IJBPAS, June, Special Issue, 2023, 12(6): 1-12

ISSN: 2277–4998

NATURAL COMPOUNDS PROFILE OF AERIDES ODORATA USING

THE GC-MS/MS TECHNIQUE

PARASTE VK1, SARSAIYA S2*, MISHRA UC2 AND SOURABH P1*

1: Faculty of Sciences, Mansarovar Global University, Sehore, Madhya Pradesh, India
2: Laboratory, CES Analytical and Research Services India Pvt. Ltd., Bhopal, Madhya
Pradesh, India
*Joint Corresponding author: Dr. Pragya Sourabh ([email protected]) &
Dr. Surendra Sarsaiya ([email protected])
Received 25th Jan. 2023; Revised 24th Feb. 2023; Accepted 8th April 2023; Available online 15th June 2023

https://doi.org/10.31032/IJBPAS/2023/12.6.1054
ABSTRACT
Aerides odorata is epiphytic and commercially available as one of the potential orchids with
great medicinal value. The investigation that we conducted yielded alkaloids. The alkaloids found in
Aerides odorata seedlings were analysed for the very first time. The majority of alkaloids were extracted
using methanol. In addition, the levels of total alkaloids in Aerides odorata and Trichoderma
longibrachiatum VKPMGU#1 were determined. The results of the GC-MS/MS analysis of Aerides
odorata and Trichoderma longibrachiatum are presented. These tables and figures indicate the peaks,
retention time (RT), Compounds/ID, Reference number, CAS, and Quality. GC-MS/MS The
identification of alkaloids was carried out by GC-MS/MS without the need for any derivatization, as
was discovered by conducting an analysis on samples of Aerides odorata to determine the presence of
total alkaloids. The seedling of Aerides odorata had a total of 57 different alkaloid peaks. GC-MS/MS
examination of co-cultured Aerides odorata samples for the presence of total alkaloids indicated that a
total of 65 alkaloid peaks were found in the seedling of Aerides odorata that had been co-cultured with
Trichoderma longibrachiatum VKPMGU#1. In addition, we introduced the latest complete
development of the biostrategies for the cultivation of endophytes from A. odorata and the integration
of bioengineered methods by emphasizing the combined omics as a powerful way to obtain novel
metabolite novel chemicals.
Keywords: Aerides odorata; Endophytes; Natural compounds; Trichoderma longibrachiatum;
GC-MS/MS

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1. INTRODUCTION
Recent ethnobotanical research on generated is the researcher's ability to work
orchids show that they have enormous effectively with the indigenous informants.
promise for treating a variety of ailments, Aerides odorata is an epiphytic species of
and the Chinese were the first to describe orchid. Endophytes may also reduce biotic
their medicinal use. India is home to 1331 and abiotic stress in plants that contain them.
species and 186 genera of orchids [1]. There Both types of fungi's hyphae entered the
is a species of vanda known as Aerides seed's general surface and encircled it as
Odorata, which is renowned for producing they grew. The development of Orchidaceae
fragrant flowers. The flowers are similar to can be encouraged by the presence of fungal
those of the foxtail Aerides odorata, and the endophytes, which work to activate soil
shrub blooms frequently. This orchid plant nutrients in the rhizosphere. In addition to
thrives in just one habitat, a form of lowland that, the impact that alterations in the total
woods with high light intensity and low number of SMs have is discussed.
humidity, yet it is highly adaptable to Orchidaceae should be safe from soil
various settings. The plants may reach a bacteria in general if they have access to a
length of one metre, have stout, branching living chemical source. A prominent and
stems, and their natural leaves are oblong ubiquitous method of seed propagation,
and lobed. The flowers range in colour from sustainable symbiotic orchid germination
white to purple and range in length from 1.5 has proven particularly important in
to 2.5 cm. Ethnobotanically, A. odorata was situations in which particular species are
used to treat a variety of ailments, including needed for the continuation of the co-
chest pain and stomach ailments, boils in the planting system [4].
ears and nose, pneumonia, and World-wide, Traditional Medicine
inflammations [2]. Endophytes were shown (TM) has embraced Natural Products (NPs)
to be tissue selective, with just a small as a type of therapy for a number of
amount of overlap occurring between the ailments. Today's practise of Modern
leaf and root tissues at the junction. There Medicine (MM) is based on the Traditional
were several species of Xylaria, each with its Medical System (TMS). The contribution of
own unique roots and leaves, which Medicinal Plants (MPs) to the development
distinguished them from the Aerides of new medications and medical materials
odorata species [3]. was crucial. Several traditional medical
Important in influencing the quality systems, among them, make reference to
of the ethnobotanical documentation various MPs. It is common knowledge that

