Automated ESR Analyzer

EA Series
User Manual

BIOBASE GROUP

Version 2020.08
 Contents
Chapter 1 Overview........................................................................................................................................... 2
1.1 Working Principle................................................................................................................................ 2
1.2 Major Parameters and Properties.........................................................................................................4
1.3 Product Structure................................................................................................................................. 5
Chapter 2 Installation of the Instrument............................................................................................................ 6
2.1 Requirements on Working Environment............................................................................................. 6
2.2 Installation Requirements.................................................................................................................... 6
2.3 Out of Box Audit..................................................................................................................................6
2.4 Installation of the Instrument...............................................................................................................7
2.5 Installation Acceptance........................................................................................................................ 7
Chapter 3 Basic Operation Process................................................................................................................... 8
3.1 Boot Screen.......................................................................................................................................... 8
3.2 Main Menu of Operation..................................................................................................................... 8
3.3 Descriptions of Submenus................................................................................................................. 10
3.4 Measurement Steps of the Instrument............................................................................................... 13
3.5 Simple Operation Steps..................................................................................................................... 15
3.6 ESR Curve..........................................................................................................................................16
3.7 Result Printing................................................................................................................................... 16
3.8 Barcode Reader.................................................................................................................................16
Chapter 4 Maintenance.................................................................................................................................... 17
Chapter 5 Package, Storage and Transportation..............................................................................................19
Chapter 6 Troubleshooting.............................................................................................................................. 20
Chapter 7 Safety Protection Device and Accident Handling.......................................................................... 21
Chapter 8 Appendix......................................................................................................................................... 22
Appendix 1 Flow Chart............................................................................................................................22

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 Chapter 1 Overview

EA series Automated ESR (Erythrocyte Sedimentation Rate) Dynamic Analyzer realizes quick
measurement of ESR by means of indirect measurement; its measurement result is well correlated with the
measured value by Westergren method; since the entire measurement process is fully controlled by
microcomputer, it is extremely easy and simple to operate this instrument. It not only can draw ESR curves of
the entire sedimentation process, but also can realize automatic temperature correction of ESR values as well
as storage, display and printing of erythrocyte sedimentation process curves and ESR values.

1.1 Working Principle

1. Measurement principle
Erythrocyte sedimentation rate (ESR) is a common indicator in clinical examination. Put the blood
sample collected in an ESR tube with anticoagulant inside; after mixing them evenly, vertically place the tube
on the sample holder of the instrument; due to gravity, erythrocytes sink gradually, leaving a section of
transparent plasma on the upper part of the ESR tube; the instrument determines the interface between
erythrocytes and transparent plasma by moving an IR TX-RX pair up and down; within a certain period of
time, the changes in dynamic sedimentation of erythrocytes can be measured.
2. Measurement method
The reading principle of EA series ESR Dynamic Analyzer is shown in Fig. 4-1. In the figure, C is the
movement range of the IR TX-RX pair, and L and H are respectively the lowest and highest reading ends of
the ESR tube.
During movement of the IR TX-RX pair from the lowest end (L) to the highest end (H), if IR light cannot
reach the TX, it indicates that IR light is blocked by high-density erythrocytes. Once IR light can reach the RX
through the ESR tube, the signal of the RX will tell the PC to compute the distance required to reach the
mobile terminal.
L1 represents the height of blood in the ESR tube when the time is zero (initial height of blood sample);
L2 represents the height of the sedimentation surface of erythrocytes in the ESR tube when the time is 30min;
L3 represents the height of the sedimentation surface of erythrocytes in the ESR tube when the time is 60min;
K represents the height of blood in the ESR tube when the IR TX-RX pair moves to the lowest end. K is
determined by the system of the ESR analyzer and is set in the PC.
If 1.6ml of anticoagulated whole blood is constantly put in the ESR tube, the height of blood in the ESR
tube will still change slightly with the difference of sample tube. To make up for such difference, the ESR
analyzer gives consideration to the initial height within a certain range of height and applies the percentage
principle of sedimentation to correct such difference.
(Fig. 4-1 Diagram of Reading Principle)

