T.A.
MARRYSHOW COMMUNITY COLLEGE
SCHOOL OF ARTS, SCIENCE AND PROFESSIONAL STUDIES
DEPARTMENT OF NATURAL SCIENCE
YEAR 2 BIOLOGY
LAB # 2
SKILL: Drawing
TITLE: Dicotyledonous leaves
AIM: To study the internal structures of dicot leaves using the compound microscope.
THEORY:
The leaves are often structurally different from other plant organs. The dicot leaf consists of one
or more layers of densely packed parenchyma cells rich in chloroplasts, and a variety of
supporting tissues that allow this thin layer of cells to perform photosynthesis efficiently. The
dense layer of parenchyma is called palisade mesophyll. Its cells are stacked tightly together,
with their long axes perpendicular to the top surface of the leaf. Each cell is directly exposed to
light at its top end. The bottom end is in contact with a loosely packed layer of spongy
mesophyll, with numerous air spaces between the cells. This airy tissue allows rapid diffusion of
atmospheric gases. The palisade and spongy mesophyll layers are sandwiched between two
transparent layers of epidermis. Veins rise from the vascular bundles of the stem and enter the
leaf through the leaf stalk or petiole. In dicots the veins then branch out in a net-like pattern to
reach all parts of the leaf blade. Each vein contains xylem, which brings in water and simple
minerals, phloem, which carries sugars from the photosynthetic cells to all other live cells in the
plant, and fibres which help to support the otherwise flimsy leaf blade.
MATERIALS:
Compound microscope
Slide of typical dicot leaf
Drawing materials
PROCEDURE:
1. Examine the slide using a microscope.
2. Make a plan drawing of the leaf.
3. Make a detailed annotated drawing of each of the following:
a. An epidermal cell
b. A palisade mesophyll cell
c. A spongy mesophyll cell