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plants have medicinal value, and humans remedies for a range of health issues have
have utilised plants for therapeutic purposes been chosen for phytochemical
since the time of the Vedas. Ayurveda is an investigation. If successful, this research
ancient Indian medical system that might lead to the discovery of new sources
prescribes numerous plant extracts to treat a of medications of herbal origin in the near
broad variety of illnesses. In modern future [6].
medicine, phytochemicals are utilised to When A. odorata was investigated
treat a broad spectrum of diseases. Recent with Hager's reagent (H), it was discovered
developmental study on Aerides odorata, that a greater quantity of alkaloids was
including its dynamics, new knowledge of present. The purity of the alkaloids that were
endophytes, and the synthesis of secondary obtained from A. odorata was determined
metabolites (SMs) [5]. This study is one-of- with the use of Wagner's reagent (W). An
a-kind since it focuses on the naturally examination of the tannin, terpenoids,
occurring chemicals linked with Aerides steroids, quinine, and cumarin content of A.
odorata, an endophytic fungus. Natural odorata revealed that it possessed the most
compounds originating from Aerides potent orchid medications [6].
odorata and endophytic fungus should be Phytocompounds derived from A. odorata
researched in order to meet the local and have been shown to possess anti-cancer
international demand for therapeutic activity. It inhibits the growth of cancer
substances. Investigate natural compounds cells, fights infections and inflammation,
obtained from Aerides odorata and lowers blood pressure, and acts as an
endophytic fungus with the goal of meeting antioxidant [7].
the domestic and worldwide demand for This paper aims to highlight the findings of
medical chemicals. It is widely known that recent developmental studies on Aerides
Aerides odorata present a broad variety of odorata, including its dynamics, new
floral forms and attract several species of insights on endophytes, and SM formation.
foragers via the use of specialised signals. This study is unique since it focuses on the
Despite this, not enough study has been naturally occurring chemicals linked with
conducted on Aerides odorata to understand Aerides odorata, an endophytic fungus.
how floral antagonists impact the interaction Please offer facts and an explanation in
between plants and pollinators. However support of your assertion that Aerides
phytochemical research on orchids has not odorata and endophytic fungus are
yet been undertaken, so existing orchid responsible for the production of naturally
species that are currently utilised as herbal occurring chemicals.

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2. MATERIALS AND METHODS 2.2.1 Analysis for the detection of

2.1 Coculturing endophytic fungi on natural compounds
Aerides odorata seedlings in test In order to extract the natural
bottles alkaloids, a powder made from the baked-
The detected endophytic fungal and-dried stem of Aerides odorata were
mycelium of Trichoderma longibrachiatum employed. The powder was combined with
VKPMGU#1 was employed for the purpose methanol, then subjected to a two-hour boil
of quantifying natural alkaloids. In order to at a temperature of ninety degrees Celsius.
isolate the mycelium suspension, the Upon the completion of the extraction
endophytic cultures were filtered before process, the contents were filtered using
being added to Aerides odorata seedlings. whatman filter paper#42, and the resulting
After being concentrated, the Trichoderma filtrate was placed in sample tubes prior to
longibrachiatum VKPMGU#1 mycelium being analysed by chromatography.
was injected into cut marks made on 2.2.2 Plant natural compounds
terminal leaves at the point where they extraction
connect to the stem. In the trials concerning For the purpose of extracting natural
the co-culturing of seedlings, the sterile chemicals from Aerides odorata plant
cotton plug was used as a control. The samples (Stem, acquired), 150 mg of dry
control was consisting of three independent plant parts were pulverised in a mortar and
replicates performed in the identical pestle after they have been freeze-dried, and
environment but without the addition of any then they were soaked in 50 ml of methanol
endophytic fungal cultures. Following the for 12 hours while being rotated at 180 rpm.
inoculation, the test co-culturing bottles The liquid component, measuring 40 ml,
were moved into the plant growth chamber were extracted from the mixture. After
instrument, where they were kept at a separating the aqueous phase from the
temperature of 28 degrees Celsius and methanol phase, the methanol phase was
subjected to a light/dark cycle with a evaporated at 35 degrees Celsius using a
duration of 12 hours and a light intensity of rotary evaporator. Before doing the analysis,
1500 lux. At the 15-day mark, each test vial the residual residue was re-dissolved in 5 ml
was scrutinised for results. of methanol and filtered using a filter with a
2.2 Extraction and detection of pore size of 0.45 millimetres.
natural compounds from cocultured 2.2.3 Detection of natural compounds
plant samples by GC-MS/MS

