0% found this document useful (0 votes)
256 views64 pages

Molecular Biology Handwrittern Notes Transcriptin

DNA Transcription post transcriptional modifications
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF or read online on Scribd
0% found this document useful (0 votes)
256 views64 pages

Molecular Biology Handwrittern Notes Transcriptin

DNA Transcription post transcriptional modifications
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF or read online on Scribd
You are on page 1/ 64
S gTrTwr Ff ® Ss MOLECULAR BIOLOGY =: “DNA seqpence exptession 3 ot wants fo express amc when Tt wants te euphss | Genome vequiation types: ATACTSCTA ue Gene X > as Cfiss aie fe Tempotal (time specigz) | SA si BP be_exprexed Sn an Lope but net dn aduth. Monomeas — Maczomeollecudas Poflysacchasidos piofeina rurebeic acide eZ : ‘i Abgusgen -TNAJOA Ongris, AOUALD Ribose 4 Deompibose ~ Post bien On ow C-A furnwienoh palypephide us “~qour protein pivolnA —> pur of man ealing “DNA tak Can inceeose OA decrgase Hae? enpacssibre $i IQBA— PUBLISHERS worgrpublsercom H—|> peal (Ce q Ph \ “ Nucleic Aids: DNA: (1864) By Fredatok o water Soluble 2 Atenhol rich ° Tne paverpetatc with DNA . ePhesphakad sich Keown as mudlein —> Nuchel Acil e Prcidic chaeacter because ofthe pruence th _plaasphoxss Chrom osormak treovy + TIOZ'=— > Suston PUBLISHERS e C « é 6 « « @ & Use 00 ohuamrosomaas pele ered ee ee —then ae € Sane a) — \ —the _inhedlenee pattern bbs —— Senos & Aceoiding, “be tha henry. Usirnt senres amnol Fémes__howre tre samo Parateel potter . Sn 1998s 25h ss Streplococens praworia x S- ype R-+ype [vous type) q (at bad cape “oP ¢ covering) DS S-+4pe (| all mice alied) => R- bye (ell mfce aliye) [¢ backeia war weak and the trost defense Ashen, way Vv Dror _ © heat -vibted s-type -s ortee Sadue Coecause Vitullenee wos mot orp rr 00) A) eat s-type+ R-type > mice 5 dead 4 ae Cee 45 trams formation ) Heat Kiel + R- type + Paotease Shape protein denaturing. , I PUBLISHERS vworeigrpublishers.com heat Kiped + Roy pe + Afbomuacleary aatype Se (ikea : « —S mice olive SS : SS REnce , prevert BNA %s the Caargin onatecol €¢ eeaiiare Z Rv Experiments 7 R-+ype + H. Stype R~fype + ‘ = protease H.g type + € J Deow rhomuckeane € head Suddve 7 6 A ote arn - DN enpers ; ag oe | Te phoge. 4 Radioackively Labelled Phasphowar amd suiphnt 7 . : : 6 ¢ 4 4 € PUBLISHERS Ser com q Se : Za lpia | anf —Nucleoside +. P04 —> Nucleotide Podpmnests Leen, aby ons cout Rom 6» 3’ Dor b ada augs o mew welecotde allachas é on thus apc fic positien (F039; => Pyrophespha us 5 aekasedl and Gt needs on oH a: ate farm plosphodierter bond . q The 4 pokprretase need OH- gees cs pirical (oe phosphor ) Ly b/w tuo Sogats rucleaticles Suger ancl Base > Bota Nn-ghycastdic bond, side prcdces pype phosphare anol water => Porton experiment ; 4 > > > A PUBLISHERS wornigepublishers.com MtWrpaa” __ A220 T=» 20 G26 C260 € = B- form of PNA is amost obumd. few € € Assignment © Why RNA 4 mot the genetic castter © vis € e e é « ( Es sas ey eo © B dom 2 fam J ue K- TT stocking. : - > RNA fonctions as on enzyme onct Jo therefore Reacug wrot vrnstable - 5 Uvod pacsert wie Rn io Less dtable os do thyenine | DNA. => ben unstable * PwA mrutakes oko amele foster “note, that in why, RNA viruses howe ter be Se fle oad ate sg_ festa, napick_chamges oss Bete « => Buesence of. QECH wa DNA. fet: 2 ee : PUBLISHERS ‘wor igrapublishes com MTWTFS S$ RNA —— aa, Se cen bg —— eee 4 cam't be — Heal fox tong “H j —— (ee meer PNA has i Groep. Ww werhar) | > ra teh of Pov aner lesan " positron Prone fer hey do-sbypin — 2 nea E behore E afert- — Eat daniel) aj. on 7 dejrodng, q 4 5 Rj vadladiow. pnabraction eB E Seong Ba 4 bord) \ a I een a loeduetn,) rustisuens publishers MTWTFSS yn ¢ DNA replicates 1 endant - . Wha 7 - Detection tf DNA, > to cheok entity jerc: D (warrolergte) —> 260 mm © apache Te FJ DNA? o Poe phe iz = eppondiorty dube athe +e Hat Loy ao water Like macdor i ae DNA. sg — } = coe. | ©. s DNA absatption’ aoe es thin heat he NR ere des will Dun trbe Acs ernas 7. a denabues 200 ng Ju Zoong)h and toes tap 37°C __femp 87°C Beis 1 as “ (corre & concobalion) ae - “oy | eee lols ott puh Taher X“ e a 7 we ae I a Cbeause 2b & 4 prerdea) : el x ork : . Mii WT F Ss adios 3g Tas 5 Dang of DNA fi) GC content Chipse bord) sion cone. (_Law ton cone reduces dyn T= 2AT+4GC Time B7-YS °C (Af tee this t +heTm , SCG 5! AesnatV20y Tre 2fi+tap+') Tm = RfPoj +44] = ofaz+4[2] 2 16°C > 4+8 - ine Renebusstitn. : [ro e! = a iS cegeat a BO Es penta a Hybvidi aationap 7 ctr Shamd4 are combined 4 P= fs ree) Ee ee ae | ee Tgnde oben.) cena « 4 shord vin Oy samt aera « amd the Af DNA malo, dn a Matas the ‘ 244 Shamol in two dliflewnk smuess ‘ « | ciging/. \ a Southeun ‘Novthan ‘ etd 27) « CER NP se Cros U oo « Humor Mitochendato, « Genome C Herne) Mo Kephicadion, ‘i 4 - —ergticalite, ) Nuckeag wbheks ¥ * Cfurctionad eit Ue conkiofting “ti oer Zep stabifrty 3) multiple copie ate pict, “motel trots 4 2 tt Canhet _ Symthesize “all Ae Copied Acme obo come \ } Fypee —mchear, ie WS PPE ae M TW ce nt e qe ty ae ee Rss Nucleos, Be Sone ar s ner /EeamertS.. Atuome a a a ae oie REP Gene * ae

- Jene —>pnh 1QBA- ( Se ruatismens pee « Sene_farni, => Po erase ——> S| 2 @ i me tet + aiaael tenth page e) he Gene tamnsciption of A_ hen tt fonschtin € amd —hen DNA poly 4 in Soymneol_ . Gene. Krom scuip tion ef B ten Ds trdnibction € gend_then DNA poly? 1s” fomedl org. compllenity Amount DNA No of genet Power l 7 vv & v oF gamism Pc at ti T qd (on. deal emeditions) C-volur: | Amount ef DNA /hapford cole Herman —> 100 Hmes TP DNA yeast Yeast > Stimea Po 7 peas: E-calf