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Lab# Paper Chromatography

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19 views5 pages

Lab# Paper Chromatography

Uploaded by

Unique McDonald
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as PDF, TXT or read online on Scribd
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North Georgetown Secondary School

Grade 10 Science/ Tech

LAB # 3

Date: 2024-06-06

Topic: Separating Techniques (Paper Chromatography)

Aim: To determine the dyes found in black ink by paper chromatography

Skill Assessed: Observation, Reporting and Recording and Analysis and interpretation

APPARATUS/MATERIALS:

1. 1 pencil
2. 1 glass rod
3. 1 ruler, scissors
4. 1 pieces of chromatography paper(filter paper as a substitute)
5. 7 cm3 of alcohol (rubbing alcohol)
6. 25 cm3 measuring cylinder
7. 1 100 cm3 beakers
8. Tape
9. Black ink (permanent marker)

Method/ procedure

1. *-Cut one strips of chromatography paper with the dimensions of 9 cm x 1.5 cm.
2. On one strip, mark a line 1.5 cm away from one end and place a dot of one permanent marker ink
in the centre of the line.
3. Measure out 7 cm3 of alcohol and pour it into the beaker.
4. Tape the unmarked end of each chromatography paper to a stirring rod and gently place the
chromatography paper into the water. Make sure that the dot does not touch the water and then
allow the experiment to run until the dyes are separated. Mark the furthest point on the paper the
ethanol rises.
5. Allow the paper to dry and then mark the points at which all the dyes separated. Label each
separated component using letters, starting with A being the ink component which separated
closest to starting point.
6. Make observation
7. Calculate the Rf value: - Rf value describes how soluble a substance is. Rf = distance travelled
by spot or component /distance travelled by solvent)
Diagram

Observation/Results

Calculations

Distance moved by the alcohol (solvent)

Distance moved by blue dye (component)

Distance moved by red/pink dye (component)

Distance moved by yellow dye (component)

Rf of each component (distance travelled by spot or component / distance travelled by solvent)

Table of Results

Component of black ink(dyes) Distance moved Rf value

Table showing the dyes found in black ink and their Rf values
Discussion

1. Define paper chromatography and describe how paper chromatography is used as a separation
technique.
2. Explain the process of chromatography.
3. Which dye is most soluble and why?
4. Define Rf value and explain one of its application.
5. Outline how paper chromatography is useful in this particular experiment.

Assumption- at least one

Precaution- at least one

Source of error- at least one

Conclusion
Marking scheme

Skill Assessed: ORR

Criteria Maximum marks Marks awarded

1. For making at least three(3)


observations 3
2. Resulting chromatogram is shown 1
3. Recording distance travelled by dyes 3
4. Logical sequence of report
a) 3 headings 1
b) 3 and more headings 3

Total 10

Skill Assessed: Manipulation and Measurement (MM)

Criteria Maximum marks Marks awarded


Measurement
Preparation of chromatography
paper
1. Baseline drawn with 1
pencil 1.5/2cm from one
end of paper
2. Paper suspended so that
baseline is above solvent 1
Manipulation
Measurement of chromatogram

1. Line at solvent front and


top of separated dyes 2
2. Distance from origin to
solvent front 2
3. Distance from origin to blue
dye 2
4. Distance from origin to pink
dye 2
Total 10
Skill Assessed: Analysis and Interpretation

Criteria Maximum marks Marks awarded


1. For calculating Rf values
of the dyes 3
2. Defining
chromatography 2
3. Explaining the process of
chromatography 5
4. Stating the dyes present 3
5. Stating which dye is
most soluble and why 2
6. Defining Rf value 1
6. Outlining how paper
chromatography is useful
in this particular 2
experiment.
7. Stating one source of
error 1
8. Writing a conclusion
related to the aim 1
Total 20

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