BIOFLO III

THE MICROPROCESSOR
CONTROLLED
LABORATORY-SCALE
FERMENTOR

MANUAL NO.: M1226-0050/N

NEW BRUNSWICK SCIENTIFIC CO., INC.

BOX 4005 . 44 TALMADGE ROAD . EDISON, NJ 08818-4005 . 732-287-1200
TELEPHONE: 800-631-5417 . FAX: 732-287-4222 . TELEX: 4753012 NBSCO
INTERNET: http://www.nbsc.com/ . E-MAIL: [email protected]
WARRANTY

Every instrument manufactured by the New Brunswick Scientific Co., Inc. is

warranted to be free from defects in material and workmanship. This apparatus, with
the exception of glassware, lamps and electrodes (where supplied), is warranted for
one year against faulty components and assembly and our obligation under this
warranty is limited to repairing or replacing the instrument part thereof, which shall
within one year after date of shipment, prove to be defective after our examination.
This warranty does not extend to any NBS products which have been subjected to
misuse, neglect, accident or improper installation of application; nor shall it extend to
products which have been repaired or altered outside the NBS factory without prior
authorization from New Brunswick Scientific Co., Inc.. In addition to the above, all
biological shakers shipped to the U.S.A. and Canada carry an additional one-year
warranty.
IMPORTANT NOTICE:

RETURN MATERIAL AUTHORIZATION POLICY

IF SERVICE IS REQUIRED:

PLEASE CALL OUR SERVICE DEPARTMENT AT

1-800-237-2298

PLEASE DO NOT RETURN ANY EQUIPMENT FOR SERVICE

WITHOUT A RETURN AUTHORIZATION AND NUMBER WHICH
CAN BE OBTAINED FROM OUR SERVICE DEPARTMENT.

THE RETURN AUTHORIZATION NUMBER MUST APPEAR ON

THE OUTSIDE OF ALL CARTONS.
 TABLE OF CONTENTS

CHAPTER 1: INTRODUCTION
1.1 Scope of Manual
1.2 Description of Equipment
1.3 Description of Vessel
1.4 Agitation System
1.5 Temperature Control
1.6 Aeration
1.7 pH Control
1.8 DO Control
1.9 Foam Control
1.10 Medium Flow Control
1.11 Exhaust System
1.12 Sampling System
1.13 Flowmeter

CHAPTER 2: INSTALLATION
2.1 Inspection
2.2 Description of the BioFlo III Console
2.3 Installation of Console
2.4 Service Connections
2.5 Recorder Connections

CHAPTER 3: PREPARATION AND OPERATION

3.1 Cleaning of Vessel
3.2 Vessel Assembly
3.3 Preparation Before Sterilization
3.4 Sterilization Procedure
3.5 pH Probe Preparation
3.6 pH Probe Calibration
3.7 Dissolved Oxygen Probe Calibration
3.8 Selection of Medium Flow Rate
3.9 Preparation for Operation
3.10 "D.O. Active" System
3.11 Sampling Procedure
3.12 Shut-Down Procedure

CHAPTER 4: MAINTENANCE
4.1 General
4.2 Console Cleaning
4.3 Periodic Inspection
4.4 Vessel and Tubing Cleaning
4.5 Probe Maintenance and Storage
4.6 The Agitator Bearing Housing
4.7 Replacement Part List
 4.8 Electrostatic Device (ESD) Warning
4.9 ESD Precautions

APPENDICES
I BioFlo III Operational Tips
II Communication Protocols for NBS RS-232/422

DRAWING LIST
 CHAPTER 1
INTRODUCTION

1.1 SCOPE OF MANUAL

This manual contains a description of the vessel, installation, operating and

maintenance instruction for the BioFlo III System 1.25, 2.5 and 5L Models
manufactured by the New Brunswick Scientific Co., Inc., 44 Talmadge Road,
P.O. Box 4005, Edison, New Jersey 08818-4005, USA.

1.2 DESCRIPTION OF EQUIPMENT

BioFlo III is a versatile bioreactor that provides a fully equipped state of the art
fermentation system in one compact package. BioFlo III can be employed for batch
or continuous culture with microprocessor control of pH, DO, agitation, temperature,
nutrient feed, and electronic foam control.

1.3 DESCRIPTION OF VESSEL

The vessel parts consist of a stainless steel head plate, a flanged glass tube (thick
walled) vessel body which is detachable from the bottom dished head. The dished
head is jacketed for circulation of temperature controlled water. Four sterilizable
polypropylene compression ports are provided in the glass wall for the addition of
antifoam and nutrients, as well as for vessel overflow in continuous culture studies.
Ports are provided in the headplate for: Inoculation, base and acid addition, a
thermowell for Resistance Temperature Detector, a Sparger, a Harvest Tube, a
Sampler, an Exhaust Condenser, Dissolved Oxygen and pH Electrodes. The drive
bearing housing is also located on the headplate.

1.4 AGITATION SYSTEM

A removable agitation motor located on top of the bearing housing is connected to the
agitation shaft with a multi-jaw coupling. It can be easily disconnected while
autoclaving the vessel and replaced after sterilization. The motor will provide
agitation
speed range of 50 to 1000 ± (2 RPM + 0.5% setting).

1.5 TEMPERATURE CONTROL

The culture temperature may be selected in the range from 4°C to 80°C (± 0.1°C) and
is controlled by a microprocessor based PI (Proportional and Integral) controller. The
media temperature is sensed by an RTD (Resistance Temperature Detector)
submerged
in the thermowell.

M1226-0050 1
M1226-0050 2
1.6 AERATION

Sterile air is introduced into the medium through the ring sparger, and is controlled by
the needle valve of the flowmeter. It is able to provide 1.5 (working volume) of sterile
air thru 0.2 μm replaceable cartridge filter (Fig. 1). The filter is sterilizable with the
vessel. With the system, Oxygen Transfer Rates of 350 mMO2/L/Hr may be obtained.

1.7 pH CONTROL

pH is controlled in the range of 2.00-12.00 (± 0.01). The pH is sensed by a glass

electrode. Control is maintained by PID controller which operates two peristaltic
pumps, connected to acid and base addition ports (Fig. 1).

1.8 DO CONTROL

DO is controlled in the range of 5-95% (± 1%). It is sensed by the DO electrode and

control is maintained by the PID controller which changes the speed of agitation.

1.9 FOAM CONTROL

Foam is controlled during batch fermentation by the antifoam probe which is located
in the headplate. The controller operates the antifoam addition pump that adds
chemical defoamer through the port in the wall of the vessel (Fig. 1). Sensitivity and
time adjustments are made on potentiometers located on a small printed circuit board
mounted on the underside of the electronic shelf, easily accessible when the rear door
is open. The potentiometer on the right (P1) is for sensitivity adjustment and the
potentiometer on the left (P2) is for percent pump on time adjustment.

1.10 MEDIUM FLOW CONTROL

A nutrient feed peristaltic pump is provided for use during continuous culture
fermentation. The maximum flow rate can be found in section 3.8.

1.11 EXHAUST SYSTEM

The exhaust gases pass into the exhaust condenser where moisture is removed and
returned to the vessel. The air remaining passes through the 0.2 μm exhaust filter and
goes to the atmosphere (Fig. 1).

1.12 SAMPLING SYSTEM

The sampler is attached to a sampling tube that extends to the liquid of the vessel.
The
sampler has a rubber suction bulb to facilitate collection of representative samples

M1226-0050 3
 without contamination. A 25mL screw-cap container serves as a reservoir (Fig. 2).
1.13 FLOWMETER

The flowmeter is a simple, precise means of indicating flow rates in fluid systems.
Their design is based on a variable area principle. BioFlo III is equipped with a 0.2
to 4.0 SLPM flowmeter.

NOTE: 5L Unit uses an interchangeable tube to allow increased flow

to 7.5 SLPM. Tube is replaced by removing plug on top of
flowmeter (Fig. 3).

M1226-0050 4
 CHAPTER 2
INSTALLATION

2.1 INSPECTION

Unpack the BioFlo III and carefully inspect for any apparent damage which may have
occurred during transit. Report any obvious damage to the carrier and to New
Brunswick Scientific Co., Inc..

Verify that the cabinet, its accessory kit (M1151-0010) and manual are correct.
Inspect the vessel assembly, probe kit and vessel accessory kit (M1226-0014).

For initial set up refer to Chapter 3.

2.2 DESCRIPTION OF THE BIOFLO III CONSOLE

1. Front Panel (Fig. 4)

Right hand side part

• Agitation Switch - to start or stop the agitation motor.
• Increase/Descrease Switch - to raise or lower the set point of the
parameter selected. Also used during calibration of pH and DO.
• Selector Switch - to select pH, DO, agitation, temperature or
nutrient readings to be displayed.
• Mode Switch - to select control, setpoint, span or zero mode.
• DO Control Active Switch - push in this switch to "ON" position.
DO will cascade with agitation.

Left hand side part

• DO electrode receptacle.
• pH electrode receptacle.
• RTD (Resistance Temperature Detector) receptacle.
• Antifoam (on level) and ground lead sockets.
• Agitation motor connector.
• Flowmeter, Flowrate adjustable valve and barbed connector for air.

2. Rear of Cabinet (Fig. 5)

• Input water line barbed connector.

• Drain line barbed connector.
• Air inlet line barbed connector.
• Line cord.
• Fuses
3. Left Hand Side of the Console

M1226-0050 5
 • 9 pin D-connector for recorder output.
• 25 pin D-connector RS232/422.

4. Right Hand Side of the Console

• Switch panel (Fig. 6).

The switch panel located on the right-hand side of the console includes: the
Main Power Switch which controls the power to the system; the Prime Switch
which fills the recirculation system with water before normal operation, and
after each autoclaving. Antifoam, nutrient, base and acid switches operate the
addition pumps. The top position "ON", is for continuous pumping to prime
the tubing. The bottom switch position "AUTO" is for automatic pump
operation. The center position is "OFF".

• External nutrient pump control (Fig. 7).

The 6 lug terminal strip allows for external control of the nutrient pump mounted on
the front panel.

Requirements: A controlled DC voltage source to supply a voltage of +5 VDC to

15 VDC and have a minimum current rating of 20mA.

Connections: a. Remove the three jumpers from lugs 1 and 2, 3 and 4, 5

and 6 of the terminal strip.
b. Connect the positive control source of lug 3 of terminal
strip.
c. Connect the ground lead from control source to Lug 1
of terminal strip.

NOTE: The nutrient pump switch adjacent to the nutrient pump

will not function with these jumpers removed.

M1226-0050 6
2.3 INSTALLATION OF CONSOLE

1. Position the BioFlo III console on a firm and level surface in an area where
services are readily available.

2. Level the horizontal surface of the base with four leveling glides if necessary.

3. Set the power and agitation switches to their off position.

4. Check the specification plate on the rear of the unit and plug the line cord into
a suitable electrical outlet.

2.4 SERVICE CONNECTIONS

Water: 20 max. PSIG, 50 μm filtration, trim valve adj. 1 1/2 turns open.
Air: 10 PSIG max.

2.5 RECORDER CONNECTIONS

Recorder Output Option: Recorder Cable

0.0 - 1.0 Volt DC M1132-3022

PINS
1, 2 TEMP
3, 4 pH
5, 6 D.O.
7, 8 AGITATION

Grounds are pins 2, 4, 6 and 8 pins.

M1226-0050 7
 CHAPTER 3
PREPARATION AND OPERATION

3.1 CLEANING OF VESSEL

1. Fill the vessel with a mild detergent and water solution. Let stand for one hour
then scour thoroughly. Use a brush on both inside and outside surfaces.

2. Drain the vessel and rinse several times with tap water. Repeat rinsing with
distilled water and let dry.

3.2 VESSEL ASSEMBLY

1. Slide impellers on the shaft of the bearing housing and clamp them down.
Lower impeller should be positioned about 1/4 inch above the bottom of the
baffle. Upper impeller one to one and half impeller diameters above lower
impeller (Fig. 8).

Working From the Underside of the Headplate (Fig. 9)

2. Insert the sparger tube in the sparger port (Fig. 10).

3. Insert the harvest tube in the harvest port (Fig. 11).

Working From the Top of the Headplate

4. Insert the thermowell tube into the temperature port* (Fig. 12).

5. Insert the sampler assembly into the sample port (Fig. 2).

6. Install foam probe in the headplate (Fig. 13).

7. Install the baffle assembly inside of the glass jar by pressing the two edged
baffle together (Fig. 14).

8. Install four polypropylene ports or plugs in the wall of the glass jar. Be sure to
use a blind plug when a port is not in use (Fig. 15).

9. Lubricate the headplate O-ring with silicone grease.

10. Place the headplate on the flange of the vessel and lock it to the clamping ring
with knurled screws.**

11. Install pH and DO probes (Fig. 16, 17, 18).*** Coat probe with glycerin

M1226-0050 8
 before inserting into the port in the headplate.****

12. Insert exhaust condenser into the exhaust condenser port. Connect the
exhaust filter on the top of the condenser with flexible tubing (Fig. 20).

