Healthy Whole-Wheat Muffins with Grape Pomace
Healthy Whole-Wheat Muffins with Grape Pomace
Article
Application of the Just-About-Right Scales in the
Development of New Healthy Whole-Wheat Muffins
by the Addition of a Product Obtained from White
and Red Grape Pomace
Miriam Ortega-Heras , Inmaculada Gómez * , Sara de Pablos-Alcalde and
María Luisa González-Sanjosé
Facultad de Ciencias, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain; [email protected] (M.O.-H.);
[email protected] (S.d.P.-A.); [email protected] (M.L.G.-S.)
* Correspondence: [email protected]; Tel.: +34-947259074
Received: 1 August 2019; Accepted: 12 September 2019; Published: 17 September 2019
Abstract: The aim of this study was to evaluate the adequacy of sensory attributes, by the use
of just-about-right (JAR) scales and penalty analysis, of new healthy whole-wheat muffins with
high fibre content due to the addition of two products obtained from white and red grape pomace.
Furthermore, the nutritional, colour and texture properties of the muffins were evaluated. For that, five
formulations of whole-wheat muffins were studied: a control muffin made with 100% whole-wheat
flour and muffins made with 10 and 20% of white and red grape pomace product, respectively.
The incorporation of grape pomace products in the recipe led to muffins with "high-fibre content",
which would imply healthy properties in these muffins. The hardness and chewiness increased
whereas the springiness, cohesiveness, resilience and colour parameters decreased when the grape
pomace products were incorporated. The sensory analysis indicated high levels of acceptability
of the muffins that incorporated white and red grape pomace products at concentrations of 10%.
The penalty analysis showed that none of the attributes evaluated affected the acceptability of the
new muffins significantly; only the darker colour of the muffins caused by the red grape pomace
product could affect the acceptability, although this fact was not clearly demonstrated.
1. Introduction
Nowadays, there is a high demand for functional foods and the supply of healthier dietary
options on the market is increasing. The reformulation of existing products is a viable alternative to
develop new products. In fact, many healthier foods have been developed by the addition of functional
ingredients, improving original recipes. However, the modifications induced by the reformulation
do necessitate the evaluation of different aspects to assert the new product’s success. Firstly, the cost
of production should be reasonable and have low repercussion on the final price of the new product.
Furthermore, safety should not be altered, shelf life should not be reduced and sensory properties
should be satisfactory to consumers. In summary, the new product has to meet consumers´ needs and
expectations. For that reason, its acceptability has to be evaluated [1]. In addition, the knowledge
about the consumers’ perception of the different sensory attributes and of the contribution of each
attribute to the acceptability is a very useful information to identify potential improvements of the
recipe to be moved closer to the “ideal” product [2].
In classic sensorial science, sensory characterization has been traditionally carried out by trained
judges. However, the training and maintaining a sensory panel can be quite expensive and time
consuming. For that reason, sensory sciences have developed more flexible and rapid sensory tools that
give extra agility to sensory characterization, both in terms of timing and training requirements [3]. Some
of these new techniques are based on the evaluation of individual attributes such as Check-All-That
Apply (CATA) and flash profiling. Other methods are based on the evaluation of global differences,
as with sorting and mapping; others still, such as just-about-right scales (JAR), not only describe the
product but also allow to determine the ideal level of the product attributes [4]. When the JAR scales
are used, consumers are asked to rate the intensity of an attribute, indicating whether the intensity of it
is just about right, too strong or too weak compared to their internal ideal [5].
Within the most-used techniques to get information about consumers’ perception of the sensory
attributes of a product are the JAR scales and attribute liking questions [2]. The JAR scales usually
have five points to assess whether there is much less, much more or a “just-about-right” level of an
attribute [6] and are a reliable tool to study the adequacy of sensory attributes [7]. Furthermore, it
should be noted that a penalty analysis is used in order to gain an understanding of the attributes that
most affected liking ratings [8]. This method provides guidance for product reformulation or a better
understanding of attribute adequacy in relation to liking in terms of direction, with the assumption
that the maximum hedonic score will occur at the “just-about-right” point [9,10].