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In order to identify alkaloids using Core and, TSQ QUANTIS with the help of
the GC-MS/MS, the following conditions TRACEFINDER 5.2 EFS Quan software
were kept constant at all times. They are was used. A summary of all instrument
going to be: The multi residue pesticide conditions and gradient conditions are given
analysis performed using UHPLC Vanquish in the Table 1 and 2.

Table 1: LC-MS/MS Instrument conditions.

Liquid chromatography method
Instrumentation: Vanquish UHPLC
Column: Thermo Scientific TM Accucore TM aQ (100 mm × 2.1 mm × 2.6 μm) (P/N 17326-102130)
Sample Compartment temperature: 10° C
Column oven temperature: 25° C
Mobile phase: A: 5mM ammonium formate + 0.1% formic acid in water
B: 5mM ammonium formate + 0.1% formic acid in methanol
Total run time: 15.0min

Table 2: Gradient method for GC-MSMS.

Retention time (min) Rate (°C/min) Target value (°C) Hold time (min)
0.000 0.00 40.0 1.50
1.500 25.000 90.0 1.50
8.600 25.00 180.0 0.00
28.60 5.00 280.0 0.00
35.60 10.00 300.00 5.00
35.60 StopRun

3 RESULTS AND DISCUSSION show the peaks, retention time (RT), and
3.1 Extraction and detection of Compounds/ID, Reference number, and
natural compounds from cocultured CAS from Aerides odorata and
plant samples Trichoderma longibrachiatum, respectively.
In the course of our research, the 3.2 GC-MS/MS Analysis of Aerides
extraction of alkaloids was carried out. It is odorata samples for the presence of total
the very first time that a comprehensive alkaloids
investigation of the alkaloids that may be The identification of alkaloids was
found in the seedling of Aerides odorata has made by GC-MS/MS without any
been described. The methanol extraction derivatization. Total of 57 alkaloid peaks
procedure was used to get most of the were identified (Table 3 and Figure 1) in
alkaloids out of the plant. In a similar the seedling of Aerides odorata of which
manner, the total alkaloid presence of diverse compounds namely (1) 3-Heptene,
Aerides odorata and Trichoderma (2) Benzene, (3) Ethane, (4) Benzene, (5)
longibrachiatum VKPMGU#1 was Benzene, (6) tetramethyl, (7)
investigated. The findings of the GC- Tetramethylbenzene, (8) Dodecane, (9)
MS/MS analysis are presented in Tables 3- Benzoic acid, (10) Dodecane, (11)
4 and Figure 1-2. These tables and figures Dodecane, (12) dimethylethyl, (13)

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Benzene, 1,3-bis(1,1-dimethylethyl)-, (14) Cyclohexane, (41) Octadecane, (42)

Eicosane, (15) Decene, (16) Hexane, (17) 1- Cyclohexane, (43) Nonadecane (44)
Hexene, (18) Tetradecane (19) Ethanone, Hexadecanoic acid, (45) Ascorbic acid 2,6-
(20) Dodecane, (21) Heptacosane, (22) 4,6- dihexadecanoate, (46) Phthalic acid, (47)
dimethyl, (23) Propofol, (24) Octadecane, Dendroban, (48) 1-Docosene, (49) 3-
(25) Phenol, (26) Nonane, (27) METHYL-1-PHENYL-1H-
Cyclohexane, (28) Eicosane, (29) 3- PYRAZOLO(3,4-B)QUINOLINE (50) 9-
Heptafluorobytyryloxy-6-ethyldecane, (30) Octadecenoic acid (51) Octadecanoic acid
Pyridine-3-carboxamide, (31) Hexadecane (52) Eicosane, (53) 4-
(32) O-Acetylcitric acid triethyl ester, (33) Isothiazolecarboxamide, (54) Tetradecanoic
Heptadecane, (34) Pentacosane, (35) acid, (55) 9-Octadecenamide, (56)
Pyridine-3-carboxamide, (36) Pyridine-3- Dodecane, (57) Phenol, 2,2'-
carboxamide, (37) Heptafluorobutanoic methylenebis[6-(1,1-dimethylethyl)-4-
acid, (38) Undecene, (39) Octacosane, (40) methyl.