13. Slide 2" long, 1/4" inside diameter silicone tubing on the top of the sparger
tube, then connect air filter to it (Fig. 10).

NOTES:

* Add a few drops of glycerin into the thermowell tube before inserting
RTD.

** Do not overtighten the clamping ring to the headplate. Same to the

clamping screws for the glass tube reactor and the vessel base
(vessel heater).

*** When the inoculation port is used for installation of Phoenix DO

probe, the DO port is converted to the inoculation port (Fig. 19).

**** Membrane of the probe must be free of glycerin.

3.3 PREPARATION BEFORE STERILIZATION

Before sterilization, perform the following procedure:

1. Fill vessel with water to its working volume.

2. Place the vessel assembly on the three pins located in the base of the cabinet.

3. Position the motor assembly on the top of the bearing housing. Connect the
motor cable to the receptacle on the face of the cabinet.

4. Connect cables from all probes (pH, DO and antifoam) to their respective
sockets on the front face of the cabinet.
Ground lead from the antifoam socket is to be connected to the ground pin
on the headplate.

5. Insert RTD in the thermowell on the headplate and connect it to the socket
on the cabinet.

6. Connect the inlet filter on the top of the sparger tube to the hose barb in the
face of the cabinet with flexible tubing.

7. Connect water line to rear of the cabinet. Adjust water pressure to 20 PSIG
max.

M1226-0050 9
 8. Connect drain line to the rear of the cabinet.

9. Connect air line to the rear of the cabinet. Adjust air pressure to 10 PSIG
max.

10. Connect the quick connects of plastic water lines to the vessel base and the
exhaust condenser.

11. As soon as the power switch is turned ON, press the prime switch ON and
hold for approximately 10 seconds or until water runs out the drain line at
the rear of the unit.

IMPORTANT NOTE:
When turning the power switch ON, particularly at initial installation.
It is important that the unit is properly primed by filling the water
circulation loop to prevent the circulating pump from running dry
for any period of time.

12. Check that the machine functions properly.

13. Pull out the pH probe from the pH port and calibrate it (see Section 3.6).

3.4 STERILIZATION PROCEDURE

1. Remove the motor from the top of the vessel and place it on the motor mount
at the top of the cabinet.

2. The housing cover is to be positioned on the top of the bearing housing before
sterilization (Fig. 26).

3. Disconnect air line of the inlet filter side.

4. Disconnect all probes and remove probe cables.

5. Disconnect vessel heater and exhaust condenser water lines.*

6. Remove the sampler rubber bulb then insert glass wool into sampler port and
close sampler valve.

7. Empty and clean the vessel.

8. Fill vessel with medium or sterilized water.**

9. Sterilize the complete assembly consisting of jar, headplate and components
of headplate by inserting in an autoclave.

M1226-0050 10
 10. Autoclave at a temperature of 121°C at 15 PSIG for 25 minutes.

NOTES:
* When disconnecting the water lines, always disconnect the Water-In
line first.

CAUTION:
NEVER DISCONNECT THE QUICK CONNECT WATER LINES
WITH VESSEL BASE AND EXHAUST CONDENSER WHEN THE
POWER IS “ON” AND THE CURRENT READING OF THE VESSEL
TEMPERATURE IS HIGHER THAN THE SET POINT.

** For continuous culture, vessel should not be sterilized empty. Use at

least 100mL of sterilized water. Probe tips must be moist during
sterilization. After autoclaving medium will be brought from the
medium reservoir, sterilized separately. For batch fermentation the
medium is sterilized with the vessel.

CAUTION:
WHEN AUTOCLAVING THE GLASS VESSEL, IT
MUST BE VENTED AT ALL TIMES. DURING
STERILIZATION OF THE GLASS REACTOR, VESSEL,
CONTENTS, AND ELECTRODES IT IS IMPORTANT
THAT THE PRESSURE BUILT UP IN THE
AUTOCLAVE IS RELEASED ONLY WHEN
TEMPERATURE HAS DROPPED BELOW 90°C. SLOW
EXHAUST IS REQUIRED.

If after autoclaving, most of the liquid has exhausted from the vessel, the autoclave is
exhausting too quickly. Adjust the autoclave to exhaust more slowly. Attach a piece
of tubing with some non-absorbent material such as glass wool or non-absorbent
cotton and some foil wrapped on the ends to one of the addition ports. This helps
the vessel to vent more easily during autoclaving. Immediately crimp the foil and
close off the vent tubing to maintain sterility.

3.5 pH PROBE PREPARATION

Inspect probe for possible shipping damage. If damage is observed notify the Service
Department of the New Brunswick Scientific Co., immediately.

M1226-0050 11
 Check the level of the reference electrolyte. It should be about 1cm below the filling
orifice, which is closed with a rubber T stopper. To add reference electrolyte, take the
filling pipette (P0740-4820) and fill it with Viscolyte B (P0860-0130) Electrolyte.

NOTE: The two chambers are filled with same Reference Electrolyte.
The total volume of Reference Electrolyte held be the electrode
is approximately 30mL. During normal operation the two rubber
stoppers are to be removed.

3.6 pH ELECTRODE PROBE CALIBRATION

pH probe is to be calibrated prior to autoclaving vessel.

Connect electrode to probe cable.

Turn the agitation switch OFF.

Turn ON power switch.

NOTE: The pH measuring system is calibrated using two buffer

solutions of a known pH.

1. Set the selector switch to pH.

Set the mode switch to "ZERO".

Immerse the pH probe into an external pH 7.00 buffer solution.

Set the display to read the pH value of the buffer with INC/DEC switch.

2. Immerse the pH electrode in a second external buffer solution which is several

pH units above or below the pH selected in the previous step.

Set the mode switch to "SPAN".

Set the display to read the value of the second buffer solution with the INC/
DEC switch.

NOTE: After autoclaving it is recommended that the calibration is

checked by taking a sample from the vessel and measuring
the pH with an external pH meter.

M1226-0050 12
3.7 DISSOLVED OXYGEN PROBE CALIBRATION

Probe is to be calibrated after autoclaving vessel.

1. There are two methods of obtaining zero on DO.

METHOD 1 (Less Accurate)

Remove the DO probe cable from the DO probe. Set the selector switch to
"DO".

Set the mode switch to "ZERO". With the INC/DEC switch adjust the display
reading on the DO to read zero. Connect the DO probe cable to the probe.

METHOD 2
Connect the DO probe cable to the DO probe. Set the selector to DO.

Set the agitation speed to 500 RPM.

Sparge Nitrogen into the vessel until the display is stable for approximately
5 minutes.

Set the mode switch to the "ZERO" position.

With the INC/DEC switch adjust the display for zero reading.

2. Set the "SPAN".

Set the selector to DO.

Set the mode switch to "SPAN". Vigorously sparge air into the vessel. Set the
agitation speed to 500 RPM.

After about 30 minutes observe the reading on the display. After the DO
reading has stabilized. With the INC/DEC switch adjust the DO display
reading
to 100.

3.8 SELECTION OF MEDIUM FLOW RATE

Medium flow rate depends on the bore size of the tubing and percentage of the time
that nutrient pump will be running (at constant speed).

Nutrient Pump (Seconds):

M1226-0050 13
 Set Point Pump ON (Sec.) Pump OFF (Sec.)
100.0 10 3
90.0 9 4
80.0 8 5
70.0 7 6
60.0 6 7
50.0 5 8
40.0 4 9
30.0 3 10
20.0 2 11
10.0 1 12
00.0 0 00

Pump flow rates at 100% set point.

Flow rates (mL/min) Silicone tubing only

Tubing internal diameter
Freq. RPM 0.5mm 0.8mm 1.6mm 3.2mm 4.8mm
1/50" 1/32" 1/16" 1/8" 3/16"

50 Hz 5 .083 .19 .84 3.05 6.10

60 Hz 6 0.10 .23 1.00 3.65 7.32

3.9 PREPARATION FOR OPERATION

1. Position the vessel on BioFlo III console. Connect the vessel base and the
exhaust condenser with water lines.*

2. Carefully place the DC motor on the vessel assembly.

3. Add glycerin to the thermowell and insert (RTD) temperature probe.

4. Turn power to "ON" position and "PRIME" the system.

5. Connect all probe cables to their respective receptacles.

6. Calibrate DO probe (see sec. 3.7).

7. Set agitation to desired speed and then depress agitation selector to the "ON"
position.

8. With "INC/DEC" switch adjust the displayed value of temperature, pH, DO

and Nutrient to the desired set point.

9. Set the "DO ACTIVE" switch to "DO ON" or "DO OFF" position.
10. Adjust the airflow rate on the flow meter to the desired flow rate.

M1226-0050 14
 NOTE: Aeration is required whenever the agitation setpoint is greater than
750
rpm. NBS suggests a minimum airflow rate of 0.25 vvm when running
at speeds of 750 rpm or more.

*NOTE: Always connect the water out line first.

3.10 D.O. ACTIVE SYSTEM

The system is designed to control D.O. within a range of 5-95%. D.O. regulation is
accomplished by PID control of agitation speed between 50 and 1000 rpm. When the
D.O. actual value is above the D.O. set point, the agitation rpm will decrease until the
D.O. set point is reached.

As the D.O. level drops below the set point value, agitation will continue to increase
until the D.O. set point is reached. Any further increases in agitation, if needed, must
be introduced manually.

To set up and use D.O. Mode proceed as follows:

1. Set selector to Agitation.

2. Set mode to set point.
3. Adjust rpm with Inc/Dec to the minimum rpm value you wish for D.O. control.
4. Press D.O. control active and observe LED is now on for both D.O. control
active and D.O. control max rpm.
5. Adjust rpm with Inc/Dec to maximum value you wish for D.O. control.
6. Set mode switch to control.
7. D.O. mode is now controlled by regulation of the Agitation (rpm) between the
limits that have been set (minimum/maximum set points).

3.11 SAMPLING PROCEDURE (Fig. 2)

To sample the culture, proceed as follows:

1. Check that the value on the sampler is closed.

2. Loosen the sample bottle so that it is not sealed against the sampler gasket.

3. Squeeze the rubber bulb and then tighten the bottle to seal against the sampler
port.

4. Open sampler value and slowly release the bulb.

5. When desired volume of sample is obtained, close the valve.

M1226-0050 15
 6. Remove bottle with sample from the sampler. Place the cap from a new bottle
onto the bottle containing sample and install the new bottle in the sampler and
make sure that the sample bottle is firmly sealed against the sampler gasket.
Use aseptic techniques.

7. Repeat step 1 through 6 until desired number of samples are taken.

3.12 SHUT-DOWN PROCEDURES

To shut-down the system proceed as follows:

1. Rotate flowmeter dial to zero.

2. Set the Agitation and Power switches to OFF.

3. If the system is not to be used for several days, disconnect power plug.
Remove and clean vessel, and associated components.

NOTE: DO NOT WASH THE FILTERS OR GET THEM WET.

M1226-0050 16
 CHAPTER 4
MAINTENANCE

4.1 GENERAL

Preventive maintenance is performed to keep equipment in proper working condition.

When periodically performed, it will result in longer life for the equipment and reduce
time lost due to equipment failure.

4.2 CONSOLE CLEANING

At least once a month, clean all metal parts of unit. Use a damp cloth moistened with
water or mild detergent. If a detergent is used, remove all excess by clean water
washing.

4.3 PERIODIC INSPECTION

At three month intervals perform the following checks and inspections with all
switches
"OFF" and incoming power disconnected.

1. Check the fuse(s) for clean contact.

2. Check all controls and accessible items (switches, knobs, fuse holders, screws,
nuts and bolts) to make sure they are properly tightened. Tighten any item
which is loose.

3. Check that all controls are free of dust and operate easily.

4.4 VESSEL AND TUBING CLEANING

After each run clean the vessel, headplate and associated parts.

4.5 PROBE MAINTENANCE AND STORAGE

Carefully read the instruction manual of probes before use.

4.6 THE AGITATOR BEARING HOUSING (Fig. 25)

Every 3-6 months, the ball bearings and the shaft seals in the bearing housing should
be
checked and cleaned. Replace the worn out bearings and shaft seals.

