Synthesis of indolo[3,2-b]carbazole-based new colorimetric receptor for anions: A unique color change for fluoride ions

Ajit Kumar Mahapatra*, Giridhari Hazra and Prithidipa Sahoo

Preliminary Communication
Address: Department of Chemistry, Bengal Engineering and Science University, Shibpur, Howrah 711103, India, Tel.: +91 33 2668 4561; Fax: +91 33 2668 4564 Email: Ajit Kumar Mahapatra* - [email protected] * Corresponding author Keywords: anion binding; colorimetry; fluorescence quenching; fluoride binding; indolocarbazole 2010 Mahapatra et al; licensee Beilstein-Institut. License and terms: see end of document. Beilstein Journal of Organic Chemistry 2010, 6, No. 12. doi:10.3762/bjoc.6.12 Received: 01 October 2009 Accepted: 30 January 2010 Published: 08 February 2010 Associate Editor: P. Skabara

Open Access

Abstract
A novel indolocarbazole-based chemosensor 1 containing hydrogen bond donor moieties has been established as a selective colorimetric and fluorometric sensor for F in CH3CN/H2O (4:1 v/v). Upon the addition of a series of tetrabutylammonium salts to receptor 1 in aqueous CH3CN, only when the counter ion was F was a significant color change (from light violet to dark orange) observed.

Introduction
The design and synthesis of chromogenic receptors for biologically important and environmentally harmful anion pollutants has attracted considerable attention in supramolecular chemistry [1,2]. Most of the synthetic chemosensors generally involve covalent linking of an optical-signaling chromophoric fragment to a neutral anion receptor containing urea [3], thiourea [4], amide [5], phenol [6,7], or pyrrole [8] subunits, which can provide one or more H-bond donor sites for selective binding and sensing of certain anions, especially F, AcO, H2PO4, etc. In particular, the selective sensing of fluoride has gained attention due to its significant role in clinical treatments e.g. dental care [9], osteoporosis [10] and for the detection of fluoride in bones as a result of over-accumulation [11]. This diversity of function, both beneficial and otherwise, makes the problem of fluoride ion detection of considerable interest. In this context, a colorimetric chemosensor is of particular interest due to its simplicity. Color changes that can be detected by the naked eye are widely used as signals for detection of anions without the need for any expensive equipment or even without the requirement of any equipment whatsoever [12,13]. In the last few years, although some synthetic receptors have become available for fluoride ions [14-28], there is a paucity of reports on selective naked-eye chemosensors for fluoride [29-31]. Nitrophenyl, nitronaphthalene urea [32-34], naphthalene triphenyl-phosphonium [35], benzimidazolyl pyridine
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[36,37] and oxidized bis(indolyl)methane [38] as signal units for fluoride have been reported as chromogenic chemosensors, but a indolocarbazole ligand for the anion remains to be developed. Recently, Bhardwaj et al. reported a tripodal receptor [39] bearing catechol groups [40] for the chromogenic sensing of fluoride ions. Numerous bis(indolyl)methanes and their derivatives exhibit important biological activities [41]. Therefore, there has been great interest in the synthesis of bisindole compounds both naturally occurring and totally synthetic. As an extension of our work [42] on supramolecular chemistry, we now report a simple and new indolocarbazole-based molecular receptor 1 for the selective sensing of anions by investigating the effect of the addition of tetrabutylammonium salts ([Bu4N]+X, X = F, Cl, Br, I, AcO, HSO4, and H2PO4). Receptor 1 (Figure 1) was particularly important as a chemosensor for fluoride owing to its noticeable color change in the presence of F ions.

be unstable at room temperature. Subsequent heating of 2 in CH3CN in the presence of I2 for 45 min afforded the desired receptor 1 in 82% yield. To look into the orientation of hydrogen bond donors around the carbazole motif, we optimized the structure by the AM1 method [45] (Figure 2). It is evident from Figure 2 that the two catechol units do not lie in the same plane as the carbazole unit.

Figure 2: The AM1 optimized structure of receptor 1 (heat of formation = 8.29 kcal/mol).

Interaction studies
Figure 1: The structure of the indolocarbazole-based chemosensor 1.

