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Centrifuge 5424 R

Service manual - English

Copyright© 2015 All rights reserved, including graphics and images. No part of this publication may be
reproduced without the prior permission of the copyright owner.

Eppendorf® and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany.

CombiSlide® is registered trademarks of Eppendorf AG.

Centriplus® is a registered trademark of Millipore Corporation, Billerica, USA.

Microtainer® are registered trademarks of Becton Dickinson, Franklin Lakes, NJ, USA.

Registered trademarks and protected trademarks are not marked in all cases with ® or ™ in this manual.

5404 920.016-04/032015
 Table of contents
Centrifuge 5424 R 3
English (EN)

Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Hardware description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.1 PCB Display/Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.2 PCB Control/Converter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1.3 Drive and rotational speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1.4 Cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1.5 USB interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.2 Machine marking and serial number . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.1 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.2 Notes on liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.3 Safety notes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.4 Safety instructions on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.1 Overview of operating controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.1 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.1.1 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.2 Service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.2.1 Overview of functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.2.2 Activation and operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.2.3 Description of the service functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.3 Customer service functions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.3.1 Activation and operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.3.2 Lid latch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.3.3 Motor/electronic fan/intermediate circuit voltage . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.3.4 Frequency converter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.3.5 Temperature control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.3.6 Cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.3.7 Actual rotational speed/rotating field . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.3.8 Firmware versions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.3.9 Firmware checksum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.3.10 Error number/error cause . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
5.3.11 Serial number of the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
 Table of contents
4 Centrifuge 5424 R
English (EN)

6 Disassembly/assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
6.1 Disassembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
6.1.1 Centrifuge lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
6.1.2 Upper part of housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
6.1.3 PCB Display/Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
6.1.4 PCB Control/Converter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
6.1.5 Electronic fan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.1.6 PCB Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
6.1.7 PCB Lid Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.1.8 Lid latch motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.1.9 Reed switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
6.1.10 Motor and motor suspension . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
6.1.11 Brake resistor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
6.1.12 Condenser fan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
6.2 Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

7 Alignment/adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
7.1 General adjustments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
7.2 Adjustment of the reed switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

8 Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
8.1 Firmware . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
8.2 Software update . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

9 Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
9.1 Electronics Overview 5424_R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

10 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
10.1 Cleaning, disinfection, decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
10.2 Shipping the device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
10.3 Service and inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
10.3.1 Essential Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
10.3.2 Advanced Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
10.3.3 Qualification Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
10.3.4 Additional Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
10.4 Device tests for centrifuges. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
10.4.1 Speed check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
10.4.2 Temperature check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
10.4.3 Timer check. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
10.4.4 Electrical safety check. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
10.5 Interface test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

11 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
11.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
11.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
11.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
11.4 Application parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
11.4.1 Centrifuge 5424 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
11.5 Tolerances . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
 Table of contents
Centrifuge 5424 R 5
English (EN)

12 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
12.1 Images of spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
12.2 Spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
12.2.1 Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
12.2.2 Lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
12.2.3 Lid latch. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
12.2.4 Electronics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
12.2.5 Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
12.2.6 Fan. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
12.2.7 Cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
12.2.8 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
12.2.9 Rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
12.3 Suggested spare parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

13 Technical information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
 Table of contents
6 Centrifuge 5424 R
English (EN)
 Operating instructions
Centrifuge 5424 R 7
English (EN)

1 Operating instructions
1.1 Using this manual

 Make sure that the service manual and the operating manual are available in the latest versions. To do
so, compare the version numbers. The latest versions of the manuals can be downloaded from
www.eppendorf-support.com and www.eppendorf.com.
 Read the service manual before commencing work on the device.
 Read the chapters "Installation" and "Operation" of the operating manual.
 Observe the safety instructions in the operating manual.

1.2 Danger symbols and danger levels

The safety precautions in these instructions have the following danger symbols and danger levels.

1.2.1 Danger symbols

Biohazard Explosion

Electric shock Crushing

Cuts Hazard point

Material damage

1.2.2 Danger levels

DANGER Will lead to severe injuries or death.

WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.

1.3 Symbols used

Depiction Meaning
1. Actions in the specified order
2.
 Actions without a specified order
• List
Text Display text or software text
Additional information
 Operating instructions
8 Centrifuge 5424 R
English (EN)
 Product description
Centrifuge 5424 R 9
English (EN)

2 Product description
2.1 Hardware description

The electronics of the Centrifuge 5424 R consist of the following modules: PCB Display/Operation and PCB
Control/Converter.

The mains input module including switch and overcurrent protection devices is located on the right side of
the housing.

After the centrifuge is switched on, the hardware name and the firmware version of the control are
displayed shortly. The firmware can be updated via the USB interface.

2.1.1 PCB Display/Operation

This module mainly consists of control and input elements and an LC display. Its components and functions
are as follows:

• Input and output of centrifugation parameters, temperature, rotational speed, and time.
• Input and output (display by symbols) of all additional settings, e. g. "key lock", "At Set" function, etc.

There are two methods for entering centrifugation parameters:

• By rotary knob: Rotational speed and time are entered by means of two mechanical encoders; the
temperature is entered via arrow keys.
• By keypad: All centrifugation parameters are entered via arrow keys.

Further control and input elements:

• Start/Stop key
• Open key
• Rpm/Rcf key
• Menu/Enter key
• Menu arrow key
• Fast temp key
• Short key

For a detailed description of all control elements, please refer to the operating manual of the
device.
 Product description
10 Centrifuge 5424 R
English (EN)

2.1.2 PCB Control/Converter

This module controls and regulates all processes within the centrifuge. It is divided into two functional
areas: "Centrifuge control" and "drive & compressor control".

Centrifuge control
• Processing of signals received from the rotational speed, rotor bowl temperature, and condenser
temperature sensors.
• Control of all run parameters.
• Control of the electronics and condenser fans.
• Control of the solenoid valve for temperature control.
• Control of the speaker.
• Control of the lid-latch motor and recognition of the switching conditions of the individual lid switches.
The centrifuge control controls, via the driver circuit, the 12 Volt DC gear motor which locks and
unlocks the lid latch on the right and left sides. The locking condition is evaluated by means of a reed
switch (lid open/closed) and two microswitches (locking condition: locked/unlocked).

Drive and compressor control

• Interface for centrifuge control via optical coupler
• Switching power supply unit for 5 Volts and 12 Volts (floating), as well as for 5 Volts and 15 Volts
(energized).
• Radio interference filter.
• PFC circuit for 100 V, 120 V and 230 V.
For compliance with the EN 61 000-3-2 harmonics directive the voltage versions are equipped with an
active power factor correction (PFC) circuit essentially consisting of IC N25 (UCC 3818D), MOSFET V63
and the L10/ L32/ L33 PFC choke, which ensures that the line current drawn is almost sinusoidal.
Therefore, sufficient power is available for operating the drive and cooling even in the 100 V version.
• Braking chopper with external brake resistor.
• Module for control via centrifuge motor.
The intermediate circuit voltage and the intermediate circuit current are monitored.
• Module for control via compressor.
• Control of the electronics fan and the condenser fan.
The electronics fan is operated at different rotational speeds, depending on the cooling air required to
cool the centrifuge motor and the PCB Control/Converter.
The condenser fan is speed controlled as well, depending on the condenser temperature.
• Hardware safety circuit for lid switch and motor temperature safety switch outside of the processor
control.

When you switch the centrifuge off the current centrifuge parameters are stored in the PCB Control/
Converter. When you switch the centrifuge on again you can continue to operate with the centrifugation
parameters stored before.
 Product description
Centrifuge 5424 R 11
English (EN)

2.1.3 Drive and rotational speed

The drive motor used is a three-phase asynchronous motor.

Motor characteristics
• n = 15,000 rpm
• P = 200 Watts

The acceleration and the braking of the centrifuge motor are ramp-controlled.

The rotational speed of the motor is measured by a Hall sensor located on a separate printed circuit board
below the motor.

The rotational speed signal (one impulse per revolution) is used for rotational speed control and display.

2.1.4 Cooling

An NTC resistor embedded in a metal sleeve on the bottom of the motor chamber serves as motor chamber
temperature sensor.

The compressor is a rolling piston compressor which is operated at 55 Hz.

For optimum operation of the cooling system the condenser temperature is maintained in a range between
35 and 40 °C. For this purpose the rotational speed of the condenser fan is adjusted in 32 steps (step 32 is
the maximum rotational speed), depending on the condenser temperature.

The temperature is controlled via a bypass with a solenoid valve situated on the hot gas side.

The coolant is injected into the evaporator by a thermostatic injection valve.

2.1.5 USB interface

A USB interface is installed behind the cover on the left side of the device. The USB interface can be used
to update the firmware of the centrifuge if required (see Software update on p. 49).
 Product description
12 Centrifuge 5424 R
English (EN)

2.2 Machine marking and serial number

Abb. 2-1: Eppendorf AG machine marking using Centrifuge 5418 as an example

12 1

11 2

10 3

9 4

8 5

7 6

Fig. 2-1: Eppendorf AG machine marking using Centrifuge 5418 as an example

1 Manufacturer's address 7 CE mark

2 Consecutive number within the 12-digit serial 8 Power consumption
number
9 Permitted frequency
3 Maximum speed
10 Permitted voltage
4 Maximum kinetic energy
11 Product number
5 Maximum density of liquid
12 Manufacturer
6 Quality seal
 Safety
Centrifuge 5424 R 13
English (EN)

3 Safety
3.1 User profile

The specialist entrusted with maintenance, repair or testing must meet the following prerequisites:
• Successful participation in service trainings with certification by Eppendorf AG for the product that is to
be maintained, repaired or tested.
• Qualification as a specialist with knowledge of the applicable local and international standards.
• Qualification to evaluate the work assigned to him.
• Recognition of potential hazards and how to prevent or correct them.

3.2 Notes on liability

The Eppendorf service organization entrusted with maintenance, servicing or testing is liable for ensuring
that all work is carried out in a professional manner.

 Repair, maintenance and testing may not change the existing construction of the device. The device
must always be as safe as it is in its original state.
 Only accessories and original spare parts recommended by Eppendorf, and measuring and test
equipment recommended by Eppendorf may be used for any maintenance, servicing or testing work.

3.3 Safety notes

DANGER! Electric shock.

 Switch the device off.

 Unplug the power plug.
 Then begin repairing, servicing or cleaning the device.

DANGER! Risk of explosion.

 Do not operate the device in areas where work is completed with explosive substances.
 Do not use this device to process any explosive or highly reactive substances.
 Do not use this device for processing any substances which could generate an explosive
atmosphere.

WARNING! Infection by contaminated material.

There may be contaminated material on the device and accessories. Risk of infection with
contaminated material.

 Find out more about contamination risks before beginning work.

 Check the device decontamination certificate.
 Work may only be completed on a decontaminated device.
 Wear personal protective equipment (protective gloves, protective goggles).
 Safety
14 Centrifuge 5424 R
English (EN)

WARNING! Risk of injury when opening or closing the centrifuge lid.

There is a risk of crushing your fingers when opening or closing the centrifuge lid.

 When opening or closing the centrifuge lid, do not reach between the lid and device or into
the latching mechanism of the lid.
 Always open the centrifuge lid completely to prevent it from falling.

