UTTAR PRADESH RAJARSHI TANDON OPEN UNIVERSITY
SHANTIPURAM, SECTOR-F, PHAPHAMAU, PRAYAGRAJ-2110021
ASSIGNMENT PAPER
Session: 2024-25 Max. Marks: 30
Program Name: M.Sc.- Biochemistry
Course Code: PGBCH-111 N Course Name: Enzymology and Enzyme Technology
SECTION -A 2*6=12 marks
Q. No. Short answer type question (approx. 200 -300 words) Marks
1 What do you understand by electrophoretic technique for enzyme 2
purification? Write the principle behind this method.
2 What are allosteric enzymes? Briefly explain its mechanism of action. 2
3 Discuss the Criteria for enzyme homogeneity? 2
4 What are the methods involved in enzyme immobilization? Briefly 2
explain.
5 Briefly explain- 2
a) Suicide inhibitors
b) Artificial enzymes
6 Write short note on- 2
a) Catalytic antibodies
b) Cofactors
c) Ribozymes
d) Enzyme specificity
SECTION -B Sh6*3=18
marks
Long answer type question (approx. 500 -800 words) Marks
7 How is centrifugation utilized in the study and production of enzymes, 6
and what specific roles does it play in the isolation, purification, and
analysis of enzyme samples?
8 Explain the Michaelis-Menten equation, its significance in 6
enzymology, and how it describes the relationship between substrate
concentration and the rate of enzymatic reactions?
UTTAR PRADESH RAJARSHI TANDON OPEN UNIVERSITY
SHANTIPURAM, SECTOR-F, PHAPHAMAU, PRAYAGRAJ-2110021
ASSIGNMENT PAPER
Session: 2024-25 Max. Marks: 30
Program Name: M.Sc.- Biochemistry
Course Code: PGBCH-112 N Course Name: Immunology
SECTION -A 2*6=12 marks
Q. No. Short answer type question (approx. 200 -300 words) Marks
1 Explain the acquired immunodeficiency. 2
2 Explain the Antigens processing and presentation 2
3 Describe the types of common vaccines for human. 2
4 Write the notes maturation and selection of T cells 2
5 What do you understand by clonal selection theory? 2
6 Explain the structure and functions of antibody. 2
SECTION -B Sh6*3=18
marks
Long answer type question (approx. 500 -800 words) Marks
7 Write the short notes on the following. 6
a) Natural killer cells (NK cells)
b) Vaccines
c) Adaptive and cellular immunity
8 Describe the types of immunity- innate, acquired, adaptive and 6
cellular immunity.
9 Describe the architecture of immune system. 6
UTTAR PRADESH RAJARSHI TANDON OPEN UNIVERSITY
SHANTIPURAM, SECTOR-F, PHAPHAMAU, PRAYAGRAJ-2110021
ASSIGNMENT PAPER
Session: 2024-25 Max. Marks: 30
Program Name: M.Sc.- Biochemistry
Course Code: PGBCH-113 N Course Name: Basic Biotechnology
SECTION -A 2*6=12 marks
Q. No. Short answer type question (approx. 200 -300 words) Marks
1 Write short note on the below mentioned techniques – 2
a- Biotransformation
b- Metabolic engineering for metabolite over production
2 Briefly explain- a-Gene therapy b-Hybridoma technology 2
3 Write a note on gene transfer method in plants. 2
4 Briefly explain the concept of recombinant DNA technology (RDT). 2
5 Explain the role of biotechnology to improve yield and nutritional 2
values of crops.
6 What do you understand by plant tissue culture? What are the steps 2
involved in plant tissue culture. What are its applications?
SECTION -B Sh6*3=18
marks
Long answer type question (approx. 500 -800 words) Marks
7 Discuss about the scope and importance of biotechnology. 6
8 Briefly describe GM (Genetically modified) foods and the risk 6
associated with these GM foods.
9 Give an account of immunological techniques involved in medical 6
biotechnology.
UTTAR PRADESH RAJARSHI TANDON OPEN UNIVERSITY
SHANTIPURAM, SECTOR-F, PHAPHAMAU, PRAYAGRAJ-2110021
ASSIGNMENT PAPER
Session: 2024-25 Max. Marks: 30
Program Name: M.Sc.- Biochemistry
Course Code: PGBCH-116 N Course Name: Bio-safety and IPR
SECTION -A 2*6=12 marks
Q. No. Short answer type question (approx. 200 -300 words) Marks
1 Write short notes on the following. a) Antibiotic resistance. b) Gene 2
flow
2 Explain benefits of transgenic to human health, society and the 2
environment.
3 Explain convention on biological diversity. 2
4 Explain radiation safety. 2
5 Explain Cartagena protocol on biosafety. 2
6 Explain gene flow in natural and artificial ecologies. 2
SECTION -B Sh6*3=18
marks
Long answer type question (approx. 500 -800 words) Marks
7 Describe genetically modified foods (GMF). 6
8 What do you mean by patent, intellectual property right (IPR) and 6
international agreement?