F029, — StHmes Tt * Homan Lifies — loo Himes JP” a Cvolue ef frog >_cvalue Shy burden cvale Parade —> St 91S “Jue.imnys dat je AK Wes abe to. Unolerstandl ss tebe \ 2 => Jes Se Agrectinda (during a shaking Reuclibm) LPRPRRPPRPPRPRRPFAO84 eadeenting. “the telomeres , 7 aging STRUCTURE : a + Ki SGt Leuchen onignal dou (> & PNA further oper coiling, ected) wz os OVA wound onder wound, Ly Same dtvection Ly different clivechm Cos Bing Gn fp diveahin ade and Frew the tos) ; Gg Loop uterres eth ]2 of: —Hoiwiis DNA posto VE aes : Ciheg become tren “Apa cena OA sigomt 7 (C48 sed hes. Aeduce the | Synthesized. g iO, Leads jo —s 2 _2 ad XY 3 Histone proteins: a 20n ° Basic aminoacids (ve charg. = C > nat a H2B 4 (DNA is : x ) —_ fatco. j H3 kos) PRs = 4 Jb octamer) wis sb- I oaama Tp Eran os A creche Siu \ Onesrnale~ Ls Dike beaks Or a sing - The beads axe coed nucleosome . Back near AB Corrposed SK PAA arcornk eaghk PSs cotted Dastenes_|__ ee . JanFE GWA nekaome DNA (condensed ann aa nm Linkter DA -> Sn The fon pyA is attacked with fre pis tone py stein pacing ier Length of DNA =No St Xx 000 bip purclestome v oe Cs Cm drm —» JOnm ——» Bonm open clare ak oe NS OD) reat? Unernain tH ok ob ea Deter that ane hele Dele Sper? what one tae factors > T t 7 t — wh— a BVA 4o-llObP e e ¢ a Gf ong s0960n 5 DNA 42 hata errometin e © e e ‘ r c I PUBLISHERS ipa ti TR eo I a __ Constitutive ____— Tok wilt semaine eon af Te matured on 0% SF op dn} hone — somatirnes ev cbuamakinn option fo suite bl amo _somebrees ___ on oma off drabrochtomaktys | - Stays obwvongs in the == Por of hetrachromatin Uv U wa (they ave _presert on the. cenbomenc region) - MARS Now-| Matrin associaktd regions Ristone sags. proterins L Seoffold auouobed f vee XO orp > OOO 8 2nm lonm 230m id 4 a Aepmenk & Oni bowing, the codtvg. info protein X ae Lys wre gene one pretty x ite & Wwelie« RNP s La DTG treomy faite became aa ONE GENE OWE POLIPEPHDE WIRETHESTS: plot . RNA | x aaa men) TRNA —he e exp bemevted dD tramsbede pese) aj prypeptide. am oe Tre a Cou Aon mM RNA in tranatatel furthes, rot with vRNA ond tenp e . e —Uishon: Gtwio the Ae hpanak Sy _DNA « Bosting. ‘in foxrmation of fernchionat Rv | cistron, L»> Daf e the fonctional unit of RA a Peafetrniing. Bpecific fonctions.” : _fEx|__{e3) BNA fe [hor € « See sae ae Apa WTR IO mena ertistens ‘Ne - ‘es MTWTFSS | Ne —TranscrTPTION: ~. Polymerization of RIVA by “RNA ey, \ by using. the ‘nfarmaddon x BNA." er. ot to" ~ co ve RepGicaturn Vs tranteg LU es PHom pate © ~> ack or ome str Stamd a sdvq) LY “> Strols a) an temp cake jlo stromal ~> 1) ANTPs (Au, G0) =— > 5) BNA polymerase ae “Si Srifiation facts 3 ™ — * iv) Pummeter Seqpence es 7 Steck site [stadt Cprorneter ., 0) geft Sde , sight side Poirk of bs Geopitnce Sf Ona where 2 remeaption 0 BE em Tg) «! send of montemphte