CAUTION! Injury from sharp-edged components.

The opened device contains sharp-edged components. Risk of injury from sharp-edged
components.

 Wear protective gloves.

 Work with extreme care.

NOTICE! Device damage due to unprofessional repairing of the refrigerating system.

 The refrigerating system must be repaired by a refrigeration specialist.

 Repairs, e.g. spare parts replacement, are carried out on one's own responsibility.
 Observe national and local safety regulations.

NOTICE! Damage to the compressor and refrigerating system due to tilting the device.
The compressor and refrigerating system can become damaged if the device is switched on
when it is tilted or if the device is switched on right after it was tilted.

 Leave the device in the normal position for at least 4 hours before switching it on.
 Never place the device upside down.

3.4 Safety instructions on the device

Symbol Meaning Location
Observe the operating manual. Right side of the device

CAUTION Top of device, under the

Always tighten the rotor with the enclosed rotor centrifuge lid.
key.
CAUTION Top of device, under the
Close all tubes and use a rotor lid. centrifuge lid.

Warning of biological risks when handling Aerosol-tight rotors/rotor lids,

infectious liquids or pathogenic germs. aerosol-tight buckets/caps.
 Operation
Centrifuge 5424 R 15
English (EN)

4 Operation
4.1 Overview of operating controls

The Centrifuge 5424 / 5424 R is available in two versions: keypad or rotary knobs. This operating manual
generally describes how to operate the keypad version. However, it also applies to the rotary knob version.

Before using the Centrifuge 5424 / 5424 R for the first time, familiarize yourself with the operating controls
and the display.

Abb. 4-1: Control panel of the Centrifuge 5424 / 5424 R

1 2 3 4 5

11 10 9 8 7 6

Fig. 4-1: Control panel of the Centrifuge 5424 / 5424 R

1 Call and select the menu parameters 7 Set centrifugation speed

Dependent on device version designed as a key or
dial.
2 Switch the displayed centrifugation speed
(rpm/rcf) 8 Adjust temperature (only 5424 R)
3 Display 9 Set centrifugation time
Dependent on device version designed as a key or
4 Short spin centrifugation
dial.
5 Release lid
10 Start the temperature control run fast temp (only
6 Start and stop centrifugation 5424 R)
11 Select the menu item
 Operation
16 Centrifuge 5424 R
English (EN)

Abb. 4-2: Display of the Centrifuge 5424 R, similar to the display of the Centrifuge 5424.

1 2 3 4 5 6 7

9 8

Fig. 4-2: Display of the Centrifuge 5424 R, similar to the display of the Centrifuge 5424.

1 Centrifugation time 6 g-force (rcf) or speed (rpm)

2 Status of the key lock (LOCK)
: Centrifugation parameters cannot be modified 7 Status of centrifuge
unintentionally. : Centrifuge lid unlocked.
: No key lock. : Centrifuge lid locked.
(flashing): Centrifuging in progress.
3 Status of ATSET function
: Start of run time when reaching 95% of the 8 Status of centrifugation speed display
preset g-force (rcf) or speed (rpm). rcf: g-force (relative centrifugal force)
: Immediate start of run time. rpm: revolutions per minute
4 Temperature (only 5424 R) 9 Status of speaker
: Switched on
5 Soft ramp
: Switched off
: Rotor accelerates and brakes slowly.
No symbol: fast acceleration and braking of rotor.
 Troubleshooting
Centrifuge 5424 R 17
English (EN)

5 Troubleshooting
5.1 Error messages

Error messages consist of a maximum of 3 parts:

• Error number (main error)

• Error cause
• Frequency converter (FC) error number

Up to firmware version 1.9: In case of errors, only the main error (e.g. Err 6) is displayed. To display the
complete error message (e.g. Err 6_2_4), press the rpm/rcf key for > 6 seconds. The display switches to
customer service function 9 (refer to “customer service functions" > “Error number / Error cause”). The
complete error message is displayed there. Customer service function 9 can only be accessed when an
error message is active. The FC error number is only relevant for error 6_2_xx. It is only displayed for this
error.

The complete error number is also displayed in service function SE 07.

From firmware version 2.0 upwards: The complete error message is displayed.

5.1.1 Error messages

Problem Cause Solution

Err 3_0 Rotational speed measuring system or  Keep the centrifuge switched on and wait
NO RPM drive until the idle period has elapsed.
• Error 3 occurs after mains supply has  Check the speed sensor (customer service
been activated. function 6).
(see Err 3_2)  Check motor connections.
 Replace motor.
 Replace PCB Control/Converter.
Err 3_1 Rotational speed measuring system or  Check the speed sensor (customer service
NO RPM drive function 6).
• No rotational speed impulses during  Check motor connections.
start.  Replace motor.
 Replace PCB Control/Converter.
Err 3_2 • No rotational speed impulses during  Keep the centrifuge switched on and wait
NO RPM run. until the idle period has elapsed.
Note: If this error occurs during the  Check the speed sensor (customer service
run, the centrifuge coasts to a stop function 6).
without braking and will remain  Check motor connections.
locked for up to 10 min. It cannot be  Replace motor.
operated and the lid cannot be  Replace PCB Control/Converter.
opened until the holding time has
elapsed. The error remains active
even after power off.
 Troubleshooting
18 Centrifuge 5424 R
English (EN)

Problem Cause Solution

Err 5_1 Lid latch  Check reed switch (service function SE 3).
Err 5_2 • The reed switch or microswitch signal  Replace reed switch.
failed during the run.  Check microswitch (service function SE
3).
 Replace PCB Lid Switch.
Err 5_3 Lid latch  Check microswitch (service function SE
• Signal from microswitch failed during 3).
run.  Replace PCB Lid Switch.
Err 6_1 Frequency converter (FC)  Replace PCB Control/Converter.
• No standby message after the
frequency converter has been
switched on.
Err 6_2-1 Frequency converter (FC)  Check the supply voltage. Connect the
• Low voltage. centrifuge to a different current circuit, if
required.
 Replace PCB Control/Converter.
Err 6_2-2 Frequency converter (FC)  Replace brake resistor.
• Overvoltage.  Replace PCB Control/Converter.
• Soldered fuse on brake resistor has
been triggered.
Err 6_2-3 Frequency converter (FC)  Allow the FC to cool down.
• Overheating of the FC.  Ensure sufficient ventilation. Clean the
ventilation slits, move the FC further away
from devices or walls.
 Check electronic fan (service function SE
12).
 Replace electronic fan.
Err 6_2-4 Frequency converter (FC)  Let the motor cool down.
• Overheating of the motor.  Check electronic fan and condenser fan
Temperature fuse in the motor has (service function SE 12).
been triggered.  Replace electronic fan or condenser fan.
 Replace motor.
Err 6_2-5 Frequency converter (FC)  Replace motor.
• Overcurrent.  Replace PCB Control/Converter.
Err 6_2-6 Frequency converter (FC)  Replace motor.
• Short circuit.  Replace PCB Control/Converter.
Err 6_2-7 Frequency converter (FC)  Check the lid switch.
• No release.  Replace PCB Lid Switch.
• Lid switches open.  Replace PCB Control/Converter.
Err 6_2-8 Frequency converter (FC)  Replace PCB Control/Converter.
• Internal reset.
• Times out.
Err 6_2-9 Frequency converter (FC)  Replace PCB Control/Converter.
• Internal FC error.
 Troubleshooting
Centrifuge 5424 R 19
English (EN)

Problem Cause Solution

Err 6_4 Frequency converter (FC)  Check the power supply voltage and
• The frequency converter has connect the centrifuge to a different
performed a reset. Power failure or current circuit if necessary.
power voltage too low.
Err 7_1 Speed control  Check the speed sensor (customer service
• The maximum speed was exceeded. function 6).
 Replace PCB Control/Converter.
 Replace motor.
Err 7_2 Speed control  Check the speed sensor (customer service
• Rotational speed deviation >800 rpm function 6).
from the set value.  Replace PCB Control/Converter.
 Replace motor.
Err 8_0 Acceleration ramp/rotational speed  Replace motor.
deviation  Replace PCB Control/Converter.
• The set rotational speed is not
reached within the specified time.
Err 8_2 Acceleration ramp/rotational speed  Tighten rotor.
deviation
• The rotor is loose.
Err 9_xx EEPROM write error  Initialize device (service function SE 2).
• Write error on EEPROM.  Replace PCB Control/Converter.
Err 10_2 EEPROM read error
Err 10_5 • Read error on EEPROM.
Err 10_3 Serial number  Enter the serial number of the device
• Serial number of the device was not (service function SE 14).
entered or is invalid.
Err 11 Software monitoring  Initialize device (service function SE 2).
• Watchdog reset.  Reinstall firmware.
 If the error occurs repeatedly, replace the
PCB Control/Converter.
Err 14 Checksum error  Reinstall firmware.
• Program memory error.  Replace PCB Control/Converter.
Err 16_xx Communication
Err 17_xx • Communication error on PCB Control/
Converter.
Err 18_1 Rotor bowl temperature  Check compressor and solenoid valve
• After the temperature has been (service function SE 11). Note: The
adjusted, the ±5 °C interval is left compressor and solenoid valve may only
again. be replaced by a refrigeration technology
specialist!
 Check the temperature sensor (customer
service function 4 and 5).
 Replace temperature sensor.
 Troubleshooting
20 Centrifuge 5424 R
English (EN)

Problem Cause Solution

Err 18_2 Rotor bowl temperature  Check compressor and solenoid valve
• The temperature in the rotor bowl is (service function SE 11). Note: The
more than 12 °C higher than the set compressor and solenoid valve may only
temperature. be replaced by a refrigeration technology
specialist!
 Check the temperature sensor (customer
service function 4 and 5).
 Replace temperature sensor.
Err 18_3 Rotor bowl temperature  Check to see if the compressor starts up
• Excess temperature in the rotor bowl, shortly after the centrifuge is switched on.
temperature in the rotor bowl >50 °C.  Check compressor and solenoid valve
(service function SE 11). Note: The
compressor and solenoid valve may only
be replaced by a refrigeration technology
specialist!
 Check the temperature sensor (customer
service function 4 and 5).
 Replace temperature sensor.
Err 19_1 Condenser temperature  Allow the condenser to cool down.
• Overheating of the condenser,  Clean the condenser.
condenser temperature >58 °C.  Clean and open air inlet in the housing.
• Condenser contaminated.  Ensure there is a sufficient amount of
• Air supply to the housing space between the condenser and other
contaminated or closed. devices or walls, etc.
• Distance to other devices or walls,  Check to see if the condenser fan
etc., is too small. accelerates when the cooling is switched
• Condenser fan defective. on.
• The temperature sensor is defective  Check condenser fan (service function SE
or is not properly attached to the 12).
condenser.  Replace condenser fan.
 Check the temperature sensor (customer
service function 4 and 5).
 Replace temperature sensor.
Err 20_0 Temperature sensor in the rotor bowl  Replace temperature sensor.
• Temperature sensor (NTC) in the
rotor bowl is defective. Measured
value is outside the range of
-21°C.......+65°C.
Err 21_1 Temperature sensor on the condenser  Replace temperature sensor.
• Temperature sensor (NTC) of the
condenser defective (the measured
value is outside the range of -10 °C...
+80 °C)
 Troubleshooting
Centrifuge 5424 R 21
English (EN)

