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BAR PROM). lb ks BEE A EAL » 2006. C7) Bei A. ETE ABE ELM). ke = TE ACE tH RAL «2007, [8] Sambrook J,Russell DW. 4F 38 404i rig M]. 3 A. SUR Me. E HEL AR J HR HE. ACS BEE AEA 2002. [9] Richard J R. Analysis of Genes and Genomes{M], Chichester; John Wiley8.Sons, Ltd, 2003. 7CARB) AEAEPBAARLA PHM HGRA, MR He A om LDNA HARD DNA BG Bite 15 Mh SEP PAR EE A aR FY bap fo DNA 8 dR Bb A AF ER 0 9h fe RI de DNA AF Hin GEAR OE AEA fo AAT EE. Rh DNA 38 i 40 45 4 ii vk BS fle LIL OY TAR He RP 84 AED TBR OPK DNA TIAL ER AS «BD OF A He J BF) BA FL OE AY. AE DNA BEAL it REAP PE KS UA Bh a IL BR AE 0 BY DNA Ff RAT FAL BR SEA EAR HBB) RMA TA BY DNA Hr BEER DNA FR ABD AR ev LK) DNA Ar Bx He WU Hh PL He aR GY PE Be CAGE DNA GE BEE DNA We tH BD » A AR BI AY HEA DNA. EAE DER AIRE 9 LA SBE LEAR SD RE DNA EAT 4d SALA BA AN i Ep RE SL AEE HG OG ES FD TEAR PERE DET EASE RL. SE DT. AER Bb Ba $m A A) TE» 0 dB ES EE A A 1h FEL 9 bth 3) A TT PE KR BR AE A OR V1 BA. 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EcoB 5'‘TGA* NNNNNNNNT GCT 3'-ACT MMMMMMMM A CGA p= # 1000 bp bya82% EALTEHIRS ea 1 OM A ae HA DNA. FS I) PA AS TE A TE HANK 2.) TL 7 il 2 ‘AL AE FaSEL ) T A d e S E > G He AS MB EH eth 2 HR SAL) BE VA ESL Py «AT ERA AE TE» BP Me Ek ‘i «RM OU AS CPO PE UL POLE ES AN PE A» BR PE PD A WAR ZHAO SE SG. APA THESE AT Mg?’ SATP SAM 45 By BS 5 1 BA fic he PE (928 PAE fe « ELIE PE « FG IA 09 EBS AAG «AS dT 9 A ARIA EE RS .7~26 bp ARF. MM Mbo TAS FESUE 5'AAGA3' HOUTA IME 5'SGAAGANNNNNNNN 4 N-3! 3'-CTTCTNNNNNNN { NN-5' PEEL — ARE H DE E AC «PLAT AS 9 EE OL SB 8 a — FP AL Bd PE a AE DNA Hr BS BE ach» PAT 1 EP eH Ak >A 3) TD SU BRR ll PR A 1970 4H. O, Smith 74 JG Min ARMM ORT Rl pi RD WES HH TT 200 i ER A. TT WET AT CBM A ATS 93.6. UD UI Mig?” 9 LE AB aE EE «A fH FG SAL, ML 260 FG AS (EL AE A FP AES DNA FF 3 TE PEASE EP) RS AT Pk PS A Oy DNA Hit Be AR PS HG FD TEL SEE EAST” RE AE Ae RB 4, BRL al RP A O19 TY 2.1, 2 RHE ABE PY EY cit EEL SA ROA A 50H DR i 2 2S 4 OF HB — 9 i I SARA LR. 1973 4. H.O. Smith Al D. Nathans 48 Ht (9 VBE aa He FA AEBS ie I PLB OEP PITTS CATT SE BE FA A 19 RE A OD, — BR A ~S SEL BBR SE KG RHR HT AAR: I FS — PE SANS BHR OK FTE PA: ON I FE Be FE ARIEL EAS 2 5 LOR PA 5 AS TE Bea th PIM a A ak th RH — PR + TE sr BR tad 55 A Wh Bae L SG | BY « UE Ae BB ML RS SE eA A A A BA — Td SE i SUAY A Ud BR il AS AS FR» VTE AR Py Be S28 os EI TE 9 FJ UI. A Hind | AOA CE Tht RF LAF HH Haemophilus influenzae ) (i) Ry th BA EW BER AY — BE il PE YB. EcoR | JEtE KG EPH (Escherichia coli) A) Hi 25 YE R BOR BBE ADS — FP BR tl CE ABA. 2.1.3 TL CURE HE eA BD EY EAE $5 1 RY Be TM 7 Bik i A AN GAS Td TL 7 ie AS — AL WAR TE PEE. HEARS AT PD BR HE 1, BRA AR AE FE DEY KBE Dik il A FP BPE REINA EO PRET TP A FO aLBALE 422 55S SHES HT VA VA > 3 ae tid FY PLE INA. ie 28 ais 5 AS TL 1 HE RP SHEL BOI FF) AEF BY FA ~ 8 SE LAG EB 4 ARIE A Sau3A | GATC 5 EDULE :Mae