Muffins are one of the most frequently consumed bakery products. Moreover, fibre is, among
others, a healthy functional ingredient that has had a high impact over recent decades [11]; therefore,
adding fibre to muffins without affecting the sensory characteristics is a significant challenge. Different
studies have determined that grape pomace, a winemaking by-product, is an excellent source of
dietary fibre to use in nutraceutical, medical and alimentary applications [12,13]. There are numerous
food matrices that incorporate different additives obtained from white and red grape pomace (bread,
biscuits, muffins, yoghurt, cheese, sausages, seafood, purée, etc.) at levels that range between 0.1% in
cheese to 100% in infusions [14]. However, one of the problems associated with the incorporation of
these products in food matrices is that they can modify sensorial properties [14]. In this sense, Acun
and Gül [15] demonstrated that the use of flours prepared with grape-skin extract at concentrations of
5% in biscuits improved their acceptability, but high levels of this extract led to consumer rejection, due
to a bitter taste and a darkening of the product. Therefore, when products obtained from winemaking
by-products are used in the reformulation of new products, it is necessary to determine the maximum
acceptable dose and to evaluate all those organoleptic properties that can affect the global acceptability
of the product by the consumer.
It should be highlighted that, although recent consumer trends have embraced the concepts of
sustainability and health, the sensory properties of foods are the most important reason people eat
the foods they eat [16]. Therefore, the main aim of this work was to evaluate the sensory properties
and adequacy of sensory attributes, by the use of JAR scales and penalty analysis, of new healthy
whole-wheat muffins. Furthermore, their nutritional, colour and texture properties were evaluated.
2.1. Ingredients
The ingredients used in the preparation were whole-wheat flour, sunflower oil, brown sugar,
semi-skimmed milk, baking powder and salt, which were obtained from a local supermarket.
The products used in this study were obtained from white and red grape pomace and were
named WP (white product) and RP (red product), respectively. They were obtained according to the
patented method CCP:ES2524870 [17]. Briefly, dried grape pomace free of seed was milled, sieved
(particle size <250 µm mesh) and microbiologically stabilized, obtaining powder products useful to be
used as food ingredients. Obtained products were kept under vacuum and darkness until their use.
The composition of the RP was: total dietary fibre (48.6%), total protein (14.4%), total lipid (3.7%) and
ash (14.4% of dry matter; potassium: 43.3 mg/g of dry matter; total phenolic content: 25.9 mg gallic
acid/g) [9]. Moreover, the composition of the WP was: total dietary fibre (53.3%), total protein (14.4%),
Foods 2019, 8, 419 3 of 15
total lipid (3.7%) and ash (9.8% of dry matter; potassium: 36.5 mg/g of dry matter; total phenolic
content: 13.8.9 mg gallic acid/g) [18].
Table 1. List of the ingredients of the control (C) and the reformulated muffins.
The different formulations of muffins were prepared with a mixer (KitchenAid KSM90, Benton
Harbor, MI, United States). Firstly, the eggs were beaten until they peaked. Then, the milk and the
sunflower oil were quickly added, ensuring that all the ingredients were well mixed. Subsequently, the
mixture of whole-wheat flour, yeast, and the seasoning, or the salt in the case of the control muffins,
were added and beaten at high speed for 5 min. Finally, the mixture was beaten at high speed for 3 min
and spooned into paper baking cups. The selected baking temperature was 180 ◦ C for 17 min. After
baking, the samples underwent packaging with a partial vacuum of 30% in polypropylene bags and
they were stored at room temperature for 24 h until the corresponding analysis.
Three batches, each one with 40 muffins, of each of the different formulations of the study were
prepared on three different days.
2.5. Colour
Instrumental measurement of the colour of the muffins was performed at 24 h after baking,
employing a Konica Minolta CM-2600d spectrophotometer (Konica Minolta Business Technologies Inc.,
Tokio, Japan). The D65 illuminance and 10º standard observe were selected. The colour coordinates
were determined in the CIELAB colour space, expressing the results in terms of L* (lightness), a*
(redness) and b* (yellowness).