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 where, P: pore plate where the ESR tube is insert;
H: the highest position of the IR TX-RX pair;
L: the lowest position of the IR TX-RX pair;
R: erythrocytes;
S: transparent plasma;
C: test range;
K: distance between the bottom of the ESR tube and the lowest position of the IR TX-RX pair;
L1: initial height (when the time is zero);
L2: height of erythrocytes when the time is 30min;
L3: height of erythrocytes when the time is 60min
3. Measurement equation
The equation for calculation of 30min sedimentation percentage is expressed as
%S30=100(L1-L2)/(L1+K) (1)
The equation for calculation of 60min sedimentation percentage is expressed as
%S60=100(L1-L3)/(L1+K) (2)
According to the generalization and summarization of numerous experimental data of several hospitals
and laboratories, the calculation equation for converting 30min sedimentation percentage in the ESR tube to 1h
Westergren ESR value and converting 60min sedimentation percentage to 2h Westergren ESR value is
expressed as
Y=ax+b (3)
where, Y is the ESR result by Westergren method; x is the sedimentation percentage; a and b are constants.
That is to say, the 1h Westergren ESR value can be calculated by selecting (a1, b1) and value of %S30’, and
the 2h Westergren ESR value can be calculated by selecting (a2, b2) and value of %60’.
Calculated value of HCT (hematocrit) = height of centrifuged erythrocytes/(L1-11.32)×100%
where, 11.32 represents the height of 0.32ml of anticoagulant in the ESR tube.
4. Correct the ESR result to Westergren ESR value at the temperature of 18℃
Through temperature correction, the ESR values obtained from Eq. (3) at different temperatures can be
converted to standard Westergren ESR values at the temperature of 18℃; temperature correction adopts
interpolation correction which is automatically performed by the instrument; the correction method is shown in
Table 4-1 (Reference Table for Manual Correction). If temperature correction is not set during measurement,

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manual correction can also be carried out. Refer to the following table for manual correction:
Table 4-1: Reference Table for Manual Correction
18℃ Below 16℃ 17-19℃ 20-23℃ 24-26℃ 27-32℃
Corrected Measured value Measured Measured value Measured value Measured value
value value
Note 1 1-7/+20% 1-7/-5% 2-9/-16% 1-12/-37%
8-13/+12% 8-15/-5% 10-18/-16% 13-22/-37%
14-22/+11% 16-26/-5% 19-31/-20% 23-36/-35%
23-31/+11% Correction 27-36/-4% 32-43/-19% 37-50/-33%
32-40/+11% is not 37-47/-4% 44-58/-18% 51-64/-31%
41-50/+9% needed 48-57/-4% 59-64/-17% 65-74/-29%
51-59/+9% 58-67/-3.5% 65-72/-16% 75-87/-26%
60-67/+11% 68-77/-3% 73-84/-15% 88-98/-24%
68-76/+11% 78-87/-2.5% 85-98/-14% 99-109/-23%
77-85/+11% 88-98/-3% 99-108/-13% 110-119/-21%
86↑/+11% 99↑/-3% 109↑/-13% 120↑/-20%

The temperature correction range of EA series ESR Dynamic Analyzer is 15℃-32℃. Correction when the
temperature is below 15℃ is carried out according to that below 16℃; correction when the temperature is
higher than 32℃ is carried out according to that in the range of 27-32℃.
The recommended working temperature for the ESR analyzer is in the range of 15℃-30℃.
Note 1: The numbers in the table are the ranges of measured values; those following the slash “/” are the
data requiring correction which are expressed as a%; 18℃ corrected value = measured value of the instrument
at the actual temperature ＋/－ measured value ×a% (it is suggested to take round-off integral numbers).

1.2 Major Parameters and Properties

Application scope: measurement of ESR (measurement range: 0-140mm/h)

Analysis time: 30min and 60min are available for selection (sampling interval: 3min)
Analysis capacity: maximally 80 tests/hour
Analysis channels: 40 channels (maximally 40 samples can be loaded simultaneously for analysis)
Loading type: measurement at any time
Analysis result: Westergren ESR value (mm/h)
Temperature correction: automatic correction to the result at the temperature of 18℃
Measurement method: IR detection
Result resolution: 1mm/1h and 1mm/2h
Height range of anticoagulated blood: 50mm-64mm
Test accuracy: ±2mm/h (when ESR ≤30mm/h)
±3mm/h (when 30mm/h＜ESR≤80mm/h)
±5mm/h (when ESR＞80mm/h)
Precision: CV≤2%
Channel error: ≤±4mm/h
Timing accuracy: ±90s
Reading accuracy: 0.2mm