Stramoh Dat The 3! end of tran sedipton ——_ template Stomnd bubkhe t fi tHbp 9-6 bp IBA so publ i) « SS TAC 7 Es see ee ee eee Polymerization 2 « oceunta y : aea9 € 3/ AT & & g € 2 nvceetides will spoke Presphediiester bonds w each other but wilt make H_yods © with nucheoti des of temp Date strand . € Transcription Initiation: i Prormol ex Ti 2m — Steam ce Gromer | FT FLAS SG en Prot Tecogni2es FAIA polyreraae £ cS’ oe ere af of men demrplatt and 3!end of “termpehle © wf 3 Test S cg! EAS gritiation— @ TRC Z£ ATtae Jeni polymerase comes 4 binds « a Gives 4 kenpehate OAS, 4, uni telly whidk wweltokde & . ee oy ¢ you favre fo. bsving\ Oe — @ e will shift — FE or g (aig og RnR OI FARA : PustisHERs owns sam ) \e : Mh WG RS s hang oo i ls Ppa clearance ater oving 7 an = Peorroler downrdial oe BCE Prone paerr6 epg a Gi Paine OB bax ) Soe ri x Letkew UC amend — aroreedd Tre Preioms ne btoute to Senter he) teomination : De wilh keop adding. Viborwcllesheles ut Ae gk a Atop rkapence . evans 4 SeSeq v Ovgernig ction & Aenes: ri SA : et foray te ie esh Le tonined Oak ex ——Aanel Alero. 2 —~@ frra Hingke purmote, pen ne Re O- Be KN wd it oes wee —— % type = THR s : eo ae ~ Ena 23. Jovrneol ent ore ee Ree fmgrn me ee Teme Ger oem owt f ove oy Oi Ps ogres aS ciara’ @ 95h hag scar Jatin 4 feandrai cod =) PUBLISHERS Ol sew igrpulishers com, “ ee MTW.T FS S _safsit fe o3 ae ohT Ae > Ewnerryetes Acne I (Cie Fiver oy them € ch Tt OSS nk es = 6 ne hE om ot « Kove BE tx — ae € D Mdividuel emprerions DD monocithowe mPa. « Acme family 6 rs > & ona ‘piedhen cal pati ae _onpityn . Gown we 4 ok Erie Ons we 7 « get, but Stee Dene gn’ re “at et msn ¢ _phenememar RNA on Eh ‘one i Posk- Fromscuptional € modificaLiOns ' Eukanpotes (RIVA vrodlfied) Puokoryotes ee (No * rnin) 2> First trom sexip How 2 Sirroailtermenaly an _ruckeu Prom ae portation Ww ae cytasak - : Trese modprcediovs are : LEA. Se eee PUBLISHERS sews igrapublisherscom My Wor i Ss si Pe eH » f nih _ Ll is I P Ts ads ay & Hi phos abe = Cakaba pis ~~ prospheoliexte, » Ante bond sary EL Gg eCTP a a eur Zr AC-TI-P Bi ° ‘ CN > Bo Hew otter this the stanctuee rr > ym: ey JM 9 > eee cp) hes ee > salen) 5/5’ coping “BS S ! 8, “et > Nockesicle 4. q CH, Chivst Ca A > fom ena 2m Fy pe onec. template 2! ate? > oe A/S RS Csecong =) ) be sepheced Con tre Dest at teh bg ee Ay drome BLP ancl Pyst ee Jeep oP —> then seemad | ther, tivd ) Onky. three Himes methylation. uh neous and caging. vith | be done 7 PUBLISHERS : — e Afi ———— eee i % Pode =o f ee [ik Sa protetteat at 5’end) Prom Monucleare activi prevention, - Beet I obiasabis 6 Aadolition of oe ln lane [px the ra types Os CNA moolificatios ore the hoo = types thet Exond] GU ——— ng Exm> OUR Abun Gu — AtA— pe Present Prom es nA a Pfrimidine % menu. Teens ia Va g : Tone . OH | the baradaaie) “Tioatuifieah 