Crust and crumb colour were measured by duplicate on five different muffins by batch and
formulation. For the measure of the crumb colour, each muffin was cut in half parallel to the base.
the objective of establishing between which samples there were statistically significant differences.
The Statgraphics Centurion XVII.I software (IBM Corp., Armonk, NY, USA) program was used.
The JAR results were analysed by PA to identify potential directions for product improvement on
the basis of consumer acceptability by highlighting the most penalizing attributes in liking terms [10].
3. Results
Table 2. Nutritional composition (mean ± standard deviation) of the control (C) and the
reformulated muffins.
Total Dietary
Sample Moisture (%) Protein (%) Fat (%)
Fibre (g/100 g)
C 14.4 ± 2.3 a 6.92 ± 1.81 a 26.7 ± 2.2 a 5.67 ± 0.44 a
WP-10 14.2 ± 0.9 a 5.77 ± 0.46 a 31.0 ± 3.9 b 9.56 ± 0.55 c
WP-20 14.1 ± 2.3 a 5.99 ± 0.96 a 32.4 ± 1.2 bc 11.9 ± 0.3 d
RP-10 14.7 ± 2.8 a 5.54 ± 0.33 a 33.3 ± 1.9 bc 8.24 ± 0.64 b
RP-20 14.7 ± 3.9 a 5.42 ± 1.19 a 33.8 ± 1.1 c 11.2 ± 0.7 d
WP-10: muffin with 10% of white product (WP); WP-20: muffin with 20% of WP; RP-10: muffin with 10% of red
product (RP); RP-20: muffin with 20% of RP. Different letters in the same column denote significant differences
between products. Least Significant Difference (LSD) test and p-value < 0.05.
Moisture was similar in the five types of muffins, which can be explained because the grape pomace
products used are not very hygroscopic. This result is contrary to those described by Bender et al. [25],
who found higher moisture values in muffins elaborated with white grape-skins. However, the
difference is easy to explain considering that they used products containing sugars, whereas the
products used in this study were free of sugars. Values of protein content were not statistically different.
However, it is possible to point out that qualitatively, muffins with red or white product showed a
lower quantity of proteins. This fact agrees with a dilution effect, mainly due to the fibre contribution
of the product (ranged from 48% to 53%, [12,18]). In fact, all the muffins elaborated with the products
presented higher concentrations of fibre than the control muffin. These results agree with others found
by others authors in different bakery products [15,25,26]. It should be highlighted that, according to
Regulation (EC) No 1924/2006 of 20 December 2006 [27], the claim “high in fibre” may only be used
if the product contains a minimum 6 g of fibre per 100 g or 3 g of fibre per 100 kcal. Therefore, the
muffins elaborated with the grape pomace products could be labelled with the claim “high in fibre”.
The product addition also modified the fat levels, being higher in muffins elaborated with red
or white pomace products. This fact is mainly due to the fat contribution of the grape product. Fat
content of the product was ranged from 3.3 to 3.8% [12,18], which is higher that the fat content of the
flour (1.8% according to brand ingredient information). Acun and Gül [15] observed similar results in
biscuits fortified with a grape-skin extract.
Table 3. Height increase (mm) and weight loss (%) (mean ± standard deviation) of the control (C) and
the reformulated muffins.
Control muffins showed the highest height increase, which decreased as the percentage of added
grape pomace product increased. This may be due to the fat and the fibre present in the seasoning,
which affected the structure of the dough, having a negative influence on CO2 retention [28]. Similar
results were obtained when date fruit were incorporated to muffins [29]. Regarding the percentage of
weight loss (Table 3), a similar effect was observed in the muffins with a 20% of the product, leading to
comparable results to those found by Bender et al. [25] when adding products obtained from red and
white grape-skins in the elaboration of muffins.