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 Display: 7" color LCD touch screen
Communication interface: RS232C serial interface
Printing: built-in thermal printer
Dimension of instrument: 300mm×425mm×185mm (W×L×H)
Weight of instrument: < 10kg
Supply voltage: AC 220V±22V, 50Hz±1Hz
Rated power: 60VA

1.3 Product Structure

Infrared Temperature
Control motor
photoelectric cassette detector

ESR tube PC CCU Alarm prompt

232 transmission Graphic LCD display Printer output

Fig. 3-1 Overall Structure of ESR Analyzer

开关电源
(1) PC CCU: it mainly controls the actions of each part of the instrument, and processes and calculates the
height of blood sedimentation collected by the cassette; it also completes the working procedures set in the
instrument.
(2) Switching power supply: it converts the input AC 220V voltage to each DC voltage and supplies it to
each part.
(3) LCD: it displays and outputs the measured results, displays operation prompts of the instrument, and
confirms user commands.
(4) 232 transmission: it transmits sample data to the PC.
(5) Printer output: it prints out the test results.
(6) ESR tube: blood sample is put in it.
(7) IR photoelectric cassette: it is installed with an IR TX-RX pair which is moved up and down to
determine the interface between erythrocytes and transparent plasma.
(8) Control motor: it precisely drives the IR photoelectric cassette to move up and down.
(9) Temperature detector: it detects the internal temperature of the instrument for temperature correction
of the measured results.
(10) Alarm prompt: it gives alarm prompts for misoperation of the instrument.

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 Chapter 2 Installation of the Instrument

2.1 Requirements on Working Environment

1. Make sure that the ambient environment is clean and dustless.

2. Make sure that the instrument is installed on a workbench which is stable and fixed without vibration;
vibration is not allowed for this instrument, so please keep away from any vibration source.
3. The place where the instrument is installed should be kept away from sunshine and dampness, so as to avoid
affecting normal working of the IR photoelectric detector inside the instrument.
4. Make sure that the instrument is used at an ambient temperature in the range of 10-30℃, and the maximum
relative humidity should not exceed 80%.
5. Make sure that the supply voltage is 220V  10% (198V—242V), 50Hz  2%; if the supply voltage is
beyond the range required by the instrument, please connect with an external regulated power supply.
6. Make sure that there are no EMI sources like centrifuge surrounding the instrument, and keep away from
strong EMI sources.
7. A good grounding environment is required.
8. Atmospheric pressure: 86 kPa~106 kPa.
9. This product is a precision electronic instrument; prior to installation, it is necessary to check if there are
special ground wires for medical instruments in the laboratory, and the ground resistance should be lower than
0.5Ω; or, a professional electrician can be employed to install the electric ground wires in the room.
10. Ventilation requirement
The instrument should be placed in a room with good ventilation condition, and the position where it is
placed should be kept away from direct-blow devices such as fan and air conditioner so as not to affect the
accuracy of temperature control.
11. Strong vibration should be avoided during operation of the instrument.
12. Avoid working environments with strong sunshine and plenty of dust.
13. A free space above 25cm should be kept around the instrument for placing various reagent bottles and
keeping the conduits unblocked and not extruded, which is conducive to heat dissipation of the instrument and
disconnection of the power switch.

2.2 Installation Requirements

EA series Automated ESR Dynamic Analyzer adopts a three-core power cord connected with a power
adapter; the socket must be well grounded; if the power socket is not reliably grounded, another special ground
wire should be connected to ensure safety. If the supply voltage is beyond the range required by the instrument,
please connect with an external regulated power supply.

2.3 Out of Box Audit

1. Check after unpacking

Unpack the EA series Automated ESR Dynamic Analyzer after reception; check the appearance of the
instrument to see if it is in good condition without damage; check the accessories of the instrument according
to the packing list to see if there is any missing or damaged one; ESR glass tubes should be handled with care.
In case of damage or absence of some parts or accessories, please contact the distributor or manufacturer.
2. Check the marks of product name and model of the instrument

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Check the marks of product name and model of the instrument to see if they are in compliance with the
purchase contract; in case of any discrepancy, please contact the distributor or manufacturer.