Ill GTNAC (N Jy ff RIE) EcoR [| CCWGG CW 3 A ak T) 6 TORI: EcoR | GAATTC Hind tll AAGCTT BamH | GGATCC 7 APR EABILI SE : BooC T ~CCTCAGC 8 ARE I GcGGccGc D> BC al HC A ET DA be eA A, ind TD BS IL CTPyPuAC, Sef Py 99 C&T, Puy A at GikkE Hind [REI 4 Ae TE FEA 22 BG WL A) FT BG 9 PE Lk «A ASHI NA SbF BS EF BS «BE 1S BMY) Hr BEDE TEA 7ST) BS BR i PR 1) ee — A St BP AS TD 1) EE EL A AY PARA UI AK BE. RDNA SF GC AY Hy 5016 «i EL DO SE A A i J BBL ZARA EE SEH IE AE me ASME 0) SF 3 BEE 4 AER » SE ARE HE BIE AS EF BALL» HG LAS Tl Ba: Hak ASV, DE, 08) AB BAST 207 FF AB 2 RR a A FF 9 AD AC ey 180° Be HE AE A HY [9d 3X 44 #4 Cpalindromic structure). 7£— SRC iH 5 SC Si Hy A MLA. AB A HY AF. step on no pets” BEIE— FP FAL CfA TE (BS A BRE BRE. NA. fy ad 3 2A gH A SE J Le AL OM 5) EF BRE EA» — 2 ae of a TT FA) EL eA wee 5”) 3" Ad Co BG ae Ds A Zee eB «FT BA AE ZED FH — AHEM 180° TG — ARE AE. EcoR 1 UW FR ASE EMEA TTA LL DNA M5! 58) 39 EFF A ERE A AE HE MOE ABE: GAATTC, Xb Fp fi FAVS DS {ie IE ZT) SH A Aa EA HLA A 2, wp Bt RAE FE 1) Bat fie DNA 4b FBR il PR A 1 9 PE PAD CE A _ — T A BOWE ak Fa” VBR" eras PER hd He BR A HE LE DNA: 9) 19 AD HB ETA R [Gy AATTC-3') Ban [I (S'-AT ¥ CGAT-3') Sma 1 (5'-CCC ¥ GGG-3'), Pst STGCA 4 G-3!) Fs AF A BBCI AL FR SF 3 PA, I Sau3A | (S'-¥ GATC-3'), Nia I(5'-CATG 4 -3'),EcoR I] G'- ¥ CCAGG-3') 4. 2) A A AR DNA 5p -F 2 BRR al HE A A EB a 7A) DNA Hr BRR Si LT82H SALAHLAB CL) BB PEAR Bits) DBE DNA. i ie YOO EJ CE LS FBP — PS ETA TANYA DNA Ae Satay 5! Se tH a 3'9e He AY DNA a a EAP EAA Td 9 5 td A 11) AF AT AR Bab DNAS} 408 25 5 LEAS TT "LE — a EL ZB A (cohesive end) a DE fai38 (matched end). 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Saw3A T (5'- y GATC-3") Al Mob I (5'- ¥ GATC- 3°), Bam H | (5'-G 4 GATCC-3') ll Bse I (5'-G ¥ GATCC-3') , Hind [ll (5'-GTY 4 RAC-3') 45 Hine I] (5'-GTY ¥ RAC-3)(Y=C af T.R=A Be G) 5X28 PRG I TAF C2) BUSH PFU ALA] AL AB Ve, K pn 1 (5'-GGTAC ¥ C-3') Al Ace65 1 (5'-G ¥ GTACC-3"),Aar I] (5'-GACGT ¥ C-3') Ml Zra_T (5'-GAC ¥ GTC-3'), Sma T (5'-CCC ¥ GGG-3") Al Xma_ 1 (5'-C } CCGGG-3") LASHER I FEE. 3) 5B Ay BE ABS MG If FF BLE) A FB TP — Ri AL 2 «fe Apo 1 (5'-Ry AATTY-3')(R=G a A,Y=C ak T) BAPE WIM 9'-G Y AATTC-3" 5 Ay AATTC-3',5'-G ¥ AATTT-3',5'-A ¥ AATTT-3' TUF RA Ih] EcoR 1 (5'-G ¥ AATTC- 3"), tht Apo 1&1 EcoR AY DIAE x28 BRR I ll Dy & Or. 4) Fel Fe ii i a 1 0 PE 5 RAS Fd 5 tS Dd ELS A 7 EAS Sti ARN ADEN Cisocaudarner) , GA FAHY ML AME BamH 1 (5'-G ¥ GATCC-3") Bel 1 (5'- Ay GATCT-3') Bgl T.S'-T ¥ GATCA-3") ih AB 244 FLFR BE 5 Ee 0D AE FAT I A AB ed ELE Hel A PUGLEY DNA Jer PEAT 5'-GATC Bite ARS. ME MAT 5 UL i HE Bade HY DNA Pr Bai 3 Bi HE AC Sith ZT 09 A FT Oe Be ik ZEAE Te BS OP Piste Rj BE PAE AB» ok — TF BB SG Ae A A ARR BeAiL a Chybrid site) . XARA EAE Ak — AB AE AS (AB FE AB 0) CE 0] — TdF A (EL tn 9 BIER BAS A Sau3A 1 (5'-¥ GATCC-3') Al BamH | (AE BSTE RAY AREAL Rt Sau3A IT VaR Se RUB I MAE RAE BamH [ARR CAI 2-2). 23