3.3. Colour
Colour parameter values were statistically different (p < 0.5) among samples (Table 4). The muffins
elaborated with the grape pomace products were darker than the control muffins, showing lower
lightness values, both in the crust and in the crumb. A significant effect of the amount of the red
grape pomace product was found; thus, the higher percentage of added RP, the lower lightness in the
crust and crumb of muffins. The results obtained in terms of lightness were similar to those found by
Walker et al. [28] and Bender et al. [25] when fortifying muffins with products obtained from red and
white grape-skins.
Table 4. Colour parameters (mean ± standard deviation) of the control (C) and the reformulated muffins.
Sample L* a* b*
C 35.6 ± 1.3 d 12.4 ± 0.2 e 20.1 ± 2.2 e
WP-10 33.2 ± 2.2 c 10.7 ± 0.4 d 15.5 ± 1.4 d
Crust WP-20 31.8 ± 3.0 c 9.76 ± 0.37 c 14.2 ± 1.3 c
RP-10 29.0 ± 4.0 b 7.10 ± 0.62 b 10.8 ± 0.5 b
RP-20 26.3 ± 4.1 a 4.30 ± 0.15 a 6.14 ± 0.02 a
C 45.4 ± 3.2 d 6.19 ± 0.29 c 18.9 ± 0.7 d
WP-10 37.3 ± 0.8 c 6.87 ± 0.36 d 14.9 ± 1.0 c
Crumb WP-20 35.6 ± 3.9 bc 7.24 ± 0.74 d 14.6 ± 1.6 c
RP-10 33.9 ± 2.6 b 4.22 ± 0.21 b 8.85 ± 0.68 b
RP-20 29.7 ± 4.8 a 3.83 ± 0.47 a 5.79 ± 1.25 a
WP-10: muffin with 10% of white product (WP); WP-20: muffin with 20% of WP; RP-10: muffin with 10% of red
product (RP); RP-20: muffin with 20% of RP. Different letters in the same column denote significant differences
between products. LSD test and p-value < 0.05.
As with lightness, an effect of the type and the concentration of the product employed in the
parameters a* and b* was found. Thus, the lowest values of these parameters corresponded to the
muffins with red grape pomace product. In relation to the a* parameter in crumbs, the muffins with the
product obtained from white grape pomace showed higher values of this parameter than the control
ones. This may be due to the own colour of the white grape pomace product.
As can be seen, the combination of the a* and b* parameters gave as a result the colour of each
sample. The a* and b* values obtained in the samples with white product were higher than those with
Foods 2019, 8, 419 7 of 15
the red product, giving the characteristic yellowness of whole-wheat muffins. However, lower values
were related to the dark colour of the muffins with red product. The same tendency towards lower
values of the a* and b* parameters was observed by Acun and Gül [15] in the preparation of biscuits
with red grape-skin pomace extracts.
3.4. Texture
The addition of the two concentrations of products also modified the texture parameters evaluated
(Table 5). The incorporation of the seasonings increased significantly (p < 0.05) the hardness of the
muffins, which may be due to the higher fibre content of these samples, which increased the dough
density and reduced the incorporation of air during baking. Thus, the resulting crumbs were more
compact, which increased the force required to compress them [25]. In the case of the muffins with WP,
it was also observed a relationship between the amount of added product and the hardness.
Table 5. Texture parameters (mean ± standard deviation) of the control (C) and the reformulated muffins.
WP-20 and RP-20 muffins also underwent a significant reduction (p < 0.05) of springiness due to
the higher levels of fibre, which increased the density of the samples, reducing their springiness [28].
Fibre also influenced on cohesiveness; thus, the values decreased as the percentage of grape pomace
product added increased. The desegregation effect of the fibre is reflected in lower requirement of
energy when TPA performs a second compression [30].
In relation to chewiness, no statistically significant differences were found among the muffins
with grape pomace products, although their values were higher than those of the control muffin.
As for the resilience, an effect of the percentage of seasoning added was found, decreasing the
resilience value as the added amount of seasoning increased. All the samples studied showed low
resilience values (0.218 ± 0.022), which are typical values of recipes with fat and sugar [25].