3 ft he Reaction, Ar tal oo oad t ah eortes Ex Ben 11G,u —/—AG~ Ban / “ ) ET Ca te IRB Is Known Ee— os altenntur / Te he= as Lica Gor ss J one thon an apiceat ) eS spme Hime grutatisnn codled or ' Obbertios alse StCUR Um splicing. Toe eK damages mMTWTFSS enon air 25 due ta which pUstern en in _dfshubed — > & “Tagt olteenct. i —22 Con __sKipping. in \ 3 vd : Ou anejetiue Phtnsonenca 3 “Woncated 3 dfsavenged protein (which abs ately degrades) : oH AAG bz-2 | fer oT ed SK: 4 ou] spied U ( Sener fe ( Ge Qi 5 Ee AAG, -O# £xl -0-f- OF Ey2) i “Das tak vy Stawetute ) “on + bp SnenP roeiaats : Oy SwPNP Qy. | OS SKRNP Wop Hen | “eS Ry? : = lemnol > VisnenP (Toy vbw iG 46x BNP trem the ‘ procs is sal {1 4 oot conned nyt. spline eu ‘ — Abberont Splicing. _& Exon eipping- : rd T-2 . uf a 3 " SAS UISHRP ge U2ARR c ‘ e q e « € « I PUBLISHERS MUW TE s Ss SAS ea I - a _ la SoH (see wep re) Y aa i A. Pat onl Te U aegis é ea es SDS is caype Shi a expec’ on En op He seacer 6 Atteuratione,, ihre: Sryodolast wNIeL ds bord yt aoe ar feces, Oe ot PSE © encreles wm botjand” Fey my t THB a es PNA er: rt ae GB ape eae é dna fotms 4 QZ AL Bums of prods translated! fon, : proteins. tmne RNA T Php. pe. ( pes PUBLISHERS ‘worscigrapublishers. com, Biya |] | |° ee MTW.T FES S Eh, Li __ fi =H 4 A type Se PAST, enneteol y f pins ‘ — fo _—bcack_d] Re t eo Significance af abter natin Splicing, = Vas a Aff Vennironment Aide cplia —— alife scoJoune

SKD Ex) Ex2 v3 + gayet chet | f Ex SS (Beso In on Hin tam be enon im on8 fey) Sewornk atqublodion tvamseai ption factor. ‘ ut iso. type 9 pin whidh 4a Feiny_ to: 3 aaa { f ee N { - ackvabok ROPROREL 2 adh tex the binding Ey a eater Tp ke SRA polpmarese AEpreror 5A onwbate,| | with the prornote OA DVes enpAbyyi Sw ‘ seqpence . ee tre Stark ‘i 2 \avinimal sation Ly rckvoter ' vrontected) ; PUBLISHERS a> tb ewhonce the wauttatar wae toe ° TRANSLAT 9 2 wwwsim F mRNA Into thy polyp ® ARNA ond sdlaP omnes. axe difher sa ape eT @_ARNA fey thts > Rao os dreeerrbes) @ =m RNA acts 0% 9 tosis ee vwooniqrapsblishers. com § VAUSCUG wea 2. 4 las an _prortrrd on the NEN aed 4pem of Senthic_codle - - a 4omotit code. 2 i a PUBLISHERS MTW,TFS S rw lt, Geneli code AvG | 8 nuckeotider 4, 3 ——— 4 = 64 codons <= Qe = ense Non sense 64 3 Aengenurcr [ degenarate in Sewetic Code” mete Whew me cooler Ys &dhing By a o— Single arene cuciel Alo Headlens Gev aca Ace SG Bird one endow. doer} take uch’ part vn the ackaihy, the gist ho are wee anvelueck | TH —> t/mek cre coded by 4 d PPP FRRKRHRHERARR F Y_ (untronddel: aS Sr Ace gale eace Acca aa Ture HO UIR| first codon 9. tronstation anbodln, x & | Au anit. K Co s an Styrene * ak: STUTR wea ut fess... pte 1050 nck eps See fork iw 3UTR in i Pong sepuerrce om Kho & t Se < webb Ble ia 1apa-*\ por tv Dn ca ebficabionn peal renbehinnsnies ror — wethionnt tn Ransrvredh XN Meow ee Vag 5 yp AUG > met. Tromlation —S mena tRNA Arbo somes +. RNA > transfewing amino acids Ly single stramded” RNA_molecute —= 70 ~8omt Lonserreol (patio a pie oe) naa es és ankicocler $7 care ( Ys CeA & viprrent Hew Pre cvoclifrcadh ins ane ee ad ak the Pecey cannot be diene because frok wit the “ake snotty Slama = aac Es g-0H | ‘gf Aes? oH a it : I Meth PUBLISHERS ‘wae igrapublishers com CLT 7G MTW.TFS S J__l. Arnott B fpetictage = —>_it does_t.e_proe —> it ensorés that the cavreet armura od Genes to the tRwA 42. ena Neds : Sa So foe t Rol. cyt Phe. + ena] _ Armninoacyh tRNA ' Amine wetels O48 “total ww mig = 98 daminnacyk tRNA Sgrttre tare = Qa Spectfre eniy nes py SPeotfic amine acid.” 22 9f amy epurbotriny Comes Wr ont cook ) wean tte tale tegatrce that axtachecl 4a_wineng. Ribos: : . ‘omeg | rae i Namal | ; it epeptide bond © antitocloyn | todew formation catalyze = re wwe 4 OAT eS et Xx Bie BN. Xv ope bord d x kM x X ~ aBA- \ MTWTFSS ef fe —amivacia ( eles bora) tRNA cee = O- L G> bol > Ribos 4 ae es n peo korrpotes pie. og YRNA (3 4ypes) ae ~ 798 vd x S= Sed be onit 1 23S. 168 8 met Cheaviest) Ribosomes tn eukatyeses , a ; eukaryotes —> 4+type —- Yyos = SOS Zoid 4 ) 28S SS” S-9s__S y_ SIGNIFICANCE oF RiBOSomeES: ) 8 SniHaHion Sh trams hedion - po Proben. Synbp eat . q 2 Catalytic peopemty Pav _intbradfon 8} trom sfabin, B Ribosomes A-site> ennnnnoa cop AR abana pane HES Bom 5‘ to3! E- site > ent se TOL mRNA = > : p mlb Ribosowes with m RAIA —> polyromes , noone om 4 a of PUBLISHERS a wee wor igrapublishers.com 2-& 920 ara & Ape a ERNA oy 3 90 nee codons = 41 AACR 3 6 + RNA 7o-30 AAD Gost: Js! "© va 4 bs PP @vuva => onbcadons ag % eee 71 owe , 4s Srnctontee € jmapartomt) = ee Or antl hoo « 5 codon — ®veu q as nutter) oe 6 paQimination 5 Spogine has tha : yee cee °F eee ability +e bind J eepribey bal : th : possin€ Lbase) ; wi he Le. UG 5 we : « uh c+ RNA os 8 3 my ms” 4 7 tsa, Rv A» , Saud a oo: 3 4 S 4 (pergect ise 2 Gy : A porring ove one_juvst en se get it Blew He 4 tye positions ) Cee Verma maniacs Svs ERA i a : u : A ( pairing ot. ofS puck otiole The _impmper bare 5 anti wdon ard. Bia rales Ges todon” I ge a PUBLISHERS Po igraublishers £m MTWTFS S85 —— ff. —Adenanp —»_ desotegdion. > Snosé a) Savorable Se eee) — ee DNA — madtaens it gAT 4 _trwa U as Th Yclajy elu ane es: — =) mutations Sometimes hou a ven Lethal on @ —Anourtic thect . ' . = + + Ay Ce pet Nod. no erect G TRANSLATION: ao “, tena; £' mae AUG, AUG AUG a BNA SRN Bet oo a intel tRNA stabilizafion Ss difCerent, then the seat tRNAT 2 Joaded with methionine } Siw bo th p and” ew, 24 . Amine aeght PNA Synth tase wifR be come q fox. Ainkiotor +t RNA, amd simple tRNA > — Initiation — Etengatien —s Ternination 1QBA- ~ PUBLISHERS , swe grapublisers.com , TRANSLATION IN