M1226-0050 17
4.7 REPLACEMENT PART LIST
Vessel (1.25L) Assy. M1226-2000
Vessel (2.5L) Assy. M1226-2003
Vessel (5L) Assy. M1226-2005
Inlet Filter P0200-0491 (37mm disc)
Exhaust Filter P0200-0490 (50mm disc)
RTD Assy. M1169-8002
pH Probe (1.25L, 2.5L) Liquid P0720-5325
pH Probe (1.25L, 2.5L) Gel, Ingold P0720-5582
pH Probe (1.25L) Gel, Broadley James P0720-5731
pH Probe (2.5L) Gel, Broadley James P0720-5744
pH Probe (5L) Liquid P0720-5021
pH Probe (5L) Gel, Ingold P0720-5580
pH Probe (5L) Gel, Broadley James P0720-5732
pH Cable, Liquid, Gel, Ingold P0720-2095
pH Cable, Gel, Broadley James P0720-2270
pH Cap, Liquid, Gel, Ingold P0720-5317
pH Cap, Gel, Broadley James P0720-5737
pH Probe, Electrolyte, Liquid P0860-0130
DO Probe (1.25L) (Phoenix) P0720-5440
DO Probe (2.5L) (Phoenix) P0720-5450
DO Probe (5L) (Phoenix) P0720-5460
DO Probe Cable P0720-2202
* DO Probe (1.25L) (Ingold) P0720-5565
* DO Probe (2.5L) (Ingold) P0720-5561
* DO Probe (5L) (Ingold) P0720-5562
DO Probe Cap (Ingold) P0720-5567
DO Service Kit (Ingold) P0720-5569
DO Adapter (Ingold) (1.25L, 2.5L) M1016-0900
DO Adapter (Ingold) (5L) M1226-9446
Seal Washer for Ingold DO Probe M1016-0890
Teflon Washer for Ingold DO Probe P0100-9780
O-Ring for Ingold DO Probe P0280-9163
Antifoam Probe F5-137
Hose Connect Plug M1151-9418
Hose Connect Open M1151-9430
Seal, Vessel Side Port M1151-9419
Nut, Vessel Side Port M1151-9420
Fuse, 12 Amp (100V, 120V) P0380-3122
Fuse, 2.5 Amp (100V, 120V) EF-141
Glass Vessel 1 1/4L M1151-9904
Glass Vessel 2 1/2L M1152-9903
Glass Vessel 5L M1155-9904
Fuse, 6 1/4 Amp, 220V EF-118
Fuse, 2 Amp, 220V P0380-3260

M1226-0050 18
 Bearing Housing Cover M1151-9444
Bearing Housing Cover O-Ring P0280-6093
Motor Assembly (1.25L, 2.5L) M1226-0700
Motor Assembly (5L) M1226-0800
Units shipped before April 1, 1996 (Mfg. No. M1226-3004, -3005, -3006)
Heater, 400W, 100/120V Units P0620-1330
S.S.R. (240D10), 10 Amp, 100-240 VAC Unit P0400-3011
Heater, 400W, 220/240V Units P0620-1331
Units shipped after April 1, 1996
Heater, 750W, 100/120 VAC Units P0620-1320
S.S.R. (240D45), 45 Amp, 100-240 VAC Units P0400-3151
S.S.R. (240D10), 10 Amp, 100-240 VAC Units P0400-3011
Heater, 750W, 220/240V Units P0620-1321
*NOTE: Ingold DO Probes must be used with DO Adapters.
O-RING
Description 1 1/4L 2 1/2L 5L
Head Plate P0280-8002 P0280-8062 P0280-8132
Bumper, Flat Gasket M1151-9905 M1152-9904 M1155-9902
Knob P0280-5322 Same Same
DO Port P0280-5932 Same Same
DO Port (Ingold) Washer M1016-0890 Same Same
Teflon Washer (Ingold DO) P0100-9780 Same Same
O-Ring (Ingold DO Probe) P0280-9163 Same Same
pH P0280-5912 Same Same
3/16" Port P0280-5302 Same Same
1/4" Port P0280-5322 Same Same
3/8" Port P0280-5342 Same Same
1/2" Port P0280-5912 Same Same

4.8 WARNING:
Do not attempt to change boards or electronic components unless you are a
qualified service technician. Integrated circuits are extremely susceptible
to damage from electrostatic discharge. Read and follow the precautions
in Section 4.9 before you begin.

4.9 ESD PRECAUTIONS

1. Do not remove components from their antistatic packaging until you are ready
to insert them into their sockets or install the board.
2. Before handling components or boards, touch an unpainted portion of the
system unit chassis for a few seconds.
3. Wear a wrist grounding strap, available from most electronic component
stores.

M1226-0050 19
 APPENDIX I

BIOFLO III OPERATION TIPS

GLASS VESSEL ASSEMBLY

Recommendations for prevention of cracking glass during assembly and autoclaving:

• Cracking of glass due to overtightening of assembly screws will occur

during tightening, not during autoclaving. Therefore,

1. Prior to autoclaving, tighten screws "finger tight". If a wrench is

applied at this point, turn each nut 1/2 revolution not to exceed
1 revolution.
2. Place unit in autoclaving making certain that the exhaust filter(s) is
not wet or clogged. Also, loosen the inoculation diaphragm cap
to achieve further ventilation.
3. After autoclaving, tighten inoculation port cap. Further tightening
of nuts can be done at this time with air flowing through vessel.

EXHAUST CONDENSER

1. DESCRIPTION
• There are two quick connects on the condenser body, the lower one
has valve, the upper one is without valve.
• To connect the water lines, connect the upper quick connect first, then
the lower one.
To disconnect the water lines, disconnect the lower one first, then the
upper one.
• A restriction (M1151-0140) has been installed in the water line as a
water saving device (Fig. 21). If maximum water flow to the condenser
is desired, the restriction may be removed.

2. CLEANING
• The inner assembly of the condenser can be removed for cleaning
(Fig. 22).
• To clean, pass warm water and detergent through the top of the
condenser twice (not through quick connects).
• Run clean water through once.
• Blow out with air.
• Clean exhaust condenser after each fermentation. This is most critical
when operating as a chemostat for protracted fermentation time.

M1226-0050 20
 INSTALL A DOUBLE FILTER SYSTEM
Double exhaust and double inlet filters are recommended.

• Attach a "Y" fitting to the top of the condenser with a piece of tubing. Attach
an exhaust filter on each branch. This allows you the flexibility to exchange
sterilized filters during a run should one filter become clogged, (pinch off
unused line with a clamp).

NOTE: Fifty generations of recombinant E. coli have been propagated

by BioFlo III in continuous culture. Prolonged operation as a
chemostat requires double filters.

OPERATION AS A CHEMOSTAT, SUGGESTIONS

Switch the relative positions of the antifoam and acid pumps on the console so that the
antifoam pump is now on the bottom and the acid pump is on the top.

• "Switching" pumps is achieved by switching electrical connections in the back

of the unit.
• Simply exchange the 4 plugs which go to one pump board with the 4 plugs
from the other pump board.
• It is convenient to use the newly positioned antifoam pump as the harvest
pump, (gravity is now in your favor; a continuously filled harvest tube will
prevent "grow back").
• Place L-shaped harvest tube through a side port, 3/4 of the way down to
the bottom of the vessel.

Use the antifoam probe as a level probe to actuate the harvest pump.

OTR CALCULATIONS

OTR can be estimated by titrating fixed amount of sodium sulfite, Na2SO3, with
air,

• 2SO3 + O2 CU+2 2SO4

Calibrate D.O. electrode.

• Set zero on DO

Fully oxygenate fermentor with agitation and airflow.

• Set span to 100%

M1226-0050 21
 Introduce a known amount of Na2SO3 into fermentor when fully oxygenated.

• OTR = 30,000 n mmoles/L/hr

V ΔT

n = number of moles of sodium sulfite

V = vessel volume, liter
ΔT = time taken from DO curve at two points of 50% DO min.

Actual OTR Data:

n = .7 moles (88.27 gms)
V = 5 liters (filled to center line of addition ports)
ΔT = 10 minutes
OTR = 420 mmoles/1/hr

Impeller Scheme: Rushtons, 1 diameter off bottom, 1 1/2 diameter separation

Viscosity: 1 cp
RPM: 998
air flow: 7.5 SLPM, 1 1/2 vvp (volume air per volume liquid)
catalyst: .014 moles CuSO4 • 5H2O (3.5 gms)

FACTORS AFFECTING CONTROL

The BioFlo III, BioFlo IV, BioFlo 3000, CelliGen Plus, MP40 and 80 controls most
functions using a PI (Proportional & Integral), control algorithm. The proportional
term of this algorithm is straight forward and provides a larger output for a greater
error (set point minus current value). The integral term however, is not as straight-
forward. This term integrates the error as a function of time to provide a larger output
if an error exists for a time period. Each of these terms have constants which are used
in each term of the equation. These constants have been picked based on New
Brunswick Scientific's experience with this product and several cell lines. The
integral constant is very much dependent on the response time of the system. This
response time is affected by many variables. These include medium viscosity, sensor
response time, control element response time and cell response time. These factors
affect each control loop but are especially important in the control of pH. In general,
this flow rate and acid/base concentrations for particular application must be
determined empirically by the investigator.

The first factor to consider is the pH probe. pH probes as they age and are affected by
many factors, e.g., protein accumulation, diaphragm clogging, and electrolyte
contamination. All of these things lengthen the response time of the probe. This
builds lag time into the control loop and causes the controller not to work properly.
The probe should be cleaned and reactivated on a regular basis.

The next factor is strength or concentration of the acid or base (caustic) being used.
Obviously, the higher the concentration the faster the system will respond to a given

M1226-0050 22
 addition. If the system is overshooting the set point in either direction the system
could be made to control better by diluting the appropriate reagent (acid if
undershooting and base if overshooting).

The last factor that can be controlled is the amount added over a given period of time.
Since in this system the pump is a fixed speed pump and the "ON" time is controlled
over a fixed pulse width if the flow rate is too small or too large, the controller cannot
adjust properly. Therefore, the flow rate should be adjusted by changing the bore size
of the tubing used for the peristaltic pump addition. Usually the tubing is too large
and should be adjusted downward to effect better control if unit is not controlling
properly.

Under proper conditions, pH may be controlled to within ± .02 units. However, if the
flow rate or acid/base concentration are too high, the control shown in Figure 23A
may be observed. Here, control at pH 6.00 (from 7.18) after 24-48 hours of
Saccharomyces cerevisiae fermentation was attempted using 2N HCL and 2N NaOH
with a flow rate of approximately 7.3 mL/min (9.5 mL/min for maximum flow rate).
After an initial period of accurate control, it became apparent that the ratio of flow rate
(or concentration) to bioreactor volume was too high. In this case, control to ± 0.12
unit was obtained. If the flow rate is increased still further to 13.5 mL/min by
increasing the internal diameter (I.D.) of the tubing, then control to ± 0.19 units
results. This is shown in Figure 23B. On the other hand, if the flow rate is decreased
to 4.1 mL/min by a reduction in tubing I.D., then accuracy is increased to ± 0.02 units,
after a slightly longer initial lag time (Fig. 23C).

The effects of agitation rate (or inversely, viscosity) may also be seen in Figure 23D,
where the pH control of a 1L fermentation of the same organism is attempted. The
difference in control accuracy at different agitation rates is evident (flow rate = 1.9
mL/min). Although a less optional deviation from the set point value, as shown in the
examples above, may be more than acceptable for a particular application, it is
generally desirable to eliminate such deviations in order to minimize the excessive use
of acid and base. Furthermore, the addition of excess Na+ and CL- ions (as NaOH
and HCL), along with increase in medium volume, are factors which one might also
wish to consider.

The dissolved oxygen control loop may have similar control difficulties if the probe or
electrode is not maintained on a regular basis.

M1226-0050 23
 The temperature control loop might not control well in very vicious applications
because the heat transfer rate was decreased so much the controller loses its ability to
adjust the heating and cooling times properly. Most times this can be cured or
improved by increasing the agitation and/or airflow rates and thus improving the heat
transfer. Improper temperature control might also happen after a long period of use
because there is a buildup of mineral deposits inside the cooling system. This can be
cured by flushing the system with a de-liming solution.

M1226-0050 24
 APPENDIX II
COMMUNICATION PROTOCOLS FOR NBS RS232/422

COMMUNICATIONS
The fermentor system has serial communications signals compatible with both the
RS232C and RS422 standards which allow a host computer to be interfaced directly
to the fermentor system. These signals are accessed via the 25 pin "D" connector
(serial port). Table 4 provides detailed information on pin assignments for the serial
port connector and associated signal definitions.

COMMUNICATION SET-UP - (See Table 1 and 2)

All the switch settings are read only once at fermentor power-up time. So any change
of the switch settings will not take effect until next power-up.

MODE
Non-Multi-Drop Mode - The fermentor responds to inquiry and modify set points
commands.

Multi-Drop Mode - The fermentor responds to inquiry and modify set points
commands which are prefixed with a unit number. When using the RS422 multi-drop
format, the mating connector for each fermentor unit on the RS422 link should be
fitted with a 1000 ohm, 1/2w terminating resistor. It should be connected from
IRXD+
(pin 12) to IRXD- (pin 24). The ITXD lines should be terminated similarly at the
host.
Consult the host computer manual for details and appropriate pin numbers.

HANDSHAKE - None

BAUD RATE
Baud rates are selectable and include standard rates 1200, 2400, 4800, 9600.

WORD LENGTH
In the non-multi-drop mode, 7-bit or 8-bit word length may be used.

In the multi-drop mode, 8-bit word length is required.