The results of the textural instrumental technique in the present study (Table 5) were similar to
those found in the study by Walker et al. [28], in which the impact of the incorporation of red and white
grape-skin in different products was evaluated with the aim of obtaining functional bakery dough.
The same tendency was observed in the study performed by Bender et al. [25] on muffins.
Figure 1. Mean consumer liking scores of the control (C) and reformulated muffins (n = 104). WP-10:
muffin with 10% of white product (WP); WP-20: muffin with 20% of WP; RP-10: muffin with 10% of red
product (RP); RP-20: muffin with 20% of RP. Different letters in the bars denote significant differences
between products. LSD test and p-value < 0.05.
The results obtained in the sensory evaluation of the present study are similar to those obtained
by Bender et al. [25]. However, Pitre et al. [31] reported an acceptability level of 67% without
prompting any observation by the consumers for a biscuit prepared with 10% of grape-skin extract,
and Mildner-Szkudlarz et al. [32] observed no significant differences in the acceptability of muffins
added with less than 20% of white grape-skin extract.
As previously reported, the muffins elaborated with the grape pomace products could be labelled
with the claim “high in fibre”. However, consumers’ main scepticism regarding functional foods
resides in the veracity of health claims [33]. Thus, in order to obtain product acceptability, clear,
understandable and verified information has to be communicated to the consumer [1]. Therefore, it
would be needed to further investigate the willingness to purchase of consumers when the claim and
information about healthy properties of the new muffins (with WP or RP) are provided to consumers,
and thus, to know if the reduction of 0.76 points in the overall-liking scores of the WP-10 and RP-10
muffins could be assumed.
Figure 2. Just-about-right (JAR) scale percentages of responses grouped in three levels of the muffins
(n = 104). (a) C: Control; (b) WP-10: muffin with 10% of white product (WP); (c) WP-20: muffin with
20% of WP; (d) RP-10: muffin with 10% of red product (RP); (e) RP-20: muffin with 20% of RP.
In the control muffins all the parameters were mainly scored in “JAR” category, ranging from 53%
(sponginess) to 76% (hardness). The most remarkable results were that 33% of the consumers thought
that the sponginess of the control muffins was “much more” and 28% indicated that the crumb colour
was “much less”. The reformulated muffins showed lower percentages of JAR responses than the
control muffins for most of the attributes evaluated (see Appendix A), and it was observed that the
percentage of JAR responses dropped as the percentage of addition of the grape product increased.
The hardness was the parameter that showed lower differences with control muffins, especially
when only 10% of grape pomace product was used. However, larger differences in scores were
observed related to colour attributes, especially in muffins elaborated with the product from red grapes.
In these cases, responses in the group “JAR” were lower than 25% in the RP-10 muffins, whereas in the
RP-20 muffins, these percentages dropped to the 2%. Consumers considered these muffins to have
“much more” colour. Furthermore, they also indicated that they had “much more” flavour (58%).
The obtained results also showed that the white grape pomace product decreased the perception of
sweetness with respect to the control muffin more than the red grape pomace product. Thus, in the
WP-20 muffins, the percentage of responses “much less” was 23 points higher than that of the control
muffins, whereas in the RP-10 muffins, this increase was only of 10 points.
An analysis of penalizations was carried out to understand which of the attributes under evaluation
affected the acceptability of the product to a greater or a lesser extent. The penalizations indicate how
much the global acceptability of a product drops when a particular attribute is seen as “much more” or
“much less”, in such a way that the higher the values that are obtained, the greater the impact of the
aforementioned acceptability [24]. The results are shown in Table 6.
Foods 2019, 8, 419 10 of 15
Table 6. Penalty analysis of the control (C) and the reformulated muffins.
Table 6. Cont.
In those categories where the number of responses was below the threshold of 20%, the
penalizations were not taken into account [34]. Moreover, an attribute was only considered to
affect the acceptability of the product when the mean drop was higher than 1 [24].