Table 3: Total compounds at different retention time in the seedling of Aerides odorata
Peak Retention Area Pct Compounds/ID Ref no. CAS Qual
Time
1 4.3462 0.7589 3-Heptene 6500 004485-16-9 41
2 4.3948 0.9921 Benzene 14405 001758-88-9 59
3 4.4326 0.5184 Ethane 414745 000067-72-1 91
4 4.4921 0.8277 Benzene 358315 000099-87-6 94
5 4.8648 0.5389 Benzene 358385 000488-23-3 95
6 4.9188 0.8549 Tetramethyl 358383 000488-23-3 97
7 5.3294 0.26 Tetramethylbenzene; 358396 000095-93-2 87
8 5.7723 2.1442 Dodecane 382614 000112-40-3 95
9 5.8534 0.451 Benzoic acid 369585 000119-36-8 95
10 6.2477 0.3383 Dodecane 66080 031295-56-4 72
11 6.3557 0.5974 Dodecane 398678 061141-72-8 87
12 6.4476 0.533 dimethyl- 398678 061141-72-8 92
13 6.5502 7.9078 Benzene 393902 001014-60-4 95
14 6.8095 0.3789 Eicosane 430875 000112-95-8 94
15 7.1552 0.9903 Decene 25942 074630-23-2 52
16 7.2633 1.5119 Hexane 12319 000921-47-1 46
17 7.3713 1.1166 1-Hexene 6551 003404-77-1 38
18 8.3167 2.2465 Tetradecane 398653 000629-59-4 96
19 8.8677 1.2437 Ethanone 376964 001009-61-6 93
20 9.1107 0.5369 Dodecane 55993 061141-72-8 64
21 9.2134 0.6105 Heptacosane 165301 000593-49-7 64
22 9.3376 0.3708 4,6-dimethyl- 55993 061141-72-8 74
23 9.3809 1.4903 Propofol 41614 002078-54-8 59
24 9.5321 0.8622 Octadecane 422273 000593-45-3 91
25 9.7482 7.9477 Phenol 402567 000096-76-4 95
26 9.8724 0.5404 Nonane 27253 017302-23-7 35
27 9.9859 0.5332 Cyclohexane 11235 002234-75-5 43
28 10.0615 0.6838 Eicosane 113489 000112-95-8 64
29 10.1966 0.6266 3-Heptafluorobytyryloxy-6- 165607 1000215-97-2 43
ethyldecane
30 10.3208 0.3153 Pyridine-3-carboxamide, 112295 288246-53-7 53
oxime
31 10.7314 1.4407 Hexadecane 412222 000544-76-3 98
32 11.466 0.5965 O-Acetylcitric acid triethyl 135411 000077-89-4 78
ester

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33 11.855 0.6261 Heptadecane 417585 000629-78-7 98