PARITY
Parity may be selected as none or even.

STOP BITS
2 stop bits.

M1226-0050 25
 UNIT NUMBER
The multi-drop mode requires a unique unit number assignment for each fermentor in
the multi-drop chain. All inquiries and directives and their responses in the multi-drop
mode are prefixed with the unit number. The unit number is formatted as a single
hexadecimal byte where the low-order nibble (bits 3-0) is set to a number between 0
and 15 and the high-order nibble (bits 7-4) is set to 8. Table 5 shows the valid
hexadecimal values for fermentor unit numbers. The format of a multi-drop operating
setpoint inquiry from unit 1 in hexadecimal notation is

81 52 44 0D

where 81 is the unit number

52 is the code for R
44 is the code for D
and 0D is the code for carriage return.

The response of unit 1 to this inquiry is shown in Figure 1.0d. A directive to change
the setpoints of unit 1 is shown in Figure 1.0h.

EOL CHARACTERS
The EOL (end of line character) is CR (0DH).

SERIAL PORT PROTOCOL

Two types of commands are accepted by the fermentor: Inquiry or Directive.

INQUIRIES
An inquiry command causes a requested report to be transmitted by the fermentor. All
inquiry request strings are of the form:

52H xxH 0DH

R X Carriage Return

Where R and X are standard ASCII and X is as shown in Table 3.

Table 3 - Inquiry Responses

X xx Reponse Response Header

A 41H Loop Names “Loop ”
B 42H Blank Line
C 43H Current Values “Cur:Value”
D 44H Operating Points “OP Points”
E 45H Output Percentage “Output % ”
J 4AH Control Modes “Control ”
K 4BH Blank Line
L 4CH Current Value Units Main Unit

M1226-0050 26
 Inquiries are acknowledged by the requested report in the format described below. If
parity is selected and a parity failure occurs, no response is made.

REPORT FORMAT
Each report begins with an identifying header (9 characters) followed by a space. The
identifier for each report is as shown in Table 3. The requested data for each installed
loop is then printed in a six character field followed by a space.

When no loop is installed, the data field for that loop is filled with spaces so that all
responses total 80 characters. Finally the response is terminated with a CR.

Figure 1.0e shows a typical response to a request for current values. The response is
shown in ASCII as well as the actual hexadecimal character string transmitted for
clarity. Similarly Figure 1.0c shows a typical response to a request for operating
points.

ONE DIRECTIVE IS SUPPORTED BY THE FERMENTOR

Modify Setpoints - Figure 1.0g shows the required format for a "Modify Set points"
directive. Note that the numeric data fields are positioned exactly the same as in a
report generated by the fermentor. The data field for each loop must contain a valid
ASCII numeric string (i.e., be composed of characters 0 thru 9 and may include a "+"
or "-" sign, a decimal point, and leading or trailing spaces) or may be filled with
spaces. A space-filled data field (20H 20H 20H 20H 20H 20H) for an installed loop
does not modify the set point of this loop. If parity has been selected and a parity
failure is detected in the directive, it will be ignored. The directive must be terminated
with a carriage return (0DH) after the 80th character. If the directive is received
properly the fermentor executes the command and acknowledges by sending a 06H.

M1226-0050 27
 TABLE 1
SETTINGS OF SWITCH 1 (S1) ON THE CONTROL BOARD

1. S1-2 and S1-1 are used for setting baud rate:

Baud Rate S1-2 S1-1

1200 ON ON
2400 ON OFF
4800 OFF ON
9600 OFF OFF

2. S1-3 is used for setting parity check:

Parity S1-3
even ON
no OFF

3. S1-4 is used for setting mode:

Mode S1-4
multidrop ON
non-multidrop OFF

4. S1-8, S1-7, S1-6, S1-5 are used for setting unit number:

Unit # S1-8 S1-7 S1-6 S1-5

0 ON ON ON ON
1 ON ON ON OFF
2 ON ON OFF ON
3 ON ON OFF OFF
4 ON OFF ON ON
5 ON OFF ON OFF
6 ON OFF OFF ON
7 ON OFF OFF OFF
8 OFF ON ON ON
9 OFF ON ON OFF
10 OFF ON OFF ON
11 OFF ON OFF OFF
12 OFF OFF ON ON
13 OFF OFF ON OFF
14 OFF OFF OFF ON
15 OFF OFF OFF OFF

M1226-0050 28
 TABLE 2
SERIAL DATA FORMAT

DATA FLOW

______________________________________________________________
star data data data data data stop stop
bit bit bit bit bit bit bit bit bit bit bit bit
0 1 2 3 4 5 6 7 8
_______________________________________________________________________
1 2 3 4 5 6 7 8 9 10 11 12

Mode and Parity bit 7 bit 8

__________________________________________________
multidrop, parity adata * parity bit
__________________________________________________
multidrop, no parity adata * 1 **
__________________________________________________
non-multidrop, parity parity bit 1 **
__________________________________________________
non-multidrop, no parity 0 ** 1 **
__________________________________________________

* adata - 1 indicates that the current date is a unit number.

- 0 indicates that the current date is a 7-bit data.

** This is the value the transmitter of Superboard sends out. The receiver of
Superboard does not care this bit.

M1226-0050 29
M1226-0050 30
 TABLE 4
SERIAL INTERFACE CONNECTOR

PIN # SIGNAL COMMENTS

1 NC

2 TXD RS232 Data Output from Fermentor

3 RXD RS232 Data Input to Fermentor

4 NC

5 NC

6 NC

7 GND Ground Reference for all Signal

8 NC

9 NC

10 NC

22 NC

11 NC

23 NC

12 IRXD+ RS422 Paired Data Input

24 IRXD- to Fermentor

13 ITXD+ RS422 Paired Data Output

25 ITXD- from Fermentor

14-19 NC

20 NC

21 IOS Open Selects RS232; Grounded Selects RS422

M1226-0050 31
 TABLE 5
HEXADECIMAL CODES
FOR
MULTIDROP UNIT NUMBERS

Unit Number Hexadecimal Code

0 80
1 81
2 82
3 83
4 84
5 85
6 86
7 87
8 88
9 89
10 8A
11 8B
12 8C
13 8D
14 8E
15 8F

M1226-0050 32
M1226-0050 33
 PARTS LIST - 1 1/4L, 2 1/2L AND 5L
BIOFLO III (Refer to Fig. 27A, 27B, 27C & 27D)

ITEM PART NUMBER DESCRIPTION QTY.

1a P0160-6450 Half Coupling 1

1b P0160-6450 Half Coupling 1
1c P0160-6451 Half Coupling 1
2a M1151-4100 Bearing Housing Assy. 1
2b M1152-4100 Bearing Housing Assy. 1
2c M1155-4100 Bearing Housing Assy. 1
3a P0280-5322 O-Ring 6
3b P0280-5322 O-Ring 7
3c P0280-5322 O-Ring 7
4a M1151-9412 Knob 3
4b M1151-9412 Knob 4
4c M1151-9412 Knob 4
5a P0280-8002 O-Ring 2
5b P0280-8062 O-Ring 2
5c P0280-8132 O-Ring 2
6a M1226-9204 Headplate 1
6b M1226-9206 Headplate 1
6c M1226-9208 Headplate 1
7a M1151-9905 Bumper, Flat Gasket 2
7b M1152-9904 Bumper, Flat Gasket 2
7c M1155-9902 Bumper, Flat Gasket 2
8a M1151-9437 Clamping Ring 1
8b M1226-9426 Clamping Ring 1
8c M1226-9432 Clamping Ring 1
*9 M1151-9436 Connector, Clamping Ring 4
10a M1151-9904 Glass Vessel 1
10b M1152-9903 Glass Vessel 1
10c M1155-9904 Glass Vessel 1
* 11 M1169-0610 Knob Screw 4
12a M1151-9435 Clamping Ring, Bottom 1
12b M1152-9412 Clamping Ring, Bottom 1
12c M1155-9434 Clamping Ring, Bottom 1
13a M1151-9201 Vessel Heater Assy. 1
13b M1152-9201 Vessel Heater Assy. 1
13c M1155-9206 Vessel Heater Assy. 1
* 14 P0240-0854 Quick Connect Body 1
* 15 P0240-0856 Quick Connect Body 1

ITEM PART NUMBER DESCRIPTION QTY.

M1226-0050 34
 16a M1151-9226 Baffle 1
16b M1152-9207 Baffle 1
16c M1155-9205 Baffle 1
17a M1151-9224 Impeller 2
17b M1152-9208 Impeller 2
17c M1155-9205 Impeller 2
* 18 M1151-9518 Plug, Glass Vessel 4
* 19 M1151-9520 Nut, Hose Connector 4
* 20 M1151-9419 Seal, Hose Connector 4
21a M1151-9227 Sparger Tube 1
21b M1152-9407 Sparger Tube 1
21c M1155-9203 Sparger Tube 1
22a M1226-9434 Collar Nut 1
22b M1151-9512 Collar Nut 1
22c M1151-9512 Collar Nut 1
* 23 M1151-9511 Plug, Inoc. Port 1
* 24 M1077-1040 Rubber, Syringe 1
* 25 M1226-9414 Plug 1/4” 3
* 26 M1132-946T Ferrule 1/4” 3
* 27 P0280-5302 O-Ring 4
* 28 M1132-9433 Plug, pH 1
* 29 M1132-9431 Cap 1
* 30 P0280-5912 O-Ring 2
* 31 M1226-9418 Plug 1/2” 1
* 32 H-727 Plug, DO 1
* 33 P0280-5932 O-Ring 1
* 34 M1176-9241 Plug, 3/8” 1
* 35 P0280-5342 O-Ring 1
* 36 M1226-9416 Plug 3/16” 1
* 37 M1226-9430 Ferrule 1
* 38 P0280-5292 O-Ring 1
* 39 S2217-2100 Cap Screw 8-32 x 5/8” 4
* 40 W2212-3088 Washer #8 Lock 4
* 41 S2114-3041 Screw 8-32 x 1/4” 8
* 42 W2217-3120 Washer 1/4” Lock 4
45a M1132-9144 Sampler Tube 1
45b M1132-9144 Sampler Tube 1
45c M1132-9141 Sampler Tube 1
* 46 M1151-9519 Hose Connector 4
47a M1151-9150 Nutrient Tube 2
47b M1152-9217 Nutrient Tube 2
47c M1155-9525 Nutrient Tube 2

ITEM PART NUMBER DESCRIPTION QTY.

M1226-0050 35
 48a M1151-940P Harvest Tube 1
48b M1152-9405 Harvest Tube 1
48c M1155-9416 Harvest Tube 1
49a M1132-9252 Well Thermister 1
49b M1132-9254 Well Thermister 1
49c M1132-9253 Well Thermister 1
* 50 P0280-5362 O-Ring 1
* 56 P0240-8270 Nut 1
* 57 P0240-7970 Ferrule 1
* 58 P0240-8260 Nut 1
* 59 P0240-7960 Ferrule 1
* 60 P0240-8240 Nut 3
* 61 P0240-7940 Ferrule 3
* 62 P0240-8230 Nut 1
* 63 P0240-7930 Ferrule 1

NOTES:
* indicates common to all three sizes
a indicates parts for 1 1/4L vessel
b indicates parts for 2 1/2L vessel
c indicates parts for 5L vessel

M1226-0050 36
 VESSEL SPARE PARTS
1.25L (M1226-0100)

PART NO. DESCRIPTION WHERE USED QTY

P0280-5322 O-Ring 2-010 EPRP For knob assy., 3 per headplate, 3 for ¼” ports 18
M1016-0890 Washer, D.O. Probe Used with probe adapter for 12mm probe 2
P0280-5912 O-Ring 2-111 EPRP On pH port (on top and bottom) 6
P0280-5292 O-Ring 2-007 EPRP When foam probe used 3
P0280-5302 O-Ring 2-008 EPRP On foam probe; harvest port; sampling port; antifoam port 12
P0280-5342 O-Ring 2-012 EPRP On thermowell port 3
P0280-5932 O-Ring 2-113 EPRP On D.O. port for 14mm probe 3
M1077-1040 Diaphragm On inoculation port 2
M1132-946T Ferrule On ¼” port assy. with tubing 6
M1226-9430 Ferrule 3/16” On 3/16” port assy. with foam probe 2
P0240-7960 Ferrule Frt SS 3/8 tube On water in/out jacket to console (item #2) flexible hose assy 2
P0160-6389 Coupling Sleeve On motor assy., connects to bearing housing 2
P0540-0150 Chipboard Packaging for all these parts 1
M1151-9419 Seal - Silicone On hose connector plugs on glass side, goes inside sampler assy. 6
P0280-5362 O-Ring 2-014 EPRP On condenser port 3
P0180-0170 Ball Bearing On bearing housing 3
P0280-5422 O-Ring 2-020 EPRP On cap of bearing housing 3
P0280-5472 O-Ring 2-025 EPRP On bearing housing 3
P0280-8002 O-Ring 2-353 EPRP On head plate and base plate 4
M1151-9905 Bumper Protects glass vessel 2