Problem Cause Solution

Err 22_1 Electronic fan  Check electronic fan (service function SE
NO E-FAN • Electronic fan is not running. The 12).
rotational speed impulses are not  Replace electronic fan.
recognized.  Reinstall firmware.
The run is not interrupted. The error  Replace PCB Control/Converter.
is only displayed at standstill.
Err 24_2 Compressor control  Check plug connection to the compressor.
• Error in the frequency converter of  Check compressor (service function SE
the compressor. 11).
 Reinstall firmware.
 Replace PCB Control/Converter.
Err 24_3 Compressor control  Check compressor (service function SE
• Overcurrent to the compressor. 11).
 Replace compressor. Note: It may only be
replaced by a refrigeration technology
specialist.
Err 24_5 Compressor control  Check plug connection to the compressor.
• No stand-by message from the  Check compressor (service function SE
compressor. 11).
 Reinstall firmware.
 Replace PCB Control/Converter.
Err 25 Power supply  Open centrifuge lid. The error message
• Power failure during run. can only be acknowledged when the
centrifuge lid is open!
 Check the supply voltage. Connect the
centrifuge to a different current circuit, if
required.
 Replace mains input module.
 Replace PCB Control/Converter.
Err 30_1 Lid latch  Update the firmware to Version 1.5 or
LID ERROR • The centrifuge lid is not locked; higher.
microswitch "locked position" cannot  Check lid latch (service function SE 3).
be reached.  Replace PCB Lid Switch.
LID ERROR is indicated in the display.  Replace lid latch motor.
Err 30_2 Lid latch
LID ERROR • Centrifuge lid is not correctly
unlocked. Microswitch "unlocked
position" is not reached. Lid is open.
LID ERROR is indicated in the display.
Err 30_3 Lid latch  Update the firmware to Version 1.5 or
LID ERROR • The signal from the reed switch or higher.
microswitch "locked position" is  Check lid latch (service function SE 3).
missing when the rotor has stopped  Replace reed switch or PCB Lid Switches.
and the centrifuge lid is closed.
LID ERROR is indicated in the display.
 Troubleshooting
22 Centrifuge 5424 R
English (EN)

Problem Cause Solution

Err 30_4 Lid latch
LID ERROR • Lid is not completely unlocked.
"Unlocked pos." of the microswitch is
not reached. Lid is closed.
LID ERROR is indicated in the display.
Err 30_5 Lid latch
LID ERROR • "Locked pos." of the microswitch was
completely locked and is now open
again.
LID ERROR is indicated in the display.
Err 30_6 Lid latch  Check lid latch (service function SE 3).
LID LIFT • The lid remains on the bottom after  Replace lid springs
the open key has been pressed.
LID LIFT is indicated in the display.
Err 36 EEPROM initialization  Perform initialization (service function SE
(only if the • EEPROM not initialized. 2).
service
function is
activated)
INT Power supply  See Err 25.
• Power failure during run.
Note: Err 25 is displayed in the fault
memory.
OFF Power supply
• Displayed after power off. The length
of time that the control remains active
depends on the operating state of the
centrifuge (acceleration, brakes,
standstill).
No function Power supply  Check power supply.
• Power supply in the lab faulty.  Replace fuses.
• Fuses defective.  Replace mains input module.
• Power switch (mains input module)
defective.
No Interface  Check connection between PC/laptop and
communication • Connection between PC/laptop and device. Note: The PC/laptop and
via interface device faulty. centrifuge must be switched on in order to
(software • Driver not installed on PC/laptop. establish a connection.
update cannot • Interface defective.  Install driver on PC/laptop.
be completed).  Check interface using the "CefuComTest"
software.
 Replace PCB Interface.
 Troubleshooting
Centrifuge 5424 R 23
English (EN)

5.2 Service functions

5.2.1 Overview of functions

Program number Program function

SE 1 Measure acceleration and deceleration times
SE 2 Initialize EEPROM
SE 3 Check the lid latch
SE 4 Check the displays and keys
SE 5 Cyclical endurance test
SE 6 Display the error list
SE 7 Display last 10 error messages
SE 8 Display the operating hours and the number of centrifuge runs
SE 9 Check overspeed cut-off
SE 10 Display the last four runs
SE 11 Check the compressor and the solenoid valve
SE 12 Check fan
SE 13 Check interface
SE 14 Enter serial number of the device (from firmware version 2.0 upwards)

5.2.2 Activation and operation

Proceed as follows to activate and operate the service functions:

1. To activate the service functions, press the rpm/rcf key and simultaneously switch on the centrifuge
using the mains power switch. Keep the Rpm/Rcf key pressed until SE 1 appears in the display.
2. To select a service function, turn the time rotary knob or press the time+ / time- keys.
3. To activate a service function, press the Start/Stop key.
4. To exit a service function and return to the selection screen, press the short key.
5. Switch off centrifuge to exit the service functions.

5.2.3 Description of the service functions

In the drawings of the displays, placeholders (e.g., for values) are shown as lowercase letters.
Uppercase letters are shown in the display as illustrated.
 Troubleshooting
24 Centrifuge 5424 R
English (EN)

5.2.3.1 SE 1 (Measurement of the acceleration and deceleration times)

After activating SE 1, a short display test is performed. All segments and symbols are displayed
simultaneously. After that, the display advances automatically to Display 1.

Display 1: Now you can start the measurement run:

1. Close the centrifuge lid.
2. Press the Start/Stop key to start the
measurement run.
The centrifuge accelerates to maximum speed.
Then the acceleration time (Display 2) is
alternately displayed with the current run data
(Display 3).
Display 2: • bbb: Acceleration time in seconds x 10.
Note: The measurement is carried out at 97 %
of the maximum speed (=14,550 rpm).

Display 3: • b:bb: Run time

• cc: Current rotor bowl temperature
• ddddd: Current actual speed

 Press the Start/Stop key to stop the

measurement run.
The centrifuge decelerates to standstill. Then the
deceleration time will be displayed (display 4).
Display 4: • bbb: Deceleration time in seconds x 10

From firmware version 2.0 upwards

This function can only be started after a short cool-down period to protect the brake resistor.

 Pay attention to the countdown displayed in the display.

 Troubleshooting
Centrifuge 5424 R 25
English (EN)

5.2.3.2 SE 2 (Initializing of EEPROM)

Display 1: Key functions:

• Short key: Cancels and exits the service
function without initializing.
• Rpm/Rcf key: Executes initialization.
Display 2: – The standard display values are set and
displayed briefly (Display 2).
– The error memory and device settings will
be reset. The load changes and the
operating hours are maintained.
– The service function is exited automatically
after the initialization has been successfully
completed.
• menu/enter and rpm/rcf keys: Execute full
initialization.
The load changes, operating hours and the
serial number of the device will be reset in
addition to the standard initialization.

5.2.3.3 SE 3 (Check of lid latch)

Display 1: • b: Lid position/reed switch (0 = lid open, 1 = lid

closed)
• c: Microswitch - lid locked (0 = not operated,
1 = operated)
• d: Microswitch - lid unlocked (0 = not operated,
1 = operated)
• h: Cut-off reason
– 1 = Timeout
– 2 = Limit switch reached (operated)
– 3 = Overcurrent
– 4 = End control
Key functions:
• rpm/rcf key: The lid latch motor is driven
step-by-step.
After 5 to 10 steps, the lid is completely locked
and the display (c) switches from 0 to 1.
• Rpm/Rcf key: Unlocks the lid latch in one step
(prerequisite: b = 1 and c = 1).
• open key: Releases the lid latch from any
position.
• Menu/Enter arrow key: Locks the lid latch from
any position.
 Troubleshooting
26 Centrifuge 5424 R
English (EN)

5.2.3.4 SE 4 (Display test and key test)

A brief display test is conducted following activation: all segments and symbols are first displayed
individually and then simultaneously (display 1).
After a few seconds the display advances automatically to the key test (Display 2).

Display 1:

Display 2: • bbbbb: The key last pressed.

 Press all keys in succession.

Every key pressed is displayed as clear text. The
service function will be automatically exited after
the start/stop key has been pressed.

5.2.3.5 SE 5 (Cyclical endurance test)

Display 1: • bb: Time

• cc: Temperature
• ddddd: Rotational speed
Test procedure: 10 runs are performed, separated
by breaks of 2 minutes each, Followed by a longer
break.
After the first run has been started, the other starts
are carried out automatically.
The parameters can be modified (however, it is
recommended to perform the cycle endurance test
with the preset values).
The duration of the longer break can be set prior to
the initial start (display 2).
Display 2: • bb: Time of the longer break in periods (basic
setting: 10 minutes).
Key functions:
• Fast temp key: Changes the duration of the
longer break, circulating from 1 to 20 minutes
(only possible prior the start of the first run).
• Time+/Time- keys or rotary Time knob: Set the
run time.
• Temp+/Temp- keys: Set the temperature.
• Speed+/speed- keys or rotary Speed knob: Set
the rotational speed.
• start/stop key: Start the cyclic endurance test.
• Start/Stop key: By pressing this key you can
stop a run manually before the time preset has
elapsed.

You can only exit the service functions during an interval.

 Troubleshooting
Centrifuge 5424 R 27
English (EN)

5.2.3.6 SE 6 (Displaying the error list)

All error numbers are displayed one after the other, along with the frequency of their occurrence. The
display can be advanced forward using the start/stop key. The service function is exited after the last error
number.

From firmware version 2.0 upwards: After the general error messages, the possible error messages of the
frequency converter (FC) for the motor control and the compressor control are displayed separately.

Display: • Err: General error messages

• EMO: FC error messages for motor control (=
Er 6_2_xx)
• EKO: FC error messages for compressor control
(= Er 24_2_xx)
• bb: Error number
• ccc: Frequency (max. 256)

The error list can be deleted by performing an initialization (SE 2).

Up to firmware version 1.9 the display advances automatically every second. From firmware
version 2.0 upwards, the display can be advanced manually using the start/stop key.

5.2.3.7 SE 7 (Displaying the last 10 error messages)

The last 10 error messages, including the error cause and the frequency converter (FC) error number, if
applicable, are displayed in the order of their occurrence. The display can be advanced forward using the
start/stop key. The service function is exited after the last error message.

Display: • b = 1: Last error

b = 2: Second-to-last error
etc.
• cc: Error number
• dd: Error cause
• hh: FC error number

The error list can be deleted by performing an initialization (SE 2).

Up to firmware version 1.9 the display advances automatically every second. From firmware
version 2.0 upwards, the display can be advanced manually using the start/stop key.
 Troubleshooting
28 Centrifuge 5424 R
English (EN)

5.2.3.8 SE 8 (Displaying the operating hours and number of runs)

The operating hours and number of centrifuge runs are displayed consecutively. The display can be
advanced forward using the start/stop key. The service function is exited after the last display.

Display 1: • bbbbb: Operating hours

Display 2: • ccccc: Number of runs

The operating hours and the number of load changes can be deleted by performing a full
initialization (SE 2).