UVvis study
The anion-binding properties of receptor 1 were investigated by UVvis, fluorescence and 1H NMR spectroscopic methods. The sensing ability of chemosensor 1 with a series of tetrabutylammonium salts ([Bu4N]+X, X = F, Cl, Br, I, AcO, HSO4, and H 2 PO 4 ) in CH 3 CN/H 2 O (4:1 v/v) was monitored by UVvis absorption studies and by naked-eye observation. The tetrabutylammonium salt (TBAX) under investigation was

Results and Discussion

Synthesis
Receptor 1 was synthesized [43,44] according to Scheme 1. Condensation of indole with 3,4-dihydroxybenzaldehyde by the reported procedure yielded intermediate 2, which was found to

Scheme 1: Synthesis of receptor 1.

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Figure 3: Color changes of receptor 1 (A) (c = 1.1 104 M) in CH3CN/H2O (4:1 v/v) on addition of tetrabutylammonium salt (TBAX), X = F (B), Cl (C), Br (D), I (E), AcO (F), HSO4 (G), and H2PO4 (H) (left side); green fluorescence observed on addition of F ion to receptor 1 (right side).

added to a solution of receptor 1 (c = 1.1 104 M) in the above noted solvent mixture. In the naked-eye experiments, receptor 1 (c = 1.1 104 M) in CH3CN/H2O (4:1 v/v) showed distinct color changes from light violet to dark orange and pale pink, respectively, in the presence of two equivalent amounts of TBAF and TBAOAc (Figure 3). In the fluorescence study, the sky blue color of 1 changed to a green color on the addition of TBAF. Importantly, the receptor was found to be insensitive to the addition of large excess of Cl, Br, I, HSO4, and H2PO4 (even up to 100 equiv). The change in color was due to the deprotonation of phenolic OH groups followed by hydrogen bonding with fluoride ions. The strong hydrogen bonding to, or deprotonation/protonation of, the indolocarbazole moiety might modulate the electronic properties of chromophore [46] and give rise to significant color changes. The interaction of receptor 1 (c = 1.1 104 M) with F was investigated in aqueous CH3CN solvent in more detail by UVvis

spectroscopic titration (Figure 4). Receptor 1 itself displays two absorption bands at 283 and 338 nm in CH3CN/H2O (4:1 v/v). Upon the gradual addition of F, the absorbance increases by different extents. On increasing the concentration of F, two new absorption bands appear at 408 and 491 nm, with the effect that the solution instantaneously changes color from light violet to dark orange. These two new bands can be ascribed to the deprotonated receptor. Figure 4 shows the F-induced UVvis spectral change of receptor 1 at different concentrations of fluoride ion in CH3CN/H2O (4:1 v/v) (left side). A similar, but less remarkable spectral change, was observed upon addition of AcO (right side) where a color change from light violet to light pink was achieved upon the addition of 10 equiv of AcO. The spectral behavior indicated that deprotonation of the phenolic OH as well as NH groups by F (Scheme 2), and not hydrogen bonding to it, is responsible for the drastic color change [47], as a result of a change in the optical properties of chromogenic indolocarbazole skeleton. This is in agreement with the NMR titration data. Such deprotonation was related to

Figure 4: UV spectral change of receptor 1 (c = 1.1 104 M) upon gradual addition of [Bu4N]+F (left side) and [Bu4N]+AcO (right side) in CH3CN/ H2O (4:1 v/v) (c = 1.1 104 M).

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Scheme 2: Schematic representation (the circles represent the indolocarbazole moiety) of the two-step process leading to receptor deprotonation with basic fluoride anions.

the acidity of the H-bond donor site and the particular stability of the [HF2] ion. The stoichiometry of 1 with F was determined to be 1:2 from the Job plot [48] (as shown in Figure 5).

occurred at a slightly higher concentration of acetate than fluoride due to higher electronegativity, smaller size, and higher basicity of F ions, which make them bind strongly with receptor 1 [49]. The binding constants (K a ) of receptor 1 (Table 1) with fluoride and other ions were determined by considering a hydrogen-bonded complex with the first two equivalents of anions in a 1:2 ratio of receptor and anion complex, and that, subsequently, the second equivalent of anion (addition of excess F ion) abstracts a HF fragment to give [HF2].
Table 1: Association constantsa of receptor 1 (R1) with [Bu4N]+X salts (X = F, Cl, Br, I, AcO, HSO4, and H2PO4) in CH3CN/H2O (4:1 v/v) determined by UVvis and fluorescence methods.

Guests UVvis method F Cl Br I AcO HSO4 H2PO4

aAll

R1 (Ka M1) Fluorescence method 8.21 104 3.62 103 2.47 103 2.27 103 1.04 104 2.25 103 6.65 103

Figure 5: The Job plot of 1 with fluoride ion from UV method in CH3CN/H2O (4:1 v/v).

