Method

Method Quick Measurement

Originator Biochrom
Created 10/02/2012

Settings
Wavelengths 450 nm
Measurement Mode Single wavelength
Shaking Mode Off
Use Timing False

Results
Date and Time 15/12/2022 17:13:23
Result Validity Valid
Data Validity Valid
Operator LAPTOP-4H6TFKN2\ASUS
Instrument Biochrom EZ Read 2000
Instrument SN 135779
Firmware Version 1.1a

Layout
Plate Definition
Plate Layout Standard
1: Group 1 Sample wells
Well Name A450
A1 Sample 1.1 1.884
A2 Sample 1.2 0.090
A3 Sample 1.3 1.708
A4 Sample 1.4 0.116
A5 Sample 1.5 1.872
A6 Sample 1.6 0.121
A7 Sample 1.7 1.879
A8 Sample 1.8 0.084
A9 Sample 1.9 0.001
A10 Sample 1.10 0.001
A11 Sample 1.11 0.000
A12 Sample 1.12 0.000
B1 Sample 1.13 1.020
B2 Sample 1.14 0.090
B3 Sample 1.15 0.988
B4 Sample 1.16 0.128
B5 Sample 1.17 1.169
B6 Sample 1.18 0.106
B7 Sample 1.19 1.110
B8 Sample 1.20 0.124
B9 Sample 1.21 0.001
B10 Sample 1.22 0.001
B11 Sample 1.23 0.000
B12 Sample 1.24 0.000
C1 Sample 1.25 0.531
C2 Sample 1.26 0.093
C3 Sample 1.27 0.504
C4 Sample 1.28 0.155
C5 Sample 1.29 0.594
C6 Sample 1.30 0.110
C7 Sample 1.31 0.563
C8 Sample 1.32 0.105
C9 Sample 1.33 0.001
C10 Sample 1.34 0.001
C11 Sample 1.35 0.001
C12 Sample 1.36 0.000
D1 Sample 1.37 0.268
D2 Sample 1.38 0.091
D3 Sample 1.39 0.259
D4 Sample 1.40 0.084
D5 Sample 1.41 0.342
D6 Sample 1.42 0.083
D7 Sample 1.43 0.295
D8 Sample 1.44 0.099
D9 Sample 1.45 0.001
D10 Sample 1.46 0.001
D11 Sample 1.47 0.000
D12 Sample 1.48 U/R
E1 Sample 1.49 0.156
E2 Sample 1.50 0.110
E3 Sample 1.51 0.226
E4 Sample 1.52 0.036
E5 Sample 1.53 0.175
E6 Sample 1.54 0.086
E7 Sample 1.55 0.175
E8 Sample 1.56 0.029
E9 Sample 1.57 0.001
E10 Sample 1.58 0.001
E11 Sample 1.59 0.000
E12 Sample 1.60 0.000
F1 Sample 1.61 0.111
F2 Sample 1.62 0.098
F3 Sample 1.63 0.115
F4 Sample 1.64 0.040
F5 Sample 1.65 0.122
F6 Sample 1.66 0.095
F7 Sample 1.67 0.126
F8 Sample 1.68 0.031
F9 Sample 1.69 0.001
F10 Sample 1.70 0.001
F11 Sample 1.71 0.000
F12 Sample 1.72 0.000
G1 Sample 1.73 0.089
G2 Sample 1.74 0.074
G3 Sample 1.75 0.089
G4 Sample 1.76 0.036
G5 Sample 1.77 0.093
G6 Sample 1.78 0.097
G7 Sample 1.79 0.101
G8 Sample 1.80 0.032
G9 Sample 1.81 0.001
G10 Sample 1.82 0.001
G11 Sample 1.83 0.000
G12 Sample 1.84 0.000
H1 Sample 1.85 0.074
H2 Sample 1.86 0.090
H3 Sample 1.87 0.079
H4 Sample 1.88 0.039
H5 Sample 1.89 0.077
H6 Sample 1.90 0.079
H7 Sample 1.91 0.093
H8 Sample 1.92 0.034
H9 Sample 1.93 0.001
H10 Sample 1.94 0.001
H11 Sample 1.95 0.000
H12 Sample 1.96 U/R
 Transpose
Well Name A450 1 2 3
A1 Sample 1.1 1.884 A 1.884 0.090 1.708
A2 Sample 1.2 0.090 B 1.020 0.090 0.988
A3 Sample 1.3 1.708 C 0.531 0.093 0.504
A4 Sample 1.4 0.116 D 0.268 0.091 0.259
A5 Sample 1.5 1.872 E 0.156 0.110 0.226
A6 Sample 1.6 0.121 F 0.111 0.098 0.115
A7 Sample 1.7 1.879 G 0.089 0.074 0.089
A8 Sample 1.8 0.084 H 0.074 0.090 0.079
A9 Sample 1.9 0.001
A10 Sample 1.10 0.001
A11 Sample 1.11 0.000
A12 Sample 1.12 0.000
B1 Sample 1.13 1.020
B2 Sample 1.14 0.090
B3 Sample 1.15 0.988
B4 Sample 1.16 0.128
B5 Sample 1.17 1.169
B6 Sample 1.18 0.106
B7 Sample 1.19 1.110
B8 Sample 1.20 0.124
B9 Sample 1.21 0.001
B10 Sample 1.22 0.001
B11 Sample 1.23 0.000
B12 Sample 1.24 0.000
C1 Sample 1.25 0.531
C2 Sample 1.26 0.093
C3 Sample 1.27 0.504
C4 Sample 1.28 0.155
C5 Sample 1.29 0.594
C6 Sample 1.30 0.110
C7 Sample 1.31 0.563
C8 Sample 1.32 0.105
C9 Sample 1.33 0.001
C10 Sample 1.34 0.001
C11 Sample 1.35 0.001
C12 Sample 1.36 0.000
D1 Sample 1.37 0.268
D2 Sample 1.38 0.091
D3 Sample 1.39 0.259
D4 Sample 1.40 0.084
D5 Sample 1.41 0.342
D6 Sample 1.42 0.083
D7 Sample 1.43 0.295
D8 Sample 1.44 0.099
D9 Sample 1.45 0.001
D10 Sample 1.46 0.001
D11 Sample 1.47 0.000
D12 Sample 1.48 U/R
E1 Sample 1.49 0.156
E2 Sample 1.50 0.110
E3 Sample 1.51 0.226
E4 Sample 1.52 0.036
E5 Sample 1.53 0.175
E6 Sample 1.54 0.086
E7 Sample 1.55 0.175
E8 Sample 1.56 0.029
E9 Sample 1.57 0.001
E10 Sample 1.58 0.001
E11 Sample 1.59 0.000
E12 Sample 1.60 0.000
F1 Sample 1.61 0.111
F2 Sample 1.62 0.098
F3 Sample 1.63 0.115
F4 Sample 1.64 0.040
F5 Sample 1.65 0.122
F6 Sample 1.66 0.095
F7 Sample 1.67 0.126
F8 Sample 1.68 0.031
F9 Sample 1.69 0.001
F10 Sample 1.70 0.001
F11 Sample 1.71 0.000
F12 Sample 1.72 0.000
G1 Sample 1.73 0.089
G2 Sample 1.74 0.074
G3 Sample 1.75 0.089
G4 Sample 1.76 0.036
G5 Sample 1.77 0.093
G6 Sample 1.78 0.097
G7 Sample 1.79 0.101
G8 Sample 1.80 0.032
G9 Sample 1.81 0.001
G10 Sample 1.82 0.001
G11 Sample 1.83 0.000
G12 Sample 1.84 0.000
H1 Sample 1.85 0.074
H2 Sample 1.86 0.090
H3 Sample 1.87 0.079
H4 Sample 1.88 0.039
H5 Sample 1.89 0.077
H6 Sample 1.90 0.079
H7 Sample 1.91 0.093
H8 Sample 1.92 0.034
H9 Sample 1.93 0.001
H10 Sample 1.94 0.001
H11 Sample 1.95 0.000
H12 Sample 1.96 U/R
4 5 6 7 8 9 10 11
0.116 1.872 0.121 1.879 0.084
0.128 1.169 0.106 1.110 0.124
0.155 0.594 0.110 0.563 0.105
0.084 0.342 0.083 0.295 0.099
0.036 0.175 0.086 0.175 0.029
0.040 0.122 0.095 0.126 0.031
0.036 0.093 0.097 0.101 0.032
0.039 0.077 0.079 0.093 0.034
12
 1 2 3 4 5 6 7 8
A 1.884 0.090
B 1.020 0.090
C 0.531 0.093
D 0.268 0.091
E 0.156 0.110
F 0.111 0.098
G 0.089 0.074
H 0.074 0.090