M1226-0050 37
 VESSEL SPARE PARTS
2.5L (M1226-0101)

PART NO. DESCRIPTION WHERE USED QTY

P0280-5322 O-Ring 2-010 EPRP For knob assy., 4 per headplate, 3 for ¼” ports 18
M1016-0890 Washer, D.O. Probe Used with probe adapter for 12mm probe 2
P0280-5912 O-Ring 2-111 EPRP On pH port (on top and bottom) 6
P0280-5292 O-Ring 2-007 EPRP When foam probe used 3
P0280-5302 O-Ring 2-008 EPRP On foam probe, harvest port, sampling port, antifoam port 12
P0280-5342 O-Ring 2-012 EPRP On thermowell port 3
P0280-5932 O-Ring 2-113 EPRP On D.O. port for 14mm probe 3
M1077-1040 Diaphragm On inoculation port 2
M1132-946T Ferrule On ¼” port assy. with tubing 6
M1226-9430 Ferrule 3/16” On 3/16” port assy. with foam probe 2
P0240-7960 Ferrule Frt SS 3/8 tube On water in/out jacket to console (item #2) flexible hose assy. 2
P0160-6389 Coupling Sleeve On motor assy., connects to bearing housing 2
M1151-9419 Seal-Silicone On hose connector plugs on glass side, goes inside sampler assy. 6
P0280-5362 O-Ring 2-014 EPRP On condenser port 3
P0180-0170 Ball Bearing On bearing housing 3
P0280-5422 O-Ring 2-020 EPRP On cap of bearing housing 3
P0280-5472 O-Ring 2-025 EPRP On bearing housing 3
P0280-8062 O-Ring 2-359 EPRP On head plate and base plate 4
M1152-9904 Bumper Protects glass vessel 2
P0540-0150 Chipboard Packaging for all these parts 1

M1226-0050 38
 VESSEL SPARE PARTS
5L (M1226-0102)

PART NO. DESCRIPTION WHERE USED QTY

P0280-5322 O-Ring 2-010 EPRP For knob assy., 4 per headplate, 3 for ¼” ports 18
M1016-0890 Washer, D.O. Probe Used with probe adapter for 12mm probe 2
P0280-5912 O-Ring 2-111 EPRP On pH port (on top and bottom) 6
P0280-5292 O-Ring 2-007 EPRP When foam probe used 3
P0280-5302 O-Ring 2-008 EPRP On foam probe, harvest port, sampling port, antifoam port 12
P0280-5342 O-Ring 2-012 EPRP On thermowell port 3
P0280-5932 O-Ring 2-113 EPRP On D.O. port 3
M1077-1040 Diaphragm On inoculation port 2
M1132-946T Ferrule On ¼” port assy with tubing 6
M1226-9430 Ferrule 3/16” On 3/16” port assy. with foam probe 2
P0240-7960 Ferrule Frt SS 3/8 tube On water in/out jacket to console (item #2) flexible hose assy. 2
P0160-6389 Coupling Sleeve On motor assy., connects to bearing housing 2
M1151-9419 Seal - Silicone On hose connector plugs on glass side, goes inside sampler assy. 6
P0280-5362 O-Ring 2-014 EPRP On condenser port 3
P0180-0170 Ball Bearing Bearing housing 3
P0280-5422 O-Ring 2-020 EPRP On cap of bearing housing 3
P0280-5472 O-Ring 2-025 EPRP On bearing housing 3
P0280-8132 O-Ring 2-366 EPRP On head plate and base plate 4
M1155-9902 Bumper Protects glass vessel 2
P0540-0150 Chipboard Packaging for all these parts 1

M1226-0050 39
 DRAWING LIST

Figure 1 Vessel Schematic

2 Sampling System
3 Flowmeter Conversion
4 Front Panel
5 Rear of Cabinet
6 Switch Panel
7 External Nutrient Pump Terminal
8 Suggested Impeller Location
9 Headplate
10 Sparger Tube
11 Harvest Tube
12 Thermowell
13 Foam Probe
14 Baffle Installation
15 Side Ports
16 pH Probe Installation
17 DO Probe (Phoenix) Installation
18 DO Probe (Ingold) Installation
19 Conversion of DO Port to Inoculation Port
20 Exhaust Condenser Installation
21 Restrictor in Condenser Water Line
22 Disassembly of Condenser
23 Factor Affecting pH Control
24A Control Schematic (100V)
24B Control Schematic (115V)
24C Control Schematic (220V)
25 Bearing Housing Assembly
26 Bearing Housing in Sterilization
27A Vessel Assembly
27B Headplate Assembly (1 1/4L, 2 1/2L, 5L)
27C Port Assembly II
27D Port Assembly I

M1226-0050 40
 FIGURE 1 - VESSEL SCHEMATIC

M1226-0050 41
 FIGURE 2 - SAMPLING SYSTEM

M1226-0050 42
 FIGURE 3 - FLOWMETER CONVERSION

M1226-0050 43
 FIGURE 4 - FRONT PANEL

M1226-0050 44
 FIGURE 5 - REAR OF CABINET

M1226-0050 45
 FIGURE 6 - SWITCH PANEL

M1226-0050 46
 FIGURE 7 - EXTERNAL NUTRIENT PUMP TERMINAL

M1226-0050 47
 FIGURE 8 - SUGGESTED IMPELLER LOCATION

M1226-0050 48
 FIGURE 9 - HEADPLATE

M1226-0050 49
 FIGURE 10 - SPARGER TUBE

M1226-0050 50
 FIGURE 11 - HARVEST TUBE

M1226-0050 51
 FIGURE 12 - THERMOWELL

M1226-0050 52
 FIGURE 13 - FOAM PROBE

M1226-0050 53
 FIGURE 14 - BAFFLE INSTALLATION

M1226-0050 54
 FIGURE 15 - SIDE PORTS

M1226-0050 55
 FIGURE 16 - pH PROBE INSTALLATION

M1226-0050 56
 FIGURE 17 - D.O. PROBE (PHOENIX) INSTALLATION

M1226-0050 57
 FIGURE 18 - D.O. PROBE (INGOLD) INSTALLATION

M1226-0050 58
 FIGURE 19 - CONVERSION OF D.O. PORT TO INOCULATION PORT

M1226-0050 59
 FIGURE 20 - EXHAUST CONDENSER INSTALLATION

M1226-0050 60
 FIGURE 21 - RESTRICTOR IN CONDENSER “WATER IN” LINE

M1226-0050 61
 FIGURE 22 - DISASSEMBLY OF CONDENSER

M1226-0050 62
 FIGURE 23 - FACTOR AFFECTING pH CONTROL

M1226-0050 63
M1226-0050
SSR001 2 GND 7
SAFETY TEMP. HEATER 001
1 3 +5V 10 3 GND 21
RS232C
SSR002 4 RXD 3
ACID NUTRIENT
FIGURE 24A-1 5 CTS 5 FIGURE 24A-2
PUMP PUMP 1 3
TO S009 6 IRXD 24
M M 7 IRXD 12
2 4 8 ICTS 11
COOL 13 J7 9 ICTS 23 J001
5 3 1 2 5 3 1 2 SWITCH 10 TXD 2 NUTRIENT
2 4 CONT. J1
15 11 RTS 4
5 HEATER CONT. 12 12 ITXD 13 SET POINT SPAN TEMPERATURE DO
3 ON 13 ITXD 25
J802 J801 CONTROL ZERO AGITATION pH
14 IRTS 10
P 15 IRTS 22
S006
OFF ACID
ACID MODE SELECTOR
J803
M AUTO 4

BASE
PUMP
ON 2 28 EX ADO 28
J10
6 27 EX AD1 27
1
5 5 26 EX AD2 26 1
S007
2 AC MOTOR OFF 25 EX AD3 25 2
DRIVE BASE BASE
24 EX AD4 24 3
P.C.B. J805 AUTO J3
5 23 EX AD5 23 4 GND
M1151-7008 6
3 22 EX AD6 22 5
TB004 SUPER NC
21 EX AD7 21 1 MODE S001
5 BOARD
ON M1226- 20 SW BKO 20 2
7000 19 DIS AND 19 J4 3
J804 11 18 DIS SEG 18 4 GND
M S008

PUMP
OFF NUTRIENT 11 SW BKI 11 5
NUTR NC

ANTIFOAM
17 +5 17
1 (3) AUTO (1) (2) J1
10 13 +5 13
J806
2 TB004 16 GND 16 DISPLAY
6 15 GND 15 BOARD
(9) 14 GND 14 M1153-
1 7 J3
ANTIFOAM AUTO 1 2 3 4 7 13 2 8 12 GND 12 7002
PROBE RTDI J4 10 GND 10 1
3 9 SELECTOR
(B) (A) GND RECEPTOR DO CONTROL
TB001 4 10 9 GND 9 2 S002
J1 NC
S005 0N (C) 4 5 11 8 GND 8 3
INCREASE
ANTIFOAM +5V 7 GND 7 4
J1-1 J2 DECREASE
TB005 5 6 GND 6 5
ANTIFOAM NC
DRIVER 5 GND 5 6
NC
M1151-4011 4 GND 4 7
J1-2 AGIT. ON
TB005 3 GND 3 8
3 NC
B TB001 (4) PH(H) M1169-7009 9 PH(H) 7 2 GND 2 9
E6 J003
A C PH(L) D.O./pH 8 PH(L) 8 1 GND 1
1 2 E7 J11
TB002 D PH SHIELD TRANSMITTER 7 +5V 6 1
A D E5
(3) 4 6 GND 5 4
D TB001 DO E3 J1
C (3) 3 4 D0(H) 7 2 3 3
J1-3 B C TB001 PROBE E1
E A 1 5 D0(L) 8 J12 3 4 pH 2
(2) C RECEPTOR E2
2 4 5 5
TB002 E4
SHIELD C A D A 5 6 DO
(1)
E3,E1: GALVANIC D.O. PROBE 6 7
B D 35mH
E2,E4: POLARGRAPHIC D.O. PROBE 7 8 AGIT.
P3 A+ 7
OFF ON 8 1
1 2 TEMP.
RECORDER SOCKET

S0010 1 KBIC 500mf

100 VAC P2 250v M TACH
POWER 2 J8 CONTROLLER
POWER
6 3 SUPPLY AGIT COMMAND 6
J5-7 J2-9 P1 A-
5
M1153-7000
19 TACH L1 L2
7 4
13 GND
M 110V
AC 12 +5V

PUMP
2 8 5 1 +5V 5
2 +5V 6
6

CIRCULATION
9 J4 6 GND 7
4 36000 fm J2
25V 7 GND 8
FIGURE 24A-3 J2
1 C003 7 11 PW DV 1 FIGURE 24A-4

LINE
IRF

LOAD
2 +15V 2

FILTER
T002 10 100JE4L
SWITCH BANK 1 A GND

P0460-1996
FOO1 8 6 3
BAUD RATE SI-1 SI-2 12A J3 10 -15V 4
1200 ON ON C 9 20 9
2400 ON OFF J6 TB003(1)
TB003(2)
C001 C002 J1 J5 C A
4800 OFF ON
9600 OFF OFF 10 A C B
B B A D
.1 m f .1 m f 3 2 6 4 2 C
SI-8 SI-7 SI-6 SI-5 OFF ON
ADDRESS FOO2
0 ON ON ON ON S0010 ADJ 2.5A
001

IN OUT
MOV

FLOO1
TB003(3)

1 ON ON ON OFF POWER LM338

2 ON ON OFF ON
3 ON ON OFF OFF (D) (B) F
4 ON OFF ON ON LINE A
5 ON OFF ON OFF (C) TB002 FILTER N
100 VAC
6 ON OFF OFF ON (2)
TB003(4)

7 ON OFF OFF OFF C A

(A) (C) TB002 (4)
8 OFF ON ON ON
9 OFF ON ON OFF B D
(B) (D)
10 OFF ON OFF ON

FIGURE 24A - CONTROL SCHEMATIC (100V)

11 OFF ON OFF OFF
12 OFF OFF ON ON VALVE
13 OFF OFF ON OFF
14 OFF OFF OFF ON ON 100 VAC
15 OFF OFF OFF OFF FROM
S009
SSR002 PRIME
SYSTEM S2-4 S2-3 S2-2 S2-1
BI0FL0 III ON ON ON ON
SWITCH SETTINGS OF S2

64
M1226-0050
SSR001
SAFETY TEMP. HEATER 001
1 3
SS
ACID NUTRIENT
PUMP PUMP 1
TO S009
M M
2 4

5 3 1 2 5 3 1 2 SWITCH
2
15
5 HEATE
ON
3
J802 J801
P
S006
OFF ACID
ACID
J803 AUTO
M

BASE
PUMP
ON
1
5
S007
2 AC MOTOR OFF
BASE BASE
DRIVE
5 P.C.B. J805 AUTO
M1151-7008 6
3 TB004
5
ON

J804 11
M S008

PUMP
OFF NUTRIENT
NUTR

ANTIFOAM
1 AUTO (1) (2)
10 (3)
J806
2 TB004
6
(9)