9 Write short notes on the following 6
a) Eco-labeling
b) Ecomark
c) GMO
UTTAR PRADESH RAJARSHI TANDON OPEN UNIVERSITY
SHANTIPURAM, SECTOR-F, PHAPHAMAU, PRAYAGRAJ-2110021
ASSIGNMENT PAPER
Session: 2024-25 Max. Marks: 30
Program Name: M.Sc.- Biochemistry
Course Code: PGBCH-117 N Course Name: Environmental Toxicology and Occupational
Health Hazardous
SECTION -A 2*6=12 marks
Q. No. Short answer type question (approx. 200 -300 words) Marks
1 Write shote notes on- 2
a) Acute exposure
b) Chronic exposure
2 Explain renal toxicity? 2
3 Discuss the accidental poisoning? 2
4 Discuss the pesticide and automobile emission. 2
5 Discuss the internal poisoning? 2
6 Explain mineral toxicity? 2
SECTION -B Sh6*3=18
marks
Long answer type question (approx. 500 -800 words) Marks
7 Discuss the history and scope of toxicology. 6
8 Discuss carcinogenesis and types of carcinogens. 6
9 Discuss the laws and regulations governing toxicants. 6
UTTAR PRADESH RAJARSHI TANDON OPEN UNIVERSITY
SHANTIPURAM, SECTOR-F, PHAPHAMAU, PRAYAGRAJ-2110021
ASSIGNMENT PAPER
Session: 2024-25 Max. Marks: 30
Program Name: M.Sc.- Biochemistry
Course Code: PGBCH-118 N Course Name: Genetic Engineering
SECTION -A 2*6=12 marks
Q. No. Short answer type question (approx. 200 -300 words) Marks
1 Write short notes on the following 2
a) c-DNA library
b) Gilbert sequencing
2 What do you know by gene expression in prokaryotes? 2
3 What do you understand by gene expression in Eukaryotes? 2
4 Explain DNA fingerprinting.? 2
5 Explain uses of monoclonal antibodies. 2
6 Discuss the applications of transgenic plants. 2
SECTION -B Sh6*3=18
marks
Long answer type question (approx. 500 -800 words) Marks
7 Discuss the synthesis of c-DNA from m RNA. 6
8 Write short notes on- 6
a) M13 Phage
b) pUC8
c) pBR322
9 Discuss real time PCR in detail. 6
UTTAR PRADESH RAJARSHI TANDON OPEN UNIVERSITY
SHANTIPURAM, SECTOR-F, PHAPHAMAU, PRAYAGRAJ-2110021
ASSIGNMENT PAPER
Session: 2024-25 Max. Marks: 30
Program Name: M.Sc.- Biochemistry
Course Code: PGBCH-120 N Course Name: Microbiology and Toxicology
SECTION -A 2*6=12 marks
Q. No. Short answer type question (approx. 200 -300 words) Marks
1 Explain the significance of major histo-compatibility complex (MHC) 2
2 How do you differentiate between synchronous growth and 2
continuous culture?
3 Write short notes on a) Conjugation, b) Transduction, c) 2
Recombination
4 What do you know by Denaturing Gradient Gel Electrophoresis 2
(DGGE)?
5 Discuss the Environmental Xenobiotic, 2
6 Write the about microbial growth. 2
Sh6*3=18
marks
Long answer type question (approx. 500 -800 words) Marks
7 What do you mean sterilization? Write the theory and practice of 6
sterilization.
8 Write the general characteristics of primary domains and of 6
taxonomic groups belonging to Bacteria.
9 What are antimicrobial agents? Discuss about the Penicillins and 6
Cephalosporins
UTTAR PRADESH RAJARSHI TANDON OPEN UNIVERSITY
SHANTIPURAM, SECTOR-F, PHAPHAMAU, PRAYAGRAJ-2110021
ASSIGNMENT PAPER
Session: 2024-25 Max. Marks: 30
Program Name: M.Sc.- Biochemistry
Course Code: PGBCH-121 N Course Name: Industrial Biotechnology
SECTION -A 2*6=12 marks
Q. No. Short answer type question (approx. 200 -300 words) Marks
1 Write short notes on the following. a) Sterilization. b) Pasteurization 2
2 Describe whole cell immobilization. 2
3 Write a short note on bioreactor. 2
4 Write short notes on the following. 2
a) filtration
b) liquid-liquid extraction
5 Write a short note on enzymes used in baking and pharmaceutical 2
industry.
6 What are the roles of micro elements in industrial process? 2
SECTION -B Sh6*3=18
marks
Long answer type question (approx. 500 -800 words) Marks
7 Describe different enzymes with their general properties. 6
8 What do you mean by fermentation? Describe different types of 6
fermentation process.
9 Write short notes on the following. a) Fermentation. b) Centrifugation 6
c) Chromatography
UTTAR PRADESH RAJARSHI TANDON OPEN UNIVERSITY
SHANTIPURAM, SECTOR-F, PHAPHAMAU, PRAYAGRAJ-2110021
ASSIGNMENT PAPER
Session: 2024-25 Max. Marks: 30
Program Name: M.Sc.- Biochemistry
Course Code: PGBCH-122N Course Name: Bioinformatics
SECTION -A 2*6=12 marks
Q. No. Short answer type question (approx. 200 -300 words) Marks
1 Explain the protein data bank. 2
2 What do you understand by MSA (multiple sequence alignment)? 2
3 Discuss the recourses of EMBL. 2
4 Describe the methods involved in phylogeny. 2
5 Explain the classification scheme of biological databases. 2
6 Explain the protein 3D structure and its data base. 2
SECTION -B Sh6*3=18
marks
Long answer type question (approx. 500 -800 words) Marks
7 Write short notes on the following. 6
(a) SCOP
(b) BLAST
(c) NCBI
8 Describe the aim of bioinformatics and its scopes. 6
9 Discuss the importance of bioinformatics studies in modern biology. 6