2.4 Installation of the Instrument

It is quite convenient to install EA series Automated ESR Dynamic Analyzer, but the following points should
be noted:
1. EA series Automated ESR Dynamic Analyzer adopts three-core power cord, so the power socket must be
well grounded to ensure safety and reliable working.
2. EA series Automated ESR Dynamic Analyzer should be placed horizontally to ensure that ESR tubes can
keep vertical after they are inserted into the test orifices of the instrument; meanwhile, the instrument should
be placed steadily and reliably to prevent vibration.
After the above preparations, the instrument can be energized for self-checking and system initialization;
instrument settings include date, time, working mode, initial number of measurement, temperature correction,
HCT measurement, etc.. We have the following suggestions:
(1) Please read the Instruction Manual carefully before setting the instrument.
(2) The working mode of the instrument is usually set to 30min.
(3) The instrument selects the measured value converted at the temperature of 18℃.
(4) The instrument usually sets the HCT measurement state to be ON.

2.5 Installation Acceptance

1. After the power switch is turned on, the instrument enters the waiting mode through initialization.
2. Enter each menu to operate according to the operation process in the Instruction Manual; if the operations
show completely normal results, it can be judged that the instrument works normally.
3. Perform actual tests on the quality control sample; if the test results comply with the requirements and can
be printed, it can be judged that this instrument is qualified in terms of performance,
4. After the above acceptance steps, the field after-sales service engineer should fill in the User Acceptance
Sheet and the Warranty Card, and offer comprehensive training to users.

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 Chapter 3 Basic Operation Process

After startup, the instrument will automatically enter self-checking. To make the instrument in the best
working condition, it is needed to preheat the instrument for 15min.
3.1 Boot Screen

3.2 Main Menu of Operation

The above figure is the main menu screen of the instrument; the working mode of 30min is displayed on
the bottom left screen; the working mode can be changed in “Instrument Setting”; the “Waiting State” below
represents that the instrument has no test samples or all samples have been tested; if “Working State” is
displayed, it indicates that the instrument is measuring samples. The square below is the status box of the test
sample in the corresponding position; date and time are displayed on the bottom right screen; that on the upper
right screen is the temperature correction indication box which indicates that the result of the test sample is
corrected to the value at a temperature in the range of 18-28℃; below are the function boxes of the main menu;
you can select functions by directly tapping on the screen; each number on the screen represents a channel; that
below each number represents the pace of each channel during the test. See the following figure:

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 Blue light column on the screen represents that an ESR tube is just inserted, and the instrument
detects the sample.
Green light column on the screen represents that the sample in this channel is being tested; do not
remove the ESR tube midway.
Red light column on the screen represents that the test in this channel shows abnormality; check
the sample height and the correction factor of this channel to see if they are normal.
Yellow light column on the screen represents that test of the sample in this channel is completed,
and the ESR tube can be removed.
Blue light column marked with HCT on the screen represents that HCT measurement is being
performed in this channel.
Remark: If “HCT Measurement” appears in the main menu, all sample positions are accompanied with the
HCT calculation function when the instrument performs routine ESR measurement; after ESR measurement,
the user should set HCT measurement for the sample at certain position; see “Instrument Setting” for setting of
HCT measurement. If “HCT Measurement” does not appear in the main menu, HCT measurement will not be
performed at all sample positions. During HCT measurement, blue light column marked with HCT will appear
on the screen, indicating that HCT measurement is being performed.
Note: (1) When EA series Automated ESR Dynamic Analyzer is in the working state, only the working mode
of a certain channel can be set.
(2) After instrument setting, please return to the main menu.
(3) During test, the ESR tube being tested in the instrument should not be removed.
In the main menu, tap the light column corresponding to the channel to enter the single-channel test display
interface. See the following figure:
In this interface, the user can check the measurement result (engineer’s password is required), test curve and
basic settings of each time. In case that the test mode selected for this channel is wrong, the single-channel
settings can be changed through “Setting” in this interface. The on/off state of temperature correction and HCT
measurement can also be changed in this interface. (The precondition is that test of the sample is not finished.)
See the following figure:

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3.3 Descriptions of Submenus

(1) Input Patient ID

When “Patient ID” in the main menu is pressed, the analyzer will enter the interface of “ID Editing” as
follows:

The upper buttons in the above figure are function buttons; those in the middle are the display fields of patient
ID.
“Next Page” and “Previous Page” in the function buttons have the function to select channel number; “Setting”
can be pressed to change the initial number of sample. After setting of patient ID, tap “OK” to save it, and then
exit and return to the main menu.
In the figure, “NO.” represents the test number, and “POS” represents the channel number which corresponds
to the number of sample inserting orifice in the upper part of the ESR analyzer. The user can input the required
patient ID in “ID#” according to the actual placing position of the test sample. “←” can be used to delete the
digit just input to change the number.
Note: After inputting the patient ID, please return the display screen of the analyzer to the main menu so as to
detect the status of sample position and print the ESR result and ESR curve of the sample after measurement.