34 12.0062 0.4303 Pentacosane 446033 000629-99-2 90
35 12.3357 0.5886 Pyridine-3-carboxamide, 112295 288246-53-7 59
oxime
36 12.4222 0.484 Pyridine-3-carboxamide, 112295 288246-53-7 90
oxime
37 12.5194 0.6744 Heptafluorobutanoic acid, 180157 1000282-97-3 78
heptadecyl ester
38 12.6274 0.6475 Undecene 44652 055170-93-9 46
39 12.7247 0.4595 Octacosane 169721 000630-02-4 38
40 12.8651 0.3067 Cyclohexane 183899 055521-27-2 10
41 12.9354 0.8049 Octadecane 422272 000593-45-3 96
42 12.984 0.3528 Cyclohexane 25967 062238-33-9 43
43 14.2426 0.3003 Nonadecane 426701 000629-92-5 86
44 14.5668 2.3781 Hexadecanoic acid 422834 000057-10-3 98
45 14.6316 0.4666 Ascorbic acid 189413 028474-90-0 46
46 14.6694 1.2915 Phthalic acid 175178 1000308-91-3 72
47 14.9881 38.216 Dendroban 424944 002115-91-5 94
48 15.1069 0.3585 Docosene 129888 001599-67-3 83
49 15.2312 0.2684 3-METHYL-1-PHENYL-1H- 138667 000894-88-2 86
PYRAZOLO(3,4-
B)QUINOLINE
50 16.2522 4.2712 9-Octadecenoic acid 430800 000112-80-1 99
51 16.4466 1.945 Octadecanoic acid 431420 000057-11-4 97
52 16.7708 0.5284 Eicosane 430880 000112-95-8 91
53 17.3164 0.3938 4-Isothiazolecarboxamide 123345 037572-35-3 90
54 17.6999 1.33 Tetradecanoic acid 277643 002040-64-4 89
55 18.3967 0.7836 9-Octadecenamide 112657 000301-02-0 70
56 18.7317 0.6452 Dodecane 382625 000112-40-3 92
57 19.153 0.6831 Phenol, 2,2'-methylenebis [6- 444204 000119-47-1 98
(1,1-dimethylethyl)-4-methyl

Figure 1: GC-MS chromatogram of Aerides odorata samples for the presence of total alkaloids

3.3 GC-MS/MS Analysis of co- cocultured seedling of Aerides odorata with

cultured Aerides odorata samples for the Trichoderma longibrachiatum VKPMGU#1
presence of total alkaloids of which diverse compounds namely (1)
Total of 65 alkaloid peaks were Ergosterol, (2) Acetic acid, (3) Ethane, (4)
identified (Table 4 and Figure 2) in the Benzene, (5) Benzene, (6) Dodecane (7)

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Undecane, (8) Nonane, (9) Benzene, (10) (39) Benzyl (dideuterated) methyl ether,
Nonane, (11) 2-Decene, (12) Hexane, (13) (40) Hexadecanoic acid (41) Palmitic acid;
Cyclohexane, (14) Tetradecane, (15) (42) n-Hexadecoic acid, (43) n-
Ethanone, (16) Eicosane, (17) 3-methyl-5- Hexadecanoic acid, (44) Dendroban-12-one,
propyl, (18) Hexadecane, (19) Phenol, (20) (45) Heptadecane, (46) Heptadecane, (47) 9-
Pentadecane, (21) Phenol, (22) 4,5- Hexacosene, (48) Oleic Acid, (49)
dimethyl-, (23) Cyclohexane, (24) 10- Octadecanoic acid, (50) Stearic acid, (51) 9-
Methylnonadecane, (25) 1-Hexene, (26) Octadecenoic acid, (52) 9-Octadecenoic
Pyridine-3-carboxamide, (27) Hexadecane, acid, (53) Cyclopropaneoctanal, (54)
(28) Propanetricarboxylic acid, (29) Carbonic acid, (55) Heptadecene, (56)
dihydro-2'-(morpholin- 4-yl)-5',7'- Hexadecane, (57) Octadec-9-enoic acid,
dinitrospiro, (30) Nonacosane, (31) (58) Pentatriacontene, (59)
Tetradecene, (32) 5-Undecene, (33) Octadecenamide, (60) Nonadecatriene, (61)
Muscimol, (34) Oxalic acid, (35) Octadecenal, (62) Dodecanedioic acid, (63)
Octadecane, (36) NEOPHYTADIENE; (37) Octadecenal, (64) Trimethyltritriacontane,
Benzenedicarboxylic acid, (38) Tricosane (65) Cyclohexanecarboxylic acid.

Table 4: Total compounds at different retention time.