5.2.3.9 SE 9 (Testing the overspeed cut-off)

NOTICE! Damage to device caused by excessive speed.

The centrifuge should automatically switch off when the cut-off speed of the inserted rotor has
been reached.

 Find out more information about the maximum speed of the inserted rotor.
 Observe the check.
 If the centrifuge does not automatically switch off, disconnect the centrifuge from the
power supply.

The inspection must be carried out with rotor!

Display: 1. Close the centrifuge lid.

2. Press the Start/Stop key to start the test run.
The centrifuge accelerates to cut-off speed and
must switch off in conjunction with Error 7.

The service function may only be exited after the rotor has come to a standstill.
The device decelerates without braking (duration approx. 5 minutes). Alternatively, you can
switch the main power switch of the device off and back on to decelerate the device with
braking.
 Troubleshooting
Centrifuge 5424 R 29
English (EN)

5.2.3.10 SE 10 (Displaying the last four runs)

The errors (Display 1) and operating parameters (Display 2) of the last four runs are displayed
consecutively. The display can be advanced forward using the start/stop key. The service function is exited
after the last display.

Display 1: • b = 1: Last run

• b = 2: Second-to-last run
etc.
• cc: Error number
• dd: Error cause
• hh: FC error number
Display 2: • bb: Run time
• cc: Temperature
• ddddd: Rotational speed

Up to firmware version 1.9, the display advances automatically every second. From firmware
version 2.0 upwards, the display can be advanced manually using the start/stop key.

5.2.3.11 SE 11 (Testing the compressor and the solenoid valve)

The service function SE 11 has three displays. You can use Display 2 to test the proper functioning of the
compressor and the solenoid valve.
To continue switching the displays,
 Press the Menu/Enter key.

Display 1:

Display 2: • b: Compressor ready for operation (0 = not

ready, 1 = ready)
• c: Compressor status (0 = switched off,
1 = switched on)
• d: Solenoid valve status (0 = closed, 1 = open)
• h: Overcurrent counter (for shop test only)
Key functions:
• Rpm/Rcf key: Opens and closes the solenoid
valve.
• Fast temp key: Switches the compressor on
and off.
Display 3:

The functions in Display 1 and Display 3 are for shop tests only!
 Troubleshooting
30 Centrifuge 5424 R
English (EN)

5.2.3.12 SE 12 (Testing the fans)

Display: • b: Electronic fan (0 = switched off,

1 = switched on)
• c: Condenser fan (0 = switched off,
1 = switched on)
• d: Electronic fan level (0...8).
• h: Condenser fan level (0...32).
Key functions:
• Menu/Enter key: Switches the electronic fan on
and off.
• Menu/Enter arrow key: Switches the
condenser fan on and off.
• Temp+/Temp- keys: Sets the fan level of the
electronic fan.
• Speed+/Speed- keys or rotational Speed knob:
Set the fan level of the condenser fan.

5.2.3.13 SE 13 (Interface check)

Observe the descriptions of the interface check (see Interface test on p. 67) and "CefuComTest"
software on the www.eppendorf-support.com website. A password is required to access the
website.
Both devices need to be switched on in order to connect the centrifuge and the PC/laptop
using the USB cable.

Display 1: 1. Start the "CefuComTest" program on your

laptop/PC.
2. In the "CefuComTest" program, select the COM
port the centrifuge is connected to. Click
START.
3. Activate the interface check (SE 13) on the
centrifuge.
4. Press the start/stop key.
Data will be sent and received ("0x5A" will be
displayed in the "CefuComTest" program).
After the check has been completed successfully,
the display switches to display 2:
Display 2: 1. Click on STOP in the "CefuComTest" program.
2. Exit the service function.
3. Disconnect the USB connection.

"ERROR" will be displayed if the check was unsuccessful.

1. Check if the correct COM port is set in the "CefuComTest" program.
2. Check to see if the centrifuge and PC/laptop were switched on when the USB connection
was made.
Repeat the check.
 Troubleshooting
Centrifuge 5424 R 31
English (EN)

5.2.3.14 SE 14 (Enter serial number of device)

It is possible to enter the serial number from firmware version 2.0 upwards.
The serial number has 12 characters and cannot be shown on the display. Therefore, the serial
number is entered in 2 parts

Display 1: Enter serial number part 1:

• bbbbbb: Part 1 of the serial number
Key functions:
• time+ / time- keys or time rotary knob: Select
position of the serial number.
• speed+ / speed- keys or speed rotary knob: Set
value of the serial number digit.
• start/stop key: Switch forward to part 2.
The display changes for entering the second part of
the serial number.
Display 2: Enter serial number part 2:
• cccccc: Part 2 of the serial number
Key functions:
• time+ / time- keys or time rotary knob: Select
serial number position.
• speed+ / speed- keys or speed rotary knob: Set
value of the serial number place.
• start/stop key: Save the serial number.
The logic of the serial number is checked after it
has been entered. If the serial number has been
entered correctly, OK is displayed. The service
function is exited.
If the serial number has been entered incorrectly,
ERROR is displayed.

 If ERROR is displayed, repeat the entry and

correct the serial number.
 Troubleshooting
32 Centrifuge 5424 R
English (EN)

5.3 Customer service functions

5.3.1 Activation and operation

Proceed as follows to activate and operate the customer service functions:

1. To activate the customer service functions, press and hold the Rpm/Rcf key for more than 6 seconds.
2. To move on to the next display, press the Rpm/Rcf key briefly.
3. To exit the customer service functions, press and hold the Rpm/Rcf key for more than 6 seconds.
The customer service functions can also be selected when the centrifuge is running.
The functions of the Start/Stop and Open keys remain unchanged while the customer service
functions are activated. The centrifuge can be started and stopped. The lid can be closed and
opened.

5.3.2 Lid latch

• b: Reason for cut-off

– 1 - Timeout
– 2 - Limit switch reached within the specified
period
– 3 - Overcurrent cut-off
– 4 - End control
• c: Lid position/reed switch (0 = lid open, 1 = lid
closed)
• d: Microswitch - lid locked (0 = not operated, 1 =
operated)
• h: Microswitch - lid unlocked (0 = not operated,
1 = operated)
• mm: Machine status (only for shop test)
• uu: Drive mode (only for shop test)

5.3.3 Motor/electronic fan/intermediate circuit voltage

• bb: Motor temperature

• c: Electronic fan level (0...8)
• ddd: Intermediate circuit voltage

5.3.4 Frequency converter

• bb: Frequency converter / drive temperature

• cc: Frequency converter / compressor
temperature
• dddd: Frequency converter status (four-digit hex
number, for shop test only)
 Troubleshooting
Centrifuge 5424 R 33
English (EN)

5.3.5 Temperature control

• bb b: Mean control temperature x 10

• c: Solenoid valve (0 = closed / 1 = open)
• ddd: Current actual temperature value at the
temperature sensor in the rotor bowl x 10

5.3.6 Cooling

• bb: Condenser temperature

• cc: Condenser fan level
• d: Compressor status (0 = Off/1 = On)
• hhhh: Frequency converter / compressor status
(four-digit hex number, for shop test only)

5.3.7 Actual rotational speed/rotating field

• bbb: When the lid is open = actual rotational

speed (turn rotor by hand)
• bbb: When the centrifuge is running = rotating
field

5.3.8 Firmware versions

• b.b: Control firmware version

• c.c: Frequency converter / drive firmware
version
• d.d: Frequency converter / compressor firmware
version

5.3.9 Firmware checksum

• bbbb: Firmware checksum (four-digit hex

number)
 Troubleshooting
34 Centrifuge 5424 R
English (EN)

5.3.10 Error number/error cause

Up to Firmware version 1.9: This display can only be called up in case of errors.
From firmware version 2.0 upwards: If no error is active, "-- -- --" is displayed.

• bb: Error number

• cc: Error cause
• dd: Frequency converter error number

5.3.11 Serial number of the device

The serial number of the device is displayed from firmware version 2.0 upwards.
The serial number has 12 characters and cannot be shown on the display. Therefore, the serial
number is displayed in 2 parts.

• bbbbbb: Part 1 of the serial number

• cccccc: Part 2 of the serial number
 Disassembly/assembly
Centrifuge 5424 R 35
English (EN)

6 Disassembly/assembly
6.1 Disassembly

Tools
• Phillips screwdriver size 1 / 2
• Flat-blade screwdriver approx. 0.5 x 3
• Allen key 2.5 mm/5 mm (rotor key)
• Open-end wrench 5.5 / 8 / 10

6.1.1 Centrifuge lid

Abb. 6-1: Centrifuge lid

1
5 2
2

3
3

Fig. 6-1: Centrifuge lid

1 Lid covering 4 Lid springs (2 pcs.)

2 Metal lid 5 Lid axis
3 Lid covering mounting screws (M4 x 8; Allen 6 Lid guide bar
screws 2.5 mm; 4 pcs.)

1. Open the centrifuge.

2. Unscrew the mounting screws of the lid covering. Video
3. Remove lid covering. (Video Step 2)
4. Unhook the lid springs. Video
5. Remove the lid axle and lid guide bar. (Video Step 4)
6. Remove the metal lid and the lid spring upwards. (Video Step 4)

It must be ensured that the lid springs are mounted in the proper position.
 Disassembly/assembly
36 Centrifuge 5424 R
English (EN)

6.1.2 Upper part of housing

Prerequisites
The centrifuge lid has been removed.

Abb. 6-2: Upper part of housing / housing screws

3 1

2
2

Fig. 6-2: Upper part of housing / housing screws

1 Upper part of housing 3 rotor bowl seal

2 Housing screws (M4 x 12; Allen screws 2.5
mm; 4 pcs.)

1. Unscrew the housing screws. Video

2. Lift the upper part of the housing together with the rotor bowl seal until it can be placed next to the left
side of the lower part. Video
3. Disconnect the connection (ribbon cable) to the upper part of the housing. Video
When mounting the housing, ensure that the rotor bowl seal is properly placed over the edge
of the chamber, and that the upper part of the housing is placed in the lateral groove of the
seal.
 Disassembly/assembly
Centrifuge 5424 R 37
English (EN)

6.1.3 PCB Display/Operation

Prerequisites
The centrifuge lid and the upper part of housing have been removed.
Abb. 6-3: PCB Display/Operation

Fig. 6-3: PCB Display/Operation

Abb. 6-4: PCB Display/Operation, rear

Fig. 6-4: PCB Display/Operation, rear

1 PCB Display/Operation 2 Mounting screws of the PCB Display/Operation

(EJOT® 4x10, 5 pcs.)

1. Pull off both rotary knobs. Video

2. Unscrew the mounting screws from the PCB Display/Operation. Video
3. Carefully lift the PCB Display/Operation off to prevent the display, the button and the actuator from
being damaged. Video
 Disassembly/assembly
38 Centrifuge 5424 R
English (EN)

6.1.4 PCB Control/Converter

Prerequisites
The centrifuge lid and the upper part of housing have been removed.

In some cases, the software/firmware version of the PCB(s) does not match the current status.