(2) Data Query

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 On the function bar in the interface of “Data Query”, “Next Page” and “Previous Page” can be used to
query the results in time sequence; “Next Line” can be used to select the cursor to turn down; “1h” represents
the unit of measurement of ESR by Westergren method (mm/1h) corresponding to the working mode of 30min;
“2h” represents the unit of measurement of ESR by Westergren method (mm/2h) corresponding to the working
mode of 60min; the data below “1h” and “2h” are respectively the 1h Westergren ESR value and 2h
Westergren ESR value (for example, 034 represents that the Westergren ESR value is 34mm/1h).
“-----” represents that there is no measured value for the sample; “R” represents that 18℃ temperature
correction is carried out for the measured result of the sample. If “R” is not present, it indicates that 18℃
temperature correction is not carried out for the measured result of ESR of the sample. “Hct” represents the
result of HCT measurement. The data below “Hct” are the calculated values (e.g., 42%) of HCT: calculated
value of HCT = height of centrifuged erythrocytes/(initial height of anticoagulated blood -11.32)×100%.

In the interface of “Settings”, samples can be queried according to test sample ID or operation time, as
shown in the above figure:
“Select” on the function bar can be used to select the method for querying samples. For query according to
“ID”, only the sample ID needs to be input, and then tap “OK”; for query according to date, tap “Year”,
“Month” and “Day” by hand to set the query date, at which moment, the numeric keypad does not work.
“Increase＋” and “Decrease－” can be used to adjust the query date, and then tap “OK”.
After the sample result is queried, tap the sample ID once to enter the interface of “Result Review” to view
the test curve, analysis result and HCT value of the sample; also, “Print” can be selected to print values of this
sample. See the following figure:

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The test curve of the result is shown in the blank area on the left of the above figure; tap “Exit” to go back to
“Data Query”.
(3) Data Editing
Select “Data Editing” in the main menu to enter this interface, as shown in Fig. 6-5:

Clear All: clear all data results together. Note: Cleared results are irretrievable.
Clear History: clear the selected sample result.
Exit: go back to the main menu.
Tap the sample ID# in the interface once to enter the interface of “Modify Patient ID”, where the user can
independently changes the sample ID stored in the instrument after sample test, as shown in the following
figure:

(4) Data Communication

Select “Data Communication” in the main menu to transmit sample data to the PC or printer through 232
serial interface, as shown in the following figure:

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Currently, “Query” and “Test Data” are still under development, and their functions will be upgraded
subsequently. When “Start Transmission” is selected, the transmission progress bar will be filled gradually;
after it is fully filled, transmission of the sample data is completed.
(5) Instrument Setting
Select “Instrument Setting” in the main menu to enter the interface shown below.

The instrument provides two options of working mode: 30min (corresponding to 1h Westergren result), and
60min (corresponding to 2h Westergren result). “18℃ Temperature Correction” can be selected to turn on/off
temperature correction. “Date/time Setting” allows you to enter the interface of “Time/Date Setting”, as shown
in the following figure:

Touch the box that needs change with a finger; adjust digits by touching “﹢” and “﹣” below; tap “Save”
after inputting the digits, and then exit.
“Factory Setting” is mainly for after-sales repair and maintenance by engineers from the factory, which is
set with a password by the factory. To use this function, customers may contact engineers of the factory.
“HCT Measurement” can be selected to turn on/off HCT measurement; customers can turn it off when
HCT measurement is not needed, thus to save test time.

3.4 Measurement Steps of the Instrument

(1) Reading principle

We use a cassette composed of infrared photocells to vertically test 40 ESR tubes in a simultaneous way; a