ANTIFOAM AUTO 1 2 3 4 7 13
PROBE RTD
(B) (A)

FIGURE 24A-1
GND RECEP
TB001 J1
S005 0N (C) 4
ANTIFOAM +5V
J1-1
TB005 5 ANTIFOAM
DRIVER
M1151-4011
J1-2
TB005 3
B TB001 (4) PH(H)
E6 M1169-7009
A C PH(L) D.O./pH
1 2 E7
TB002 D PH SHIELD TRANSMITTE
A D E5
(3) 4
D DO E3 J1
C TB001 (3) 3
J1-3 B C PROBE E1
E TB001
C A RECEPTOR 1
(2) E2
2
TB002 E4
SHIELD C A D A
(1)

B D E3,E1: GALVANIC
E2,E4: POLARGRAPHIC
OFF ON
1
S0010
POWER 100 VAC 2
POWER
6 3 SUPPLY
5 M1153-7010
1
7 4

65
M1226-0050
SSR001 2 GND 7
HEATER 001
1 3 +5V 10 3 GND 21
RS232C
SSR002 4 RXD 3
ENT
5 CTS 5
P 1 3
TO S009 6 IRXD 24
7 IRXD 12
2 4 8 ICTS 11

COOL 13 J7 9 ICTS 23 J001

1 2 SWITCH 10 TXD 2 NUTRIENT
2 4 CONT.
J1
15 11 RTS 4
HEATER CONT. 12 12 ITXD 13 SET POINT SPAN TEMPERATURE DO
ON 13 ITXD 25
1 CONTROL ZERO AGITATION pH
14 IRTS 10
P
S006 15 IRTS 22
OFF ACID
ACID MODE SELECTOR
AUTO 4
ON 2 28 EX ADO 28
J10 27 EX AD1 27
6
5 5 26 EX AD2 26 1
S007
OFF 25 EX AD3 25 2
BASE BASE
24 EX AD4 24 3
J805 AUTO J3
23 EX AD5 23 4 GND
6
22 EX AD6 22 5
TB004 SUPER NC
21 EX AD7 21 1 MODE S001
5 BOARD
ON 20 SW BKO 20 2
M1226-
7000 19 DIS AND 19 3
J4
11 18 DIS SEG 18 4 GND
S008
OFF NUTRIENT 11 SW BKI 11 5
NUTR NC
17 +5 17
AUTO (1) (2)
10 (3) 13 +5 13 J1

TB004 16 GND 16
DISPLAY
6 15 GND 15 BOARD
(9)
1 7 J3 14 GND 14 M1153-
7 13 2 8 12 GND 12 7002
RTDI 3 9 10 GND 10 1 SELECTOR
J4 DO CONTROL

FIGURE 24A-2
GND RECEPTOR
4 10 9 GND 9 2 S002
J1 NC
5 11 8 GND 8 3
INCREASE
+5V 7 GND 7 4
DECREASE
6 GND 6 J2 5
NC
5 GND 5 6
NC
4 GND 4 7
AGIT. ON
3 GND 3 8
NC
PH(H) 9 PH(H) 7 2 GND 2 9
E6 M1169-7009
PH(L) D.O./pH 8 PH(L) 8 1 GND 1 J003
E7 J11
PH SHIELD TRANSMITTER 7 +5V 6 1
E5 4
4 6 GND 5
DO E3 J1
3 4 D0(H) 7 2 3 3
PROBE E1
1 5 D0(L) 8 J12 3 4 pH 2
RECEPTOR E2
2 4 5 5
E4
5 6 DO
E3,E1: GALVANIC D.O. PROBE 6 7
7 8 AGIT. 35mH
E2,E4: POLARGRAPHIC D.O. PROBE 6
8 1 P3 A+
1 1 2 TEMP.
500mf
RECORDER SOCKET

KBIC
2
P2 M TACH
J8 CONTROLLER 250v
POWER
3 SUPPLY 7
P1 A-
M1153-7010
1 +5V 5 19 TACH L1 L2
4
13 GND

66
M1226-0050
(3) A D 4
D TB001 DO
C (3) 3
J1-3 B C TB001 PROBE
E A 1
(2) C RECEPTOR
TB002 2
SHIELD (1) C A D A

B D

OFF ON
S0010 1
POWER 100 VAC 2
POWER
6 3 SUPPLY
5 M1153-7000
7 4
M 110V
AC

PUMP
2 8 5
6

CIRCULATION
4 9 36000 fm J2
FIGURE 24A-3 25V
1 C003 7

LINE
IRF

LOAD

FILTER
T002 10 100JE4L
SWITCH BANK 1

P0460-1996
FOO1 8
BAUD RATE SI-1 SI-2 12A
1200 ON ON C 9
2400 ON OFF
C001 C002 J1 J5
4800 OFF ON
9600 OFF OFF 10

.1 m f .1 m f 3 2 6
SI-8 SI-7 SI-6 SI-5 OFF ON
ADDRESS
0 ON ON ON ON S0010 ADJ

FIGURE 24A-3
IN OUT
1 ON ON ON OFF POWER LM338
2 ON ON OFF ON
3 ON ON OFF OFF (D) (B)
4 ON OFF ON ON
5 ON OFF ON OFF (C) TB002
6 ON OFF OFF ON (2)
7 ON OFF OFF OFF
(A) (C) TB002 (4)
8 OFF ON ON ON
9 OFF ON ON OFF (B) (D)
10 OFF ON OFF ON
11 OFF ON OFF OFF
12 OFF OFF ON ON VALVE
13 OFF OFF ON OFF
14 OFF OFF OFF ON ON 100 VAC
15 OFF OFF OFF OFF FROM
S009
SSR002 PRIME
SYSTEM S2-4 S2-3 S2-2 S2-1
BI0FL0 III ON ON ON ON
SWITCH SETTINGS OF S2

67
M1226-0050
M1153-
7 13 2 8 12 GND 12 7002
RTDI 3 9 10 GND 10 1 SELECTOR
J4 DO CONTROL
GND RECEPTOR
4 10 9 GND 9 2 S002
J1 NC
5 11 8 GND 8 3
INCREASE
+5V 7 GND 7 4
DECREASE
6 GND 6 J2 5
NC
5 GND 5 6
NC
4 GND 4 7
AGIT. ON
3 GND 3 8
NC
PH(H) 9 PH(H) 7 2 GND 2 9
E6 M1169-7009
PH(L) D.O./pH 8 PH(L) 8 1 GND 1 J003
E7
PH SHIELD TRANSMITTER 7 +5V 6 J11 1
E5
4 6 GND 5 4
DO E3 J1
3 4 D0(H) 7 2 3 3
PROBE E1
1 5 D0(L) 8 J12 3 4 pH 2
RECEPTOR E2
2 4 5 5
E4
5 6 DO
E3,E1: GALVANIC D.O. PROBE 6 7
7 8 AGIT. 35mH
E2,E4: POLARGRAPHIC D.O. PROBE 6
8 1 P3 A+
1 1 2 TEMP.
KBIC 500mf

RECORDER SOCKET
P2 M TACH
2 J8 CONTROLLER 250v
POWER
3 SUPPLY 7
P1 A-
M1153-7010
1 +5V 5 19 TACH L1 L2
4
2 +5V 6 13 GND

J4 6 GND 7 COMMAND
5 J2-9
7 GND 8
6 J2
11 PW DV 1
36000
m f 2 +15V 2
J2
25V 6 A GND 3
C003 7
J3 10 -15V 4
20 9
8 J6

9
TB003(1)
TB003(2)
J1 J5 C A

FIGURE 24A-4
10 A C B
B B A D
mf 3 2 6 4 2
C
FOO2
2.5A
OUT
001
MOV

FLOO1
TB003(3)

F
LINE A
FILTER N
100 VAC
TB003(4)

C A
B D

68
M1226-0050
SSR001 2 GND 7
SAFETY TEMP. HEATER 001
FIGURE 24B-1 RED 1 3 +5V 10 3 GND 21 FIGURE 24B-2
RS232C
SSR002 4 RXD 3
ACID NUTRIENT
5 CTS 5
PUMP PUMP 1 3
TO S009 6 IRXD 24
M M 7 IRXD 12
2 4 8 ICTS 11
J7 9 ICTS 23 J001
COOL 13
5 3 1 2 5 3 1 2 SWITCH 10 TXD 2 NUTRIENT
2 4 CONT. J1
15 11 RTS 4
5 HEATER CONT. 12 12 ITXD 13 SET POINT SPAN TEMPERATURE DO
3 ON 13 ITXD 25
J802 J801 CONTROL ZERO AGITATION pH
14 IRTS 10
P 15 IRTS 22
S006
OFF ACID
ACID MODE SELECTOR
J803
M AUTO 4

BASE
PUMP
ON 2 28 EX ADO 28
J10 27 EX AD1 27
6
1
5 5 26 EX AD2 26 1
S007
2 AC MOTOR OFF 25 EX AD3 25 2
DRIVE BASE BASE
24 EX AD4 24 3
P.C.B. J805 AUTO J3
5 23 EX AD5 23 4 GND
M1151-7008 6
3 22 EX AD6 22 5
TB004 SUPER NC
21 EX AD7 21 1 MODE S001
5 BOARD
ON M1226- 20 SW BKO 20 2
7000 19 DIS AND 19 3
J804 J4
M 11 18 DIS SEG 18 4 GND
S008

VI0
PUMP
OFF 11 SW BKI 11 5
NUTR NUTRIENT NC

ANTIFOAM
17 +5 17
1 (3) AUTO (1) (2) J1
10 13 +5 13
J806
2 TB004 16 GND 16
DISPLAY
6 15 GND 15 BOARD
(9) J3 14 GND 14 M1153-
1 7
1 2 3 4 7 13 2 8 12 GND 12 7002
RTDI

RED
ANTIFOAM AUTO 3 9 J4 10 GND 10 1 SELECTOR
GND RECEPTOR DO CONTROL
PROBE 4 10 9 GND 9 2 S002
0RG (B) (A) J1 NC
TB001 5 11 8 GND 8 3
INCREASE
S005 0N (C) 4 +5V 7 GND 7 4
J2 DECREASE

BLK
ANTIFOAM 6 GND 6 5

BLK
NC

W\GRY
J1-1 5 GND 5
TB005 5 6
ANTIFOAM B TB001 (4) YEL NC
DRIVER 4 GND 4 7
A AGIT. ON
C 3 GND 3 8
M1151-4011 D NC
J1-2 PH(H) M1169-7009 9 PH(H) 7 2 GND 2 9
TB005 3 E6
PH(L) D.O./pH 8 PH(L) 8 1 GND 1 J003
TB002 E7 J11
PH SHIELD TRANSMITTER 7 +5V 6 1
(3) A D W/BLK 1 2 E5
4 6 GND 5 4
D TB001 DO E3 J1
B C C (3) 3 4 D0(H) 7 2 3 3
E TB001 PROBE E1
A RECEPTOR 1 5 D0(L) 8 J12 3 4 pH 2

VI0
J1-3 TB002 (2) C BLU E2
C A 2 4 5 5
(1) D A E4
5 6 DO
SHIELD B D E3,E1: GALVANIC D.O. PROBE 6 7
KBIC 35mH
E2,E4: POLARGRAPHIC D.O. PROBE 7 8 AGIT. CONTROLLER

W/BLK
RED
8 1 P3 A+
OFF ON 1 9 2 TEMP. + 7
RECORDER SOCKET

500mf
P2 M TACH

WHT
S0010 115 VAC 2 J8 250v

W/BLK
POWER POWER
6 3 BLK

BLK
SUPPLY AGIT COMMAND P1 A-
M1153-7000 J5-7 J2-9
5 19 TACH 6
L1 L2
7 4
115V 13 GND
12 +5V 8

WHT
AC +5V
2 8 5 1 5
6 2 +5V 6
4 9 36000 fm J4 6 GND 7
J2 GND
25V 7 8 J2
1 C003 7 11 PW DV 1

LINE
LOAD
FIGURE 24B-3 M IRF

FILTER
2 +15V 2 FIGURE 24B-4

PUMP
T002 10 100JE4L

P0460-1996
FOO1 8 6 A GND 3

CIRCULATION
SWITCH BANK 1 12A J3 10 -15V 4
BAUD RATE SI-1 SI-2 D
FL002 9 20 9 J6 TB003(1)
1200 ON ON J1 J5 TB003(2) W/RED
C001 C002 C A

BLU
2400 ON OFF 10 A C B
4800 OFF ON B B A D W/BLK BLU
9600 OFF OFF OFF ON .1 m f .1 m f 3 2 6 4 2 C
YEL

FOO2
S0010 ADJ 2.5A
001

IN OUT
MOV

ADDRESS SI-8 SI-7 SI-6 SI-5 POWER FLOO1

TB003(3)

