UV SPECTROPHOTOMETER
Q1 What is principle of UV spectrophotometer?
Ans When a beam of monochromatic light passes through a
homogeneous absorbing medium, the rate of decrease in
intensity of light is directly proportional to the concentration
and path length of cell.
Q2 What is Beer's Law?
Ans Absorption is directly proportional to concentration of
medium Means, More the conc. more will be absorption
Q3 What is Lambert's Law?
Ans Absorption is directly proportional to the pathlength of
cell. Means, More the pathlength size more will be absorption
Q4 What is UV scanning range?
Ans: 200nm-400nm
Q5 What is Visible scanning range?
Ans Visible range is 400nm - 800nm
Q6 How many types of cuvettes used in UV visible
spectrophotometer?
Ans: Mostly 4 types of cuvettes used
1. optical glass or Pyrex Glass
2. UV Quartz
3. IR Quartz
4. Sapphire
Q7 Which cuvette used in UV scanning range?
Ans: Quartz cuvette used in UV scanning range
Q8 Which cuvette used for visible scanning range?
Ans: Glass cuvette used in Visible scanning range
Q9 Can we perform Quantitative & Qualitative analysis
on UV visible Spectrophotometer?
Ans: Yes, we can perform both the analysis
Q10 What is measuring unit of UV visible
spectrophotometer?
Ans: Absorbance
Q11 What is light source for UV region?
Ans: Deuterium Lamp used as a light source in UV region
Q12 What is light source for Visible region?
Ans: Halogen Lamp used as light source in Visible region
Q13 Which Lamp used for UV-Visible spectrophotometer?
Ans: Xenon Lamp used for UV-Visible spectrophotometer
Q14 How many components in UV-Visible
spectrophotometer?
Ans: Total 5 components in UV-Visible spectrophotometer.
Q15 What are the components in UV-Visible
spectrophotometer?
Ans: 1. Light source
2. Monochromator
3. Sample holder
4. Detector
5. Recorder
Q16 Why Quartz cuvettes used in UV scanning range?
Ans: Because Quartz cuvette will not absorb light.
Q17 What is General Chapter number of UV spectroscopy
as per USP?
Ans USP General Chapter < 857>