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datum (i.e., instrument resolution) is read at a spacing distance of 0.16mm during vertical test; the test period
is 3min. The instrument will automatically test the ESR tubes after they are inserted in it. In the first test, the
time is set as “0”, and the height of blood in the ESR tube is judged; the acceptable range of height of blood set
in the instrument is 50mm-64mm (calculated from the bottom of the ESR tube); the suggested height is 55mm
(corresponding to the volume of 1.6ml which contains 0.32ml of anticoagulant and 1.28ml of whole blood).
When the height of blood in the ESR tube exceeds the acceptable range of the instrument, the instrument will
reject testing this ESR tube. When the height of blood in the ESR tube is in the acceptable range, the
instrument will test this ESR tube; in the whole process, 10 tests are in the working mode of 30min, and 20
tests are in the working mode of 60min. Please see Chapter 2 for the detailed principles of test and calculation.
(2) Sample preparation
EA series Automated ESR Dynamic Analyzer requires 1.28ml of whole blood in each sample. The test
sample blood can be directly injected into ESR tubes; 0.32ml of anticoagulant has been injected in each ESR
tube in advance to ensure proper volume of tested blood in the ESR tube; there are two marker lines on the
transparent label of the ESR tube. The total height of anticoagulant and blood injected into the ESR tube
should be between the two marker lines on the ESR tube. After injection, plug in the rubber cap for ESR tube,
and slowly turn the ESR tube upside down for 5-7 times to mix the anticoagulant and blood evenly; note that
there should be no air bubbles in the anticoagulated blood.

Blood level

Correct labeling
(3) Label
To identify the test sample in each ESR tube, a label should be stuck to each tube. Each label should
indicate the patient ID for differentiation of different test samples, and should be stuck smoothly to avoid
errors of test result due to the fact that the ESR tube cannot be inserted in the test orifice of the instrument or
fails to reach the bottom specified by the instrument. The label should be stuck to the ESR tube in the position
shown in Fig. 7-1; there are two marker lines on the label of ESR tube F, and the distance between these two
lines is 6mm; this is the position of effective height of blood when the instrument suggested height M is 55mm
(corresponding to the volume of 1.6ml which contains 0.32ml of anticoagulant and 1.28ml of whole blood, i.e.,
the position of H shown in Fig. 7-1); at this point, blood should be injected between the two marker lines to
allow the instrument to detect the height of blood in the ESR tube.
Note: If the instrument suggested height is not adopted, the total height of blood and anticoagulant should
not be beyond the range of M; in Fig. 7-1, the highest position of M is 64mm high and the lowest position is
50mm high.
(4) Sample mixing
Prior to test, the sample must be fully mixed. Mixing method: turn the ESR tube upside down for at least 5
times, or use a rotary lab mixer or special mixer.

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 (5) Sample inserting
After mixing, the sample should be immediately inserted in EA series Automated ESR Dynamic Analyzer.
Therefore, we suggest that sample mixing be carried out near the instrument. The instrument has no restriction
on when the sample can be inserted, so you may insert the sample randomly when the instrument is in the
working state or waiting state. After the sample is inserted, please pay attention to the display content in the
watch window of sample inserting positions in the main menu of the instrument; a blue light column should be
displayed in the position where the sample is inserted, indicating a new sample is inserted in this position (note
that the longest time delay after sample inserting is 2s). Otherwise, the sample may be improperly inserted, and
reinserting should be carried out.
Positions in the watch window of the main menu: 1-10 positions in four rows from left to right correspond
to 1-10 inserting orifices of the instrument from left to right; 0-4 rows from top to bottom correspond to 1-10,
11-20, 21-30 and 31-40 positions of inserting orifices of the instrument from left to right.
(6) Sample removal
When EA series Automated ESR Dynamic Analyzer is testing the sample (i.e., a green light column is
displayed in the watch window position which corresponds to the sample position), it is not allowed to remove
the ESR tube. If the ESR tube is removed at this moment, steps starting from Step 4 should be repeated for this
sample, and the green light column displayed at the corresponding position in the watch window will
disappear.
Once sample test is completed (i.e., a yellow light column appears at the corresponding position in the
watch window), the sample can be removed; then the yellow light column appears at the corresponding
position in the watch window will disappear, indicating that this position is now free and a new sample can be
inserted for test.
If HCT measurement is needed, the user can remove the sample for centrifugation after the yellow light
column appears, and then place the centrifuged sample in the original position for HCT measurement. After
measurement, a green light column will appear at this position on the screen, indicating that HCT measurement
of this sample is completed.

3.5 Simple Operation Steps

(1) Energize EA series Automated ESR Dynamic Analyzer.

