Cross-Specialization Training of Grades 7-10 Science Teachers

on their non-major Science Subject

October 15-20, 2018
Peque, Samantha Nicole Kate P. 10 – Nebres 3/22/2022

Grade 10 Activity Sheet on Protein Synthesis Activity 3: Decoding DNA segment

Objective
Apply the concept of protein synthesis through the amino acids coded for the mRNA
codon using the genetic code.

Materials
Pen
Crayons
Activity sheet: Decoding DNA segment

Procedure
Study the given scenario (see attached activity) and study the profile of the suspects
and additional information.
Using your knowledge about DNA and RNA base-pairing, decode the DNA sample in
order to identify the sequence of amino acids. Use the amino acid identifier chart as
your reference.
Compare the sequence of amino acids (proteins) to the profile of the suspects and
additional information given in order to determine the corresponding traits.
Name the culprit using the data or information you got.
Using the pencil and crayons make a cartographic sketch of the suspect.

Q1. What amino acids sequences did you get upon decoding the genes?
• nose hair: Phenylalanine – Lysine
• shape of eyes: Valine – Alanine – Valine
• lips : Phenylalanine – Glutamic Acid – Alanine
• ear attachment: Threonine – Tyrosine – Serine
• type of hair: Glutamic Acid – Phenylalanine
Q2. What are the traits of the culprit based on your data?
• The traits of the culprit based on my data were short nose hair,
chinky eyes, full lips, free earlobe, and curly hair.

Q3. Who among the suspects matches with the sequence of amino acids (proteins)
you got?
• Among the three suspects, suspect number two, Von Go, matches
the sequence of amino acids that I have.

Q4. Draw the possible appearance of the culprit.

Adapted from the presentation of Dr. Catherine Genievie Lagunzad, during the ACED Training of Public School Teachers
 Cross-Specialization Training of Grades 7-10 Science Teachers
on their non-major Science Subject
October 15-20, 2018

Decoding DNA Segment

An intruder razed the science laboratory of a Junior High School. The school is in
chaos. The school administrators and teachers demanded to know the culprit. They
have to identify the culprit and do it fast. They had three suspects namely Rudy Tee,
Von Go and Rambo Tan. As a forensic expert, your mission is to identify the intruder
using the profile of the suspects and other information taken from the laboratory:

THE PROFILE OF THE SUSPECTS

Rudy Tee • full lips
• Flunked physics class three • attached ear lobe
times • straight hair
• with long nose hair,
• oval eyes

Von Go
• His investigative project is • short nose hair
about the potential • chinky eyes
applications of nuclear fusion • full lips
in generating energy. • free earlobe
• curly hair

Rambo Tan
• Suspended for a week by the Disciplinary Committee for repeated tardiness.
• short nose hair
• oval eyes
• harelip
• attached earlobe
• wavy hair

Adapted from the presentation of Dr. Catherine Genievie Lagunzad, during the ACED Training of Public School Teachers
 Cross-Specialization Training of Grades 7-10 Science Teachers
on their non-major Science Subject
October 15-20, 2018

Additional Information: Below are the specific amino acid sequence for specific trait.
1. Nose hair
• Short : phe-lys
• Long : leu-lys
2. Shape of Eyes
• oval : val-ala-ala
• chinky : val-ala-val
3. Lips
• Full lips: phe- glu-ala
• Harelip:phe-lys-ala
4. Ear attachment
• Free earlobe : thre- tyr-ser
• Attached : leu-arg-gly
5. Type of hair
• Straight : lys-glu
• Wavy : phe-ala
• Curly : glu-phe

THE DNA CODE OF THE SUSPECT

A fresh DNA sample was obtained from the laboratory; believe to have been left by
the suspect. Using the DNA sequencer the following nucleotide sequence was
obtained:
SUSPECT: TAC-AAA-TTT-ATC-TAC-AAA-CTT-CGT-ATC-TAC-CAT-CGT-CAT-ATT-TAC-
TGG- ATA-TCG-ATC-TAC-CTT-AAA-ATC
Complementary base pairs: DNA is represented by nitrogen bases that are read in
groups of threes. To decode, use the DNA as a blueprint to produce mRNA. Then use
the mRNA as blueprint to produce the linear sequence of tRNA. A specific nitrogen
base in the DNA pairs up with a specific nitrogen base in the mRNA, and a specific
nitrogen base in the mRNA pairs up with a specific nitrogen base in the tRNA.
Breaking the Code: Complete the data table below.

DNA triplet code mRNA tRNA Amino acid

TAC AUG UAC START CODON (MET)

AAA UUU AAA PHE

TTT AAA UUU LYS

ATC UAG AUC STOP CONDON

3
 Cross-Specialization Training of Grades 7-10 Science Teachers
on their non-major Science Subject
October 15-20, 2018

TAC AUG UAC START CODON (MET)

AAA UUU AAA PHY

CTT GAA CUU GLU

CGT GCA CGU ALA

ATC UAG AUC STOP CODON

TAC AUG UAC START CODON (MET)

CAT GUA CAU VAL

CGT GCA CGU ALA

CAT GUA CAU VAL

ATT UAA AUU STOP CODON

TAC AUG UAC START CODON (MET)

TGG ACC UGG THRE

ATA UAU AUA TYR

TCG AGC UCG SER

ATC UAG AUC STOP CODON

TAC AUG UAC START CODON (MET)

CTT GAA CUU GLU

AAA UUU AAA PHE

ATC UAG AUC STOP CODON

Characteristics and traits of the suspect based on the DNA code.

Nose hair: Short
Eyes: Chinky
Lips: Full lips
Ear attachment: Free earlobe
Type of hair : Curly

Name and cartographic sketch of the suspect

VON GO