GABORONE SENIOR SECONDARY SCHOOL

STUDENT NAME

CLASS

BIOLOGY FORM 5
PAPER 5 Alternative to Practical END OF TERM 1 2022
DURATION: 1 HR

READ THESE INSTRUCTIONS FIRST

Answer all questions

Write your answers in the spaces provided on the question paper.

You may use a calculator.

Use a sharp pencil for your drawings. Coloured pencils or crayons should not be used .

Any rough working should be done in this booklet.

Do not use any staples, paper clips, highlighters, glue or correction fluid.

The number of marks is given in brackets [ ] at the end of each question or part question.

FOR EXAMINER’S USE

Question 1

Question 2

TOTAL

1
1. Fig .1.1 shows photographs of sections through two fruits. Fig 1.1a is a pea pod while Fig 1.1b are
groundnuts.

FIG 1.1a FIG 1.1b

(a) (i) Make a large drawing of the section through the pea pod in Fig 1.1a.

[5]

2
 (ii) Measure and record the length of the pea pod in Fig 1.1a.

Measure and record the length of your drawing.

length of Fig 1.1a …………………………………………………………………………………………………………….[1]

length of your drawing …………………………….………….………………………………………………………….[1]

(iii) Calculate the magnification of your drawing.

Show your working

Magnification……………….……………………………..………… [3]

(b) Give one observable similarity and two observable differences between the fruits in Fig 1.1a and
Fig1.1b

Similarity …………………………………………………………………………………………………………………………………………

…………………………………………………………………………………………………………………………………………………….[1]

Differences

Fig 1.1a Fig 1.1

1.

2.

[2]
(c) In an experiment, a student used water to make a solution of crushed groundnut.
She added 5 drops of sodium hydroxide solution.
She then added 5 drops of copper(ii) sulphate solution and gently swirled to mix.
She observed that the mixture turned from blue to violet.

State what conclusion she could make from her observation.

………………………………………………………………………………………………………………………..…………………………

…………………………………………………………………………………………………………………………………………………… [1]

3
(d) (i) Describe how you would test the groundnuts to see if they contain fats.

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………………………………………………………………………………………………………………………….……………………….[4]

(ii) State the result of the test if fats are present.

………………………………………………………………………………………….…………………………..………………………..[1]

(iii) Groundnuts contain starch.

Describe what you would observe if they were stained with iodine solution.

…………………………………………………………………………………………………………………………………………………[1]

[TOTAL 20]

4
2. Catalase is an enzyme found in most living organisms. It catalyses the breakdown of hydrogen
peroxide to water and oxygen.

Potato tissue contains catalase. When a thin disc of potato is dropped into a test tube
containing hydrogen peroxide solution, it first sinks to the bottom. As oxygen is produced ,
bubbles form on the potato disc that make it float to the surface.

Some students did an experiment to find the activity of potato catalase on different
concentrations of hydrogen peroxide solution.

1 mm thick discs were cut from the end of a cylinder of potato tissue as shown in the fig 2.1.

FIG 2.1

One disc was dropped into a test tube containing 15 cm3 of 1% hydrogen peroxide solution. A
stopwatch was started immediately. The time taken for the disc to reach the surface of the
solution was recorded. The time was rounded to the nearest whole second.

A second disc was then dropped into the test tube and the time taken for it to reach the surface
was recorded. This process was repeated with a third disc.

The experiment was then repeated with a test tube containing 15cm3of 2% hydrogen peroxide
solution.

The results are shown in table 2.1.

The procedure was repeated using a test tube containing 15cm3 of 3% hydrogen peroxide
solution.

The times recorded were 8.3, 6.9 and 9.1 seconds

5
(a) (i) Complete the table by entering the results for 3% hydrogen peroxide solution (rounded to the
nearest whole second) and calculate the mean.

TABLE 2.1

[4]

(ii) Describe the effect of increasing the concentration of hydrogen peroxide solution on the time
taken for the potato disc to float.

…………………………………………………………………………………………………………………………………………………………

……………………………………………………………………………………………………………………………………………….…….[1]

(iii) Suggest what the students could do to make their results more reliable

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………………………………………………………………………………………………………………………………………………………[1]

(iv) Suggest one possible source of error in the method used in the experiment. Explain why this
could have affected the results.

Source of error………………………………………………………………………………………………………………………………

Explanation…………………………………………………………………………………………………………………………………….

…………………………………………………………………………………………………………………………………………………..[2]

(v) Another Potato disc was dropped into a test tube containing 15 cm3 of distilled water. It sank to
the bottom of the test tube.

After 30 minutes, it was still at the bottom of the test tube.

Explain why the disc did not float

………………………………………………………………………………………………………………………………………………………..

………………………………………………………………………………………………………………………………………………………..

………………………………………………………………………………………………………………………………………………………[2]

6
(b) Explain in detail how you could show that it was an enzyme that caused bubbles to be produced
when the potato discs were dropped into hydrogen peroxide solution.

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………………………………………………………………………………………………………………………………………………………[4]

(c) A group of students decided to investigate the effect of increasing the concentration of catalase on
the rate of oxygen production. They altered the concentration of the enzyme by using different
numbers of 1mm thick potato discs.

They used the apparatus in FIG 2.2 below to collect the oxygen produced by different numbers of
potato discs in 30 cm3 of 3% hydrogen peroxide solution. They recorded how long it took to
produce 5cm3 of oxygen with each number of disc.

FIG 2.2

Their results are shown table 2.2.

TABLE 2.2

7
(i) Construct a line graph of the data in the table on the grid below, draw a smooth curve through
your points.

(4)

(ii) Describe the effect of increasing enzyme concentration on the rate of the reaction.

……………………………………………………………………………………………………………………………………………..

……………………………………………………………………………………………………………………………………………….[1]

(iii) Suggest an explanation for the shape of the graph between 4 and 5 discs of potato.

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………………………………………………………………………………………………………………………………………………[1]

[TOTAL 20]