 After replacing the printed circuit board(s) check the software version and update it, if
required.
Abb. 6-5: PCB Control/Converter

Fig. 6-5: PCB Control/Converter

1 PCB Control/Converter 3 Mounting screws of the PCB Control/Converter

(M4 x 10, 5 pcs.)
2 Mounting screws of the PCB Control/Converter
(M4 x 6 incl. lock washer, 5 pcs.) 4 Heat sink
 Disassembly/assembly
Centrifuge 5424 R 39
English (EN)

1. Loosen all connecting cables to the PCB Control/Converter. Video

2. Unscrew the mounting screws of the PCB Control/Converter. (Video Step 1)
3. Remove the PCB Control/Converter. (Video Step 1)
During assembly, insert all of the mounting screws first. Then tighten the mounting screws on
the heat sink to ensure good contact with the base plate.
From firmware version 2.0 upwards, enter the serial number of the device in service function
SE 14 after replacing the PCB Control/converter.

6.1.5 Electronic fan

Prerequisites
The centrifuge lid, the upper part of the housing and the PCB Control/Converter have been removed.

Abb. 6-6: Electronic fan

2 1

3
4 4
3

Fig. 6-6: Electronic fan

1 Electronic fan 3 Mounting screws of the fan holder (M4 x 8, 2 pcs.)

2 Fan holder 4 Mounting screws of the electronic fan (M3 x 16, 4 pcs.)

1. Unscrew the mounting screws from the fan holder. Video

2. Remove the electronic fan together with the fan holder. (Video Step 1)
3. Unscrew the electronic fan from the fan holder; note the mounting position.
4. Remove the connecting cable.
Make sure the proper mounting position is used during mounting. The ventilation flow must
be directed into the device.
 Disassembly/assembly
40 Centrifuge 5424 R
English (EN)

6.1.6 PCB Interface

Prerequisites
The centrifuge lid and the upper part of housing have been removed.

Abb. 6-7: PCB Interface

Fig. 6-7: PCB Interface

1 PCB Interface 2 Mounting screws of the PCB Interface (M4x6

incl. lock washer, 3 pcs.)

1. Loosen the connecting cable (ribbon cable) to the PCB Control/Converter. Video
2. Unscrew the mounting screws of the PCB Interface. (Video Step 1)
3. Remove the PCB Interface. (Video Step 1)
 Disassembly/assembly
Centrifuge 5424 R 41
English (EN)

6.1.7 PCB Lid Switch

Prerequisites
The centrifuge lid and the upper part of housing have been removed.

Abb. 6-8: PCB Lid Switch

Fig. 6-8: PCB Lid Switch

1 PCB Lid Switch 2 Mounting screws of the PCB Lid Switches

(M4 x 6 incl. lock washer, 2 pcs.)

1. Loosen the connecting cable to the PCB Control/Converter. Video

2. Unscrew the mounting screws of the PCB Lid Switches. (Video Step 1)
3. Remove the PCB Lid Switches. (Video Step 1)
 Disassembly/assembly
42 Centrifuge 5424 R
English (EN)

6.1.8 Lid latch motor

Prerequisites
The centrifuge lid, the upper part of the housing, the PCB Lid Switches and the motor have been removed.

Abb. 6-9: Lid latch motor

5 3
6

1
2

Fig. 6-9: Lid latch motor

1 Lid latch motor 4 Latch hook

2 Mounting screws of the lid latch motor (M3 x 8, 5 Guiding washer
3 pcs.)
6 Gear wheel segment
3 Latch hook nut (M5, 8 mm)

1. Loosen the connecting cable between the lid latch motor and the PCB Control/Converter. Video
2. Unscrew the nut from the latch hook and remove the latch hook. (Video Step 1)
3. Remove the gear segment and guiding washer. (Video Step 1)
4. Unscrew the assembly screw from the lid latch motor. (Video Step 1)
5. Remove lid latch motor. (Video Step 1)
 Disassembly/assembly
Centrifuge 5424 R 43
English (EN)

6.1.9 Reed switch

Prerequisites
The centrifuge lid and the upper part of housing have been removed.

Abb. 6-10: Reed switch

Fig. 6-10: Reed switch

1 Reed switch 2 Mounting screws of the reed switch (M3 x 10,

locked by a lock washer and nut ( 5.5 mm),
2 pcs.)

1. Loosen the connecting cable to the PCB Control/Converter.

2. Unscrew the mounting screws from the reed switch.
3. Remove the reed switch.
When mounting the reed switch observe the instructions regarding adjustment (see
Adjustment of the reed switch on p. 47).
 Disassembly/assembly
44 Centrifuge 5424 R
English (EN)

6.1.10 Motor and motor suspension

NOTICE! Damage to the compressor and refrigerating system due to tilting the device.
The compressor and refrigerating system can become damaged if the device is switched on
when it is tilted or if the device is switched on right after it was tilted.

 Leave the device in the normal position for at least 4 hours before switching it on.
 Never place the device upside down.

Prerequisites
The motor can be changed without disassembling the housing. The centrifuge lid and the upper part of the
housing do not need to be removed.

Abb. 6-11: Motor and motor suspension

2 2

1 4 5

Fig. 6-11: Motor and motor suspension

1 Motor base plate 4 Shock mounts (motor suspension) with

sandpaper disks
2 Mounting screws of the motor base plate
(M4 x 12, 6 pcs.) 5 Nuts of the shock mounts (M6, 10 mm, 3 pcs.)
3 Motor 6 Motor plate sleeve

1. Open the centrifuge lid and remove the rotor.

2. Place the centrifuge on the left side. Video
3. Unscrew the mounting screws of the motor base plate. (Video Step 2)
4. Remove the motor including motor base plate. (Video Step 2)
5. Loosen the connecting cable to the motor and the ground strap. (Video Step 2)
 Disassembly/assembly
Centrifuge 5424 R 45
English (EN)

6. Loosen the nuts of the shock mounts (motor suspension). Video

7. Remove the motor in an upward motion. (Video Step 6)
When mounting the motor ensure that the motor sealing is completely in the groove of the
motor plate sleeve.
The sandpaper disks serve as an anti-twist lock. When mounting the motor, the nuts on the
sides with the sandpaper disks should be tightened last to prevent the rubber sleeve from
being twisted.

6.1.11 Brake resistor

Prerequisites
The centrifuge lid and the upper part of housing have been removed.

The desoldering fuse on the brake resistor may not be resoldered! If an error occurs, the
behavior of the fuse cannot be predicted if the type and quantity of the solder, and the
soldering temperature, are not an exact match.

 The entire brake resistor always needs to be replaced if the soldering fuse is open.
Abb. 6-12: Brake resistor

Fig. 6-12: Brake resistor

1 Brake resistor 3 Mounting screw of the retaining bracket

(M4 x 6, 1 piece)
2 Angle bracket

1. Loosen the connecting cable to the PCB Control/Converter. Video

2. Unscrew the mounting screw from the retaining bracket. Video
3. Remove the brake resistor together with the retaining bracket. (Video Step 2)
 Disassembly/assembly
46 Centrifuge 5424 R
English (EN)

6.1.12 Condenser fan

Prerequisites
The centrifuge lid, the upper part of the housing and the brake resistor have been removed.

Abb. 6-13: Condenser fan

Fig. 6-13: Condenser fan

1 Condenser fan 3 Mounting screws of the fan holder (M4 x 8,

2 pcs.)
2 Fan holder

1. Loosen the connecting cable to the PCB Control/Converter. Video

2. Unscrew the mounting screws from the fan holder. Video
3. Remove the condenser fan together with the fan holder in an upwards and backwards motion. (Video
Step 2)
4. Unscrew the condenser fan from the fan holder. Note the mounting position.
5. Remove the connecting cable.
Make sure the proper mounting position is used during mounting. The ventilation flow must
be directed to the compressor.

6.2 Assembly

For all assembly steps, the installation proceeds in reverse order of disassembly. Also read Chapter 7
"Adjustment" and Chapter 9 "Circuit diagrams" when performing assembly work.
 Alignment/adjustment
Centrifuge 5424 R 47
English (EN)

7 Alignment/adjustment
7.1 General adjustments

No adjustment of individual assemblies is required. Pay attention to the correct position of the cables and
free movement of the moving parts and assemblies. Make sure that the individual parts and assemblies
match.

The speed, temperature and time parameters as well as the safety arrangements have prescribed control
installations and thresholds that cannot be changed. It is not possible to adjust these parameters and
functions.

From firmware version 2.0 upwards, the serial number of the device must be entered in service function SE
14 after replacing the PCB Control/converter.

7.2 Adjustment of the reed switch

If the reed switch is mounted too low the lid latch may not be started when the centrifuge lid is
closed, resulting in the centrifuge lid not being locked.
If the reed switch is mounted too high the latch hooks may not reach the centrifuge lid,
resulting in the centrifuge lid not being locked.

Proceed as follows to adjust the reed switch:

 Mount the reed switch rather in the middle.

 Check the proper functioning of the lid latch with the housing and the centrifuge lid being mounted.
Correct the mounting position of the reed switch if required to ensure the proper functioning of the lid
latch.
 Alignment/adjustment
48 Centrifuge 5424 R
English (EN)
 Software
Centrifuge 5424 R 49
English (EN)

8 Software
8.1 Firmware

From firmware version 2.0 upwards, the serial number of the device must be entered in the service function
SE 14 after replacing the PCB Control/Converter.

8.2 Software update

In some cases, the software/firmware version of the PCB(s) does not match the current status.

 After replacing the printed circuit board(s) check the software version and update it, if
required.

The centrifuge firmware can be updated using a USB interface and a laptop/PC.

The interface is on the left-hand side of the device, behind the cover.

To ensure communication between the centrifuge and your laptop/PC you must install the
"CefuProgrammer" software (from version 1.1 on) and the drivers required on your laptop/PC.

The CefuProgrammer, driver, and instructions can be downloaded from the www.eppendorf-support.com
website (password required).

The current version of the firmware can also be downloaded from the www.eppendorf-support.com
website.

 Read the installation and operating instructions for the CefuProgrammer and the firmware
carefully before downloading, installing or updating the software!
 Software
50 Centrifuge 5424 R
English (EN)
 Diagrams
Centrifuge 5424 R 51
English (EN)

9 Diagrams
9.1 Electronics Overview 5424_R
 Diagrams
52 Centrifuge 5424 R
English (EN)
 Maintenance
Centrifuge 5424 R 53
English (EN)

10 Maintenance
10.1 Cleaning, disinfection, decontamination

DANGER! Electric shock as a result of penetration of liquid.

 Switch off the device and disconnect the power plug before starting cleaning or
disinfection work.
 Do not allow any liquids to penetrate the inside of the housing.
 Do not spray clean/spray disinfect the housing.
 Only plug the device back in if it is completely dry, both inside and outside.

WARNING! Infection by contaminated material.

There may be contaminated material on the device and accessories. Risk of infection with
contaminated material.

 Find out more about contamination risks before beginning work.

 Check the device decontamination certificate.
 Work may only be completed on a decontaminated device.
 Wear personal protective equipment (protective gloves, protective goggles).

NOTICE! Damage from the use of aggressive chemicals.

 Do not use any aggressive chemicals on the device or its accessories, such as strong and
weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol.
 If the device has been contaminated by aggressive chemicals, immediately clean it by
means of a mild cleaning agent.