PRGKARYDTES: — { OTy i | ef WHEEL shined a ey yp | Pent stnitiation — WwW start Ave specdiR, ) a whee it & ( gat or end.) at e Shame Atgaime cin the Seaprence thok u being. vrecogriz@) boyy the maller —_ gubumit of ribosomal QnA. ) ; AUG, AVG ADORE GUA . a star} dodo $-0 xxxxxK AUG $-DAVA — Start don U4 et possible becaomst Start I : : exh with 4 rupiisiers ‘rwonigrapublishers com Hn MTWtres-» | = => When Loge Subunit comes omol atkaches — he im yy tRNA for methionine _cermes __ ‘te the Psite of the initiation corm plex > AU the othe, tRNA attach at the A_site while only the +t RNAY ottaches Ok P sive ) oLt other AP 3E tRNA, ERNA; —>P site direct iy ( cna eran ee a pire — EO, a2 b Tearaton cin, Bukontes aie ee Mh Polistranie a seeps S \ SG autkspl e. “singe pect _ mmoking 9 ee \ Promotor single funchionel Lash Kozak Seqperce > Avs, is a past _of¢ \ Kozak Seapronce ACCRUGC a Ss upst¥eam RES > Region other fran S” end where the binding. ores . Translation Stay from Kozak Seq pomee but Qe to etkorobic viteses ana mnitochandsind genes LE Staal fom yRES, elF ,» e&F , epf : elF; ( euraryotic ini Haben factor) CIF ue going to vecognize £' end prediona Sok i o2aic and J Yeo: lan ° Binding. of. Yibnsomes with mRNA oT. Be they ave pecif: with the S’ eo. The ae “the tape of Proleing which are going » te assemble at —S’ end. vat ¢ EP: ex MX we Ps They ave specifically GTP pounded y 2 When ribosome hemglocate £72 pAovi des Lneryy Gud went dowtinn add. on incosparated ce ane Sinwider une sh with Prony, ex ff PVP: wk Wf in PP han mans cid chmoefiBLisnens Miw.t * . |__ — & a °_These Hwee ave Speci fre ‘4 fn ewkaryotes. i BEI AA ; VAG UG A - 7 Swe Inihiation : Tey ] i oWhen J Mabuiut ottadwy then thel & Site wm PP and the Ackeasting, fockor € ottaeh ot A rite. only Bist tpn attqohes ot PABPr a7 pABPe RAR ot Re Paly A Bindtha Palen [Cstoplaronic poly A binding g Guu tins 2 me poly Aa tail. Tris Probl poy A toil is Avepnized by the PABPC. Then © ied _id_vecopnizes then a cluewlar Avaion & e Om Aue ty association bln the Sey (n, Pping- pyofeins amd PABPC ith hea = * - BOP me tke sede Gs tropbedion > e Due fo Th tle MOM erent The vi bosomal wbumitt wilh atorn, Anremb De and ren « they will uth Ain Linear the subunit aay. attouh of brad again & ith 5 Yee e Choness O- whlirabion ety Hbosomal unit ir: " Ud mele in tigarles Pom. e &: Dots civeuDarirzaton Ss mRNA ar cn Pate Kartoiey Iv —pskasioles PREPC Darks, po there _iy mb, ond eA op tow ons Avanclkion [trammenehion 2 a eceubivty ot he Some turk ond Wey ae pales ackivity, ‘ \e Yi Tris ot A Pep Weg eft “age ie = . Bord Oe of TWTES S —_Il__ Po amd 7 Yb Exengation: eF 4» y4p bounded . wb ie rage ¥ The first tRNA will assemble ot 5,99C—> transfes the P site white al) the other will neede pan n Agsembpe at 2 A site anee fe YN = PSE ond A site is again