Neither of the parameters had mean drops over 1 (data not shown), which means that there
was no attribute that by itself affected the acceptability in a significant way. Then, results seemed to
point out that the acceptability depended on the joint perception of all of them together. However,
penalties could not be calculated for the colour of the crust and the crumb of the muffins elaborated
with red grape pomace product; a high percentage of responses were obtained at the extreme point of
“much more” on the scale: over 90% for a concentration of 20% seasoning. This fact highlighted that
colour was one of the parameters furthest from the “ideal colour”. However, there was not a clear
correlation of this fact with the overall-liking of the muffins, especially with those elaborated with red
grape pomace product. Previous studies noted that muffins’ colour is a decisive factor in the global
acceptability as well as in the purchase intention [35], however, the obtained results are not as robust.
Thus, 88% of the consumers indicated that the acceptability of the crumb colour of the RP-10 muffins
was "much more", whereas for the control muffins this percentage was 13%. However, the acceptability
of RP-10 muffins was only 0.76 point lower than that of control muffins. This fact points out one of the
problems of the JAR scales, which is that this methodology evaluates the degree of acceptability of
each attribute in an individual way, without taking into account that the modification of an attribute
can influence the perceptions of others in either a positive or in a negative manner [24].
Independently of previous comments, if the dark colour of the muffins elaborated with grape
pomace product was actually an important problem for the consumer acceptability of the product,
a possible solution could be to inform the consumers of the origin of the “dark colour”, explaining
also the associated healthy effects of their components such as fibre, antioxidants (grapes phenolic
compounds), minerals etc. In fact, consumers usually associated darker baked products with healthy
properties and higher fibre and whole grain content [28]. In this way, if the new muffins were marketed
specifying the healthy characteristics of the grape pomace product, the dark colour of the muffins
elaborated with them should not be a reason for their initial rejection by consumers. Other studies have
shown that providing information on the fibre content of muffins has a positive impact on consumer
perception and acceptability, regardless of the appearance of the product [11].
4. Conclusions
The incorporation of grape pomace led to changes in the colour and textural properties of the
new whole-wheat muffins with high content of fibre. However, the sensory analysis indicated a good
level of acceptability for these new healthy whole-wheat muffins with the grape pomace products,
although consumers indicated that some sensorial parameters were different from those considered as
“ideal”. Moreover, the muffins with 10% of WP or RP had higher liking scores than those with 20%
of WP or RP. However, in spite of the fact that a penalty analysis indicated that the darker surface
colour in muffins with RP could affect the acceptability, the advice of the use of RP in the formulation
and its healthy properties could overcome this problem. Thus, further studies would be needed in
which information on health properties is presented to the consumers during the evaluation to fully
understand the willingness to purchase of new healthier products. Furthermore, the information
obtained from the JAR scales and penalty analysis indicated that grape pomace products obtained
from red and white grapes at a level of 10% could be a good alternative to develop new healthier
muffins with high fibre content.
Author Contributions: M.O.-H. and I.G. were responsible for conceptualization, formal analysis, and methodology.
S.d.P.-A. analysed the samples and the data. M.O.-H. and I.G. wrote, reviewed and edited the draft manuscript.
M.L.G.-S. was responsible for project administration and reviewed and edited the draft manuscript. All authors
read and approved the final manuscript.
Funding: This research was funded by the Authonomous Government of Castilla y León (BU282U13).
Conflicts of Interest: The authors declare no conflict of interest.
Foods 2019, 8, 419 13 of 15
Appendix A
Figure A1. JAR scale percentages of responses grouped in three levels of the control (C) and the
reformulated muffins for each attribute (n = 104). WP-10: muffin with 10% of white product (WP);
WP-20: muffin with 20% of WP; RP-10: muffin with 10% of red product (RP); RP-20: muffin with 20%
of RP. Parameters evaluated: (a) surface colour; (b): crumb colour; (c): sweetness; (d): hardness; (e):
sponginess; (f): flavour.
Foods 2019, 8, 419 14 of 15
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