NOTICE! Corrosion from aggressive cleaning agents and disinfectants.

 Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes.

 Do not incubate the accessories in aggressive cleaning agents or disinfectants for a longer
period of time.

For further information as well as a detailed description on how to perform cleaning,

disinfection and decontamination, please refer to the operating manual of the device.
Carefully read the respective sections of the operating manual before you start cleaning,
disinfection or decontamination.
 Maintenance
54 Centrifuge 5424 R
English (EN)

10.2 Shipping the device

WARNING! Risk to health from contaminated device

1. Follow the instructions in the decontamination certificate. You find it as a PDF file on our
website (www.eppendorf.com/decontamination).
2. Decontaminate all the parts you would like to dispatch.
3. Include the fully completed decontamination certificate in the packing.

NOTICE! Damage as a result of incorrect packing.

Eppendorf AG is not liable for damage caused by improper packing.

 The device may only be stored and transported in its original packing.

Transport packing can be ordered via your Eppendorf service organization. The order number
can be found in the chapter Ordering information.

10.3 Service and inspection

Eppendorf offers maintenance services for centrifuges in the form of Performance Plans.

Performance Plans for centrifuges are available with the following levels:
• Essential Check
• Advanced Maintenance
• Qualification Service
• Additional Maintenance

For each Performance Plan level the maintenance services are clearly defined. This chapter shows which
maintenance services are defined and how much time needs to be scheduled in for them. All maintenance
services are described.
 Maintenance
Centrifuge 5424 R 55
English (EN)

10.3.1 Essential Check

Number Verify Description

EC1 Checking the device for any damage.  Check the outside of the device for any damage.
EC2 Cleaning the device.  Clean the device on the outside as described in
the operating manual.
EC3 Refrigerated centrifuges  Empty the condensation water tray and clean it.
• Checking the condensation water tray. (Not for Centrifuge 5920 R.)
EC4 Checking the lid latch.  Check that the lid latch the lid properly locked.
EC5 Checking the rotor detection. Check all of the available rotors.

 Insert the rotor.

 Check whether the centrifuge detects the rotor.
EC6 Performing a test run.  Insert rotor with accessories.
 Charge the rotor with the maximum number of
filled tubes.
 Only refrigerated centrifuges: Start run with key
fast temp.
The temperature of 6 °C should be achieved quickly
(depending on rotor, note lowest achievable
temperature).

Time required: 0.25 h – 0.5 h per device

 56

Group 1 Group 2 Group 3 Group 4

Non-refrigerated Refrigerated Non-refrigerated Refrigerated
No imbalance Imbalance Imbalance Imbalance

10.3.2
detection detection detection detection
Advanced Maintenance for Centrifuges
English (EN)
Maintenance

5418
5424
5452
5453
5430
5702
5804
5810

5415 R
5418 R
5424 R
5430 R
5920 R
Centrifuge 5424 R

5417 R 5427 R
5702 R 5702 RH
5804 R 5810 R
Nr. Service interval in month 12 12 12 12 12 12 12 12 12 12 12 12 12 12 12 12
AM 01 Cleaning the device x x x x x x x x x x x x x x x x
AM 02 Cleaning the condensation water tray and the hose x x x x x x x x
AM 03 Checking the display x x x x x x x x x x x x x x x x
AM 04 Checking the keypad x x x x x x x x x x x x x x x x
Advanced Maintenance

AM 05 Checking the stability and the housing x x x x x x x x x x x x x x x x

AM 06 Checking the rotor bowl x x x x x x x x x x x x x x x x
AM 07 Cleaning and greasing the rotor bowl seal x x x x x x x x x x x x
AM 08 Checking latch mechanism, lid latch and emergency release x x x x x x x x x x x x x x x x
AM 09 Checking the lid springs, the gas springs and the lid shaft x x x x x x x x x x x x x x
AM 10 Cleaning and greasing the lid latch x x x x x x x x x x x x x x x x
AM 11 Checking the motor cap and the speed sensor x x x x x x x x
AM 12 Checking the motor sleeve x x x x x x x x
AM 13 Checking the motor and the motor shaft x x x x x x x x x x x x x x x x
AM 14 Checking the motor suspension x x x x x x x x x x x x x x x x
AM 15 Checking the imbalance detection x x x x x x x x x
AM 16 Checking motor fan, device fan and ventilation slots x x x x x x x x x x x x x x
AM 17 Checking the fuse holder x x x x x x x x x
AM 18 Checking the PCBs and the brake resistor x x x x x x x x x x x x x x x x
AM 19 Checking the condenser fan x x x x x x x x
AM 20 Checking the compressor and the heat exchanger x x x x x x x x
AM 21 Checking the temperature sensors x x x x x x x x
AM 22 Evaluating the device data and deleting, if required x x x x x x x x x x x x x x x x
AM 23 Software update x x x x x x x x x x
AM 24 Performing a test run x x x x x x x x x x x x x x x x
Time required 60 min 70 min 90 min 120 min
 Maintenance
Centrifuge 5424 R 57
English (EN)

Spare parts kits are available for servicing some devices. The spare parts kits should be used
up completely every 24 months.

AM 01 Cleaning the device.

 Clean the centrifuge. Cleaning is described in the operating manual and in the
supplement sheets.
AM 02 Cleaning the condensation water tray and the hose.
1. Empty the condensation water tray and clean it.
2. Check that the tube is unobstructed.
3. Carefully blow the tube out with compressed air, if required.
AM 03 Checking the display.

 Check the display using the corresponding service function.

Pay attention to the following:
• Defective pixels
• Background lighting
 Replace display, if required
AM 04 Checking the keypad.

 Check the keypad using the corresponding service function. Press all of the keys.
 Check the tactility. The pressure on all of the keys must feel the same.
 Replace keypad, if required.
AM 05 Checking the housing

 Clean the housing. Cleaning is described in the operating manual and in the supplement
sheets.
 Check the housing for cracks. Liquid may enter the centrifuge through cracks.
 Check the housing for any damage that endangers safety.
 Replace the housing, if required.
AM 05 Checking stability

 Check the device feet for stability.

 If cracks have developed on the device feet, replace them.
AM 06 Checking the rotor bowl.
Cleaning of the rotor bowl is described in the operating manual.

 Inspect the rotor bowl for damage.

 Check the plastic rotor bowl for cracks.
 Clean the metal rotor bowl. The rotor bowl is made from stainless steel or has a special
coating.
 Check the rotor bowl for glass breakage and clean, if required.
 If samples with a high concentration of sodium chloride (NaCl) are used, spots may
appear which look like rust deposits. Remove any rust deposits with a polishing paste.
Rust deposits occur if the cleaning instructions recommended in the operating manual
were not followed.
 Replace the damaged rotor bowl.
 Maintenance
58 Centrifuge 5424 R
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AM 07 Cleaning and greasing the rotor bowl seal.

A description of the cleaning and care procedure can be found in the operating manual and
supplement sheets.
 Check the seal of the rotor bowl.
If the seal is defective, the lid will not close properly. More ambient air enters the rotor bowl.
The humidity entering the rotor bowl results in increased ice formation in the rotor bowl.
The cooling capacity of the centrifuge decreases.
Pay attention to the following:
• Seal with cracks
• Deformed seal
• Hardened seals
 Replace the damaged seal.
 Clean the seal of the rotor bowl.
 Maintain the seal of the rotor bowl, e. g. rub the seal with glycerin or grease.
AM 08 Checking the lid latch.

 Check the reed switch using the corresponding service function.

 Check the reed switch manually using a magnet.
AM 08 Checking the latch mechanism of the lid latch.

 Check the latch mechanism using the corresponding service function.

 Trigger the individual steps of the closing procedure by a separate keystroke.
Pay attention to the following parts:
• Lid latch
• Lid loops
• Slides and hooks
 Replace any worn parts.
AM 08 Checking the emergency release.

 Check the emergency release as described in the operating manual.

AM 09 Checking the lid springs and the lid shaft.

 Check the lid springs and the lid shaft for visible wear, e.g. chafe marks.
AM 09 Checking the gas spring.
The handling of the gas spring is described in the operating manual.

 Check the gas spring.

 Keep the centrifuge lid in the open position when the centrifuge is not in use.
 Always open the centrifuge lid completely. Do not allow shelving mounted above the
centrifuge to restrict its movement.
AM 10 Cleaning and greasing the lid latch.

 Clean the lid latch with a degreasing agent, e.g. alcohol. Do not use acidic substances.
 Maintenance
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English (EN)

AM 11 Checking the motor cap.

 Replace the seals of the motor cap every two years. Only insert the motor cap of the
Centrifuge 5430 R with silicone rubber E41.
 Check the seals.
Pay attention to the following:
• Cracks
• Damage
• Centered, tension-free installation
Cracks and damage are caused by:
• Incorrectly sealed sealing clamp on the aerosol-tight caps.
• Tubes and jammed parts in the rotor bowl during centrifugation.
• Ingress of liquids.
AM 11 Checking the speed sensor.

 Insert the rotor.

 Check the rotor detection and the rotor relation using the corresponding service function.
Note: Check the speed according to the OQ.
AM 12 Checking the motor sleeve.
The ingress of liquid causes extensive damage.
 Check the motor sleeve
Pay attention to the following:
• Cracks
• Damage
• Correct installation to ensure tightness
AM 13 Checking the motor.

 Remove the rotor from the centrifuge.

 Turn the motor shaft by hand. Operating noises and stiff movement indicate wear on the
bearing.
 Remove the housing.
 Check the bearing shield of the motor for corrosion and cracks.
 Replace any damaged parts.
AM 13 Checking the motor shaft.

Formation of grooves and scratches on the motor shaft:

• The rotor is removed from the centrifuge without due care.
• The rotor is placed in the centrifuge without due care.
• The rotor screw is not tightened.
 Remove the rotor.
 Check whether the motor shaft is worn.
 Check whether the motor shaft is clean.
 Replace the motor, if necessary.
 Maintenance
60 Centrifuge 5424 R
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AM 14 Checking the motor suspension.

If the shock mounts for motor are mounted horizontally, they are exposed to shearing forces
and the weight of the loaded rotor. The shock mounts may develop cracks or may become
deformed.
 Check the shock mounts for motor.
Pay attention to the following:
• Cracks
• Damage
• Deformation
 Replace the shock mounts for motor every two years.
 Only replace shock mounts for motor as a full set (3 pcs.).
AM 15 Checking the imbalance detection.
1. Adjust the imbalance switch as described in the Alignment/Adjustment chapter of the
service manual.
2. Using the corresponding customer service function, check whether the tolerance is
observed for the adjustment.
AM 16 Checking motor fan and device fan.

 Use a cleaning brush or compressed air to remove any dust and contamination. Remove
contamination with an industrial vacuum.
 Listen for running noises.
 Use the corresponding service function to select the different program steps for testing.
 If the centrifuge is used frequently, replace the fan as a precautionary measure.
AM 16 Checking the ventilation slots.

 Check all ventilation slots and openings. Clean them if required.

In addition, in Centrifuge 5804 and Centrifuge 5810:
 Insert the fixed-angle rotor.
 Switch on the device.
 Check whether the ventilation flap opens. Use the corresponding service function to do
this.
AM 17 Checking the fuse holder.