free eee) then Another amino acid cam Come . Ye > opie: brancfocedion A —>P and P—»é py Termmatron: ~ =? The slap cadon wt be recognized hy the weleasred factor wi, it A Y 22 Pest termination complere hos. Y Smal and feage subunit of ribosome? , mRNA and. sebeace facts TW 20° > This polyperbile is nefeased be “the release % faces - ns @ “8CEA -S ATPase ockvity. (ub hres ATP and che axes) ° GD Oe are t © | rhnabive forced Pony > —( dase te_the CS coripaste Wr Sap ph dual Pere ceftoplossk . bindingy ph Uae take He Trap Abiding » dinstead F Linens) y Due fo th Rabe & trompDaton oil be macs ; I runLIsHeRs Crene Expronion: a Sone X Guy Xxx Me ahanned ap Capea, v Zz! OND, Gree { Ne PIOC OTE C OES tum. yee Hards > fow 2 Qe aug batte oct dp Seeds L ne ‘ and at that protec + actim can be ii btted, ce r A uP pobynaesdse “Pa pibgmesane —>-S facet ——2—Plomsk A eae — ie RA wor igrapublishers.com MTWTES 5 iy Ppeateh ia the segulitory ALR Mrek arf A tects — prerek pct m_ te the gene ,__ PLomoler Seaprerce bipartile Sepena a 7 {Qsqn Sholicakes upsben) > harne wk NaS element b cere” TATA boy $5 % TTGACA TATAAT X (7a hca TARATT ) 5 Hh Wh De Requence axa &y pectic ame Hoe TOA yt DEUX but it will mot be Bnd arrtokions will pce . > Wherfucp “mutation ~ down wucdation (weak prorcler) Rncle) | Expt Evp . Ae there ib singke _poink mukobow Hen Ut won't Of thak fenel aay ee “= 7 “> \ LTGACA Kh of hed wy TATAAT - ae geri pshe tor —> S-Sectoss RIA - poly moiase n te a ; hilps at to = wr $ forte roa Came bye I aati Lg 802 Cena aaTnGERL 1QBA-*. , peot Twit & Ss 5 che goa — = % seal Offrects ny 7 oy Us Nine bite Die 4 cI ea © Ey 5 Biminek e [ptem Teemnina tor, pen Mecho] be ton AIT os [ate Teams Ny Geet pit tote 6 Rhaadeperdanck: Rhee telrednebion) Seueeragi —_iskas CONA Vesne sroui Like or Loop sta. Rn i? € ROT He gfe aden Sted a oni VHA LILA ee Cuaed Bern ct. tLA op htredn ‘ the mybsiol or DNA and oli asi relent 27 of musket ia im toe picion thom —ctnese — J e lotion op! lac -1 ac | | bcc ibe , Q th? ey ac=h et ae hac speton el Bene C_ ——_Jlncdnre ) peat Garadeplose Eaali= ag, Pe pourease Sachohe Lactose el thucore + gale.ctoge poet ’ at Glucore —» Ine operon —s oF E fockdhe—> Nac tperen —> ON 7 C AMO, v F isecenpaet) tin a Oe v ws Ja CURR Pog CLARE” Comat bind aon thap augy Dockse ——> A 7-4 ® (represen “Ls Sepatacting from sperntorr ) aren W Aepremor protec is : DNA binding dewnacn Ac Ailesteric printer. (tuo demarns) dese eatng® Va: Snducenble — Inducer —s na eo CAPA IN Proust =e Hinne emypren 2 Onrducotble —9 Inducer feesenrk ~s on SD Iyer absent => OFF (on this we Inducer is Joctose) @ — 2u A0£E pherent pp Pevre wilh not enprers > » = B-Sew will LAS Mitotion Matowk s Z \ ¥ sas Het a He a Revo yp ypoos (wild =p) a y : . | A WT a natenit (ovata ee. Snduceadle comb tukive ae

You might also like