LM338
0

BLK
ON ON ON ON
LINE W/BLK
1 ON ON ON OFF
(D) (B) BLK BLU FILTER F
2 ON ON OFF ON A
3 ON ON OFF OFF (C) TB002 115 VAC BLK N
LINE

LOAD
TB003(4)

4 ON OFF ON ON (2)
5 ON OFF ON OFF W/BLK (A) (C) TB002 (4) ORG C A VIO
6 ON OFF OFF ON B D
7 ON OFF OFF OFF (B) (D) BLK
8 OFF ON ON ON BLK
VALVE
9 OFF

FIGURE 24B - CONTROL SCHEMATIC (115V)

ON ON OFF
10 OFF ON OFF ON

BRN
11 OFF ON OFF OFF 115 VAC
BLU ON W/BLK
12 OFF OFF ON ON FROM
13 OFF OFF ON OFF
14 OFF OFF OFF ON BLU S009
15 OFF OFF OFF OFF SSR002 PRIME
WHT
W/BLK

SYSTEM S2-4 S2-3 S2-2 S2-1

BI0FL0 III ON ON ON ON
SWITCH SETTINGS OF S2

69
M1226-0050
SSR001
SAFETY TEMP. HEATER 001
1 3
SS
ACID NUTRIENT
PUMP PUMP 1
TO S009
M M
2 4

5 3 1 2 5 3 1 2 SWITCH
2
15
5 HEATE

3 ON
J802 J801
P
S006
OFF ACID
ACID
J803 AUTO
M

BASE
PUMP
ON
1
5
S007
2 AC MOTOR OFF
BASE BASE
DRIVE
5 P.C.B. J805 AUTO
M1151-7008 6
3 TB004
5
ON

J804 11
M S008

PUMP
OFF NUTRIENT
NUTR

ANTIFOAM
1 AUTO (1) (2)
10 (3)
J806
2 TB004
6
(9)

ANTIFOAM AUTO 1 2 3 4 7 13
PROBE RTD

FIGURE 24B-1
(B) (A) GND RECEPT
TB001 J1
S005 0N (C) 4

ANTIFOAM +5V
J1-1
TB005 5 ANTIFOAM
DRIVER
M1151-4011
J1-2
TB005 3
B TB001 (4) PH(H)
E6 M1169-7009
A C PH(L) D.O./pH
1 2 E7
TB002 D PH SHIELD TRANSMITTE
A D E5
(3) 4
D DO E3 J1
C TB001 (3) 3
J1-3 B C PROBE E1
E TB001
C A RECEPTOR 1
(2) E2
2
TB002 E4
SHIELD C A D A
(1)

B D E3,E1: GALVANIC
E2,E4: POLARGRAPHIC
OFF ON
1
S0010
POWER 115 VAC 2
POWER
6 3 SUPPLY AGIT
J5-7
5 M1153-7000
7 4
P
TION

70
M1226-0050
SSR001 2 GND 7
HEATER 001
1 3 +5V 10 3 GND 21
RS232C
SSR002 4 RXD 3
ENT
5 CTS 5
P 1 3
TO S009 6 IRXD 24
7 IRXD 12
2 4 8 ICTS 11
COOL 13 J7 9 ICTS 23 J001
1 2 SWITCH 10 TXD 2 NUTRIENT
2 4 CONT.
J1
15 11 RTS 4
HEATER CONT. 12 12 ITXD 13 SET POINT SPAN TEMPERATURE DO
ON 13 ITXD 25
1 CONTROL ZERO AGITATION pH
14 IRTS 10
P
S006 15 IRTS 22
OFF ACID
ACID MODE SELECTOR
AUTO 4
ON 2 28 EX ADO 28
J10 27 EX AD1 27
6
5 5 26 EX AD2 26 1
S007
OFF 25 EX AD3 25 2
BASE BASE
24 EX AD4 24 3
J805 AUTO J3
23 EX AD5 23 4 GND
6
22 EX AD6 22 5
TB004 SUPER NC
21 EX AD7 21 1 MODE S001
5 BOARD
ON 20 SW BKO 20 2
M1226-
7000 19 DIS AND 19 3
J4
11 18 DIS SEG 18 4 GND
S008
OFF NUTRIENT 11 SW BKI 11 5
NUTR NC
17 +5 17
AUTO (1) (2)
10 (3) 13 +5 13 J1

TB004 16 GND 16
DISPLAY
6 15 GND 15 BOARD
(9)
1 7 J3 14 GND 14 M1153-
7 13 2 8 12 GND 12 7002
RTDI 3 9 10 GND 10 1 SELECTOR
J4 DO CONTROL

FIGURE 24B-2
GND RECEPTOR
4 10 9 GND 9 2 S002
J1 NC
5 11 8 GND 8 3
INCREASE
+5V 7 GND 7 4
DECREASE
6 GND 6 J2 5
NC
5 GND 5 6
NC
4 GND 4 7
AGIT. ON
3 GND 3 8
NC
PH(H) 9 PH(H) 7 2 GND 2 9
E6 M1169-7009
PH(L) D.O./pH 8 PH(L) 8 1 GND 1 J003
E7 J11
PH SHIELD TRANSMITTER 7 +5V 6 1
E5 4
4 6 GND 5
DO E3 J1
3 4 D0(H) 7 2 3 3
PROBE E1
1 5 D0(L) 8 J12 3 4 pH 2
RECEPTOR E2
2 4 5 5
E4
5 6 DO
E3,E1: GALVANIC D.O. PROBE 6 7
7 8 AGIT. 35mH
E2,E4: POLARGRAPHIC D.O. PROBE 6
8 1 P3 A+
1 1 2 TEMP.
500mf
RECORDER SOCKET

KBIC
2
P2 M TACH
J8 CONTROLLER 250v
POWER
3 SUPPLY 7
AGIT COMMAND P1 A-
J5-7 J2-9
M1153-7000
19 TACH L1 L2
4

71
M1226-0050
D TB001 DO 4
B C C (3) 3
E TB001 PROBE
C BLU A RECEPTOR 1

VI0
J1-3 TB002 (2)
(1) C A 2
D A
SHIELD B D

W/BLK
OFF ON
1
S0010 115 VAC

WHT
2

W/BLK
POWER POWER
6 3 SUPPLY

BLK
M1153-7000 J5-7
5
7 4
115V

WHT
AC
2 8 5 1
6 2
4 9 36000 fm J4 6
J2 7
25V
1 C003 7 11

LINE
IRF

LOAD
FIGURE 24B-3 M 2

FILTER

PUMP
T002 10 100JE4L

P0460-1996
FOO1 8 6

CIRCULATION
SWITCH BANK 1 12A J3 10
BAUD RATE SI-1 SI-2 FL002 9 20
D
1200 ON ON J1 J5

BLU
C001 C002
2400 ON OFF 10
4800 OFF ON
9600 OFF OFF OFF ON .1 m f .1 m f 3 2 6 4 2

S0010 ADJ
ADDRESS SI-8 SI-7 SI-6 SI-5 POWER IN OUT
LM338
0 ON ON ON ON

BLK

FIGURE 24B-3
1 ON ON ON OFF
(D) (B) BLK
2 ON ON OFF ON
3 ON ON OFF OFF (C) TB002 115 VAC
4 ON OFF ON ON (2)
5 ON OFF ON OFF W/BLK (A) (C) TB002 (4)
6 ON OFF OFF ON
7 ON OFF OFF OFF (B) (D) BLK
8 OFF ON ON ON VALVE
9 OFF ON ON OFF
10 OFF ON OFF ON
BRN

11 OFF ON OFF OFF BLU ON 115 VAC

12 OFF OFF ON ON FROM
13 OFF OFF ON OFF
14 OFF OFF OFF ON BLU S009
15 OFF OFF OFF OFF SSR002 PRIME
WHT
W/BLK

SYSTEM S2-4 S2-3 S2-2 S2-1

BI0FL0 III ON ON ON ON
SWITCH SETTINGS OF S2

72
 M1153-

M1226-0050
7 13 2 8 12 GND 12 7002
RTDI 3 9 10 GND 10 1 SELECTOR
J4 DO CONTROL
GND RECEPTOR
4 10 9 GND 9 2 S002
J1 NC
5 11 8 GND 8 3
INCREASE
+5V 7 GND 7 4
DECREASE
6 GND 6 J2 5
NC
5 GND 5 6
NC
4 GND 4 7
AGIT. ON
3 GND 3 8
NC
PH(H) 9 PH(H) 7 2 GND 2 9
E6 M1169-7009
PH(L) D.O./pH 8 PH(L) 8 1 GND 1 J003
E7 J11
PH SHIELD TRANSMITTER 7 +5V 6 1
E5
4 6 GND 5 4
DO E3 J1
3 4 D0(H) 7 2 3 3
PROBE E1
1 5 D0(L) 8 J12 3 4 pH 2
RECEPTOR E2
2 4 5 5
E4
5 6 DO
E3,E1: GALVANIC D.O. PROBE 6 7
7 8 AGIT. 35mH
E2,E4: POLARGRAPHIC D.O. PROBE 6
8 1 P3 A+
1 1 2 TEMP.
500mf

RECORDER SOCKET
KBIC
2
P2 M TACH
J8 CONTROLLER 250v
POWER
3 SUPPLY 7
AGIT COMMAND P1 A-
M1153-7000 J5-7 J2-9
19 TACH L1 L2
4
13 GND
12 +5V
5 1 +5V 5
6 2 +5V 6
J4 6 GND 7
36000
m f J2 7 GND 8
25V J2
C003 7 11 PW DV 1
2 +15V 2
8 6 A GND 3
J3 10 -15V 4
9 20 9
J6 TB003(1)
TB003(2)
02 J1 J5 C A
10 A C

FIGURE 24B-4
B
B B A D
mf 3 2 6 4 2
C
FOO2
2.5A
OUT
001
MOV

FLOO1
TB003(3)

F
LINE A
FILTER N
115 VAC
TB003(4)

C A
B D

73
M1226-0050
SSR001 2 GND 7
SAFETY TEMP. HEATER 001
FIGURE 24C-1 RED 1 3 +5V 10 3 GND 21 FIGURE 24C-2
RS232C
SSR002 4 RXD 3
ACID NUTRIENT
5 CTS 5
PUMP PUMP 1 3
TO S009 6 IRXD 24
M M 7 IRXD 12
2 4 8 ICTS 11
J7 9 ICTS 23 J001
COOL 13
5 3 1 2 5 3 1 2 SWITCH 10 TXD 2 NUTRIENT
2 4 CONT. J1
15 11 RTS 4
5 HEATER CONT. 12 12 ITXD 13 SET POINT SPAN TEMPERATURE DO
3 ON 13 ITXD 25
J802 J801 CONTROL ZERO AGITATION pH
14 IRTS 10
P 15 IRTS 22
S006
OFF ACID
ACID MODE SELECTOR
J803
M AUTO 4

BASE
PUMP
ON 2 28 EX ADO 28
J10
6 27 EX AD1 27
1
5 5 26 EX AD2 26 1
S007
2 AC MOTOR OFF 25 EX AD3 25 2
DRIVE BASE BASE
24 EX AD4 24 3
P.C.B. J805 AUTO J3
5 23 EX AD5 23 4 GND
M1151-7008 6
3 22 EX AD6 22 5
TB004 SUPER NC
21 EX AD7 21 1 MODE S001
5 BOARD
ON M1226- 20 SW BKO 20 2
7000 19 DIS AND 19 3
J804 J4
M 11 18 DIS SEG 18 4 GND
S008

VI0
PUMP
OFF 11 SW BKI 11 5
NUTR NUTRIENT NC

ANTIFOAM
17 +5 17
1 (3) AUTO (1) (2) J1
10 13 +5 13
J806
2 TB004 16 GND 16
DISPLAY
6 15 GND 15 BOARD
(9) J3 14 GND 14 M1153-
1 7
1 2 3 4 7 13 2 8 12 GND 12 7002
RTDI

RED
ANTIFOAM AUTO 3 9 J4 10 GND 10 1 SELECTOR
GND RECEPTOR DO CONTROL
PROBE 4 10 9 GND 9 2 S002
0RG (B) (A) J1 NC
TB001 5 11 8 GND 8 3
INCREASE
S005 0N (C) 4 +5V 7 GND 7 4
J2 DECREASE