PK RT Area Pct Library/ID Ref CAS Qual
1 4.3461 0.1199 Ergosterol 170280 000057-87-4 53
2 4.3894 0.1547 Acetic acid 116808 065611-32-7 47
3 4.4326 0.2 Ethane 81288 000067-72-1 91
4 4.8701 0.1159 Benzene 358395 000095-93-2 90
5 4.9188 0.2018 Benzene 358385 000488-23-3 90
6 5.7722 0.429 Dodecane 382630 000112-40-3 87
7 6.3557 0.1345 Undecane 47346 017312-82-2 90
8 6.4475 0.1189 Nonane 27252 017302-28-2 64
9 6.5555 2.6814 Benzene 393902 001014-60-4 95
10 6.8148 0.134 Nonane 46170 062185-53-9 80
11 7.1551 0.2672 2-Decene 25942 074630-23-2 49
12 7.2686 0.3341 Hexane 12319 000921-47-1 50
13 7.3766 0.3154 Cyclohexane 25982 081983-71-3 49
14 8.3166 0.8004 Tetradecane 398647 000629-59-4 98
15 8.8676 0.2954 Ethanone 376962 001009-61-6 94
16 9.1107 0.1835 Eicosane 430875 000112-95-8 87
17 9.2133 0.1914 3-methyl-5-propyl 46168 031081-18-2 53
18 9.343 0.1118 Hexadecane 113507 000638-36-8 72
19 9.3808 0.3857 Phenol 41646 002934-05-6 53
20 9.5482 0.4321 Pentadecane 405601 000629-62-9 97
21 9.7535 2.6756 Phenol 402563 000096-76-4 96
22 9.8724 0.137 4,5-dimethyl 27253 017302-23-7 46
23 9.9858 0.1615 Cyclohexane 25967 062238-33-9 43
24 10.0668 0.1491 10-Methylnonadecane 113493 056862-62-5 72
25 10.2019 0.1588 1-Hexene 6551 003404-77-1 45
26 10.3261 0.1369 Pyridine-3-carboxamide 112295 288246-53-7 59
27 10.7313 0.6155 Hexadecane 412222 000544-76-3 98
28 11.4713 0.2008 Propanetricarboxylic acid 428966 000077-93-0 86
29 11.8603 0.192 dihydro-2'-(morpholin-4- 252160 130138-26-0 87
yl)-5',7'-dinitrospiro
30 12.0061 0.1233 Nonacosane 452986 000630-03-5 87

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31 12.4275 0.123 Tetradecene 54533 041446-60-0 58

32 12.6382 0.1721 5-Undecene 34990 143185-91-5 35
33 12.73 0.1325 Muscimol 6883 002763-96-4 14
34 12.865 0.1169 Oxalic acid 154534 1000309-24-4 45
35 12.9407 0.3119 Octadecane 422268 000593-45-3 98
36 13.3728 0.3483 NEOPHYTADIENE 429805 000000-00-0 98
37 13.7348 0.212 Benzenedicarboxylic acid 429636 000084-69-5 86
38 14.2479 0.2603 Tricosane (CAS); n- 441203 000638-67-5 95
Tricosane
39 14.2966 0.1124 Benzyl (dideuterated) 5235 000000-00-0 89
methyl ether
40 14.3884 3.7901 Hexadecanoic acid 422834 000057-10-3 99
41 14.4208 4.037 Palmitic acid 422835 000057-10-3 99
42 14.5127 10.4515 n-Hexadecoic acid 422834 000057-10-3 99
43 14.5667 7.8091 n-Hexadecanoic acid 422834 000057-10-3 99
44 14.9934 10.9861 Dendroban-12-one 424944 002115-91-5 93
45 15.6201 0.8128 Heptadecane 85525 000629-78-7 64
46 15.6633 0.2679 Heptadecane 85525 000629-78-7 60
47 15.8848 0.2586 9-Hexacosene 255379 071502-22-2 90
48 16.2629 22.4998 Oleic Acid 430796 000112-80-1 99
49 16.4412 8.3246 Octadecanoic acid 431424 000057-11-4 98
50 16.5438 0.8649 Stearic acid 431420 000057-11-4 90
51 16.5816 0.9036 9-Octadecenoic acid 430795 000112-80-1 93
52 16.6248 1.4121 9-Octadecenoic acid 430800 000112-80-1 95
53 16.9868 1.5059 Cyclopropaneoctanal 165614 056196-06-6 98
54 17.2136 1.5599 Carbonic acid 156874 1000314-55-8 52
55 17.2406 1.4993 Heptadecene 84041 006765-39-5 83
56 17.4567 0.8052 Hexadecane 181759 004113-12-6 49
57 17.6728 2.1705 Octadec-9-enoic acid 113356 1000190-13-7 15
58 17.8133 0.2582 Pentatriacontene 183897 006971-40-0 44
59 18.3912 0.4156 Octadecenamide, (Z)- 112657 000301-02-0 78
60 19.0665 0.2456 Nonadecatriene 100228 1000131-11-3 38
61 19.1151 1.645 Octadecenal 148195 002423-10-1 92
62 19.4176 0.3131 Dodecanedioic acid 78353 000693-23-2 27
63 19.7525 2.4053 Octadecenal 426018 058594-45-9 90
64 20.0065 0.4386 Trimethyltritriacontane 309322 081469-02-5 89
65 20.0767 0.3728 Cyclohexanecarboxylic acid 65800 027948-10-3 38