Parallel investigations were carried out with a series of other anions (Cl , Br , I , AcO , HSO 4 , and H 2 PO 4 ). Similar phenomena with negligible perturbations of UVvis absorption were observed with no noticeable change in color in the cases of Cl, Br, I, HSO4, and H2PO4, even at levels of up to 100 equiv. Similar anion-sensing properties were also observed in the more polar solvent DMSO (all UVvis spectra in supporting information). Deprotonation of receptor 1 also took place with the basic anion AcO and the development of a light pink color was observed after the addition of excess anion. No deprotonation took place in the presence of less basic anions such as Cl, Br, I, HSO4, and H2PO4. The receptor 1 is able to bind fluoride ion more strongly than other anions, since the catechol moiety is particularly effective in binding smaller anions. The deprotonation

3.62 104 7.92 103 4.29 103 4.13 103 1.21 104 1.14 103 1.32 103

errors are 0.8%.

A higher association constant was observed for fluoride ion than for other ions due to its strong hydrogen-bonding ability, small size, and better selectivity which resulted in a strong binding with receptor 1 [50].

Fluorescence study
Fluorescence spectroscopy studies were also carried out in order to evaluate the ability of 1 as a fluorescent anion sensor. Significant quenching of the fluorescence of 1 was observed upon addition of F ions to the solution of 1 (Figure 6, left side). In comparison, other anions, with the exception of AcO (Figure 6, right side), hardly altered the emission of 1. A large
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Figure 6: Fluorescence change of receptor 1 (c = 4.475 105 M) upon gradual addition of [Bu4N]+F (left side) and [Bu4N]+AcO (right side) in CH3CN/H2O (4:1 v/v) (c = 4.475 105 M) (max = 443 nm).

quenching of intensity with respect to other anions (Figure 7, right side) was observed at 439 nm upon the addition of 2.0 equiv of [Bu4N]+F. These results indicate that formation of hydrogen-bonded complex or deprotonation/protonation occurs by forming the anion of receptor 1; the excited state was modified considerably leading to the quenching of fluorescence. A commonly accepted mechanism for the quenching phenomenon involves an inversion between the strongly emissive * and the poorly emissive n * states of this fluorophore. Such a quenching results from a hydrogen bond interaction of phenolic OH with anions, which leads to the stabilization of the n* state with respect to the * state and a subsequent decrease in the fluorescence emission intensity [51].

Analogous investigation of fluorescence was carried out with other [Bu4N]+X salts (X = Cl, Br, I, HSO4, and H2PO4). In all cases, only slight quenching occurs on the gradual addition of the anions (Cl , Br , I , HSO 4 , and H 2 PO 4 ) to receptor 1. The spectral variations observed for receptor 1 on titrating with different anions are given in the supplementary information.
1H

NMR study

The interaction of receptor 1 with F was corroborated by 1H NMR experiments carried out in DMSO-d6 (1 has only limited solubility in CD3CN). A partial 1H NMR spectrum of receptor 1 is shown in Figure 8. It was found that the aromatic proton

Figure 7: Binding constant calculation curves for receptor 1 vs F, Cl, Br, I, AcO, HSO4, and H2PO4 (left side); fluorescence spectra of 1 after addition of 1:1 equivalent of receptor and anions (F, Cl, Br, I, AcO, HSO4, and H2PO4) (right side).

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signals underwent upfield shifts with increasing F concentration. In the presence of equivalent amounts of [Bu4N]+F, the signal for phenolic OH protons of 1 underwent large downfield shift ( = 1.34 ppm) and the proton signal was broadened. These observations further indicated that the first added F establishes an H-bond interaction with the OH subunit of 1, while an excess of F induces the deprotonation of the catechol moieties and NH proton, which brings electron density onto the -conjugated framework through bond propagation, thus causing a shielding effect and inducing upfield shift of aromatic protons. The above mentioned results indicate that receptor 1 exhibits selective sensing for F (F > AcO >> other anions) in an appropriate solvent.

complex with anions by multiple hydrogen-bonding interactions. Among the anions, only receptor 1 has higher selectivity for F and leads to a distinct color change that can be observed by the naked eye. The binding results with a series of anions suggest that receptor 1 efficiently binds F as established by UVvis, fluorescence and 1H NMR spectroscopic methods. As a colorimetric anion sensor, the indolocarbazole-based receptor 1 displayed highly selective coloration for F ion even in the presence of other anions.