 Remove the fuses.

 Check the fuse holder contacts and the fuse mount for oxidation and deposits.
AM 18 Checking the PCBs and brake resistor.

 Use a cleaning brush or compressed air to remove any dust and contamination on the
PCB.
 Check the brake resistors using an Ohmmeter.
AM 19 Checking the condenser fan.

 Use a cleaning brush or compressed air to remove any dust and contamination.
 Listen for running noises.
 Check the different control stages using the corresponding service function.
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AM 20 Checking the compressor.

 Check switching the compressor on and off using the corresponding service function.
AM 20 Checking the heat exchanger.

 Use a cleaning brush or compressed air to remove any dust and contamination.
AM 21 Checking the temperature sensors.

 Display the current temperature on the display using the corresponding service function.
After the centrifuge has been at a standstill for an extended period with the lid open, the
displayed temperature must match the ambient temperature.
Note: Check the temperature according to the OQ.
AM 22 Evaluating the device data and deleting, if required.

 Call the following parameters with the corresponding service function or customer
service function:
• Error list
• Error history (last 4 errors)
• Number of activations
• Operating hours
• Total number of all rotor runs
• Number of runs per rotor type
Note: Which parameters are displayed depends on which centrifuge is checked.
AM 23 Software update
Software updates are provided free of charge as part of the maintenance to benefit from
improvements to the program.
A software update is performed by replacing an EPROM or via the USB interface.
You can download the latest software versions from the website
www.eppendorf-support.com.
AM 24 Performing a test run.
1. Insert the rotor with accessories.
2. Load the rotor with the maximum number of filled tubes.
3. Only refrigerated centrifuges: Start run by pressing the fast temp key. 6°C should be
reached quickly (depends on rotor, note the lowest temperature that can be achieved).
Additional information can be found in the operating manual.
 Maintenance
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10.3.3 Qualification Services

Inspections Group 1 Group 2 Group 3 Group 4

Non-refrigerated Refrigerated Non-refrigerated Refrigerated
No imbalance No imbalance Imbalance Imbalance
detection detection detection detection
• 5418 • 5415 R • 5430 • 5417 R
• 5424 • 5418 R • 5702 • 5427 R
• 5452 • 5424 R • 5804 • 5430 R
• 5453 • 5810 • 5702 R
• 5702 RH
• 5804 R
• 5810 R
• 5920 R
Service interval OQ Every 12 months
Time required on OQ 40 min 75 min 60 min 90 min
Time required on IQ 30 min 60 min 45 min 75 min

The IQ checklist and the OQ checklist are available on the Internet at www.eppendorf-support.com
(password required) or on request.

You can find descriptions for the measurements in the chapter “Device tests for centrifuges".
 Group 1 Group 2 Group 3 Group 4
Non-refrigerated Refrigerated Non-refrigerated Refrigerated
No imbalance Imbalance Imbalance Imbalance

10.3.4
detection detection detection detection

Additional Maintenance for Centrifuges

5418
5452
5452
5453
5430
5702
5804
5810

5415 R
5418 R
5424 R
5430 R
5920 R

5417 R 5427 R
5702 R 5702 RH
Nr. Service interval in month 24 24 24 24 24 24 24 24 24 24 24 24 24 24 5810 R 5804 R
24 24
PS 01 Replacing the lid springs and the gas springs x x x x x x x x x x x x x x
Time required 15 min 15 min 15 min 15 min
PS 02 Replacing the seal of the rotor bowl x x x x x x x x x x x x
Time required 15 min 20 min 20 min
PS 03 Replacing the motor suspension x x x x x x x x x x x x x x x x
Additional Maintenance

PS 04 Performing an Imbalance compensation x x x x x x x x x

Time required 15 min 20 min 45 min 60 min
PS 05 Software update x x x x x x x x x x
Time required 15 min 15 min 20 min
PS 06 Electrical safety check x x x x x x x x x x x x x x x x
Time required 15 min 15 min 15 min 15 min
PS 07 Speed check x x x x x x x x x x x x x x x x
Time required 10 min 10 min 10 min 10 min
PS 08 Timer check x x x x x x x x x x x x x x x x
Time required 10 min 10 min 10 min 10 min
PS 09 Temperature check x x x x x x x x x x x x x x x x
Time required 50 min 70 min 50 min 70 min
PS 10 Compulsory testing in Germany (BGR 500) x x x x
Verification every year in operating state about 90 min about 120 min
Verification every 3 years in dissembled state about 150 min about 200 min
English (EN)
Maintenance
Centrifuge 5424 R
63
 Maintenance
64 Centrifuge 5424 R
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Recommended service kits

• Spare parts kits are available for servicing some devices. The spare parts kits should be
used up completely every 24 months.

PS 01 Replacing the lid springs.

See chapter "Disassembly/assembly".
PS 01 Replacing the gas springs.
See chapter "Disassembly/assembly".
PS 02 Replacing the seal of the rotor bowl.
See chapter "Disassembly/assembly".
PS 03 Replacing the motor suspension.
See chapter "Disassembly/assembly".
PS 04 Performing an imbalance compensation.
See chapter "Alignment/adjustment" or Service function in chapter "Troubleshooting".
PS 05 Software update
A new software version makes extended functions available to the user, e.g. new rotors can
be used. A new version of the basic software is uploaded.

 Download current software versions from the website www.eppendorf-support.com.

 Install current software versions.
 To perform a software upgrade, EPROM may need to be replaced.
PS 06 Electrical safety check.
See chapter “Device tests for centrifuges”.
PS 07 Speed check.
See chapter “Device tests for centrifuges”.
PS 08 Timer check.
See chapter “Device tests for centrifuges”.
PS 09 Temperature check.
See chapter “Device tests for centrifuges”.
PS 10 Compulsory testing in Germany (BGR 500)
The accident prevention regulation BGR 500 (formerly VBG 7z) is valid in Germany.
Centrifuges with a rated power of > 500 W or a kinetic energy of > 10,000 Nm are subject to
testing.
The following Eppendorf AG centrifuges are subject to testing: Centrifuge 5804, 5804 R,
5810, 5810 R, 5810 RA, 5920 R.
These centrifuges must be tested once a year in their operating state and at least every 3
years in a dismantled state.
Rotors with a kinetic energy of ≤ 10,000 Nm are not subject to testing. Rotors that are used
in centrifuges subject to testing must be tested independent of their power output.
The owner of the centrifuge is obliged to document and keep the check results.
 Maintenance
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10.4 Device tests for centrifuges

10.4.1 Speed check

Measuring device
• Digital hand tachometer suitable for contactless measurement, or stroboscope
• Measurement range: 1 to ≥ 30,000 rpm
• Accuracy: ≤ 0.1%
1. Install the rotor.
2. Fully load the rotor with filled tubes.
3. Mark the rotor hub with a reflector (measuring device accessory).
4. Close the centrifuge lid.
5. Start the centrifuge with the desired speed. Let the centrifuge run until the speed has stabilized.
6. Measure the speed via the monitoring glass in the centrifuge lid using the operating manual of the
measuring device.
7. Note the measuring result.
8. If required, repeat steps 5 to 7 for other rotational speeds.

A recommendation on the rotational speeds to be tested, and their tolerances, can be found in the technical
data or OQ of the corresponding centrifuge.

10.4.2 Temperature check

Ambient temperature: 23°C ± 2°C

Measuring device
• Thermometer with a temperature sensor and a low internal heat capacity, e.g. thermocouple, max. Ø
0.5 mm)
• Measurement range: 0 °C to ≥ 100 °C
• Accuracy: ≤ 0.1°C
Auxiliary equipment
• Rotor
• Tubes that fit the rotor, with min. volume of 1.5 mL

10.4.2.1 Refrigerated centrifuges

1. Install the rotor and load it symmetrically with tubes.

2. Fill the tubes with ethylene glycol.
3. Set the maximum rotational speed.
4. Set the minimum temperature that can be achieved (depends on the rotor).
5. Precool the centrifuge and rotor with tubes and with adapters, if required, using fast temp (refer to the
operating manual for the centrifuge).
6. Start the centrifuge with the previously set max. speed and min. temperature.
7. Wait for 60 min. Then stop the centrifuge.
8. Measure the temperature in the center of the tubes immediately after opening.
9. Compare the sample temperature with the temperature displayed by the centrifuge.
 Maintenance
66 Centrifuge 5424 R
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10.4.2.2 Non-refrigerated centrifuges

For non-refrigerated centrifuges, sample heating is checked against the ambient temperature.

1. Install the rotor and load it symmetrically with tubes.

2. Fill the tubes with ethylene glycol.
3. Set the maximum rotational speed.
4. Start the centrifuge and let it run for at least 45 min.
5. Measure the ambient temperature.
6. Stop the centrifuge and measure the temperature in the center of the tubes immediately after opening.
7. Compare the ambient temperature and sample temperature.

A recommendation on the temperatures to be tested, and their tolerances, can be found in the technical
data or OQ of the corresponding centrifuge.

10.4.3 Timer check

Measuring device
• Stopwatch
• Accuracy: ≤ 0.1 seconds
1. Set the run time to continuous operation.
2. Start the centrifuge and stopwatch at the same time.
3. Allow the centrifuge to run at least 5 min.
4. Compare the time displayed by the centrifuge with the time measured by the stopwatch.

A recommendation on the times to be tested, and their tolerances, can be found in the technical data or OQ
of the corresponding centrifuge.
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10.4.4 Electrical safety check

Effective standard
• Pre-IEC 62638 (international)
• VDE 701/702 (Germany)
• NFPA 99 (USA)

Notes concerning the measuring procedure:

• The electrical safety check may only be carried out by a suitably trained electrician.
• After repairs or after work on the electrical system of the centrifuge (incl. protective earth conductor
system) the electrical safety must be tested.
• The electrical safety must be checked at regular intervals (e.g. every 12 months).
• Measure on a grounded, metallic point that is not insulated and can be touched from the outside (e.g.
housing screws).
• The leakage current or resistance of the PE conductor cannot be measured at the centrifuge lid, rotor, or
motor shaft.
• Never measure at any interfaces that may be present. The centrifuge electronics could become
damaged.
• Due to the internal electrical design of the centrifuge, the insulation resistance cannot be measured
correctly.
• Due to the internal electrical design of the centrifuge, the equivalent leakage current cannot be
measured correctly.
→ Always measure the real leakage current (direct measurement or differential current measurement).

10.5 Interface test

The function of the USB interface can be tested using the "CefuComTest" program and a laptop/PC.

To test the interface, the "CefuComTest" software and corresponding driver must be installed on your
laptop/PC. The "CefuComTest", driver, and instructions can be downloaded from the Support webpage:
www.eppendorf-support.com (password required).

Read the installation and operating instructions for the "CefuComTest" carefully before
starting downloading, installation or the interface test!
For the interface test, the corresponding service function must be activated. Observe the
description of the service function in chapter 5.
 Maintenance
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 Technical data
Centrifuge 5424 R 69
English (EN)

11 Technical data
11.1 Power supply

Centrifuge 5424 R

Mains power connection 230 V, 50 to 60 Hz

120 V, 50 to 60 Hz
100 V, 50 to 60 Hz
Current consumption: 1.6 A (230 V)
3.2 A (120 V)
3.4 A (100 V)
Power consumption: max. 350 W
EMC: Interference emission (radio interference) EN 61326 - category B
EMC: Noise immunity EN 61326
Overvoltage category: II
Fuses: 3.15 AT (230 V)
6.3 AT (120 V / 100 V)

11.2 Ambient conditions

Environment: For indoor use only.