Tube Con (pg/mL) Abs Std Abs Sample Con Sample

Std 1 100
Std 2 40
Std 3 16
Std 4 6.4
Std 5 2.56
Std 6 1.02
Std 7 0.48
Blank 0
 1 2 3 4 5 6 7 8
A 1.708 0.116
B 0.988 0.128
C 0.504 0.155
D 0.259 0.084
E 0.226 0.036
F 0.115 0.040
G 0.089 0.036
H 0.079 0.039

Tube Con (pg/mL) Abs Std Abs Sample Con Sample

Std 1 100
Std 2 40
Std 3 16
Std 4 6.4
Std 5 2.56
Std 6 1.02
Std 7 0.48
Blank 0
 1 2 3 4 5 6 7 8
A 1.872 0.121
B 1.169 0.106
C 0.594 0.110
D 0.342 0.083
E 0.175 0.086
F 0.122 0.095
G 0.093 0.097
H 0.077 0.079

Tube Con (pg/mL) Abs Std Abs Sample Con Sample

Std 1 100
Std 2 40
Std 3 16
Std 4 6.4
Std 5 2.56
Std 6 1.02
Std 7 0.48
Blank 0
 1 2 3 4 5 6 7 8
A 1.879 0.084
B 1.110 0.124
C 0.563 0.105
D 0.295 0.099
E 0.175 0.029
F 0.126 0.031
G 0.101 0.032
H 0.093 0.034

Tube Con (pg/mL) Abs Std Abs Sample Con Sample

Std 1 100
Std 2 40
Std 3 16
Std 4 6.4
Std 5 2.56
Std 6 1.02
Std 7 0.48
Blank 0