(2) Check the settings in the main menu: working mode of 30min; ESR result is corrected to 1h Westergren
ESR value at the temperature of 18℃. In case of wrong settings in the main menu, please enter “Instrument
Setting”.
Note: When the instrument is in the working state, do not change the working mode of the instrument at
random.
(3) Insert the sample in the ESR tube inserting orifice of the instrument, and pay attention to the display of
the corresponding position in the watch window of the main menu.
(4) Tap “Patient ID” to enter this interface and input the patient ID (maximally 15 digits) of the
corresponding position.
(5) Storage of sample ESR values: after 30min, sample measurement is completed; the instrument
automatically stores and prints sample ESR values (sample ESR and sample ESR curve). A yellow light
column appears at the corresponding position in the watch window of the main menu, indicating that this
sample has been tested and can be removed; after the yellow light column at this position disappears, a new
sample can be inserted for test.
Note: When HCT measurement is turned on in the instrument and a yellow light column is displayed, the

15
ESR tube can be removed for centrifugation and then placed in the original sample position for measurement.
(At this moment, a blue light column marked with HCT is displayed below the sample position.)

3.6 ESR Curve

The ESR curve of EA series Automated ESR Dynamic Analyzer is drawn directly according to the
sedimentation percentages obtained from test of ESR tube every 3 minutes; therefore, there are two types of
ESR curve, namely, ESR curve in the working mode of 30mins, and ESR curve in the working mode of
60mins. The ESR curve in the working mode of 60min is shown as follows:

Fig. 7-2

1. 30-minute mode

For this type of mode, 30-minute result which is in accordance with Westergren result(--mm/1h) will be shown
on the printing paper. Meanwhile,Westergren result（--mm/2 hours) will also be shown by the caculation on the
printing paper.

2. 60-minute mode
For this type of mode, both 30-minute and 60-minute results will be shown on the printing paper, which are in
accordance with Westergren result (--mm/1h) and Westergren result（--mm/2 hours) separately.

Attention: For 60-minute result under the 30-minute mode, it is caculated by the formula. The result is
just a reference but without any clinical meaning to the doctor.

3.7 Result Printing

After completion of sample test, the printer will automatically print the ESR value (mm/h) and ESR curve
of the test sample as well as date, time, measurement number, patient ID, etc.. The format of result printed by
the printer is shown as follows:

Print Meaning

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 Sample Report Printing Title
Date：2014-05-08 Date: May 8, 2014
Pos: 002 Measurement position of ESR tube: 002
Pat-ID:7928690303 Patient ID: 7928690303(barcode）
Serial NO.:002 Sequence NO. :002
Result: 21mm/1h ; 42mm/2h (Westergren value) sedimentation 1hour/2hours
ESR curve(same as Fig.7-2) Printing curve

3.8 Barcode reader

For EA series ESR analyzer, the external barcode is only available to the customer. One barcode reader is

required before we use it. And then we connect the RS-232-2 position with the barcode reader. At this time,

the barcode reader will be on showing the red light, which stands for starting to scan barcode.

1. Scanning one by one

When we finish scanning one barcode,the barcode reader will be off and on again, which is ready for the next

barcode. And then we should insert the ESR tube into the testing channel before we scan the next one. After

this, we can start to scan the next one and insert the next ESR tube into the testing channel. Similarly, we can

scan all the other ESR tubes.

2. Scanning by one patch

We also can scan the barcode by one patch and then we insert the ESR tubes into the analyzer sequencely. But

for one patch barcode, the maxium quantity is less than 20. Only in this condition is scanning by patch

available.

Attention: The maxiumdigit of each barcode is 16.

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 Chapter 4 Maintenance
1. EA series Automated ESR Dynamic Analyzer requires no specific care or maintenance, but the working
environment should be kept dry and clean to prevent dust from going into the ESR tube inserting orifice which
may result in unreliable operation of the IR TX-RX pair inside the instrument; the ESR glass tube should be
inserted in and pulled out with care to prevent blood from flowing into the instrument due to breakage of the
ESR tube; the instrument should be kept away from sunshine; if the instrument will be idle for some time,
disconnect from power supply and cover it with the instrument cover.

2. Do not use wet cloth or corrosive liquid to clean the instrument surface to prevent the instrument from
being damaged due to the fact that such liquid flows into the instrument through the ESR tube inserting orifice.

3. Cleaning of instrument surface: use dry cloth or medical alcohol swab to clean the instrument surface.

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 Chapter 5 Package, Storage and Transportation

1. Transportation
During transportation, the instrument should be kept away from rain, snow and mechanical collision, and
should not be mixed with or transported together with corrosive substances. Marks on the packing case of this
instrument shall comply with the requirements of GB/T191-2008 Marks for Package, Storage and
Transportation. Simple shockproof facilities should be set up in the packing case, making it suitable for air,
rail, highway and sea transports. Rain and snow splash, inversion and collision should be avoided.
2. The instrument should be stored in a place or warehouse that satisfies the following conditions: ambient
temperature: 0-40℃; relative humidity: ≤93%; clean and ventilated; sunshine, rain and hazardous gases with
chemical corrosion can be avoided.