Ambient temperature: Centrifuge 5424 R: 10 to 40°C
Max. relative humidity: 10 to 75 %, non-condensing humidity
Atmospheric pressure: Use up to an altitude of 2000 m above MSL.
Degree of contamination: 2

11.3 Weight/dimensions

Centrifuge 5424 R

Dimensions: Width: 290 mm (11.42 in.)

Depth: 480 mm (18.90 in.)
Height: 260 mm (10.24 in.)
Weight without rotor: 21.0 kg (46.3 lb)
Noise level: < 54 dB(A) *

*) The noise level was measured according to DIN EN ISO 3745 frontally in a sound measuring room with
accuracy class 1 at a distance of 1 m from the device and at lab bench height.
 Technical data
70 Centrifuge 5424 R
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11.4 Application parameters

11.4.1 Centrifuge 5424 R

Tab. 11-1: Acceleration time and braking time according to DIN 58 970
Rotor Without soft ramp With soft ramp SOFT
Acceleration time Deceleration time Acceleration time Deceleration time
FA-45-24-11 15 s 16 s 26 s 8s

These values were calculated for 230 V at 23°C.

Run time 30 s - 9:59 h, as well as infinity (oo),

up to 10 min run time adjustable in 0.5 min
increments, then in 1 min increments.
Temperature -10°C - 40°C
Relative centrifugal force (RCF) 1 – 21.130 x g,
adjustable in 50 x g increments.
Speed: 100 – 15,000 rpm,
adjustable in 100 rpm increments.
Maximum load 24 micro test tubes of 2.0 mL each.
Maximum kinetic energy 4070 Nm
Compulsory test log book No
Allowable density of the centrifuge material (at 1.2 g/mL
maximum g-force/speed and maximum load)

11.5 Tolerances

All data applies to an ambient temperature of 23 °C ± 2 °C.

Parameters Tolerance
Speed (1000 rpm up to max. speed) ± 100 rpm
Temperature At 6 °C ± 2 °C
Timer At 5 min run time ± 2 s
 Ordering information
Centrifuge 5424 R 71
English (EN)

12 Ordering information
12.1 Images of spare parts
Abb. 12-1: Centrifuge 5424 R, housing and lid

2-3

3-3

2-2
2-4

1-2

1-3

1-1

1-8

Fig. 12-1: Centrifuge 5424 R, housing and lid

 Ordering information
72 Centrifuge 5424 R
English (EN)

Abb. 12-2: Centrifuge 5424 R, lid axle and lid springs

2-7

2-6

2-1

2-5

2-4

2-3

Fig. 12-2: Centrifuge 5424 R, lid axle and lid springs

 Ordering information
Centrifuge 5424 R 73
English (EN)

Abb. 12-3: Centrifuge 5424 R, rotor bowl

1-6

4-4

4-5 5-4

Fig. 12-3: Centrifuge 5424 R, rotor bowl

 Ordering information
74 Centrifuge 5424 R
English (EN)

Abb. 12-4: Centrifuge 5424 R, bottom view

1-5

1-4 1-4

1-7

Fig. 12-4: Centrifuge 5424 R, bottom view

 Ordering information
Centrifuge 5424 R 75
English (EN)

Abb. 12-5: Centrifuge 5424 R, right side view with upper part of the housing removed

3-5

3-1

3-4 7-2 4-7 4-6

Fig. 12-5: Centrifuge 5424 R, right side view with upper part of the housing removed
 Ordering information
76 Centrifuge 5424 R
English (EN)

Abb. 12-6: Centrifuge 5424 R, rear view with upper part of the housing removed

7-3

5-3

6-1 7-1

Fig. 12-6: Centrifuge 5424 R, rear view with upper part of the housing removed
 Ordering information
Centrifuge 5424 R 77
English (EN)

Abb. 12-7: Centrifuge 5424 R, electronics fan and reed switch

3-2

6-2

Fig. 12-7: Centrifuge 5424 R, electronics fan and reed switch

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78 Centrifuge 5424 R
English (EN)

Abb. 12-8: Centrifuge 5424 R, motor

5-1

5-2

Fig. 12-8: Centrifuge 5424 R, motor

 Ordering information
Centrifuge 5424 R 79
English (EN)

Abb. 12-9: Centrifuge 5424 R, PCB Display/Operation

Fig. 12-9: Centrifuge 5424 R, PCB Display/Operation

Abb. 12-10: Centrifuge 5424 R, PCB Control/frequency converter

Fig. 12-10: Centrifuge 5424 R, PCB Control/frequency converter

 Ordering information
80 Centrifuge 5424 R
English (EN)

Abb. 12-11: Centrifuge 5424 R, PCB Lid Switch

Fig. 12-11: Centrifuge 5424 R, PCB Lid Switch

Abb. 12-12: Centrifuge 5424 R, PCB Interface

Fig. 12-12: Centrifuge 5424 R, PCB Interface

 Ordering information
Centrifuge 5424 R 81
English (EN)

12.2 Spare parts

12.2.1 Housing

Fig. Item Order no. Description

(International)
Upper part of housing
12-1 1-1 5404 850.026 Rotary knob
12-1 1-1 5404 850.034 Keypad
Keypad
12-1 1-2 5404 850.042 Rotary knob
12-1 1-2 5404 850.050 Keypad
Rotary knob
12-1 1-3 5702 852.040 1 piece
Device foot
12-4 1-4 5404 850.409 4 pieces
Vent cap ground plate
12-4 1-5 5404 850.069 3 pieces
12-3 1-6 5404 850.077 Sealing for bowl
12-4 1-7 5404 850.085 Tray for condensation water
12-1 1-8 5404 850.018 Cap for emergency release

12.2.2 Lid

Fig. Item Order no. Description

(International)
Lid spring
12-2 2-1 5404 850.093 cpl. set
12-1 2-2 5404 850.107 Lid covering
Lid panel
12-1 / 12-2 2-3 5404 850.115 with monitoring glass
12-1 / 12-2 2-4 5404 850.379 Monitoring glass
12-2 2-5 5404 850.123 Lid axle and lid guide bar
Guide sleeve
for lid axle
12-2 2-6 5404 850.131 2 pieces
Lid buffer
12-2 2-7 5409 850.385 cpl. Set
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82 Centrifuge 5424 R
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12.2.3 Lid latch

Fig. Item Order no. Description

(International)
12-5 3-1 5404 850.140 Motor for lid latch
12-7 3-2 5404 850.158 Reed switch
12-1 3-3 5404 850.360 Reed magnet
12-5 / 12-11 3-4 5404 850.166 PCB Lid switches
Tension spring
12-5 3-5 5404 850.417 set of 2 pieces

12.2.4 Electronics

In some cases, the software/firmware version of the PCB(s) does not match the current status.

 After replacing the printed circuit board(s) check the software version and update it, if
required.

Fig. Item Order no. Description

(International)
PCB Display/Operating
12-9 4-1 5404 850.174 Rotary knob
12-9 4-1 5404 850.182 Keypad
PCB Control/Converter
12-10 4-2 5404 850.190 230 V
12-10 4-2 5404 850.204 100/120 V
12-12 4-3 5404 850.212 PCB Interface
Rubber sleeve
12-3 4-4 5404 850.220 for temperature sensor
Temperature sensor
12-3 4-5 5811 643.000 rotor bowl
12-5 4-6 5428 850.434 condenser
Mains input module
12-5 4-7 5404 850.239 100/120/230 V
Fuse
--- 4-8 5424 852.122 3,15 A T (230 V), set of 2
--- 4-8 5424 852.130 6,3 A T (120 V/100 V), set of 2
 Ordering information
Centrifuge 5424 R 83
English (EN)

12.2.5 Drive

Fig. Item Order no. Description

(International)
Motor
12-8 5-1 5404 850.247 100/120/230 V
Motor suspension
12-8 5-2 5425 300.107 Set of 3
Brake resistor
12-6 5-3 5404 850.263 100/120/230 V
12-3 5-4 5404 850.271 Motor sealing

12.2.6 Fan

Fig. Item Order no. Description

(International)
Fan
12-6 6-1 5404 850.280 condenser
12-7 6-2 5404 850.298 electronic

12.2.7 Cooling

Fig. Item Order no. Description

(International)
Compressor
12-6 7-1 5404 850.301 100-240 V, 50-60 Hz
12-5 7-2 5404 850.310 Dryer cartridge
Solenoid valvel
12-6 7-3 5404 850.344 100 V
12-6 7-3 5404 850.336 120 V
12-6 7-3 5404 850.328 230 V

12.2.8 Accessories

Fig. Item Order no. Description

(International)
--- 8-1 5404 850.352 Packing
Rotor key
--- 8-2 5416 301.001 Standard
USB software cable
--- 8-3 5428 850.280 USB-A/USB-B
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12.2.9 Rotors

Fig. Item Order no. Description

(International)
Rotor FA-45-24-11
--- 9-1 5424 702.007 aerosol-tight, aluminum, 45° angle, 24 places, max. tube diameter
11 mm, incl. rotor lid (aluminum)
Replacement lid for rotor FA-45-24-11
--- 9-2 5424 703.003 aerosol-tight, aluminum
Rotor FA-45-24-11-Special
--- 9-3 5424 700.004 aerosol-tight, aluminum, PTFE-coated, 45° angle, 24 places, max.
tube diameter 11 mm, incl. rotor lid (aluminum)
Replacement lid for rotor FA-45-24-11-Special
--- 9-4 5424 701.000 aerosol-tight, aluminum, PTFE-coated
Rotor F-45-18-11-Kit
--- 9-5 5424 706.002 aluminum, 45° angle, 18 places, max. tube diameter 11 mm,
incl. rotor lid (polypropylene)
Spare lid for rotor F-45-18-11-Kit
--- 9-6 5424 707.009 Polypropylene
Rotor F-45-32-5-PCR
--- 9-7 5424 704.000 incl. rotor lid (aluminum)
Spare lid for rotor FA-45-32-5-PCR
--- 9-8 5424 708.005 aluminum

12.3 Suggested spare parts

For the initial equipment of your service warehouse, we recommend the following spare parts:

Fig. Item Order no. Description

(International)
12-5 3-1 5404 850.140 Motor for lid latch
PCB Control/Converter
12-10 4-2 5404 850.190 230 V
12-10 4-2 5404 850.204 100/120 V
Fuse
--- 4-8 5424 852.122 3,15 A T (230 V), set of 2
--- 4-8 5424 852.130 6,3 A T (120 V/100 V), set of 2
Motor
12-8 5-1 5404 850.247 100/120/230 V
Motor suspension
12-8 5-2 5425 300.107 Set of 3
Brake resistor
12-6 5-3 5404 850.263 100/120/230 V
 Technical information
Centrifuge 5424 R 85
English (EN)

13 Technical information
 Technical information
86 Centrifuge 5424 R
English (EN)
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