Experimental
General details
All reactions were carried out under a nitrogen atmosphere. Solvents were dried before use. Solvents for spectroscopic measurements were of spectroscopic or HPLC grade. The 1H NMR spectra were recorded on a Bruker AM-500 spectrometer. The 1H NMR chemical shift values are expressed in ppm (). UVvisible and fluorescence spectra measurements were performed on a JASCO V530 and a PerkinElmer LS-55 spectrofluorimeter, respectively. Receptor 1 and guest anions were dissolved in UV-grade acetonitrile and water (4:1 v/v). The corresponding absorbance values for receptor 1 were noted during titration and used for the determination of binding constant values. Binding constants were determined by using the expression A0/A A0 = [M/(M C)](Ka1 Cg1 + 1), where M and C are molar extinction coefficients for receptor and the hydrogen-bonding complex, respectively, at selected wavelengths, A0 denotes the absorbance of the free receptors at the specific wavelength, and C g is the concentration of [Bu4N]+X (X = F, Cl, Br, I, AcO, HSO4, and H2PO4 anions). The measured absorbance A0/A A0 as a function of the inverse of the guest anion concentration fits a linear relationship, indicating a 1:2 complexation of the receptor and anions. The ratio of the intercepts to the slope was used to determine the binding constant Ka. Geometric optimization of their stable conformation of receptor 1 at the AM1 level was carried out using the minimal valence basis as STO 3G in ArgusLab 4.0.1 software suite. We have refrained from citing calculated total energy value, the calculation being for molecule only in the gas phase.

Figure 8: 1H NMR spectra of receptor 1 (bottom), 1 with [Bu4N]+F 1:2 [receptor 1:(Bu4N)+F] (middle) and excess [Bu4N]+F (top).

The fluoride- and acetate-induced deprotonation process is reversible as evidenced from the addition of CH3OH. The addition of the polar protic solvent methanol results in a gradual decrease of absorbance in UVvis studies. This is presumably because the presence of a relatively high amount of protic solvent disfavors the formation of the deprotonated receptor. However, in the wateracetonitrile system, no color changes were observed upon addition of organic bases such as triethylamine whilst the addition of excess [Bu4N]+OH can deprotonate receptor 1 and induce a color change.

Receptor 1
3,3-Bis(indolyl)-3,4-dihydroxyphenylmethane (2, 0.5 g, 1.41 mmol) in a round-bottom flask containing dry acetonitrile (5 mL), I2 (2 mol %) was added and the mixture refluxed for 45 min. The solid obtained was filtered, dried and recrystallized from a mixture of DMFCHCl3. Yield 82%, mp 258 C; 1H NMR (500 MHz, DMSO-d6): (ppm) 10.53 (s, 2H), 8.63 (bs, 4H), 7.24 (d, J = 8 Hz, 2H), 7.12 (d, J = 8 Hz, 2H), 6.94 (t, J = 7.5 Hz, 2H), 6.80 (t, J = 7.5 Hz, 2H), 6.68 (d, J = 8 Hz, 2H),
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Conclusion
In conclusion, a new colorimetric receptor 1 based on indolocarbazole was synthesized in high yield, which can form 1:2

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6.48 (d, J = 7.4 Hz, 2H), 6.43 (s, 2H); 13C NMR (125 MHz, DMSO-d6): (ppm) 145.1, 143.9, 137.1 (for two carbon), 135.1 (for two carbon), 125.9, 120.4, 119.4, 118.59, 118.0, 115.4, 115.1, 11.0, 110.1; FTIR (KBr, cm1): 3472, 3430, 1521, 1457, 1262, 1224; C30H20N2O4 (473.1496); Anal. Calcd C, 76.26; H, 4.27; N, 5.93; O, 13.54; found C, 76.35; H, 4.19; N, 5.73; O, 13.60; HRMS (MH+ + 2): 475.21.

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Supporting Information
Supporting Information File 1
13C

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NMR and mass spectra of the synthesized compound R1 and its UVvis and fluorescence spectra in the presence of different anions (Cl, Br, I, HSO4, and H2PO4). [http://www.beilstein-journals.org/bjoc/content/ supplementary/1860-5397-6-12-S1.pdf]

Acknowledgements
We thank DST [W.B. Government, project no. 694 (sanc.)/ST/ P/S&T/6G-6/2007] for financial support. GH thanks CSIR, New Delhi for a fellowship during the course of work. Also thanks are due to Prof. S. P. Goswami for providing some instrumental facilities.

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