3. Special storage conditions and methods

When the device has been stored for more than 6 months, it should be taken out from the packing case
and energized for 4h; then put the instrument in the packing case again according to the direction shown on the
case and place it in the warehouse. Do not stack up instruments, and do not place them close to the ground,
walls and roof.

4. Marks for package and transportation

This way Fragile Keep away Humidity Barometric Stacking Keep away Temperature
up material from water scope pressure layer limit from scope
scope sunlight

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 Chapter 6 Troubleshooting
1. The instrument does not print after completion of test
The indicator light of the instrument printer is in the offline state; press “SET” to make the printer in the
online state (the printer indicator light is on)
2. The instrument displays “E” when an ESR tube is inserted for test, and the result printed by the printer
is “ERR”.
The height of blood is beyond the measurement range of the instrument
Please note that there are two marker lines on the label of the ESR tube, and the distance between these two
lines is 6mm; this is the position of effective height of blood when the instrument suggested height M is 55mm
(corresponding to the volume of 1.6ml which contains 0.32ml of anticoagulant and 1.28ml of whole blood, i.e.,
the position of H shown in Fig. 7-1); it is suggested that blood should be injected between the two marker lines
to allow the instrument to detect the height of blood in the ESR tube.
3. During measurement, the motor rotates constantly and there is shaking and impact sound from the
motor.
(1) The limit detector of the cassette malfunctions; it is suggested to notify the factory for repair.
(2) The left and right limit rods of the cassette are locked; it is suggested to notify the factory for repair.
4. There is no display when the instrument is energized
(1) Disconnect from and connect to the power socket to restart.
(2) Check the user power cord to see if it is normal.
(3) It is suggested to notify the factory for repair.

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 Chapter 7 Safety Protection Device and Accident Handling
1. The instrument must be connected with a ground wire which should be reliably grounded.
2. Any conductive metal object is not allowed to contact the external plug of the instrument.
3. Only professionals are allowed to open the rear cover plate and housing of the instrument.
4. To check the fuse of the instrument, the power plug must be disconnected.
5. Pay attention to the marks on the instrument; do not artificially remove the marks. Such marks include:
A. Product name and model.
B. Manufacturer’s name and trademark.
C. Rated working voltage, rated working frequency and rated input power of the product.
6. Identification of on/off state of the power switch.
“1” on the power switch indicates it is on, and “0” indicates it is off.
7. In case that any abnormal run program or failure of the instrument is found, please immediately cut off
power supply and contact the manufacturer.
8. Before communication connection to an outer computer, please confirm that the outer computer
complies with the national requirements of GB4943 Safety of Information Technology Equipment or has
safety certification mark, and that the outer computer is reliably grounded.
9. Waste liquid in used ESR tubes may contain substances that are harmful to human health; therefore,
waste liquid in ESR tubes should be disposed according to hospitals’ requirements on disposal of hazardous
materials.
10. ESR tubes should be kept safely and properly since they are made of glass; used ESR tubes should be
disposed according to hospitals’ requirements on disposal of hazardous materials.

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 Chapter 8 Appendix

Appendix 1 Flow Chart

Main menu

Patient ID Data Query Data Instrument

Data Editing
Communication Setting

Patient ID: input sample ID.

Data Query: query results, and print curves and results.

Data Edition: modify patient ID, clear patient results, and clear all
results.

Data Communication: connect to the PC via 232 serial interface,

and transmit the results to the PC.

30min/60min Working Mode: select the working mode for test.

18℃ Temperature Correction: turn on/off temperature correction.

Date/time Setting: change date or time.

HCT Measurement: turn on/off HCT measurement.

Factory Setting: mainly used for factory channel calibration.

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BIOBASE GROUP
2# building, No.9 Gangxing Road, High-tech Zone, Jinan City, Shandong Province,
China
Tel: +86-531-81219803/01
Fax: +86-531-81219804
Inquiry: [email protected]
Complaints: [email protected]
After-sales service: [email protected]; [email protected]
Web: www.biobase.cc/www.meihuatrade.com / www.biobase.com

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