ges vais cremate
+
Dolly ae Z en
ae
Refenence. 6x. 8 Chatcoal ,Phavmaceutic Chemistry (Tmoaganic)
|} Himalaya publishing house, slow lume -T_, Py mos __
Reguivemént: Nester Cylinder. Beaker trlass god _ Auber =
_____| Pipette _blub , pipette Ctrmi2. pipette) (40 ml)_,
_______Test- 1 SoluHon _. Test-2 Solution _, Standard Solution
Bee | Acetic acid HS Soliition Distilled” sa ter
Theory The Indicin Pharmacopoeia cmd _thel Sep echoes
| adopted ct Limit test fox heavy metals for aman —
official Substances The +eSt has been designed to
deamonStrate that the Content of metallic _fmpusities
that axe Coloured by brelwogen Sulphide Coulphide fon)
i umdex the Conditions of the 4eSt Should not exceecl
| the heavy metals limit given rimdes_the individuals
LamonogueiphS_._In Substances the Proportion of amy Such
| fampusel Cieavy metals) has been expressed AS the
ee 1 Quon thty Cf lend wequixed te Pacduce A Colaux of equal
__ depth as Ina Stendlord Compare iSon_Soliitien having a
—_ | definite quantity of lead _mitrate. The Quomtfty 1s
—_| Btertecl asthe heavy metals Limit cimd 1S expressest —
as pects of lead Chy tcight) per amillion parts of the
| test Sith Stance, ae
| The _Itmit +¢5t foa heavy metals hes been based rip.
lane teaction of the metal fon_uaith hydaogen Sulphide
|aumdex the prescaibed Conditions Of the test Coursing _
| the formation of metal Sulphides“these..Yeametial 1 Rime rr elcatin dalla lands Ueeereas 2
Lbrownish Colouxatton . The test Solution Is _compexed.
with a standard prepared using cl lead Soltition ae
—____|paxts of the Substance. The aisuial. Limit cl per TP
—{ bashers 2.0 pam a
Gtandead Golubion— Test Solution — =
meee ee TESTE
Add_1 oml_of Test Solution. |
q| Add in| of Standard galt fa
+ :
“Qilute upto 25 Pins Oe: “pilus upto ae totth
distilled qscten, ___ cite Eile toactrer eee
EAN “Adjust _pit ay woith Peace By Adjust PH B= 4 cat th
| acid Ciml ox more? acetic cietd ¢4-ml ox more).
ane ee of bychroq en uu Add to mt of Hydaagen
Sulphide Solution. _____ Sulphide Solution.
7a ‘distilled coattx upto — _ 5: Add clistilled coater upto
—} | S021 } 50-0). EES
“ee 2 ee
a oe Woubid fey,
ae turbidity produced [tne “4054 Solution.
1 | ewtth that of Stcmdord Solution —.—»— DATE
Qhaewation . The turbidity Produced fn the test Solution Is
petese hom thot af Standed “Solltto
Conclusion: The given teSt Sample Comply with Standard s—
fox _\imit test of heciuy metelS cS per _Tmnditcn=—
Phoxmacopoeia- 26lu. psa ——
=| Fox Tesk-2 2 =
Obsravation:- The turbidity produced fn the test Soltttton is —
emote thon thet of Standard Soltttion — See
Concliisfon: The bgiven test Sample does nat Complies coith
| Gtamdends fox ttmit test of hecwy metals 45 Per
Tndican Pharma (opoeicr Qolk,