BLK
ANTIFOAM 6 GND 6 5

BLK
NC

W\GRY
J1-1 5 GND 5
TB005 5 6
ANTIFOAM B TB001 (4) YEL NC
DRIVER 4 GND 4 7
A AGIT. ON
C 3 GND 3 8
M1151-4011 D NC
J1-2 PH(H) M1169-7009 9 PH(H) 7 2 GND 2 9
TB005 3 E6
PH(L) D.O./pH 8 PH(L) 8 1 GND 1 J003
TB002 E7 J11
PH SHIELD TRANSMITTER 7 +5V 6 1
(3) A D W/BLK 1 2 E5
4 6 GND 5 4
D TB001 TB001 (7) DO E3 J1
B C C (3) 3 4 D0(H) 7 2 3 3
E TB001 PROBE E1
C A (A) (D) RECEPTOR 1 5 D0(L) 8 J12 3 4 pH 2
J1-3 TB002 (2) BLU E2
C A 2 4 5 5
(1) D A TB001 (8) E4
5 6 DO
SHIELD B D (A) (D) 10
E3,E1: GALVANIC D.O. PROBE 6 7
KBIC 35mH
E TB001 (5) E2,E4: POLARGRAPHIC D.O. PROBE 7 8 AGIT. CONTROLLER RED
8 1 P3 A+
OFF ON (A) (D) 0 7
9 2 TEMP. +
RECORDER SOCKET

500mf
1 P2 M TACH

WHT
S0010 J8 250v

W/BLK
POWER 220 VAC 2 BLK
POWER

BLK
J2-9 P1 A-
E 6 3 SUPPLY AGIT COMMAND 6
M1153-7000 J5-7 19 TACH L1 L2

WHT
5
7 4 13 GND
FOO3 12 +5V 8
6.25A 220 VAC
50 HZ +5V
2 8 5 1 5
6 2 +5V 6
9 J4 6 GND 7
4 36000 fm J2
J2 7 GND 8
25V

LINE
LOAD
FIGURE 24C-3 M 1 11 PW DV 1

FILTER
C003 7 FIGURE 24C-4

PUMP
IRF

P0460-1996
2 +15V 2
FOO1 T002 10 100JE4L
A GND

CIRCULATION
SWITCH BANK 1 6.25A 8 6 3
FL002 J3 10 -15V 4
BAUD RATE SI-1 SI-2 E TB003(1)
9 20 9 TB003(2) W/RED
1200 ON ON C A

BLU
J6
2400 ON OFF C001 C002 J1 J5 A C B
4800 OFF ON 10 B B A D W/BLK BLU
9600 OFF OFF OFF ON C
3 2 6 4 2
YEL

.1 m f .1 m f
FOO2
S0010 2.A
EXREF
001
MOV

ADDRESS SI-8 SI-7 SI-6 SI-5 POWER ADJ FLOO1

TB003(3)

IN OUT COMMAND
0 ON ON ON LM338

BLK
BLK

ON
BLU

EXGND LINE W/BLK

1 ON ON ON OFF
(D) (B) BLK BLU FILTER F
2 ON ON OFF ON A
3 ON ON OFF OFF (C) TB002 BLK N
LINE

LOAD

4 ON OFF ON
TB003(4)

ON (2) 220 VAC

5 ON OFF ON OFF W/BLK (A) (C) TB002 (4) ORG C A VIO
6 ON OFF OFF ON B D
7 ON OFF OFF OFF (B) (D) BLK
8 OFF ON ON ON BLK
VALVE

FIGURE 24C - CONTROL SCHEMATIC (220V)

9 OFF ON ON OFF
10 OFF ON OFF ON

BRN
11 OFF ON OFF OFF 220 VAC
BLU ON W/BLK
12 OFF OFF ON ON FROM
13 OFF OFF ON OFF
14 OFF OFF OFF ON BLU S009
15 OFF OFF OFF OFF SSR002 PRIME
WHT
W/BLK

SYSTEM S2-4 S2-3 S2-2 S2-1

BI0FL0 III ON ON ON ON
SWITCH SETTINGS OF S2

74
M1226-0050
SSR001
SAFETY TEMP. HEATER 001
1 3
SS
ACID NUTRIENT
PUMP PUMP 1
TO S009
M M
2 4

5 3 1 2 5 3 1 2 SWITCH
2
15
5 HEATE

3 ON
J802 J801
P
S006
OFF ACID
ACID
J803 AUTO
M

BASE
PUMP
ON
1
5
S007
2 AC MOTOR OFF
BASE BASE
DRIVE
5 P.C.B. J805 AUTO
M1151-7008 6
3 TB004
5
ON

J804 11
M S008

PUMP
OFF NUTRIENT
NUTR

ANTIFOAM
1 AUTO (1) (2)
10 (3)
J806
2 TB004
6
(9)

ANTIFOAM AUTO 1 2 3 4 7 13
PROBE RTD
(B) (A)

FIGURE 24C-1
GND RECEPT
TB001 J1
S005 0N (C) 4

ANTIFOAM +5V
J1-1
TB005 5 ANTIFOAM
DRIVER
M1151-4011
J1-2
TB005 3
B TB001 (4) PH(H)
E6 M1169-7009
A C PH(L) D.O./pH
1 2 E7
TB002 D PH SHIELD TRANSMITTE
A D E5
(3) 4
D TB001 (7) 220 DO E3 J1
C TB001 (3) 3
J1-3 B C PROBE E1
E TB001
C A (A) (D) RECEPTOR 1
(2) E2
TB001 (8) 2
TB002 E4
SHIELD C A D A
(1)
T001

(A) (D) 110

B D E3,E1: GALVANIC
TB001 (5) E2,E4: POLARGRAPHIC
OFF ON
(A) (D) 0
1
S0010
POWER 220 VAC 2
POWER
6 3 SUPPLY
M1169-7000 AGIT.
5 J5-7
7 4
P
TION

75
M1226-0050
SSR001 2 GND 7
HEATER 001
1 3 +5V 10 3 GND 21
RS232C
SSR002 4 RXD 3
ENT
5 CTS 5
P 1 3
TO S009 6 IRXD 24
7 IRXD 12
2 4 8 ICTS 11
COOL 13 J7 9 ICTS 23 J001
1 2 SWITCH 10 TXD 2 NUTRIENT
2 4 CONT.
J1
15 11 RTS 4
HEATER CONT. 12 12 ITXD 13 SET POINT SPAN TEMPERATURE DO
ON 13 ITXD 25
1 CONTROL ZERO AGITATION pH
14 IRTS 10
P
S006 15 IRTS 22
OFF ACID
ACID MODE SELECTOR
AUTO 4
ON 2 28 EX ADO 28
J10 27 EX AD1 27
6
5 5 26 EX AD2 26 1
S007
OFF 25 EX AD3 25 2
BASE BASE
24 EX AD4 24 3
J805 AUTO J3
23 EX AD5 23 4 GND
6
22 EX AD6 22 5
TB004 SUPER NC
21 EX AD7 21 1 MODE S001
5 BOARD
ON 20 SW BKO 20 2
M1226-
7000 19 DIS AND 19 3
J4
11 18 DIS SEG 18 4 GND
S008
OFF NUTRIENT 11 SW BKI 11 5
NUTR NC
17 +5 17
AUTO (1) (2)
10 (3) 13 +5 13 J1

TB004 16 GND 16
DISPLAY
6 15 GND 15 BOARD
(9)
1 7 J3 14 GND 14 M1153-
7 13 2 8 12 GND 12 7002
RTDI 3 9 10 GND 10 1 SELECTOR
J4 DO CONTROL

FIGURE 24C-2
GND RECEPTOR
4 10 9 GND 9 2 S002
J1 NC
5 11 8 GND 8 3
INCREASE
+5V 7 GND 7 4
DECREASE
6 GND 6 J2 5
NC
5 GND 5 6
NC
4 GND 4 7
AGIT. ON
3 GND 3 8
NC
PH(H) 9 PH(H) 7 2 GND 2 9
E6 M1169-7009
PH(L) D.O./pH 8 PH(L) 8 1 GND 1 J003
E7 J11
PH SHIELD TRANSMITTER 7 +5V 6 1
E5 4
4 6 GND 5
1 (7) 220 DO E3 J1
3 4 D0(H) 7 2 3 3
PROBE E1
(D) 1 5 D0(L) 8 J12 3 4 pH 2
RECEPTOR E2
2 4 5 5
1 (8) E4
5 6 DO

T001
(D) 110
E3,E1: GALVANIC D.O. PROBE 6 7
1 (5) 7 8 AGIT. 35mH
E2,E4: POLARGRAPHIC D.O. PROBE 6
8 1 P3 A+
(D) 0
1 1 2 TEMP.
500mf
RECORDER SOCKET

KBIC
2
P2 M TACH
J8 CONTROLLER 250v
POWER
3 SUPPLY 7
P1 A-
M1169-7000 AGIT. COMMAND
J5-7 J2-9 19 TACH L1 L2
4

76
 4

M1226-0050
D TB001 TB001 (7) DO
B C C (3) 3
E TB001 PROBE
C BLU A (A) (D) RECEPTOR 1
J1-3 TB002 (2)
(1) C A 2
D A TB001 (8)
SHIELD B D (A) (D) 10

E TB001 (5)
OFF ON (A) (D) 0

S0010 1

WHT
W/BLK
POWER 220 VAC 2
POWER

BLK
E 6 3 SUPPLY
M1153-7000

WHT
5
FOO3 7 4
6.25A 220 VAC
50 HZ
2 8 5
6
4 9 36000 fm J2
25V

LINE
LOAD
FIGURE 24C-3 M 1 C003 7

FILTER

PUMP
IRF

P0460-1996
FOO1 T002 10 100JE4L

CIRCULATION
SWITCH BANK 1 6.25A 8
BAUD RATE SI-1 SI-2 FL002
E
9
1200 ON ON

BLU
2400 ON OFF C001 C002 J1
4800 OFF ON 10
9600 OFF OFF OFF ON
.1 m f .1 m f 3 2 6
S0010
ADDRESS SI-8 SI-7 SI-6 SI-5 POWER ADJ
IN OUT
0 ON ON ON ON LM338

BLK
BLK
BLU

FIGURE 24C-3
1 ON ON ON OFF
(D) (B) BLK
2 ON ON OFF ON
3 ON ON OFF OFF (C) TB002
4 ON OFF ON ON (2)
5 ON OFF ON OFF W/BLK (A) (C) TB002 (4)
6 ON OFF OFF ON
7 ON OFF OFF OFF (B) (D) BLK
8 OFF ON ON ON VALVE
9 OFF ON ON OFF
10 OFF ON OFF ON
BRN

11 OFF ON OFF OFF BLU ON 220 VAC

12 OFF OFF ON ON FROM
13 OFF OFF ON OFF
14 OFF OFF OFF ON BLU S009
SSR002 PRIME
15 OFF OFF OFF OFF WHT
W/BLK

SYSTEM S2-4 S2-3 S2-2 S2-1

BI0FL0 III ON ON ON ON
SWITCH SETTINGS OF S2

77
 M1153-
7 13 2 8 12 GND 12 7002
RTDI 3 9 10 GND 10 1 SELECTOR
J4 DO CONTROL

M1226-0050
GND RECEPTOR
4 10 9 GND 9 2 S002
J1 NC
5 11 8 GND 8 3
INCREASE
+5V 7 GND 7 4
DECREASE
6 GND 6 J2 5
NC
5 GND 5 6
NC
4 GND 4 7
AGIT. ON
3 GND 3 8
NC
PH(H) 9 PH(H) 7 2 GND 2 9
E6 M1169-7009
PH(L) D.O./pH 8 PH(L) 8 1 GND 1 J003
E7 J11
PH SHIELD TRANSMITTER 7 +5V 6 1
E5
4 6 GND 5 4
1 (7) 220 DO E3 J1
3 4 D0(H) 7 2 3 3
PROBE E1
(D) 1 5 D0(L) 8 J12 3 4 pH 2
RECEPTOR E2
2 4 5 5
1 (8) E4
5 6 DO

T001
(D) 110 6 7
E3,E1: GALVANIC D.O. PROBE
1 (5) 7 8 AGIT. 35mH
E2,E4: POLARGRAPHIC D.O. PROBE 6
8 1 P3 A+
(D) 0
1 1 2 TEMP.

RECORDER SOCKET
KB 500mf
2
P2 M TACH
J8 CONTROLLER 250v
POWER
3 SUPPLY 7
P1 A-
M1169-7000 AGIT. COMMAND
J5-7 J2-9 19 TACH L1 L2
4
13 GND
12 +5V
5 1 5
+5V
6 2 +5V 6
36000
m f J4 6 GND 7
J2
25V 7 GND 8
7 J2
C003 11 PW DV 1
2 +15V 2
8 6 A GND 3
J3 10 -15V 4
9
20 9 TB003(1)
J6 TB003(2)
C002 J1 J5 C A

FIGURE 24C-4
10 A C B
B B A D
mf 3 2 6 4 2
EX REF C
FOO2
COMMAND 2A
OUT
001
MOV
TB003(3)

FLOO1
EX GND
F
A
LINE N
FILTER
220 VAC
TB003(4)

C A
B D

78
M1226-0050 79
 FIGURE 25 - BEARING HOUSING ASSEMBLY

M1226-0050 80
 FIGURE 26 - BEARING HOUSING IN STERILIZATION

M1226-0050 81
M1226-0050 82
M1226-0050 83
M1226-0050 84
M1226-0050 85