Figure 2: GC-MS chromatogram of co-cultured Aerides odorata samples for the presence of total alkaloids

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 IJBPAS, June, Special Issue, 2023, 12(6): X-X
ISSN: 2277–4998

The current experiment was carried out antioxidant. There are a number of other
by Paul et al. [8], and their focus was on the chemicals in addition to those that have
presence of a variety of alkaloids in orchid already been mentioned. In addition to
tissue. Some of them ought to have having anticancer capabilities. Squalene has
applications that are useful. On the other been shown to inhibit the growth of cancer
hand, the effectiveness of creating cells and is effective in warding off a variety
antibacterial activity may differ from of diseases that can affect both humans and
species to species. In a similar manner, the animals. For example, 1,3 propanediol may
active principle may likewise be different be utilised in a variety of settings. In
from one species to another. Phytochemical addition to its many other applications, it is
studies and bioactivity testing for anti- utilised in the manufacture of adhesives,
inflammatory and anticancer activity grease, antifreeze, and medicines. Because
indicated the intriguing compounds' of its oily nature, hexadecan-1-ol is
potential usefulness in these areas [9]. frequently utilised in the cosmetic industry
Aerides odorata Lour. This was determined as an emulsifier in skin creams and lotions
by comparing the amount of total phenolic [1]. It has been discovered that A. odorata
compounds found in each of the two includes naturally occurring alkaloids and
extracts. A total of 52 different endophytes compounds that have antibacterial,
were identified in the leaves, stems, and antioxidant, and anticancer properties.
roots of the Aerides plant. It was discovered 4 CONCLUSIONS
that the plant's leaves contained 27 times GC-MS/MS Analysis of Aerides
more fungal endophytes than either the roots odorata samples for the presence of total
(17) or the stem tissue (8) [10]. alkaloids revealed that the identification of
Phytocompounds derived from A. odorata alkaloids was made by GC-MS/MS without
have been shown to possess anti-cancer any derivatization. Total of 57 alkaloid
activity. It inhibits the growth of cancer peaks were identified (Table 3 and Figure
cells, fights infections and inflammation, 1) in the seedling of Aerides odorata. GC-
lowers blood pressure, and acts as an MS/MS Analysis of co-cultured Aerides

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Paraste VK et al Research Article

odorata samples for the presence of total [4] Hasnu S, Deka K, Saikia D, Lahkar
alkaloids revealed that total of 65 alkaloid L, Tanti B (2022). Morpho-
peaks were identified (Table 4 and Figure taxonomical and phytochemical
2) in the cocultured seedling of Aerides analysis of Vanilla borneensis Rolfe
odorata with Trichoderma longibrachiatum a rare, endemic and threatened
VKPMGU#1. orchid of Assam, India. Vegetos
5 ACKNOWLEDGMENTS 2022; 35: 381-91.
The authors are grateful to the [5] Uppala PK, Gudhanti SNKR,
Mansarovar Global University, Sehore, Alavala RR, Prasanth D, Vangoori1
Madhya Pradesh, India and CES Analytical Y (2022). Antioxidant and
and Research Services India Pvt. Ltd., Hepatoprotective Activity of
Bhopal, Madhya Pradesh, India for their Aerides odorata Lour. on Alcohol
support and cooperation. We are also Induced Liver Damage in Rats.
thankful to our laboratory colleagues and Indian J Pharm Sci
research staff members for their constructive 2022;84(5):1257-1268.
advice and help. [6] Akter M, Huda MK and Hoque MM
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